Strain preservation tube and strain preservation tube cover thereof

Abstract

The invention discloses a strain preservation tube and a strain preservation tube cover. The strain preservation tube comprises a preservation tube body, and the bottom of the preservation tube body is detachably connected with a preservation tube bottom; a reset pipe is fixedly connected to the side face of the storage pipe body, a pressure relief pipe is inserted into the reset pipe, an exhaust valve is arranged at the end, located on the outer side of the reset pipe, of the pressure relief pipe, an air outlet hole is formed in the end, inserted into the reset pipe, of the pressure relief pipe, and a side pulling plate is arranged on the portion, located between the exhaust valve and the reset pipe, of the outer surface of the pressure relief pipe. According to the strain preservation pipe and the strain preservation pipe cover thereof, through cooperative arrangement of the adding pipe, the pressing rod, the sealing plug, the one-way feeding mechanism, the feeding pipe and the communicating pipe, it can be guaranteed that the interior of the preservation pipe body is always isolated from the external environment in the process of adding a protective agent or other reagents in the using process; only the reagent can enter the storage tube body, so that external infectious microbes are prevented from entering the storage tube body to pollute strains cultured in the storage tube body in the reagent adding process.

Description

Technical Field

[0001] The present invention relates to the technical field of microorganism preservation, and specifically to a strain preservation tube and a tube cap thereof. Background Art

[0002] Plant pathogenic bacteria and fungi are important experimental materials for microbiology and molecular biology research. The pathogenic bacteria obtained by isolation and purification are prone to mutation or loss during subculture and activation. The mutation of strains is related to the speed of their metabolism. External conditions such as low temperature, dryness, and air isolation are the main factors reducing the metabolic ability of strains. Strain preservation is to adjust these three factors to make the strains in a semi-dormant state and maintain their vitality and biological characteristics within a certain period of time for subsequent research. There are many methods for strain preservation, including subculture refrigerator preservation method, liquid nitrogen preservation method, cryogenic freezing method, etc. The subculture refrigerator preservation method does not require special equipment. The process of the subculture refrigerator preservation method is as follows: After the strain grows well in the liquid medium, protective agents such as glycerol, paraffin oil, skimmed milk, etc. are added, and then it can be stored at -4°C for a long time. The subculture refrigerator preservation method has low cost and is simple and easy to operate, and is widely used in strain preservation. Strain culture and preservation require a preservation tube. When adding the protective agent, the tube cap needs to be opened, and then the protective agent is added into the preservation tube, which reduces the preservation efficiency. Moreover, the opening of the tube cap will also increase the probability of the strain being contaminated. And the preservation tube has a certain depth, and its bottom is relatively difficult to clean, which is not convenient for subsequent reuse. Summary of the Invention

[0003] (I) Technical Problems to be Solved Aiming at the deficiencies of the prior art, the present invention provides a strain preservation tube and a tube cap thereof, which have the advantages of being able to add protective agents without opening the tube cap, etc., and solve the above problems.

[0004] (II) Technical Solutions To achieve the above object, the present invention provides the following technical solution: a strain preservation tube and a tube cap for the strain preservation tube, including a preservation tube body, the bottom of the preservation tube body is detachably connected with a preservation tube bottom; a reset tube is fixedly connected to the side of the preservation tube body, a pressure relief tube is inserted into the reset tube, an exhaust valve is provided at one end of the pressure relief tube located outside the reset tube, an air outlet hole is provided at one end of the pressure relief tube inserted into the reset tube, and a side pull plate is provided on the outer surface of the pressure relief tube between the exhaust valve and the reset tube; a tube cap is detachably connected to the top of the preservation tube body, a feeding tube is fixedly connected to the center position of the upper surface of the tube cap, a pressing rod is inserted into the feeding tube, a sealing plug is fixedly connected to one end of the pressing rod inserted into the feeding tube, an opening is provided on the tube cap corresponding to the feeding tube, a one-way feeding mechanism is provided on the lower surface of the tube cap corresponding to the feeding tube, and a feeding pipe is provided on the upper surface of the tube cap on one side of the feeding tube, and the feeding pipe is communicated with the feeding tube through a communicating pipe.

