Non-woven fabric chip containing cranberries and inactivated probiotics and preparation method of non-woven fabric chip

Abstract

The invention discloses a non-woven fabric chip containing cranberries and inactivated probiotics. The non-woven fabric chip comprises a non-woven fabric, a composite functional layer, the inactivated probiotics, cranberry powder and an auxiliary agent. The preparation method comprises the following steps: S1, adding carboxymethyl chitosan into ionized water, dissolving, then adding chitosan, and dissolving; s2, sequentially adding inactivated probiotics, cranberry powder and an auxiliary agent into the mixed solution obtained in the step S1, and uniformly stirring; s3, cooling the mixed solution obtained in the step S3 to 20 DEG C to separate out the compound functional layer; s4, non-woven fabric is placed in the solid-liquid mixture obtained in the step S3, the temperature is reduced to 10 DEG C, and the temperature is kept for 1-2 hours; and S5, taking out the non-woven fabric obtained in the step S4, treating the non-woven fabric by adopting a dipping-rolling process, and slitting the non-woven fabric to obtain a product. The method for preparing the non-woven fabric chip containing cranberries and inactivated probiotics is simple and easy to apply, and the prepared chip is applied to female hygienic products, wound dressing, baby diaper surface layers or mask liners.

Description

Technical Field

[0001] The present invention relates to the technical field of non-woven fabric chips, and specifically to a non-woven fabric chip containing cranberries and inactivated probiotics and a preparation method thereof. Background Art

[0002] Cranberries are evergreen shrubs of the genus Vaccinium in the Ericaceae family, originally native to the cold wetlands of North America. Its name comes from the shape of its flowers resembling a crane's head. Its main uses are as follows: (1) Urinary system health: Cranberries can prevent urinary tract infections, inhibit the fimbrial adhesin proteins of pathogens such as Escherichia coli, and prevent their colonization on the urethral mucosa. (2) Cranberry extract can interfere with the adhesion ability of Helicobacter pylori, reduce the risk of gastric ulcers and gastric cancer, inhibit the binding of bacterial surface adhesins to gastric epithelial cells, regulate the intestinal flora, and promote the proliferation of probiotics such as Bifidobacterium. (3) The synergistic effect of tannic acid and flavonol glycosides in cranberry extract can reduce the oxidation of low-density lipoprotein (LDL) and slow down atherosclerosis. (4) The antioxidant capacity of anthocyanins and vitamin C is 50 times that of vitamin E, which can reduce ultraviolet-induced collagen degradation.

[0003] Currently, the mainstream antibacterial technologies for antibacterial non-woven fabric chips include nano silver, copper-based materials (such as copper oxide), quaternary ammonium salts, and natural antibacterial agents (such as tea polyphenols, lactic acid bacteria extract). Among them, nano silver has become the mainstream choice due to its broad-spectrum antibacterial property (killing rate > 99%); copper oxide has become an emerging direction due to its antiviral ability (such as inhibiting the new coronavirus). Antibacterial non-woven fabric chips load antibacterial agents into non-woven fabric fibers through meltblowing or electrospinning processes to form a "chip-type" functional layer. Its main application fields include: (1) Medical protection: used for surgical gowns, meltblown layers of masks, medical dressings, etc. (2) Hygiene products: products such as sanitary napkins, wet wipes, and pet care pads. (3) Smart home: The demand for products such as antibacterial curtains and mattresses is increasing, including graphene far-infrared antibacterial non-woven fabrics, which have both heat preservation and antibacterial functions.

[0004] In recent years, technological innovations include: (1) Deepening of nanotechnology: Developing nano silver / copper composite antibacterial agents to enhance antibacterial persistence. (2) Rise of bio-based materials: Using biodegradable materials such as polylactic acid (PLA). (3) Smart-responsive chips: Developing temperature-sensitive / pH-responsive antibacterial non-woven fabrics that automatically release antibacterial components according to the environment.

