Centrifugal device for stem cell exosome processing

Abstract

The invention discloses a centrifugal device for stem cell exosome processing, and relates to the technical field of centrifugal processing.The centrifugal device comprises a machine shell mechanism, the machine shell mechanism comprises a mounting shell, a control panel is fixedly connected to the surface of the mounting shell, and a bin cover is rotationally connected to one side of the top of the mounting shell; and the alternating pulse type driving mechanism comprises a motor, one end of a rotating shaft of the motor is fixedly connected with a first bevel gear, the bottom of the motor is fixedly connected with the inner wall of the mounting shell, and the motor is electrically connected with the control panel. According to the multi-layer nested centrifugal bin, the motor directly drives the outermost centrifugal bin to conduct centrifugation at an ultra-high rotating speed, and when the outer centrifugal bin drives the inner centrifugal bin to conduct rotation centrifugation at a low speed through the gear set, cell culture fluid is placed in the innermost centrifugal bin, namely the slowest-speed centrifugal bin; and after large-size impurities are removed, the materials directly enter the centrifugal bin with the rotating speed higher than that of the outer layer, multi-stage centrifugation can be completed at a time, and the centrifugal efficiency is higher.

Description

Technical Field

[0001] The present invention relates to the technical field of centrifugal processing, and particularly relates to a centrifugal device for processing stem cell exosomes. Background Art

[0002] Stem cell exosomes are nanoscale vesicles (30 - 150 nanometers in diameter) secreted by stem cells, carrying active components such as proteins, nucleic acids, and lipids, and can serve as key mediators of cell - cell communication. They regulate the functions of recipient cells by delivering biomolecules and have multiple effects such as promoting tissue repair (such as myocardial regeneration, skin healing), regulating immunity (inhibiting inflammatory responses), and anti - fibrosis.

[0003] Currently, the centrifugation of stem cell exosomes requires multiple centrifugations to achieve the desired purity. The particle size of stem cell exosomes is usually 30 - 150 nm, and the density is about 1.13 - 1.19 g / mL. The cell culture medium often contains: large - sized impurities such as cell debris, apoptotic bodies (diameter > 200 nm); medium - sized impurities such as microvesicles (diameter 100 - 1000 nm), protein aggregates; small - sized impurities such as free proteins, nucleic acid fragments. It is difficult to separate all impurities of different sizes and densities in one centrifugation, and different types of impurities need to be removed step by step through multiple centrifugations, resulting in poor centrifugation efficiency. Summary of the Invention

[0004] The present invention provides a centrifugal device for processing stem cell exosomes to solve the problems raised in the above - mentioned background art.

[0005] To solve the above - mentioned technical problems, the technical solution adopted by the present invention is: A centrifugal device for processing stem cell exosomes includes a housing mechanism. The housing mechanism includes a mounting shell, and a control panel is fixedly connected to the surface of the mounting shell. One side of the top of the mounting shell is rotatably connected to a chamber cover; an alternating pulse - type driving mechanism, which includes a motor. One end of the motor shaft is fixedly connected to a first helical gear, and the bottom of the motor is fixedly connected to the inner wall of the mounting shell. The motor is electrically connected to the control panel; a gradually - decreasing transmission mechanism. The gradually - decreasing driving mechanism includes a second friction wheel. The top of the second friction wheel is fixedly connected to a driving shaft, and the top of the driving shaft is fixedly connected to a first rotating disk; a multi - stage eddy - current centrifugation mechanism, which includes a mounting disk. The inside of the mounting disk is fixedly connected to the side of the rotating disk, and a filter cylinder is arranged on the top of the mounting disk.

[0006] The further improvement of the technical solution of the present invention lies in that: the housing mechanism further includes a centrifugal chamber, which is arranged inside the mounting shell. A liquid inlet pipe is fixedly connected to the inside of the chamber cover, and a liquid inlet hopper is fixedly connected to one end of the liquid inlet pipe.

[0007] A further improvement of the technical solution of the present invention is that one end of the liquid inlet pipe away from the liquid inlet bucket is fixedly connected to a conical diverter cover through a support rod, and a liquid outlet pipe with a built-in solenoid valve is provided at the bottom of the inner cavity of the centrifugal chamber, one end of the liquid outlet pipe extends to the outside of the mounting shell, and the solenoid valve inside the liquid outlet pipe is electrically connected to the control panel.

