Traditional Chinese medicine composition for preventing and treating sheep lung adenoma disease as well as preparation and application of traditional Chinese medicine composition
By using traditional Chinese medicine compositions such as Huangguan, the problem of no effective vaccine and therapeutic drugs for sheep lung adenomatosis was solved, and the effect of effectively preventing and treating sheep lung adenomatosis was achieved, which reduced the mortality rate and drug resistance risk, and had low cost and low residue biosafety.
Patent Information
- Application Number
- CN202510554920.6
- Authority / Receiving Office
- CN · China
- Patent Type
- Applications(China)
- Current Assignee / Owner
- Filing Date
- 2025-04-29
- Publication Date
- 2025-06-27
AI Technical Summary
There is no effective vaccine or therapeutic drug for sheep lung adenoma, which leads to a high post-mortem mortality rate and causes huge economic losses to farmers.
Provided is a traditional Chinese medicine composition, including Huangguan, Bossus, Liugui, Chuanpu, Yunling, Diwen, stem, fragrant fruit, bitter almond, winter serpentium, and sweet grass, which reduces the risk of drug resistance through multi-target synergistic effects and is used to prevent and treat sheep lung adenomatosis.
This traditional Chinese medicine composition can effectively prevent and treat pulmonary adenomatosis in sheep, reduce mortality, reduce drug resistance risk, and has less damage to liver and kidney function, simple operation and low cost.
Smart Images
Figure CN120204308A_ABST
Abstract
Description
Technical Field
[0001] The present invention belongs to the field of biomedicine, and particularly relates to a traditional Chinese medicine composition for preventing and treating ovine pulmonary adenomatosis. Background Art
[0002] Ovine pulmonary adenomatosis (OPA), also known as ovine lung cancer or "driving sheep disease", is a chronic, progressive, contagious pulmonary tumor disease caused by Jaagsiekte sheep retrovirus (JSRV). The Jaagsiekte sheep retrovirus belongs to the retrovirus family and can induce the transformation and deterioration of type II alveolar epithelial cells and non-ciliated bronchiolar epithelial cells in sheep. The main symptoms of diseased sheep are coughing, running nose, dyspnea, progressive emaciation of the body, adenomatous hyperplasia of alveolar and bronchiolar epithelium, forming multiple papillary cystadenomas, and finally resulting in death.
[0003] Diseased sheep excrete the virus during asthma or coughing, and the nearby susceptible sheep inhale it through the respiratory tract, causing infection. It can also be vertically transmitted to lambs through the placenta. The incubation period is 2 months to 2 years, generally 6 - 9 months. After the incubation period, the diseased sheep begins to show respiratory symptoms, with an accelerated breathing rate, mainly shallow and rapid breathing. When the body does not get enough oxygen, the diseased sheep often straightens its head, expands its nostrils, and breathes forcefully to increase the oxygen intake. As the disease progresses, the diseased sheep begins to show symptoms such as coughing, the head straightening and drooping, and thin secretions constantly flowing out of the nostrils.
[0004] According to literature reports, the incidence rate of infected flocks is 3% - 18%, and can reach 30% severely. The mortality rate after onset is 100%. Clinical symptoms are mostly seen in adult sheep aged 2 - 4 years, and occasionally in goats. The research group of the present invention found that the incidence rate of this disease in some sheep farms can reach more than 85%. At present, there is no vaccine for prevention and treatment of this disease, and the application of existing antiviral drugs has little effect. Most of the diseased sheep farms carry out whole - flock culling, causing huge economic losses to farmers.
[0005] Traditional veterinary medicine focuses on syndrome differentiation and treatment, and inhibits virus proliferation and improves lung function by overall regulating the body's immunity, providing a new approach for reference in the treatment of this disease. Summary of the Invention
[0006] Based on the above - mentioned technical problems, the present invention provides a traditional Chinese medicine composition for preventing and treating ovine pulmonary adenomatosis and its preparation and application. The traditional Chinese medicine composition has the advantages of good effect on preventing and treating ovine pulmonary adenomatosis, multi - target, low residue, good biological safety, reducing the risk of drug resistance through multi - pathway synergistic action, and causing less damage to liver and kidney functions. Specifically, it includes the following contents:
[0007] First aspect, the present invention provides a traditional Chinese medicine composition for preventing and treating ovine pulmonary adenomatosis, and the traditional Chinese medicine composition comprises the following components in parts by mass: 15-20 parts of Huang Can, 15-20 parts of Jia Su, 8-10 parts of Liu Gui, 8-10 parts of Chuan Po, 8-10 parts of Yun Ling, 8-10 parts of Di Wen, 5-9 parts of Geng Cao, 5-9 parts of Xiang Guo, 5-8 parts of bitter apricot kernels, 5-8 parts of Dong Zhu, 5-8 parts of Tian Cao.
