Photometric device, sample analyzer and sample detection method

Abstract

The invention provides a photometric device, a sample analyzer and a sample detection method. The sample analyzer comprises a loading device, a reaction device; a detection device; the controller obtains the photon counting result of each detection assembly and obtains a target counting result according to the photon counting results of the at least two detection assemblies, the linear ranges of the at least two detection assemblies are at least partially not overlapped, the photoelectric detectors of the at least two detection assemblies receive optical signals emitted by the reaction liquid in the same sample container, and the target counting result is obtained according to the optical signals. The photon counting result obtained by final detection can be prevented from being influenced by the pulse accumulation effect, so that the upper limit of the linear photometric range of the chemical reaction is improved, and the detection accuracy and the detection efficiency of a relatively strong light section of the chemical reaction are improved.

Description

Technical Field

[0001] This application relates to the technical field of medical devices, and particularly to a photometric device, a sample analyzer, and a sample detection method. Background Art

[0002] In biochemical experiments and medical clinical diagnoses, a sample analyzer is usually used to add various reagents to a sample (such as body fluids like blood and urine) to cause a chemical reaction, and then to detect and analyze the component ratio in the sample. Currently, a photometer or a photometric device with a photomultiplier tube (PMT) is usually used to detect the weak light generated by a sample, such as a blood sample, after a chemiluminescence reaction, or the fluorescence emitted by a fluorescent dye.

[0003] The basic principle of a photomultiplier tube is that a single photon outputs a beam of electrons through the photomultiplier tube, corresponding to an electrical pulse signal. However, since the electrical pulse signals caused by photons entering the photomultiplier tube are randomly distributed, as the intensity of the light signal to be measured increases, the electrical pulse signals caused by photons entering the photomultiplier tube become more and more, resulting in a pulse pile-up phenomenon where two or more electrical pulses are connected together. At this time, the existing photon counting method of the photometric device still identifies multiple piled-up electrical pulses as one electrical pulse, resulting in a low counting result in the high light intensity section and an insufficient upper limit of the linear range of the photometric device. Summary of the Invention

[0004] The following is an overview of the subject matter described in detail in this application. This overview is not intended to limit the scope of protection of the claims.

[0005] Embodiments of this application provide a photometric device, a sample analyzer, and a sample detection method, which can increase the upper limit of the linear photometric range of a chemical reaction, thereby improving the detection accuracy in the stronger light section of the chemical reaction.

[0006] In a first aspect, an embodiment of this application provides a sample analyzer, including:

[0007] A loading device for loading a sample to be measured and a reagent into a sample container;

[0008] A reaction device for providing a reaction site for the sample container containing the sample to be measured and the reagent, so that the sample to be measured and the reagent in the sample container form a reaction solution;

[0009] Detection device, comprising a carrying device and at least two detection components. At least one placement position for placing the sample container is provided in the carrying device. The detection component includes a photoelectric detector and a detection circuit. The photoelectric detector is configured to receive the optical signal emitted by the reaction solution in the sample container at the placement position and convert the optical signal into a corresponding electrical signal. The detection circuit obtains a photon counting result based on the electrical signal. Among them, the linear ranges of at least two of the detection components at least partially do not overlap, and the photoelectric detectors of at least two of the detection components receive the optical signal emitted by the reaction solution in the same sample container;

[0010] A controller, configured to obtain the photon counting results of each of the detection components and obtain a target counting result based on the photon counting results of at least two of the detection components.

[0011] In one embodiment, an attenuation component is provided on the light incident side of the photoelectric detector of at least two of the detection components, and the attenuation coefficients of the attenuation components provided for at least two of the detection components are different; or, an attenuation component is provided on the light incident side of the photoelectric detector of at least one of the detection components and no attenuation component is provided on the light incident side of the photoelectric detector of at least one of the detection components. The detection circuit in the detection component provided with the attenuation component obtains the photon counting result of the photoelectric detector based on the attenuation coefficient of the attenuation component and the electrical signal obtained from the photoelectric detector.

[0012] In one embodiment, the attenuation component includes at least one of the following:

[0013] Attenuation sheet;

[0014] Filter sheet with an attenuation coefficient greater than 1;

[0015] The attenuation component composed of a combination of an attenuation sheet and a filter sheet.

[0016] In one embodiment, a scheduling component is further included. The carrying device includes at least two of the placement positions. The scheduling component schedules the sample container between at least two of the placement positions, and at least two of the detection components are located at different placement positions.

[0017] In one embodiment, the scheduling component includes at least one of the following:

[0018] Detection disk, the placement positions are arranged on the detection disk, and at least two of the detection components are arranged at intervals along the circumference of the detection disk;

[0019] Manipulator, used to grab the sample container and move it between each of the placement positions.

[0020] In one embodiment, at least two of the detection components are arranged at intervals along the circumference of the same placement position.

[0021] In one embodiment, the controller is configured to compare at least two photon counting results with a preset threshold condition, and obtain the target counting result according to the comparison result and the at least two photon counting results.

[0022] In one embodiment, at least two of the detection components include a first detection component and a second detection component, and the light intensity of the incident light of the photodetector of the first detection component is less than the light intensity of the incident light of the photodetector of the second detection component; the controller is configured to compare the photon counting result obtained by the first detection component or the second detection component with the preset threshold condition, and obtain the target counting result according to the comparison result and the photon counting result obtained by the first detection component or the second detection component.

[0023] In one embodiment, obtaining the target counting result according to the comparison result and the photon counting result obtained by the first detection component or the second detection component includes at least one of the following:

[0024] When the photon counting result of the first detection component or the second detection component is less than a first preset threshold, the photon counting result detected by the second detection component is used as the target counting result;

[0025] When the photon counting result of the first detection component or the second detection component is greater than or equal to the first preset threshold and less than the second preset threshold, the photon counting results of the first detection component and the second detection component are fused to obtain the target counting result;

[0026] When the photon counting result of the first detection component or the second detection component is greater than or equal to the second preset threshold, the photon counting result detected by the first detection component is used as the target counting result.

[0027] In one embodiment, an attenuation component is provided on the light incident side of the photodetectors of the first detection component and the second detection component. The attenuation coefficient of the attenuation component of the first detection component is greater than that of the attenuation component of the second detection component. The detection circuits in the first detection component and the second detection component obtain the photon counting results of the first detection component and the second detection component based on the electrical signals converted by the photodetectors and the attenuation coefficients. Alternatively, the first detection component is not provided with the attenuation component, the second detection component is provided with the attenuation component, and the detection circuit in the second detection component obtains the photon counting result of the second detection component based on the electrical signal converted by the photodetector and the attenuation coefficient of the attenuation component.

[0028] In one embodiment, the controller is configured to select one of at least two photon counting results as the target counting result according to the comparison result, or perform a fusion calculation based on the comparison result and at least two photon counting results to obtain the target counting result.

[0029] In one embodiment, the controller is configured to perform a fusion calculation based on each of the photon counting results to obtain the target counting result. The fusion calculation is to perform a weighted summation calculation on each of the photon counting results to obtain the target counting result, where each of the weighting coefficients in the weighted summation calculation is a preset value or is set according to at least one of at least two of the photon counting results.

[0030] In a second aspect, an embodiment of the present application provides a sample analyzer, including:

[0031] A loading device for loading a sample to be tested and a reagent into a sample container;

[0032] A reaction device for providing a place for reaction and incubation for the sample container containing the sample to be tested and the reagent, so that the sample to be tested and the reagent in the sample container form a reaction solution;

[0033] A first detection component, the first detection component includes a first photodetector and a first detection circuit. The first photodetector is configured to receive the optical signal emitted by the reaction solution in the sample container and convert the optical signal into a first electrical signal. The first detection circuit obtains a first photon counting result based on the first electrical signal;

[0034] A second detection component, the second detection component includes a second photodetector and a second detection circuit, the second photodetector is configured to receive the optical signal emitted by the reaction solution in the sample container and convert the optical signal into a second electrical signal, and the second detection circuit obtains a second photon counting result according to the second electrical signal; wherein, the first photodetector and the second photodetector receive the optical signal emitted by the reaction solution in the same sample container, and the incident light intensity of the first photodetector is less than the incident light intensity of the second photodetector;

[0035] A controller, configured to obtain the first photon counting result and the second photon counting result, and obtain a target counting result according to the first photon counting result and the second photon counting result.

[0036] In one embodiment, the second detection component further includes an attenuation component disposed on the light incident side of the second photodetector, and no attenuation component is disposed on the light incident side of the first photodetector; the second detection circuit obtains the second photon counting result according to the attenuation coefficient of the attenuation component and the electrical signal obtained from the second photodetector;

[0037] Alternatively, the controller is configured to obtain the target counting result according to the first photon counting result, the second photon counting result, and the attenuation coefficient of the attenuation component, and the second photon counting result is obtained according to the electrical signal obtained from the second photodetector.

[0038] In one embodiment, attenuation components are disposed on the light incident sides of both the first photodetector and the second photodetector, wherein, a first attenuation coefficient of the attenuation component of the first photodetector is less than a second attenuation coefficient of the attenuation component of the second photodetector; the first photon counting result is obtained according to the first attenuation coefficient and the electrical signal obtained from the first photodetector.

