A acne-removing and repairing composition specially developed for teenagers and its application

Abstract

This invention discloses a acne-repairing and repairing composition specifically developed for adolescents and its application, relating to the field of cosmetic technology. The invention provides an acne-repairing and repairing composition comprising 5-12 parts of guaiac extract, 1-6 parts of eggplant extract, 0.001-0.01 parts of aescin, 0.01-0.1 parts of diosgenin, and 0.01-0.1 parts of asiaticoside. This invention achieves a three-dimensional synergistic effect of "anti-inflammatory, repair, and metabolic regulation" through the synergistic interaction of five components: guaiac extract, eggplant extract, aescin, diosgenin, and asiaticoside, forming a comprehensive management system for acne and post-acne repair in adolescents.

Description

Technical Field

[0001] This invention relates to the field of cosmetic technology, and in particular to an acne-repairing composition specifically developed for teenagers and its application. Background Technology

[0002] Acne is a chronic inflammatory skin disease that is prevalent among adolescents worldwide. Its pathogenesis involves four core pathological links: (1) Androgen-mediated sebaceous gland hyperfunction: The level of androgens (testosterone, dihydrotestosterone) increases during puberty, which stimulates the proliferation of sebaceous glands and excessive secretion of sebum, resulting in oily skin; (2) Abnormal follicular keratinization: Excessive proliferation of keratinocytes at the hair follicle duct, which blocks the pores and forms microcomedones (whiteheads or blackheads), becoming the initial lesions of acne; (3) Microbial community imbalance: The anaerobic environment in the hair follicle promotes the formation of Propionibacterium acnes biofilm, and its metabolites trigger an inflammatory response; (4) Over-activation of immune response: Neutrophil infiltration and the cascade release of pro-inflammatory factors (IL-1α, IL-8, TNF-α) induce the overexpression of matrix metalloproteinases (MMPs), leading to rupture of the hair follicle wall and deep dermal inflammation, ultimately forming inflammatory papules, pustules or scar tissue.

[0003] Current clinical treatment faces the following technical challenges: ① Poor tolerance to topical medications: Although retinoids (adapalene) can inhibit follicular keratosis and IL-6 secretion, their damage to the epidermal barrier function leads patients to discontinue treatment due to erythema and desquamation; ② Limitations of systemic treatment risks: Although oral isotretinoin can inhibit sebaceous gland cell proliferation, it carries risks such as teratogenicity and liver damage, requiring strict contraception and monitoring of liver function; ③ Misconceptions in adjuvant therapy exacerbate pathology: Frequent soap-based cleansing damages the skin's acidic protective film, prompting compensatory increases in sebum secretion; unprofessional acid peels lead to thinning of the stratum corneum and increased sensitivity of TRPV1 receptors, inducing rosacea-like reactions; ④ Deficiencies in patient self-intervention: Misleading social media leads to squeezing of lesions, causing bacterial spread, while the abuse of acne products containing glucocorticoids induces telangiectasia and hormone-dependent dermatitis.

[0004] Therefore, this application is submitted. Summary of the Invention

[0005] Based on this, the purpose of the present invention is to overcome the shortcomings of the prior art and provide an acne-repairing composition specifically for teenagers and its application.

[0006] To achieve the above objectives, the technical solution adopted by the present invention is as follows: an acne-removing and repairing composition, comprising the following components in parts by weight: 5-12 parts of guaiac extract, 1-6 parts of solanum extract, 0.001-0.01 parts of aesculin, 0.01-0.1 parts of diosgenin, and 0.01-0.1 parts of asiaticoside.

