Fluid supply device and gene sequencer

Abstract

The invention provides a fluid supply device and a gene sequencer, and the fluid supply device comprises a buffer solution box which is used for storing a buffer solution; the kit is positioned on the upper layer of the buffer solution box and is used for storing a reagent; the reagent needles are provided with long reagent needles and short reagent needles, the long reagent needles are used for sucking the buffer solution in the buffer solution box, and the short reagent needles are used for sucking reagents in the kit; the lifting assembly is used for carrying the reagent needle to lift; and the pipeline is communicated with the outlet of the reagent needle and is used for conveying the buffer solution and the reagent to the carrying table. Compared with the prior art, the kit and the buffer solution box share one lifting mechanism, and the long reagent needle and the short reagent needle are respectively used for sucking the buffer solution and the reagent, so that the kit and the buffer solution box can be stacked up and down, the structural layout is more compact, the space is saved, and the number of parts is reduced.

Description

Technical Field

[0001] The present invention belongs to the technical field of gene sequencing, and particularly relates to a fluid supply device and a gene sequencer. Background Art

[0002] A gene sequencer is an instrument for determining the base sequence, type, and quantification of DNA fragments. Currently, the main gene sequencing technologies include Sanger sequencing, next-generation sequencing, third-generation sequencing, etc. The structure of a sequencer based on next-generation sequencing technology mainly includes: a fluid supply device for providing reagents and buffers, a fluid system for transporting fluids, a stage, and a detection device. The fluid is transported by the fluid system to a flow cell on the stage and detected by the detection device.

[0003] In existing sequencers, the fluid supply device includes a buffer supply device and a reagent supply device arranged side by side left and right, and each supply device is provided with a set of lifting mechanisms, and the two sets of lifting mechanisms work independently of each other. However, this layout method causes the fluid supply device to occupy a large space, which is not conducive to the miniaturization of the whole machine. Summary of the Invention

[0004] The present application discloses a fluid supply device and a gene sequencer to solve the problem that the fluid supply device in the prior art needs to occupy a large space.

[0005] In the first aspect of the present application, there is provided a fluid supply device, including:

[0006] A buffer cassette for storing buffer;

[0007] A reagent kit located above the buffer cassette for storing reagents;

[0008] A reagent needle provided with a long reagent needle and a short reagent needle, the long reagent needle being used to aspirate the buffer in the buffer cassette, and the short reagent needle being used to aspirate the reagent in the reagent kit;

[0009] A lifting assembly for carrying the reagent needle up and down;

[0010] A pipeline communicated with the outlet of the reagent needle for transporting the buffer and the reagent to the stage, and the stage is used for carrying a chip for performing a sequencing reaction.

[0011] Optionally, it further includes: a buffer chamber and a reagent chamber arranged in a stacked manner, the buffer chamber being used for carrying the buffer cassette, and the reagent chamber being used for carrying the reagent kit.

[0012] Optionally, the buffer cassette is provided with an opening, and the long reagent needle penetrates through the reagent chamber and extends to the opening of the buffer cassette.

[0013] Optionally, the kit is provided with a plurality of reagent positions, each reagent position having a reagent hole, and the short reagent needle extends through the reagent hole to the reagent position.

[0014] Optionally, the reagent cartridge includes a top plate, a bottom plate, and side plates disposed between the top plate and the bottom plate. A plurality of guide holes are formed at positions on the top plate and the bottom plate that are not covered by the kit, and the long reagent needle or the short reagent needle extends downward through the guide holes.

[0015] Optionally, the buffer solution cartridge is slidably disposed at the bottom of the buffer solution chamber; the kit is slidably disposed at the bottom of the reagent cartridge.

[0016] Optionally, the openings of the buffer solution cartridge and the kit face the same side, and the opening direction is consistent with the sliding direction of the buffer solution cartridge and / or the kit;

[0017] The fluid supply device is further provided with a chamber door for covering the openings of the buffer solution cartridge and the kit.

[0018] Optionally, the lifting assembly includes:

[0019] A driving motor;

[0020] A lead screw connected to the driving motor for rotating under the drive of the driving motor;

[0021] A movable plate for driving the long reagent needle and the short reagent needle to lift under the drive of the lead screw;

[0022] A linear guide rail parallel to the axis of the lead screw, provided with a slider, and the slider is fixedly connected to the movable plate.