[0039] In a third aspect, an embodiment of the present application provides a photometric device, including

[0040] At least two detection components, the detection component includes a photodetector and a detection circuit, the photodetector is configured to receive the optical signal emitted by the reaction solution in the sample container and convert the optical signal into a corresponding electrical signal, and the detection circuit obtains a photon counting result according to the electrical signal; wherein, at least part of the linear ranges of at least two detection components do not overlap, the reaction solution is formed by a sample to be measured and a reagent, and the photodetectors of at least two detection components receive the optical signal emitted by the reaction solution in the same sample container;

[0041] A controller, configured to obtain the photon counting results of each of the detection components and obtain a target counting result based on the multiple photon counting results.

[0042] In a fourth aspect, an embodiment of the present application provides a sample detection method, including:

[0043] Loading a sample to be tested and a reagent into a sample container to form a reaction solution; and

[0044] Detecting the reaction solution in the sample container in the following manner:

[0045] Using multiple detection components to receive optical signals emitted by the reaction solution in the sample container, and respectively converting the optical signals into electrical signals corresponding to each of the detection components, and obtaining photon counting results based on the electrical signals, wherein different detection components obtain different photon counting results, and at least two of the photodetectors of the detection components receive optical signals emitted by the reaction solution in the same sample container;

[0046] Obtaining the photon counting results of each of the detection components and obtaining a target counting result based on the multiple photon counting results

[0047] Wherein, at least part of the linear ranges of at least two of the detection components do not overlap, or the incident light intensities of the photodetectors of at least two of the detection components are different.

[0048] On the other hand, an embodiment of the present application further provides an electronic device, including a memory and a processor, the memory stores a computer program, and when the processor executes the computer program, the sample detection method of any one of the above embodiments is implemented.

[0049] On the other hand, an embodiment of the present application further provides a computer-readable storage medium, the storage medium stores a computer program, and the computer program is executed by a processor to implement the sample detection method of any one of the above embodiments.

[0050] On the other hand, an embodiment of the present application further provides a computer program product, the computer program product includes a computer program, the computer program is stored in a computer-readable storage medium. The processor of the computer device reads the computer program from the computer-readable storage medium, and the processor executes the computer program, so that the computer device executes the sample detection method of any one of the above embodiments.

[0051] The photometric device, sample analyzer and sample detection method provided by the embodiments of the present application have at least the following beneficial effects:

[0052] The photon count results are obtained by sequentially detecting the photons of the reaction solution in the same sample container through at least two detection components in the detection device. Then, the controller obtains the photon count results of each detection component, and obtains the target count result of the reaction solution based on the photon count results of at least two detection components. Among them, the linear ranges of at least two detection components do not overlap at least partially, which can avoid the influence of pulse pile-up phenomenon on photon counting when using a single detection component to detect the high light intensity section, thereby increasing the upper limit of the linear photometric range of the chemical reaction and improving the detection accuracy of the strong light section of the chemical reaction. And by receiving the optical signal emitted by the reaction solution in the same sample container through the photodetectors of at least two detection components, two photon count results can be obtained in a short time, which is conducive to improving the chemiluminescence detection efficiency of the sample analyzer. In addition, the photodetector in the detection component is used to convert the optical signal into an electrical signal and send it to the detection circuit for processing to obtain the photon count result of the reaction solution in the same sample container, and the upper limit of the linear photometric range of the sample analyzer can be increased without designing a complex calculation circuit for photon counting processing.

[0053] Other features and advantages of the present application will be described in the subsequent specification, and, in part, will be obvious from the specification, or will be understood by implementing the present application. Description of the Drawings

[0054] The drawings are used to provide a further understanding of the technical solutions of the present application, and constitute a part of the specification. Together with the embodiments of the present application, they are used to explain the technical solutions of the present application, and do not constitute a limitation to the technical solutions of the present application.

[0055] Figure 1 It is a schematic curve diagram of a pulse signal with normal pulses;

[0056] Figure 2 It is a schematic curve diagram of a pulse signal with piled-up pulses;

[0057] Figure 3 It is a structural block diagram of a sample analyzer provided by an embodiment of the present invention;

[0058] Figure 4 It is a schematic block diagram of photometric measurement through a detection component in a detection device provided by an embodiment of the present invention;

[0059] Figure 5 It is a schematic block diagram of a detection circuit processing an electrical signal to obtain a photon count result provided by an embodiment of the present invention;

[0060] Figure 6 It is a schematic block diagram of photometric measurement after adding an attenuation component to a detection device provided by an embodiment of the present invention;

[0061] Figure 7 The photometric linearity characteristic curve graph of the detection device of the prior art and the detection device of the sample analyzer of the present invention;

[0062] Figure 8 The schematic block diagram of photometric measurement using attenuation components with multiple different attenuation coefficients provided by an embodiment of the present invention;

[0063] Figure 9 The schematic block diagram of photometric measurement using a single attenuation component provided by an embodiment of the present invention

[0064] Figure 10 The structural block diagram of the sample analyzer provided by another embodiment of the present invention;

[0065] Figure 11 The schematic block diagram of obtaining the photon counting result according to multiple different attenuation coefficients provided by an embodiment of the present invention;

[0066] Figure 12 The structural block diagram of the photometric device provided by an embodiment of the present invention;

[0067] Figure 13 The schematic structural diagram of the chemiluminescence immunoassay analyzer constructed according to the sample analyzer provided by an embodiment of the present invention;

[0068] Figure 14 The schematic flowchart of the sample detection method provided by an embodiment of the present invention. Detailed implementation manners

[0069] In order to make the objectives, technical solutions and advantages of the present application clearer, the present application will be further described in detail below with reference to the accompanying drawings and embodiments. It should be understood that the specific embodiments described herein are only used to explain the present application and are not used to limit the present application.

[0070] In the following description, reference is made to "some embodiments", which describe a subset of all possible embodiments. However, it can be understood that "some embodiments" can be the same subset or different subsets of all possible embodiments, and can be combined with each other without conflict.

[0071] Unless otherwise defined, all technical and scientific terms used herein have the same meaning as commonly understood by those skilled in the technical field to which this application belongs. The terms used herein are only for the purpose of describing the embodiments of this application and are not intended to limit this application.

[0072] In the description of this application and the above-mentioned drawings, terms such as "first", "second", "third", "fourth", etc. (if any) are used to distinguish similar objects and do not necessarily describe a specific order or sequence. It should be understood that the data used in this way can be interchanged under appropriate circumstances so as to describe the embodiments of this application. For example, it can be implemented in an order other than those illustrated or described herein. In addition, the terms "comprising" and "having" and any variations thereof are intended to cover non-exclusive inclusion. For example, a process, method, system, product, or device comprising a series of steps or units does not necessarily have to be limited to those steps or units clearly listed, but may include other steps or units not clearly listed or inherent to these processes, methods, products, or devices.

[0073] It should be understood that in this application, "at least one (item)" means one or more, and "a plurality" means two or more. "And / or" is used to describe the association relationship of associated objects and indicates that three relationships can exist. For example, "A and / or B" can mean: only A exists, only B exists, and both A and B exist simultaneously. Here, A and B can be singular or plural. The character " / " generally indicates that the associated objects before and after are in an "or" relationship. "At least one (one) of the following" or similar expressions refer to any combination of these items, including any combination of single items (ones) or plural items (ones). For example, at least one (one) of a, b, or c can mean: a, b, c, "a and b", "a and c", "b and c", or "a and b and c", where a, b, c can be single or multiple.

[0074] It should be understood that in the description of the embodiments of this application, the meaning of "a plurality (or multiple items)" is more than two. Understandings such as greater than, less than, exceeding, etc. do not include the present number, and understandings such as above, below, within, etc. include the present number.

[0075] As mentioned in the background art, the basic principle of a photomultiplier tube is that a single photon outputs a beam of electrons through the photomultiplier tube, corresponding to an electrical pulse signal. However, since the electrical pulse signals caused by photons entering the photomultiplier tube are randomly distributed, as the intensity of the light signal to be measured increases, the electrical pulse signals caused by photons entering the photomultiplier tube become more and more, resulting in a pulse pile-up phenomenon where two or more electrical pulses are connected together. Refer to Figure 1 and Figure 2 shown, Figure 1 shows the electrical pulse signal under normal conditions, while Figure 2 shows the electrical pulse signal under the pulse pile-up condition. Among them, when the peak value of the identified pulse is greater than the threshold, it is considered that a photon is identified. The photon counting method of the existing photometric device can accurately detect the number of photons in the case of Figure 1 , but in the case of Figure 2In this case, multiple stacked electrical pulses will be recognized as one electrical pulse, resulting in a lower count result for the higher light intensity segment.

[0076] Therefore, in view of the technical problem that the existing photon counting method cannot accurately detect the light signal intensity in a strong light segment due to the pulse pile-up phenomenon, the present application proposes a technical solution that can expand the linear photometric range. In this technical solution, the light signal emitted after the chemical reaction of the receiving reaction solution is received and converted into a corresponding electrical signal, then the photon counting result is obtained according to the electrical signal, and the target counting result of the reaction solution is calculated based on at least two photon counting results. Thereby, the influence of the pulse pile-up effect on the photon counting result is avoided, the linear photometric range of the chemical reaction is improved, and the detection accuracy and detection efficiency of the stronger light segment emitted by the reaction solution are improved.

[0077] Referring to Figure 3 As shown, an embodiment of the first aspect of the present application provides a sample analyzer 100, which includes a loading device 110, a reaction device 120, a detection device 130, and a controller 140. The chemiluminescence reaction of the sample analyzer 100 is set such that the reaction solution can emit a weak light signal not greater than 200 million photons per second after the chemiluminescence reaction.

[0078] The loading device 110 is used to load the test sample and the reagent into the sample container.

