An edible and medicinal fungal mycelium fiber-based sensing chip embedded paper and its preparation method and application

Abstract

The object of the present invention is to provide an edible and medicinal fungus mycelium fiber-based induction chip embedded paper, its preparation method and application, which relate to the technical field of microbial fermentation. The preparation method of the edible and medicinal fungus mycelium fiber-based induction chip embedded paper includes the following steps: culturing the aerial mycelium of the edible and medicinal fungus, drying the aerial mycelium of the edible and medicinal fungus with an induction chip, preparing the mycelium fiber of the edible and medicinal fungus, preparing the mycelium fiber pulp of the edible and medicinal fungus, and preparing the edible and medicinal fungus mycelium fiber paper embedded with an induction chip. The prepared mycelium fiber-based induction chip embedded paper has good strength and certain waterproof performance, and can be used as packaging paper in multiple fields such as intelligent packaging, logistics tracking, and medical monitoring.

Description

Technical Field

[0001] The present invention relates to the technical field of microbial fermentation, and particularly relates to an edible and medicinal mushroom mycelium fiber-based induction chip embedded paper, a preparation method thereof, and an application thereof. Background Art

[0002] As a widely used material, paper plays an irreplaceable role in society. Traditional paper is mainly made from wood fibers through processes such as strong alkali and strong acid treatment, followed by bleaching, papermaking, drying, and post-treatment; it has problems such as high consumption of wood resources and environmental pollution during the production process. Mycelium fiber, as a new type of renewable resource, has advantages such as scalable cultivation, fast growth rate, easy degradation after use, and environmental friendliness, and has received extensive attention globally.

[0003] Mycelium fiber mainly comes from edible and medicinal mushrooms and is a renewable resource. Compared with wood, the cultivation cycle of edible and medicinal mushrooms is short, and the cultivation cycle of the mycelium of edible and medicinal mushrooms is even shorter. Preparing paper with edible and medicinal mushroom mycelium fiber as the main raw material helps reduce the dependence of the paper industry on forests and does not require alkali or acid treatment. Mycelium fiber paper has good physical properties such as flexibility, strength, and stiffness. At the same time, by treating the mycelium fiber and optimizing the paper preparation process, it is possible to adjust the surface smoothness, gloss, and other properties of the paper and expand the application fields of the paper.

[0004] At present, the anti-counterfeiting and traceability of regular products have become hot issues of concern to production enterprises and consumers. Given the extensive application basis of paper in fields such as packaging, printing, and labeling, the present invention combines mycelium fiber paper with an induction chip, which can provide a new solution for enterprise product management and anti-counterfeiting. At present, most existing products use industrial glue to bond the induction chip to the paper, which not only affects the performance of the chip but also causes environmental pollution. The edible and medicinal mushroom mycelium fiber-based induction chip embedded paper of the present invention is a new type of intelligent and environmentally friendly paper, which has advantages such as renewable, degradable, and pollution-free; at the same time, the induction chip can realize functions such as anti-counterfeiting inspection, traceability, and counting. It is embedded inside the mycelium fiber paper, increasing the concealment of the chip and endowing the paper with more functions and application values; in addition, based on the unique physical properties of the mycelium fiber paper, even after multiple folds and rubs, it will not affect the induction function of the embedded chip, ensuring the stable function of the chip. Therefore, the present invention can provide an effective method for enterprises in aspects such as product anti-counterfeiting inspection, traceability, and counting, reducing the technical, human, and time costs of enterprises, and not only having broad market application prospects but also having good ecological benefits. Summary of the Invention

[0005] The present invention provides an edible and medicinal fungus mycelium fiber-based induction chip embedded paper, its preparation method and application, pioneering a green paper preparation process that does not use strong alkalis and strong acids and is environmentally friendly; the prepared edible and medicinal fungus mycelium fiber-based induction chip embedded paper is a new type of environmentally friendly paper with the advantages of being renewable and degradable; it realizes the natural embedding of the induction chip during the forming process of the edible and medicinal fungus mycelium fiber-based induction chip embedded paper without affecting the chip function. At the same time, the edible and medicinal fungus mycelium fiber-based induction chip embedded paper has good flexibility and elasticity, and can play a role in buffering and protecting the induction chip under external forces such as folding and kneading, ensuring the functional stability of the induction chip, and can realize functions such as forgery detection, traceability, and counting.

[0006] According to one aspect of the present invention: a preparation method of an edible and medicinal fungus mycelium fiber-based induction chip embedded paper is provided, and the method includes the following steps:

[0007] Step 1, culturing the aerial mycelium of edible and medicinal fungi:

[0008] Inoculate the strain of edible and medicinal fungi into a solid medium, with an inoculation amount of 5% - 10%, and place it in a culture room for culturing to obtain the aerial mycelium of edible and medicinal fungi;

[0009] Step 2, drying the aerial mycelium of edible and medicinal fungi with an induction chip:

[0010] Place a surface-clean induction chip in the aerial mycelium of edible and medicinal fungi, culture for 1 - 3 days to obtain the aerial mycelium of edible and medicinal fungi with an induction chip, take it out and perform a drying treatment to obtain the dried aerial mycelium of edible and medicinal fungi with an induction chip;

[0011] Step 3, preparing the mycelium fiber of edible and medicinal fungi:

[0012] Take out the induction chip covered with the aerial mycelium of edible and medicinal fungi from the dried aerial mycelium of edible and medicinal fungi with an induction chip for standby, and then grind the remaining dried aerial mycelium of edible and medicinal fungi with water to make a mycelium fiber liquid of edible and medicinal fungi. After centrifuging multiple times, obtain the mycelium fiber of edible and medicinal fungi;