[0005] CN 114892407 B discloses a nano - silver chip and a nano - silver sanitary napkin. The production of the nano - silver chip includes surface treatment of non - woven fabric, impregnation in an antibacterial finishing solution, irradiation under ultraviolet light, steam washing, drying, and cutting steps. The present invention also provides a preparation method of the above - mentioned antibacterial finishing solution. By weight, the antibacterial finishing solution includes 10 - 30 parts of silver nitrate, 120 - 180 parts of cocoa bean shell extract, 60 - 90 parts of water - soluble sophora alopecuroides powder, and 1000 - 2000 parts of water. The present invention uses cocoa bean shells, a by - product discarded in cocoa powder processing, as raw materials, reducing waste and expanding the application field of cocoa bean shells; the cocoa bean shell extract has good antibacterial and antioxidant effects, can reduce silver ions to nano - silver particles, and jointly exerts excellent antibacterial performance with nano - silver particles in the sanitary napkin. However, its performance is not very ideal, and the use of silver ions, which are not natural components, has a large room for improvement. Summary of the Invention

[0006] The object of the present invention is to provide a non - woven fabric chip containing cranberries and inactivated probiotics, which includes non - woven fabric, a composite functional layer, inactivated probiotics, cranberry powder, and an auxiliary agent. The antibacterial peptides released by the inactivated probiotics target and destroy bacterial biofilms, and cranberry proanthocyanidins inhibit bacterial adhesion, avoiding the risk of live bacteria environment. The synergistic effect of cranberry proanthocyanidins and probiotic metabolites reduces the risk of drug resistance, and it is more environmentally friendly than chemical antibacterial agents with a wider application scenario.

[0007] Further, the composite functional layer is chitosan and carboxymethyl chitosan, and the mass ratio of chitosan to carboxymethyl chitosan is 1:2. The composite functional layer forms a three - dimensional cross - linked structure, controlling the slow release of active ingredients, improving the product usage period. The composite functional layer formed by the two not only has a coating effect, but also has a strong adsorption force with the non - woven fabric. At the same time, chitosan itself has the effect of improving immunity, with excellent comprehensive performance.

[0008] Further, the inactivated probiotics are Lactobacillus plantarum or Lactobacillus, and its inactivation method is: inactivate with 280 - nanometer ultraviolet light for 10 seconds, then maintain at 60 °C for 5 minutes, and then heat up to 80 °C at a rate of 3 °C / min for 10 minutes. By a special inactivation method, the cell wall polysaccharides and antibacterial peptides are retained, avoiding the risk of live bacteria, and the adhesion protein activity on the cell surface of the bacteria is retained during inactivation. This treatment method significantly improves the biological utilization rate compared with traditional heat inactivation.

[0009] Further, for the low - temperature plasma treatment instrument, the treatment power is 50 W and the treatment time is 30 s. The low - temperature plasma treatment can increase the surface roughness of the non - woven fabric.

[0010] Further, the auxiliary agent is citric acid or malic acid. By adding the auxiliary agent, the pH value is adjusted to weakly acidic, releasing prebiotics, with better effects.

[0011] Further, the cranberry has a particle size of 1 to 30 microns.

[0012] Further, the inactivated probiotic has a particle size of 1 to 30 microns.

[0013] Further, the non-woven fabric has a grammage of 20 to 50 g / m² and a porosity of 60 to 80%.

[0014] Further, the non-woven fabric chip is applied to feminine hygiene products, wound dressings, the surface layer of baby diapers or the lining layer of masks.

[0015] Meanwhile, the present invention also provides a preparation method of the non-woven fabric chip containing cranberries and inactivated probiotics, comprising the following steps: S1. Add 2 parts of carboxymethyl chitosan to deionized water with a mass 10 times that of it, heat up to 50 - 55 °C, stir for 15 minutes to dissolve, then slowly add 0.5 part of chitosan by mass, and stir rapidly for 30 minutes to dissolve. Chitosan has a low solubility in water and is difficult to dissolve in water. First, dissolve carboxymethyl chitosan, and the resulting solution increases the solubility of chitosan, enabling both components to dissolve and co-dissolve with each other. S2. Add 1 part of inactivated probiotics, 2 parts of cranberry powder and 1 part of auxiliary agent to the mixture obtained in step S1 in sequence, and stir evenly. S3. Slowly stir the mixture obtained in step S3 at a stirring speed of 10 revolutions per minute, and cool it down to 20 °C at a cooling rate of 1 °C every 15 minutes and keep it for 30 minutes, so that chitosan and carboxymethyl chitosan precipitate as a composite functional layer to coat the surface of inactivated probiotics and cranberry powder to form microspheres, obtaining a solid-liquid mixture. The obtained microspheres include cranberry - composite functional layer microspheres, inactivated probiotic - composite functional layer microspheres and cranberry - inactivated probiotic - composite functional layer microspheres. The polyphenol hydroxyl groups in cranberries form a hydrogen bond network with the amino groups of chitosan, improving the mechanical strength of the fiber and enhancing the strength of the microspheres. S4. Place the non-woven fabric in the solid-liquid mixture obtained in step S3, simultaneously introduce nitrogen for mixing, and cool it down to 10 °C at a rate of 1 °C every 15 minutes and keep it for 1 - 2 hours. During this process, the composite functional layer will form again, the composite functional layer of the original microspheres will thicken, and for the microspheres attached to the non-woven fabric, a functional layer will be formed between the microspheres and the non-woven fabric, increasing the adhesion between the microspheres and the non-woven fabric and enhancing the bonding force between the microspheres and the non-woven fabric. S5. Take out the non-woven fabric obtained in step S4, process it by the impregnation-rolling process, then dry it, and cut it into chip units to obtain the non-woven fabric chip containing cranberries and inactivated probiotics.