[0007] The inventors discovered in their research that asiaticoside can stimulate fibroblast proliferation and collagen synthesis, accelerating epidermal repair and reducing acne scars and pits; diosgenin can reduce deep tissue damage such as acne pits by inhibiting fibrosis pathways, while inducing abnormal cell apoptosis and preventing recurrent inflammation; aesculin promotes local microcirculation and accelerates the excretion of metabolic waste. These three components work synergistically to promote both superficial and deep repair, rapidly advancing skin cell repair. Guaiac extract and solanum extract work synergistically with these three components to further strengthen the skin barrier, forming a three-dimensional repair network from the epidermis to the dermis. Furthermore, the inventors discovered in their actual research that the five components—guaiac extract, solanum extract, asiaticoside, diosgenin, and aesculin—work synergistically to balance sebaceous gland activity, reduce excessive sebum secretion, and prevent clogged pores. They also inhibit lipid peroxidation, reduce abnormal follicular keratinization, and constrict capillaries, thereby regulating the skin's water-oil balance and metabolism, and reducing the conditions for acne formation from the source.

[0008] This invention's acne-repairing composition selects and adds appropriate weight proportions of guaiac wood extract, solanum fruit extract, aescin, diosgenin, and asiaticoside. These five components work synergistically to effectively improve post-acne scars, reduce sebum secretion, and repair the skin barrier, achieving significant effects on acne and post-acne repair in adolescents. This invention achieves a three-dimensional synergistic effect of "anti-inflammatory, repair, and metabolic regulation" through the synergistic interaction of these five components, forming a comprehensive management system for acne and post-acne repair in adolescents.

[0009] Preferably, component A comprises the following ingredients in parts by weight: 8-10 parts of guaiac extract, 2-4 parts of solanum extract, 0.003-0.005 parts of aesculin, 0.02-0.05 parts of diosgenin, and 0.05-0.08 parts of asiaticoside.

[0010] The inventors discovered in their actual research that the weight proportions of the components in the composition provided by this invention affect the performance of the product. When the weight proportions of the components are further selected within the above-mentioned range, the resulting product has a more superior three-dimensional synergistic effect of "anti-inflammatory-repair-metabolic regulation".

[0011] Preferably, the eggplant extract accounts for 20-30% of the total weight of the acne-repairing composition.

[0012] The inventors discovered in their actual research that when the weight percentage of the tomato fruit extract is within the above-mentioned range based on the total weight of the acne-repairing composition, the resulting product exhibits a superior three-dimensional synergistic effect of "anti-inflammatory-repair-metabolic regulation".

[0013] Preferably, the method for preparing the eggplant extract includes the following steps: taking eggplant fruit, drying and pulverizing it, and sieving it to obtain coarse eggplant powder; then adding an extraction solvent for ultrasonic extraction, filtering, concentrating and drying the extract to obtain the eggplant extract; wherein the extraction solvent is a eutectic solvent.

[0014] Preferably, the weight ratio of the extraction solvent to the solanaceous powder is 10-20:1; and / or, the water content of the eutectic solvent is 20-40%; and / or, the ultrasonic extraction temperature is 30-60℃, the ultrasonic extraction time is 20-60 min, and the extraction is performed 1-3 times.

[0015] Preferably, the eutectic solvent is diluted with deionized water to obtain eutectic solvents with different water contents.

[0016] Preferably, the hydrogen bond acceptor of the eutectic solvent is choline chloride, and the hydrogen bond donor of the eutectic solvent is at least one of lactic acid, ethylene glycol, urea, citric acid, and glucose.

[0017] Preferably, the molar ratio of the hydrogen bond acceptor to the hydrogen bond donor is 1:2-4.

[0018] The inventors discovered in their actual research that using different extraction methods to prepare eggplant fruit extract affects the content of flavonoids and polyphenols, such as anthocyanins and chlorogenic acid, in the eggplant fruit extract. Consequently, when eggplant fruit extracts prepared using different extraction methods are added to acne-repairing compositions, the efficacy of the resulting acne-repairing compositions will vary. When the preparation method as described above is selected, the overall effect of the resulting composition is better.

[0019] Furthermore, the present invention provides the application of the acne-repairing composition in the preparation of skin care products.

[0020] Furthermore, the present invention provides a skin care product comprising the aforementioned acne-repairing composition. Preferably, the skin care product comprises any one of toner, lotion, cream, lotion, mask, serum, or spray.

[0021] Preferably, the skin care product comprises the following components by weight percentage: 1-3% acne repair composition, 2-40% cosmetic base, and the balance being water.