[0013] Step 4, preparing the mycelium fiber pulp of edible and medicinal fungi:

[0014] Mix the mycelium fiber of edible and medicinal fungi with water to prepare a mycelium fiber suspension of edible and medicinal fungi, then add sodium carboxymethylcellulose to obtain suspension 1, stir with a magnetic stirrer, then add cationic starch to obtain suspension 2, and then stir with a magnetic stirrer to obtain the mycelium fiber pulp of edible and medicinal fungi;

[0015] Step 5, preparing the edible and medicinal fungus mycelium fiber-based induction chip embedded paper:

[0016] The edible and medicinal fungus mycelial fiber pulp is added with water to prepare an edible and medicinal fungus pulp suspension, which is filtered by a vacuum filter to obtain two edible and medicinal fungus mycelial fiber cakes. One of the edible and medicinal fungus mycelial fiber cakes is laid flat in a mold. After placing the induction chip covered with the edible and medicinal fungus aerial mycelium in step 3, another edible and medicinal fungus mycelial fiber cake is placed to form an edible and medicinal fungus mycelial fiber cake embedded with the induction chip. It is placed in a paper former, formed, dehydrated and dried to obtain an edible and medicinal fungus mycelial fiber-based induction chip-embedded paper.

[0017] Preferably, the edible and medicinal fungus in step 1 is selected from any one of Ganoderma lucidum, Phellinus igniarius, Lentinula edodes, Auricularia auricula, Hericium erinaceus, Grifola frondosa, Poria cocos.

[0018] Preferably, the preparation method of the solid medium in step 1 is: loading the solid medium raw materials into a cultivation bag, making small holes in the center of the bag body of the cultivation bag; sterilizing at 115-121 °C for 20-120 min; waiting for the solid medium to cool to 25-30 °C to obtain the solid medium.

[0019] Preferably, the solid medium in step 1 includes solid medium raw materials and water, and the solid medium raw materials include: 70%-78% of sawdust, 20%-26% of wheat bran, 1%-2% of gypsum, and 1%-2% of sugar.

[0020] Preferably, after step 1, the method further includes: screening the edible and medicinal fungus aerial mycelium with a white color, no abnormal discoloration, and uniform and dense growth by the naked eye observation method; screening the edible and medicinal fungus aerial mycelium with thick morphology and a hyphal diameter of not less than 1 μm by the scanning electron microscope observation method.

[0021] Preferably, the specific method of the drying treatment in step 2 is: putting the edible and medicinal fungus aerial mycelium with the induction chip into a constant temperature blast drying oven, at a temperature of 45-65 °C, and drying for 5-10 h.

[0022] Preferably, after step 2, the method further includes: screening the dried edible and medicinal fungus aerial mycelium with the induction chip by the vacuum drying method, and the water content does not exceed 4%.

[0023] Preferably, the specific method for preparing the edible and medicinal fungal mycelial fiber in step 3 is as follows: Take out the induction chip covered with the aerial mycelium of the edible and medicinal fungus from the dried aerial mycelium of the edible and medicinal fungus with an induction chip and set it aside. Then, grind the remaining dried aerial mycelium of the edible and medicinal fungus with water to make an edible and medicinal fungal mycelial fiber solution. Place the edible and medicinal fungal mycelial fiber solution in a water bath at 45 - 65 °C and stir it at a speed of 200 - 300 r / min for 60 - 120 min. Then, place the edible and medicinal fungal mycelial fiber solution in a centrifuge and centrifuge it at 3500 - 4500 r / min for 10 - 15 min. Rinse the centrifuged precipitate with pure water and then centrifuge it at 3500 - 4500 r / min for 10 - 15 min. The obtained precipitate is the edible and medicinal fungal mycelial fiber.

[0024] Preferably, the specific method for grinding the remaining dried aerial mycelium of the edible and medicinal fungus with water in step 3 is as follows: Put the dried aerial mycelium of the edible and medicinal fungus into a pulper, add pure water to obtain an aerial mycelium suspension of the edible and medicinal fungus, so that the concentration of the aerial mycelium of the edible and medicinal fungus in the aerial mycelium suspension of the edible and medicinal fungus is 40 - 60 g / 100 mL. Set the gap distance between the grinding discs to 1 - 5 mm, the power of the flying knife rotor to 1.1 kW, and the speed of the flying knife to 1430 - 1470 r / min. Grind the aerial mycelium suspension of the edible and medicinal fungus for 5 - 15 min to obtain an edible and medicinal fungal mycelial fiber solution.

[0025] Preferably, after step 3, the method further includes: Screening the edible and medicinal fungal mycelial fiber with a laser confocal fluorescence microscope to have a length of not less than 20 μm and a width of not more than 1 μm.

[0026] Preferably, the specific method for preparing the edible and medicinal fungal mycelial fiber pulp in step 4 is as follows: Prepare an edible and medicinal fungal mycelial fiber suspension by adding water to the edible and medicinal fungal mycelial fiber, add sodium carboxymethylcellulose to obtain suspension 1, stir it with a magnetic stirrer, and stir suspension 1 at 45 - 65 °C at a speed of 150 - 200 r / min for 15 - 45 min. Then add cationic starch to obtain suspension 2, and then stir it with a magnetic stirrer. Stir suspension 2 at 45 - 65 °C at a speed of 150 - 200 r / min for 15 - 45 min to obtain the edible and medicinal fungal mycelial fiber pulp.