[0016] The method for preparing the non-woven fabric chip containing cranberry and inactivated probiotics of the present invention is simple and easy to apply. The prepared chip has good antibacterial effect and is applied to feminine hygiene products, wound dressings, the surface layer of baby diapers or the lining layer of masks. Detailed implementation manners

[0017] The technical solutions in the embodiments of the present invention will be clearly and completely described below in conjunction with the embodiments of the present invention. Obviously, the described embodiments are only a part of the embodiments of the present invention, rather than all the embodiments. All other embodiments obtained by those of ordinary skill in the art based on the embodiments of the present invention without creative efforts shall fall within the protection scope of the present invention. Embodiment

[0018] A non-woven fabric chip containing cranberry and inactivated probiotics, comprising a pretreated non-woven fabric, a composite functional layer formed by chitosan and carboxymethyl chitosan, inactivated Lactobacillus plantarum with a particle size of 1 to 30 microns, cranberry powder with a particle size of 1 to 30 microns, and citric acid.

[0019] The inactivation method is: inactivate with 280-nm ultraviolet light for 10 seconds, then maintain at 60 °C for 5 minutes, and then heat up to 80 °C at a rate of 3 °C / min for 10 minutes.

[0020] The non-woven fabric pretreatment method is: the non-woven fabric is pre-treated by a low-temperature plasma processor. The low-temperature plasma processor has a processing power of 50 W and a processing time of 30 s. The non-woven fabric has a gram weight of 30 g / m² and a porosity of 70%.

[0021] The preparation method of the above non-woven fabric chip containing cranberry and inactivated probiotics includes the following steps: S1. Add 2 kg of carboxymethyl chitosan to 20 kg of ionized water, heat up to 50 - 55 °C, stir for 15 minutes to dissolve, then slowly add 1 kg of chitosan, and quickly stir for 30 minutes to dissolve; S2. Add 1 kg of inactivated probiotics, 2 kg of cranberry powder, and 1 kg of citric acid to the mixture obtained in step S1 in sequence, and stir evenly; S3. Slowly stir the mixture obtained in step S3 at a stirring speed of 10 revolutions per minute, and cool it at a cooling rate of 1 °C per 15 minutes to 20 °C, and maintain for 30 minutes, so that chitosan and carboxymethyl chitosan precipitate as a composite functional layer to coat the surface of inactivated probiotics and cranberry powder to form microspheres, obtaining a solid-liquid mixture; S4. Place the non-woven fabric in the solid-liquid mixture obtained in step S3, simultaneously introduce nitrogen for mixing, and cool it to 10 °C at a rate of 1 °C per 15 minutes, and maintain for 1 - 2 hours; S5. Take out the non-woven fabric obtained in step S4, treat it by the impregnation-rolling process, then dry it, and cut it into chip units to obtain a non-woven fabric chip containing cranberries and inactivated probiotics.

[0022] The non-woven fabric chip is applied to feminine hygiene products, wound dressings, the surface layer of baby diapers or the lining layer of masks. Example

[0023] A non-woven fabric chip containing cranberries and inactivated probiotics, comprising a pretreated non-woven fabric, a composite functional layer formed by chitosan and carboxymethyl chitosan, inactivated lactobacilli with a particle size of 1 to 30 microns, cranberry powder with a particle size of 1 to 30 microns, and malic acid.

[0024] The inactivation method is: inactivate with 280-nm ultraviolet light for 10 seconds, then keep at 60 °C for 5 minutes, and then heat up to 80 °C at a rate of 3 °C / min for 10 minutes.

[0025] The non-woven fabric pretreatment method is: the non-woven fabric is pre-treated by a low-temperature plasma processor. The low-temperature plasma processor has a processing power of 50 W and a processing time of 30 s. The non-woven fabric has a gram weight of 40 g / m² and a porosity of 60%.

