Method and device for detecting effective components of liquid beverage capable of dredging meridians and collaterals and improving microcirculation

Abstract

The invention discloses a method and a device for detecting effective components of a liquid beverage capable of dredging meridians and collaterals and improving microcirculation, and relates to the technical field of liquid beverage detection. The detection method comprises the following steps: S01, obtaining a liquid beverage sample, and separating a solid phase and a liquid phase in the liquid beverage sample by adopting a circulating filtration method. Through a cross-phase state component tracing method, the matching degree A between a control gas and initial liquid phase effective component data and the matching degree B between the control gas and initial solid phase effective component data are analyzed, and then the matching degree A and the matching degree B are corrected through a matching calculation method and a source calculation method. Therefore, traceability and division of the effective components among different phase states are realized, division of the content of the effective components in the contrast gas is realized through a cross-phase-state component traceability method, and on the basis of obtaining the content of the effective components in the liquid beverage, the distribution condition of the content of the effective components is further obtained.

Description

Technical Field

[0001] The present invention relates to the technical field of liquid beverage detection, and specifically to a method and device for detecting the active ingredients of a liquid beverage that dredges meridians and improves microcirculation. Background Art

[0002] Liquid beverages may contain solids. Therefore, it is necessary not only to detect the components in the liquid, but also to be able to detect solids. For example, high performance liquid chromatography (HPLC), gas chromatography (GC), etc. These methods can separate and analyze the chemical components in beverages.

[0003] For example, the patent publication number "CN109507145A", titled "A Method for Detecting the Content of Industrial Liquid Thiourea by Near-Infrared", collects the near-infrared original spectra of samples on the basis of knowing the true content of a large number of samples, and establishes a quantitative analysis and prediction model for the content of thiourea in liquid thiourea feedstock based on near-infrared spectroscopy and liquid chromatography methods. During detection, only the sample to be tested needs to be pretreated and filtered, and then its near-infrared spectrogram is collected. After collecting the infrared spectrum, detection can be carried out, achieving the characteristics of short time for spectral collection, simplicity, quickness, high detection efficiency, and high detection accuracy, and is suitable for application in the production and use of industrial thiourea.

[0004] In the above patent, the infrared spectrum for single-phase detection is used in filtration. The single-phase detection method can detect in a single liquid phase, but after filtration, there may be active ingredients in the solid phase and liquid phase. Therefore, only detecting the single phase will cause deviation in the detection results.

[0005] For example, the patent publication number "CN112730687A", titled "A Method for Simultaneously Determining the Contents of Lignans and Terpenoids in Aucklandia and Saussurea Pill", uses a technology combining matrix solid-phase dispersion and extraction with a disposable pipette tip to extract the active ingredients in the traditional Chinese medicine Aucklandia and Saussurea Pill, integrating extraction and separation. Moreover, the amounts of samples, adsorbents, and elution solvents used are extremely small, having the advantages of low experimental cost, simple and convenient operation, short extraction and separation time, and high extraction efficiency. In the above patent, the liquid phase absorbs the active ingredients in the solid phase, but after the active ingredients are absorbed by the liquid phase, there are still active ingredients stored in the solid phase, resulting in the detected content of the active ingredients being less than the actual value. Therefore, a method and device for detecting the active ingredients of a liquid beverage that dredges meridians and improves microcirculation are invented. Summary of the Invention

[0006] The purpose of the present invention is to provide a method and device for detecting the active ingredients of a liquid beverage that dredges meridians and improves microcirculation, so as to solve the problems raised in the above background art.