[0023] Optionally, a refrigeration assembly is disposed on one side of the reagent cartridge.

[0024] In a second aspect of the present application, a gene sequencer is provided, including the fluid supply device provided by any implementation manner of the first aspect.

[0025] In this embodiment, the buffer cartridge and the reagent kit are stacked vertically. When the lifting assembly descends to a certain height, the long reagent needle extends below the liquid level of the buffer stored in the buffer cartridge to aspirate the buffer; the short reagent needle extends below the liquid level of the reagent stored in the reagent kit to aspirate the reagent. The lifting assembly can stay at a specific position according to the needs of sequencing. After the sequencing is completed, the lifting assembly ascends, causing the short reagent needle and the long reagent needle to separate from the reagent kit and the buffer cartridge respectively, so as to replace the reagent kit and the buffer cartridge. Compared with the prior art, in this embodiment, the reagent kit and the buffer cartridge share a set of lifting mechanisms, and the long and short reagent needles are used to aspirate the buffer and the reagent respectively, enabling the reagent kit and the buffer cartridge to be stacked vertically, with a more compact structural layout, saving space and reducing the number of parts. BRIEF DESCRIPTION OF THE DRAWINGS

[0026] Figure 1 is a schematic diagram of the main structure of a fluid supply device provided by an embodiment of the present application;

[0027] Figure 2 is a schematic cross-sectional view of a fluid supply device provided by an embodiment of the present application;

[0028] Figure 3 is a schematic diagram of the structure of a buffer chamber in a fluid supply device provided by an embodiment of the present application;

[0029] Figure 4 is a schematic diagram of the structure of a reagent chamber in a fluid supply device provided by an embodiment of the present application;

[0030] Figure 5 is a schematic diagram of the structure of a lifting assembly in a fluid supply device provided by an embodiment of the present application;

[0031] Figure 6 is a schematic diagram of the structure of a refrigeration assembly in a fluid supply device provided by an embodiment of the present application;

[0032] Figure 7 is an exploded schematic diagram of the refrigeration assembly provided by an embodiment of the present application.

[0033] Reference numerals: 1 - buffer cartridge; 11 - opening; 2 - reagent kit; 21 - reagent position; 211 - reagent well; 3 - reagent needle; 31 - long reagent needle; 32 - short reagent needle; 4 - lifting assembly; 41 - drive motor; 42 - lead screw; 43 - movable plate; 44 - linear guide; 441 - slider; 5 - buffer chamber; 51 - first guide plate; 52 - first limit slider; 6 - reagent chamber; 60 - guide hole; 61 - top plate; 62 - bottom plate; 63 - side plate; 64 - second guide plate; 65 - second limit slider; 7 - chamber door; 8 - refrigeration assembly; 81 - external fan; 82 - heat dissipation fin; 83 - Peltier module; 84 - Peltier gasket; 85 - heat insulation cotton; 86 - fixing plate; 87 - refrigeration fin; 88 - internal fan. Detailed implementation manners

[0034] The technical solutions in the embodiments of the present invention will be clearly and completely described below. Apparently, the described embodiments are only a part of the embodiments of the present invention, rather than all the embodiments. All other embodiments obtained by those of ordinary skill in the art based on the embodiments of the present invention without creative efforts shall fall within the protection scope of the present invention.

[0035] Referring to Figure 1 the structural schematic diagram shown, an embodiment of the present application provides a fluid supply device, including: a buffer cartridge 1 for storing buffer; a reagent kit 2 located above the buffer cartridge 1 for storing reagents; a reagent needle 3 provided with a long reagent needle 31 and a short reagent needle 32, the long reagent needle 31 being used to aspirate the buffer in the buffer cartridge 1, and the short reagent needle 32 being used to aspirate the reagents in the reagent kit 2; a lifting assembly 4 for carrying the reagent needle 3 up and down; a pipeline communicating with the outlet of the reagent needle for transporting the buffer and reagents to a stage for carrying a chip for performing a sequencing reaction.