[0008] A further improvement of the technical solution of the present invention is that the alternating pulse drive mechanism also includes a second bevel gear, the surface of the second bevel gear is meshed with the surface of the first bevel gear, one side of the second bevel gear is rotatably connected to a fixing member, and the top of the fixing member is fixedly connected to the inner wall of the mounting shell.

[0009] A further improvement of the technical solution of the present invention is that: a transmission shaft is movably connected inside the fixing part, a sliding groove is provided on the surface of the transmission shaft, the surface of the transmission shaft meshes with the sliding groove on the surface of the transmission shaft, the two ends of the transmission shaft are fixedly connected with a first friction wheel, the surface of the first friction wheel overlaps the surface of the second friction wheel, and a toggle ring is fixedly connected to the surface of the transmission shaft away from the second bevel gear.

[0010] A further improvement of the technical solution of the present invention is that: a driving member is overlapped on the surface of the toggle ring, and the end of the driving member away from the toggle ring is slidably connected to a slide rail, the top of the slide rail is fixedly connected to the inner wall of the mounting shell, one side of the driving member is fixedly connected to an electric telescopic rod, the surface of the electric telescopic rod is fixedly connected to the inside of the fixing member, and the electric telescopic rod is electrically connected to the control panel.

[0011] A further improvement of the technical solution of the present invention is that the step-by-step gradual transmission mechanism also includes a first rotating seat, the bottom of the first rotating seat is fixedly connected to the bottom of the centrifugal chamber, the top of the first rotating seat is rotatably connected to the bottom of the first rotating disk, and the top of the first rotating disk is provided with a gradual transmission unit.

[0012] A further improvement of the technical solution of the present invention is that the gradual transmission unit includes a first gear with a built-in connecting shaft, the first gear is fixedly connected to the top of the first rotating disk through the built-in connecting shaft, the surface of the first gear is meshed with a second gear, the interior of the second gear is fixedly connected to the rotating shaft, the surface of the upper end of the rotating shaft is fixedly connected to a third gear, the number of teeth of the third gear is less than the number of teeth of the second gear, the lower end of the rotating shaft is rotatably connected to the top of the rotating disk, the surface of the third gear is meshed with a fourth gear, and the interior of the fourth gear is fixedly connected to the connecting shaft.

[0013] A further improvement of the technical solution of the present invention lies in that: a second rotating disk is fixedly connected to the top of the connecting shaft, a second sliding groove is slidably connected to the bottom of the second rotating disk, the bottom of the second sliding groove is fixedly connected to the top of the first rotating disk, the number of teeth of the fourth gear is greater than that of the first gear, and three sets of gradual transmission units are provided.

[0014] A further improvement of the technical solution of the present invention lies in that: the multi-stage eddy current centrifugal mechanism further includes a filter element, the filter element is arranged inside the filter cylinder, spiral guide vanes are fixedly connected to the inner wall of the filter cylinder, and the surface of the spiral guide vanes adopts a nano-level hydrophilic coating.

[0015] Due to the adoption of the above technical solution, the technical progress achieved by the present invention compared with the prior art is: The present invention provides a centrifugal device for processing stem cell exosomes. Through a multi-layer nested centrifugal chamber, the motor directly drives the outermost centrifugal chamber to centrifuge at an ultra-high speed. When the outermost centrifugal chamber drives the inner centrifugal chamber to rotate and centrifuge at a lower speed through a gear set, the cell culture solution is placed in the innermost centrifugal chamber, that is, the slowest centrifugal chamber. After removing large-size impurities, it directly enters the outer centrifugal chamber with a faster rotation speed, and multi-stage centrifugation can be completed in one time, with higher centrifugation efficiency. At the same time, spiral guide vanes are added to the inner wall of the centrifugal chamber, and the surface of the vanes adopts a nano-level hydrophilic coating. During centrifugation, the sample forms a rotating eddy under the guidance of the spiral guide vanes, accelerating the sedimentation of exosomes to the chamber wall. The spiral guide vanes break the laminar flow state of traditional centrifugation, increase the collision probability between exosomes and impurities through the eddy current effect, and at the same time utilize the synergy of centrifugal force and guiding effect to shorten the migration path of exosomes, further improving the centrifugation efficiency. The pulsed centrifugation mode adopts intermittent pulsed centrifugation, that is, a short low-speed or stationary period is inserted after the high-speed centrifugation stage, and the pulse interval can be dynamically adjusted according to the sedimentation characteristics of exosomes, using the inertia effect to promote the aggregation and stratification of exosomes, and improving the centrifugation efficiency again. And the double-chamber centrifugation mode makes full use of the pulse interval time, enabling the same power source to drive two centrifugal mechanisms to operate alternately, with a higher single-treatment volume and higher energy utilization rate. Brief Description of the Drawings