[0022] Preferably, the cosmetic base includes at least one of a thickener, a moisturizer, an emulsifier, a preservative, a fragrance, and a pH adjuster.

[0023] Preferably, the skin care product comprises the following components by weight percentage: 1-3% acne-repairing composition, 0.05-0.5% thickener, 3-20% moisturizer, 0.5-10% emulsifier, 0.2-5% preservative, 0.01-0.2% fragrance, 0.05-0.1% pH adjuster, and the balance being water.

[0024] For example, the thickener includes at least one of xanthan gum, carbomer 940, AVC, and high molecular weight cellulose; the humectant includes at least one of glycerin, butylene glycol, and low molecular weight sodium hyaluronate; the emulsifier includes at least one of coconut oil-caprylate / capric acid ester, polydimethylsiloxane, hydrogenated palm kernel oil, cetearyl alcohol, polyglycerol-6 distearate, and polymethylsilsesquioxane; the preservative includes at least one of 1,3-propanediol, 1,2-hexanediol, and p-hydroxyacetophenone; and the pH adjuster includes at least one of arginine and EDTA-2Na.

[0025] Compared to existing technologies, the beneficial effects of this invention are as follows: The acne-repairing composition of this invention selects and adds appropriate weight proportions of guaiac extract, solanum extract, aescin, diosgenin, and asiaticoside. These five components synergistically improve post-acne scars, reduce sebum secretion, and repair the skin barrier, achieving significant effects on acne and post-acne repair in adolescents. This invention achieves a three-dimensional synergistic effect of "anti-inflammatory-repair-metabolic regulation" through the synergistic interaction of these five components, forming a comprehensive management system for acne and post-acne repair in adolescents. Attached Figure Description

[0026] Figure 1 The cell migration diagrams at 6 hours are for the blank control group, comparative example 1, and example 1 in performance test-1.

[0027] Figure 2 Cell migration diagrams at 24 hours for the blank control group, comparative example 1, and example 1 in performance test-1;

[0028] Figure 3 The graphs show the improvement in acne on days 0, 3, and 14 in Application Example 1 of Performance Test-3.

[0029] Figure 4 The graph shows the improvement in acne at 0, 3, and 14 days compared to Application Example 1 in Performance Test-3. Detailed Implementation

[0030] To better illustrate the purpose, technical solution, and advantages of this invention, the invention will be further described below in conjunction with the accompanying drawings and specific embodiments. The purpose is to provide a detailed understanding of the invention, not to limit it. All other embodiments obtained by those skilled in the art without inventive effort are within the scope of protection of this invention. Unless otherwise specified, the experimental reagents and instruments designed in the embodiments and comparative examples of this invention are commonly used reagents and instruments, all of which are commercially available. Unless otherwise specified, the experimental methods used in the embodiments and comparative examples are conventional methods; and unless otherwise specified, the raw materials used in parallel experiments are from the same batch.

[0031] The raw materials of this invention are described below, but are not limited to the following raw materials:

[0032] Guaiac extract: purchased from Shanghai E. En Chemical Technology Co., Ltd.;

[0033] Aesculin: purchased from Yuanye Biotechnology Co., Ltd.;

[0034] Diosgenin: purchased from Anpu Laboratory Technology Co., Ltd.;

[0035] Hydroxyasiaticoside: purchased from Bailingwei Technology Co., Ltd.;

[0036] Licorice extract: purchased from Myriel Biochemical Technology Co., Ltd.;

[0037] Astragalus saponins: purchased from Jingcui Tiancheng Pharmaceutical Technology Co., Ltd.;

[0038] Centella asiatica glycoside: purchased from Bailingwei Technology Co., Ltd.;

[0039] Smilax saponin: purchased from Dest Biotechnology Co., Ltd.;

[0040] Eggplant fruit extract-1: self-made, prepared as follows: cleaned eggplant fruits are dried and pulverized in a 60℃ oven and passed through a 40-mesh sieve to obtain coarse eggplant fruit powder. Then, an extraction solvent is added for ultrasonic extraction, followed by filtration. The extract is concentrated and dried to obtain eggplant fruit extract. The extraction solvent is eutectic solvent-1, and the water content of the eutectic solvent is 30%. The weight ratio of the eutectic solvent to the coarse eggplant fruit powder is 15:1. The ultrasonic extraction is performed for 30 minutes at a temperature of 40℃, and the extraction is repeated twice.

