Moisturizing and wrinkle-removing eye cream and preparation method thereof

Abstract

The invention discloses moisturizing and wrinkle-removing eye cream and a preparation method thereof. The moisturizing and wrinkle-removing eye cream comprises a banana fruit extract, a reed extract, a licorice root extract, an angelica root extract, a salvia miltiorrhiza root extract, a dogwood fruit extract and a boxthorn root extract. The prepared moisturizing and wrinkle-removing eye cream can deeply, durably and efficiently moisturize, can quickly eliminate wrinkles, smoothen skin and increase skin permeability and elasticity, has relatively strong anti-oxidation and anti-ultraviolet capabilities, is antibacterial and good in stability, and can absorb moisture from the environment, dynamically maintain skin water balance and play a long-acting deep moisturizing role.

Description

Technical Field

[0001] The present invention relates to the technical field of cosmetics, and particularly relates to an anti-wrinkle and moisturizing eye cream and a preparation method thereof. Background Art

[0002] The aging of people is manifested on the face, mainly concentrated around the eyes, and the most prominent symptom is the gradual increase in wrinkles. The aging of people's skin is mainly due to two reasons: one is the decrease in the water hydration ability of the stratum corneum and the reduction in the content of water-lipid emulsion on the skin surface; the other is the reduction in the synthesis of dermal collagen fibers and the degeneration of elastic fibers. At present, the commonly used anti-wrinkle ingredient in the cosmetics field is to form a thin film on the skin surface to produce a rapid tightening effect. Serum globulin derived from animals is often used, but its effect is short-lived and has strong dependence. When the external humidity is relatively low, the moisturizers used in traditional cosmetics cannot play a good moisturizing role.

[0003] With the increasing thinning of the ozone layer, the ultraviolet rays reaching the ground are constantly increasing, and ultraviolet radiation has many adverse effects on human health. Ultraviolet rays can cause skin aging, which is generally characterized by thinning, dryness, wrinkles and seborrheic keratosis of the skin. Sunscreen agents are widely used in cosmetics because they can effectively protect the human body from excessive ultraviolet radiation. However, excessive addition of sunscreen agents in cosmetics will cause certain harm to the human body, such as making the skin rough, irritating the eyes and skin, and causing photosensitivity reactions. Scientists at the University of California in the United States published a new report in the journal "Free Radical Biology and Medicine" pointing out that some chemicals in sunscreen cosmetics themselves can cause skin cancer, so sunscreen agents are the core of sunscreen cosmetic formulations.

[0004] Chemical sunscreen agents are also known as ultraviolet absorbers. Currently, the ultraviolet absorbers on the market have certain irritation. If used for a long time, they will cause skin dependence and damage to skin cells and skin structure. Therefore, new, safe and highly efficient ultraviolet absorbers have always been the research focus of sunscreen cosmetics.

[0005] Microcapsules are a protection technology that encapsulates solids, liquids or gases into small particles. Using microcapsule technology, liquid powderization can be achieved, the active substances can be isolated from the external environment, the special odors and bad colors of some components can be reduced or covered, and at the same time, the instant or slow release of components can be realized, solving many product development problems that cannot be solved due to technical obstacles. Due to the small particle size of nano-microcapsules, they have good targeting, slow release and low toxicity and side effects, so they are widely used. With the continuous development of microcapsule technology, the prepared microcapsule particle size can reach 1 - 1000nm.

[0006] CN108904414A discloses a fast wrinkle-removing eye cream, and the eye cream contains the following raw materials: dipropylene glycol, 2,6-epoxyhexene glycerol ether, hyaluronic acid, fresh-keeping ketone, acryloyldimethyltaurine ammonium / VP copolymer, hydroxypropyl methylcellulose, coenzyme Q10, cyclopentasiloxane, BT-9268, isocetane, dimethicone / vinyl dimethicone crosspolymer, sodium acrylate / sodium acryloyldimethyltaurate copolymer, ortimin, acetyl hexapeptide-8, SYN-COLL, BSASM, 1,2-hexanediol, SYN-AKE and essence. However, the fast wrinkle-removing eye cream described in this invention cannot achieve deep skin moisturizing function, and cannot repair the skin cell damage caused by ultraviolet rays.

[0007] CN110368330A discloses a moisturizing and hydrating facial cream, and the moisturizing and hydrating facial cream includes lignin nanoparticles, and the facial cream can effectively increase the moisturizing function. However, this moisturizing and hydrating facial cream does not have functions such as resisting ultraviolet rays and antibacterial, and its functions are relatively single. Summary of the Invention

[0008] Since common moisturizing and wrinkle-removing eye creams cannot deeply moisturize and remove wrinkles from the skin, and at the same time, if an antioxidant such as p-hydroxyacetophenone is added to cosmetics or skin care products, the eye cream of the present invention will change color and turn yellow due to the unstable chemical properties of itself. Making the moisturizing and wrinkle-removing components into the size of nanoparticles can maximize their efficacy. The current beauty skin care products only claim to have good moisturizing effects with a little moisturizing function. However, due to the skin barrier function, such moisturizing components are actually difficult to penetrate through the stratum corneum to reach the deep skin, and do not have the excellent moisturizing effects as advertised. Adding modified banana fruit extract nano-capsule particles to the moisturizing and wrinkle-removing eye cream composition of the present invention can not only deeply and durably moisturize, but also resist ultraviolet rays, restore damaged skin, stabilize the chemical properties of cosmetics such as color change, and antibacterial, which can be described as "one cream with multiple effects".

[0009] The present invention provides a preparation method of a moisturizing and wrinkle-removing eye cream, and the preparation steps are as follows:

[0010] Step 1: Mix 30-50 parts of water, 4-6 parts of glycerol, 2-5 parts of 1,2-butanediol, 3-5 parts of 1,3-propanediol, 3-5 parts of acrylate / C10-30 alkanol acrylate cross-linked polymer, 0.5-1 part of sodium hyaluronate, 2-7 parts of allantoin, 2-4 parts of trehalose, and 0.3-0.5 part of phosphatidylcholine by heating and stirring until evenly mixed to obtain Component A;

[0011] Step 2: Heat and stir 3 - 7 parts of tocopheryl acetate, 0.1 - 0.5 part of sunflower seed oil, 0.3 - 0.5 part of phospholipids, 3 - 5 parts of triheptanoin, and 2 - 5 parts of C14 - 22 alcohol until they are evenly mixed to obtain Component B;

[0012] Step 3: Add Component B obtained in Step 2 to Component A obtained in Step 1 and perform constant - temperature homogenization to obtain a homogenized mixture;

[0013] Step 4: Cool the homogenized mixture obtained in Step 3 to 55 - 60 °C, and add 3 - 8 parts of plantain fruit extract, 0.3 - 0.5 part of reed extract, 0.1 - 1 part of licorice root extract, 0.1 - 0.5 part of angelica root extract, 0.3 - 1 part of salvia root extract, 0.5 - 2 parts of cornel fruit extract, 0.5 - 2 parts of wolfberry root extract, 3 - 5 parts of glucosyl hesperidin, 0.5 - 1 part of tocopherol, 3 - 5 parts of arginine, 0.3 - 0.5 part of ectoine, 0.1 - 0.5 part of ergothioneine, 0.5 - 0.8 part of ascorbyl tetraisopalmitate, 3 - 5 parts of p - hydroxyacetophenone, 2 - 5 parts of 1,2 - butanediol, 3 - 5 parts of polydimethylsiloxane, 3 - 5 parts of hydrogenated rapeseed oil alcohol, 2 - 5 parts of C12 - 20 alkyl glucoside, 0.5 - 1 part of dipeptide diamino butyryl benzyl amide diacetate, and 1 - 2 parts of sodium chloride, and perform constant - temperature homogenization for 3 - 5 min to obtain a viscous composition;

[0014] Step 5: Cool the viscous composition obtained in Step 4 to room temperature, sieve it, and discharge it to obtain the moisturizing and anti - wrinkle eye cream.

[0015] The specific meaning of heating and stirring until evenly mixed in Step 1 is to stir and mix for 10 - 20 min under the conditions of 80 - 85 °C and 50 - 200 rpm.

[0016] The specific meaning of heating and stirring until evenly mixed in Step 2 is to stir and mix for 10 - 20 min under the conditions of 75 - 80 °C and 50 - 200 rpm.

[0017] The constant - temperature homogenization in Step 3 means homogenizing for 5 - 10 min under the conditions of 80 - 85 °C and 8000 - 10000 rpm.

