Device for improving in-vitro development efficiency of nuclear transfer embryo

Abstract

The invention relates to the technical field of nuclear transplantation, in particular to a device for improving the in-vitro development efficiency of a nuclear transplantation embryo, the device comprises a box body and an electric push rod, the electric push rod is fixedly connected to the top wall of the box body, a sterilization mechanism is arranged in the box body, and the sterilization mechanism comprises a storage cylinder, a rotating rod, a stirring rod, a magnetic block and a copper sheet. The storage cylinder is fixedly connected to the bottom wall of the box body, the rotating rod is rotatably connected to the top wall of the storage cylinder, and the stirring rods are installed on the outer surface of the rotating rod in an annular array mode. A nutrient solution can be regularly injected only by operating the electric push rod externally, the whole process of karyocyte development is in a constant environment suitable for karyocyte development, the culture dish does not need to be taken out, infection caused by contact between the culture dish and the outside is avoided, and the efficiency of in-vitro development of nuclear transfer embryos is guaranteed.

Description

Technical Field

[0001] The present invention relates to the technical field of nuclear transfer, and particularly relates to a device for improving the in vitro development efficiency of nuclear transfer embryos. Background Art

[0002] Nuclear transfer is a method of transplanting a donor cell nucleus into an enucleated oocyte, enabling the latter to be activated, divided, and developed without sexual processes such as sperm penetration, completely replicating the genes of the nuclear donor. After culturing for a period of time, the developing oocyte is then transplanted into a human or animal body.

[0003] During the process of nuclear transfer, the culture dish is regularly taken out of the incubator to add corresponding nutrients for cell development. In this process, the culture dish is prone to contact with the external environment, thereby being infected with bacteria, resulting in culture failure and reducing the in vitro development efficiency of nuclear transfer embryos. Summary of the Invention

[0004] The purpose of the present invention is to solve the following drawbacks in the prior art. During the process of nuclear transfer, the culture dish is regularly taken out of the incubator to add corresponding nutrients for cell development. In this process, the culture dish is prone to contact with the external environment, thereby being infected with bacteria, resulting in culture failure and reducing the in vitro development efficiency of nuclear transfer embryos. Therefore, a device for improving the in vitro development efficiency of nuclear transfer embryos is proposed.

[0005] To achieve the above purpose, the present invention adopts the following technical scheme:

[0006] A device for improving the in vitro development efficiency of nuclear transfer embryos includes a box body and an electric push rod, and the electric push rod is fixedly connected to the top wall of the box body;

[0007] A sterilization mechanism is provided inside the box body. The sterilization mechanism includes a storage cylinder, a rotating rod, a stirring rod, a magnetic block, and a copper sheet. The storage cylinder is fixedly connected to the bottom wall of the box body, the rotating rod is rotatably connected to the top wall of the storage cylinder, the stirring rod is installed on the outer surface of the rotating rod in an annular array, and a plurality of magnetic blocks are respectively fixedly connected to one end of the stirring rod away from the rotating rod. An installation groove is opened on the inner side wall of the storage cylinder, and the copper sheet is fixedly connected in the installation groove;

[0008] An adjustment mechanism is also provided inside the box body. The adjustment mechanism includes a driving rod, a ratchet wheel, a ratchet pawl, and a belt. The driving rod is rotatably connected to the upper end surface of the storage cylinder, the ratchet wheel is fixedly connected to the outer surface of the driving rod, the ratchet pawl is rotatably connected to the upper end surface of the storage cylinder, the ratchet pawl is slidably connected to the ratchet wheel, and the belt is sleeved between the driving rod and the rotating rod.

[0009] Preferably, the upper end surface of the storage cylinder is fixedly and rotatably connected with an adjusting rod, an adjusting disc is fixedly connected to the outer surface of the adjusting rod, a friction disc is fixedly connected to the outer surface of the driving rod, and the adjusting disc is slidably connected with the friction disc.

[0010] Preferably, a limiting block is fixedly connected to the side wall of the box body, a rack plate is slidably connected to the inner side wall of the limiting block, a gear is fixedly connected to the outer surface of the adjusting rod, and the rack plate is meshed with the gear.