[0026] The preparation method of the above non-woven fabric chip containing cranberries and inactivated probiotics includes the following steps: S1. Add 2 kg of carboxymethyl chitosan to 20 kg of ionized water, heat up to 50 - 55 °C, stir for 15 minutes to dissolve, then slowly add 1 kg of chitosan, and stir rapidly for 30 minutes to dissolve; S2. Add 1 kg of inactivated probiotics, 2 kg of cranberry powder, and 1 kg of malic acid to the mixture obtained in step S1 in sequence, and stir evenly; S3. Slowly stir the mixture obtained in step S3 at a stirring speed of 10 revolutions per minute, and cool it at a cooling rate of 1 °C per 15 minutes to 20 °C, and keep it for 30 minutes, so that chitosan and carboxymethyl chitosan precipitate as a composite functional layer to coat the surface of inactivated probiotics and cranberry powder to form microspheres, obtaining a solid-liquid mixture; S4. Place the non-woven fabric in the solid-liquid mixture obtained in step S3, simultaneously introduce nitrogen for mixing, and cool it to 10 °C at a rate of 1 °C per 15 minutes, and keep it for 1 - 2 hours; S5. Take out the non-woven fabric obtained in step S4, treat it by the impregnation-rolling process, then dry it, and cut it into chip units to obtain a non-woven fabric chip containing cranberries and inactivated probiotics.

[0027] The non-woven fabric chip is applied to feminine hygiene products, wound dressings, the surface layer of baby diapers or the lining layer of masks. Example

[0028] A non-woven fabric chip containing cranberries and inactivated probiotics, comprising a pretreated non-woven fabric, a composite functional layer formed by chitosan and carboxymethyl chitosan, inactivated Lactobacillus with a particle size of 1 to 30 microns, cranberry powder with a particle size of 1 to 30 microns, and citric acid.

[0029] The inactivation method is: inactivate with 280-nm ultraviolet light for 10 seconds, then maintain at 60 °C for 5 minutes, and then heat up to 80 °C at a rate of 3 °C / min for 10 minutes.

[0030] The non-woven fabric pretreatment method is: the non-woven fabric is pre-treated by a low-temperature plasma treatment instrument. The low-temperature plasma treatment instrument has a treatment power of 50 W and a treatment time of 30 s. The non-woven fabric has a grammage of 25 g / m² and a porosity of 78%.

[0031] The preparation method of the above non-woven fabric chip containing cranberries and inactivated probiotics includes the following steps: S1. Add 2 kg of carboxymethyl chitosan to 20 kg of ionized water, heat up to 50 - 55 °C, stir for 15 minutes to dissolve, then slowly add 1 kg of chitosan, and stir rapidly for 30 minutes to dissolve; S2. Add 1 kg of inactivated probiotics, 2 kg of cranberry powder, and 1 kg of citric acid to the mixture obtained in step S1 in sequence, and stir evenly; S3. Slowly stir the mixture obtained in step S3 at a stirring speed of 10 revolutions per minute, and cool it at a cooling rate of 1 °C every 15 minutes to 20 °C, and maintain for 30 minutes, so that chitosan and carboxymethyl chitosan precipitate as a composite functional layer to coat the surface of inactivated probiotics and cranberry powder to form microspheres, obtaining a solid-liquid mixture; S4. Place the non-woven fabric in the solid-liquid mixture obtained in step S3, simultaneously introduce nitrogen for mixing, and cool it to 10 °C at a rate of 1 °C every 15 minutes, and maintain for 1 - 2 hours; S5. Take out the non-woven fabric obtained in step S4, treat it by the impregnation-roll pressing process, then dry it, and cut it into chip units to obtain the non-woven fabric chip containing cranberries and inactivated probiotics.

[0032] The non-woven fabric chip is applied to feminine hygiene products, wound dressings, the surface layer of baby diapers, or the lining of masks.

[0033] Comparative Example 1 Remove the cranberry powder component in Example 1, and the others are the same as in Example 2, which will not be elaborated.

[0034] Comparative Example 2 Remove the inactivated probiotic component in Example 1, and the others are the same as in Example 2, which will not be elaborated.

[0035] Comparative Example 3 In step S1 of Example 1, change the feeding order to add chitosan first and then carboxymethyl chitosan. The rest is the same as in Example 2 and will not be elaborated here.

[0036] Comparative Example 4 Commercially available silver ion non-woven fabric chips.

[0037] Perform performance tests on the chips prepared in the examples and comparative examples. The test results are shown in Table 1.