[0079] The reaction device 120 is used to provide a reaction site for the sample container containing the test sample and the reagent, so that the test sample and the reagent in the sample container form a reaction solution.

[0080] The detection device 130 includes a carrier device 131 and at least two detection components. At least one placement position for placing the sample container is provided in the carrier device 131. The detection component includes a photodetector and a detection circuit. The photodetector is configured to receive the light signal emitted by the reaction solution in the sample container at the placement position and convert the light signal into a corresponding electrical signal. The detection circuit obtains the photon counting result according to the electrical signal.

[0081] The controller 140 is configured to obtain the photon counting results of each detection component and obtain the target counting result according to the photon counting results of at least two detection components.

[0082] It can be understood that the photon counting result is obtained by sequentially detecting the number of photons of the reaction solution in the same sample container by at least two detection components in the detection device 130, and then the controller 140 obtains the photon counting results of each detection component and obtains the target counting result of the reaction solution according to the photon counting results of at least two detection components, wherein the linear ranges of at least two detection components at least partially do not overlap. For example, in an embodiment of the present application, Figure 3In the detection device 130, two detection components can be provided, including a first detection component 1301 and a second detection component 1302. Among them, the linear ranges of the first detection component 1301 and the second detection component 1302 at least partially do not overlap, which can avoid the influence of pulse pile-up phenomenon on photon counting when using a single detection component to detect the high light intensity section, thereby increasing the upper limit of the linear photometric range of the chemical reaction and improving the detection accuracy of the stronger light section of the chemical reaction. And by the photodetectors of at least two detection components receiving the optical signals emitted by the reaction solution in the same sample container, two photon counting results can be obtained in a short time, which is conducive to improving the chemiluminescence detection efficiency of the sample analyzer 100. In addition, through the photodetector in the detection component, the optical signal is converted into an electrical signal and sent to the detection circuit for processing to obtain the photon counting result of the reaction solution in the same sample container, and the upper limit of the linear photometric range of the sample analyzer can be increased without designing a complex calculation circuit for photon counting processing.

[0083] Further, the loading device can load the blood sample and the reagents required for the chemiluminescence reaction into the sample container; referring to Figure 4 As shown in the figure, in an implementation manner of the embodiment of the present application, the detection device 130 includes two detection components, specifically including a first detection component 1301 and a second detection component 1302. Among them, the photodetectors in each detection component are electrically connected to the detection circuit. Specifically, the first photodetector in the first detection component 1301 is electrically connected to the first detection circuit, and the second photodetector in the second detection component 1302 is electrically connected to the second detection circuit. Furthermore, the first detection circuit and the second detection circuit are respectively connected to the controller 140 and output the first photon counting result and the second photon counting result. It can be understood that the photodetector in the embodiment of the present application can be a photomultiplier tube, and the photomultiplier tube receives the optical signal emitted after the chemiluminescence reaction of the reaction solution and converts the optical signal into a corresponding electrical signal. In one embodiment, referring to Figure 5 As shown in the figure, the detection circuit in each detection component can include a pre-amplification circuit, a level discrimination circuit, a shaping and frequency division circuit, and a counting circuit. The pre-amplification circuit is configured to receive the electrical signal output by the photomultiplier tube and amplify the electrical signal. The level discrimination circuit is configured to convert the electrical signal output by the pre-amplification circuit into a square wave signal. The shaping and frequency division circuit is configured to perform frequency division and shaping on the square wave signal. The counting circuit is configured to count the signal output by the shaping and frequency division circuit, that is, identify the number of pulses. Therefore, an accurate photon counting result can be obtained, especially improving the detection accuracy of the stronger light section emitted by the reaction solution and increasing the linear photometric range of the detection device 130. Figure 4The first detection component 1301 and the second detection component 1302 in it are only one of the embodiments. It can be understood that in addition to setting the above two detection components, namely the first detection component 1301 and the second detection component 1302, the detection device 130 can also set more detection components. For example, it can set more than three detection components, and at least part of the linear ranges of these detection components do not overlap.

[0084] In one embodiment, an attenuation component 150 is provided on the light incident side of the photodetectors of at least two detection components, and the attenuation coefficients of the attenuation components 150 provided for at least two detection components are different; or, an attenuation component 150 is provided on the light incident side of the photodetector of at least one detection component and no attenuation component 150 is provided on the light incident side of the photodetector of at least one detection component. The detection circuit in the detection component provided with the attenuation component 150 obtains the photon counting result of the photodetector according to the attenuation coefficient of the attenuation component 150 and the electrical signal obtained from the photodetector.

[0085] In the embodiment of the present application, an attenuation component 150 can be provided at least on the light incident side of the photodetectors of two detection components. When measuring the light of the reaction solution that undergoes a chemiluminescence reaction in the same sample container, the sample container containing the reaction solution is sequentially placed in front of the photodetectors with attenuation components 150 having different attenuation coefficients. The light signal emitted by the reaction solution first passes through the attenuation components 150 with different attenuation coefficients and reduces the light intensity with different attenuation coefficients. Refer to Figure 6 As shown, in one embodiment, when the detection device 130 is provided with two detection components, one of the attenuation components 150 with an attenuation coefficient of n1 can be first selected and set in front of one of the photodetectors, and the intensity of the light signal emitted by the reaction solution can be attenuated by n1 times. Another attenuation component 150 with an attenuation coefficient of n2 is set in front of the other photodetector, and the intensity of the light signal emitted by the reaction solution can be attenuated by n2 times. Then, the light signals with attenuated light intensities are processed by the pre-amplification circuit, level discrimination circuit, shaping and frequency division circuit, and counting circuit of each photodetector and the detection circuit, and finally at least two photon counting results are obtained. In one embodiment, or when the detection device 130 is provided with two detection components, an attenuation component 150 can also be only set on the light incident side of the photodetector of one detection component, and no attenuation component 150 is set on the photodetector of the other detection component. Then, the detection circuit in the detection component provided with the attenuation component 150 processes the light signal with attenuated light intensity according to the attenuation coefficient of the attenuation component 150, and at least one photon counting result is obtained. Thus, by setting the attenuation component 150, the linear light measurement range of the chemical reaction is adjusted, the pulse pile-up effect is avoided from affecting the photon counting result, and the light measurement accuracy and detection efficiency are improved. Figure 6In one embodiment of the present application, an attenuation component 150 is provided on the light incident side of the photodetectors of two detection components. It should be understood that the detection device 130 may be provided with multiple detection components, for example, two or more than three detection components may be provided, and the linear ranges of these detection components at least partially do not overlap. Further, multiple attenuation components 150 may be provided on the light incident sides of the photodetectors of multiple detection components, for example, two or more than three attenuation components 150 may be provided.

[0086] It should be noted that the light signal intensity of the chemiluminescence of the reaction solution can be attenuated by n times by using the attenuation component 150, where n is the attenuation coefficient and n is an integer greater than 1. Refer to Figure 7 As shown in the figure, where the curve S1 represents the photometric linear characteristic of the photometric device in the prior art, and the curve S2 represents the photometric linear characteristic of the photometric device of the present application. The present application can select an attenuation component 150 with any appropriate attenuation coefficient according to specific photometric requirements, and configure the controller 140 to perform compensation processing on the photon counting result according to the attenuation coefficient. For example, selecting an attenuation component 150 with an attenuation coefficient of 10, 20, or 30 can increase the upper limit of the linear photometric range of the detection device 130 by 10, 20, or 30 times or more. When the attenuation coefficient is 10, the linear photometric range is expanded from the original 0 to 30 million photons per second (i.e., 0 to 30 million photons per second) to 0 to 300 million photons per second (i.e., 0 to 300 million photons per second). Those skilled in the art should understand that the larger the attenuation coefficient of the attenuation component 150 provided on the light incident side of the photodetector in the embodiment of the present application, the larger the upper limit of the linear photometric range of the detection device 130 can be ultimately increased, thereby improving the photon counting accuracy in the strong light section.

[0087] In one embodiment, the attenuation component 150 may adopt an attenuation film, also known as a neutral density filter. By using different attenuation films, the incident light intensity of the reaction solution can be reduced according to different attenuation coefficients.

[0088] In another embodiment of the present application, the attenuation component 150 may adopt a filter film with an attenuation coefficient greater than 1. The filter film has a strong absorption or scattering effect on light, and the light intensity received by the photodetector after passing through the filter film is lower than the light intensity without passing through the filter film. The light intensity received by the photodetector can be adjusted according to the selection of different attenuation coefficient values.

[0089] It should be understood that the attenuation component 150 provided on the light incident side of the photodetector may also be composed of a combination of an attenuation film and a filter film. An attenuation component 150 with any appropriate attenuation coefficient is used to adjust the linear photometric range of the chemical reaction to obtain an accurate photon counting result.

[0090] In one embodiment, the sample analyzer 100 further includes a scheduling component. The carrying device 131 includes at least two placement positions. The scheduling component schedules the sample containers between the at least two placement positions, and at least two detection components are located at different placement positions. By adding two or more placement positions for placing sample containers to the carrying device 131, each placement position is correspondingly provided with a detection component. For example, after a detection component disposed at one of the placement positions measures the light of a sample container loaded with a luminescent reaction solution, the scheduling component is coordinated to move and place the sample container at the next placement position for light measurement, so as to sequentially measure the light of the same sample container and finally obtain at least two photon counting results, thereby improving the detection efficiency.

[0091] In one embodiment, the scheduling component includes at least one of the following:

[0092] A detection disk, the placement positions are set on the detection disk, and at least two detection components are arranged at intervals along the circumference of the detection disk;

[0093] A manipulator for grasping the sample container and moving it between the respective placement positions.