[0027] Preferably, the concentration of the edible and medicinal fungal mycelial fiber in the edible and medicinal fungal mycelial fiber suspension in step 4 is 30 - 60 g / 100 mL, the concentration of sodium carboxymethylcellulose in suspension 1 is 0.2 - 1.2 g / 100 mL, and the concentration of cationic starch in suspension 2 is 0.4 - 1.4 g / 100 mL.

[0028] Preferably, after step 4, the method further includes: screening with a potentiometer for the surface charge of the edible and medicinal fungal mycelial fiber pulp to be not less than 8 mV, and screening with a particle size analyzer for the average particle size of the edible and medicinal fungal mycelial fiber pulp to be not less than 1 mm.

[0029] Preferably, in step 5, the concentration of the edible and medicinal fungal mycelial fibers in the edible and medicinal fungal pulp suspension is 0.7 - 1.4 g / 100 mL; the grammage of the edible and medicinal fungal mycelial fiber filter cake embedded with the induction chip in step 5 is 50 - 100 g / m2; the specific conditions for dehydration and drying in step 5 are: heating power 10 - 20 kw, drying temperature 40 - 60 °C, and drying time 12 - 24 h.

[0030] Preferably, after step 5, the method further includes: screening with a contact angle measuring instrument for the contact angle between the edible and medicinal fungal mycelial fiber-based induction chip embedded paper and water to be not less than 79°; screening with a folding endurance tester for the edible and medicinal fungal mycelial fiber-based induction chip embedded paper not to affect the induction function of the induction chip after 100 folds; screening with an electronic universal tester for the tensile strength of the edible and medicinal fungal mycelial fiber-based induction chip embedded paper to be not less than 180 MPa; screening with a smoothness tester for the surface smoothness of the edible and medicinal fungal mycelial fiber-based induction chip embedded paper to be not less than 600 s; screening with a stiffness tester for the stiffness of the edible and medicinal fungal mycelial fiber-based induction chip embedded paper to be not less than 0.1 mNm; screening with a glossmeter for the gloss of the edible and medicinal fungal mycelial fiber-based induction chip embedded paper to be not less than 70%.

[0031] According to another aspect of the present invention, there is also provided a paper prepared by the preparation method of the edible and medicinal fungal mycelial fiber-based induction chip embedded paper.

[0032] According to another aspect of the present invention, there is also provided the application of the edible and medicinal fungal mycelial fiber-based induction chip embedded paper as a packaging paper.

[0033] The beneficial effects of the present invention are: The method for preparing paper with edible and medicinal fungal mycelial fibers as raw materials helps to reduce the dependence on traditional wood fibers and meets the requirements of environmental protection and sustainable development; The edible and medicinal fungal mycelial fiber-based induction chip embedded paper is innovatively developed, so it has good strength and certain waterproof performance, ensuring the stability and reliability in the edible and medicinal fungal mycelial fiber-based induction chip embedded paper. The edible and medicinal fungal mycelial fiber-based induction chip embedded paper prepared by the present invention can be applied to multiple fields such as intelligent packaging, logistics tracking, and medical monitoring, and has broad market prospects. BRIEF DESCRIPTION OF THE DRAWINGS

[0034] Figure 1 It is a specific implementation flowchart of the present invention.

[0035] Figure 2 It is the apparent morphological diagram of the aerial hyphae of Ganoderma lucidum.

[0036] Figure 3 It is the morphological diagram of the aerial hyphae of Ganoderma lucidum observed under a scanning electron microscope.

[0037] Figure 4 It is the schematic diagram of the sensing chip.

[0038] Figure 5 It is the schematic diagram of the surface of the sensing chip being surrounded and intertwined by the aerial hyphae of Ganoderma lucidum.

[0039] Figure 6 It is the morphological diagram of Ganoderma lucidum fungal fibers observed under a laser confocal fluorescence microscope.

[0040] Figure 7 It is the morphological diagram of the Ganoderma lucidum fungal fiber-based sensing chip embedded paper before molding when placed in the mold.

[0041] Figure 8 It is the morphological diagram of the Ganoderma lucidum fungal fiber-based sensing chip embedded paper before molding.

[0042] Figure 9 It is the morphological diagram of the Ganoderma lucidum fungal fiber-based sensing chip embedded paper after molding. Detailed implementation manners

[0043] Definition:

[0044] The term "edible and medicinal mushroom fungal fiber" used in this article refers to: a type of dietary fiber present in the cell wall of edible and medicinal mushrooms, mainly composed of polysaccharide substances, including β-glucan, chitin, cellulose, etc.

[0045] The term "aerial hyphae" used in this article refers to: a type of mycelium of edible and medicinal mushrooms, which is the mycelium that extends into the space after the growth and development of the mycelium in the culture medium. On solid culture media, aerial hyphae are visible to the naked eye, often covering the surface of the culture medium, presenting a fluffy appearance. Aerial hyphae have multiple important physiological functions. On the one hand, it can further extend and expand to explore new nutrient sources and find a suitable growth environment; on the other hand, when the aerial hyphae develop to a certain stage, they will differentiate into reproductive organs, such as producing sporangia, conidiophores, etc., for producing various asexual or sexual spores to achieve the reproduction and spread of edible and medicinal mushrooms. During the artificial cultivation of edible and medicinal mushrooms, the growth status of aerial hyphae is one of the important indicators for judging whether the cultivation conditions are suitable. For example, if the aerial hyphae grow too vigorously, it may affect the formation and development of fruiting bodies, which may be related to factors such as humidity, temperature, and ventilation in the cultivation environment. On the contrary, if the aerial hyphae grow poorly, it may mean insufficient nutrition or unsuitable environmental conditions, and the cultivation conditions need to be adjusted.