[0018] The constant - temperature homogenization in Step 4 means homogenizing for 3 - 5 min under the conditions of 55 - 60 °C and 8000 - 10000 rpm.

[0019] Cooling to room temperature in Step 5 means stirring and cooling to room temperature under the conditions of 50 - 200 rpm.

[0020] Step 4 may also be: cooling the homogeneous mixture obtained in Step 3 to 55-60°C, adding 3-8 parts of modified plantain fruit extract, 0.3-0.5 parts of reed extract, 0.1-1 part of licorice root extract, 0.1-0.5 parts of angelica root extract, 0.3-1 part of salvia root extract, 0.5-2 parts of cornel fruit extract, 0.5-2 parts of wolfberry root extract, 3-5 parts of glucosyl hesperidin, 0.5-1 part of tocopherol, 3-5 parts of arginine, 0.3-0.5 parts of ectoine, 0.1-0.5 parts of ergothioneine, 0.5-0.8 parts of ascorbyl tetraisopalmitate, 3-5 parts of p-hydroxyacetophenone, 2-5 parts of 1,2-butanediol, 3-5 parts of polydimethylsiloxane, 3-5 parts of hydrogenated rapeseed oil alcohol, 2-5 parts of C12-20 alkyl glucoside, 0.5-1 part of dipeptide diamino butyryl benzylamide diacetate, 1-2 parts of sodium chloride, and performing isothermal homogenization for 3-5 min to obtain a viscous composition;

[0021] The preparation steps of the modified plantain fruit extract are as follows: dissolving 0.6-1.0 g of polyvinylpyrrolidone, 0.3-0.5 g of 4-dimethylaminopyridine, 0.5-0.8 mL of triethylamine, and 30-50 mg of plantain fruit extract in 25-40 mL of a sodium sulfate aqueous solution with a mass fraction of 2-3%, stirring and reacting for 22-24 h under the conditions of light avoidance and nitrogen protection, with a stirring speed of 500-800 rpm. After the reaction, transfer all the mixed liquid into a dialysis bag, immerse the dialysis bag in double-distilled water, dialyze for 2-3 d, centrifuge to obtain the precipitate, and vacuum freeze-dry it to obtain the modified plantain fruit extract;

[0022] More preferably, the preparation steps of the modified plantain fruit extract are as follows: dissolving 0.6-1.0 g of polyvinylpyrrolidone, 0.3-0.5 g of 4-dimethylaminopyridine, 0.5-0.8 mL of triethylamine, and 30-50 mg of plantain fruit extract in 25-40 mL of a sodium sulfate aqueous solution with a mass fraction of 2-3%, stirring and reacting for 22-24 h under the conditions of light avoidance and nitrogen protection, with a stirring speed of 500-800 rpm. After the reaction, transfer all the mixed liquid into a dialysis bag, immerse the dialysis bag in double-distilled water, dialyze for 2-3 d, then add 13-16 mg of a modifier thereto, heat to 80-90°C, and stir and react for 20-30 min; centrifuge to obtain the precipitate and vacuum freeze-dry it to prepare the modified plantain fruit extract.

[0023] The modifier is any one of 1-hydroxy-2-naphthoic acid, ethyl p-aminobenzoate, methyl salicylate, and methyl cinnamate; more preferably, the modifier is 1-hydroxy-2-naphthoic acid.

[0024] The functions of the main raw materials in the present invention are introduced as follows:

[0025] Glycerin, 1,2 - butanediol, 1,3 - propanediol: 1. Moisturizing: Glycerin has strong hygroscopicity and can absorb moisture in the air to provide long - lasting moisture for the skin; 2. Relieving skin dryness: Glycerin can relieve skin itching caused by skin dryness and make the skin more comfortable.

[0026] Trioctanoin: Trioctanoin is mainly used as a skin conditioner and viscosity controller in cosmetics. It is a non - ionic surfactant - ester type with excellent dispersion, emulsification, and lubrication capabilities. As a conditioner, emollient, viscosity regulator, etc., it is applied in the field of personal care products.

[0027] Trehalose: A natural moisturizing ingredient that can enhance the skin's moisturizing ability.

[0028] Polydimethylsiloxane: An emollient.

[0029] C14 - 22 alcohols: As an emulsification stabilizer. C14 - 22 alcohols are mainly used as emulsification stabilizers and skin conditioners in cosmetics. It can increase the smoothness of cosmetics, improve the texture of products, and at the same time form a protective film on the skin surface to play a moisturizing role. In addition, C14 alcohol also has antibacterial and anti - corrosion effects and can extend the shelf life of cosmetics.

[0030] 1,2 - butanediol: 1. Moisturizing: 1,2 - butanediol is a common moisturizing ingredient that can help improve the moisturizing effect of cosmetics and make the skin softer and smoother; 2. Antiseptic: 1,2 - butanediol also has antiseptic properties and can extend the shelf life of cosmetics to avoid product deterioration and contamination; 3. Improving skin feel: 1,2 - butanediol can improve the skin feel of cosmetics, make the product more delicate, easy to spread, and improve the use experience.

[0031] Glucosyl hesperidin: The main functions of glucosyl hesperidin in cosmetics are as a skin conditioner and moisturizer. It can promote blood circulation, make the skin feel warm, overcome skin cold, stress and fatigue, and thus restore the luster of tired skin. At the same time, it can also promote skin metabolism, effectively improve dark circles, improve skin nutrition, and reduce skin inflammation.

[0032] Tocopheryl acetate: The main functions of tocopheryl acetate in cosmetics are antioxidant and moisturizing. It can protect the skin from ultraviolet damage, and at the same time promote wound healing, maintain skin moisture, prevent inflammation, improve dark spots, etc. In addition, tocopheryl acetate also has the effects of nourishing the skin, anti - aging, reducing wrinkles, etc., and can be used for skin care and treating acne marks.

[0033] Hydrogenated rapeseed oil alcohol: Hydrogenated rapeseed oil alcohol is mainly used as an emollient in cosmetics. It can provide moisture and hydration to the skin, making the skin softer and smoother. At the same time, hydrogenated rapeseed oil alcohol also has the effects of improving skin feel, enhancing product stability and having an anti-corrosion function.

[0034] Acrylate / C10-30 alkyl acrylate cross-polymer: 1. Film-forming agent: This cross-polymer can form a thin film on the skin surface, playing a role in protecting the skin and preventing moisture loss. At the same time, this thin film can also increase the persistence of cosmetics, making the product stay on the skin for a longer time; 2. Thickening agent: The cross-polymer can increase the viscosity of cosmetics, making it thicker. This helps to improve the stability of the product and the feeling during application, making the product easier to use.

[0035] Arginine: 1. Moisturizing effect: Arginine can work synergistically with moisturizing ingredients such as glycolic acid and trehalose to maintain skin moisture, smooth the skin, and reduce the peeling of dandruff; 2. pH regulation effect: Arginine is alkaline and can be used instead of ammonia water in hair dyes to avoid the irritating smell of ammonia water and at the same time improve the coloring power of dyes; 3. Antioxidant: Arginine can inhibit the formation of melanin in the skin, accelerate skin metabolism, so it has a certain antioxidant effect, which helps to reduce skin aging, and can also promote protein synthesis and maintain skin elasticity; 4. Anti-inflammatory: Arginine itself can stimulate the skin tissue to produce more proteins, which is beneficial to the synthesis and repair of skin tissue, and can also inhibit the inflammatory response, so it has a certain anti-inflammatory effect; 5. Other effects: Arginine can promote the body's metabolism by improving blood circulation and accelerating the blood flow rate. In addition, arginine can help the body excrete toxins, which is beneficial to physical health.

[0036] C12-20 alkyl glucoside: The main functions of C12-20 alkyl glucoside in cosmetics are as a detergent, thickening agent, emulsifier and surfactant. It is a relatively mild detergent with a certain cleaning effect. At the same time, alkyl glucoside also has functions such as moisturizing, nourishing the skin and relieving skin dryness.

[0037] Allantoin: It has a nutritional conditioning effect on the skin and subcutaneous tissues around the eyes, used to maintain the health of the tissues around the eyes and prevent the degeneration of dermal elastic fibers.

