Auxiliary device for engineering macrophage culture and use method thereof

Abstract

The invention discloses an auxiliary device for engineering macrophage culture and a use method of the auxiliary device, and particularly relates to the technical field of cell culture.The auxiliary device is provided with a driving assembly, a first gear, a second gear, a rotating seat, a control valve, a rotating gear, a liquid storage tank, a collecting cylinder and a toothed bar; when the liquid storage box rotates close to the cultivation box, the magnetic plate moves away from the magnetic block, at the moment, the second elastic assembly controls the toothed bar to move, the toothed bar controls the rotating gear and the control valve to rotate, the control valve is opened, and the nutrient solution in the liquid storage box is automatically added into the cultivation box. According to the device, the nutrient solution is discharged and added automatically, manual operation steps are not needed, no infectious microbes enter the process, the replacement operation efficiency is guaranteed, and the safety of culture operation is guaranteed to a certain extent.

Description

Technical Field

[0001] The present invention relates to the technical field of cell culture, and more specifically, the present invention relates to an auxiliary device for engineering macrophage culture and a method for using the same. Background Art

[0002] During the culture process of macrophages, the nutrient solution in the incubator needs to be replaced regularly. The replacement operation of the nutrient solution is all carried out manually. The efficiency of the replacement operation is affected by proficiency. At the same time, the manual operation process is more likely to cause other germs to enter and is prone to contamination. Therefore, it brings inconvenience to the replacement of the nutrient solution. An auxiliary device for engineering macrophage culture and a method for using the same are needed to solve the above problems. Summary of the Invention

[0003] In order to overcome the above defects, the present invention provides an auxiliary device for engineering macrophage culture and a method for using the same. The technical problem to be solved by the present invention is the inconvenience in the replacement operation of the nutrient solution, which affects the efficiency and safety.

[0004] To achieve the above object, the present invention provides the following technical solution: An auxiliary device for engineering macrophage culture, including a liquid addition auxiliary box and a culture box. The culture box is disposed through one side of the liquid addition auxiliary box. A sealed contact seat is connected inside the liquid addition auxiliary box. A rotating seat is connected outside the sealed contact seat. A rotating shaft is connected to the rotating seat. A driving device is disposed outside the rotating shaft. The driving device is connected inside the liquid addition auxiliary box. A first placement groove is formed outside the rotating seat. A second placement groove is formed outside the rotating seat. A liquid collection device is connected inside the first placement groove. A liquid storage tank is connected inside the second placement groove. A positioning groove is formed on the liquid storage tank. An elastic positioning device is connected inside the positioning groove. The elastic positioning device is disposed through the rotating seat. A liquid injection device is connected below the liquid storage tank. The liquid injection device is located inside the second placement groove. A magnetic plate is disposed inside the sealed contact seat corresponding to the position of the liquid injection device. An extrusion ring is connected to the elastic positioning device. The extrusion ring is connected inside the liquid addition auxiliary box.

[0005] As a further aspect of the present invention: A sealed door is disposed outside the liquid addition auxiliary box. An ultraviolet disinfection lamp is connected to one side of the sealed door inside the liquid addition auxiliary box. The position of the ultraviolet disinfection lamp corresponds to the positions of the liquid storage tank and the liquid collection device.

[0006] As a further aspect of the present invention: A limiting column is connected outside the liquid storage tank. A limiting groove is formed inside the second placement groove. The limiting column is slidably disposed inside the limiting groove. A bearing is sleeved outside the rotating shaft. The bearing is connected inside the liquid addition auxiliary box. An outlet pipe is communicated outside the liquid addition auxiliary box.

[0007] As a further solution of the present invention: a connection groove is provided in the second placement groove, the liquid injection device is arranged in the connection groove, and the sealing contact seat is arc-shaped.

[0008] As a further solution of the present invention: the liquid collection device includes a collection cylinder, the collection cylinder is connected in the first placement groove, a filter plate is connected through the outside of the collection cylinder, the position of the filter plate corresponds to the position of the culture box, and a drain pipe is connected under the collection cylinder.