[0036] During gene sequencing, specific buffer and reaction conditions are required, combined with specific reagents, to ensure the activity of DNA enzymes and the progress of the sequencing reaction. In this embodiment, the buffer cartridge and the reagent kit are stacked vertically. When the lifting assembly descends to a certain height, the long reagent needle extends below the liquid level of the buffer stored in the buffer cartridge to aspirate the buffer; the short reagent needle extends below the liquid level of the reagents stored in the reagent kit to aspirate the reagents. The lifting assembly can stay at a specific position according to the needs of sequencing. After the sequencing is completed, the lifting assembly is lifted, so that the short reagent needle and the long reagent needle are respectively separated from the reagent kit and the buffer cartridge to facilitate the replacement of the reagent kit and the buffer cartridge. Compared with the prior art, in this embodiment, the reagent kit and the buffer cartridge share a set of lifting mechanisms, and the long and short reagent needles are respectively used to aspirate the buffer and reagents, enabling the reagent kit and the buffer cartridge to be stacked vertically, with a more compact structural layout, saving space and reducing the number of parts.

[0037] Since both the buffer cartridge 1 and the reagent kit 2 are consumables, for easy replacement, the buffer cartridge 1 and the reagent kit 2 in this embodiment are independently packaged. The fluid supply device provided in this embodiment further includes: a buffer chamber 5 and a reagent chamber 6 arranged in a stacked manner, the buffer chamber 5 is used to carry the buffer cartridge 1, and the reagent chamber 6 is used to carry the reagent kit 2.

[0038] A common partition is shared between the buffer chamber 5 and the reagent chamber 6. This partition is both the bottom plate of the reagent chamber 6 and the top plate of the buffer chamber 5, which makes the overall structure compact. The height of the buffer chamber 5 is slightly greater than the height of the buffer cartridge 1, and the height of the reagent chamber 6 is slightly greater than the height of the reagent kit 2. That is to say, there is a small gap between the buffer cartridge 1, the reagent kit 2 and their respective positions. On the one hand, it is convenient for pulling out or pushing in during replacement. On the other hand, it saves space as much as possible, making the overall structure compact.

[0039] In this embodiment, in order to ensure that the long reagent needle and the short reagent needle can be used to aspirate buffer and reagent respectively, the long reagent needle and the short reagent needle are placed in separate zones.

[0040] Since different types of reagents are required during the sequencing process, in this embodiment, the reagent kit 2 is provided with several reagent positions 21. Each reagent position 21 can hold the same type of reagent or different types of reagents, which can be adjusted according to needs. Each of the reagent positions 21 has a reagent hole 211, and the short reagent needle 32 passes through the reagent hole 211 and extends to the reagent position 21. In order to aspirate the reagent, the reagent holes 211 for storing the reagent are equipped with a corresponding number of short reagent needles 32. Generally speaking, the area where the short reagent needles 32 are located corresponds to the position of the reagent kit 2. Since the reagent is prone to oxidation when in contact with air, an aluminum film is usually provided on the reagent hole 211, and the tip of the short reagent needle 32 can easily puncture the aluminum film during the downward movement of the lifting assembly 4, thereby aspirating the reagent.

[0041] During the sequencing process, multiple types of buffers are usually used, such as high-salt buffer and low-salt buffer, etc., to maintain the stability of the reaction system, ensure the activity of the enzyme, and prevent the influence of other substances in the sample. In this embodiment, at least three types of small boxes can be stored inside the buffer cartridge 1. Each small box is provided with 1 opening, so the buffer cartridge 1 is provided with multiple openings 11, and the long reagent needle 31 passes through the reagent chamber 6 and extends to the opening of the buffer cartridge 1.

[0042] To facilitate the arrangement of multiple long reagent needles 31, the small boxes inside the buffer cartridge 1 are set to be strip-shaped, and the openings are arranged on the same straight line. In this way, the long reagent needles 31 can be concentrated in a row.

[0043] Further, the opening 11 of the buffer cartridge 1 is offset from the position of the reagent kit 2. For example, the opening of the buffer cartridge 1 is arranged close to the lifting assembly 4. In this case, the long reagent needle 31 is arranged close to the lifting assembly 4, and the area where the short reagent needle 32 is located is farther from the lifting assembly 4 compared to the long reagent needle 31. Theoretically, the opening 11 of the buffer cartridge 1 can also be arranged at one end far from the lifting assembly 4, so that the area where the short reagent needle 32 is located is closer to the lifting assembly 4. Usually, for the sake of ensuring the stability of the overall structure, the opening of the buffer cartridge is arranged close to the lifting assembly 4.