[0038] p-Hydroxyacetophenone: 1. Antiseptic: The antiseptic effect of p-hydroxyacetophenone is mainly to inhibit the growth of microorganisms and prevent products from being infected by bacteria and fungi. In cosmetics, p-hydroxyacetophenone can maintain the cleanliness and freshness of products and prevent product deterioration; 2. Antioxidant: p-Hydroxyacetophenone has antioxidant properties, can resist the attack of free radicals, thus preventing skin aging and maintaining skin health; 3. Soothing: p-Hydroxyacetophenone can soothe the skin, reduce skin inflammation and irritation reactions, and has a certain relieving effect on sensitive and inflamed skin; 4. Synergistic effect: When p-hydroxyacetophenone is used in combination with other preservatives, it can produce a synergistic effect and improve the antiseptic effect; 5. Multifunctional: p-Hydroxyacetophenone not only has antiseptic properties, but can also be used as an antioxidant, a soother, etc., and is a multifunctional cosmetic raw material.

[0039] Banana fruit extract, Phragmites communis extract, Angelica polymorpha sinensis root extract, Salvia miltiorrhiza root extract, Cornus officinalis fruit extract, Lycium chinense root extract: 1. Moisturizing and nourishing: Banana fruit extract is rich in vitamins and natural moisturizing factors, which can help the skin absorb and lock in moisture, thus increasing skin moisture and softness; 2. Antioxidant and anti-aging: Banana fruit extract is rich in vitamins C, E and polyphenolic compounds. These antioxidant substances help neutralize free radicals, reduce oxidative damage, and thus delay the skin aging process; 3. Promote collagen synthesis: Certain components in banana fruit extract can stimulate the production of collagen, increase skin elasticity and firmness, and reduce the appearance of fine lines and wrinkles.

[0040] Ectoin: 1. Moisturizing: Ectoin can stabilize protein and cell structures, reduce the damage caused by ultraviolet rays and dryness. At the same time, it can provide good moisturizing and sunscreen functions and has the effect of whitening the skin; 2. Anti-aging: Ectoin can activate cells, repair and resist aging, tighten the skin, and effectively prevent cell aging; 3. Anti-inflammatory: Ectoin also has an anti-inflammatory effect and can protect neutrophils; 4. Soothe the skin: Ectoin can repair and regenerate aging or damaged skin, has an overall cell protection function, and can also provide long-term moisturization, improve skin roughness, repair the skin barrier, prevent aging, protect DNA, etc.

[0041] Ergothioneine: As a natural antioxidant, it also has a certain anti-inflammatory effect.

[0042] Dipeptide Diaminobutyroyl Benzylamide Diacetate: It is a chemical component commonly used in skin care products as a moisturizer and anti-aging ingredient, with the effects of promoting collagen synthesis, enhancing skin elasticity, and improving skin moisturizing performance, etc.

[0043] Ascorbyl Tetraisopalmitate: 1. Whitening: It inhibits the activity of tyrosinase in cells and melanin production, thus achieving a whitening effect; 2. Moisturizing: It has good solubility in oil and can be efficiently absorbed by fibroblasts, so it can play a moisturizing role; 3. Strong transdermal absorption: It can penetrate through the skin surface and promote the absorption of nutrients by the skin.

[0044] Phospholipids: 1. Moisturizing: Phospholipids are the main components of cell membranes. 40% - 50% of the sebum in the stratum corneum is composed of ceramides. Phospholipids can simulate and enhance the skin's ability to retain moisture and protect the skin from external factors; 2. Antioxidation: They have extremely strong antioxidant ability, can scavenge nitrogen dioxide, sulfides, disulfides, etc., and can also reduce lipid peroxidation and effectively inhibit lipid peroxidation caused by free radicals; 3. Repair damaged skin: Ceramide is the main part of the intercellular matrix and plays an important role in maintaining the water balance of the stratum corneum. It can also enhance skin elasticity, delay aging, and has a good effect on repairing damaged skin and protecting the skin barrier.

[0045] Phosphatidylcholine: 1. Moisturizing: Phosphatidylcholine has a moisturizing effect, can improve the skin's permeability and moisture retention, and improve the skin condition; 2. Antioxidation: Phosphatidylcholine has an antioxidant effect, can reduce the damage of free radicals to the skin, and delay skin aging; 3. Condition the skin: Phosphatidylcholine can condition the skin, promote the regeneration and repair of skin cells, and improve skin problems such as roughness and dryness.

[0046] Tocopherol (Vitamin E): 1. Antioxidation: Vitamin E is an antioxidant that can protect the skin from free radical damage, reduce skin aging and the formation of wrinkles; 2. Moisturizing: Vitamin E can promote the regeneration and repair of skin cells, improve the skin's moisture retention ability, and improve skin problems such as dryness and roughness; 3. Whitening: Vitamin E can inhibit melanin production, fade existing melanin, and improve skin problems such as uneven skin tone and age spots; 4. Anti-acne: Vitamin E can inhibit the reproduction of Propionibacterium acnes, reduce skin inflammation and infection, and help eliminate acne; 5. Anti-aging: Vitamin E can promote the synthesis of skin collagen, increase skin elasticity and firmness, and delay skin aging.

[0047] Sodium Hyaluronate: 1. Moisturizing: Sodium hyaluronate is a natural moisturizer that can increase the elasticity of the skin and has the effect of hydrating and moisturizing, which can improve skin dryness; 2. Nutrition: Sodium hyaluronate is an inherent biological substance in the skin. Smaller sodium hyaluronate can penetrate into the epidermal layer of the skin, promoting the supply of skin nutrition and the excretion of waste, thereby preventing skin aging and playing a role in beauty and skin care; 3. Skin damage repair and prevention: Sodium hyaluronate can promote the regeneration of damaged skin by promoting the proliferation and differentiation of epidermal cells and scavenging oxygen free radicals. Pre - use also has a certain preventive effect; 4. Anti - wrinkle: Small - molecule sodium hyaluronate can penetrate into the dermis layer, promoting blood microcirculation, which is beneficial for the skin to absorb nutrients and plays an anti - wrinkle role; 5. Anti - allergy: If the skin is stimulated by allergens, it may induce an allergic reaction, resulting in symptoms such as skin redness and itching. At this time, sodium hyaluronate can be used for treatment according to doctor's advice to achieve the anti - allergy effect; 6. Reducing pigmentation: Sodium hyaluronate has the effect of inhibiting melanocytes, which can reduce the deposition of skin melanin, thereby achieving the purpose of whitening the skin.

[0048] Sunflower (HELIANTHUS ANNUUS) Seed Oil: First of all, it is an important base oil raw material with a skin - moisturizing effect. At the same time, it also has an anti - inflammatory effect of activating peroxisome proliferator - activated receptors and promoting skin wound healing, as well as promoting the decomposition of fat cells, playing a role in weight loss; Secondly, sunflower seed oil has an inhibitory effect on tyrosinase, which can play a role in whitening; In addition, sunflower seed oil is suitable for 7 types of skin, such as dry, tolerant, fine - lined, firming, pigmented, non - pigmented, and sensitive skin.

[0049] Sodium Chloride: The main role of sodium chloride in cosmetics is thickening and stabilizing; Sodium chloride can be dissolved in water and glycerol for use and is not easily dissolved in ethanol. It has stable chemical properties. It has a thickening effect in personal care products, can also stabilize emulsions by adjusting the surface tension of oil and water, and has a certain bactericidal and anti - inflammatory effect; In addition, sodium chloride can also reduce the cost of cosmetics.

[0050] Advantages of the present invention:

[0051] 1. The present invention provides appropriate moisture and oil for the skin to protect the hydration state of the skin and the integrity of sebum.

[0052] 2. Repair damaged and aging skin, and promote skin tissue metabolism and cell formation. While effectively maintaining the moisture of the eye skin, this eye cream provides sufficient collagen nutrients, enhancing the firmness of the skin, smoothing fine lines, reducing deep wrinkles, and making the skin smooth and elastic.

[0053] 3. The moisturizing and anti-wrinkle eye cream of the present invention can deeply and durably moisturize, and at the same time can effectively resist ultraviolet rays. The present invention also makes the moisturizing and hydrating eye cream itself have relatively stable chemical properties and can also play an antibacterial role. Detailed implementation mode

[0054] In order to further understand the present invention, the application of a moisturizing and anti-wrinkle eye cream provided by the present invention will be described in detail below in conjunction with embodiments.

[0055] The sources and parameters of some raw materials in the embodiments are as follows:

[0056] The extracts of banana fruit, reed, licorice root, angelica root, salvia root, cornel fruit, and wolfberry root are all of commercial cosmetic grade.

[0057] 1-Hydroxy-2-naphthoic acid, CAS No. 86-48-6.

