Application of piper cubeba lactone and cacumen biotae extract containing piper cubeba lactone in promoting hair growth and preventing hair loss

Abstract

The invention provides application of piper cubeba lactone and cacumen biotae extract containing piper cubeba lactone in promoting hair growth and preventing hair loss, and relates to the technical field of daily chemicals. And a theoretical basis is provided for hair loss prevention / hair growth promotion of the cacumen biotae and the active ingredient piper cubeba lactone thereof. The anti-hair loss / hair growth promotion potential of the active ingredient piper cubeba lactone of the cacumen biotae extract is discussed through a 5alpha-reductase inhibition rate experiment, and a theoretical basis is provided for application of the cacumen biotae extract and the piper cubeba lactone.

Description

Technical Field

[0001] The present invention relates to the technical field of daily chemical products, and particularly relates to the application of cubebin lactone and a Platycladus orientalis extract containing cubebin lactone in promoting hair growth and preventing hair loss. Background Art

[0002] Hair loss is a common skin disease that affects the quality of life of patients and has an impact on their psychology. The causes and mechanisms of hair loss have not been fully elucidated, but it is generally believed that it may be jointly caused by genetics and environmental stimuli.

[0003] Modern medical research has proven that genetic susceptibility and abnormal local androgen metabolism in the scalp are the main factors in the pathogenesis of androgenetic alopecia. 5α - reductase controls androgen metabolism and converts the androgen testosterone (T) into dihydrotestosterone (DHT). As a potent androgen, dihydrotestosterone has a higher affinity for androgen receptors than testosterone, and high levels of dihydrotestosterone in the body lead to androgenetic alopecia. Clinically, 5α - reductase inhibitors are used to treat androgenetic alopecia by inhibiting the activity of 5α - reductase, selectively blocking the synthesis of dihydrotestosterone, and reducing the level of androgens in the body, such as steroidal drugs like finasteride and dutasteride, but they often have strong side effects.

[0004] Platycladus orientalis has been widely used in traditional Chinese medicine for the treatment of hair loss. It is considered to be able to cool blood and stop bleeding, clear heat and detoxify, effectively inhibit scalp inflammation, and promote hair follicle health. Especially for hair loss problems caused by excessive sebum secretion or hair follicle blockage, Platycladus orientalis shampoo can help improve the scalp environment, promote hair growth, and thus reduce hair loss. Platycladus orientalis also has the function of regulating scalp sebum secretion. It can help control excessive scalp oil, thereby reducing greasiness and dandruff production. This is a very useful feature for people who often feel scalp discomfort due to excessive sebum secretion or need to wash their hair frequently. As a natural ingredient, Platycladus orientalis is relatively safe to use and suitable for most people. However, the active ingredients of traditional Chinese medicine are complex and diverse, the standards are difficult to control, and the action targets are difficult to clarify.

[0005] In view of this, the present invention is specifically proposed. Summary of the Invention

[0006] One object of the present invention is to provide the application of cubebin lactone and a Platycladus orientalis extract containing cubebin lactone in the preparation of products for promoting hair growth and / or preventing hair loss, so as to solve at least one of the technical problems existing in the above - mentioned prior art.

[0007] Another object of the present invention is to provide the application of cubebin lactone in inhibiting 5α - reductase or in the preparation of products for inhibiting 5α - reductase.

[0008] A third object of the present invention is to provide the application of the Platycladus orientalis extract containing cubebin lactone in promoting the growth of dermal papilla cells.

[0009] In order to achieve the above objects of the present invention, the following technical solutions are specifically adopted: In a first aspect, the present invention provides the application of cubebin lactone and the Platycladus orientalis extract containing cubebin lactone in the preparation of products for promoting hair growth and / or preventing hair loss.

[0010] In a second aspect, the present invention provides the application of cubebin lactone in inhibiting 5α-reductase or in the preparation of products for inhibiting 5α-reductase.

[0011] In a third aspect, the present invention provides the application of the Platycladus orientalis extract containing cubebin lactone in promoting the growth of dermal papilla cells.