[0044] Referring to Figure 2 the structural schematic diagram shown, the reagent magazine 6 includes a top plate 61, a bottom plate 62, and side plates 63 arranged between the top plate 61 and the bottom plate 62. A plurality of guide holes 60 are opened at positions on the top plate 61 and the bottom plate 62 that are not covered by the reagent kit 2, and the long reagent needle 31 or the short reagent needle 32 extends downward through the guide holes 60.

[0045] As can be seen from the foregoing description, the long reagent needle 31 needs to pass through the top plate 61 and the bottom plate 62 and continue to extend downward to reach the position of the opening 11, while the short reagent needle 32 only needs to pass through the top plate 61 and the aluminum film on the surface of the reagent hole 211 to suck the liquid.

[0046] In this embodiment, guide columns are installed in the guide holes 60. The guide columns are for the movement of the reagent needles and play a role in supporting and orienting the reagent needles. The guide columns include first guide columns arranged on the top plate 61 and second guide columns arranged on the bottom plate 62. Further, the first guide columns can be divided into first-type guide columns for the long reagent needle 31 to pass through and second-type guide columns for the short reagent needle 32 to pass through.

[0047] For the convenience of replacing the buffer cartridge 1 and the reagent kit 2, the buffer cartridge 1 is slidably arranged at the bottom of the buffer magazine 5; the reagent kit 2 is slidably arranged at the bottom of the reagent magazine 6. In this embodiment, the lifting assembly 4 has at least two working positions. When in the first working position, the long reagent needle 31 and the short reagent needle 32 respectively extend to the buffer cartridge 1 and the reagent kit 2 to suck the reagent; when in the second working position, the end of the reagent needle 3 is located above the top plate 61 of the reagent magazine 6 to facilitate the replacement of the buffer cartridge 1 and the reagent kit 2.

[0048] Referring to Figure 3The structural schematic diagram shown. At the bottom of the buffer solution chamber 5, there is a first guiding plate 51. The buffer solution cartridge 1 is provided with a first guiding groove corresponding to the first guiding plate 51, and the first guiding plate 51 is inserted into the guiding groove of the buffer solution cartridge 1. In the direction in which the buffer solution cartridge 1 is pushed in, there is a first limit slider 52, and a micro switch is provided on the first limit slider 52. When the buffer solution cartridge 1 is placed in place, it presses against the micro switch, and this micro switch is used to determine whether the buffer solution cartridge is placed in place. An RFID sensor is installed on the rear plate of the buffer solution chamber 5. After the buffer solution cartridge 1 is placed in place, the RFID sensor can identify the information of the placed buffer solution cartridge 1.

[0049] Referring to Figure 4 The structural schematic diagram shown. At the bottom of the reagent chamber 6, there is a sheet metal bracket, and a second guiding plate 64 is provided on the sheet metal bracket. The reagent kit 2 is provided with a guiding groove corresponding to the second guiding plate 64, and the second guiding plate 64 is inserted into the guiding groove of the reagent kit 2. In the direction in which the reagent kit 2 is pushed in, there is a second limit slider 65, and a micro switch is provided on the second limit slider 65. When the reagent kit 2 is placed in place, it presses against the micro switch, and this micro switch is used to determine whether the reagent kit 2 is placed in place. A holding box is installed at the top of the reagent chamber 6, and an RFID sensor is fixed inside the box. After the reagent kit is placed in place, the RFID sensor identifies the information of the reagent kit.

[0050] Referring to Figure 2 The structural schematic diagram shown. The openings of the buffer solution cartridge 1 and the reagent kit 2 face the same side, and the opening direction is consistent with the sliding direction of the buffer solution cartridge 1 and / or the reagent kit 2.

[0051] The fluid supply device is further provided with a chamber door 7. The buffer solution chamber 5 is located in the lower layer of the whole structure. The buffer solution chamber 5 and the reagent chamber 6 share a chamber door 7. The chamber door 7 is used to cover the openings of the buffer solution cartridge and the reagent kit. When the chamber door is closed, the box body is in a sealed state. The bottom end of the chamber door 7 is hinged to the bottom end of the box body, and the chamber door 7 can be opened by rotating along the bottom end. This design is simple and convenient and has high practicability.