[0058] Polyvinylpyrrolidone, with a molecular mass of 1,300,000, is sourced from Shanghai Aladdin Biochemical Technology Co., Ltd., China.

[0059] 4-Dimethylaminopyridine, CAS No. 1122-58-3, is sourced from Sigma-Aldrich, USA.

[0060] Example 1

[0061] A preparation method of a moisturizing and anti-wrinkle eye cream is as follows:

[0062] Step 1: Stir 400 g of water, 40 g of glycerol, 20 g of 1,2-butanediol, 30 g of 1,3-propanediol, 40 g of acrylate / C10-30 alkyl acrylate cross-linked polymer, 10 g of sodium hyaluronate, 50 g of allantoin, 30 g of trehalose, and 3 g of phosphatidylcholine at 80 °C and 100 rpm for 15 min to obtain Component A;

[0063] Step 2: Stir 40 g of tocopheryl acetate, 2 g of sunflower seed oil, 5 g of phospholipid, 40 g of triheptanoin, and 30 g of C14-22 alcohol at 80 °C and 100 rpm for 15 min to obtain Component B;

[0064] Step 3: Add the obtained Component B in Step 2 to the obtained Component A in Step 1, and carry out constant-temperature homogenization at 80 °C and 8000 rpm for 8 min to obtain a homogenized mixture;

[0065] Step 4: Cool the homogeneous mixture obtained in Step 3 to 55°C with stirring at 100 rpm, and add 50 g of plantain fruit extract, 5 g of reed extract, 5 g of licorice root extract, 4 g of angelica root extract, 6 g of salvia root extract, 10 g of cornel fruit extract, 10 g of wolfberry root extract, 40 g of glucosyl hesperidin, 10 g of tocopherol, 30 g of arginine, 5 g of ectoine, 5 g of ergothioneine, 5 g of tetrahexyldecyl ascorbate, 30 g of p-hydroxyacetophenone, 30 g of 1,2-butanediol, 30 g of polydimethylsiloxane, 40 g of hydrogenated rapeseed oil alcohol, 30 g of C12-20 alkyl glucoside, 5 g of dipeptide diamino butyroyl benzylamide diacetate, and 10 g of sodium chloride. Homogenize at 55°C and 8000 rpm for 5 min, and keep warm for 8 min to obtain a viscous composition;

[0066] Step 5: Stir and cool the viscous composition obtained in Step 4 above to room temperature at 100 rpm, pass through a 200-mesh sieve, and discharge to obtain the moisturizing and anti-wrinkle eye cream.

[0067] Example 2

[0068] A method for preparing a moisturizing and anti-wrinkle eye cream comprises the following steps:

[0069] Step 1: Stir 400 g of water, 40 g of glycerol, 20 g of 1,2-butanediol, 30 g of 1,3-propanediol, 40 g of acrylate / C10-30 alkyl acrylate cross-linked polymer, 10 g of sodium hyaluronate, 50 g of allantoin, 30 g of trehalose, and 3 g of phosphatidylcholine at 80°C and 100 rpm for 15 min to obtain Component A;

[0070] Step 2: Stir 40 g of tocopheryl acetate, 2 g of sunflower seed oil, 5 g of phospholipid, 40 g of triheptanoin, and 30 g of C14-22 alcohol at 80°C and 100 rpm for 15 min to obtain Component B;

[0071] Step 3: Add the Component B obtained in Step 2 to the Component A obtained in Step 1, and keep the temperature constant and homogenize at 80°C and 8000 rpm for 8 min to obtain a homogeneous mixture;

[0072] Step 4: Cool the homogeneous mixture obtained in Step 3 to 55°C with stirring at 100 rpm, add 50 g of modified plantain fruit extract, 5 g of reed extract, 5 g of licorice root extract, 4 g of angelica root extract, 6 g of salvia root extract, 10 g of cornel fruit extract, 10 g of wolfberry root extract, 40 g of glucosyl hesperidin, 10 g of tocopherol, 30 g of arginine, 5 g of ectoine, 5 g of ergothioneine, 5 g of ascorbyl tetraisopalmitate, 30 g of p-hydroxyacetophenone, 30 g of 1,2-butanediol, 30 g of polydimethylsiloxane, 40 g of hydrogenated rapeseed oil alcohol, 30 g of C12-20 alkyl glucoside, 5 g of dipeptide diamino butyryl benzyl amide diacetate, and 10 g of sodium chloride, homogenize at 55°C and 8000 rpm for 5 min, and keep warm for 8 min to obtain a viscous composition;

[0073] Step 5: Stir and cool the viscous composition obtained in Step 4 above to room temperature at 100 rpm, pass through a 200-mesh sieve, and discharge to obtain the moisturizing and anti-wrinkle eye cream.

[0074] The preparation steps of the modified plantain fruit extract are as follows:

[0075] Dissolve 0.8 g of polyvinylpyrrolidone, 0.5 g of 4-dimethylaminopyridine, 0.8 mL of triethylamine, and 35 mg of plantain fruit extract in 40 mL of a 2% sodium sulfate aqueous solution, stir and react for 24 h under the conditions of light avoidance and nitrogen protection, with a stirring speed of 800 rpm. After the reaction, transfer all the mixed liquid into a dialysis bag, immerse the dialysis bag in double-distilled water, and dialyze for 3 d; then centrifuge to obtain the precipitate and vacuum freeze-dry to prepare the modified plantain fruit extract.

[0076] Example 3

[0077] A preparation method of a moisturizing and anti-wrinkle eye cream, the steps are as follows:

[0078] Step 1: Stir 400 g of water, 40 g of glycerol, 20 g of 1,2-butanediol, 30 g of 1,3-propanediol, 40 g of acrylate / C10-30 alkanol acrylate cross-linked polymer, 10 g of sodium hyaluronate, 50 g of allantoin, 30 g of trehalose, and 3 g of phosphatidylcholine at 80°C and 100 rpm for 15 min to obtain Component A;

[0079] Step 2: Stir 40 g of tocopheryl acetate, 2 g of sunflower seed oil, 5 g of phospholipid, 40 g of triheptanoin, and 30 g of C14-22 alcohol at 80°C and 100 rpm for 15 min to obtain Component B;

[0080] Step 3: Add the B component obtained in Step 2 to the A component obtained in Step 1, and perform constant-temperature homogenization at 80°C and 8000 rpm for 8 min to obtain a homogenized mixture;

[0081] Step 4: Cool the homogenized mixture obtained in Step 3 to 55°C by stirring at 100 rpm, add 50 g of modified plantain fruit extract, 5 g of reed extract, 5 g of licorice root extract, 4 g of angelica root extract, 6 g of salvia root extract, 10 g of cornel fruit extract, 10 g of wolfberry root extract, 40 g of glucosyl hesperidin, 10 g of tocopherol, 30 g of arginine, 5 g of ectoine, 5 g of ergothioneine, 5 g of ascorbyl tetraisopalmitate, 30 g of p-hydroxyacetophenone, 30 g of 1,2-butanediol, 30 g of polydimethylsiloxane, 40 g of hydrogenated rapeseed oil alcohol, 30 g of C12-20 alkyl glucoside, 5 g of dipeptide diamino butyryl benzylamide diacetate, and 10 g of sodium chloride, and perform homogenization at 55°C and 8000 rpm for 5 min, then keep warm for 8 min to obtain a viscous composition;

[0082] Step 5: Cool the viscous composition obtained in Step 4 to room temperature by stirring at 100 rpm, pass it through a 200-mesh sieve and discharge it to obtain the moisturizing and anti-wrinkle eye cream.

[0083] The preparation method of the modified plantain fruit extract is as follows:

[0084] Dissolve 0.8 g of polyvinylpyrrolidone, 0.5 g of 4-dimethylaminopyridine, 0.8 mL of triethylamine and 35 mg of plantain fruit extract in 40 mL of a 2% sodium sulfate aqueous solution, stir and react for 24 h under the conditions of light avoidance and nitrogen protection, with a stirring speed of 800 rpm. After the reaction is completed, transfer all the mixed solution into a dialysis bag, immerse the dialysis bag in double-distilled water, and dialyze for 3 d; then add 16 mg of 1-hydroxy-2-naphthoic acid to it, heat to 80°C, stir and react for 20 min, then centrifuge to obtain the precipitate, and perform vacuum freeze-drying to prepare the modified plantain fruit extract.