[0012] Further, the Platycladus orientalis extract is prepared by alcohol extraction.

[0013] Further, the preparation method of the Platycladus orientalis extract includes uniformly mixing the crude powder of Platycladus orientalis and 95% ethanol according to a volume ratio of 1:12 to 17, soaking, ultrasonic treatment, and filtering to obtain the Platycladus orientalis extract.

[0014] Further, the crude powder of Platycladus orientalis is uniformly mixed with the solvent according to a volume ratio of 1:15.

[0015] Further, the soaking time is at least 1 h.

[0016] Further, the ultrasonic treatment time is 20 to 50 min.

[0017] Further, the number of times of soaking, ultrasonic treatment, and filtering is at least 2 times, and the filtrates are combined after filtering to obtain the Platycladus orientalis extract.

[0018] Further, the particle size of the crude powder of Platycladus orientalis is 40 to 60 mesh.

[0019] For the application of cubebin lactone and the Platycladus orientalis extract containing cubebin lactone provided by the present invention in the preparation of products for promoting hair growth and / or preventing hair loss, the inventor systematically analyzed the material basis and key targets of Platycladus orientalis for promoting hair growth / preventing hair loss by network pharmacology, providing a theoretical basis for Platycladus orientalis and its active ingredient cubebin lactone to prevent hair loss / promote hair growth. The potential of the active ingredient cubebin lactone in the Platycladus orientalis extract to prevent hair loss / promote hair growth was explored through a 5α-reductase inhibition rate experiment, providing a theoretical basis for the application of the Platycladus orientalis extract and cubebin lactone. Description of the Drawings To more clearly illustrate the specific embodiments of the present invention or the technical solutions in the prior art, the following will briefly introduce the drawings required for the description of the specific embodiments or the prior art. Obviously, the drawings in the following description are some embodiments of the present invention. For those of ordinary skill in the art, without creative efforts, other drawings can also be obtained based on these drawings.

[0020] Figure 1 Venn diagram of the active ingredients of Platycladus orientalis extract - hair loss targets provided in Example 1 of the present invention; Figure 2 Core target network diagram of the active anti - hair loss / hair growth promotion of Platycladus orientalis extract provided in Example 1 of the present invention; Figure 3 Inhibitory rate of cubebin on 5α - reductase provided in Example 2 of the present invention; Figure 4 Results of the effects of Platycladus orientalis alcohol extract and Platycladus orientalis water extract on the viability of human dermal papilla cells provided in Example 3 of the present invention; Figure 5 Diagram showing the changes in hair growth on the back skin of mice promoted by Platycladus orientalis alcohol extract provided in Example 3 of the present invention. Specific embodiments

[0021] Unless otherwise defined herein, scientific and technical terms used in conjunction with the present invention shall have the meanings commonly understood by those of ordinary skill in the art. The meanings and scopes of the terms should be clear. However, in any case of potential ambiguity, the definitions provided herein shall prevail over any dictionary or extrinsic definition. In this application, unless otherwise specified, the use of "or" means "and / or". In addition, the use of the term "comprising" and other forms is non - restrictive.

[0022] The following will clearly and completely describe the technical solutions of the present invention in conjunction with the embodiments. Obviously, the described embodiments are some, but not all, of the embodiments of the present invention. Based on the embodiments of the present invention, all other embodiments obtained by those of ordinary skill in the art without creative efforts fall within the scope of protection of the present invention.

[0023] The present invention provides, on the one hand, the application of cubebin and Platycladus orientalis extract containing cubebin in the preparation of products for promoting hair growth and / or preventing hair loss.

[0024] The inventor systematically analyzed the material basis and key targets of Platycladus orientalis for promoting hair growth and preventing hair loss using network pharmacology, providing a theoretical basis for the anti-hair loss and hair growth promotion effects of Platycladus orientalis and its active ingredient, cubebin lactone. The anti-hair loss and hair growth promotion potential of the active ingredient cubebin lactone in the extract of Platycladus orientalis was explored through a 5α-reductase inhibition rate experiment, providing a theoretical basis for the application of the extract of Platycladus orientalis and cubebin lactone.