[0007] To achieve the above object, the present invention provides the following technical solutions: A method and device for detecting the active ingredients of a liquid beverage for dredging meridians and improving microcirculation, and a method for detecting the active ingredients of a liquid beverage for dredging meridians and improving microcirculation, the detection method comprising: S01, obtaining a liquid beverage sample, separating the solid phase and the liquid phase in the liquid beverage sample by a circulation filtration method, applying orthogonal ultrasonic waves to the liquid beverage during the separation process, separately collecting the separated liquid phase and solid phase, detecting the separated liquid phase, and obtaining initial liquid phase active ingredient data; S02, performing a drying operation on the separated solid phase, obtaining a control gas discharged during the drying operation, homogenizing the dried solid phase, detecting and obtaining initial solid phase active ingredient data; S03, processing the control gas by a cross-phase state ingredient tracing method to obtain source data of the control gas, correcting the initial solid phase active ingredient data and the initial liquid phase active ingredient data according to the source data, and combining the corresponding gas phase content data with the initial liquid phase active ingredient data and the initial solid phase active ingredient data respectively according to the source data to obtain solid phase active ingredient data and liquid phase active ingredient data, and obtaining active ingredient distribution data based on the solid phase active ingredient data and the liquid phase active ingredient data; S04, jointly mapping the solid phase active ingredient data and the liquid phase active ingredient data on the liquid beverage to generate a distribution map and data of the active ingredients in the liquid beverage; The cross-phase state ingredient tracing method includes; Detecting the active ingredients of the control gas by a gas detection method to obtain control gas active ingredient data; Calculating the matching degree A between the control gas active ingredient data and the initial liquid phase active ingredient data and the matching degree B between the control gas active ingredient data and the initial solid phase active ingredient data by a matching calculation method, and calculating and obtaining the source data of the control gas by combining the matching degree A and the matching degree B by a source calculation method.

[0008] Furthermore, the matching calculation method includes: Dividing the active ingredients into volatile active ingredients X and non-volatile active ingredients Y according to the volatilization attributes of the active ingredients, and dividing them according to the quantity and volatilization attributes of the active ingredients to obtain Xi and Yj, where i represents the i-th volatile active ingredient and j represents the j-th non-volatile active ingredient, i (1, 2, 3... m), m represents the quantity of volatile active ingredients, j (1, 2, 3... n), n represents the quantity of non-volatile active ingredients; The matching degree A includes the matching degree AX between the volatile active ingredients X and the matching degree AY between the non-volatile active ingredients Y; The matching degree BA includes the matching degree BX between the volatile active ingredient X and the matching degree BY between the non-volatile active ingredient Y; obtain , , and , and respectively represent the content of the i-th volatile component and the content of the j-th non-volatile component in the initial liquid-phase active ingredient data, and respectively represent the content of the i-th volatile component and the content of the j-th non-volatile component in the control gas active ingredient data, and respectively represent the content of the i-th volatile component and the content of the j-th non-volatile component in the initial solid-phase active ingredient data, and respectively represent the matching degree of the i-th volatile component and the matching degree of the j-th non-volatile component in the matching degree A; The said source calculation method includes: Obtain source data based on the matching degree A and , and respectively represent the matching degree of the i-th volatile component and the matching degree of the j-th non-volatile component in the matching degree B. The source data includes , , and , and represent the division ratio between the liquid phase and the solid phase in the content of the i-th volatile component in the control gas, and represent the division ratio between the liquid phase and the solid phase in the content of the j-th volatile component in the control gas.

[0009] Furthermore, the said cross-phase component tracing method includes: Adopt the filtration membrane extraction method to obtain the second liquid from the separated liquid phase, perform partial vaporization operation on the second liquid to obtain the second gas, and detect the second gas by the gas detection method to obtain the second gas active ingredient data; Calculate the matching degree C between the second gas active ingredient data and the control gas active ingredient data through the matching calculation method, and calculate and obtain the source data of the control gas by combining the matching degree A, the matching degree B, and the matching degree C through the source calculation method.