[0011] Preferably, a fixing block is fixedly connected to the side wall of the storage cylinder, an air cylinder is fixedly connected to one end of the fixing block away from the storage cylinder, and a piston plate is slidably connected to the inner side wall of the air cylinder.

[0012] Preferably, an extrusion plate is fixedly connected to the upper end surface of the piston plate, the extrusion plate is slidably connected to one side wall of the air cylinder, a push plate is fixedly connected to one end of the extrusion plate away from the piston plate, and the push plate is fixedly connected to the telescopic end of the electric push rod.

[0013] Preferably, chutes are respectively formed at both ends of the push plate, lead screws are respectively rotatably connected to the inner side walls of the two chutes, connecting plates are respectively threadedly connected to the two lead screws, and the connecting plates are slidably connected to the inner side walls of the chutes.

[0014] Preferably, impact rods are fixedly connected to one ends of the two connecting plates close to the air cylinder, counterweights are respectively fixedly connected to one ends of the impact rods away from the connecting plates, C-shaped frames are linearly arranged at both ends of the air cylinder, and the impact rods are slidably connected to the C-shaped frames.

[0015] Preferably, a triangular block is fixedly connected to one end of the push plate close to the storage cylinder, a trapezoidal block is fixedly connected to one end of the rack plate close to the triangular block, and the trapezoidal block is slidably connected to the triangular block.

[0016] Preferably, a culture dish is arranged on the inner side wall of the box body, an air outlet pipe is fixedly communicated between the culture dish and the air cylinder, an air inlet pipe is fixedly communicated between the storage cylinder and the air cylinder, one-way valves are arranged in both the air inlet pipe and the air outlet pipe, a return spring is fixedly connected between the rack plate and the limiting block, and a torsion spring is fixedly connected between the ratchet and the storage cylinder.

[0017] Compared with the prior art, the beneficial effects of the present invention are:

[0018] 1. By placing the nutrient solution and the syringe in advance in a sterile incubator, it is only necessary to operate the electric push rod externally to regularly add the nutrient solution. The whole process of nuclear cell development is in a constant and suitable environment for its development, without taking out the culture dish, avoiding infection caused by contact with the outside world, and ensuring the efficiency of in vitro development of nuclear transfer embryos.

[0019] 2. By driving the triangular block to move through the push plate, squeezing the trapezoidal block, driving the rack plate to move, and cooperating with the ratchet and pawl, the magnetic block belt rotates after each injection of the nutrient solution into the culture dish, and eddy currents are induced in the copper sheet to generate heat, heating and sterilizing the nutrient solution, avoiding the growth of bacteria in the storage cylinder and thus infecting the culture dish, and ensuring the efficiency of in vitro development of nuclear transfer embryos.

[0020] 3. By driving the connecting plate to move through the push plate, when injecting the nutrient solution into the culture dish each time, the impact rod is driven to continuously impact the C-shaped frame, generating vibrations to break the surface tension of the nutrient solution, avoiding the nutrient solution adhering to the inner side wall of the syringe, avoiding the growth of bacteria in the syringe, and at the same time accurately controlling the amount of nutrient solution injected. There will be no nutrient solution remaining in the syringe to cause errors. At the same time, only by rotating the screw rod to change the distance between the connecting plate and the syringe, the vibration intensity is changed, and the most suitable intensity is selected according to the different viscosities of different nutrient solutions, ensuring the efficiency of in vitro development of nuclear transfer embryos. Brief Description of the Drawings

[0021] Figure 1 Schematic diagram of the culture dish structure of a device for improving the in vitro development efficiency of nuclear transfer embryos proposed by the present invention;

[0022] Figure 2 Schematic diagram of the fixed block structure of a device for improving the in vitro development efficiency of nuclear transfer embryos proposed by the present invention;

[0023] Figure 3 Schematic diagram of the piston plate structure of a device for improving the in vitro development efficiency of nuclear transfer embryos proposed by the present invention;

[0024] Figure 4 For Figure 3 Partial enlarged structure diagram of A in

[0025] Figure 5 Schematic diagram of the magnetic block structure of a device for improving the in vitro development efficiency of nuclear transfer embryos proposed by the present invention;

[0026] Figure 6 Schematic diagram of the trapezoidal block structure of a device for improving the in vitro development efficiency of nuclear transfer embryos proposed by the present invention;