[0085] Example 4

[0086] A preparation method of a moisturizing and anti-wrinkle eye cream, the steps are as follows:

[0087] Step 1: Stir 400 g of water, 40 g of glycerol, 20 g of 1,2-butanediol, 30 g of 1,3-propanediol, 40 g of acrylate / C10-30 alkyl acrylate cross-linked polymer, 10 g of sodium hyaluronate, 50 g of allantoin, 30 g of trehalose, and 3 g of phosphatidylcholine at 80°C and 100 rpm for 15 min to obtain the A component;

[0088] Step 2: Mix 40 g of tocopheryl acetate, 2 g of sunflower seed oil, 5 g of phospholipids, 40 g of triheptanoin, and 30 g of C14 - 22 alcohol at 80°C and 100 rpm for 15 min to obtain Component B;

[0089] Step 3: Add the Component B obtained in Step 2 to the Component A obtained in Step 1, and carry out isothermal homogenization at 80°C and 8000 rpm for 8 min to obtain a homogenized mixture;

[0090] Step 4: Cool the homogenized mixture obtained in Step 3 to 55°C by stirring at 100 rpm, and add 50 g of modified plantain fruit extract, 5 g of reed extract, 5 g of licorice root extract, 4 g of angelica root extract, 6 g of salvia root extract, 10 g of cornel fruit extract, 10 g of wolfberry root extract, 40 g of glucosyl hesperidin, 10 g of tocopherol, 30 g of arginine, 5 g of ectoine, 5 g of ergothioneine, 5 g of ascorbyl tetraisopalmitate, 30 g of p - hydroxyacetophenone, 30 g of 1,2 - butanediol, 30 g of polydimethylsiloxane, 40 g of hydrogenated rapeseed oil alcohol, 30 g of C12 - 20 alkyl glucoside, 5 g of dipeptide diamino butyryl benzyl amide diacetate, and 10 g of sodium chloride. Homogenize at 55°C and 8000 rpm for 5 min and keep warm for 8 min to obtain a viscous composition;

[0091] Step 5: Stir and cool the viscous composition obtained in Step 4 to room temperature at 100 rpm, pass through a 200 - mesh sieve, and discharge to obtain the moisturizing and anti - wrinkle eye cream.

[0092] The preparation method of the modified plantain fruit extract is as follows:

[0093] Dissolve 0.8 g of polyvinylpyrrolidone, 0.5 g of 4 - dimethylaminopyridine, 0.8 mL of triethylamine, and 35 mg of plantain fruit extract in 40 mL of 2% sodium sulfate aqueous solution. Stir and react for 24 h under the conditions of light avoidance and nitrogen protection, with a stirring speed of 800 rpm. After the reaction, transfer all the mixed solution into a dialysis bag, immerse the dialysis bag in double - distilled water, and dialyze for 3 d; then add 16 mg of ethyl p - aminobenzoate thereto, heat to 80°C, stir and react for 20 min, then centrifuge to obtain the precipitate, and carry out vacuum freeze - drying to prepare the modified plantain fruit extract.

[0094] Example 5

[0095] A preparation method of a moisturizing and anti - wrinkle eye cream, the steps are as follows:

[0096] Step 1: Stir 400 g of water, 40 g of glycerol, 20 g of 1,2 - butanediol, 30 g of 1,3 - propanediol, 40 g of acrylate / C10 - 30 alkyl acrylate cross - polymer, 10 g of sodium hyaluronate, 50 g of allantoin, 30 g of trehalose, and 3 g of phosphatidylcholine at 80 °C and 100 rpm for 15 min to obtain Component A;

[0097] Step 2: Stir 40 g of tocopheryl acetate, 2 g of sunflower seed oil, 5 g of phospholipids, 40 g of triheptanoin, and 30 g of C14 - 22 alcohol at 80 °C and 100 rpm for 15 min to obtain Component B;

[0098] Step 3: Add the Component B obtained in Step 2 to the Component A obtained in Step 1, and perform isothermal homogenization at 80 °C and 8000 rpm for 8 min to obtain a homogenized mixture;

[0099] Step 4: Stir the homogenized mixture obtained in Step 3 at 100 rpm to cool down to 55 °C, and add 50 g of modified plantain fruit extract, 5 g of reed extract, 5 g of licorice root extract, 4 g of angelica root extract, 6 g of salvia root extract, 10 g of cornel fruit extract, 10 g of wolfberry root extract, 40 g of glucosyl hesperidin, 10 g of tocopherol, 30 g of arginine, 5 g of ectoine, 5 g of ergothioneine, 5 g of tetra - isopalmitoyl ascorbate, 30 g of p - hydroxyacetophenone, 30 g of 1,2 - butanediol, 30 g of polydimethylsiloxane, 40 g of hydrogenated rapeseed oil alcohol, 30 g of C12 - 20 alkyl glucoside, 5 g of dipeptide diamino butyryl benzyl amide diacetate, and 10 g of sodium chloride. Homogenize at 55 °C and 8000 rpm for 5 min and keep warm for 8 min to obtain a viscous composition;

[0100] Step 5: Stir and cool the viscous composition obtained in Step 4 to room temperature at 100 rpm, pass it through a 200 - mesh sieve, and discharge to obtain the moisturizing and anti - wrinkle eye cream.

[0101] The preparation method of the modified plantain fruit extract is as follows:

[0102] Dissolve 0.8 g of polyvinylpyrrolidone, 0.5 g of 4 - dimethylaminopyridine, 0.8 mL of triethylamine, and 35 mg of plantain fruit extract in 40 mL of 2% sodium sulfate aqueous solution. Stir and react for 24 h under the conditions of light avoidance and nitrogen protection, with a stirring speed of 800 rpm. After the reaction, transfer all the mixed liquid into a dialysis bag, immerse the dialysis bag in double - distilled water, and dialyze for 3 d; then add 16 mg of methyl salicylate to it, heat to 80 °C, stir and react for 20 min, then centrifuge to obtain the precipitate, and perform vacuum freeze - drying to prepare the modified plantain fruit extract.

[0103] Example 6

[0104] A preparation method of a moisturizing and anti-wrinkle eye cream comprises the following steps:

[0105] Step 1: Stir 400 g of water, 40 g of glycerol, 20 g of 1,2-butanediol, 30 g of 1,3-propanediol, 40 g of acrylate / C10-30 alkyl acrylate cross-linked polymer, 10 g of sodium hyaluronate, 50 g of allantoin, 30 g of trehalose, and 3 g of phosphatidylcholine at 80 °C and 100 rpm for 15 min to obtain Component A;

[0106] Step 2: Stir 40 g of tocopheryl acetate, 2 g of sunflower seed oil, 5 g of phospholipids, 40 g of triheptanoin, and 30 g of C14-22 alcohol at 80 °C and 100 rpm for 15 min to obtain Component B;

[0107] Step 3: Add the Component B obtained in Step 2 to the Component A obtained in Step 1, and carry out constant-temperature homogenization at 80 °C and 8000 rpm for 8 min to obtain a homogenized mixture;

[0108] Step 4: Cool the homogenized mixture obtained in Step 3 to 55 °C while stirring at 100 rpm, and add 50 g of modified banana fruit extract, 5 g of reed extract, 5 g of licorice root extract, 4 g of angelica root extract, 6 g of salvia root extract, 10 g of cornel fruit extract, 10 g of wolfberry root extract, 40 g of glucosyl hesperidin, 10 g of tocopherol, 30 g of arginine, 5 g of ectoine, 5 g of ergothioneine, 5 g of tetrahexyldecyl ascorbate, 30 g of p-hydroxyacetophenone, 30 g of 1,2-butanediol, 30 g of polydimethylsiloxane, 40 g of hydrogenated rapeseed oil alcohol, 30 g of C12-20 alkyl glucoside, 5 g of dipeptide diaminobutyryl benzylamide diacetate, and 10 g of sodium chloride, and carry out homogenization at 55 °C and 8000 rpm for 5 min and keep warm for 8 min to obtain a viscous composition;

[0109] Step 5: Cool the viscous composition obtained in Step 4 to room temperature while stirring at 100 rpm, pass through a 200-mesh sieve, and discharge to obtain the moisturizing and anti-wrinkle eye cream.

[0110] The preparation method of the modified banana fruit extract is as follows:

[0111] Dissolve 0.8 g of polyvinylpyrrolidone, 0.5 g of 4-dimethylaminopyridine, 0.8 mL of triethylamine and 35 mg of banana fruit extract in 40 mL of a 2% sodium sulfate aqueous solution, stir and react for 24 h under the conditions of light avoidance and nitrogen protection, with a stirring speed of 800 rpm. After the reaction, transfer all the mixed solution into a dialysis bag, immerse the dialysis bag in double-distilled water, and dialyze for 3 d; then add 16 mg of methyl cinnamate thereto, heat to 80 °C, stir and react for 20 min, then centrifuge to obtain the precipitate, and freeze-dry under vacuum to prepare the modified banana fruit extract.