[0010] 4. According to the active ingredient detection method of a liquid beverage for dredging meridians and improving microcirculation described in claim 3, furthermore, the said matching calculation method includes: The matching degree C includes the matching degree CX between the volatile active ingredients X and the matching degree CY between the non-volatile active ingredients Y; , and are the contents of the i-th volatile active ingredient in the control gas and in the second gas respectively, represents the matching degree of the i-th volatile active ingredient between the second gas and the control gas; The source calculation method includes: Based on correct the division ratio to obtain and , and according to and realize the division of the content of the i-th volatile active ingredient in the control gas, is the dependent variable for the equation to hold, and the source data includes , , and .

[0011] Furthermore, the cross-phase component tracing method includes: Obtain the third liquid and the mass M1 of the third liquid from the separated liquid, pour a quantitative control gas into the third liquid, and liquefy the control gas in the third liquid to obtain a supplementary liquid and the mass M2 of the supplementary liquid. Detect the supplementary liquid by HPLC fingerprint technology to obtain the effective ingredient data of the supplementary liquid; Calculate the matching degree D between the effective ingredient data of the supplementary liquid and the effective ingredient data of the initial liquid phase by the matching calculation method, and calculate and obtain the source data of the control gas by combining the matching degrees A, B, and D through the source calculation method.

[0012] Furthermore, the matching calculation method includes: The matching degree D includes the matching degree DX between the volatile active ingredients X and the matching degree DY between the non-volatile active ingredients Y; Obtain and , and are the contents of the i-th volatile active ingredient in the supplementary liquid and in the initial liquid phase respectively, and are the contents of the j-th non-volatile active ingredient in the supplementary liquid and in the initial liquid phase respectively, represents the matching degree of the i-th volatile active ingredient between the supplementary liquid and the initial liquid phase, Indicates the matching degree of the j-th non-volatile active ingredient between the replenishing liquid and the initial liquid phase; The source calculation method includes: Based on and correct the partitioning ratio to obtain and , and according to and realize the partitioning of the content of the i-th volatile active ingredient in the control gas, and according to and realize the partitioning of the content of the j-th non-volatile active ingredient in the control gas, and are the dependent variables for the equation to hold, and the source data includes , , and .

[0013] Furthermore, the gas detection method includes obtaining a quantitative gas sample of the control gas, cooling the quantitative gas sample, liquefying the water vapor in the quantitative gas sample to obtain a reference gas and a reference liquid, detecting and obtaining the mass of the reference gas and the mass of the reference liquid, and then respectively detecting the active ingredients in the reference liquid and the reference gas to obtain reference liquid active ingredient data and reference gas active ingredient data, and obtaining control gas active ingredient data based on the reference liquid active ingredient data, the reference gas mass, the reference gas active ingredient data, and the reference liquid mass.

[0014] An active ingredient detection device for a liquid beverage that dredges meridians and improves microcirculation adopts the above-mentioned active ingredient detection method for a liquid beverage that dredges meridians and improves microcirculation. The detection device includes: A microchannel member, at the bottom end of the microchannel member is fixedly connected with an ultrasonic device for emitting orthogonal ultrasonic waves. At the feeding end and the discharging end of the microchannel member are respectively fixedly connected with one-way restricting members. Inside the microchannel member is provided with a microchannel, and at the bottom end of the microchannel member is fixedly connected with a particle collection chamber; An electric field device for applying a gradient electric field to the liquid inside the microchannel member is installed inside the microchannel member; An alternating tank body is fixedly installed at both ends of the microchannel member. The inside of the alternating tank body communicates with the microchannel inside the microchannel member, and a piston is connected inside the alternating tank body; A filter membrane member for intercepting solids is fixedly installed inside the microchannel; An optical detector for detecting the particle content in the liquid passing through the inside of the microchannel member is fixed inside the microchannel member.

[0015] Compared with the prior art, the beneficial effects of the present invention are as follows: The method and device for detecting the active ingredients of the liquid beverage for dredging meridians and improving microcirculation analyze the matching degrees A between the reference gas and the initial liquid-phase active ingredient data and B between the reference gas and the initial solid-phase active ingredient data through the cross-phase-state ingredient tracing method. Then, the matching degrees A and B are corrected through the matching calculation method and the source calculation method, thereby realizing the tracing and classification of the active ingredients between different phases, and realizing the classification of the active ingredient content of the reference gas through the cross-phase-state ingredient tracing method, so as to further realize the acquisition of the distribution of the active ingredient content on the basis of accurately obtaining the active ingredient content in the liquid beverage.