[0005] Preferably, a threaded connection groove is provided at the bottom of the preservation tube body, and a bottom sealing ring is fixedly connected to the top of the inner surface of the threaded connection groove.

[0006] Preferably, a threaded connection ring is fixedly connected to the upper surface of the preservation tube bottom, the thread on the threaded connection ring is adapted to the thread in the threaded connection groove, and the top of the threaded connection ring abuts against the bottom of the bottom sealing ring.

[0007] Preferably, a reset spring is sleeved on the outer surface of the section of the pressure relief tube inserted into the reset tube, a reset plate is fixedly connected to the outer surface of the pressure relief tube, one end of the reset spring abuts against the side of the reset plate, and the other end abuts against the outer surface of the preservation tube body.

[0008] Preferably, the exhaust valve is a one-way valve, and the gas flow direction in the exhaust valve is from the inside of the preservation tube body to the outside. A sealing gasket is fixedly connected to one end of the pressure relief tube inserted into the preservation tube body, and the sealing gasket abuts against the side wall of the inner surface of the preservation tube body.

[0009] Preferably, a retaining ring is fixedly connected to the side wall of the inner surface of the feeding tube, and the position of the retaining ring is between the sealing plug and the communicating pipe.

[0010] Preferably, the one-way feeding mechanism includes a sealing tube, an installation ring is fixedly connected to the side wall of the inner surface of the sealing tube, a stretching spring is fixedly connected to the bottom of the installation ring, a feeding plug is fixedly connected to the bottom of the stretching spring, and the top of the feeding plug abuts against the bottom of the sealing tube.

[0011] Preferably, a vertical mounting plate is fixedly connected to the top of the inner surface of the communicating pipe. An elastic ejector rod is fixedly connected to the side surface of the vertical mounting plate, and the other end of the elastic ejector rod is fixedly connected to a conical sealing plate.

[0012] Preferably, the inner part of one end of the communicating pipe connected to the feeding pipe is a conical surface, and the conical sealing plate abuts against the conical surface.

[0013] (III) Beneficial effects Compared with the prior art, the present invention provides a strain preservation tube and a strain preservation tube cap, having the following beneficial effects: 1. For the strain preservation tube and the strain preservation tube cap, through the cooperative setting of the adding tube, the pressing rod, the sealing plug, the one-way feeding mechanism, the feeding pipe and the communicating pipe, during the use process, it can ensure that the inside of the preservation tube body is always isolated from the external environment during the process of adding a protective agent or other reagents, and only the reagent can enter the inside of the preservation tube body, avoiding the entry of external miscellaneous bacteria into the preservation tube body to contaminate the internally cultured strains during the reagent adding process.

[0014] 2. For the strain preservation tube and the strain preservation tube cap, through the cooperative setting of the reset tube, the pressure relief tube, the exhaust valve, the air outlet hole and the side pull plate, during the use process, it can balance the air pressure in the preservation tube body after adding the reagent, avoiding the increase of the air pressure in the preservation tube body from affecting the normal cultivation of the strains. Through the cooperative setting of the preservation tube body and the preservation tube bottom, during the use process, when cleaning is required, the preservation tube bottom can be disassembled for cleaning, which is more convenient for reuse. BRIEF DESCRIPTION OF THE DRAWINGS

[0015] Figure 1 is a schematic structural diagram of the whole of the present invention; Figure 2 is a schematic structural diagram of the front view cross-section of the bottom of the preservation tube body of the present invention; Figure 3 is a schematic structural diagram of the front view cross-section of the reset tube of the present invention; Figure 4 is a schematic structural diagram of the front view cross-section of the top of the preservation tube body of the present invention; Figure 5 is a schematic structural diagram of the cross-section at A of the present invention.