[0052] Referring to Figure 5 The structural schematic diagram shown. The lifting assembly 4 includes: a driving motor 41; a lead screw 42, connected to the driving motor 41 and used to rotate under the drive of the driving motor 41; a movable plate 43, used to carry the long reagent needle 31 and the short reagent needle 32 to lift and lower under the drive of the lead screw 42; a linear guide rail 44, parallel to the axis of the lead screw 42, provided with a slider 441, and the slider 441 is fixedly connected to the movable plate 43. In this embodiment, the slider 441 can be directly connected to the movable plate 43 or can be fixedly connected to the movable plate 43 through an adapter block, and this is not limited here.

[0053] A motor support is fixed on the top plate 61 of the reagent bin 6. The driving motor 41, the lead screw 42, and the linear guide rail 44 are all fixed to the motor support. The driving motor 41 drives the movable plate 43 to move up and down along the linear guide rail 44 through the lead screw 42. At the same time, the linear guide rail 44 provides a guiding function for the movable plate 43. All the reagent needles 3 are fixed on the movable plate 43 and are driven by the movable plate 43 to suck liquid from the reagent kit 2 and the buffer solution box 1. The movable plate 43 is also provided with reinforcing ribs for increasing the rigidity of the movable plate 43.

[0054] In this embodiment, the lifting assembly 4 is further provided with a limit optocoupler fixed on the motor support, and the movable plate 43 is provided with a baffle paired with the optocoupler module. When the movable plate 43 rises to the upper limit position, the baffle blocks the limit optocoupler, the driving motor stops driving, and the movable plate 43 stops rising. At this time, the reagent needle 3 rises to the highest point, and the bin door 7 can be opened to put in or take out the reagent kit and the buffer solution box. The lower limit position of the movable plate 43 depends on the set number of motor movement steps. When the movable plate starts from the upper limit position and finishes the set number of steps to reach the lower limit position, the reagent needle drops to the lowest point, and at this time, the reagent kit and the buffer solution box can be sucked.

[0055] To improve the throughput, the present application is provided with two relatively independent lifting assemblies 4, corresponding to two sets of reagent needles 3, buffer solution boxes 1, and reagent kits 2. The buffer solution and the reagent are transported to the stage through pipelines, and 2 chips are arranged on the stage. The two fluid supply devices respectively provide fluid for the sequencing reaction on one of the chips.

[0056] Refer to Figure 2 As shown in the structural schematic diagram, a refrigeration assembly 8 is arranged on one side of the reagent bin 6 to keep the temperature inside the reagent bin 6 constant. The left and right side plates 63, the top plate 61, the bottom plate 62, and the bin door of the reagent bin 6 are all covered with heat-insulating cotton to play a role in heat insulation and temperature preservation. The bottom of the partition between the buffer solution bin 5 and the reagent bin 6 is pasted with heat-insulating cotton to increase the heat preservation effect of the reagent bin 6 and prevent condensed water from generating on the lower surface of the partition and falling into the buffer solution bin 5.

[0057] In a realizable manner, the refrigeration assembly 8 is located at the rear of the reagent bin 6 and refrigerates the reagent bin 6 by using the Peltier effect.

[0058] Refer to Figure 6 and Figure 7 As shown in the structural schematic diagram, the refrigeration assembly 8 includes: an outer fan 81, a heat dissipation fin 82, a Peltier module 83, a Peltier gasket 84, a heat-insulating cotton 85, a fixing plate 86, a refrigeration fin 87, and an inner fan 88.

[0059] The heat dissipation fins 82 are located outside the chamber, and the refrigeration fins 87 are located inside the chamber. The heat dissipation fins 82 are provided with three square groove areas, and three Peltier modules 83 are respectively placed in the hollowed-out areas. The hot surfaces of the Peltier modules 83 are in close contact with the heat dissipation fins 82; the refrigeration fins 87 are smaller in size and are fixed to the heat dissipation fins 82 by screws, in contact with and pressing the cold surfaces of the Peltier modules 83. Each Peltier module 83 corresponds to a refrigeration fin 87 respectively, and the screw is equipped with a spring to prevent the Peltier from being crushed when tightened; a square Peltier gasket 84 made of rubber is installed around the Peltier to isolate the air and prevent condensate from forming on the side walls of the Peltier. The heat insulation cotton 85 covers the entire heat dissipation fins 82, isolating the heat dissipation fins 82 from the refrigeration fins 87, preventing the relatively hot heat dissipation fins 87 from transferring heat into the incubator, and there is a wire groove for the Peltier leads. The inner fans 88 are respectively fixed to each refrigeration fin 87 by screws. After the air inside the box is inhaled by the inner fans 88, it flows through the refrigeration fins 87 cooled by the Peltier. The air flow flows up and down along the channels of the refrigeration fins 87, and the temperature of the air flow decreases. After being blown into the chamber, it reduces the temperature of the entire chamber. The fixing plate 86 is fixed to the heat dissipation fins 82, and the fixing plate 86 is fixedly connected to the top plate 61, the side plate 63 and the bottom plate 62 of the reagent chamber 6, fixing the entire refrigeration assembly 8 to the rear of the reagent chamber 6.