[0046] The term "solid culture medium" as used herein refers to a solid substance that provides nutrition and a habitat for the growth, development, and reproduction of edible and medicinal fungi. Its components include:

[0047] (1) Main nutrient sources, including carbon sources (such as glucose, sucrose, starch, sawdust, corn cobs, etc., which provide energy for the growth of edible and medicinal fungi and synthesize the carbon skeleton of cell substances), nitrogen sources (such as peptone, yeast powder, soybean cake powder, bran, corn flour, etc., which meet the nitrogen needs of fungi and are used to synthesize nitrogen-containing biological molecules such as proteins and nucleic acids).

[0048] (2) Inorganic salts: These provide the mineral elements necessary for the growth of edible and medicinal fungi, such as phosphorus, potassium, magnesium, and calcium. These elements are generally met by adding inorganic salts such as potassium dihydrogen phosphate, magnesium sulfate, and calcium sulfate. These elements play an important role in cell metabolism and enzyme activity regulation.

[0049] (3) Growth factors, including vitamins, amino acids, nucleic acids, and other organic substances that are essential for the growth of edible and medicinal fungi but cannot be synthesized by themselves or are synthesized in insufficient quantities. For example, yeast extract is rich in growth factors such as B vitamins, which can promote the growth and development of fungi.

[0050] (4) Coagulant, the most commonly used is agar, which can keep the culture medium solid at room temperature and provide a support structure for the growth of fungal hyphae.

[0051] Embodiment 1 of the present invention:

[0052] Step 1, cultivation of aerial mycelium of Ganoderma lucidum:

[0053] 936 g of sawdust, 240 g of bran, 12 g of gypsum, and 12 g of sugar are used as raw materials for a solid culture medium, which are stirred evenly, and 1500 g of purified water is added to obtain a solid culture medium, wherein the water content of the solid culture medium is 55%; the solid culture medium is placed in a cultivation bag, which may be a cultivation plastic bag, and a small hole is punched in the center of the bag body; the cultivation bag is then sterilized at 121° C. for 60 minutes and cooled to 25° C. to obtain a solid culture medium; Ganoderma lucidum seeds are inoculated into the solid culture medium in an ultra-clean workbench with an inoculum amount of 5%; the solid culture medium is placed in a culture room at a temperature of 28° C. and a humidity of 70% for 10 days to form white, fluffy, fuzzy, and radially edged Ganoderma lucidum aerial mycelium; and Ganoderma lucidum aerial mycelium that is white in color, free of abnormal discoloration, and uniformly and densely grown is screened by naked eye observation, such as Figure 2 As shown in the figure, scanning electron microscopy was used to screen Ganoderma lucidum aerial hyphae with a thick morphology and a hypha diameter of not less than 1 μm. Figure 3 shown.

[0054] Step 2: Drying of Ganoderma lucidum aerial mycelium with sensor chip:

[0055] Place a circular induction chip with a clean surface, a diameter of 1 cm, and a thickness of 0.3 mm in the aerial hyphae of Ganoderma lucidum growing on a solid medium obtained in Step 1. The induction chip is as Figure 4 shown. After 2 days of cultivation, the surface of the induction chip is surrounded and intertwined by the aerial hyphae of Ganoderma lucidum, as Figure 5 shown, to obtain the aerial hyphae of Ganoderma lucidum with the induction chip; peel the aerial hyphae of Ganoderma lucidum with the induction chip from the solid medium and put it into a constant temperature blast drying oven for drying treatment at a temperature of 45 °C for 6 h to obtain the dried aerial hyphae of Ganoderma lucidum with the induction chip; use the vacuum drying method to screen that the moisture content of the dried aerial hyphae of Ganoderma lucidum with the induction chip does not exceed 4%.

[0056] Step 3, preparation of Ganoderma lucidum mycelial fiber:

[0057] Step 3: The dried aerial hyphae of Ganoderma lucidum with the induction chip obtained in Step 2 can be regarded as two parts: one part is the induction chip covered with the aerial hyphae of Ganoderma lucidum on the surface, and the other part is the dried aerial hyphae of Ganoderma lucidum. First, take out the induction chip covered with the aerial hyphae of Ganoderma lucidum on the surface for standby, and then put 120 g of dried aerial hyphae of Ganoderma lucidum into a pulper and add pure water to obtain a suspension of aerial hyphae of Ganoderma lucidum, so that the concentration of the aerial hyphae of Ganoderma lucidum in the suspension of aerial hyphae of Ganoderma lucidum is 60 g / 100 mL. Set the gap distance between the grinding discs to 1 mm, the power of the flying knife rotor to 1.1 kW, and the rotational speed of the flying knife to 1470 r / min, and grind the aerial hyphae of Ganoderma lucidum for 5 min to obtain a Ganoderma lucidum mycelial fiber liquid; place the Ganoderma lucidum mycelial fiber liquid in a water bath at 65 °C and stir it at a rotational speed of 200 r / min for 120 min; then, place the Ganoderma lucidum mycelial fiber liquid in a centrifuge and centrifuge it at 4500 r / min for 15 min; rinse the centrifuged precipitate with pure water and then centrifuge it at 4500 r / min for 15 min. The obtained precipitate is Ganoderma lucidum mycelial fiber; use a laser confocal fluorescence microscope to screen that the length of the Ganoderma lucidum mycelial fiber is not less than 20 μm and the width is not more than 1 μm, as Figure 6 shown.