[0041] Solanum fruit extract-2: prepared in-house, the only difference from the preparation method of solanum fruit extract-1 is that the extraction solvent is eutectic solvent-2.

[0042] Solanum fruit extract-3: prepared in-house, the only difference from the preparation method of solanum fruit extract-1 is that the extraction solvent is eutectic solvent-3.

[0043] Solanum fruit extract-4: prepared in-house, the only difference from the preparation method of solanum fruit extract-1 is that the extraction solvent is eutectic solvent-4.

[0044] Solanum fruit extract-5: prepared in-house, the only difference from the preparation method of solanum fruit extract-1 is that the extraction solvent is eutectic solvent-5.

[0045] Solanum fruit extract-6: prepared in-house, the only difference from the preparation method of solanum fruit extract-1 is that the extraction solvent is eutectic solvent-6.

[0046] Solanum fruit extract-7: prepared in-house, the only difference from the preparation method of solanum fruit extract-1 is that the extraction solvent is eutectic solvent-7.

[0047] Solanum Extract-8: Prepared in-house, the only difference from Solanum Extract-1 is that the extraction solvent is eutectic solvent-8.

[0048] Solanum Extract-9: Prepared in-house, the only difference from Solanum Extract-1 is that the extraction solvent is eutectic solvent-9.

[0049] Solanum fruit extract-10: prepared in-house, the only difference from the preparation method of solanum fruit extract-1 is that the extraction solvent is a 60% (v / v) aqueous solution of ethanol.

[0050] The composition and ratio of the eutectic solvent are selected as shown in Table 1. The preparation method of the eutectic solvent is as follows: the hydrogen bond acceptor and the hydrogen bond donor are placed in an 80°C constant temperature water bath and mixed evenly. After cooling to room temperature, they are diluted with deionized water to different water contents to obtain the eutectic solvent.

[0051] Table 1

[0052]

[0053] Examples 1-14 and Comparative Examples 1-11

[0054] The present invention provides an acne-repairing composition in the embodiments and comparative examples. The components and weight parts of the composition are shown in Tables 2 and 3. The preparation method of the composition includes the following steps: mixing each component evenly to obtain the acne-repairing composition.

[0055] Table 2

[0056]

[0057]

[0058] Table 3

[0059]

[0060]

[0061] Application examples and comparative application examples

[0062] Application Examples 1-15, Comparative Application Examples 1-11, and Blank Application Examples of the present invention provide lotions containing acne-repairing and repairing compositions. The components (mass percentage) of the lotions are shown in Table 4. The acne-repairing and repairing compositions used in Application Examples 1-14 are those prepared in Examples 1-14, and the acne-repairing and repairing compositions used in Comparative Application Examples 1-11 are those prepared in Comparative Examples 1-11. For example, the acne-repairing and repairing composition used in Application Example 1 is the one prepared in Example 1; the acne-repairing and repairing composition used in Application Example 2 is the one prepared in Example 2; the acne-repairing and repairing composition used in Application Example 15 is the one prepared in Example 1; and the acne-repairing and repairing composition used in Comparative Application Example 1 is the one prepared in Comparative Example 1. The same logic applies to other examples.

[0063] Table 4

[0064]

[0065]

[0066] The method for preparing the emulsion provided in Application Example 1 is as follows:

[0067] (1) Mix phase A with water and stir. Heat to 80°C and homogenize at 1200 rpm for 5 minutes. After homogenization, keep warm for later use to obtain pre-prepared phase A.

[0068] (2) Mix the B phase, heat it to 80°C, and then homogenize it at 1200 rpm for 5 minutes. After homogenization, keep it warm for later use to obtain the pre-made B phase.