[0016] Figure 1 It is the front view structural schematic diagram of the present invention; Figure 2 It is the structural schematic diagram of the present invention with the lid open; Figure 3 It is the structural schematic diagram of the lower end of the installation shell of the present invention; Figure 4 It is the structural schematic diagram of the inside of the installation shell of the present invention; Figure 5 It is the structural schematic diagram of the alternating pulse drive mechanism and the multi-stage eddy current centrifugal mechanism of the present invention; Figure 6It is a schematic diagram of the structure of the alternating pulse drive mechanism of the present invention; Figure 7 It is a schematic diagram of the structure of the multi-stage vortex centrifugal mechanism of the present invention in a disassembled state; Figure 8 It is a bottom view structural schematic diagram of the step-by-step slow transmission mechanism of the present invention in an exploded state; Figure 9 It is a schematic diagram of the structure of the step-by-step slow transmission mechanism of the present invention in an exploded state; Figure 10 It is a schematic cross-sectional structure diagram of a multi-stage vortex centrifugal mechanism of the present invention; Figure 11 It is a schematic cross-sectional structure diagram of the step-by-step gradual transmission mechanism of the present invention.

[0017] In the figure: 11, mounting shell; 12, control panel; 13, compartment cover; 14, centrifugal chamber; 15, liquid inlet pipe; 16, liquid inlet bucket; 17, conical diversion cover; 18, liquid outlet pipe; 21, motor; 22, first bevel gear; 23, second bevel gear; 24, fixing member; 25, transmission shaft; 26, first friction wheel; 27, toggle ring; 28, driving member; 29, slide rail; 210, electric telescopic rod; 31, second friction wheel; 32, driving shaft; 33, first rotating disk; 34, first rotating seat; 35, first gear; 36, second gear; 37, third gear; 38, rotating shaft; 39, fourth gear; 310, connecting shaft; 311, second rotating disk; 312, second sliding groove; 41, mounting disk; 42, filter cartridge; 43, filter element; 44, spiral guide vane. DETAILED DESCRIPTION

[0018] The present invention is further described in detail below in conjunction with embodiments: Example 1

[0019] like Figures 1-11 As shown, the present invention provides a centrifugal device for processing stem cell exosomes, including a casing mechanism, the casing mechanism includes a mounting shell 11, a control panel 12 is fixedly connected to the surface of the mounting shell 11, and a compartment cover 13 is rotatably connected to one side of the top of the mounting shell 11; an alternating pulse drive mechanism, the alternating pulse drive mechanism includes a motor 21, one end of the rotating shaft of the motor 21 is fixedly connected to a first bevel gear 22, the bottom of the motor 21 is fixedly connected to the inner wall of the mounting shell 11, and the motor 21 is electrically connected to the control panel 12; a step-by-step gradual transmission mechanism, the gradual drive mechanism includes a second friction wheel 31, the top of the second friction wheel 31 is fixedly connected to a driving shaft 32, and the top of the driving shaft 32 is fixedly connected to a first rotating disk 33; a multi-stage vortex centrifugal mechanism, the multi-stage vortex centrifugal mechanism includes a mounting disk 41, the interior of the mounting disk 41 is fixedly connected to the side of the rotating disk, and a filter cartridge 42 is arranged on the top of the mounting disk 41.