[0016] Meanwhile, through the organic combination of the matching calculation method and the source calculation method, the distribution and content of the active ingredients in the liquid beverage are determined. This method effectively reduces the problem of incorrect detection results of ingredient data caused by phase interference, making the detection results more reliable. Through the cross-phase-state ingredient tracing method, the present detection method realizes the detection of the active ingredients in the solid phase and the gas phase, which helps to comprehensively understand the distribution and content of the active ingredients in the liquid beverage and provides a comprehensive basis for quality control.

[0017] Through the source calculation method, the ingredient data in different phases are obtained, and traced through the matching calculation method. The second gas is obtained through partial vaporization operation and compared with the reference gas, thereby realizing the correction of the volatile active ingredients. The reference gas is introduced into the third liquid and liquefied to obtain the supplementary liquid, which is compared with the initial liquid phase, thereby realizing the correction of the volatile and non-volatile active ingredients. The matching degree C further corrects the source data by comparing the ingredient differences between the second gas and the reference gas, and the matching degree D realizes the correction of the non-volatile active ingredients by comparing the ingredient differences between the supplementary liquid and the initial liquid phase, thus ensuring the reliability of the source data. BRIEF DESCRIPTION OF THE DRAWINGS

[0018] Figure 1 It is a schematic diagram of the cross-phase-state ingredient tracing method of the present invention; Figure 2 It is a schematic diagram of Embodiment 1 of the present invention; Figure 3 It is a schematic diagram of Embodiment 2 of the present invention; Figure 4 It is an isometric view of the detection device of the present invention; Figure 5 It is a cross-sectional view of the microchannel component of the present invention.

[0019] In the figure: 1. Microchannel component; 2. Alternating tank body; 3. Filter membrane component; 4. Optical detector; 5. Unidirectional limiting component; 6. Ultrasonic device; 7. Electric field device; 8. Particle collection chamber. Detailed implementation manners

[0020] Next, the technical solutions in the embodiments of the present invention will be clearly and completely described in conjunction with the accompanying drawings in the embodiments of the present invention. Obviously, the described embodiments are only a part of the embodiments of the present invention, rather than all the embodiments. All other embodiments obtained by those of ordinary skill in the art based on the embodiments of the present invention without creative efforts shall fall within the protection scope of the present invention.

[0021] As Figure 1 - Figure 5 shown, the present invention provides a technical solution: a method for detecting the active ingredients of a liquid beverage for dredging meridians and improving microcirculation. The detection method includes: S01. Obtain a liquid beverage sample, separate the solid phase and the liquid phase in the liquid beverage sample by means of a circulation filtration method, apply orthogonal ultrasonic waves to the liquid beverage during the separation process, collect the separated liquid phase and solid phase respectively, and detect the separated liquid phase to obtain initial liquid phase active ingredient data; S02. Perform a drying operation on the separated solid phase, obtain the control gas discharged during the drying operation, homogenize the dried solid phase, and detect and obtain initial solid phase active ingredient data; S03. Process the control gas by means of a cross-phase state ingredient tracing method to obtain the source data of the control gas, correct the initial solid phase active ingredient data and the initial liquid phase active ingredient data according to the source data, divide the control gas active ingredient data through the source data to obtain the corresponding solid phase content data and the corresponding gas phase content data, and combine the corresponding gas phase content data with the initial liquid phase active ingredient data and the initial solid phase active ingredient data respectively according to the source data to obtain the solid phase active ingredient data and the liquid phase active ingredient data, and obtain the active ingredient distribution data based on the solid phase active ingredient data and the liquid phase active ingredient data; S04. Jointly map the solid phase active ingredient data and the liquid phase active ingredient data on the liquid beverage to generate a distribution map and data of the active ingredients in the liquid beverage; The cross-phase state ingredient tracing method includes; Detect the active ingredients of the control gas by means of a gas detection method to obtain the control gas active ingredient data; Calculate the matching degree A between the control gas active ingredient data and the initial liquid phase active ingredient data and the matching degree B between the control gas active ingredient data and the initial solid phase active ingredient data by means of a matching calculation method, and calculate and obtain the source data of the control gas by combining the matching degree A and the matching degree B through a source calculation method.