[0069] (3) Mix the C phase and heat it to 60°C to melt it, thus obtaining the pre-made C phase;

[0070] (4) Heat the pre-prepared phase A to 80°C, add the pre-prepared phase B at 300 rpm, stir and mix. Then cool down to 60°C, add the pre-prepared phase C at 300 rpm and stir and mix. Then cool down to below 45°C, add the acne-repairing composition and fragrance and continue stirring for 5 minutes. Finally, add arginine to adjust the pH to 5.0-6.5, then stop stirring, discharge the material, and obtain the emulsion.

[0071] The preparation methods of the emulsions provided in the other application examples, comparative application examples, and blank application examples are consistent with those in application example 1. If the relevant components are not available, they can be omitted.

[0072] Performance Test - 1 Skin Cell Repair Test

[0073] The efficacy of the compositions prepared in Examples 1-14 and Comparative Examples 1-11 was investigated, as follows:

[0074] On a monolayer of adherent cells cultured in an in vitro culture dish or plate, a line is drawn in the central region of cell growth using a pipette tip or other hard object. Cells in the central region are removed, and the cells are cultured again until the predetermined experimental time is reached. The ability of cells at the edge of the scratch to gradually enter the blank area and heal the "scratch" is used to determine the cell's growth and migration ability. This can simulate the wound healing process and is used to evaluate the cell's ability to migrate at the wound site.

[0075] Experimental materials: Cell line: HaCaT (human immortalized keratinocytes), P17, purchased from Beina Biotechnology. Fetal bovine serum: Gibco, DMEM basal medium, phosphate-buffered saline (PBS), penicillin (100 U / mL), streptomycin (100 μg / mL), 0.25% trypsin, cell culture flasks and porous cell culture plates, 0.22 μm microporous filter membrane, wound healing 2-well insert (ibidi, REF: 80209).

[0076] Experimental Procedure: Take out a 12-well plate. Using sterile forceps, fix the 2-well wound healing insert in the center of the corresponding well. Gently press the insert to secure it in the well (the 2-well wound healing insert, ibidi, was purchased from Shanghai Huayasichuang Biotechnology Co., Ltd., catalog number 80209). Inoculate cells at 4.0 × 10⁻⁶ cells / well. 5 70 μL / well was seeded into 2-well wound healing inserts, with 3 replicates per group. Cells were then cultured in an incubator until confluence. The culture plate was removed, and the 2-well wound healing inserts were removed to create "wounds." Exfoliated cells were washed with PBS. The control group was replaced with culture medium containing 0.1% fetal bovine serum (FBS), and the test group was replaced with culture medium containing 0.1% FBS of the test substance. Images were acquired at fixed locations, recording cell migration at 0h, 6h, and 24h.

[0077] Data Analysis: The scratch area of ​​the cell control wells and each sample well was observed from 0h to 6h to 24h. Image analysis was performed using ImageJ software, and the scratch area was measured to calculate the cell migration status. The scratch area at 0h was taken as 100%, and the relative size of the scratch area at each time point in each group was calculated.

[0078] Cell migration rate = (0h scratch area - post-culture scratch area) / 0h scratch area × 100%.

[0079] The results are shown in Table 5.

[0080] Table 5

[0081] Group Cell migration rate (%) at 6 hours 24-hour cell migration rate (%) Blank control group 15.13 40.30 Example 1 34.27 85.22 Example 2 28.79 78.58 Example 3 28.11 77.11 Example 4 27.68 76.65 Example 5 27.04 75.37 Example 6 33.60 83.90 Example 7 26.01 73.11 Example 8 26.05 73.13 Example 9 32.94 83.08 Example 10 25.26 70.86 Example 11 34.25 85.20 Example 12 30.17 77.58 Example 13 30.21 77.30 Example 14 32.89 81.11 Comparative Example 1 17.84 49.27 Comparative Example 2 17.97 49.25 Comparative Example 3 18.67 51.81 Comparative Example 4 18.56 51.79 Comparative Example 5 18.88 51.76 Comparative Example 6 18.80 51.85 Comparative Example 7 19.68 52.76 Comparative Example 8 21.80 53.23 Comparative Example 9 22.70 54.35 Comparative Example 10 22.68 54.31 Comparative Example 11 22.62 54.28