[0020] In this embodiment, the user introduces the sample to be processed into the innermost filter cartridge 42 in the centrifugal chamber 14 through the liquid inlet hopper 16 and the liquid inlet pipe 15. The conical diverter cover 17 at the end of the liquid inlet pipe 15 can make the sample evenly dispersed into the filter cartridge 42, avoid local sample aggregation, and ensure the stability of the centrifugal process. At the same time, the built-in solenoid valve of the liquid outlet pipe 18 is controlled by the control panel 12 and remains closed in the initial state to prevent sample leakage. Subsequently, after the centrifugation program is set on the control panel 12, the motor 21 is started, and the rotating shaft of the motor 21 drives the first bevel gear 22 to rotate. The first bevel gear 22 and the second bevel gear 23 are meshed with each other, thereby driving the second bevel gear 23 to rotate. The second bevel gear 23 is driven to rotate. The transmission shaft 25 is driven to rotate by the fixing member 24, and the first friction wheels 26 at both ends of the transmission shaft 25 overlap with the second friction wheels 31, so as to transmit power to the second friction wheels 31. At the same time, the electric telescopic rod 210 is periodically extended and retracted under the control of the control panel 12, driving the driving member 28 to slide along the slide rail 29. The driving member 28 periodically pushes the dial ring 27 to cause the transmission shaft 25 to produce axial displacement, thereby changing the contact between the first friction wheel 26 and different second friction wheels 31. This process enables the two second friction wheels 31 to obtain power alternately, and at the same time provides a pulsed driving force for the two sets of step-by-step transmission mechanisms, effectively simulating the fluid environment in the body and reducing the damage of exosomes during the centrifugation process.

[0021] Example 2

[0022] like Figures 1-11As shown, on the basis of Example 1, the present invention provides a technical solution: preferably, the casing mechanism also includes a centrifugal chamber 14, the centrifugal chamber 14 is arranged inside the mounting shell 11, the interior of the chamber cover 13 is fixedly connected with a liquid inlet pipe 15, one end of the liquid inlet pipe 15 is fixedly connected with a liquid inlet bucket 16, and one end of the liquid inlet pipe 15 away from the liquid inlet bucket 16 is fixedly connected with a conical diversion cover 17 through a support rod, and a liquid outlet pipe 18 with a built-in electromagnetic valve is provided at the bottom of the inner cavity of the centrifugal chamber 14, one end of the liquid outlet pipe 18 extends to the outside of the mounting shell 11, and the electromagnetic valve inside the liquid outlet pipe 18 is electrically connected to the control panel 12, and the alternating pulse drive mechanism also includes a second bevel gear 23, the surface of the second bevel gear 23 is meshed with the surface of the first bevel gear 22, and one side of the second bevel gear 23 is rotatably connected with a fixing member 24, and the top of the fixing member 24 is connected to the mounting shell 11. The inner wall of the shell 11 is fixedly connected, and the interior of the fixing member 24 is movably connected with a transmission shaft 25. A slide groove is provided on the surface of the transmission shaft 25, and the surface of the transmission shaft 25 meshes with the slide groove on the surface of the transmission shaft 25. The two ends of the transmission shaft 25 are fixedly connected with a first friction wheel 26, and the surface of the first friction wheel 26 overlaps the surface of the second friction wheel 31. The surface of the transmission shaft 25 away from the second bevel gear 23 is fixedly connected with a toggle ring 27, and the surface of the toggle ring 27 overlaps with a driving member 28. The end of the driving member 28 away from the toggle ring 27 is slidably connected with a slide rail 29, and the top of the slide rail 29 is fixedly connected to the inner wall of the mounting shell 11, and one side of the driving member 28 is fixedly connected to an electric telescopic rod 210, and the surface of the electric telescopic rod 210 is fixedly connected to the interior of the fixing member 24, and the electric telescopic rod 210 is electrically connected to the control panel 12.

[0023] In this embodiment, when the second friction wheel 31 rotates, the first rotating disk 33 is driven to rotate around the first rotating seat 34 through the driving shaft 32, and the gradual transmission unit on the top of the first rotating disk 33 starts to work. Take one of the three groups of gradual transmission units as an example: the first gear 35 rotates synchronously with the first rotating disk 33, and the second gear 36 meshing with the first gear 35 rotates accordingly. The second gear 36 drives the third gear 37 to rotate through the rotating shaft 38. Since the number of teeth of the second gear 36 is less than the number of teeth of the first gear 35, the speed of the second gear 36 will be higher than that of the first gear 35. The third gear 37 is meshed with the fourth gear 39, and the fourth gear 39 drives the connecting shaft 310 and the second rotating disk 311 to rotate. Because the number of teeth of the fourth gear 39 is greater than the number of teeth of the first gear 35, the speed of the fourth gear 39 is less than that of the first gear 35. Under the action of the multi-stage gear transmission, the speed of the second rotating disk 311 is gradually reduced, and the operation is more stable, so that it can drive multiple filter cartridges 42 to increase the speed step by step from the inside to the outside.