[0025] Among them, cubebin lactone is an active ingredient in the extract of Platycladus orientalis, and its CAS is 26543-89-5.

[0026] According to another aspect of the present invention, there is also provided the use of cubebin lactone in inhibiting 5α-reductase or in the preparation of a product for inhibiting 5α-reductase.

[0027] According to another aspect of the present invention, there is also provided the use of the extract of Platycladus orientalis containing cubebin lactone in promoting the growth of dermal papilla cells.

[0028] Cubebin lactone is a fat-soluble component and is insoluble in water. The content of fat-soluble components such as cubebin lactone in the water extract is limited; the extract of Platycladus orientalis obtained by alcohol extraction has a better effect in improving the viability of dermal papilla cells compared to the water extract of Platycladus orientalis. In some specific embodiments, the extract of Platycladus orientalis containing cubebin lactone is prepared by alcohol extraction. In some specific embodiments, the preparation method of the extract of Platycladus orientalis includes uniformly mixing the crude powder of Platycladus orientalis with 95% ethanol according to a volume ratio of 1:12 to 17, soaking, ultrasonic treatment, and filtering to obtain the extract of Platycladus orientalis.

[0029] In some specific embodiments, the crude powder of Platycladus orientalis is uniformly mixed with the solvent according to a volume ratio of 1:15.

[0030] In some specific embodiments, the soaking time is at least 1 h. In some specific embodiments, the ultrasonic treatment time is 20 to 50 min. In some specific embodiments, the number of times of soaking, ultrasonic treatment, and filtering is at least 2 times, and the filtrates are combined after filtering to obtain the extract of Platycladus orientalis. In some specific embodiments, the particle size of the crude powder of Platycladus orientalis is 40 to 60 mesh.

[0031] The present invention will be further described below through examples. Unless otherwise specified, the materials in the examples are prepared according to existing methods or directly purchased from the market.

[0032] Preparation of Platycladus orientalis leaf alcohol extract containing cubebin lactone: The dried crude powder of Platycladus orientalis leaves crushed to 40-60 mesh was mixed and stirred evenly with 95% ethanol at a solid-liquid ratio of 1:15, soaked at room temperature for 1 h, sonicated in an ultrasonic instrument for 30 min, filtered, and the filter residue was centrifuged, and then the extraction was repeated twice. After combining the filtrates, it was concentrated on a rotary evaporator to obtain the Platycladus orientalis leaf alcohol extract, which was stored at -20 °C.

[0033] Preparation of Platycladus orientalis leaf water extract: The difference from the preparation of Platycladus orientalis leaf alcohol extract is that deionized water is used instead of 95% ethanol to obtain the Platycladus orientalis leaf water extract.

[0034] Example 1 Network pharmacology analysis of Platycladus orientalis extract 1. Network pharmacology method of Platycladus orientalis extract (1)Screening of active ingredients of Platycladus orientalis extract and prediction of active ingredient targets Using Platycladus orientalis and its Latin name Platycladus orientalis as search terms, all its chemical components were retrieved through TCMSP. Chemical components that simultaneously meet the molecular weight < 500 molar mass and drug-likeness (DL) ≥ 0.18, as well as the active ingredients of Platycladus orientalis extract obtained by liquid chromatography-mass spectrometry analysis, were selected as potential active ingredients. Combining the literature reports, the unpredicted active ingredients were supplemented to construct a dataset of active ingredients of Platycladus orientalis.

[0035] Through the Pubchem database, the 3D structures and SMILES formats of the active ingredients of Platycladus orientalis were obtained, and then imported into the Swisstargetprediction database to obtain the corresponding predicted targets, and the relevant targets with Probability > 0 were selected. The target names were converted into unified gene names using the Uniport database, and the species was limited to Homo sapiens.

[0036] (2)Prediction of alopecia disease targets Keywords "alopecia" and its synonym "hair loss" were entered in the GeneCard, OMIM, TTD and databases to retrieve genes related to hair loss. Targets with a score greater than 10 were screened in the GeneCard database, and all disease targets in the OMIM and TTD databases were obtained. The results were integrated and duplicates were removed to obtain the targets of alopecia diseases.