[0082] As shown in the table above, the composition prepared in the embodiments of the present invention has a good effect on promoting skin cell repair, with fast repair speed and good repair effect. The cell migration rate reaches more than 25% in 6 hours and more than 70% in 24 hours. Figure 1 Cell migration diagrams at 6 hours for the blank control group, Comparative Example 1, and Example 1;

[0083] Figure 2 The figures show the 24-hour cell migration of the blank control group, comparative example 1, and example 1 in performance test-1. As can be seen from the figures, the composition prepared in this invention has a good effect on promoting skin cell repair, with fast repair speed and good repair effect.

[0084] As can be seen from the comparison of Examples 1-10, when the eggplant extract prepared by different extraction methods is added to the acne repair composition, the efficacy of the resulting acne repair composition will vary. In particular, the choice of extraction solvent has a significant impact. When a eutectic solvent with choline chloride as the hydrogen bond acceptor and lactic acid as the hydrogen bond donor is used, the effect of promoting skin cell repair is better.

[0085] As can be seen from the comparison of Examples 1 and 11-14, the weight parts of the components in the composition will affect the performance of the product. When the acne repair composition includes the following ingredients in parts by weight: 8-10 parts of guaiac extract, 2-4 parts of solanum extract, 0.003-0.005 parts of aesculin, 0.02-0.05 parts of diosgenin, and 0.05-0.08 parts of asiaticoside, the effect of promoting skin cell repair is better.

[0086] As can be seen from the comparison of Example 1 and Comparative Examples 1-11, the effects of the present invention cannot be achieved when one or two of the components are not added, or when the mass fraction of one component is added to another component without adding the other component.

[0087] Compared with Example 1, when other components were used to replace the components in this invention, although the cell migration rate at 6 hours could reach more than 20%, the cell migration rate at 24 hours was much lower than that in Example 1, and the effect of promoting skin cell repair was not good. The five components of this invention work synergistically and none of them can be omitted.

[0088] Performance Test - 2 Anti-inflammatory Effect Test

[0089] The efficacy of the compositions prepared in Examples 1-14 and Comparative Examples 1-11 was investigated, as follows:

[0090] Experimental materials: The cell line used was mouse macrophage RAW264.7, purchased from the Cell Resource Center of the Institute of Basic Medical Sciences, Chinese Academy of Medical Sciences; fetal bovine serum (FBS, Gibco); DMEM high glucose medium (DMEM, Gibco); dimethyl sulfoxide (DMSO, Sigma); phosphate-buffered saline (PBS, Gibco); thiazolyl blue (MTT, Sigma); mouse tumor necrosis factor-α ELISA kit (Mouse TNF-α ELISA kit, Boster); and mouse interleukin-1β ELISA kit (Mouse IL-1β ELISA kit, Boster).

[0091] The experimental group setup is shown in Table 6. The specific procedures are as follows:

[0092] (1) Inoculation: The cells were seeded into 24-well plates and incubated at 37°C in a 5% CO2 incubator for 24 hours.

[0093] (2) Solution preparation: Prepare the test substance and positive control according to Table 6;

[0094] (3) Sample administration: According to the experimental grouping and concentration settings in Table 6, after the cells in the 24-well plate have grown for 24 hours, samples are administered to the groups, with 3 replicates for each treatment group. Cell culture medium is added to the blank control and negative control groups, and cell culture medium containing the corresponding concentration of sample is added to the sample groups. The cells are then incubated at 37°C in a 5% CO2 incubator for 24 hours.

[0095] (4) LPS induction: After culturing for 24 hours, the culture medium in the plate was aspirated and washed once with PBS. Cell culture medium was added to the blank control group, and cell culture medium containing LPS was added to the negative control group, sample group and positive control group. After culturing for 24 hours in a 5% CO2 incubator at 37°C, the supernatant was collected.