[0009] As a further solution of the present invention: the elastic positioning device includes a sliding sleeve, the sliding sleeve is connected through the rotating seat, a positioning rod is slidably connected in the sliding sleeve, the positioning rod is arranged in the positioning groove, an extension piece is connected outside the positioning rod, a first elastic component is sleeved outside the positioning rod, one end of the first elastic component is connected with the sliding sleeve, the other end of the first elastic component is connected with the extension piece, the top end of the positioning rod is connected with a contact block, and the contact block is arranged under the extrusion ring.

[0010] As a further solution of the present invention: the liquid injection device includes a control valve, the control valve is arranged under the liquid storage tank, a liquid injection pipe is connected under the control valve, the liquid injection pipe is communicated with the liquid storage tank through the control valve, a rotating gear is arranged outside the control valve, and a rack is meshed outside the rotating gear.

[0011] As a further solution of the present invention: one end of the rack is connected with a sliding block, the sliding block is slidably connected in the connection groove, a second elastic component is connected outside the sliding block, the other end of the second elastic component is connected with the connection groove, the other end of the rack is connected with a magnetic block, the surfaces of the magnetic block and the magnetic plate close to each other have the same magnetism, and the position of the magnetic block corresponds to the position of the magnetic plate.

[0012] As a further solution of the present invention: the driving device includes a driving component, the driving component is connected in the liquid adding auxiliary box, an output shaft of the driving component is connected with a first gear, and a second gear is meshed outside the first gear, and the second gear is connected outside the rotating shaft.

[0013] A method for using an auxiliary device for engineering macrophage culture includes the following steps:

[0014] When it is necessary to replace the nutrient solution in the culture box, directly control the driving component to work. The driving component controls the second gear and the rotating seat to rotate through the first gear. At this time, the collection cylinder rotates to a position close to the culture box, and at the same time, the filter plate is located in the culture box. At this time, the first gear is disengaged from the second gear, and the nutrient solution in the culture box enters the collection cylinder through the filter plate. At this time, the collection of waste liquid is completed. When the contact block contacts the extrusion ring, it is extruded, and at this time, control the positioning rod to slide downward;

[0015] The positioning rod enters the positioning groove to complete the limit between the liquid storage tank and the rotating seat. The first gear continues to rotate and meshes with the second gear to control the rotation of the second gear and the rotating seat. At this time, the collection cylinder is controlled to rotate away from the culture box. The liquid storage tank rotates to the position corresponding to the culture box, and the magnetic block moves away from the magnetic plate, and the magnetic force between the two disappears. At the same time, the second elastic component controls the movement of the sliding block and the rack. The rack uses meshing to control the rotation of the rotating gear and the control valve, and the control valve opens. At this time, the nutrient solution in the liquid storage tank is injected into the culture box through the control valve and the liquid injection pipe to complete the liquid change process. When the first gear meshes with the second gear again, the rotating seat and the liquid storage tank are controlled to rotate at this time. The magnetic block moves close to the magnetic plate, and the magnetic force between the two controls the rack to extend into the connection groove. The rack drives the rotating gear and the control valve to rotate, and the control valve closes.

[0016] The beneficial effects of the present invention are as follows:

[0017] 1. By setting the driving component, the first gear, the second gear, the rotating seat, the control valve, the rotating gear, the liquid storage tank, the collection cylinder, and the rack, when the driving component works, the rotation of the second gear and the rotating seat can be controlled through the first gear. When the collection cylinder rotates to the position corresponding to the culture box, the nutrient solution in the culture box enters the collection cylinder through the filter plate to complete the collection of the waste liquid. When the liquid storage tank rotates close to the culture box, the magnetic plate moves away from the magnetic block. At this time, the second elastic component controls the movement of the rack, and the rack controls the rotation of the rotating gear and the control valve. The control valve opens, and the nutrient solution in the liquid storage tank is automatically added to the culture box, so that the discharge and addition of the nutrient solution are automatically completed without manual operation steps. No miscellaneous bacteria enter during the process, and the replacement operation efficiency is guaranteed, and the safety of the culture operation is ensured to a certain extent;