[0024] Example 3

[0025] like Figures 1-11As shown, on the basis of Example 1, the present invention provides a technical solution: preferably, the step-by-step gradual transmission mechanism also includes a first rotating seat 34, the bottom of the first rotating seat 34 is fixedly connected to the bottom of the inner cavity of the centrifugal chamber 14, the top of the first rotating seat 34 is rotatably connected to the bottom of the first rotating disk 33, and the top of the first rotating disk 33 is provided with a gradual transmission unit, the gradual transmission unit includes a first gear 35 with a built-in connecting shaft, the first gear 35 is fixedly connected to the top of the first rotating disk 33 through the built-in connecting shaft, the surface of the first gear 35 is meshed with a second gear 36, the interior of the second gear 36 is fixedly connected to a rotating shaft 38, the surface of the upper end of the rotating shaft 38 is fixedly connected to a third gear 37, and the number of teeth of the third gear 37 is less than that of the second gear. The number of teeth of the fourth gear 39 is greater than the number of teeth of the first gear 35. The gradual transmission unit is provided with three groups. The multi-stage vortex centrifugal mechanism also includes a filter element 43. The filter element 43 is provided inside the filter cartridge 42. The inner wall of the filter cartridge 42 is fixedly connected with a spiral guide vane 44. The surface of the spiral guide vane 44 is coated with a nano-scale hydrophilic coating.

[0026] In this embodiment, the second rotating disk 311 drives the mounting disk 41 to rotate, and the filter cartridge 42 on the mounting disk 41 rotates at a high speed accordingly. The sample to be processed gradually enters the outer filter cartridge 42 from the innermost layer under the action of centrifugal force. Under the gradually increasing centrifugal speed of the different filter elements 43 in each layer, the filter element 43 performs multi-layer filtration on the sample to remove impurities of different sizes at a time. At the same time, the spiral guide plate 44 on the inner wall of the filter cartridge 42 guides the sample to form a spiral ascending or descending vortex. The nano-scale hydrophilic coating on the surface of the spiral guide plate 44 can reduce the surface tension between the sample and the guide plate, so that the sample flows The sample forms a rotating vortex under the guidance of the spiral guide vane 44, which accelerates the exosomes to settle to the cavity wall. The spiral guide vanes break the laminar flow state of traditional centrifugation and increase the collision probability of exosomes and impurities through the vortex effect. At the same time, the synergy of centrifugal force and guide effect is used to shorten the migration path of exosomes and further improve the centrifugal efficiency. After the centrifugation is completed, the built-in solenoid valve of the liquid outlet pipe 18 is opened through the control panel 12 to collect the supernatant or precipitate after separation. The collection process can control the liquid outlet speed and time through the control panel 12 according to the experimental requirements to ensure the collection of high-purity stem cell exosomes.

[0027] The working principle of the centrifugal device for processing stem cell exosomes is described in detail below.