[0008] Preferably, the traditional Chinese medicine composition comprises the following components in parts by mass: 17 parts of Huang Can, 17 parts of Jia Su, 10 parts of Liu Gui, 10 parts of Chuan Po, 10 parts of Yun Ling, 10 parts of Di Wen, 6 parts of Geng Cao, 5 parts of Xiang Guo, 5 parts of bitter apricot kernels, 5 parts of Dong Zhu, 5 parts of Tian Cao.
[0009] Second aspect, the present invention provides an application of the traditional Chinese medicine composition described in the first aspect in preparing a drug for inhibiting ovine pulmonary adenomatosis virus.
[0010] Third aspect, the present invention provides an application of the traditional Chinese medicine composition described in the first aspect in preparing a drug for preventing or treating ovine pulmonary adenomatosis.
[0011] Fourth aspect, the present invention provides an application of the traditional Chinese medicine composition described in the first aspect in preparing a feed additive for sheep.
[0012] Fifth aspect, the present invention provides an application of the traditional Chinese medicine composition described in the first aspect in preparing sheep feed.
[0013] Sixth aspect, the present invention provides a preparation method of the traditional Chinese medicine composition described in the first aspect, and the preparation method comprises:
[0014] (1) Sorting: removing impurities in each component;
[0015] (2) Crushing: weighing and mixing each component and then crushing into fine powder of 200 meshes;
[0016] (3) Soaking, decocting and extracting: adding water with a weight 5 times that of the drug to the crushed medicinal powder for soaking and then decocting and extracting, and separating the medicinal liquid from the medicinal residues
[0017] (4) Secondary soaking, decocting and extracting: adding water with a weight 5 times that of the original drug to the medicinal residues in step (3) for soaking and then decocting and extracting, and separating the medicinal liquid from the medicinal residues; the decocted medicinal liquid;
[0018] (6) Concentrating.
[0019] Preferably, the soaking time is 3 hours.
[0020] Preferably, the decocting and extracting is decocting for 2 hours after boiling.
[0021] Preferably, the concentrated liquid is concentrated to a weight of 1 times that of the original drug powder.
[0022] The beneficial effects of the present invention are as follows: Based on the traditional veterinary medicine's emphasis on syndrome differentiation and treatment, the present invention provides a traditional Chinese medicine composition for preventing and treating ovine pulmonary adenomatosis; the traditional Chinese medicine composition includes yellow ginseng, false perilla, willow cinnamon, magnolia bark, tuckahoe, earthworm, licorice, fructus carpesii, bitter almond, atractylodes rhizome, and sweet herb; the traditional Chinese medicine composition can effectively prevent and treat the occurrence of ovine pulmonary adenomatosis, changing the current situation that there is no vaccine and no effective treatment drug after the occurrence of ovine pulmonary adenomatosis; it can be administered directly by oral administration or mixed with feed, with simple operation and low cost; since the present invention is a traditional Chinese herbal medicine preparation, it avoids the generation of problems such as drug resistance, greatly reducing the probability of treatment failure; the present invention is a pure traditional Chinese medicine compound, with low residue in sheep and good biological safety. BRIEF DESCRIPTION OF THE DRAWINGS
[0023] Figure 1 Serous nasal discharge of diseased sheep;
[0024] Figure 2 Autopsy of lungs of diseased sheep;
[0025] Figure 3 Detection results of ovine pulmonary adenomatosis virus, where M is DL2000 marker; 1 is negative control; 1-17 are part of the test samples;
[0026] Figure 4 Detection results of ovine pulmonary adenomatosis virus in some sheep after treatment with the traditional Chinese medicine composition. DETAILED DESCRIPTION OF THE EMBODIMENTS
[0027] The traditional Chinese medicine composition for preventing and treating ovine pulmonary adenomatosis in the following embodiments includes the following components in parts by weight: 15-20 parts of yellow ginseng, 15-20 parts of false perilla, 8-10 parts of willow cinnamon, 8-10 parts of magnolia bark, 8-10 parts of tuckahoe, 8-10 parts of earthworm, 5-9 parts of licorice, 5-9 parts of fructus carpesii, 5-8 parts of bitter almond, 5-8 parts of atractylodes rhizome, and 5-8 parts of sweet herb.