[0022] The matching calculation method includes: The active ingredients are classified into volatile active ingredient X and non-volatile active ingredient Y according to the volatility attributes of the active ingredients, and Xi and Yj are obtained by classification according to the quantity and volatility attributes of the active ingredients. i represents the i-th volatile active ingredient, and j represents the j-th non-volatile active ingredient. i (1, 2, 3... m), where m represents the quantity of volatile active ingredients, and j (1, 2, 3... n), where n represents the quantity of non-volatile active ingredients; The matching degree A includes the matching degree AX between volatile active ingredients X and the matching degree AY between non-volatile active ingredients Y; The matching degree BA includes the matching degree BX between volatile active ingredients X and the matching degree BY between non-volatile active ingredients Y; Obtain 、 、 And , And respectively represent the content of the i-th volatile component and the content of the j-th non-volatile component in the initial liquid-phase active ingredient data, And respectively represent the content of the i-th volatile component and the content of the j-th non-volatile component in the control gas active ingredient data, And respectively represent the content of the i-th volatile component and the content of the j-th non-volatile component in the initial solid-phase active ingredient data, And respectively match the matching degree of the i-th volatile component and the matching degree of the j-th non-volatile component in the matching degree A; The source calculation method includes: Obtain source data based on the matching degree A And , And respectively match the matching degree of the i-th volatile component and the matching degree of the j-th non-volatile component in the matching degree B. The source data includes 、 、 And , And represent the partitioning ratio between the liquid phase and the solid phase in the content of the i-th volatile component in the control gas, And represent the partitioning ratio between the liquid phase and the solid phase in the content of the j-th volatile component in the control gas.

[0023] The cross-phase-state component traceability method includes: ​​Obtain the second liquid from the separated liquid phase by using a filtration membrane extraction method, perform partial vaporization on the second liquid to obtain the second gas, and detect the second gas by using a gas detection method to obtain the data of the effective components of the second gas; Calculate the matching degree C between the data of the effective components of the second gas and the data of the effective components of the control gas by using a matching calculation method, and calculate and obtain the source data of the control gas by using a source calculation method in combination with the matching degrees A, B, and C.

[0024] 4. According to the method for detecting the effective components of a liquid beverage for dredging meridians and improving microcirculation as claimed in claim 3, the matching calculation method includes: The matching degree C includes the matching degree CX between the volatile effective components X and the matching degree CY between the non-volatile effective components Y; , and are respectively the content of the i-th volatile effective component in the control gas and the content in the second gas, represents the matching degree of the i-th volatile effective component between the second gas and the control gas; The source calculation method includes: Based on correct the partitioning ratio to obtain and , and according to and realize the partitioning of the content of the i-th volatile effective component in the control gas, is the dependent variable for realizing the equality to hold, and the source data includes , , and .

[0025] The cross-phase component tracing method includes: Obtain the third liquid and the mass M1 of the third liquid from the separated liquid, pour a quantitative control gas into the third liquid, and liquefy the control gas in the third liquid to obtain the supplementary liquid and the mass M2 of the supplementary liquid, and detect the supplementary liquid by using the HPLC fingerprint technology to obtain the data of the effective components of the supplementary liquid; Calculate the matching degree D between the data of the effective components of the supplementary liquid and the data of the effective components of the initial liquid phase by using a matching calculation method, and calculate and obtain the source data of the control gas by using a source calculation method in combination with the matching degrees A, B, and D.