[0037] (3)Construction of PPI network of Platycladus orientalis active ingredient - alopecia targets and screening of core components Intersect the target genes of the active ingredients of Platycladus orientalis leaves with the target genes related to hair loss, draw a Venn diagram, and obtain the common gene targets for Platycladus orientalis leaves to treat hair loss. Upload the intersecting targets screened by mapping to the String database, select "Homo sapiens" as the research species, and download the "tsv" format file of the protein interaction relationship. Subsequently, import the results into Cytoscape 3.10.0 software for visualization and analysis, and then use the CytoNCA plug-in tool for topological analysis. According to the "Degree" value in the software analysis results, screen out the core ingredients of the drug.

[0038] Upload the intersecting targets screened by mapping to the String database, select "Homo sapiens" as the research species, and download the "tsv" format file of the protein interaction relationship. Subsequently, import the results into Cytoscape 3.10.0 software for visualization and analysis, and then use the Centiscape plug-in tool for analysis. Screen the key core targets for the anti-hair loss / hair growth promotion of Platycladus orientalis leaf extract according to the values of "Betweenness (betweenness centrality)", "Closeness (closeness centrality)", and "Degree (degree centrality)" in the software analysis results.

[0039] 2. Network pharmacology result analysis of Platycladus orientalis leaf extract (1)Screening of active ingredients of Platycladus orientalis leaf extract and prediction of active ingredient targets A total of 63 active ingredients were screened from Platycladus orientalis leaf extract. The action targets of these 63 active ingredients were combined and duplicate ones were deleted, and a total of 630 potential action targets of the active ingredients of Platycladus orientalis leaf extract were obtained.

[0040] (2)Prediction of alopecia disease targets Combine the Genecards, OMIM, and TTD databases to summarize the targets related to alopecia. After merging, duplicate values were deleted, and finally 1,648 targets related to hair loss were obtained.

[0041] Draw a Venn diagram of the active ingredient targets of Platycladus orientalis leaf extract and the alopecia disease targets, as Figure 1 shown, and 124 common targets of Platycladus orientalis leaf extract components - hair loss were obtained. These overlapping genes are important genes for Platycladus orientalis leaf extract to promote hair growth / anti-hair loss.

[0042] (3)Construction of the PPI network of Platycladus orientalis leaf extract active ingredient - hair loss targets and screening of core ingredients With the help of Cytoscape 3.10.0 software, an interaction network of "drug-active ingredient-target-disease" was further constructed. Further analysis of the obtained result network found that it contained 124 nodes and 1726 edges, and each edge represented the relationship between Platycladus orientalis extract and active ingredients, active ingredients and targets, and targets and diseases.

[0043] Degree (degree centrality) is closely related to the targets of alopecia diseases. The larger the Degree value corresponding to the active ingredient, the closer its relationship with preventing hair loss; after sorting the characteristic parameters of the network nodes of potential active ingredients of Platycladus orientalis by Degree (Degree > 20), it was found that the Degree of cubebin was the largest (30). Therefore, cubebin may play a leading role in the process of Platycladus orientalis treating hair loss.

[0044] The target protein interaction network showed that 26 potential core targets for Platycladus orientalis extract to treat hair loss / grow hair were screened out, namely: STAT3, TP53, ERBB2, EGFR, BCL2, AKT1, CTNNB1, MAPK1, IL2, CCND1, EP300, APP, ALB, MTOR, JUN, TNF, AR, ESR1, SRC, SNCA, ACE, PPARG, CXCL8, ESR2, SHH, CYP19A1. The information of the core targets of the active ingredients of Platycladus orientalis extract for preventing hair loss / growing hair is shown in Table 1. The network diagram of the core targets of the active ingredients of Platycladus orientalis extract for preventing hair loss / growing hair is as Figure 2 shown.

[0045] Table 1

[0046] Example 2 Study on the effect of cubebin on preventing hair loss / growing hair 1. Research method for the effect of cubebin on preventing hair loss / growing hair Cubebin: CAS is 26543-89-5, purchased from TargetMol.