[0028] Example 1 Preparation of the traditional Chinese medicine composition
[0029] (1) Weigh 34 kg of yellow ginseng, 34 kg of false perilla, 20 kg of willow cinnamon, 20 kg of magnolia bark, 20 kg of tuckahoe, 20 kg of earthworm, 12 kg of licorice, 10 kg of fructus carpesii, 10 kg of bitter almond, 10 kg of atractylodes rhizome, and 10 kg of sweet herb according to the proportion, totaling 200.00 kg;
[0030] (2) Sort and crush into fine powder of 200 meshes;
[0031] (3) Soak the crushed drug powder in 5 times the weight of the drug with clear water at room temperature for 3 hours, decoct for 2 hours, then separate the medicinal liquid and the drug residue, and keep the medicinal liquid for standby;
[0032] (4) Add the drug residue separated in step (2) to clear water 5 times the weight of the original drug powder, soak at room temperature for 1 hour, decoct for 2 hours, and separate the medicinal liquid.
[0033] (5) Combine the medicinal liquids extracted twice, concentrate the mixture into a concentrated liquid with a weight of 1 times the original drug powder, and filter.
[0034] For easy storage in the later stage, 20.00 g of potassium sorbate and 20.00 g of sodium diacetate preservatives can be added to the concentrated liquid, and then it is sub-packed to obtain the finished product.
[0035] Prevention and treatment of the traditional Chinese medicine composition on ovine pulmonary adenomatosis in Example 2
[0036] 1. Animal selection
[0037] (1) Disease incidence
[0038] A large number of sheep (Hu sheep) were raised in a ranch in Ning County, Gansu Province. After the weather suddenly turned cold in April 2024, a total of 178 sheep showed respiratory symptoms such as coughing and runny nose. At first, individual sheep had coughs, and as time went by, the number of sheep showing symptoms such as coughing, runny nose, and dyspnea increased. The diseased sheep were mainly concentrated in lambs aged 2 - 8 months. After treatment with antibiotics such as tylosin and florfenicol and antiviral moroxydine, the condition did not improve, and as many as 28 sheep died within 2 weeks.
[0039] (2) Clinical symptoms
[0040] The body temperature of the diseased sheep was normal, but they were listless, emaciated, coughing, dyspneic, with abdominal breathing. Especially after strenuous exercise or long - distance driving, their breathing rate was faster than that of healthy sheep. When the hindquarters were lifted, that is, during the "wheelbarrow test", a large amount of nasal fluid flowed out, accompanied by respiratory moist rales, and serous nasal mucus flowed out (as Figure 1 shown).
[0041] (3) Autopsy changes
[0042] The whole lung was enlarged, adhered to the thoracic cavity, and there were round or oval light - red to gray - white nodules on the lung surface, with edema and fleshy changes. The lung tissue between the nodules was dark red, the nodules were relatively solid in texture, and the cut surface was dry (as Figure 2 shown).
[0043] (4) Pathogen detection
[0044] 150 nasal swabs were collected from symptomatic diseased sheep, and viral RNA was extracted according to the instructions of the viral nucleic acid extraction kit (AG Company, USA). Using the extracted viral RNA as a template, cDNA was synthesized according to the instructions of the reverse transcription kit (AG Company, USA). The reaction conditions for reverse transcription were 37 °C for 45 min and 85 °C for 5 s. The reaction system was 4 μl of 5×Evo M-MLV RT Master Mix, 10 μl of Total RNA, and 6 μl of RNase free water. Using the primers synthesized by Beijing Aoke Dingsheng Biotechnology Co., Ltd. (sequence 5'to3', F: CAgACCTCCTATgAAATgTT, R: CCTgTATAATCggAgTCAAT), with the reverse-transcribed cDNA as a template, pre-denaturation was carried out at 95 °C for 5 min, denaturation at 94 °C for 30 s, annealing at 55 °C for 30 s, extension at 72 °C for 1 min, and after 30 cycles, extension was carried out at 72 °C for 5 min. The reaction system was 12.5 μl of 2×Pro Taq Master Mix (dye plus), 1 μl of template cDNA, 1 μl of JSRVF, 1 μl of JSRVR, and 9.5 μl of RNase free water. The amplified products were electrophoresed on a 1% agarose gel at 80 V for 20 min, and after electrophoresis, photographs were taken using a gel ultraviolet analyzer.