[0058] Step 4, preparation of Ganoderma lucidum mycelial fiber pulp:

[0059] For the 108 g of Ganoderma lucidum mycelial fiber obtained in Step 3, prepare a Ganoderma lucidum mycelial fiber suspension with a concentration of 30 g / 100 mL using 360 mL of purified water; add sodium carboxymethylcellulose to the Ganoderma lucidum mycelial fiber suspension to obtain Suspension 1, such that the concentration of sodium carboxymethylcellulose in Suspension 1 is 0.2 g / 100 mL, and use a magnetic stirrer to stir Suspension 1 at 65 °C at a rotation speed of 150 r / min for 30 min; then, add cationic starch to Suspension 1 to obtain Suspension 2, such that the concentration of cationic starch in Suspension 2 is 0.5 g / 100 mL, and use a magnetic stirrer to stir Suspension 2 at 65 °C at a rotation speed of 150 r / min for 45 min to obtain Ganoderma lucidum mycelial fiber pulp; use a potentiometer to screen the surface charge of the Ganoderma lucidum mycelial fiber pulp to be not less than 8 mV; use a particle size analyzer to screen the average particle size of the Ganoderma lucidum mycelial fiber pulp to be not less than 1 mm.

[0060] Step 5, Preparation of Ganoderma lucidum mycelial fiber-based inductive chip-embedded paper:

[0061] Take 360 mL of the Ganoderma lucidum mycelial fiber pulp obtained in Step 4 and prepare a Ganoderma lucidum mycelial fiber pulp suspension with 7200 mL of purified water, with the concentration of Ganoderma lucidum mycelial fiber in the Ganoderma lucidum mycelial fiber pulp suspension being 1.5 g / 100 mL. Divide the Ganoderma lucidum mycelial fiber pulp suspension into two portions with a volume ratio of 1:1, and use a vacuum filter to filter the two portions of the Ganoderma lucidum mycelial fiber pulp suspension twice to obtain two Ganoderma lucidum mycelial fiber filter cakes; lay one of the Ganoderma lucidum mycelial fiber filter cakes flat in a square mold with a side length of 20 cm, and use a scraper to level the surface of the Ganoderma lucidum mycelial fiber filter cake; then, place the spare inductive chip covered with Ganoderma lucidum aerial mycelium mentioned in Step 3 in the center of the Ganoderma lucidum mycelial fiber filter cake, and then place the other Ganoderma lucidum mycelial fiber filter cake on top of the first leveled Ganoderma lucidum mycelial fiber filter cake, and use a scraper to level the surface of the Ganoderma lucidum mycelial fiber filter cake; the total grammage of the two overlapping Ganoderma lucidum mycelial fiber filter cakes is 70 g / m 2 ; Remove the mold, and place the overlapping Ganoderma lucidum mycelial fiber filter cakes in a sheet former. As Figure 7 shown, set the heating power to 10 kw, the drying temperature to 45 °C, and the drying time to 12 h to obtain Ganoderma lucidum mycelial fiber-based inductive chip-embedded paper. As Figure 9As shown; a contact angle measuring instrument is used to screen that the contact angle between the Ganoderma lucidum fungal fiber-based induction chip embedded paper and water is not less than 79°; a folding endurance tester is used to screen that the Ganoderma lucidum fungal fiber-based induction chip embedded paper will not affect the induction function of the induction chip after 100 folds; an electronic universal tester is used to screen that the tensile strength of the Ganoderma lucidum fungal fiber-based induction chip embedded paper is not less than 180 MPa; a smoothness tester is used to screen that the surface smoothness of the Ganoderma lucidum fungal fiber-based induction chip embedded paper is not less than 600 s; a stiffness tester is used to screen that the stiffness of the Ganoderma lucidum fungal fiber-based induction chip embedded paper is not less than 0.1 mNm; a glossmeter is used to screen that the gloss of the Ganoderma lucidum fungal fiber-based induction chip embedded paper is not less than 70%.

[0062] Preparation mechanism of edible and medicinal mushroom mycelium fiber-based induction chip embedded paper: A large amount of β-glucan is contained in edible and medicinal mushroom mycelium fiber. β-glucan is a polysaccharide formed by connecting glucose monomers through β-1,4 glycosidic bonds, and its molecular structure contains a large number of hydroxyl (-OH) groups. Sodium carboxymethyl cellulose is a water-soluble polymer compound, and its molecular chain has carboxyl (-COOH) and hydroxyl (-OH). In the pulp mainly composed of edible and medicinal mushroom mycelium fiber, hydrogen bond interaction, electrostatic interaction, etc. can form between the hydroxyl groups on the β-glucan molecules in the edible and medicinal mushroom mycelium fiber and the carboxyl or hydroxyl groups on the sodium carboxymethyl cellulose molecules, and hydrophobic interaction also occurs in the hydrophobic regions of the two molecules; that is, the long-chain structure of sodium carboxymethyl cellulose can play a "bridge" role between the edible and medicinal mushroom mycelium fibers, further enhancing the interaction between the edible and medicinal mushroom mycelium fibers, thereby improving the physical strength properties such as the tensile strength and folding endurance of the paper, and can improve the uniformity of the edible and medicinal mushroom mycelium fiber-based induction chip embedded paper. In addition, in the edible and medicinal mushroom mycelium fiber pulp, the surface of the edible and medicinal mushroom mycelium fiber usually carries a negative charge, while cationic starch carries a positive charge. Based on the principle of electrostatic attraction, cationic starch can adsorb on the surface of the edible and medicinal mushroom mycelium fiber, fill the gaps between the edible and medicinal mushroom mycelium fibers, increase the contact area between the edible and medicinal mushroom mycelium fibers, and promote the combination between the edible and medicinal mushroom mycelium fibers; at the same time, the hydroxyl groups on the cationic starch molecular chain can also form hydrogen bonds with the hydroxyl groups on the surface of the edible and medicinal mushroom mycelium fiber, enhancing the binding force between the edible and medicinal mushroom mycelium fibers and further improving the strength of the edible and medicinal mushroom mycelium fiber-based paper. During the pulping process, cationic starch adsorbs on the surface of the edible and medicinal mushroom mycelium fiber, changes the charge distribution on the surface of the edible and medicinal mushroom mycelium fiber, reduces the electrostatic repulsion between the edible and medicinal mushroom mycelium fibers, enables the edible and medicinal mushroom mycelium fibers to be more evenly dispersed in the pulp; and to a certain extent can hinder the rapid sedimentation of the edible and medicinal mushroom mycelium fibers, prolong the suspension time of the edible and medicinal mushroom mycelium fibers in the pulp, is conducive to the uniform distribution of the edible and medicinal mushroom mycelium fibers, and then improves the uniformity of the paper.