[0018] 2. By setting the extrusion ring, the contact block, the positioning rod, the positioning groove, the first elastic component, the limiting rod, and the limiting groove, when the rotating seat rotates to drive the liquid storage tank to rotate to the position corresponding to the sealing door, the extrusion ring and the contact block are separated at this time. The first elastic component uses its own elastic force to control the positioning rod to move upward, and the positioning rod is separated from the positioning groove. At this time, the liquid storage tank can be directly taken out through the sealing door, and the ultraviolet disinfection lamp disinfects the liquid storage tank and the collection cylinder passing near the sealing door, ensuring the convenience of replacing the liquid storage tank and discharging the waste liquid in the collection cylinder, and having a good disinfection effect. Description of the Drawings

[0019] Figure 1 is a three-dimensional structural schematic diagram of the present invention;

[0020] Figure 2 is a rear three-dimensional structural schematic diagram of the present invention;

[0021] Figure 3 is a structural schematic diagram of the liquid adding auxiliary box and the culture box of the present invention in an exploded state;

[0022] Figure 4 Schematic cross-sectional structure diagram of the liquid adding auxiliary tank of the present invention;

[0023] Figure 5 Schematic three-dimensional structure diagram of the liquid collection device of the present invention;

[0024] Figure 6 Schematic three-dimensional structure diagram of the rotating seat of the present invention;

[0025] Figure 7 Schematic cross-sectional structure diagram of the rotating seat of the present invention;

[0026] Figure 8 Schematic three-dimensional structure diagram of the liquid storage tank of the present invention;

[0027] Figure 9 Schematic three-dimensional structure diagram of the elastic positioning device of the present invention;

[0028] Figure 10 Schematic three-dimensional structure diagram of the liquid injection device of the present invention;

[0029] Figure 11 Schematic three-dimensional structure diagram of the driving device of the present invention;

[0030] In the figure: 1. Liquid adding auxiliary tank; 2. Culture box; 3. Sealing door; 4. Ultraviolet disinfection lamp; 5. Sealing contact seat; 6. Rotating seat; 7. First placement groove; 8. Second placement groove; 9. Liquid collection device; 91. Collection cylinder; 92. Filter plate; 93. Drain pipe; 10. Limiting groove; 11. Connecting groove; 12. Liquid storage tank; 13. Positioning groove; 14. Elastic positioning device; 141. Sliding sleeve; 142. Positioning rod; 143. Extension piece; 144. First elastic component; 145. Contact block; 15. Limiting column; 16. Liquid injection device; 161. Control valve; 162. Liquid injection pipe; 163. Rotating gear; 164. Rack; 165. Sliding block; 166. Second elastic component; 167. Magnet; 17. Magnetic plate; 18. Rotating shaft; 19. Bearing; 20. Driving device; 201. Driving component; 202. First gear; 203. Second gear; 21. Extrusion ring. Detailed implementation manners

[0031] Next, the technical solutions in the embodiments of the present invention will be clearly and completely described in conjunction with the accompanying drawings in the embodiments of the present invention. Obviously, the described embodiments are only a part of the embodiments of the present invention, rather than all the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by those of ordinary skill in the art without creative efforts shall fall within the protection scope of the present invention.

[0032] Such as Figures 1-11As shown in the figure, the present invention provides an auxiliary device for engineering macrophage culture, including a liquid adding auxiliary box 1 and a culture box 2. The culture box 2 is arranged through one side of the liquid adding auxiliary box 1. A sealed contact seat 5 is connected inside the liquid adding auxiliary box 1, and a rotating seat 6 is connected outside the sealed contact seat 5. By setting the sealed contact seat 5 and the rotating seat 6, the contact parts between the sealed contact seat 5 and the culture box 2 and the rotating seat 6 can ensure that the rotating seat 6 remains in a closed state with the culture box 2 during the rotation process;

[0033] A rotating shaft 18 is connected to the rotating seat 6. A driving device 20 is arranged outside the rotating shaft 18. The driving device 20 is connected inside the liquid adding auxiliary box 1. A first placement groove 7 is opened outside the rotating seat 6, and a second placement groove 8 is opened outside the rotating seat 6. A liquid collection device 9 is connected inside the first placement groove 7, and a liquid storage tank 12 is connected inside the second placement groove 8. A positioning groove 13 is opened on the liquid storage tank 12, and an elastic positioning device 14 is connected inside the positioning groove 13. The elastic positioning device 14 is arranged through the rotating seat 6. A liquid injection device 16 is connected below the liquid storage tank 12. The liquid injection device 16 is located inside the second placement groove 8. A magnetic plate 17 is arranged at the corresponding position of the sealed contact seat 5 for the liquid injection device 16. An extrusion ring 21 is connected to the elastic positioning device 14, and the extrusion ring 21 is connected inside the liquid adding auxiliary box 1.