[0016] In the figure: 1. Preservation tube body; 101. Thread connection groove; 102. Bottom sealing ring; 2. Preservation tube bottom; 201. Thread connection ring; 3. Reset tube; 301. Reset spring; 4. Pressure relief tube; 401. Reset plate; 402. Sealing gasket; 5. Exhaust valve; 6. Air outlet hole; 7. Side pull plate; 8. Tube cap; 9. Adding tube; 901. Stop ring; 10. Pressing rod; 11. Sealing plug; 12. Unidirectional feeding mechanism; 1201. Sealing tube; 1202. Installation ring; 1203. Tensile spring; 1204. Feeding plug; 13. Feeding tube; 14. Connecting tube; 1401. Vertical installation plate; 1402. Elastic ejector rod; 1403. Conical plugging plate. Specific implementation mode

[0017] Next, the technical solutions in the embodiments of the present invention will be clearly and completely described in conjunction with the accompanying drawings in the embodiments of the present invention. Obviously, the described embodiments are only a part of the embodiments of the present invention, rather than all the embodiments. All other embodiments obtained by those of ordinary skill in the art based on the embodiments of the present invention without creative efforts shall fall within the protection scope of the present invention.

[0018] Please refer to Figures 1-5, a strain preservation tube and its tube cap, including a preservation tube body 1, a preservation tube bottom 2 is detachably connected to the bottom of the preservation tube body 1. A threaded connection groove 101 is provided at the bottom of the preservation tube body 1. A bottom sealing ring 102 is fixedly connected to the top of the inner surface of the threaded connection groove 101. A threaded connection ring 201 is fixedly connected to the upper surface of the preservation tube bottom 2. The thread on the threaded connection ring 201 is adapted to the thread in the threaded connection groove 101, and the top of the threaded connection ring 201 abuts against the bottom of the bottom sealing ring 102; A reset tube 3 is fixedly connected to the side of the preservation tube body 1. A pressure relief tube 4 is inserted into the reset tube 3. A reset spring 301 is sleeved on the outer surface of a section of the pressure relief tube 4 inserted into the reset tube 3. A reset plate 401 is fixedly connected to the outer surface of the pressure relief tube 4. One end of the reset spring 301 abuts against the side of the reset plate 401, and the other end abuts against the outer surface of the preservation tube body 1. An exhaust valve 5 is provided at one end of the pressure relief tube 4 outside the reset tube 3. The exhaust valve 5 is a one-way valve, and the gas flow direction in the exhaust valve 5 is from the inside of the preservation tube body 1 to the outside. A sealing pad 402 is fixedly connected to one end of the pressure relief tube 4 inserted into the preservation tube body 1. The sealing pad 402 abuts against the side wall of the inner surface of the preservation tube body 1. An air outlet hole 6 is provided at one end of the pressure relief tube 4 inserted into the reset tube 3. A side pull plate 7 is provided on the outer surface of the pressure relief tube 4 between the exhaust valve 5 and the reset tube 3; A tube cap 8 is detachably connected to the top of the preservation tube body 1. A filling tube 9 is fixedly connected to the center position of the upper surface of the tube cap 8. A retaining ring 901 is fixedly connected to the side wall of the inner surface of the filling tube 9. The position of the retaining ring 901 is between the sealing plug 11 and the connecting tube 14. A pressing rod 10 is inserted into the filling tube 9. A sealing plug 11 is fixedly connected to one end of the pressing rod 10 inserted into the filling tube 9. An opening is provided on the tube cap 8 corresponding to the filling tube 9. A one-way feeding mechanism 12 is provided on the lower surface of the tube cap 8 corresponding to the filling tube 9. The one-way feeding mechanism 12 includes a sealing tube 1201. An installation ring 1202 is fixedly connected to the side wall of the inner surface of the sealing tube 1201. A tension spring 1203 is fixedly connected to the bottom of the installation ring 1202. A feeding plug 1204 is fixedly connected to the bottom of the tension spring 1203. The top of the feeding plug 1204 abuts against the bottom of the sealing tube 1201. A feeding tube 13 is provided on the upper surface of the tube cap 8 on one side of the filling tube 9. The feeding tube 13 is connected to the filling tube 9 through a connecting tube 14. A vertical installation plate 1401 is fixedly connected to the top of the inner surface of the connecting tube 14. An elastic ejector rod 1402 is fixedly connected to the side of the vertical installation plate 1401. The other end of the elastic ejector rod 1402 is fixedly connected to a conical plugging plate 1403. The inner surface of the connecting tube 14 at the end connected to the feeding tube 13 is a conical surface. The conical plugging plate 1403 abuts against the conical surface.