[0112] Test Example 1: Safety Test

[0113] (1) Erythrocyte Hemolysis Experiment

[0114] Preparation of erythrocyte suspension: Select healthy rabbits, draw 9 mL of blood from the heart, add 1 mL of 2% potassium oxalate solution, centrifuge, discard the supernatant, dilute the precipitate to 20 mL with 20 mmol / L PBS solution, and store at 4 °C for later use. Dilute the samples to different concentrations with PBS solution, and set 5 concentration gradients for each sample. Take 10 mL of the diluted solution of the sample to be tested, add 200 μL of the above-mentioned erythrocyte suspension (control the final concentrations of the samples to be 5, 10, 20, 50, 100 mg / mL), use distilled water as the total hemolysis control, and PBS solution as the negative control, gently mix well, incubate at 37 °C for 30 min, centrifuge at a speed of 2000 r / min for 10 min, take the supernatant, measure its absorbance (A560) at 560 nm with a spectrophotometer, and calculate the hemolysis rate according to the following formula;

[0115] Hemolysis rate = [(A560 of the experimental group - A560 of the negative control group) / (A560 of the total hemolysis control group - A560 of the negative control group)] × 100%

[0116] Draw a standard curve of hemolysis rate - sample concentration, and calculate the sample concentration (HD50) when 50% of the erythrocytes are hemolyzed.

[0117] (2) Protein Denaturation Test

[0118] Dilute the sample to 10 g / L with PBS solution, take 10 mL of the diluted solution of the sample to be tested, add 200 μL of the above-mentioned erythrocyte suspension, use distilled water as the blank control, and 1 mg / mL sodium dodecyl sulfate (SDS) solution as the positive control, gently mix well, incubate at 37 °C for 30 min, centrifuge at a speed of 2000 r / min for 10 min, take the supernatant, measure its absorbances A540 and A575 at 540 nm and 575 nm respectively with a spectrophotometer, and calculate the protein denaturation index (DI) according to the following formula;

[0119] DI = [(R1 - R2) / (R1 - R3)] × 100%

[0120] Among them, R1 = A575 of the blank control group / A540 of the blank control group, R2 = A575 of the experimental group / A540 of the experimental group, R3 = A575 of the positive control group / A540 of the positive control group

[0121] Evaluate the irritation of the sample to be tested according to the L / D value, where the value of L / D is HD50 / DI, and the irritation classification standard of the red blood cell hemolysis test is shown in Table 1 below:

[0122] Table 1 Irritation classification standard of red blood cell hemolysis test

[0123]

[0124]

[0125] Table 2 Test results of red blood cell hemolysis and protein denaturation tests

[0126] Test sample <![CDATA[HD 50 (mg / L)]]> DI(%) L / D Evaluation Example 1 14321 16.24 10 < L / D ≤ 100 Slightly irritating Example 2 15791 13.23 ＞100 Non-irritating Example 3 16291 10.31 ＞100 Non-irritating Example 4 15223 14.21 ＞100 Non-irritating Example 5 15336 13.33 ＞100 Non-irritating Example 6 15219 13.14 ＞100 Non-irritating

[0127] It can be seen from the safety performance test that the L / D of the moisturizing and anti-wrinkle eye creams provided in Examples 2 - 6 is greater than 100, and the irritation classification is non-irritating, indicating that the moisturizing and anti-wrinkle eye creams provided in Examples 2 - 6 have the characteristics of being mild and non-irritating. This shows that the product of the plantain fruit extract modified by nano-capsulation has less irritation and is mild and safe.

[0128] It can be seen from Table 2 above that when comparing Examples 1 - 6, the sample concentration HD 50 (mg / L) when 50% of the red blood cells in Example 3 are hemolyzed is the largest, and the sample concentration HD 50(mg / L) is the lowest; the protein denaturation index DI of Example 3 is the lowest, and the protein denaturation index DI of Example 1 is the highest. The possible reasons are as follows: 1. The banana fruit extract is modified by polyvinylpyrrolidone to form nano-capsules. These nano-capsules can enable the moisturizing factor to penetrate the stratum corneum more deeply and reach the deep layer of the skin, thereby improving the moisturizing effect. Polyvinylpyrrolidone contains amino and carbonyl functional groups, and these functional groups may have some biocompatibility and anti-inflammatory properties, which may help reduce the irritation and mildness of the eye cream to the skin. The banana fruit extract is modified by polyvinylpyrrolidone to form nano-capsules, which may help improve the stability of the components and reduce the irritating odor of the composition. In addition, the nano-capsules can control the release rate of the composition, extend the action time, and thus improve the action effect. The amino and carbonyl functional groups in polyvinylpyrrolidone may help reduce the irritation and mildness of the eye cream; 2. 1-Hydroxy-2-naphthoic acid contains two connected benzene rings, and one of the benzene rings is directly connected with a hydroxyl group and a carboxyl group. This structure makes 1-hydroxy-2-naphthoic acid have high ultraviolet absorption performance and good antibacterial performance. By connecting 1-hydroxy-2-naphthoic acid to the outer surface of the polyvinylpyrrolidone nano-capsules, the sun protection and antibacterial effects of the eye cream can be further improved. At the same time, the amino and carbonyl functional groups in polyvinylpyrrolidone and the benzene ring structure of 1-hydroxy-2-naphthoic acid may act together to reduce the irritation and mildness of the eye cream.

[0129] For Examples 4-6, the sample concentration HD when 50% of red blood cells hemolyze 50 (mg / L) is larger than that of Example 1, but the protein denaturation index DI is smaller than that of Example 1, which indicates that the banana fruit extract modified by ethyl p-aminobenzoate, methyl salicylate, and methyl cinnamate can also deeply repair and moisten the skin.

[0130] Through the above safety tests, it can be obtained that in terms of product safety, Example 3 is the best example of the present invention, and it also shows that the present invention meets the safety requirements of compound cosmetics.

[0131] Test Example 2: Moisturizing performance test

[0132] (1) Moisturizing performance test by in vitro weighing method

[0133] Weigh 0.2 g of the moisturizing and anti-wrinkle eye cream prepared in the above examples and comparative examples, evenly apply them on a 5 cm × 5 cm glass plate pasted with microporous ventilation tape, and place the glass plate in a constant temperature and humidity dryer. Weigh the mass of the glass plate after 4 h respectively, and calculate its moisturizing rate. The formula for calculating the moisturizing rate is:

[0134] Moisturizing rate / % = (M2 - M0) / (M1 - M0) × 100%

[0135] Among them, M0 is the mass of the glass plate in grams, M1 is the mass of the glass plate after adding the sample in grams, and M2 is the mass of the glass plate after being placed in the desiccator for several hours in grams.

[0136] (2) Transdermal water loss change rate

[0137] Sixty skin-healthy volunteers aged 18 - 40 were selected, with an equal male-female ratio. They were randomly divided into 6 groups, with 5 males and 5 females in each group, corresponding to Examples 1 - 6 respectively. The testing instrument was the Tewameter TM 300 from CK Company, Germany. The testing external environment was: room temperature 25°C, humidity 60%.

[0138] Before using the skin composition and after continuous use for 4 days, the transdermal water loss change rate of each subject was tested. Each data was tested three times. After taking the average value, the average value of each group was calculated and reserved to one decimal place.

[0139] (3) Moisture retention rate

[0140] Sixty skin-healthy volunteers aged 18 - 40 were selected, with an equal male-female ratio. They were randomly divided into 6 groups, with 5 males and 5 females in each group, and Examples 1 - 6 were applied respectively. The instrument was the Derma Unit SSC of the model from Courage+Khazaka electronic, Germany. The testing temperature was 25°C and the humidity was 60%.

[0141] Before use and 4 hours after use, the skin moisture content of each subject was tested. Each data was tested three times. After taking the average value, the average value of each group was calculated and reserved to one decimal place. The test results are shown in Table 3.