[0028] likeFigures 1-11As shown, the user introduces the sample to be processed into the innermost filter cartridge 42 in the centrifugal chamber 14 through the liquid inlet hopper 16 and the liquid inlet pipe 15. The conical diverter cover 17 at the end of the liquid inlet pipe 15 can make the sample evenly dispersed into the filter cartridge 42 to avoid local sample aggregation and ensure the stability of the centrifugal process. At the same time, the built-in solenoid valve of the liquid outlet pipe 18 is controlled by the control panel 12 and remains closed in the initial state to prevent sample leakage. After the centrifugation program is set on the control panel 12, the motor 21 is started, and the rotating shaft of the motor 21 drives the first bevel gear 22 to rotate. The first bevel gear 22 and the second bevel gear 23 are meshed with each other, thereby driving the second bevel gear 23 to rotate. The second bevel gear 23 drives the transmission shaft 25 to rotate through the fixing member 24. The first friction wheels at both ends of the transmission shaft 25 26 overlaps with the second friction wheel 31, thereby transmitting power to the second friction wheel 31. At the same time, the electric telescopic rod 210 is periodically extended and retracted under the control of the control panel 12, driving the driving member 28 to slide along the slide rail 29. The driving member 28 periodically pushes the toggle ring 27 to cause the transmission shaft 25 to produce axial displacement, thereby changing the contact between the first friction wheel 26 and different second friction wheels 31. This process enables the two second friction wheels 31 to obtain power alternately, and at the same time provides a pulse driving force for the two sets of step-by-step slow transmission mechanisms, effectively simulating the fluid environment in the organism and reducing the damage of the exosomes during the centrifugation process. When the second friction wheel 31 rotates, the first rotating disk 33 is driven to rotate around the first rotating seat 34 through the driving shaft 32, and the slow transmission unit on the top of the first rotating disk 33 The element starts to work, taking one of the three groups of gradual transmission units as an example: the first gear 35 rotates synchronously with the first rotating disk 33, and the second gear 36 meshing with the first gear 35 rotates accordingly, and the second gear 36 drives the third gear 37 to rotate through the rotating shaft 38. Since the number of teeth of the second gear 36 is less than the number of teeth of the first gear 35, the speed of the second gear 36 will be higher than that of the first gear 35, and the third gear 37 is meshed with the fourth gear 39, and the fourth gear 39 drives the connecting shaft 310 and the second rotating disk 311 to rotate. Because the number of teeth of the fourth gear 39 is greater than that of the first gear 35, the speed of the fourth gear 39 is lower than that of the first gear 35. Under the action of the multi-stage gear transmission, the speed of the second rotating disk 311 is gradually reduced, and the operation is more stable, so that It can drive multiple filter cartridges 42 to increase the speed step by step from the inside to the outside. The second rotating disk 311 drives the mounting disk 41 to rotate, and the filter cartridges 42 on the mounting disk 41 rotate at high speed. The sample to be processed gradually enters the outer filter cartridge 42 from the innermost layer under the action of centrifugal force. Under the gradually increasing centrifugal speed of different filter elements 43 in each layer, the filter element 43 performs multi-layer filtration on the sample to remove impurities of different sizes at a time. At the same time, the spiral guide plate 44 on the inner wall of the filter cartridge 42 guides the sample to form a spiral ascending or descending vortex. The nano-scale hydrophilic coating on the surface of the spiral guide plate 44 can reduce the surface tension between the sample and the guide plate, making the sample flow smoother. Under the guidance of the spiral guide plate 44, the sample forms a rotating vortex to accelerate the sedimentation of exosomes to the cavity wall.The spiral guide vane breaks the laminar flow state of traditional centrifugation, increases the collision probability between exosomes and impurities through the eddy current effect, and at the same time utilizes the synergy of centrifugal force and guiding action to shorten the migration path of exosomes, further improving the centrifugation efficiency. After centrifugation, the solenoid valve built into the liquid outlet pipe 18 is opened through the control panel 12 to collect the separated supernatant or precipitate. During the collection process, the liquid outlet speed and time can be controlled through the control panel 12 according to experimental requirements to ensure the collection of high-purity stem cell exosomes.

[0029] The present invention has been generally and exhaustively described above. However, based on the present invention, some modifications or improvements can be made, which are obvious to those of ordinary skill in the art. Therefore, any modifications or improvements made without departing from the spirit and idea of the present invention fall within the protection scope of the present invention.

Claims

1. A centrifugal device for processing stem cell exosomes, characterized in that: include A casing mechanism, the casing mechanism comprising a mounting shell (11), a control panel (12) being fixedly connected to a surface of the mounting shell (11), and a compartment cover (13) being rotatably connected to one side of a top of the mounting shell (11); An alternating pulse drive mechanism, the alternating pulse drive mechanism comprising a motor (21), one end of a rotating shaft of the motor (21) being fixedly connected to a first bevel gear (22), a bottom of the motor (21) being fixedly connected to an inner wall of a mounting shell (11), and the motor (21) being electrically connected to a control panel (12); A step-by-step slow-down transmission mechanism, the step-by-step slow-down drive mechanism comprising a second friction wheel (31), the top of the second friction wheel (31) being fixedly connected to a drive shaft (32), and the top of the drive shaft (32) being fixedly connected to a first rotating disk (33); A multi-stage vortex centrifugal mechanism comprises a mounting plate (41), the interior of the mounting plate (41) being fixedly connected to the side of a rotating plate, and a filter cartridge (42) being arranged on the top of the mounting plate (41).

2. A centrifugal device for processing stem cell exosomes according to claim 1, characterized in that: The housing mechanism further comprises a centrifugal chamber (14), the centrifugal chamber (14) being arranged inside the mounting shell (11), the interior of the compartment cover (13) being fixedly connected to a liquid inlet pipe (15), one end of the liquid inlet pipe (15) being fixedly connected to a liquid inlet hopper (16).