[0094] In one embodiment, a detection disk can be used as the scheduling component, and then the same sample container is moved to different light measurement positions for light measurement. At this time, at least two detection components in the detection device 130 are arranged at intervals along the circumference with the detection disk as the center, and one or more placement positions can be set on the detection disk. When the sample container to be measured is placed at the placement position, the detection disk rotates to make the sample container correspondingly rotate to the light measurement position provided with the detection component for light measurement, and then rotates to the next light measurement position for secondary light measurement. In another embodiment, a manipulator can also be used as the scheduling component. The manipulator is used to grasp the same sample container and schedule and move the sample container between the respective placement positions provided with detection components. Further, the manipulator is configured to be able to rotate horizontally and perform two-dimensional up and down movements.

[0095] In one embodiment, at least two detection components in the detection device 130 are arranged at intervals along the circumference of the same placement position.

[0096] In one embodiment, Figure 6 The controller 140 in is configured to compare at least two photon counting results with a preset threshold condition, and obtain a target counting result according to the comparison result and the at least two photon counting results. It should be understood that in the embodiments of the present application, the preset threshold condition can be set according to actual light measurement requirements and stored in the controller 140 in advance, so that more accurate photon counting results can be obtained.

[0097] Further, the controller 140 may be configured to select one of at least two photon counting results as the target counting result according to the comparison result, or perform a fusion calculation based on the comparison result and the at least two photon counting results to obtain the target counting result.

[0098] In another embodiment, the controller 140 may also be configured to perform a fusion calculation based on each photon counting result to obtain the target counting result. The fusion calculation is to perform a weighted summation calculation on each photon counting result to obtain the target counting result, where each weighting coefficient in the weighted summation calculation is a preset value or is set according to at least one of the at least two photon counting results.

[0099] Specifically, at least two detection components of the detection device include a first detection component 1301 and a second detection component 1302. The light intensity of the incident light of the photodetector of the first detection component 1301 is less than the light intensity of the incident light of the photodetector of the second detection component 1302; the controller 140 is configured to compare the photon counting result obtained by the first detection component 1301 or the second detection component 1302 with a preset threshold condition, and obtain the target counting result according to the comparison result and the photon counting result obtained by the first detection component 1301 or the second detection component 1302.

[0100] It can be understood that by sequentially measuring the number of photons of the reaction solution in the same sample container by the first detection component 1301 and the second detection component 1302 of the detection device, at least one attenuation component 150 is provided in the first detection component 1301 and the second detection component 1302 of this embodiment. For example, an attenuation component 150 is provided on the light incident side of the photodetector of the first detection component 1301, and no attenuation component 150 is provided on the light incident side of the photodetector of the second detection component 1302. When the light emission intensity of the reaction solution is relatively strong, the controller 140 is configured to compare the photon counting result of the first detection component 1301 or the photon counting result of the second detection component 1302 with a preset threshold condition. The light intensity of the incident light of the photodetector of the first detection component 1301 is less than the light intensity of the incident light of the photodetector of the second detection component 1302, and the photon counting result of the first detection component 1301 is less than the preset threshold condition. Therefore, the final target counting result is calculated according to the comparison result and the photon counting result of the first detection component 1301 through the attenuation coefficient of the provided attenuation component 150, avoiding the influence of the pulse pile-up effect on the photon counting result, and further improving the photometric accuracy and detection efficiency.

[0101] In one embodiment, obtaining the target counting result according to the comparison result and the photon counting result obtained by the first detection component 1301 or the second detection component 1302 includes at least one of the following:

[0102] When the photon counting result of the first detection component 1301 or the second detection component 1302 is less than the first preset threshold, the photon counting result obtained by detecting with the second detection component 1302 is used as the target counting result. It can be understood that when the luminescence intensity of the reaction solution is weak, no pulse pile-up effect occurs. The controller 140 can be configured to judge the photon counting result using the first preset threshold. If the photon counting results detected by the first detection component 1301 or the second detection component 1302 are not affected and the comparison results are both less than the first preset threshold, the photon counting result obtained by the second detection component 1302 can be selected as the target counting result.

[0103] When the photon counting result of the first detection component 1301 or the second detection component 1302 is greater than or equal to the first preset threshold and less than the second preset threshold, the photon counting results of the first detection component 1301 and the second detection component 1302 are fused to obtain the target counting result. It should be understood that when the luminescence intensity of the reaction solution is medium, the controller 140 can be configured to judge the photon counting result using the first preset threshold and the second preset threshold. If the photon counting result of the first detection component 1301 or the second detection component 1302 is within the range of the first preset threshold and the second preset threshold, the photon counting results detected by the first detection component and the second detection component 1302 can be fused and calculated to obtain the target counting result. Specifically, the fusion calculation is to perform a weighted summation calculation on the photon counting results obtained by the first detection component 1301 and the second detection component 1302 to obtain the target counting result, where each weighting coefficient in the weighted summation calculation is a preset value or is set according to at least one of the photon counting results of the first detection component 1301 and the second detection component 1302. For example, the functional relationship between the first preset threshold and the second preset threshold and each photon counting result can be determined by looking up a table or a curve. This functional relationship can be a weighted average function. Then, according to the result of looking up the table or the curve, at least one weighting coefficient of each photon counting result can be determined. If the photon counting result is closer to the first preset threshold or the second preset threshold, the corresponding weighting coefficient is larger. On the contrary, if the photon counting result is farther from the first preset threshold or the second preset threshold, the corresponding weighting coefficient is smaller. In this way, the finally obtained photon counting result is more linear, further improving the accuracy of the photon counting result. It can be understood that in the embodiment of the present application, the situation that triggers the controller 140 to perform the fusion function calculation can be that the photon counting result of the first detection component 1301 or the photon counting result of the second detection component 1302 is between the first preset threshold and the second preset threshold, and the sum of the weighting coefficients corresponding to each photon counting result is 1.

[0104] When the photon counting result of the first detection component 1301 or the second detection component 1302 is greater than or equal to the second preset threshold, the photon counting result obtained by detecting with the first detection component 1301 is used as the target counting result. It should be noted that when the luminescence intensity of the reaction solution is strong, an attenuation component 150 can be provided on the light incident side of the photodetector of the first detection component 1301 to reduce the influence on the photon counting result obtained by the first detection component 1301. However, the photon counting result detected by the second detection component 1302 is greater than or equal to the second threshold, resulting in a pulse pile-up effect. Therefore, the controller 140 is configured to select the photon counting result obtained by the first detection component 1301 as the target counting result.

[0105] It should be noted that the first preset threshold and the second preset threshold in the embodiments of the present application can be set according to actual photometric requirements and pre-stored in the controller 140, so that a more accurate photon counting result can be obtained.

[0106] Refer to Figure 8As shown, in one embodiment, an attenuation component 150 is provided on the light incident side of the photodetectors of both the first detection component 1301 and the second detection component 1302. The attenuation coefficient of the attenuation component 150 of the first detection component 1301 is greater than that of the attenuation component 150 of the second detection component 1302. The detection circuits in the first detection component 1301 and the second detection component 1302 obtain the photon counting results of the first detection component 1301 and the second detection component according to the electrical signals converted by the photodetectors and the attenuation coefficients. Specifically, attenuation components 150 with different attenuation coefficients are provided on the light incident side of the first photodetector of the first detection component 1301 and the light incident side of the second photodetector of the second detection component 1302. The first detection circuit in the first detection component 1301 obtains the first photon counting result according to the electrical signal converted by the first photodetector and the attenuation coefficient. The second detection circuit in the second detection component 1302 obtains the second photon counting result according to the electrical signal converted by the second photodetector and the attenuation coefficient. Among them, the attenuation component 150 with a larger attenuation coefficient makes the incident light intensity of the first photodetector less than that of the second photodetector provided with the attenuation component 150 with a smaller attenuation coefficient. Furthermore, the detection circuits in the first detection component 1301 and the second detection component calculate the photon counting results of the first detection component 1301 and the second detection component 1302 respectively according to the electrical signals converted by the photodetectors and the two attenuation coefficients. For example, the larger attenuation coefficient is N1 and the smaller attenuation coefficient is N2. When measuring the light of the reaction solution for chemiluminescence, the second detection component 1302 can be used for measurement first, and then the first detection component 1301 can be used for measurement. At this time, the photon counting result detected by the first detection component 1301 can be recorded as the first photon counting result (attenuation coefficient N1), and the photon counting result detected by the second detection component 1302 can be recorded as the second photon counting result (attenuation coefficient N2). Further, the controller 140 can be configured to compare the first photon counting result or the second photon counting result with the first preset threshold and the second preset threshold respectively. Since attenuation sheets are provided in both the first detection component 1301 and the second detection component 1302, when the light emission intensity of the reaction solution is weak, the first photon counting result or the second photon counting result is less than the first preset threshold, and the second photon counting result obtained according to the attenuation coefficient N2 can be used as the target result; when the light emission intensity of the reaction solution is medium, the first photon counting result or the second photon counting result is greater than or equal to the first preset threshold and less than the second preset threshold, and the first photon counting result and the second photon counting result can be fused and calculated to obtain the target counting result; when the light emission intensity of the reaction solution is strong, the first photon counting result or the second photon counting result is greater than or equal to the second preset threshold, and the first photon counting result obtained according to the attenuation coefficient N1 can be used as the target result.