[0019] During use, when a reagent needs to be added, first clean the inside of the feeding tube 13 exposed to the outside. Then add the reagent into the feeding tube 13. Subsequently, pull up the pressing rod 10 to form a negative pressure inside the adding tube 9 and the connecting tube 14. Under the action of the negative pressure, the conical sealing plate 1403 compresses the elastic ejector rod 1402 to open the end of the connecting tube 14 communicating with the feeding tube 13, enabling the reagent to enter the adding tube 9. Then press the pressing rod 10 downwards. Under the action of air pressure, the feeding plug 1204 pulls the tension spring 1203 downwards to open the bottom of the sealing tube 1201, allowing the reagent to enter the inside of the storage tube body 1. During the entire adding process, the storage tube body 1 is always not connected to the external environment, providing effective protection. Subsequently, push the pressure relief tube 4 into the storage tube body 1 through the side pulling plate 7, so that the air outlet hole 6 on the pressure relief tube 4 enters the storage tube body 1. Then, under the action of air pressure, the excess gas is discharged outwards to ensure that the air pressure inside the storage tube body 1 is the same as that of the outside. After releasing the side pulling plate 7, the pressure relief tube 4 resets under the action of the return spring 301. After resetting, the sealing gasket 402 abuts against the side wall of the inner surface of the storage tube body 1 to form a seal again.

[0020] In summary, for this strain storage tube and its strain storage tube cap, through the cooperative setting of the adding tube 9, the pressing rod 10, the sealing plug 11, the one-way feeding mechanism 12, the feeding tube 13 and the connecting tube 14, during use, it can ensure that the inside of the storage tube body 1 is always isolated from the external environment during the process of adding a protective agent or other reagents, and only the reagent can enter the inside of the storage tube body 1, preventing external miscellaneous bacteria from entering the storage tube body 1 and contaminating the internally cultured strains during the reagent addition process; through the cooperative setting of the return tube 3, the pressure relief tube 4, the exhaust valve 5, the air outlet hole 6 and the side pulling plate 7, during use, it can balance the air pressure of the storage tube body 1 after adding the reagent, preventing the increase in air pressure inside the storage tube body 1 from affecting the normal cultivation of the strains. Through the cooperative setting of the storage tube body 1 and the storage tube bottom 2, during use, when cleaning is required, the storage tube bottom 2 can be disassembled for cleaning, which is more convenient for reuse.

[0021] It should be noted that in this text, relational terms such as first and second are only used to distinguish one entity or operation from another entity or operation, and do not necessarily require or imply any actual relationship or order between these entities or operations. Moreover, the term "comprising", "including" or any other variant thereof is intended to cover non-exclusive inclusion, so that a process, method, article or device comprising a series of elements not only includes those elements, but also includes other elements not expressly listed, or also includes elements inherent in such process, method, article or device. Without further limitation, an element defined by the statement "comprising an" does not exclude the presence of additional identical elements in the process, method, article or device comprising said element.

[0022] Although the embodiments of the present invention have been shown and described, it will be understood by those of ordinary skill in the art that various changes, modifications, substitutions and variations can be made to these embodiments without departing from the principles and spirit of the present invention, and the scope of the present invention is defined by the appended claims and their equivalents.