[0142] Table 3 Test results of moisturizing performance

[0143] Test sample Moisture retention rate / % Rate of change in transepidermal water loss / % Skin moisture content / % Example 1 41.2 13.5 32.1 Example 2 52.5 15.3 42.5 Example 3 82.5 18.5 67.5 Example 4 63.5 20.5 50.3 Example 5 66.1 19.8 51.5 Example 6 62.3 21.6 52.3

[0144] From the data in Table 3 above, it can be seen that the anti-wrinkle eye cream provided by the present invention has a good moisturizing effect. Its moisturizing rate is 41.2 - 82.5%, the transdermal water loss change rate is relatively small, being 13.5 - 21.6%, and the skin moisture content after using for 4 hours is 32.1 - 67.5%. This shows that the skin can remain moist after use, which helps to fade dry lines.

[0145] Comparing Examples 1-6, it was found that in Example 3, the moisture retention rate and the skin moisture content after 4 hours of use were the highest, and the moisturizing effect was the best. The possible reason is that a stable emulsion was prepared by encapsulating with nano-sized capsule particles, which improved the stability of the components, reduced the pungent odor of the composition, controlled the release rate of the composition, extended the action time, and enhanced the action effect. Making the moisturizing and anti-wrinkle components into nano-particle size can maximize their efficacy. The moisturizing and anti-wrinkle eye cream of the present invention can penetrate through the stratum corneum to reach the deep layer of the skin, not only moisturizing deeply but also moisturizing persistently.

[0146] Test Example 4: Resistance to ultraviolet rays test

[0147] The sun protection performance (SPF value) of the prepared moisturizing and anti-wrinkle eye cream was measured by an ultraviolet transmittance analyzer.

[0148] The UV-2000S ultraviolet transmittance analyzer of Labsphere, Inc. of the United States was used to test the sunscreen cream. First, the test substrate was weighed and tared on a measuring balance; the sample was dropped onto the PMMA plate test substrate, and the sample was spotted at 9 points on the test substrate, and the spotting amount of the 9 points was made as equal as possible, with a total loading amount of 32 mg. Then, wear gloves and stretch the glove at the finger to make the glove flat at the finger, ensuring the smoothness of the finger contacting the test substrate during application; after evenly applying the 9 points, then evenly apply the entire substrate; after application, weigh to ensure that the remaining amount of the sample on the test substrate is between 22 and 26 mg; then place the test substrate horizontally in a dark place at room temperature to dry and balance for 20 min; after the test sample dries and forms a film on the test substrate, it is placed in an ultraviolet transmittance analyzer for ultraviolet transmittance scanning test under ultraviolet irradiation in the wavelength range of 290 nm to 400 nm. Five different positions are tested on each test substrate, and then the scanning data of the 5 positions are averaged. Five test substrates are applied for each sample, and the average value is taken to obtain the SPF of the sample.

[0149] The test samples were respectively Examples 1-6 of the present invention. The above test samples were all stored for 10 months under the condition of standing in a brown glass container at 25°C.

[0150] Take human epidermal cells HEK-n in the logarithmic growth phase, at a concentration of 1×10 5Cells were inoculated into a 96-well plate at a concentration of cells / mL, with a volume of 200 μL per well. After normal culture for 24 h, in the cell culture medium, the moisturizing and anti-wrinkle eye creams prepared in Examples 1-6 were added respectively. After 5 min, oxidative damage treatment was carried out with 1 mM H2O2 for 6 h. After the oxidative damage treatment was completed, the 96-well culture plate was taken out, the supernatant was discarded, and after carefully rinsing 2-3 times with PBS, 200 μL of fresh culture medium containing 0.5 mg / mL MTT was added, and the culture was continued at 37 °C and 5% CO2 for 4 h. After the culture was completed, the MTT solution was discarded, 100 μL of an aqueous sodium sulfate solution with a mass fraction of 2.0% was added to each well, shaken and mixed evenly, the absorbance value (A490) of each well at a wavelength of 490 nm was measured, and the cell survival rate was calculated. The results are shown in Table 4.

[0151] Table 4. Testing of the SPF value and cell survival rate of the moisturizing and anti-wrinkle eye creams of each example

[0152] Test sample SPF value Cell survival rate (%) Example 1 28.6 42.1 Example 2 30.1 43.6 Example 3 65.6 85.5 Example 4 41.3 65.3 Example 5 42.5 61.2 Example 6 39.8 64.8

[0153] According to Table 4 above, by comparing Examples 1-3, it can be seen that the SPF value of the group of Example 3 is the largest and the cell survival rate is the highest. The possible reasons are as follows: 1. Enhancing the ultraviolet absorption ability: Some nanomaterials contained in the modified nanocapsules of plantain fruit extract can absorb and reflect sunlight, especially ultraviolet light, thus reducing the damage of ultraviolet rays to the skin; 2. Improving the light stability: The modified plantain fruit extract can inhibit or reduce the photosensitive components in cosmetics, thus improving the light stability of cosmetics and preventing the product from decomposing or discoloring in the sun; 3. Enhancing the permeability: The size of the nanocapsules enables them to penetrate the stratum corneum on the skin surface, and the modified plantain fruit extract directly brings the sunscreen components to the deep layer of the skin, thus improving the sunscreen effect; 4. Reducing skin irritation: The nanocapsules can act as a physical barrier to help the skin resist the harmful rays in the sun, reduce the damage of ultraviolet rays to skin cells, and thus reduce skin irritation and inflammation; 5. Improving the moisturizing property: The nanocapsules can also act as a carrier for moisturizing agents, evenly distributing the moisturizing components in the cosmetics, making the product more moisturizing and improving the use feeling; with less influence of ultraviolet rays, the cell survival rate naturally increases.

[0154] Test Example 5: Stability test

[0155] To investigate the pH stability and color stability of the cosmetic compositions of Examples 1-6, first, the initial pH of each cosmetic composition was tested and the initial values were recorded; subsequently, each cosmetic composition was sub-packed and placed at room temperature, on the window sill, under light (25 °C, visible light intensity 16000 LX), high temperature (48 °C and 60 °C) for 2 and 4 weeks to investigate the pH change and color change of the composition under different conditions, and the appearance after 4 weeks was photographed. The results are shown in Table 5 below.

[0156] Table 5 Stability (pH, Color) Test of Moisturizing and Anti-Wrinkle Eye Cream in Each Example

[0157]

[0158]

[0159] From Table 5 above, by comparing Examples 1-6, it can be seen that the moisturizing and anti-wrinkle eye cream prepared in Example 3 has relatively stable chemical properties. The possible reasons are as follows: 1. Absorption effect: 1-Hydroxy-2-naphthoic acid is a compound with a specific structure. Chemically connecting it to polyvinylpyrrolidone makes the whole have the function of absorbing ultraviolet light. When it is connected to polyvinylpyrrolidone, this protective layer can absorb ultraviolet light, thereby reducing the destructive effect of ultraviolet light on other components in the cosmetic and preventing color change; 2. Chemical stabilization effect: 4-Hydroxyacetophenone may undergo oxidation or degradation reactions under certain conditions, resulting in color change. By connecting 1-hydroxy-2-naphthoic acid to polyvinylpyrrolidone to form a protective layer, it can inhibit the direct contact of 4-hydroxyacetophenone with external factors, thereby reducing its degradation rate and maintaining the stability of the cosmetic; 3. Anti-photoaging effect: 1-Hydroxy-2-naphthoic acid itself has antibacterial and antioxidant properties, which can resist the damage of ultraviolet light to the skin and prevent photoaging. When it is connected to polyvinylpyrrolidone to form a protective layer, this anti-photoaging effect is maintained and transmitted to the entire cosmetic system, thereby extending the shelf life of the cosmetic; Formation of chemical bonds: 1-Hydroxy-2-naphthoic acid contains phenolic hydroxyl and carboxyl groups, and polyvinylpyrrolidone contains carbonyl and amino groups. Through chemical reactions, these groups can form ester bonds or amide bonds, so that 1-hydroxy-2-naphthoic acid is firmly connected to polyvinylpyrrolidone to form a stable protective layer; 4. Encapsulation of 4-hydroxyacetophenone: Since polyvinylpyrrolidone can form nanocapsules, 4-hydroxyacetophenone can be encapsulated in these nanocapsules. By connecting 1-hydroxy-2-naphthoic acid to polyvinylpyrrolidone to form a protective layer, the encapsulation effect of 4-hydroxyacetophenone can be further enhanced, isolating it from external factors, thereby maintaining the stability of the cosmetic; 5. Acid-base neutralization: 1-Hydroxy-2-naphthoic acid contains phenolic hydroxyl and carboxyl groups and is acidic; 4-hydroxyacetophenone contains amino groups and is basic. By connecting 1-hydroxy-2-naphthoic acid to polyvinylpyrrolidone, an acid-base neutralization balance state may be formed, reducing the degradation rate of 4-hydroxyacetophenone, thereby maintaining the stability of the cosmetic; 6. Steric hindrance effect: By connecting 1-hydroxy-2-naphthoic acid to polyvinylpyrrolidone to form a protective layer, a certain steric hindrance effect may be generated, that is, preventing 4-hydroxyacetophenone from directly contacting external factors and reducing the probability of its oxidation, degradation and other reactions. This steric hindrance effect can reduce the speed at which 4-hydroxyacetophenone changes the color of the cosmetic.