[0038] Table 1

[0039] The test data of Examples 1 to 3 show that the non-woven fabric chips containing cranberries and inactivated probiotics prepared by the present invention have good antibacterial effects and strong stimulation resistance, and the technical effects are remarkable. The data of Comparative Examples 1 to 2 show that only when cranberries and inactivated probiotics are added together and play a synergistic effect can better technical effects be achieved. The data of Comparative Example 3 show that changing the feeding order of the key materials makes it difficult to form a composite functional layer, and it is difficult for the microspheres to bind to the non-woven fabric, resulting in a significant reduction in technical effects and a significant decrease in the antibacterial rate. The data of Comparative Example 4 show that the performance of the commercially available product is lower than that of the product of the present invention, and the present invention has stronger environmental protection performance and wider applications.

[0040] Although the embodiments of the present invention have been shown and described, for those of ordinary skill in the art, it can be understood that various changes, modifications, substitutions, and variations can be made to these embodiments without departing from the principles and spirit of the present invention. The scope of the present invention is defined by the appended claims and their equivalents.

Claims

1. A non-woven fabric chip containing cranberry and inactivated probiotics, characterized in that: It includes non-woven fabric, composite functional layer, inactivated probiotics, cranberry powder and additives.

2. The non-woven fabric chip containing cranberry and inactivated probiotics according to claim 1, characterized in that: The composite functional layer is chitosan and carboxymethyl chitosan, and the mass ratio of chitosan to carboxymethyl chitosan is 1:

2.

3. The non-woven fabric chip containing cranberry and inactivated probiotics according to claim 1, characterized in that: The inactivated probiotics are Lactobacillus plantarum or Lactobacillus, and the inactivation method is: inactivation by 280 nanometer ultraviolet rays for 10 seconds, then maintaining at 60° C. for 5 minutes, and then heating to 80° C. at 3° C. / min for 10 minutes.

4. The non-woven fabric chip containing cranberry and inactivated probiotics according to claim 1, characterized in that: The non-woven fabric is pre-treated with a low-temperature plasma treatment apparatus, wherein the low-temperature plasma treatment apparatus has a treatment power of 50 W and a treatment time of 30 s. The low-temperature plasma treatment can increase the surface roughness of the non-woven fabric.

5. The non-woven fabric chip containing cranberry and inactivated probiotics according to claim 1, characterized in that: The auxiliary agent is citric acid or malic acid.

6. The non-woven fabric chip containing cranberry and inactivated probiotics according to claim 1, characterized in that: The cranberry particle size is 1 to 30 microns.

7. The non-woven fabric chip containing cranberry and inactivated probiotics according to claim 1, characterized in that: The particle size of the inactivated probiotics is 1 to 30 microns.

8. The non-woven fabric chip containing cranberry and inactivated probiotics according to claim 1, characterized in that: The non-woven fabric has a gram weight of 20-50 g / m² and a porosity of 60-80%.

9. The non-woven fabric chip containing cranberry and inactivated probiotics according to any one of claims 1 to 8, characterized in that: The nonwoven fabric chip is applied to female hygiene products, wound dressings, baby diaper surfaces or mask linings.

10. A method for preparing a non-woven fabric chip containing cranberry and inactivated probiotics according to any one of claims 1 to 8, characterized in that: The following steps are involved: S1. Add 2 parts of carboxymethyl chitosan to 10 times its mass of deionized water, heat to 50-55°C, stir for 15 minutes to dissolve, then slowly add 0.5 times its mass of chitosan, stir rapidly for 30 minutes to dissolve; S2. Add 1 part of inactivated probiotics, 2 parts of cranberry powder and 1 part of additive to the mixture obtained in step S1 and stir evenly; S3. The mixed solution obtained in step S3 is slowly stirred at a stirring speed of 10 rpm, and cooled to 20° C. at a cooling rate of 1° C. / 15 minutes, and maintained for 30 minutes, so that chitosan and carboxymethyl chitosan are precipitated as a composite functional layer and coated on the surface of the inactivated probiotics and cranberry powder to form microspheres to obtain a solid-liquid mixture; S4. The nonwoven fabric is placed in the solid-liquid mixture obtained in step S3, nitrogen is introduced into the mixture, and the temperature is lowered to 10°C at 1°C / 15 minutes and maintained for 1 to 2 hours; S5. Take out the non-woven fabric obtained in step S4, treat it by dipping-rolling process, then dry it, and cut it into chip units to obtain non-woven fabric chips containing cranberry and inactivated probiotics.

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