Claims

1. A strain preservation tube and a strain preservation tube cover thereof, comprising a preservation tube body (1), characterized in that: The bottom of the storage tube body (1) is detachably connected to the storage tube bottom (2); the side of the storage tube body (1) is fixedly connected to the reset tube (3); the reset tube (3) is plugged with a pressure relief tube (4) inside the reset tube (3); an exhaust valve (5) is provided at one end of the pressure relief tube (4) located outside the reset tube (3); an exhaust hole (6) is provided at one end of the pressure relief tube (4) plugged into the reset tube (3); a side pull plate (7) is provided on the outer surface of the pressure relief tube (4) between the exhaust valve (5) and the reset tube (3); a tube cover (8) is detachably connected to the top of the storage tube body (1); the tube cover ( An addition pipe (9) is fixedly connected to the center position of the upper surface of the pipe cover (8), a lower pressure rod (10) is inserted into the interior of the addition pipe (9), and a sealing plug (11) is fixedly connected to one end of the lower pressure rod (10) inserted into the addition pipe (9). An opening is provided at a position corresponding to the addition pipe (9) on the pipe cover (8), a one-way feeding mechanism (12) is provided at a position corresponding to the addition pipe (9) on the lower surface of the pipe cover (8), and a addition pipe (13) is provided on one side of the addition pipe (9) on the upper surface of the pipe cover (8), and the addition pipe (13) is connected to the addition pipe (9) via a connecting pipe (14).

2. A strain storage tube and strain storage tube cover according to claim 1, characterized in that: A threaded connection groove (101) is provided at the bottom of the storage tube body (1), and a bottom sealing ring (102) is fixedly connected to the top of the inner surface of the threaded connection groove (101).

3. A strain storage tube and strain storage tube cover according to claim 2, characterized in that: A threaded connection ring (201) is fixedly connected to the upper surface of the storage tube bottom (2), the threads on the threaded connection ring (201) are adapted to the threads in the threaded connection groove (101), and the top of the threaded connection ring (201) abuts against the bottom of the bottom sealing ring (102).

4. The bacterial culture storage tube and the bacterial culture storage tube cover according to claim 1, characterized in that: A return spring (301) is sleeved on the outer surface of a section of the pressure relief pipe (4) inserted into the return pipe (3); a return plate (401) is fixedly connected to the outer surface of the pressure relief pipe (4); one end of the return spring (301) abuts against the side surface of the return plate (401), and the other end abuts against the outer surface of the storage pipe body (1).

5. The bacterial culture storage tube and the bacterial culture storage tube cover according to claim 1, characterized in that: The exhaust valve (5) is a one-way valve, and the gas flow direction in the exhaust valve (5) is from the inside of the storage tube body (1) to the outside. One end of the pressure relief tube (4) inserted into the storage tube body (1) is fixedly connected to a sealing gasket (402), and the sealing gasket (402) abuts against the side wall of the inner surface of the storage tube body (1).

6. The bacterial culture storage tube and the bacterial culture storage tube cover according to claim 1, characterized in that: A retaining ring (901) is fixedly connected to the side wall of the inner surface of the adding pipe (9), and the retaining ring (901) is arranged between the sealing plug (11) and the connecting pipe (14).

7. The bacterial culture storage tube and the bacterial culture storage tube cover according to claim 1, characterized in that: The one-way feeding mechanism (12) comprises a sealing tube (1201), a mounting ring (1202) being fixedly connected to the side wall of the inner surface of the sealing tube (1201), a tension spring (1203) being fixedly connected to the bottom of the mounting ring (1202), a feeding plug (1204) being fixedly connected to the bottom of the tension spring (1203), and the top of the feeding plug (1204) abutting against the bottom of the sealing tube (1201).

8. The bacterial culture storage tube and the bacterial culture storage tube cover according to claim 1, characterized in that: A vertical mounting plate (1401) is fixedly connected to the top of the inner surface of the connecting pipe (14), an elastic top rod (1402) is fixedly connected to the side of the vertical mounting plate (1401), and a conical blocking plate (1403) is fixedly connected to the other end of the elastic top rod (1402).

9. A bacterial culture storage tube and a bacterial culture storage tube cover according to claim 8, characterized in that: The interior of one end of the connecting pipe (14) connected to the feeding pipe (13) is a conical surface, and the conical sealing plate (1403) abuts against the conical surface.

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