[0060] The Peltier module works based on the Peltier effect. The Peltier has two sides, one side for refrigeration and the other side for heating, and the direction of refrigeration and heating can be changed at any time by changing the direction of the current. One side of the Peltier module 83 is in close contact with the refrigeration fin 88 inside the chamber, and the other side is in close contact with the heat dissipation fin 82 outside the chamber. When cooling the reagent chamber 6, the side in close contact with the refrigeration fin 88 cools down the fin; the side in close contact with the heat dissipation fin 82 generates heat, and the external fan 81 blows air on the heat dissipation fin 82 to take away the heat in time. If the heat is not taken away in time, it will affect the refrigeration efficiency.

[0061] This embodiment also provides a gene sequencer, including Figure 1 The fluid supply device provided by any one of the implementation manners. The gene sequencer provided by this application has all the technical effects of the above-mentioned fluid supply device, which will not be elaborated here.

[0062] The above are only the embodiments of the present invention and are not used to limit the present invention. Any modifications, equivalent replacements, improvements, etc. made within the spirit and principles of the present invention are included in the scope of the claims of the present invention pending approval.

Claims

1. A fluid supply device, characterized in that, Comprising: A buffer cartridge for storing buffer solution; A reagent kit located on the upper layer of the buffer cartridge for storing reagents; A reagent needle provided with a long reagent needle and a short reagent needle, the long reagent needle being used to aspirate the buffer solution in the buffer cartridge, and the short reagent needle being used to aspirate the reagents in the reagent kit; A lifting assembly for carrying the reagent needle up and down; A pipeline communicating with the outlet of the reagent needle for transporting the buffer solution and reagents to a stage, the stage being used to carry a chip for performing a sequencing reaction.

2. The fluid supply device according to claim 1, characterized in that, It further comprises: A buffer chamber and a reagent chamber arranged in a stacked manner, the buffer chamber being used to carry the buffer cartridge, and the reagent chamber being used to carry the reagent kit.

3. The fluid supply device according to claim 2, wherein, The buffer cartridge is provided with an opening, and the long reagent needle penetrates through the reagent chamber and extends to the opening of the buffer cartridge.

4. A fluid supply device according to claim 2, wherein The reagent kit is provided with several reagent positions, the reagent positions having reagent holes, and the short reagent needle penetrates through the reagent holes and extends to the reagent positions.

5. A fluid supply device according to claim 3 or 4, characterized in that The reagent chamber includes a top plate, a bottom plate, and side plates arranged between the top plate and the bottom plate. Several guiding holes are provided at positions on the top plate and the bottom plate not covered by the reagent kit, and the long reagent needle or the short reagent needle extends downward through the guiding holes.

6. A fluid supply device according to claim 2, characterized in that, The buffer cartridge is slidably arranged at the bottom of the buffer chamber; the reagent kit is slidably arranged at the bottom of the reagent chamber.

7. The fluid supply device according to claim 6, wherein The openings of the buffer cartridge and the reagent kit face the same side, and the opening direction is the same as the sliding direction of the buffer cartridge and / or the reagent kit; The fluid supply device is further provided with a chamber door for covering the openings of the buffer cartridge and the reagent kit.

8. A fluid supply device according to claim 1, characterized in that, The lifting assembly includes: A driving motor; A lead screw connected to the driving motor for rotating under the drive of the driving motor; A movable plate for carrying the long reagent needle and the short reagent needle up and down under the drive of the lead screw; A linear guide rail parallel to the axis of the lead screw, provided with a slider, and the slider is fixedly connected to the movable plate.

9. The fluid supply device according to claim 2, wherein A refrigeration assembly is arranged on one side of the reagent chamber.

10. A gene sequencer, characterized in that, It includes the fluid supply device according to any one of claims 1 to 9.

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