[0027] Figure 7 For Figure 6 Partial enlarged structure diagram of B in

[0028] In the figure: 1 box body, 2 electric push rod, 3 storage cylinder, 4 rotating rod, 5 stirring rod, 6 magnetic block, 7 copper sheet, 8 driving rod, 9 ratchet wheel, 10 ratchet pawl, 11 belt, 12 adjusting rod, 13 adjusting disc, 14 friction disc, 15 limiting block, 16 rack plate, 17 gear, 18 fixing block, 19 air cylinder, 20 piston plate, 21 extrusion plate, 22 push plate, 23 lead screw, 24 connecting plate, 25 impact rod, 26 counterweight, 27 C-shaped frame, 28 triangular block, 29 trapezoidal block, 30 culture dish, 31 air outlet pipe, 32 air inlet pipe, 33 return spring, 34 torsion spring. Specific embodiments

[0029] Next, the technical solutions in the embodiments of the present invention will be clearly and completely described in conjunction with the accompanying drawings in the embodiments of the present invention. Obviously, the described embodiments are only a part of the embodiments of the present invention, rather than all the embodiments.

[0030] Referring to Figures 1-7 , a device for improving the in vitro development efficiency of nuclear transfer embryos, including a box body 1 (the box body 1 is an incubator, which can provide suitable temperature, oxygen content, etc. for the development of nuclear cells, and is the prior art and will not be elaborated here too much) and an electric push rod 2. The front surface of the box body 1 is made of a transparent material so that the internal situation can be observed, and the electric push rod 2 is fixedly connected to the top wall of the box body 1.

[0031] A sterilization mechanism is provided inside the box body 1. The sterilization mechanism includes a storage cylinder 3, a rotating rod 4, a stirring rod 5, a magnetic block 6, and a copper sheet 7. The storage cylinder 3 is fixedly connected to the bottom wall of the box body 1. The storage cylinder 3 stores the nutrient solution required for the development of nuclear cells. The outer wall of the storage cylinder 3 is made of a heat-insulating material, but its heat-insulating performance is relatively general, ensuring that the heat inside it can be dissipated smoothly. The rotating rod 4 is hermetically and rotatably connected to the top wall of the storage cylinder 3. The stirring rod 5 is arranged in an annular array on the outer surface of the rotating rod 4. A plurality of magnetic blocks 6 are respectively fixedly connected to one end of the stirring rod 5 far from the rotating rod 4. Some coatings are wrapped on the outer surface of the magnetic block 6 to prevent the magnetic block 6 from directly reacting with the nutrient solution. An installation groove is opened on the inner side wall of the storage cylinder 3, and the copper sheet 7 is fixedly connected in the installation groove. An adjusting mechanism is also provided inside the box body 1. The adjusting mechanism includes a driving rod 8, a ratchet wheel 9, a ratchet pawl 10, and a belt 11. The driving rod 8 is rotatably connected to the upper end surface of the storage cylinder 3. The ratchet wheel 9 is fixedly connected to the outer surface of the driving rod 8. The ratchet pawl 10 is rotatably connected to the upper end surface of the storage cylinder 3. A torsion spring 34 is fixedly connected between the ratchet pawl 10 and the storage cylinder 3. The ratchet pawl 10 is slidably connected to the ratchet wheel 9. The belt 11 is sleeved between the driving rod 8 and the rotating rod 4.

[0032] The upper end face of the storage cylinder 3 is fixedly and rotatably connected with an adjusting rod 12. The outer surface of the adjusting rod 12 is fixedly connected with an adjusting disc 13. The diameter of the adjusting disc 13 is much larger than that of the friction disc 14, that is, when the adjusting rod 12 rotates one circle, the friction disc 14 can be rotated multiple circles through the adjusting disc 13. Both the friction disc 14 and the adjusting disc 13 are very wear-resistant. The outer surface of the driving rod 8 is fixedly connected with a friction disc 14, and the adjusting disc 13 is slidably connected with the friction disc 14.

[0033] A limiting block 15 is fixedly connected to the side wall of the box body 1. A rack plate 16 is slidably connected to the inner side wall of the limiting block 15. The rack plate 16 only has partial teeth. The part of the rack plate 16 connected to the limiting block 15 is relatively smooth. A return spring 33 is fixedly connected between the rack plate 16 and the limiting block 15. The outer surface of the adjusting rod 12 is fixedly connected with a gear 17, and the rack plate 16 is meshed with the gear 17.