[0034] A sealed door 3 is arranged outside the liquid adding auxiliary box 1. An ultraviolet disinfection lamp 4 is connected to one side inside the liquid adding auxiliary box 1 outside the sealed door 3. The position of the ultraviolet disinfection lamp 4 corresponds to the positions of the liquid storage tank 12 and the liquid collection device 9. By setting the sealed door 3, the sealed door 3 is used to discharge the waste liquid in the collection cylinder 91 into the liquid adding auxiliary box 1, and the liquid storage tank 12 can be replaced. By setting the ultraviolet disinfection lamp 4, the ultraviolet disinfection lamp 4 uses ultraviolet rays to irradiate and disinfect the liquid storage tank 12 and the collection cylinder 91.

[0035] A limiting column 15 is connected outside the liquid storage tank 12, and a limiting groove 10 is opened inside the second placement groove 8. The limiting column 15 is slidably arranged inside the limiting groove 10. A bearing 19 is sleeved outside the rotating shaft 18, and the bearing 19 is connected inside the liquid adding auxiliary box 1. A liquid outlet pipe is communicated outside the liquid adding auxiliary box 1. By setting the liquid outlet pipe, the waste liquid at the bottom of the liquid adding auxiliary box 1 is discharged. By setting the limiting column 15 and the limiting groove 10, the limiting groove 10 limits the limiting column 15 and the liquid storage tank 12 to ensure that the liquid storage tank 12 can be placed relatively stably inside the second placement groove 8. By setting the rotating shaft 18 and the bearing 19, the bearing 19 limits the rotating shaft 18 and the rotating seat 6 to ensure the smooth and stable rotation of the rotating shaft 18 and the rotating seat 6.

[0036] A connecting groove 11 is opened inside the second placement groove 8, and the liquid injection device 16 is arranged inside the connecting groove 11. The sealed contact seat 5 is set to be arc-shaped. By setting the connecting groove 11, the connecting groove 11 guides and limits part of the action process of the liquid injection device 16.

[0037] The liquid collection device 9 includes a collection cylinder 91, the collection cylinder 91 is connected in the first placement groove 7, a filter plate 92 is connected through the outside of the collection cylinder 91, the position of the filter plate 92 corresponds to the position of the culture box 2, a drain pipe 93 is connected under the collection cylinder 91. By providing the collection cylinder 91, the collection cylinder 91 is used for storing the waste liquid in the culture box 2. By providing the filter plate 92, the filter plate 92 is used for filtering the nutrient solution that needs to be replaced in the culture box 2, so that the nutrient solution can smoothly enter the collection cylinder 91. By providing the drain pipe 93, the drain pipe 93 is used for discharging the waste liquid in the collection cylinder 91 into the liquid addition auxiliary box 1.

[0038] The elastic positioning device 14 includes a sliding sleeve 141, the sliding sleeve 141 is connected through the rotating seat 6, a positioning rod 142 is slidably connected in the sliding sleeve 141, the positioning rod 142 is arranged in the positioning groove 13, an extension piece 143 is connected outside the positioning rod 142, a first elastic component 144 is sleeved outside the positioning rod 142, one end of the first elastic component 144 is connected with the sliding sleeve 141, the other end of the first elastic component 144 is connected with the extension piece 143, the top end of the positioning rod 142 is connected with a contact block 145, and the contact block 145 is arranged under the extrusion ring 21;

[0039] By providing the sliding sleeve 141, the sliding sleeve 141 guides the actions of the positioning rod 142 and the contact block 145, ensuring that the contact block 145 smoothly and stably moves corresponding to the position of the extrusion ring 21, and the positioning rod 142 can smoothly and accurately move corresponding to the positioning groove 13. By providing the positioning rod 142 and the positioning groove 13, when the positioning rod 142 is inserted into the positioning groove 13, it limits the positioning groove 13 and the liquid storage tank 12. By providing the contact block 145 and the extrusion ring 21, when the contact block 145 is located at the position corresponding to the sealing door 3, it separates from the extrusion ring 21, and the extrusion ring 21 can extrude the contact block 145 that rotates away from the sealing door 3, controlling the positioning rod 142 to automatically move downward. By providing the first elastic component 144, after the contact block 145 separates from the extrusion ring 21, the first elastic component 144 can automatically control the positioning rod 142 to move upward and separate from the positioning groove 13.