[0160] The possible reasons why Example 3 is superior to Examples 4 - 6 are as follows: 1. By connecting 1-hydroxy-2-naphthoic acid to polyvinylpyrrolidone, a balanced state of acid-base neutralization may be formed, reducing the degradation rate of p-hydroxyacetophenone and thus maintaining the stability of the cosmetic; 2. The unique double benzene ring structure of 1-hydroxy-2-naphthoic acid itself increases the molecular size in a certain direction, thereby restricting the interaction of other molecules or groups with this reactive group. The generation of steric hindrance effect is closely related to the size and shape of the molecular structure and the intermolecular interaction. 1-Hydroxy-2-naphthoic acid may produce obvious steric hindrance due to its large structural size, while ethyl p-aminobenzoate, methyl salicylate and methyl cinnamate do not produce obvious steric hindrance effect because of their small structures or different binding modes with polyvinylpyrrolidone;

[0161] The above are only the preferred embodiments of the present invention and are not used to limit the present invention. Modifications, substitutions, and improvements made within the principles of the present invention shall all be included within the protection scope of the present invention.

Claims

1. A method for preparing a moisturizing and anti-wrinkle eye cream, characterized in that: The method comprises the following steps, in parts by weight: Step 1: Heat and stir 30-50 parts of water, 4-6 parts of glycerin, 2-5 parts of 1,2-butylene glycol, 3-5 parts of 1,3-propylene glycol, 3-5 parts of acrylic acid (esters) / C10-30 alkyl acrylate cross-linked polymer, 0.5-1 part of sodium hyaluronate, 2-7 parts of allantoin, 2-4 parts of trehalose, and 0.3-0.5 parts of phosphatidylcholine to obtain component A; Step 2: Heat and stir 3-7 parts of tocopherol acetate, 0.1-0.5 parts of sunflower seed oil, 0.3-0.5 parts of phospholipids, 3-5 parts of triheptyl alcohol, and 2-5 parts of C14-22 alcohol to obtain component B; Step 3: Add component B obtained in step 2 to component A obtained in step 1 and homogenize at a constant temperature to obtain a homogeneous mixture; Step 4: Cool the homogenized mixture obtained in step 3 to 55-60°C, add 3-8 parts of banana fruit extract, 0.3-0.5 parts of reed extract, 0.1-1 parts of liquorice root extract, 0.1-0.5 parts of angelica root extract, 0.3-1 parts of salvia root extract, 0.5-2 parts of cornus fruit extract, 0.5-2 parts of wolfberry root extract, 3-5 parts of glucosyl hesperidin, 0.5-1 parts of tocopherol, 3-5 parts of arginine, 0.3-0. 5 parts of tetrahydromethylpyrimidine carboxylic acid, 0.1-0.5 parts of ergothioneine, 0.5-0.8 parts of ascorbyl tetraisopalmitate, 3-5 parts of p-hydroxyacetophenone, 2-5 parts of 1,2-butylene glycol, 3-5 parts of polydimethylsiloxane, 3-5 parts of hydrogenated rapeseed alcohol, 2-5 parts of C12-20 alkyl glucoside, 0.5-1 parts of dipeptide diaminobutyryl benzylamide diacetate, 1-2 parts of sodium chloride, and homogenizing at a constant temperature for 3-5 minutes to obtain a viscous composition; Step 5: Cool the viscous composition obtained in step 4 to room temperature, sieve and discharge the composition to obtain the moisturizing and anti-wrinkle eye cream.

2. The method for preparing the moisturizing and anti-wrinkle eye cream according to claim 1, characterized in that: In the step 1, the heating, stirring and mixing uniformly specifically refers to stirring and mixing at 80-85° C. and 50-200 rpm for 10-20 min; in the step 2, the heating, stirring and mixing uniformly specifically refers to stirring and mixing at 75-80° C. and 50-200 rpm for 10-20 min.

3. The method for preparing the moisturizing and anti-wrinkle eye cream according to claim 1, characterized in that: The constant temperature homogenization in step 3 refers to homogenization at 80-85° C. and 8000-10000 rpm for 5-10 minutes.

4. The method for preparing the moisturizing and anti-wrinkle eye cream according to claim 1, characterized in that: The constant temperature homogenization in step 4 refers to homogenization at 55-60° C. and 8000-10000 rpm for 3-5 minutes.

5. The method for preparing the moisturizing and anti-wrinkle eye cream according to claim 1, characterized in that: Cooling to room temperature in step 5 refers to stirring and cooling to room temperature at 50-200 rpm.

6. The method for preparing the moisturizing and anti-wrinkle eye cream according to claim 1, characterized in that: The step 4 can also be: cooling the homogenous mixture obtained in step 3 to 55-60°C, adding 3-8 parts of modified banana fruit extract, 0.3-0.5 parts of reed extract, 0.1-1 parts of liquorice root extract, 0.1-0.5 parts of angelica root extract, 0.3-1 parts of salvia root extract, 0.5-2 parts of cornus fruit extract, 0.5-2 parts of wolfberry root extract, 3-5 parts of glucosyl hesperidin, 0.5-1 parts of tocopherol, 3-5 parts of arginine, 0. 3-0.5 parts of tetrahydromethylpyrimidine carboxylic acid, 0.1-0.5 parts of ergothioneine, 0.5-0.8 parts of ascorbyl tetraisopalmitate, 3-5 parts of p-hydroxyacetophenone, 2-5 parts of 1,2-butylene glycol, 3-5 parts of polydimethylsiloxane, 3-5 parts of hydrogenated rapeseed alcohol, 2-5 parts of C12-20 alkyl glucoside, 0.5-1 parts of dipeptide diaminobutyryl benzylamide diacetate, 1-2 parts of sodium chloride, and homogenizing at a constant temperature for 3-5 minutes to obtain a viscous composition.

7. The method for preparing the moisturizing and anti-wrinkle eye cream according to claim 6, characterized in that: The preparation steps of the modified banana fruit extract are as follows: 0.6-1.0g polyvinyl pyrrolidone, 0.3-0.5g 4-dimethylaminopyridine, 0.5-0.8mL triethylamine and 30-50mg banana fruit extract are dissolved in 25-40mL sodium sulfate aqueous solution with a mass fraction of 2-3%, stirring and reacting for 22-24h under the conditions of light protection and nitrogen protection, and the stirring speed is 500-800rpm. After the reaction is completed, the mixed solution is transferred to a dialysis bag, the dialysis bag is immersed in double distilled water, dialyzed for 2-3d, and the precipitate is taken by centrifugation and vacuum freeze-dried to obtain the modified banana fruit extract.

8. The method for preparing the moisturizing and anti-wrinkle eye cream according to claim 6, characterized in that: The preparation steps of the modified banana fruit extract are as follows: 0.6-1.0g polyvinyl pyrrolidone, 0.3-0.5g 4-dimethylaminopyridine, 0.5-0.8mL triethylamine and 30-50mg banana fruit extract are dissolved in 25-40mL sodium sulfate aqueous solution with a mass fraction of 2-3%, stirring and reacting for 22-24h under the conditions of light protection and nitrogen protection, and the stirring speed is 500-800rpm. After the reaction is completed, the mixed solution is transferred to a dialysis bag, the dialysis bag is immersed in double distilled water, and dialyzed for 2-3d, and then 13-16mg of a modifier is added thereto, heated to 80-90°C, and stirred for reaction for 20-30min; centrifugation is performed to obtain a precipitate, vacuum freeze-dried, and the modified banana fruit extract is obtained.

9. The method for preparing the moisturizing and anti-wrinkle eye cream according to claim 8, characterized in that: The modifier is any one of 1-hydroxy-2-naphthoic acid, ethyl p-aminobenzoate, methyl salicylate and methyl cinnamate.

10. A moisturizing and anti-wrinkle eye cream, characterized in that: Prepared by the method according to any one of claims 1 to 9.

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