[0047] (1) Preparation of experimental samples: Dissolve cubebin in absolute ethanol to prepare stock solutions of cubebin with concentrations of 20 μmol / L, 40 μmol / L, 80 μmol / L, 160 μmol / L, and 320 μmol / L respectively.

[0048] (2) Test of 5α-reductase inhibition rate ① Stimulation of the test sample on the standard enzyme A. Prepare the standardized enzyme, dilute it to a concentration of 80 U / L, spread it evenly on the cell culture plate, and add 100 μL of the volume to each well. B. Prepare the samples (experimental samples in (1)). According to the preliminary experiment, configure the concentration points of the test samples to stimulate the standard enzyme. For the blank control, use sterile enzyme-free water and absolute ethanol for stimulation, with a stimulation concentration of 100 μL. The time is 6 h. Then, transfer the supernatant to an EP tube, centrifuge to obtain the supernatant for testing.

[0049] C. Select the 5α-reductase kit to detect the enzyme amount. ② Detection with the kit A. Sample addition: Accurately add 50 μL of the standard product and the sample to the enzyme-coated plate. When adding the sample, add it to the bottom of the enzyme plate well, try not to touch the well wall, and gently shake to mix evenly. B. Incubation: Seal the plate with a sealing film and incubate at 37 °C for 30 min. C. Solution preparation: Dilute the 30-fold concentrated washing solution 30-fold with distilled water and set aside for use. D. Washing: Carefully remove the sealing film, discard the liquid, shake dry, fill each well with the washing solution, let it stand for 30 seconds and then discard. Repeat this 5 times, and pat dry. E. Enzyme addition: Add 50 μL of the enzyme-labeled reagent to each well, except for the blank well.

[0050] F. Incubation: The operation is the same as B.

[0051] G. Washing: The operation is the same as D.

[0052] H. Color development: First add 50 μL of color developer A to each well, then add 50 μL of color developer B, gently shake to mix evenly, and develop color at 37 °C in the dark for 15 minutes.

[0053] I. Termination: Add 50 μL of the termination solution to each well to terminate the reaction (at this time, the blue color immediately turns yellow). J. Measurement: Zero with the blank well, and sequentially measure the absorbance (OD value) of each well at a wavelength of 450 nm. The measurement should be carried out within 15 min after adding the termination solution.

[0054] ③ The calculation formula for the enzyme activity of the test sample is as follows: 。

[0055] 2. Research results on the anti-hair loss / hair growth promotion effect of cubeb lactone The inhibition rate of cubeb lactone on 5α-reductase is shown in Table 2 and Figure 3 as shown. It can be seen that with the increase in concentration, the inhibition rate of cubeb lactone on 5α-reductase increases accordingly, indicating that the inhibitory activity of cubeb lactone on 5α-reductase shows an obvious dose-effect relationship within a certain concentration range. The IC50 of cubeb lactone on 5α-reductase is 127.938 μmol / L. Cubeb lactone can achieve the effect of anti-hair loss / hair growth promotion by inhibiting 5α-reductase.

[0056] Table 2

[0057] Evaluation of the anti - hair loss / hair growth promoting effect of the ethanol extract of Platycladus orientalis leaves in Example 3 1. Evaluation method (1)Effect of the ethanol extract of Platycladus orientalis leaves and the water extract of Platycladus orientalis leaves on the proliferation of human dermal papilla cells ① Seeding cells: Human dermal papilla cells in the logarithmic growth phase were taken, and the cell density was adjusted to 5×10 4 cells / mL. 100 μL / well was inoculated into a 96 - well plate (the edge wells were filled with sterile PBS), and incubated at 37℃ and 5% CO2 in a constant temperature. ② Grouping and drug administration: After the cells adhered, the culture medium was replaced with the culture medium containing different concentration gradients of the ethanol extract of Platycladus orientalis leaves and the water extract of Platycladus orientalis leaves for culture. The concentration gradients included 5 μg / mL, 10 μg / mL, 50 μg / mL, 100 μg / mL, and 200 μg / mL. A blank control group (Control) and a positive control group (Minoxidil) were set respectively. There were 7 replicates for each treatment, and minoxidil was used in the positive control group.