[0040] The liquid injection device 16 includes a control valve 161, the control valve 161 is arranged under the liquid storage tank 12, a liquid injection pipe 162 is connected under the control valve 161, the liquid injection pipe 162 is communicated with the liquid storage tank 12 through the control valve 161, a rotating gear 163 is arranged outside the control valve 161, and a rack 164 is externally engaged with the rotating gear 163. By providing the control valve 161 and the liquid injection pipe 162, the control valve 161 is used for controlling the on-off of the liquid injection pipe 162, and the liquid injection pipe 162 can inject the nutrient solution in the liquid storage tank 12 into the culture box 2. By providing the rotating gear 163, the opening and closing states of the control valve 161 can be adjusted by using the rotation of the rotating gear 163.

[0041] One end of the rack 164 is connected with a sliding block 165. The sliding block 165 is slidably connected in the connecting groove 11. A second elastic component 166 is connected outside the sliding block 165. The other end of the second elastic component 166 is connected with the connecting groove 11. The other end of the rack 164 is connected with a magnetic block 167. The surfaces of the magnetic block 167 and the magnetic plate 17 facing each other have the same magnetic property. The position of the magnetic block 167 corresponds to the position of the magnetic plate 17.

[0042] By providing the sliding block 165 and the rack 164, the connecting groove 11 guides the actions of the sliding block 165 and the rack 164, ensuring that the rack 164 stably moves relative to the rotating gear 163. By providing the second elastic component 166, after the magnetic block 167 is separated from the magnetic plate 17, the second elastic component 166 automatically controls the rack 164 to extend out of the connecting groove 11. By providing the magnetic block 167 and the magnetic plate 17, when the magnetic block 167 approaches the magnetic plate 17, the magnetic force between the two controls the rack 164 to extend into the connecting groove 11.

[0043] The driving device 20 includes a driving component 201. The driving component 201 is connected in the liquid adding auxiliary tank 1. The output shaft of the driving component 201 is connected with a first gear 202. The first gear 202 is externally meshed with a second gear 203. The second gear 203 is connected to the rotating shaft 18. By providing the driving component 201 and the first gear 202, the driving component 201 is used to control the rotation of the first gear 202. The first gear 202 is an incomplete gear. When the first gear 202 rotates one week, it can control the second gear 203 and the rotating shaft 18 to rotate one quarter of a week.

[0044] A method for using an auxiliary device for engineering macrophage culture includes the following steps:

[0045] When it is necessary to replace the nutrient solution in the culture box 2, directly control the driving component 201 to work. The driving component 201 controls the second gear 203 and the rotating seat 6 to rotate through the first gear 202. At this time, the collection cylinder 91 rotates to a position close to the culture box 2, and at the same time, the filter plate 92 is located in the culture box 2. At this time, the first gear 202 is disengaged from the second gear 203. The nutrient solution in the culture box 2 enters the collection cylinder 91 through the filter plate 92. At this time, the collection of waste liquid is completed. When the contact block 145 contacts the extrusion ring 21, it is extruded. At this time, control the positioning rod 142 to slide downward;

[0046] The positioning rod 142 enters the positioning groove 13 to complete the limit between the liquid storage tank 12 and the rotating base 6. The first gear 202 continues to rotate and meshes with the second gear 203 to control the rotation of the second gear 203 and the rotating base 6. At this time, the collection cylinder 91 is controlled to move away from the culture box 2. The liquid storage tank 12 rotates to the position corresponding to the culture box 2. The magnet 167 moves away from the magnetic plate 17, and the magnetic force between the two disappears. At the same time, the second elastic component 166 controls the movement of the sliding block 165 and the toothed rod 164. The toothed rod 164 controls the rotation of the rotating gear 163 and the control valve 161 through meshing. The control valve 161 opens. At this time, the nutrient solution in the liquid storage tank 12 is injected into the culture box 2 through the control valve 161 and the liquid injection pipe 162 to complete the liquid change process. When the first gear 202 meshes with the second gear 203 again, the rotating base 6 and the liquid storage tank 12 are controlled to rotate at this time. The magnet 167 moves close to the magnetic plate 17, and the magnetic force between the two controls the toothed rod 164 to extend into the connecting groove 11. The toothed rod 164 drives the rotation of the rotating gear 163 and the control valve 161, and the control valve 161 closes.