[0034] A fixing block 18 is fixedly connected to the side wall of the storage cylinder 3. One end of the fixing block 18 far away from the storage cylinder 3 is fixedly connected with an air cylinder 19. A piston plate 20 is hermetically and slidably connected to the inner side wall of the air cylinder 19. The upper end face of the piston plate 20 is fixedly connected with a pressing plate 21. The pressing plate 21 is slidably connected to one side wall of the air cylinder 19. The pressing plate 21 is not sealed with the air cylinder 19. One end of the pressing plate 21 far away from the piston plate 20 is fixedly connected with a push plate 22. The push plate 22 is fixedly connected to the telescopic end of the electric push rod 2.

[0035] Chute grooves are respectively formed at both ends of the push plate 22. The inner side walls of the two chute grooves are respectively rotatably connected with a lead screw 23. A connecting plate 24 is respectively threadedly connected to the two lead screws 23. The connecting plate 24 is slidably connected to the inner side wall of the chute groove. One ends of the two connecting plates 24 close to the air cylinder 19 are both fixedly connected with an impact rod 25. The impact rod 25 has good elasticity and will bend and deform when subjected to a certain external force and will quickly restore its own shape after the external force disappears. One ends of the impact rods 25 far away from the connecting plates 24 are respectively fixedly connected with a counterweight 26. C-shaped frames 27 are respectively installed at both ends of the air cylinder 19 in a linear array. The impact rods 25 are slidably connected with the C-shaped frames 27. One end of the push plate 22 close to the storage cylinder 3 is fixedly connected with a triangular block 28. One end of the rack plate 16 close to the triangular block 28 is fixedly connected with a trapezoidal block 29. The trapezoidal block 29 is slidably connected with the triangular block 28.

[0036] On the inner side wall of the box body 1, there is a culture dish 30. A gas outlet pipe 31 is fixedly connected between the culture dish 30 and the air cylinder 19. The culture dish 30 is divided into a cover body and a main body. The gas outlet pipe 31 is fixedly connected to the cover body. The cover body can be removed to transplant the nuclear cells into the main body of the culture dish 30. Closing the cover body can seal the culture dish 30. A gas inlet pipe 32 is fixedly connected between the storage cylinder 3 and the air cylinder 19. One-way valves are provided in both the gas inlet pipe 32 and the gas outlet pipe 31. The flow direction of the one-way valve in the gas inlet pipe 32 is from the storage cylinder 3 to the air cylinder 19, and the flow direction of the one-way valve in the gas outlet pipe 31 is from the air cylinder 19 to the culture dish 30.

[0037] In the present invention, when in use, first disinfect all the equipment in the box body 1, then open the culture dish 30, inject the culture solution containing nuclear cells into the culture dish 30, close the lid of the culture dish 30 to seal it, and close the door of the box body 1 for cultivation. When it is necessary to inject nutrient solution into the culture dish 30 at intervals, only start the electric push rod 2 to contract. The contraction of the electric push rod 2 drives the push plate 22 to move upward. The upward movement of the push plate 22 drives the piston plate 20 to move upward. The air cylinder 19 is in a negative pressure state. The one-way valve in the gas inlet pipe 32 opens, and the one-way valve in the gas outlet pipe 31 closes. The nutrient solution in the storage cylinder 3 enters the air cylinder 19 along the gas inlet pipe 32. The upward movement of the push plate 22 drives the triangular block 28 to move upward. The upward movement of the triangular block 28 squeezes the trapezoidal block 29. The trapezoidal block 29 is squeezed and drives the rack plate 16 to move towards the limit block 15, and compresses the return spring 33. The rack plate 16 drives the gear 17 and the adjusting rod 12 to rotate. The adjusting rod 12 drives the adjusting disk 13 to rotate. The adjusting disk 13 drives the friction disk 14 and the driving rod 8 to rotate clockwise. The driving rod 8 drives the ratchet 9 to rotate clockwise. The clockwise rotation of the ratchet 9 is restricted by the pawl 10 and cannot rotate, so the driving rod 8 does not move.