[0096] (5) Detection of inflammatory factor content: Take the cell supernatant from each well and detect the inflammatory factor content of the cells according to the ELISA kit instructions; Inflammatory factor inhibition rate (%) = (average concentration of negative control group - average concentration of sample group) / average concentration of negative control group × 100%.

[0097] (6) Data processing: The data obtained in the experiment were processed and plotted using Excel software. The results are shown in Table 7.

[0098] Table 6

[0099]

[0100] Table 7

[0101]

[0102]

[0103] As shown in the table above, the composition prepared in the embodiments of the present invention has a good anti-inflammatory effect, and the inhibition rate of the inflammatory factor IL-1β reaches more than 50%.

[0104] As can be seen from the comparison of Examples 1-10, the efficacy of the acne-repairing composition varies when different extraction methods are used to prepare the eggplant fruit extract and it is added to the acne-repairing composition. In particular, the choice of extraction solvent has a significant impact. When a eutectic solvent with choline chloride as the hydrogen bond acceptor and lactic acid as the hydrogen bond donor is used, the anti-inflammatory effect is better.

[0105] As can be seen from the comparison of Examples 1 and 11-14, the weight parts of the components in the composition will affect the performance of the product. When the acne repair composition includes the following components in parts by weight: 8-10 parts of guaiac extract, 2-4 parts of solanum extract, 0.003-0.005 parts of aesculin, 0.02-0.05 parts of diosgenin, and 0.05-0.08 parts of asiaticoside, the anti-inflammatory effect is better.

[0106] As can be seen from the comparison of Example 1 and Comparative Examples 1-11, the effects of the present invention cannot be achieved when one or two of the components are not added, or when the mass fraction of one component is added to another component without adding one component; and when other components are used to replace the components in the present invention, the anti-inflammatory effect is also poor. The five components of the present invention work synergistically and none of them can be omitted.

[0107] Performance Testing - 3 Human Efficacy Testing

[0108] The efficacy of lotions containing acne-repairing and repairing compositions was explored in Application Examples 1-15, compared with Application Examples 1-11 and a blank application example, as follows:

[0109] This effect test examines the effects of emulsions prepared in the application example, control application example, and blank application example on improving skin TEWL, acne count, oil content, and skin brightness.

[0110] The specific testing method is as follows:

[0111] According to the "Cosmetic Safety Technical Specifications" (2015), volunteers with sensitive skin aged 18-50 (lactate stinging score >3) were selected for the experiment and randomly divided into 27 groups of 5 people each. Each volunteer had at least 2-3 noticeable pimples. Under normal circumstances, volunteers used the product on their entire face for 14 days. Every morning and evening, after cleansing and moisturizing, volunteers applied an appropriate amount of lotion to their face and massaged it in until absorbed. Layering was allowed on pimple areas. The product was used on the entire face twice a day, morning and evening. Follow-up visits were conducted on days 3 and 14. On the day of the follow-up visit, volunteers washed their faces with water without applying any product and sat quietly for 20 minutes in an air-conditioned room with a temperature of 21±1℃ and humidity of 50±10%.

[0112] The improvement of facial acne indicators was quantified and photographed using instruments, including acne count (using VISIA and doctor assessment), transepidermal water loss (TEWL) measured by Tewameter™ Hex, oil content measured by Sebumeter SM 815, and brightness measured by Skin-Colorimeter CL400. The improvement rate before and after use was calculated as |mean value before use - mean value after use| / mean value before use × 100%; the results are shown in Table 8.

[0113] Table 8

[0114]

[0115]

[0116]

[0117] As can be seen from the table above, the emulsion prepared by the application example of the present invention has a good three-dimensional synergistic effect of "anti-inflammatory-repair-metabolic regulation". Figure 3 The graphs show the improvement in acne on days 0, 3, and 14 of application example 1; Figure 4 To compare the acne improvement charts of Application Example 1 at 0, 3, and 14 days, it can be seen that the emulsion prepared in this invention has a very good effect on improving acne.