[0047] Finally, several points should be noted: First, in the description of the present application, it should be noted that unless otherwise specified and limited, the terms "installation", "connection", and "connection" should be understood in a broad sense, which can be mechanical connection or electrical connection, or the communication inside two components, and can be directly connected. "Up", "down", "left", "right", etc. are only used to represent the relative position relationship. When the absolute position of the described object changes, the relative position relationship may change;

[0048] Secondly: In the drawings of the disclosed embodiments of the present invention, only the structures related to the disclosed embodiments are involved. Other structures can refer to the general design. Without conflict, the same embodiment and different embodiments of the present invention can be combined with each other;

[0049] Finally: The above are only the preferred embodiments of the present invention and are not used to limit the present invention. Any modifications, equivalent replacements, improvements, etc. made within the spirit and principle of the present invention shall be included in the protection scope of the present invention.

Claims

1. An auxiliary device for culturing engineered macrophages, comprising a liquid-adding auxiliary box (1) and a culture box (2), characterized in that: The culture box (2) is arranged on one side of the liquid adding auxiliary box (1); the liquid adding auxiliary box (1) is connected with a sealing contact seat (5); the sealing contact seat (5) is connected with a rotating seat (6) on the outside; the rotating seat (6) is connected with a rotating shaft (18); the rotating shaft (18) is provided with a driving device (20) on the outside; the driving device (20) is connected to the liquid adding auxiliary box (1); the rotating seat (6) is provided with a first placement groove (7); the rotating seat (6) is provided with a second placement groove (8); the first placement groove (7) is connected with a liquid collecting device (9); the second placement groove ( A liquid storage tank (12) is connected to the inner portion of the sealing contact seat (5), a positioning groove (13) is provided on the liquid storage tank (12), an elastic positioning device (14) is connected to the positioning groove (13), the elastic positioning device (14) is arranged on the rotating seat (6), a liquid injection device (16) is connected to the lower portion of the liquid storage tank (12), the liquid injection device (16) is arranged in the second placement groove (8), a magnetic plate (17) is arranged at a position corresponding to the liquid injection device (16) in the sealing contact seat (5), an extrusion ring (21) is connected to the elastic positioning device (14), and the extrusion ring (21) is connected to the liquid adding auxiliary box (1).

2. The auxiliary device for culturing engineered macrophages according to claim 1, characterized in that: The liquid adding auxiliary box (1) is provided with a sealed door (3) outside, and a side of the sealed door (3) located inside the liquid adding auxiliary box (1) is connected to an ultraviolet disinfection lamp (4), and the position of the ultraviolet disinfection lamp (4) corresponds to the position of the liquid storage box (12) and the liquid collecting device (9).

3. The auxiliary device for culturing engineered macrophages according to claim 1, characterized in that: The liquid storage box (12) is externally connected to a limiting column (15), the second placement groove (8) is provided with a limiting column (15), the limiting column (15) is slidably arranged in the limiting groove (10), the outer sleeve of the rotating shaft (18) is provided with a bearing (19), the bearing (19) is connected to the liquid adding auxiliary box (1), and the liquid adding auxiliary box (1) is externally connected to a liquid outlet pipe.

4. The auxiliary device for culturing engineered macrophages according to claim 1, characterized in that: A connecting groove (11) is provided in the second placement groove (8), the liquid injection device (16) is arranged in the connecting groove (11), and the sealing contact seat (5) is arranged in an arc shape.

5. The auxiliary device for culturing engineered macrophages according to claim 1, characterized in that: The liquid collecting device (9) comprises a collecting tube (91), wherein the collecting tube (91) is connected to the first placement groove (7), a filter plate (92) is connected to the outside of the collecting tube (91), the position of the filter plate (92) corresponds to the position of the culture box (2), and a drainage pipe (93) is connected below the collecting tube (91).