[0038] Then, the electric push rod 2 starts to expand. The expansion of the electric push rod 2 drives the push plate 22 to move downward. The downward movement of the push plate 22 drives the piston plate 20 to move downward. The inside of the air cylinder 19 is under positive pressure. The one-way valve in the air inlet pipe 32 closes, and the one-way valve in the air outlet pipe 31 opens. The nutrient solution in the air cylinder 19 is injected into the culture dish 30 along the air outlet pipe 31. The downward movement of the push plate 22 drives the triangular block 28 to move downward. The squeezing force of the triangular block 28 on the trapezoidal block 29 disappears. Under the elastic force of the return spring 33, the trapezoidal block 29 drives the rack plate 16 to move away from the limit block 15. The rack plate 16 drives the gear 17 and the adjusting rod 12 to rotate. The adjusting rod 12 drives the adjusting disk 13 to rotate. The adjusting disk 13 drives the friction disk 14 and the driving rod 8 to rotate counterclockwise. The driving rod 8 drives the ratchet 9 to rotate counterclockwise. The counterclockwise rotation of the ratchet 9 is not limited by the pawl 10. The driving rod 8 drives the rotating rod 4 to rotate at high speed through the belt 11. The rotating rod 4 drives the stirring rod 5 and the magnetic block 6 to rotate at high speed, inducing eddy current heating in the copper sheet 7. The heat is transferred to the nutrient solution to heat it and kill the internal bacteria. The outer wall of the storage cylinder 3 is made of heat-insulating material, but its heat-insulating performance is relatively general, ensuring that the heat inside it can be dissipated relatively smoothly. Under the constant temperature effect of the box body 1, the heat of the storage cylinder 3 is slowly dissipated. During the interval between injecting the nutrient solution, the nutrient solution in the heated storage cylinder 3 generally takes several hours to dozens of hours to slowly cool down to the same temperature as that in the box body 1, without changing the temperature in the box body 1 and avoiding affecting the development of the nuclear cells in the culture dish 30.

[0039] During the upward and downward movement of the push plate 22, it drives the connecting plate 24 and the impact rod 25 to move. When the impact rod 25 contacts the C-shaped frame 27, it will bend and deform, and then, under the action of the inertia of the counterweight 26, it hits a C-shaped frame 27 located behind, continuously hitting to generate vibration, breaking the surface tension of the nutrient solution, preventing a small amount of nutrient solution from adhering to the inner wall of the air cylinder 19 and breeding bacteria, and ensuring the accuracy of the single injection amount of the nutrient solution. Rotating the screw rod 23 drives the connecting plate 24 to move, making the connecting plate 24 approach or move away from the air cylinder 19, that is, changing the distance between the impact rod 25 and the C-shaped frame 27, that is, changing the degree of deformation of the impact rod 25, that is, changing the intensity of the vibration, and adjusting the vibration force to an appropriate size according to the viscosity of different nutrient solutions.

[0040] Regularly injecting the nutrient solution only needs to be operated outside the box body 1, without opening the box body 1 to take out the culture dish 30, avoiding its contact with the external environment and infection, and ensuring the efficiency of the in vitro development of the nuclear transfer embryos.

[0041] The above is only the preferred specific embodiment of the present invention, but the protection scope of the present invention is not limited thereto. Any person skilled in the art within the technical scope disclosed by the present invention, according to the technical solution and inventive concept of the present invention, making equivalent substitutions or changes, should be covered by the protection scope of the present invention.

Claims

1. A device for improving the efficiency of in vitro development of nuclear transplanted embryos, comprising a housing (1) and an electric push rod (2), characterized in that: The electric push rod (2) is fixedly connected to the top wall of the box (1); The box body (1) is provided with a sterilization mechanism, and the sterilization mechanism comprises a storage cylinder (3), a rotating rod (4), a stirring rod (5), a magnetic block (6), and a copper sheet (7). The storage cylinder (3) is fixedly connected to the bottom wall of the box body (1), the rotating rod (4) is rotatably connected to the top wall of the storage cylinder (3), the stirring rod (5) is installed in a circular array on the outer surface of the rotating rod (4), a plurality of magnetic blocks (6) are respectively fixedly connected to one end of the stirring rod (5) away from the rotating rod (4), an installation groove is opened on the inner side wall of the storage cylinder (3), and the copper sheet (7) is fixedly connected in the installation groove; The box body (1) is also provided with an adjustment mechanism, which includes a driving rod (8), a ratchet wheel (9), a pawl (10), and a belt (11). The driving rod (8) is rotatably connected to the upper end surface of the storage tube (3), the ratchet wheel (9) is fixedly connected to the outer surface of the driving rod (8), the pawl (10) is rotatably connected to the upper end surface of the storage tube (3), the pawl (10) is slidably connected to the ratchet wheel (9), and the belt (11) is sleeved between the driving rod (8) and the rotating rod (4).