[0118] As can be seen from the comparison of application examples 1-10, when different extraction methods are used to prepare the eggplant fruit extract and it is added to the acne repair composition, the efficacy of the resulting acne repair composition will vary. In particular, the choice of extraction solvent has a significant impact during the extraction process. When a eutectic solvent with choline chloride as the hydrogen bond acceptor and lactic acid as the hydrogen bond donor is used, the three-dimensional synergistic effect of "anti-inflammatory-repair-metabolic regulation" is better.

[0119] As can be seen from the comparison of Application Examples 1 and 11-14, the weight parts of the components in the composition will affect the performance of the product. When the acne repair composition includes the following ingredients in parts by weight: 8-10 parts of guaiac extract, 2-4 parts of solanum extract, 0.003-0.005 parts of aesculin, 0.02-0.05 parts of diosgenin, and 0.05-0.08 parts of asiaticoside, the three-dimensional synergistic effect of "anti-inflammatory-repair-metabolic regulation" is better.

[0120] As can be seen from the comparison of Application Example 1 and Comparative Application Examples 1-11, the effects of the present invention cannot be achieved when one or two of the components are not added, or when the mass fraction of one component is added to another component without adding one component. Furthermore, when other components are used to replace the components in the present invention, the three-dimensional synergistic effect of "anti-inflammatory-repair-metabolic regulation" is not good. The five components of the present invention work synergistically with each other and none of them can be omitted.

[0121] Finally, it should be noted that the above embodiments are only used to illustrate the technical solutions of the present invention and are not intended to limit the scope of protection of the present invention. Although the present invention has been described in detail with reference to preferred embodiments, those skilled in the art should understand that modifications or equivalent substitutions can be made to the technical solutions of the present invention without departing from the essence and scope of the technical solutions of the present invention.

Claims

1. An acne-removing and repairing composition, characterized in that, The product comprises the following components in parts by weight: 5-12 parts guaiac extract, 1-6 parts solanum fruit extract, 0.001-0.01 parts aesculin, 0.01-0.1 parts diosgenin, and 0.01-0.1 parts asiaticoside. The preparation method of the solanum fruit extract includes the following steps: drying and pulverizing solanum fruit, sieving to obtain solanum fruit coarse powder; then adding an extraction solvent for ultrasonic extraction, filtering, concentrating and drying the extract to obtain the solanum fruit extract; the extraction solvent is a eutectic solvent.

2. The acne-removing and repairing composition as described in claim 1, characterized in that, It includes the following ingredients in parts by weight: 8-10 parts guaiac extract, 2-4 parts solanum fruit extract, 0.003-0.005 parts aesculin, 0.02-0.05 parts diosgenin and 0.05-0.08 parts asiaticoside.

3. The acne-removing and repairing composition as described in claim 1, characterized in that, The weight percentage of the eggplant extract is 20-30% based on the total weight of the acne-repairing composition.

4. The acne-removing and repairing composition as described in claim 1, characterized in that, The weight ratio of the extraction solvent to the crude solanaceous powder is 10-20:1; and / or, the water content of the eutectic solvent is 20-40%; and / or, the ultrasonic extraction temperature is 30-60℃, the ultrasonic extraction time is 20-60 min, and the extraction is performed 1-3 times.

5. The acne-removing and repairing composition as described in claim 1, characterized in that, The hydrogen bond acceptor of the eutectic solvent is choline chloride, and the hydrogen bond donor of the eutectic solvent is at least one of lactic acid, ethylene glycol, urea, citric acid, and glucose.

6. The acne-removing and repairing composition as described in claim 5, characterized in that, The molar ratio of the hydrogen bond acceptor to the hydrogen bond donor is 1:2-4.

7. The use of the acne-repairing composition as described in any one of claims 1-6 in the preparation of skin care products.

8. A skin product, characterized in that, The skin care products include the acne-repairing composition as described in any one of claims 1-6.

9. The skin product as described in claim 8, characterized in that, The skin care product comprises the following components by weight percentage: 1-3% acne repair composition, 2-40% cosmetic base, and the remainder water.

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