[0107] Further, the controller 140 may be configured to perform compensation processing on the first photon counting result and the second photon counting result according to the attenuation coefficient N1 and the attenuation coefficient N2, respectively. The value n1 of the attenuation coefficient N1 and the value n2 of the attenuation coefficient N2 are both integers greater than 1, and n1 is greater than n2. For example, selecting an attenuation component 150 with an attenuation coefficient N1 of 20 can increase the upper limit of the linear photometric range of the first detection component 1301 by 20 times, that is, expand the linear photometric range from the original 0 to 30 million photons per second (i.e., 0 to 30 million photons per second) to 0 to 600 million photons per second (i.e., 0 to 600 million photons per second). Or an attenuation component 150 with an attenuation coefficient N2 of 10 can be selected, so as to increase the upper limit of the linear photometric range of the first detection component 1301 by 10 times, that is, expand the linear photometric range from the original 0 to 30 million photons per second (i.e., 0 to 30 million photons per second) to 0 to 300 million photons per second (i.e., 0 to 300 million photons per second).

[0108] In another embodiment, alternatively, the first detection component 1301 is not provided with an attenuation component 150, and the second detection component 1302 is provided with an attenuation component 150. The detection circuit in the second detection component 1302 obtains the photon counting result of the second detection component 1302 according to the electrical signal converted by the photodetector and the attenuation coefficient of the attenuation component 150.

[0109] Refer to Figure 9 As shown, in an implementation manner of the embodiment of the present application, when the luminescence intensity of the reaction solution is relatively strong, due to the action of the attenuation component 150 in the second detection component 1302, the light signal intensity received by the second photodetector in the second detection component 1302 is reduced and no pulse pile-up effect occurs. The first photon counting result of the first detection component 1301 is affected by the pulse pile-up effect. Therefore, the second photon counting result obtained by the second detection circuit in the second detection component 1302 according to the electrical signal converted by the second photodetector and the attenuation coefficient of the attenuation component 150 is used as the target counting result, thereby increasing the upper limit of the linear photometric range.

[0110] Further, Figure 9An attenuation component 150 with an attenuation coefficient of n can be selected and arranged on the light incident side of the photodetector of the second detection component 1302. For example, it is arranged on the light incident side of the second photodetector to reduce the intensity of the incident light emitted by the reaction solution, where n is an integer greater than 1. At this time, the target count result is equal to n times the photon count result of the second detection component 1302. Specifically, the controller 140 is correspondingly configured to perform compensation processing on the photon count result according to the attenuation coefficient. For example, selecting an attenuation component 150 with an attenuation coefficient of 10 can increase the upper limit of the linear photometric range of the detection device by 10 times, that is, the target count result is equal to 10 times the photon count result of the second detection component 1302, and the linear photometric range can be extended from the original 0 to 30 million photons per second (i.e., 0 to 30 million photons per second) to 0 to 300 million photons per second (i.e., 0 to 300 million photons per second). It can be understood that in the embodiment of the present application, the intensity of the optical signal received by the photodetector provided with the attenuation component 150 with an attenuation coefficient of n is 1 / n of the intensity of the optical signal received by the photodetector without the attenuation component 150.

[0111] Referring to Figure 10 As shown, an embodiment of the second aspect of the present application provides a sample analyzer 200, including a loading device 110, a reaction device 120, a first detection component 1301, a second detection component 1302, and a controller 140. Among them, the loading device 110 is used to load a sample to be tested and a reagent into a sample container; the reaction device 120 is used to provide a place for reaction and incubation for the sample container loaded with the sample to be tested and the reagent, so that the sample to be tested and the reagent in the sample container form a reaction solution; the first detection component 1301 includes a first photodetector and a first detection circuit. The first photodetector is configured to receive the optical signal emitted by the reaction solution in the sample container and convert the optical signal into a first electrical signal. The first detection circuit obtains a first photon count result according to the first electrical signal; the second detection component 1302 includes a second photodetector and a second detection circuit. The second photodetector is configured to receive the optical signal emitted by the reaction solution in the sample container and convert the optical signal into a second electrical signal. The second detection circuit obtains a second photon count result according to the second electrical signal; the controller 140 is configured to obtain the first photon count result and the second photon count result, and obtain a target count result according to the first photon count result and the second photon count result.

[0112] It can be understood that the first photodetector and the second photodetector receive the optical signals emitted by the reaction solution in the same sample container, and the incident light intensity of the first photodetector is less than that of the second photodetector. This can avoid the influence of pulse pile-up phenomenon on photon counting when using a single detection component to detect the high light intensity section, which is beneficial for the controller 140 to further process the first photon counting result and the second photon counting result, and then obtain an accurate target counting result, thereby improving the upper limit of the linear photometric range of the sample analyzer 200.

[0113] Since the incident light intensities of the first photodetector and the second photodetector are different, this makes the linear ranges of the first detection component 1301 and the second detection component 1302 at least partially non-overlapping. Further, the first photodetector and the second photodetector specifically include photomultiplier tubes, which receive the optical signals emitted by the reaction solution after chemiluminescence reaction and convert the optical signals into corresponding electrical signals. Further, both the first detection circuit and the second detection circuit include a pre-amplification circuit, a level discrimination circuit, a shaping and frequency division circuit, and a counting circuit. Specifically, the pre-amplification circuit is configured to receive the electrical signal output by the photomultiplier tube and amplify the electrical signal, the level discrimination circuit is configured to convert the electrical signal output by the pre-amplification circuit into a square wave signal, the shaping and frequency division circuit is configured to perform frequency division and shaping on the square wave signal, and the counting circuit is configured to count the signal output by the shaping and frequency division circuit, that is, identify the number of pulses. Therefore, accurate first photon counting result and second photon counting result can be obtained, especially improving the detection accuracy of the first detection component 1301 and the second detection component 1302 for the stronger light section emitted by the reaction solution.

[0114] Further, the linear photometric ranges of the first detection component 1301 and the second detection component 1302 at least partially do not overlap. By using the first detection component 1301 and the second detection component 1302 in combination to replace a single detection component to achieve the detection of the number of photons with a higher light intensity emitted by the reaction solution, the performance requirements for a single detection component can be reduced, thereby improving the detection efficiency. In addition, the upper limit of the linear photometric range of the sample analyzer 100 can be improved without designing a complex calculation circuit for photon counting processing.

[0115] Refer to Figure 9As shown, in one embodiment, the second detection component 1302 further includes an attenuation component 150 disposed on the light incident side of the second photodetector, and the attenuation component 150 is not disposed on the light incident side of the first photodetector; the second detection circuit obtains a second photon counting result according to the attenuation coefficient of the attenuation component 150 and the electrical signal obtained from the second photodetector. It should be understood that when the luminescence intensity of the reaction solution is relatively strong, due to the action of the attenuation component 150, the light signal intensity received by the photodetector of the second detection component 1302, such as the second photodetector, is reduced, and no pulse pile-up effect occurs. However, the photon counting result of the first detection component 1301 will be affected by the pulse pile-up effect. Therefore, the second detection circuit in the second detection component 1302 obtains a second photon counting result according to the attenuation coefficient of the attenuation component 150 and the electrical signal obtained from the second photodetector. Further, the controller 140 is configured to perform a fusion calculation according to the second photon counting result and the attenuation coefficient of the disposed attenuation component 150 to obtain a final target counting result, which can avoid the influence of the pulse pile-up effect, thereby increasing the upper limit of the linear photometric range and further improving the photometric accuracy. In another embodiment, alternatively, the controller 140 may also be configured to obtain a target counting result according to the first photon counting result, the second photon counting result, and the attenuation coefficient of the attenuation component 150, and the second photon counting result is obtained according to the electrical signal obtained from the second photodetector. For the specific details and beneficial effects of this embodiment, reference may be made to the description of the foregoing embodiments, which will not be elaborated herein.

[0116] In one embodiment, referring to Figure 8 and Figure 11As shown, attenuation components 150 are provided on the light incident sides of the first photodetector and the second photodetector. Among them, the first attenuation coefficient of the attenuation component 150 of the first photodetector is less than the second attenuation coefficient of the attenuation component 150 of the second photodetector; the first photon counting result is obtained based on the first attenuation coefficient and the electrical signal obtained by the first photodetector. When measuring the light of the reaction solution undergoing chemiluminescence reaction in the same sample container, the sample container containing the reaction solution is successively placed in front of the photodetectors provided with attenuation components 150 having different attenuation coefficients, such as the first photodetector of the first detection component 1301 and the second photodetector of the second detection component 1302. The light signal emitted by the reaction solution is successively measured by the first photodetector and the second photodetector. Specifically, the light signal generated by the chemical reaction is first received by the first photodetector provided with the attenuation component 150, and the light signal is converted into a first electrical signal according to the smaller first attenuation coefficient. Then, the first detection circuit obtains the first photon counting result based on the first electrical signal; then, the light signal emitted by the reaction solution in the same sample container is received by the second photodetector, and the light signal is converted into a second electrical signal according to the larger second attenuation coefficient. Then, the second detection circuit obtains the second photon counting result based on the second electrical signal, realizing the reduction of the light intensity of the reaction solution emission with different attenuation coefficients. Further, the controller 140 is configured to perform fusion calculation based on the first photon counting result, the second photon counting result, the first attenuation coefficient, and the second attenuation coefficient to obtain the target counting result. For example, the first photon counting result and the second photon counting result are respectively compensated according to the first attenuation coefficient and the second attenuation coefficient, and then the target counting result is calculated through a preset fusion function according to the compensation result. It can be understood that the preset fusion function can be, for example, a function for obtaining an average value or a function for obtaining a weighted average value. The corresponding weighting coefficients of the first photon counting result and the second photon counting result are the first weighting coefficient w1 and the second weighting coefficient w2 respectively. Each weighting coefficient can include a preset value pre-stored in the controller 140 or be set according to each photon counting result.