3. A centrifugal device for processing stem cell exosomes according to claim 2, characterized in that: One end of the liquid inlet pipe (15) away from the liquid inlet bucket (16) is fixedly connected to a conical flow divider (17) via a support rod; a liquid outlet pipe (18) with a built-in electromagnetic valve is provided at the bottom of the inner cavity of the centrifugal chamber (14); one end of the liquid outlet pipe (18) extends to the outside of the mounting shell (11); and the electromagnetic valve inside the liquid outlet pipe (18) is electrically connected to the control panel (12).

4. A centrifugal device for processing stem cell exosomes according to claim 1, characterized in that: The alternating pulse drive mechanism further comprises a second bevel gear (23), the surface of the second bevel gear (23) meshing with the surface of the first bevel gear (22), one side of the second bevel gear (23) being rotatably connected to a fixing member (24), the top of the fixing member (24) being fixedly connected to the inner wall of the mounting shell (11).

5. A centrifugal device for processing stem cell exosomes according to claim 4, characterized in that: The fixing member (24) is movably connected to a transmission shaft (25) inside, a sliding groove is provided on the surface of the transmission shaft (25), the surface of the transmission shaft (25) meshes with the sliding groove on the surface of the transmission shaft (25), both ends of the transmission shaft (25) are fixedly connected to first friction wheels (26), the surface of the first friction wheel (26) overlaps the surface of the second friction wheel (31), and a toggle ring (27) is fixedly connected to the surface of the transmission shaft (25) at one end away from the second bevel gear (23).

6. A centrifugal device for processing stem cell exosomes according to claim 5, characterized in that: A driving member (28) is overlapped on the surface of the toggle ring (27); an end of the driving member (28) away from the toggle ring (27) is slidably connected to a slide rail (29); the top of the slide rail (29) is fixedly connected to the inner wall of the mounting shell (11); one side of the driving member (28) is fixedly connected to an electric telescopic rod (210); the surface of the electric telescopic rod (210) is fixedly connected to the inside of the fixing member (24); and the electric telescopic rod (210) is electrically connected to the control panel (12).

7. A centrifugal device for processing stem cell exosomes according to claim 1, characterized in that: The step-by-step gradual transmission mechanism further comprises a first rotating seat (34), the bottom of the first rotating seat (34) being fixedly connected to the bottom of the inner cavity of the centrifugal chamber (14), the top of the first rotating seat (34) being rotatably connected to the bottom of the first rotating disk (33), and the top of the first rotating disk (33) being provided with a gradual transmission unit.

8. A centrifugal device for processing stem cell exosomes according to claim 1, characterized in that: The gradual transmission unit comprises a first gear (35) with a built-in connecting shaft, the first gear (35) is fixedly connected to the top of the first rotating disk (33) via the built-in connecting shaft, a second gear (36) is meshed on the surface of the first gear (35), a rotating shaft (38) is fixedly connected inside the second gear (36), a third gear (37) is fixedly connected to the surface of the upper end of the rotating shaft (38), the number of teeth of the third gear (37) is smaller than the number of teeth of the second gear (36), the lower end of the rotating shaft (38) is rotatably connected to the top of the rotating disk, a fourth gear (39) is meshed on the surface of the third gear (37), and a connecting shaft (310) is fixedly connected inside the fourth gear (39).

9. A centrifugal device for processing stem cell exosomes according to claim 8, characterized in that: The top of the connecting shaft (310) is fixedly connected to a second rotating disk (311), the bottom of the second rotating disk (311) is slidably connected to a second sliding groove (312), the bottom of the second sliding groove (312) is fixedly connected to the top of the first rotating disk (33), the number of teeth of the fourth gear (39) is greater than the number of teeth of the first gear (35), and three groups of the gradual transmission unit are provided.

10. A centrifugal device for processing stem cell exosomes according to claim 1, characterized in that: The multi-stage vortex centrifugal mechanism further comprises a filter element (43), wherein the filter element (43) is arranged inside the filter cartridge (42), and a spiral guide plate (44) is fixedly connected to the inner wall of the filter cartridge (42), wherein the surface of the spiral guide plate (44) is provided with a nano-scale hydrophilic coating.

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