[0058] ③ CCK8 detection: After the drug acted for 48 h, the supernatant was aspirated, and 110 μL / well of the diluted CCK8 working solution was added; the culture plate was gently shaken several times and incubated at 37℃ and 5% CO2 in a constant temperature for another 2 h. The absorbance value of each well was measured at 450 nm, and the cell proliferation rate was calculated. (2)Effect of the ethanol extract of Platycladus orientalis leaves on hair growth in mice C57BL / 6 mice were used to establish an alopecia animal model. Hair growth in mice includes the telogen, catagen, and anagen phases, which is called the hair growth cycle. The depilated skin begins to darken as the hair follicles transition from the telogen to the anagen phase. C57BL / 6 mice have melanocytes in their hair follicles. When in the anagen phase, a large amount of melanin can be synthesized, and the skin is black; during the catagen phase, melanin synthesis decreases, and the skin is gray; during the telogen phase, no melanin is synthesized, and the skin is pink. C57BL / 6 mice at 6 - 8 weeks of age have hair follicles entering the telogen phase. Therefore, during this period, hair growth is slow, and the back skin is black. By observing the back skin color and hair coverage rate of mice after drug administration, it is judged whether the drug can promote the hair follicles of mice to enter the anagen phase.

[0059] ① Experimental grouping C57BL / 6 (SPF - grade) male mice were randomly divided into 4 groups, with 6 mice in each group. They were housed separately in cages and numbered, including a blank group, a model group, a positive group, and a Platycladus orientalis group. In the positive group, minoxidil was administered to the model mice, and in the Platycladus orientalis group, the ethanol extract of Platycladus orientalis leaves was administered to the model mice. The blank group and the model group used deionized water instead of drugs.

[0060] ②Model establishment After 7 days of adaptive feeding, when the mice entered the telogen phase, except for the blank group, the C57BL / 6 mice in other groups were anesthetized by intraperitoneal injection of chloral hydrate (4%, w / v). The hair on the back was shaved off completely with a razor, leaving a blank area of 2*2 cm to establish the model.

[0061] ③Drug administration After the model was established, 0.32 ml of the corresponding liquid medicine was applied to the back of the mice respectively for 20 days, once a day. After drug administration, the back of the mice was covered with a plastic wrap of appropriate size to prevent the liquid medicine from flowing out of the back, and the mice were in an anesthetized state during the application period. During the drug administration period, the hair growth on the back of each group of mice was observed and recorded every 5 days.

[0062] ④Measurement of the coverage rate of newly generated hair On the 5th, 10th, 15th, and 20th days of drug administration, the hair coverage area on the back of the mice was calculated by Image J software, and the hair coverage rate Hair coverage (%) and the promotion effect EE (hair coverage) were calculated according to the following formula: ; .

[0063] 2. Result analysis (1) Result analysis of the proliferation of human dermal papilla cells The results were as Figure 4 shown. A was the result of the effect of the ethanol extract of Platycladus orientalis on the viability of human dermal papilla cells, and B was the result of the effect of the water extract of Platycladus orientalis on the viability of human dermal papilla cells, where *P<0.05, **P<0.01, ***P<0.001 indicated compared with the blank control group; ##P<0.01, P<0.001 indicated compared with the positive drug group.

[0064] It can be seen that both the ethanol extract and the water extract of Platycladus orientalis could significantly promote the proliferation of dermal papilla cells, which are important cells for hair follicle growth. However, the proliferation effect of the ethanol extract of Platycladus orientalis was higher than that of the water extract at a concentration of 50 µg / mL. Among them, the cell proliferation ability of the ethanol extract of Platycladus orientalis reached the strongest at 100 µg / mL, while the cell proliferation ability of the water extract of Platycladus orientalis reached the strongest at 200 µg / mL, and the maximum activity value of the cells treated with the ethanol extract of Platycladus orientalis was greater than that of the cells treated with the water extract of Platycladus orientalis; compared with the water extract of Platycladus orientalis, the ethanol extract of Platycladus orientalis had a stronger proliferation ability on dermal papilla cells.