[0063] After the above specific implementation steps, the Ganoderma lucidum mycelium fiber-based induction chip embedded paper is processed, and its apparent morphology is as Figure 6 shown.

[0064] According to another aspect of the present invention, there is also provided a paper prepared by the method of the Ganoderma lucidum mycelium fiber-based induction chip embedded paper.

[0065] According to another aspect of the present invention, there is also provided the application of the Ganoderma lucidum mycelium fiber-based induction chip embedded paper as packaging paper.

[0066] Example 2 of the present invention:

[0067] The main difference from Example 1 is that in Step 1, the composition of the solid culture medium raw materials is different: specifically, it is 888 g of wood chips, 264 g of wheat bran, 12 g of gypsum, and 12 g of sugar, that is, 74% of wood chips, 22% of wheat bran, 1% of gypsum, and 1% of sugar. The other operation steps are the same as those in Example 1.

[0068] Example 3 of the present invention:

[0069] The main difference from Example 1 is that in Step 1, the composition of the solid culture medium raw materials is different: specifically, it is 864 g of wood chips, 264 g of wheat bran, 24 g of gypsum, and 24 g of sugar, that is, 72% of wood chips, 26% of wheat bran, 1% of gypsum, and 1% of sugar. The other operation steps are the same as those in Example 1.

[0070] Example 4 of the present invention:

[0071] The main difference from Example 1 is that in Step 1, the inoculation amount of Ganoderma lucidum strains is 7%, and the other operation steps are the same as those in Example 1.

[0072] Example 5 of the present invention:

[0073] The main difference from Example 1 is that in Step 1, the inoculation amount of Ganoderma lucidum strains is 9%, and the other operation steps are the same as those in Example 1.

[0074] Effect test:

[0075] Determination of parameters of Ganoderma lucidum mycelium fiber-based induction chip embedded paper:

[0076] The parameters of the Ganoderma lucidum mycelium fiber-based induction chip embedded paper prepared through Examples 1 - 5 were measured. A contact angle measuring instrument was used to measure the contact angle between the Ganoderma lucidum mycelium fiber-based induction chip embedded paper and water. A folding endurance tester was used to measure the influence of 100 folds of the Ganoderma lucidum mycelium fiber-based induction chip embedded paper on the induction function of the induction chip. An electronic universal tester was used to measure the tensile strength of the Ganoderma lucidum mycelium fiber-based induction chip embedded paper. A smoothness tester was used to measure the surface smoothness of the Ganoderma lucidum mycelium fiber-based induction chip embedded paper. A stiffness tester was used to measure the stiffness of the Ganoderma lucidum mycelium fiber-based induction chip embedded paper. A glossiness tester was used to measure the glossiness of the Ganoderma lucidum mycelium fiber-based induction chip embedded paper. The specific values are shown in Table 1:

[0077] Table 1. Parameters of the Ganoderma lucidum mycelium fiber-based induction chip embedded paper prepared through Examples 1 - 5.

[0078]

[0079] As can be seen from Table 1, compared with Example 1, Examples 2 and 3 are Ganoderma lucidum mycelium fiber-based induction chip-embedded papers prepared from different ratios of solid culture medium raw materials, and there are some differences in their various parameters. Example 1 meets the following conditions: the contact angle between the Ganoderma lucidum mycelium fiber-based induction chip-embedded paper and water is not less than 79°; folding the Ganoderma lucidum mycelium fiber-based induction chip-embedded paper 100 times will not affect the induction function of the induction chip; the tensile strength of the Ganoderma lucidum mycelium fiber-based induction chip-embedded paper is not less than 180 MPa; the surface smoothness of the Ganoderma lucidum mycelium fiber-based induction chip-embedded paper is not less than 600 s; the stiffness of the Ganoderma lucidum mycelium fiber-based induction chip-embedded paper is not less than 0.1 mN·m; the gloss of the Ganoderma lucidum mycelium fiber-based induction chip-embedded paper is not less than 70%.

[0080] Compared with Example 1, Examples 4 and 5 are Ganoderma lucidum mycelium fiber-based induction chip-embedded papers prepared from different inoculation amounts of Ganoderma lucidum strains, and there are some differences in their various parameters. Example 4 meets the following conditions: the contact angle between the Ganoderma lucidum mycelium fiber-based induction chip-embedded paper and water is not less than 79°; folding the Ganoderma lucidum mycelium fiber-based induction chip-embedded paper 100 times will not affect the induction function of the induction chip; the tensile strength of the Ganoderma lucidum mycelium fiber-based induction chip-embedded paper is not less than 180 MPa; the surface smoothness of the Ganoderma lucidum mycelium fiber-based induction chip-embedded paper is not less than 600 s; the stiffness of the Ganoderma lucidum mycelium fiber-based induction chip-embedded paper is not less than 0.1 mN·m; the gloss of the Ganoderma lucidum mycelium fiber-based induction chip-embedded paper is not less than 70%.