[0045] The results are as Figure 3 shown. The results showed that the expected target fragment of the ovine pulmonary adenocarcinoma virus could be amplified from the nasal mucus of the diseased sheep collected. The proportion of positive sheep in the flock was 73.3% (101 / 150), the proportion of suspected positive sheep was 15.3% (23 / 150), and the proportion of negative sheep was 17.3% (26 / 150). Combining the disease incidence, clinical symptoms and autopsy lesions, it was diagnosed as ovine pulmonary adenocarcinoma virus disease.
[0046] 2. Treatment with traditional Chinese veterinary medicine
[0047] The traditional Chinese medicine composition prepared in Example 1 was used to treat the positive and suspected positive (124 sheep) after PCR detection, and the negative (26 sheep) after PCR detection were given preventive medication. Among them, the treatment dosage was 70 ml / sheep for drinking and feeding, and the preventive dosage was halved. The medication was continued for 7 days. The number of dead sheep treated with the medication decreased to 4 on the 2nd day after medication, to 2 on the 3rd day, to 1 on the 5th day, and no more deaths occurred since the 6th day. The symptoms of coughing, wheezing and running nose in the treated sheep decreased day by day after medication and were basically controlled since the 5th day. Within one week after stopping the medication, none of the surviving sheep showed coughing and wheezing again, and the health status of the flock was good. On the 15th day after medication, nasal mucus of the sheep was collected again, and the PCR test results of the remaining sheep showed that all were negative. Figure 4 The detection results of some sheep are shown in
[0048] Use of the traditional Chinese medicine composition for preventing and treating ovine pulmonary adenomatosis in the preparation of a medicament for preventing and treating ovine pulmonary adenomatosis.
[0049] Example 3 Clinical trial
[0050] (1) In a farm in Yongchang, Gansu Province, there were 879 sheep in one pen (lake) sheep shed. More than 80% of them showed listlessness, emaciation, cough, dyspnea, abdominal breathing. Especially after strenuous exercise or long-distance driving, their breathing frequency was faster than that of healthy sheep. When conducting the "wheelbarrow test", a large amount of nasal discharge flowed out, accompanied by respiratory moist rales, and serous nasal mucus flowed out. The diseased sheep mainly concentrated in the age group of 2 - 8 months. After pathological dissection of the dead sheep, it was shown that the whole lung was enlarged, adhered to the thoracic cavity, and there were round or oval light red to grayish white nodules on the lung surface, with edema and meat-like changes. The lung tissue between the nodules was dark red, the nodules were relatively solid in texture, and the cut surface was dry. By collecting nasal swabs, extracting RNA, and applying PCR and electrophoresis, the results showed that the expected target fragment of ovine pulmonary adenomatosis virus could be amplified from the nasal mucus of the diseased sheep. Combining clinical symptoms, pathological dissection and virus detection, it was determined to be ovine pulmonary adenomatosis virus infection. The traditional Chinese medicine composition prepared in Example 1 above was administered to the diseased sheep at a dose of 70 mL / sheep by feeding, once a day for 7 consecutive days. After 4 days of medication, the symptoms of cough and asthma were alleviated. After 7 days, the spirit of the entire lamb flock returned to normal, the number of coughing and asthmatic sheep decreased significantly, and the condition was effectively controlled. Observed until the 15th day, 1 sheep died, and a small number of sheep (22 sheep) showed occasional cough and runny nose symptoms. The cure rate was (697 / 721) 96.67%, and the effective rate was 99.86%; the nasal fluid of the cured sheep was collected and tested for virus again, and all were negative.
[0051] (2) In a breeding cooperative in Sunan, Gansu Province, 98 sheep had cough and asthma. Using the above method for detection, it was diagnosed as ovine pulmonary adenomatosis virus infection. The traditional Chinese medicine prepared in Example 1 above was administered to the diseased lambs at a dose of 70 mL / sheep by feeding, once a day for 7 consecutive days. On the 4th day after medication, the symptoms of 70% of the diseased sheep were significantly alleviated. On the 7th day, the condition of the sheep flock was controlled. During this period, 2 sheep died. Observed until the 15th day, nasal fluid was collected for virus detection and the cure rate and effective rate were counted. The sheep with negative virus detection (83 / 98) accounted for 84.69%. The cure rate was 84.69% and the effective rate was 97.96%.