[0065] (2) Result analysis of mouse hair growth On the 5th, 10th, 15th, and 20th days after administration to each group of mice, the changes in hair growth on the back skin were as follows Figure 5 shown. It can be seen that after the 5th day of administration, there was no significant hair growth on the backs of the mice in each group, but the skin color changed; after 10 days, the hair growth became obvious, and the hair of the mice in different groups grew to varying degrees. Among them, the promoting effect of the Platycladus orientalis group was the most obvious, indicating that the Platycladus orientalis alcohol extract could significantly shorten the transformation time of hair follicles from the resting phase to the growth phase and the time from entering the growth phase to the hair growing out of the skin surface. After 20 days, compared with the model group, the hair on the backs of the mice in each group had grown, and the hair growth in the Platycladus orientalis group was comparable to that in the positive group, minoxidil group. Therefore, the Platycladus orientalis alcohol extract has the effect of promoting hair growth.

[0066] The back hair coverage rates of each group of mice on the 5th, 10th, 15th, and 20th days are shown in Table 3.

[0067] Table 3

[0068] As can be seen from Table 1, on the 5th day after administration, no hair grew in the shaved areas of the positive group and the Platycladus orientalis group. On the 10th day after administration, the skin color on the backs of the mice in the Platycladus orientalis group changed. On the 15th day, the backs of the mice in the positive drug group began to turn black. On the 20th day, most of the backs of the mice in each group were covered with hair. Among them, from the perspective of the area covered by the newly grown hair, the Platycladus orientalis group had a better effect on promoting hair growth. The hair coverage percentage was calculated by the ratio of the area covered by the newly grown hair of each group of mice to the area of its entire shaved area, and the promotion effect of each drug on hair restoration was evaluated by the ratio (EE) of the hair coverage percentage of each drug-administered group of mice on the 12th day to the hair coverage percentage of the model group. Platycladus orientalis group > positive group (minoxidil group).

[0069] Finally, it should be noted that the above embodiments are only used to illustrate the technical solutions of the present invention, and are not intended to limit them; although the present invention has been described in detail with reference to the foregoing embodiments, those of ordinary skill in the art should understand that they can still modify the technical solutions recorded in the foregoing embodiments, or perform equivalent replacements on some or all of the technical features; and these modifications or replacements do not make the essence of the corresponding technical solutions deviate from the scope of the technical solutions of the embodiments of the present invention.

Claims

1. Application of cubebin lactone and Platycladus orientalis extract containing cubebin lactone in preparing products for promoting hair growth and / or preventing hair loss.

2. Application of cubebin lactone in inhibiting 5α - reductase or preparing products for inhibiting 5α - reductase.

3. Application of Platycladus orientalis extract containing cubebin lactone in promoting the growth of dermal papilla cells.

4. The application according to any one of claims 1 to 3, characterized in that, The Platycladus orientalis extract is prepared by alcohol extraction.

5. The application according to claim 4, characterized in that The preparation method of the Platycladus orientalis extract includes mixing the crude powder of Platycladus orientalis and 95% ethanol in a volume ratio of 1:12 - 17, soaking, ultrasonic treatment, and filtering to obtain the Platycladus orientalis extract.

6. The application according to claim 5, characterized in that, The crude powder of Platycladus orientalis and 95% ethanol are mixed evenly in a volume ratio of 1:

15.

7. The application according to claim 5, wherein The soaking time is at least 1 h.

8. The application according to claim 5, characterized in that, The ultrasonic treatment time is 20 - 50 min.

9. The application according to claim 5, wherein The number of times of soaking, ultrasonic treatment, and filtering is at least 2 times, and the filtrates are combined after filtering to obtain the Platycladus orientalis extract.

10. The application according to claim 5, characterized in that The particle size of the crude powder of Platycladus orientalis is 40 - 60 mesh.

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