[0081] The steps in the method of the embodiments of the present invention can be adjusted, combined and deleted according to actual needs. In the solution of the present invention, the descriptions of each embodiment have their own emphases. For parts not detailed or recorded in a certain embodiment, reference can be made to the relevant descriptions of other embodiments. The technical features of the technical solution of the present invention can be combined arbitrarily. For the sake of concise description, not all possible combinations of the technical features in the embodiments are described. However, as long as the combinations of these technical features do not conflict, they should be considered as the scope recorded in the present invention.

[0082] The above are only the preferred embodiments of the present application. It should be noted that for those of ordinary skill in the art, without departing from the principle of the present application, several improvements and refinements can be made, and these improvements and refinements should also be regarded as the protection scope of the present application.

Claims

1. A preparation method of an edible and medicinal fungus mycelium fiber-based induction chip embedded paper, characterized in that, It includes the following steps: Step 1, cultivation of aerial hyphae of edible and medicinal fungi: Inoculate the strain of edible and medicinal fungi into a solid medium with an inoculation amount of 5% - 10%, and place it in a culture room for cultivation to obtain aerial hyphae of edible and medicinal fungi; Step 2, drying of aerial hyphae of edible and medicinal fungi with an induction chip: Place a clean-surface induction chip in the aerial hyphae of edible and medicinal fungi, culture for 1 - 3 days to obtain aerial hyphae of edible and medicinal fungi with an induction chip, take it out for drying treatment to obtain dried aerial hyphae of edible and medicinal fungi with an induction chip; Step 3, preparation of fungal fibers of edible and medicinal fungi: Take out the induction chip covered with aerial hyphae of edible and medicinal fungi from the dried aerial hyphae of edible and medicinal fungi with an induction chip for standby, then grind the remaining dried aerial hyphae of edible and medicinal fungi with water to make a fungal fiber liquid of edible and medicinal fungi. After centrifuging multiple times, obtain fungal fibers of edible and medicinal fungi; Step 4, preparation of fungal fiber pulp of edible and medicinal fungi: Add water to the fungal fibers of edible and medicinal fungi to prepare a suspension of fungal fibers of edible and medicinal fungi, then add sodium carboxymethylcellulose to obtain suspension 1, stir with a magnetic stirrer, then add cationic starch to obtain suspension 2, and stir with a magnetic stirrer again to obtain fungal fiber pulp of edible and medicinal fungi; Step 5, preparation of edible and medicinal fungal fiber paper embedded with an induction chip: Add water to the fungal fiber pulp of edible and medicinal fungi to prepare an edible and medicinal fungal pulp suspension, filter it with a vacuum filter to obtain two edible and medicinal fungal fiber filter cakes. Spread one of the edible and medicinal fungal fiber filter cakes in a mold, put the induction chip covered with aerial hyphae of edible and medicinal fungi in step 3, and then put the other edible and medicinal fungal fiber filter cake to form an edible and medicinal fungal fiber filter cake embedded with an induction chip. Place it in a sheet former to make it form, dehydrate and dry to obtain an edible and medicinal fungal fiber-based induction chip-embedded paper.

2. The preparation method of an edible and medicinal fungal mycelium fiber-based induction chip embedded paper according to claim 1, characterized in that, The edible and medicinal fungi in step 1 are selected from any one of Ganoderma lucidum, Phellinus igniarius, Lentinula edodes, Auricularia auricula, Hericium erinaceus, Grifola frondosa, Poria cocos.

3. The preparation method of an edible and medicinal fungal mycelium fiber-based induction chip embedded paper according to claim 2, characterized in that, The preparation method of the solid medium in step 1 is: put the solid medium raw material into a cultivation bag, make small holes in the center of the bag body of the cultivation bag; sterilize at 115 - 121 °C for 20 - 120 min; wait for the solid medium raw material to cool to 25 - 30 °C to obtain the solid medium.

4. The preparation method of an edible and medicinal fungal mycelium fiber-based induction chip embedded paper according to claim 3, characterized in that, The solid medium in step 1 includes a solid medium raw material and water, and the solid medium raw material includes: 70% - 78% sawdust, 20% - 26% bran, 1% - 2% gypsum, 1% - 2% sugar.

5. The preparation method of an edible and medicinal fungal mycelium fiber-based induction chip embedded paper according to claim 4, characterized in that, After step 1, the method further includes: screening the aerial hyphae of edible and medicinal fungi with a white color, no abnormal discoloration, and uniform and dense growth by the naked eye observation method; screening the aerial hyphae of edible and medicinal fungi with thick morphology and a hyphal diameter not less than 1 μm by the scanning electron microscope observation method.

6. The preparation method of an edible and medicinal fungal mycelium fiber-based induction chip embedded paper as described in claim 5, characterized in that, The specific method of the drying treatment in step 2 is: put the aerial hyphae of edible and medicinal fungi with an induction chip into a constant temperature forced air drying oven at a temperature of 45 - 65 °C and dry for 5 - 10 h.

7. The preparation method of an edible and medicinal fungal mycelium fiber-based induction chip embedded paper according to claim 6, characterized in that, After the step 2, the method further includes: screening by reduced-pressure drying to obtain dried medicinal and edible fungus aerial hyphae with an induction chip and a water content of not more than 4%.