2. A device for improving the efficiency of in vitro development of nuclear transplanted embryos according to claim 1, characterized in that: The upper end surface of the storage cylinder (3) is fixedly rotatably connected to an adjusting rod (12), the outer surface of the adjusting rod (12) is fixedly connected to an adjusting disk (13), the outer surface of the driving rod (8) is fixedly connected to a friction disk (14), and the adjusting disk (13) is slidably connected to the friction disk (14).

3. The device for improving the efficiency of in vitro development of nuclear transplanted embryos according to claim 2, characterized in that: A limit block (15) is fixedly connected to the side wall of the box body (1), a rack plate (16) is slidably connected to the inner wall of the limit block (15), a gear (17) is fixedly connected to the outer surface of the adjustment rod (12), and the rack plate (16) is meshingly connected to the gear (17).

4. The device for improving the efficiency of in vitro development of nuclear transplanted embryos according to claim 3, characterized in that: A fixing block (18) is fixedly connected to the side wall of the storage cylinder (3), an end of the fixing block (18) away from the storage cylinder (3) is fixedly connected to a gas cylinder (19), and a piston plate (20) is slidably connected to the inner side wall of the gas cylinder (19).

5. The device for improving the efficiency of in vitro development of nuclear transplanted embryos according to claim 4, characterized in that: The upper end surface of the piston plate (20) is fixedly connected to an extrusion plate (21), and the extrusion plate (21) is slidably connected to one side wall of the air cylinder (19). The end of the extrusion plate (21) away from the piston plate (20) is fixedly connected to a push plate (22), and the push plate (22) is fixedly connected to the telescopic end of the electric push rod (2).

6. The device for improving the efficiency of in vitro development of nuclear transplanted embryos according to claim 5, characterized in that: The two ends of the push plate (22) are respectively provided with a slide groove, the inner side walls of the two slide grooves are respectively rotatably connected with a screw rod (23), the two screw rods (23) are respectively threadedly connected with a connecting plate (24), and the connecting plate (24) is slidably connected to the inner side wall of the slide groove.

7. The device for improving the efficiency of in vitro development of nuclear transplanted embryos according to claim 6, characterized in that: The two connecting plates (24) are fixedly connected to one end close to the air cylinder (19) with a striking rod (25), and the ends of the striking rods (25) away from the connecting plates (24) are respectively fixedly connected to a counterweight head (26). Both ends of the air cylinder (19) are respectively installed with C-shaped frames (27) in a linear array, and the striking rods (25) are slidably connected to the C-shaped frames (27).

8. The device for improving the efficiency of in vitro development of nuclear transplanted embryos according to claim 5, characterized in that: The end of the push plate (22) close to the storage cylinder (3) is fixedly connected to a triangular block (28), and the end of the rack plate (16) close to the triangular block (28) is fixedly connected to a trapezoidal block (29), and the trapezoidal block (29) is slidably connected to the triangular block (28).

9. The device for improving the efficiency of in vitro development of nuclear transplanted embryos according to claim 4, characterized in that: A culture dish (30) is provided on the inner side wall of the box body (1), an air outlet pipe (31) is fixedly connected between the culture dish (30) and the air cylinder (19), an air inlet pipe (32) is fixedly connected between the storage cylinder (3) and the air cylinder (19), and a one-way valve is provided in each of the air inlet pipe (32) and the air outlet pipe (31), a return spring (33) is fixedly connected between the rack plate (16) and the limit block (15), and a torsion spring (34) is fixedly connected between the ratchet (10) and the storage cylinder (3).

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