[0117] It should be understood that by providing the attenuation components 150 on the light incident sides of the first photodetector and the second photodetector, the linear light measurement range of the chemical reaction can be adjusted, the pulse pile-up effect can be avoided from affecting the photon counting result, the light measurement accuracy and the detection efficiency can be improved. By using the first detection component 1301 and the second detection component 1302 in combination to replace a single detection component to realize the detection of the number of photons with a higher light intensity emitted by the reaction solution, the performance requirements for a single detection component can be reduced.

[0118] In one embodiment, the attenuation component 150 can adopt an attenuation sheet, also known as a neutral density filter. By adopting different attenuation sheets, the incident light intensity of the reaction solution can be reduced according to different attenuation coefficients.

[0119] In another embodiment of the present application, the attenuation component 150 may employ a filter with an attenuation coefficient greater than 1. The filter has a strong absorption or scattering effect on light. The incident light intensity received by the first photodetector and the second photodetector after passing through the filter is lower than that of the light without passing through the filter. The light intensity received by the first photodetector and the second photodetector can be adjusted by selecting different magnitudes of the attenuation coefficient.

[0120] It should be understood that the attenuation component 150 provided on the light incident sides of the first photodetector and the second photodetector may also be composed of a combination of an attenuation sheet and a filter. The attenuation component 150 with any appropriate attenuation coefficient is used to adjust the linear photometric range of the chemical reaction to obtain accurate first and second photon count results.

[0121] In one embodiment, the sample analyzer 100 further includes a scheduling component and a carrier device 131. The carrier device 131 includes at least two placement positions. The scheduling component schedules the sample container between at least two placement positions. The first detection component 1301 and the second detection component 1302 are located at different placement positions. It can be understood that by adding two or more placement positions for placing the sample container on the carrier device 131, each placement position is correspondingly provided with a detection component. For example, after the first detection component 1301 disposed at one of the placement positions measures the light of the sample container loaded with the luminescent reaction solution, the scheduling component is coordinated to move and place the sample container to the next placement position for light measurement by the second detection component 1302, so as to sequentially measure the light of the same sample container and finally obtain the first and second photon count results, thereby improving the detection efficiency.

[0122] In one embodiment, the scheduling component includes at least one of the following:

[0123] A detection disk, with the placement positions provided on the detection disk, and the first detection component 1301 and the second detection component 1302 are arranged at intervals along the circumference of the detection disk;

[0124] A manipulator for grasping the sample container and moving it between each placement position.

[0125] In one embodiment, a detection disk can be used as a scheduling component, and then the same sample container is moved to different photometric positions for photometry. At this time, the first detection component 1301 and the second detection component 1302 in the detection device 130 are circumferentially spaced apart with the detection disk as the center, and one or more placement positions can be set on the detection disk. After the sample container to be measured is placed at the placement position, the detection disk rotates to rotate the sample container to the photometric position where the detection component is set for photometry, and then rotates to the next photometric position for secondary photometry. In another embodiment, a manipulator can also be used as the scheduling component. The manipulator is used to grasp the same sample container and schedule and move the sample container between the placement positions where the detection components are set. Further, the manipulator is configured to be able to rotate horizontally and perform two-dimensional up and down movements.

[0126] In one embodiment, the first detection component 1301 and the second detection component 1302 are circumferentially spaced along the circumference of the same placement position.

[0127] In one embodiment, Figure 10 the controller 140 in is configured to compare the first photon counting result and the second photon counting result with a preset threshold condition, and obtain a target counting result according to the comparison result and the first photon counting result and the second photon counting result.

[0128] Further, the controller 140 can be configured to select one of at least two photon counting results as the target counting result according to the comparison result, or perform a fusion calculation according to the comparison result and the first photon counting result and the second photon counting result to obtain the target counting result.

[0129] Further, obtaining the target counting result according to the comparison result and the first photon counting result and the second photon counting result includes at least one of the following:

[0130] When the first photon counting result or the second photon counting result is less than the first preset threshold, the second photon counting result is used as the target counting result. It can be understood that when the luminescence intensity of the reaction solution is weak and there is no pulse pile-up effect, the controller 140 can be configured to judge the photon counting result using the first preset threshold. If the first photon counting result or the second photon counting result is not affected and the comparison results are both less than the first preset threshold, the second photon counting result can be selected as the target counting result.

[0131] When the first photon counting result or the second photon counting result is greater than or equal to the first preset threshold and less than the second preset threshold, the first photon counting result and the second photon counting result are fused to obtain a target counting result. It should be understood that when the luminescence intensity of the reaction solution is medium, the controller 140 can be configured to use the first preset threshold and the second preset threshold to judge the photon counting result. If the first photon counting result or the second photon counting result is within the range of the first preset threshold and the second preset threshold, the first photon counting result and the second photon counting result can be fused and calculated to obtain a target counting result. Specifically, the fusion calculation is to perform a weighted summation calculation on the first photon counting result and the second photon counting result to obtain a target counting result, where each weighting coefficient in the weighted summation calculation is a preset value or is set according to at least one of the first photon counting result or the second photon counting result. For example, the functional relationship between the first preset threshold and the second preset threshold and each photon counting result can be determined by looking up a table or a curve. The functional relationship can be a weighted average function, and then at least one weighting coefficient in each photon counting result can be determined according to the result of looking up the table or the curve. If the photon counting result is closer to the first preset threshold or the second preset threshold, the corresponding weighting coefficient is larger. On the contrary, if the photon counting result is farther away from the first preset threshold or the second preset threshold, the corresponding weighting coefficient is smaller. In this way, the finally obtained photon counting result is more linear, further improving the accuracy of the photon counting result. It can be understood that in the embodiment of the present application, the situation that triggers the controller 140 to perform the fusion function calculation can be that the first photon counting result or the second photon counting result is between the first preset threshold and the second preset threshold, and the sum of the weighting coefficients corresponding to the first photon counting result and the second photon counting result is 1.

[0132] When the first photon counting result or the second photon counting result is greater than or equal to the second preset threshold, the first photon counting result is used as the target counting result. It should be noted that when the luminescence intensity of the reaction solution is strong, an attenuation component 150 can be arranged on the light incident side of the photodetector of the first detection component 1301 to reduce the influence on the first photon counting result, while the second photon counting result is greater than or equal to the second threshold, resulting in a pulse pile-up effect. Therefore, the controller 140 is configured to select the first photon counting result as the target counting result.

[0133] It should be noted that the first preset threshold and the second preset threshold in the embodiment of the present application can be set according to actual photometric requirements and stored in the controller 140 in advance, so that a more accurate photon counting result can be obtained.

[0134] Refer to Figure 12As shown, an embodiment of the third aspect of the present application provides a photometric device 1100, which includes at least two detection components 1101 and a controller 1102 that are electrically connected to each other. The detection component 1101 includes a photodetector and a detection circuit. The photodetector is configured to receive the optical signal emitted by the reaction solution in the sample container and convert the optical signal into a corresponding electrical signal; the detection circuit is configured to process the electrical signal and calculate the photon count result of the reaction solution; the controller 1102 is configured to obtain the photon count results processed and calculated by each detection component 1101, and obtain the target count result according to multiple photon count results.

[0135] It can be understood that the photon count results are obtained by sequentially detecting the number of photons of the reaction solution in the same sample container by at least two detection components 1101 in the photometric device 1100, and then the controller 1102 obtains the photon count results of each detection component 1101, and obtains the target count result of the reaction solution according to the photon count results of at least two detection components 1101. Among them, the linear ranges of at least two detection components 1101 of the photometric device 1100 at least partially do not overlap, which can avoid the influence of pulse pile-up phenomenon on photon counting when using a single detection component 1101 to detect the high light intensity section, thereby increasing the upper limit of the linear photometric range of the chemical reaction and improving the detection accuracy of the strong light section of the chemical reaction; and by receiving the optical signal emitted by the reaction solution in the same sample container through the photodetectors of at least two detection components 1101, two photon count results can be obtained in a short time, which is beneficial to improving the chemiluminescence detection efficiency of the photometric device. In one embodiment, the photodetector can be configured as a photomultiplier tube that converts a weak optical signal into an electrical signal. In addition, the optical signal is converted into an electrical signal by the photodetector in the detection component 1101 and sent to the detection circuit for processing to obtain the photon count result of the reaction solution in the same sample container, and the upper limit of the linear photometric range of the photometric device can be increased without designing a complex calculation circuit for photon counting processing.

[0136] Furthermore, the photometric device 1100 provided in the embodiments of the present application can be applied to a sample analyzer, such as a chemiluminescence immunoassay analyzer, especially for detecting weak optical signals generated by chemiluminescence of the reaction solution that are not greater than 200 million photons per second. For example, a chemiluminescence instrument is used to detect target components in biological samples, such as blood or urine or body fluids, based on the chemiluminescence principle. Those skilled in the art can understand that the photometric device 1100 can also be applied to a fluorescence analyzer.