8. The preparation method of an edible and medicinal fungal mycelium fiber-based induction chip embedded paper according to claim 7, characterized in that, The specific method for preparing the medicinal and edible fungus mycelial fiber in the step 3 is as follows: Take out the induction chip covered with the aerial hyphae of the medicinal and edible fungus from the dried aerial hyphae of the medicinal and edible fungus with an induction chip for standby, and then grind the remaining dried aerial hyphae of the medicinal and edible fungus with water to prepare a medicinal and edible fungus mycelial fiber solution. Place the medicinal and edible fungus mycelial fiber solution in a water bath at 45-65°C and stir at a rotation speed of 200-300 r / min for 60-120 min; then, place the medicinal and edible fungus mycelial fiber solution in a centrifuge and centrifuge at 3500-4500 r / min for 10-15 min; rinse the centrifuged precipitate with pure water and then centrifuge at 3500-4500 r / min for 10-15 min to obtain the precipitate as the medicinal and edible fungus mycelial fiber.

9. The preparation method of an edible and medicinal fungal mycelium fiber-based induction chip embedded paper according to claim 8, wherein, The specific method for grinding the remaining dried aerial hyphae of the medicinal and edible fungus with water in the step 3 is as follows: put the dried aerial hyphae of the medicinal and edible fungus into a pulper, add pure water to obtain a suspension of the aerial hyphae of the medicinal and edible fungus, and make the concentration of the aerial hyphae of the medicinal and edible fungus in the suspension of the aerial hyphae of the medicinal and edible fungus 40-60 g / 100 mL. Set the gap distance between the grinding discs to 1-5 mm, the power of the flying knife rotor to 1.1 kW, and the rotation speed of the flying knife to 1430-1470 r / min, and grind the suspension of the aerial hyphae of the medicinal and edible fungus for 5-15 min to obtain a medicinal and edible fungus mycelial fiber solution.

10. The preparation method of an edible and medicinal fungal mycelium fiber-based sensing chip embedded paper according to claim 9, characterized in that, After the step 3, the method further includes: screening by a laser confocal fluorescence microscope to obtain medicinal and edible fungus mycelial fibers with a length of not less than 20 μm and a width of not more than 1 μm.

11. The preparation method of an edible and medicinal fungal mycelium fiber-based induction chip embedded paper according to claim 10, characterized in that, The specific method for preparing the medicinal and edible fungus mycelial fiber pulp in the step 4 is as follows: prepare a suspension of the medicinal and edible fungus mycelial fiber by adding water to the medicinal and edible fungus mycelial fiber, add sodium carboxymethylcellulose to obtain suspension 1, stir with a magnetic stirrer, and stir suspension 1 at 45-65°C at a rotation speed of 150-200 r / min for 15-45 min, then add cationic starch to obtain suspension 2, and then stir with a magnetic stirrer, and stir suspension 2 at 45-65°C at a rotation speed of 150-200 r / min for 15-45 min to obtain the medicinal and edible fungus mycelial fiber pulp.

12. The preparation method of an edible and medicinal fungal mycelium fiber-based induction chip embedded paper according to claim 11, characterized in that, In the step 4, the concentration of the medicinal and edible fungus mycelial fiber in the suspension of the medicinal and edible fungus mycelial fiber is 30-60 g / 100 mL, the concentration of sodium carboxymethylcellulose in suspension 1 is 0.2-1.2 g / 100 mL, and the concentration of cationic starch in suspension 2 is 0.4-1.4 g / 100 mL.

13. The preparation method of an edible and medicinal fungal mycelium fiber-based induction chip embedded paper as described in claim 12, characterized in that, After the step 4, the method further includes: screening by a potentiometer to obtain a surface charge of the medicinal and edible fungus mycelial fiber pulp of not less than 8 mV, and screening by a particle size analyzer to obtain an average particle size of the medicinal and edible fungus mycelial fiber pulp of not less than 1 mm.

14. The preparation method of an edible and medicinal fungal mycelium fiber-based induction chip embedded paper as described in claim 13, characterized in that, In step 5, the concentration of the edible and medicinal mushroom mycelial fiber in the edible and medicinal mushroom pulp suspension is 0.7-1.4 g / 100 mL; the gram weight of the edible and medicinal mushroom mycelial fiber filter cake embedded with the induction chip in step 5 is 50-100 g / m2; the specific conditions for dehydration and drying in step 5 are: heating power 10-20 kw, drying temperature 40-60 °C, and drying time 12-24 h.

15. The preparation method of an edible and medicinal fungal mycelium fiber-based induction chip embedded paper according to claim 14, characterized in that, After step 5, the method further includes: using a contact angle measuring instrument to screen that the contact angle between the edible and medicinal mushroom mycelial fiber-based induction chip embedded paper and water is not less than 79°; using a folding endurance tester to screen that the edible and medicinal mushroom mycelial fiber-based induction chip embedded paper will not affect the induction function of the induction chip after 100 folds; using an electronic universal tester to screen that the tensile strength of the edible and medicinal mushroom mycelial fiber-based induction chip embedded paper is not less than 180 MPa; using a smoothness tester to screen that the surface smoothness of the edible and medicinal mushroom mycelial fiber-based induction chip embedded paper is not less than 600 s; using a stiffness tester to screen that the stiffness of the edible and medicinal mushroom mycelial fiber-based induction chip embedded paper is not less than 0.1 mNm; using a glossiness tester to screen that the glossiness of the edible and medicinal mushroom mycelial fiber-based induction chip embedded paper is not less than 70%.

16. An edible and medicinal mushroom mycelial fiber-based induction chip embedded paper prepared by the preparation method according to any one of claims 1-15.

17. The application of the paper prepared by the preparation method according to any one of claims 1-15 as a wrapping paper.

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