6. The auxiliary device for culturing engineered macrophages according to claim 1, characterized in that: The elastic positioning device (14) comprises a sliding sleeve (141), wherein the sliding sleeve (141) is connected to the rotating seat (6), a positioning rod (142) is slidably connected inside the sliding sleeve (141), the positioning rod (142) is arranged in the positioning groove (13), an extension piece (143) is connected to the outside of the positioning rod (142), a first elastic component (144) is provided on the outer sleeve of the positioning rod (142), one end of the first elastic component (144) is connected to the sliding sleeve (141), and the other end of the first elastic component (144) is connected to the extension piece (143), and a contact block (145) is connected to the top end of the positioning rod (142), and the contact block (145) is arranged under the extrusion ring (21).

7. The auxiliary device for culturing engineered macrophages according to claim 1, characterized in that: The liquid injection device (16) comprises a control valve (161), the control valve (161) being arranged below the liquid storage tank (12), the control valve (161) being connected to a liquid injection pipe (162), the liquid injection pipe (162) being connected to the liquid storage tank (12) through the control valve (161), the control valve (161) being provided with a rotating gear (163) outside, the rotating gear (163) being meshed with a gear rod (164) outside.

8. The auxiliary device for culturing engineered macrophages according to claim 7, characterized in that: One end of the gear rod (164) is connected to a sliding block (165), and the sliding block (165) is slidably connected in the connecting groove (11). The sliding block (165) is externally connected to a second elastic component (166), and the other end of the second elastic component (166) is connected to the connecting groove (11). The other end of the gear rod (164) is connected to a magnetic block (167), and a side of the magnetic block (167) close to the magnetic plate (17) has the same magnetism, and the position of the magnetic block (167) corresponds to the position of the magnetic plate (17).

9. The auxiliary device for culturing engineered macrophages according to claim 1, characterized in that: The driving device (20) comprises a driving component (201), wherein the driving component (201) is connected to the liquid adding auxiliary box (1), the output shaft of the driving component (201) is connected to a first gear (202), the first gear (202) is externally meshed with a second gear (203), and the second gear (203) is connected to the outside of the rotating shaft (18).

10. An auxiliary device for culturing engineered macrophages according to any one of claims 1 to 9, characterized in that: The method for using the auxiliary device comprises the following steps: When the nutrient solution in the culture box (2) needs to be replaced, the drive assembly (201) is directly controlled to work, and the drive assembly (201) controls the second gear (203) and the rotating seat (6) to rotate through the first gear (202). At this time, the collecting tube (91) rotates to a position close to the culture box (2), and the filter plate (92) is located in the culture box (2). At this time, the first gear (202) and the second gear (203) are disengaged, and the nutrient solution in the culture box (2) enters the collecting tube (91) through the filter plate (92). At this time, the collection of waste liquid is completed, and when the contact block (145) contacts and is squeezed by the squeezing ring (21), the positioning rod (142) is controlled to slide downward; The positioning rod (142) enters the positioning groove (13) to complete the position limiting between the liquid storage tank (12) and the rotating seat (6). The first gear (202) continues to rotate and meshes with the second gear (203), controlling the second gear (203) and the rotating seat (6) to rotate. At this time, the collecting cylinder (91) is controlled to rotate away from the culture box (2), and the liquid storage tank (12) rotates to a position corresponding to the culture box (2). The magnetic block (167) moves away from the magnetic plate (17), and the magnetic force between the two disappears. At the same time, the second elastic component (166) controls the movement of the sliding block (165) and the gear rod (164), and the gear rod (164) controls the rotating gear by meshing. The wheel (163) and the control valve (161) rotate, and the control valve (161) opens. At this time, the nutrient solution in the liquid storage tank (12) is injected into the culture box (2) through the control valve (161) and the injection pipe (162), completing the liquid replacement process. When the first gear (202) is meshed with the second gear (203) again, the rotating seat (6) and the liquid storage tank (12) are controlled to rotate, and the magnetic block (167) moves close to the magnetic plate (17). The magnetic force between the two controls the gear rod (164) to extend into the connecting groove (11), and the gear rod (164) drives the rotating gear (163) and the control valve (161) to rotate, and the control valve (161) is closed.

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