[0052] (3) In a certain farm in Lintan County, Gannan Prefecture, Gansu Province, 70 sheep suffered from cough and asthma. Using the above method, they were detected and diagnosed as infected with ovine pulmonary adenoma virus. The prepared product in Example 1 above was administered to the diseased lambs at a dose of 70 mL / sheep by feeding once a day for 7 consecutive days. Since the 3rd day, the spirit and cough and asthma conditions of the lamb flock have been significantly improved. By the 6th day, the cough and asthma symptoms of the flock have disappeared and the disease has been controlled. Observation continued until the 15th day, and the virus was detected and the cure rate and effective rate were statistically calculated. The sheep with negative virus detection (58 / 70) accounted for 82.86%, the cure rate was 82.86%, and the effective rate was 100%.
[0053] The foregoing is only the preferred embodiment of the present invention and is not intended to limit the present invention. Although the present invention has been described in detail with reference to the foregoing embodiments, those skilled in the art can still modify the technical solutions described in the foregoing embodiments or perform equivalent replacements for some of the technical features. Any modification, equivalent replacement, improvement, etc. made within the spirit and principle of the present invention shall be included within the protection scope of the present invention.
Claims
1. A Chinese medicine composition for preventing and treating sheep pulmonary adenoma, characterized in that: The traditional Chinese medicine composition comprises the following components in parts by weight: 15-20 parts of Rhizoma Corydalis, 15-20 parts of Rhizoma Perillae, 8-10 parts of Rhizoma Cinnamomi, 8-10 parts of Rhizoma Puerariae, 8-10 parts of Rhizoma Poriae, 8-10 parts of Rhizoma Dioscoreae, 5-9 parts of Rhizoma Cyperi, 5-9 parts of Rhizoma Cibotii, 5-8 parts of Rhizoma Amygdaluse, 5-8 parts of Rhizoma Atractylodis Macrocephalae, and 5-8 parts of Rhizoma Ligustici Alba.
2. The Chinese medicine composition according to claim 1, characterized in that The Chinese medicine composition comprises the following components in parts by mass: 17 parts of Rhizoma Corydalis, 17 parts of Rhizoma Perillae, 10 parts of Rhizoma Cinnamomi, 10 parts of Rhizoma Puerariae, 10 parts of Rhizoma Poriae, 10 parts of Rhizoma Dioscoreae, 6 parts of Rhizoma Cyperi, 5 parts of Rhizoma Cibotii, 5 parts of Rhizoma Amygdaluse, 5 parts of Rhizoma Atractylodis Macrocephalae, and 5 parts of Rhizoma Ligustici Alba.
3. Use of the Chinese medicine composition as claimed in claim 1 or 2 in the preparation of drugs for inhibiting ovine pulmonary adenomatosis virus.
4. Use of the Chinese medicine composition as claimed in claim 1 or 2 in the preparation of a drug for preventing or treating sheep pulmonary adenoma.
5. Use of the Chinese medicine composition as claimed in claim 1 or 2 in the preparation of sheep feed additives.
6. Use of the Chinese medicine composition as claimed in claim 1 or 2 in preparing sheep feed.
7. The method for preparing the Chinese medicine composition according to claim 1 or 2, characterized in that: The preparation method comprises: (1) Sorting: removing impurities from each component; (2) Grinding: weigh and mix the components and then grind them into 200 mesh fine powder; (3) Soaking and decoction extraction: Add 5 times the weight of the drug to the crushed powder and then decoct and extract to separate the drug solution and drug residue. (4) secondary soaking and decoction extraction: adding 5 times the weight of the original drug to the drug residue in step (3) and then decocting and extracting, separating the drug solution and the drug residue; drug decoction; (6) Concentration.
8. The preparation method according to claim 7, characterized in that: The soaking time is 3 hours.
9. The preparation method according to claim 7, characterized in that: The decoction extraction is performed by boiling and then decocting for 2 hours.
10. The preparation method according to claim 7, characterized in that: The concentrated liquid is concentrated to 1 times the weight of the original drug powder.