[0137] In one embodiment, refer to Figure 13As shown, the sample analyzer 100 is configured to analyze samples, such as a chemiluminescent immunoassay analyzer for analyzing blood samples. The loading device 1200 includes a sample supply unit 1210 and a reagent supply unit 1220. The sample supply unit 1210 is arranged to aspirate the sample to be tested and supply it into the reaction vessel. The reagent supply unit 1220 is arranged to aspirate the immunoassay reagent (such as a labeled antigen or antibody) and the magnetic bead reagent and supply them into the reaction vessel. The chemiluminescent immunoassay analyzer further includes a magnetic separation device 1400, a substrate supply device (not shown), and a transfer device 1500. The magnetic separation device 1400 is arranged to perform a magnetic separation operation on the reaction vessel containing the sample to be tested, the immunoassay reagent, and the magnetic bead reagent. The substrate supply device is arranged to supply the luminescent substrate into the reaction vessel that has completed the magnetic separation operation. The transfer device 1500 is arranged to transfer the reaction vessel that has completed the magnetic separation operation from the magnetic separation device 1500 to the reaction incubation device 1300. The incubated reaction vessel is transferred to the photometric device 1100 for photometry.

[0138] In one embodiment, the chemiluminescent immunoassay analyzer further includes a sample processing system for transporting the test tube containing the sample to be tested to the sampling position and transporting the test tube after sampling to the recovery area. Specifically, the sample processing system includes a sample loading area and a transport track. The sample loading area is used to store the test tubes containing the samples to be tested placed by the user. The transport track is used to transport the test tubes in the sample loading area to the sampling position. Further, the sample processing system also includes a scanning module for scanning the sample barcode to achieve sample identification and management.

[0139] In one embodiment, the sample supply unit may include a sample needle and a driving mechanism. The driving mechanism is used to drive the sample needle to move between the sampling position and the sample adding position. The sample needle aspirates the sample in the sample tube located at the sampling position and injects the aspirated sample into the reaction vessel located at the sample adding position. Here, the sampling position is, for example, the intersection of the movement trajectory of the sample needle and the sample transport trajectory of the transport track. Further, the sample supply unit also includes a cleaning mechanism for cleaning the sample needle. Further, the sample supply unit also includes a dilution position for placing the reaction vessel that has completed automatic dilution and pretreatment, so that the sample needle can aspirate the diluted sample or the pretreated sample in the reaction vessel located at the dilution position to support the corresponding test mode.

[0140] In one embodiment, the chemiluminescent immunoassay analyzer further includes a first manipulator 1600 for transferring the reaction vessel. The first manipulator is configured to be able to move in three dimensions and can grip the reaction vessel. The first manipulator is configured to load a new reaction vessel into the sample adding position so that the sample needle can inject the sample. The first manipulator is also configured to discard the empty reaction cup. The first manipulator is further configured to transfer the reaction vessel between the sample adding position, the reaction incubation device 1300, and the dilution position.

[0141] In one embodiment, the reagent supply unit 1220 includes a reagent tray 1221 and a reagent needle 1222. The reagent tray 1221 is used to place reagent bottles containing immunoassay reagents and magnetic bead reagents and has the function of refrigerating the reagent bottles. The reagent tray 1221 is configured to be rotatable so as to transfer the reagent bottle that needs to aspirate the reagent to the reagent aspiration position. The reagent needle 1222 is used to aspirate the reagent from the reagent bottle located at the reagent aspiration position and discharge the aspirated reagent into the reaction vessel located at the sample addition position. Further, the reagent tray has a mixing mechanism (not shown) for mixing the magnetic bead reagent. The mixing mechanism realizes the mixing of the magnetic bead reagent, for example, by rotating the reagent bottle containing the magnetic bead reagent. In addition, the reagent supply unit 1220 also includes a cleaning mechanism (not shown) for cleaning the reagent needle.

[0142] In one embodiment, the magnetic separation device 1400 has a disk-shaped rotatable magnetic separation disk, a magnetic mechanism, and a temperature control mechanism. The magnetic separation disk is used to drive the reaction vessels therein to sequentially pass through the magnetic mechanism for magnetic separation operations. The temperature control mechanism is used to maintain the temperature of the magnetic separation disk within a preset range.

[0143] In one embodiment, the substrate supply device has a heating mechanism for preheating the luminescent substrate. The substrate supply device is used to inject the preheated luminescent substrate into the reaction vessel that has completed magnetic separation and is located at the substrate injection position, where the substrate injection position is located on the magnetic separation disk.

[0144] In one embodiment, the reaction incubation device 1300 includes a disk-shaped rotatable reaction disk. The photometric device 1100 is arranged on the outer peripheral side of the reaction disk. The reaction vessel after injecting the luminescent substrate is transported into the reaction disk by the transport device 1500. The reaction disk drives the reaction vessel that has been incubated for a certain period of time into the photometric device 1100 to complete photometry.

[0145] In one embodiment, the transport device 1500 is configured as a second manipulator. The second manipulator is used to clamp the reaction vessel and transport the reaction vessel between the reaction disk and the magnetic separation disk. The second manipulator is configured to be able to rotate horizontally and perform two-dimensional up and down movements.

[0146] In one embodiment, the chemiluminescence immunoassay analyzer further includes a mixer 1700 arranged between the reaction disk and the magnetic separation disk. The mixer is configured to perform a mixing operation on the reaction vessel that has already added reagents and samples. The mixer can be configured as a vortex mixer that realizes non-contact mixing, which can effectively avoid cross-contamination.

[0147] In addition, the chemiluminescence immunoassay analyzer further includes a hardware control system (not shown) and software systems running on a hardware board and a PC respectively, so as to control the above-mentioned various devices of the chemiluminescence immunoassay analyzer to work in coordination. The chemiluminescence immunoassay analyzer further includes a mechanical system (not shown) composed of a frame, a front shell, etc., so as to provide support, positioning and protection for the above-mentioned various devices.

[0148] Referring Figure 14 As shown, the embodiment of the fourth aspect of the present application provides a sample detection method, which can be applied to the sample analyzer 100 in the above embodiment, and includes the following steps:

[0149] Step S100: Load the sample to be tested and the reagent into a sample container to form a reaction solution.

[0150] Step S200: Detect the reaction solution in the sample container, and the method is as follows: Step S210: Use multiple detection components to receive the optical signals emitted by the reaction solution in the sample container; Step S220: Convert the optical signals into electrical signals corresponding to the respective detection components; Step S230: Obtain a photon counting result according to the electrical signals.

[0151] Step S300: Obtain the photon counting results of the respective detection components, and obtain a target counting result according to the multiple photon counting results.

[0152] It can be understood that by using multiple detection components to receive the optical signals emitted by the reaction solution in the sample container, then converting the optical signals into electrical signals corresponding to the respective detection components, and obtaining photon counting results according to the electrical signals, wherein different detection components obtain different photon counting results, and the photodetectors of at least two detection components receive the optical signals emitted by the reaction solution in the same sample container, two photon counting results of the reaction solution can be obtained in a short time, which is beneficial to improving the chemiluminescence detection efficiency; furthermore, the photon counting results of the respective detection components are obtained, and a target counting result is obtained according to the multiple photon counting results, wherein the linear ranges of at least two detection components at least partially do not overlap, or the incident light intensities of the photodetectors of at least two detection components are different, which can avoid the influence of pulse pile-up phenomenon on photon counting when using a single detection component to detect the high light intensity section, thereby increasing the upper limit of the linear photometric range of the chemical reaction and improving the detection accuracy for the strong light section of the chemical reaction.

[0153] The detailed working steps of Step S100 to Step S300 in the embodiment of the present application can be referred to Figures 3 to 13As well as the working process of the controller 140 in the sample analyzer shown in any of the above embodiments, and the method steps for the controller 140 in any of the sample analyzer embodiments to execute or control any component in the sample analyzer can all be introduced into the sample detection method of the embodiments of the present application, and will not be elaborated herein one by one.

[0154] The features or combinations of features mentioned in the specification, drawings, and claims above, as long as they are meaningful within the scope of the present invention and do not conflict with each other, can be arbitrarily combined with each other or used alone. The advantages and features described for the photometric device 1100 provided by the present invention are applicable to the sample analyzer 100 and the sample detection method provided by the present invention in a corresponding manner, and vice versa.

[0155] On the other hand, the embodiments of the present application also provide an electronic device, including a memory and a processor. The memory stores a computer program, and when the processor executes the computer program, it implements the sample detection method of any of the above embodiments.

[0156] On the other hand, the embodiments of the present application also provide a computer-readable storage medium. The storage medium stores a computer program, and when the computer program is executed by a processor, it implements the sample detection method of any of the above embodiments.

[0157] On the other hand, the embodiments of the present application also provide a computer program product. The computer program product includes a computer program, and the computer program is stored in a computer-readable storage medium. The processor of the computer device reads the computer program from the computer-readable storage medium, and the processor executes the computer program, so that the computer device executes and implements the sample detection method of any of the above embodiments.

[0158] In several embodiments provided by the present application, it should be understood that the disclosed systems, devices, and methods can be implemented in other ways. For example, the device embodiments described above are merely illustrative. For example, the division of units is only a logical function division. In actual implementation, there may be other division methods. For example, multiple units or components can be combined or integrated into another system, or some features can be ignored or not executed. Another point is that the displayed or discussed couplings or direct couplings or communication connections to each other can be through some interfaces, and the indirect couplings or communication connections of devices or units can be in electrical, mechanical, or other forms.

[0159] The unit described as a separation component may or may not be physically separated, and the component displayed as a unit may or may not be a physical unit, that is, it may be located in one place or may be distributed to multiple network units. Some or all of the units can be selected according to actual needs to achieve the purpose of the solution of this embodiment.

[0160] In addition, each functional unit in various embodiments of the present application can be integrated in a processing unit, or each unit can exist physically alone, or two or more units can be integrated in one unit. The above-mentioned integrated unit can be implemented in the form of hardware or in the form of a software functional unit.

[0161] If the integrated unit is implemented in the form of a software functional unit and sold or used as an independent product, it can be stored in a computer-readable storage medium. Based on such an understanding, the technical solution of the present application, in essence, or the part that contributes to the prior art, or all or part of the technical solution, can be embodied in the form of a software product. This computer software product is stored in a storage medium and includes several instructions for causing a computer device (which can be a personal computer, a server, or a network device, etc.) to execute all or part of the steps of the methods in various embodiments of the present application. The aforementioned storage medium includes: various media such as USB flash drives, mobile hard disks, read-only memory (ROM), random access memory (RAM), magnetic disks, or optical discs that can store program codes.

[0162] It should also be understood that the various embodiments provided in the embodiments of the present application can be combined arbitrarily to achieve different technical effects.

[0163] The above is a specific description of the preferred embodiments of the present application, but the present application is not limited to the above-mentioned embodiments. Those skilled in the art can also make various equivalent deformations or substitutions without departing from the spirit of the present application, and these equivalent deformations or substitutions are all included in the scope defined by the claims of the present application.

Claims

1. A sample analyzer, comprising: A loading device for loading a sample to be tested and a reagent into a sample container; A reaction device for providing a reaction site for the sample container containing the sample to be tested and the reagent, so that the sample to be tested and the reagent in the sample container form a reaction solution; A detection device, including a carrying device and at least two detection components. At least one placement position for placing the sample container is provided in the carrying device. The detection component includes a photodetector and a detection circuit. The photodetector is configured to receive an optical signal emitted by the reaction solution in the sample container at the placement position and convert the optical signal into a corresponding electrical signal. The detection circuit obtains a photon counting result according to the electrical signal; wherein, at least part of the linear ranges of at least two of the detection components do not overlap, and the photodetectors of at least two of the detection components receive the optical signal emitted by the reaction solution in the same sample container; A controller configured to obtain the photon counting results of each of the detection components and obtain a target counting result according to the photon counting results of at least two of the detection components.

2. The sample analyzer according to claim 1, characterized in that: An attenuation component is provided on the light incident side of the photodetectors of at least two of the detection components, and the attenuation coefficients of the attenuation components provided by at least two of the detection components are different; or, an attenuation component is provided on the light incident side of the photodetector of at least one of the detection components and no attenuation component is provided on the light incident side of the photodetector of at least one of the detection components. The detection circuit in the detection component provided with the attenuation component obtains the photon counting result of the photodetector according to the attenuation coefficient of the attenuation component and the electrical signal obtained from the photodetector.

3. The sample analyzer according to claim 2, wherein The attenuation component includes at least one of the following: An attenuation sheet; A filter sheet with an attenuation coefficient greater than 1; The attenuation component composed of a combination of an attenuation sheet and a filter sheet.

4. The sample analyzer according to claim 1, characterized in that, It further includes a scheduling component. The carrying device includes at least two of the placement positions. The scheduling component schedules the sample container between at least two of the placement positions, and at least two of the detection components are located at different placement positions.

5. The sample analyzer according to claim 4, wherein The scheduling component includes at least one of the following: A detection disk, the placement positions are provided on the detection disk, and at least two of the detection components are arranged at intervals along the circumference of the detection disk; A manipulator for grasping the sample container and moving it between each of the placement positions.

6. The sample analyzer according to claim 1, characterized in that, At least two of the detection components are arranged at intervals along the circumference of the same placement position.

7. The sample analyzer according to any one of claims 1 to 6, characterized in that, The controller is configured to compare at least two photon counting results with a preset threshold condition, and obtain the target counting result according to the comparison result and at least two photon counting results.

8. The sample analyzer according to claim 7, wherein, At least two of the detection components include a first detection component and a second detection component, and the light intensity of the incident light of the photodetector of the first detection component is less than the light intensity of the incident light of the photodetector of the second detection component; the controller is configured to compare the photon counting result obtained by the first detection component or the second detection component with the preset threshold condition, and obtain the target counting result according to the comparison result and the photon counting result obtained by the first detection component or the second detection component.

9. The sample analyzer according to claim 8, wherein The obtaining of the target counting result according to the comparison result and the photon counting result obtained by the first detection component or the second detection component includes at least one of the following: When the photon counting result of the first detection component or the second detection component is less than the first preset threshold, using the photon counting result obtained by the second detection component as the target counting result; When the photon counting result of the first detection component or the second detection component is greater than or equal to the first preset threshold and less than the second preset threshold, fusing the photon counting results of the first detection component and the second detection component to obtain the target counting result; When the photon counting result of the first detection component or the second detection component is greater than or equal to the second preset threshold, using the photon counting result obtained by the first detection component as the target counting result.

10. The sample analyzer according to claim 8 or 9, characterized in that, Attenuation components are provided on the light incident sides of the photodetectors of the first detection component and the second detection component, and the attenuation coefficient of the attenuation component of the first detection component is greater than the attenuation coefficient of the attenuation component of the second detection component. The detection circuits in the first detection component and the second detection component obtain the photon counting results of the first detection component and the second detection component according to the electrical signals converted by the photodetectors and the attenuation coefficients; or, the first detection component is not provided with the attenuation component, the second detection component is provided with the attenuation component, and the detection circuit in the second detection component obtains the photon counting result of the second detection component according to the electrical signal converted by the photodetector and the attenuation coefficient of the attenuation component.

11. The sample analyzer according to claim 7, characterized in that, The controller is configured to select one of at least two photon counting results as the target counting result according to the comparison result, or perform a fusion calculation according to the comparison result and at least two photon counting results to obtain the target counting result.

12. The sample analyzer according to any one of claims 1 to 6, characterized in that, The controller is configured to perform a fusion calculation according to each of the photon counting results to obtain the target counting result. The fusion calculation is to perform a weighted summation calculation on each of the photon counting results to obtain the target counting result, where each of the weighting coefficients in the weighted summation calculation is a preset value or is set according to at least one of at least two of the photon counting results.

13. A sample analyzer, characterized in that, Including: A loading device for loading a sample to be tested and a reagent into a sample container; A reaction device for providing a place for reaction and incubation for the sample container containing the sample to be tested and the reagent, so that the sample to be tested and the reagent in the sample container form a reaction solution; A first detection component, the first detection component includes a first photodetector and a first detection circuit, the first photodetector is configured to receive the optical signal emitted by the reaction solution in the sample container and convert the optical signal into a first electrical signal, and the first detection circuit obtains a first photon counting result according to the first electrical signal; A second detection component, the second detection component includes a second photodetector and a second detection circuit, the second photodetector is configured to receive the optical signal emitted by the reaction solution in the sample container and convert the optical signal into a second electrical signal, and the second detection circuit obtains a second photon counting result according to the second electrical signal; wherein, the first photodetector and the second photodetector receive the optical signal emitted by the reaction solution in the same sample container, and the incident light intensity of the first photodetector is less than the incident light intensity of the second photodetector; A controller configured to obtain the first photon counting result and the second photon counting result, and obtain a target counting result according to the first photon counting result and the second photon counting result.

14. The sample analyzer according to claim 13, characterized in that, The second detection component further includes an attenuation component arranged on the light incident side of the second photodetector, and no attenuation component is arranged on the light incident side of the first photodetector; the second detection circuit obtains the second photon counting result according to the attenuation coefficient of the attenuation component and the electrical signal obtained from the second photodetector; Alternatively, the controller is configured to obtain the target counting result according to the first photon counting result, the second photon counting result and the attenuation coefficient of the attenuation component, and the second photon counting result is obtained according to the electrical signal obtained from the second photodetector.

15. The sample analyzer according to claim 13, characterized in that, Attenuation components are arranged on the light incident sides of both the first photodetector and the second photodetector, wherein the first attenuation coefficient of the attenuation component of the first photodetector is less than the second attenuation coefficient of the attenuation component of the second photodetector; the first photon counting result is obtained according to the first attenuation coefficient and the electrical signal obtained from the first photodetector.

16. A photometric device, comprising At least two detection components, the detection components including a photodetector and a detection circuit, the photodetector being configured to receive an optical signal emitted by a reaction solution in a sample container and convert the optical signal into a corresponding electrical signal, the detection circuit obtaining a photon count result according to the electrical signal; wherein, The linear ranges of at least two of the detection components at least partially do not overlap, the reaction solution is formed by a sample to be tested and a reagent, and the photodetectors of at least two of the detection components receive the optical signal emitted by the reaction solution in the same sample container; A controller configured to obtain the photon counting results of each of the detection components and obtain a target counting result according to the multiple photon counting results.

17. A sample detection method, comprising: Loading a sample to be tested and a reagent into a sample container to form a reaction solution; And Detecting the reaction solution in the sample container in the following manner: Multiple detection components are used to receive the optical signals emitted by the reaction solution in the sample container, and respectively convert the optical signals into electrical signals corresponding to each detection component, and a photon counting result is obtained according to the electrical signals. Among them, different detection components obtain different photon counting results, and the photodetectors of at least two detection components receive the optical signals emitted by the reaction solution in the same sample container; Obtain the photon counting results of each detection component, and obtain a target counting result according to the multiple photon counting results; Among them, the linear ranges of at least two detection components at least partially do not overlap, or the incident light intensities of the photodetectors of at least two detection components are different.

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