[0026] The matching calculation method includes: The matching degree D includes the matching degree DX between the volatile effective components X and the matching degree DY between the non-volatile effective components Y; Obtain and , and are respectively the content of the i-th volatile active ingredient in the supplementary liquid and the content in the initial liquid phase, and are respectively the content of the j-th non-volatile active ingredient in the supplementary liquid and the content in the initial liquid phase, represents the matching degree of the i-th volatile active ingredient between the supplementary liquid and the initial liquid phase, represents the matching degree of the j-th non-volatile active ingredient between the supplementary liquid and the initial liquid phase; The source calculation method includes: Based on and correct the division ratio to obtain and , and according to and realize the division of the content of the i-th volatile active ingredient in the control gas, and according to and realize the division of the content of the j-th non-volatile active ingredient in the control gas, and are the dependent variables for the equation to hold, and the source data includes , , and .

[0027] The gas detection method includes obtaining a quantitative gas sample of the control gas, cooling the quantitative gas sample, liquefying the water vapor in the quantitative gas sample to obtain a reference gas and a reference liquid, detecting and obtaining the mass of the reference gas and the mass of the reference liquid, then respectively detecting the active ingredients in the reference liquid and the reference gas to obtain reference liquid active ingredient data and reference gas active ingredient data, and obtaining control gas active ingredient data based on the reference liquid active ingredient data, the reference gas mass, the reference gas active ingredient data and the reference liquid mass.

[0028] An effective ingredient detection device for a liquid beverage for dredging meridians and improving microcirculation adopts the above-mentioned effective ingredient detection method for a liquid beverage for dredging meridians and improving microcirculation. The detection device includes: Channel member 1, the bottom end of the micro-channel member 1 is fixedly connected with an ultrasonic device 6 for emitting orthogonal ultrasonic waves, the feeding end and the discharging end of the micro-channel member 1 are respectively fixedly connected with one-way limiting members 5, the inside of the micro-channel member 1 is provided with a micro-channel, and the bottom end of the micro-channel member 1 is fixedly connected with a particle collection chamber 8; The electric field device 7 for applying a gradient electric field to the liquid inside the microchannel component 1 is installed inside the microchannel component 1; The alternating tank body 2 is fixedly installed at both ends of the microchannel component 1. The inside of the alternating tank body 2 communicates with the microchannels inside the microchannel component 1, and a piston is connected inside the alternating tank body 2; The filter membrane component 3 for intercepting solid phases is fixedly installed inside the microchannel; The optical detector 4 for detecting the particle content in the liquid passing through the inside of the microchannel component 1 is fixed inside the microchannel component 1.

[0029] When detecting the active ingredients of the liquid beverage for dredging meridians and improving microcirculation, a drying operation is performed on the solid phase to obtain gas active ingredient data. A cross-phase state ingredient tracing method is used for the gas active ingredient data to obtain the source of the gas active ingredient data, and the gas active ingredient data is divided according to the source, so as to correct the obtained initial solid-phase active ingredient data and initial liquid-phase active ingredient data to obtain solid-phase active ingredient data and liquid-phase active ingredient data. This method can not only detect the content of the active ingredients of the microcirculation liquid beverage, but also obtain the content distribution of the active ingredients. For the content of a single active ingredient, it comes from the solid phase and the liquid phase. Therefore, the content of the active ingredient needs to be divided, so as to understand the distribution of the effective content in the liquid beverage on the basis of obtaining the effective content of the liquid beverage, clarify the distribution of the effective content, ensure the reasonable ratio and stability of these active ingredients in the beverage, and thus ensure the effect of improving microcirculation. The active ingredients in the liquid can be absorbed, and the active ingredients absorbed and adsorbed in the particles can supplement the active ingredients in the liquid. A liquid beverage refers to a beverage that has no definite shape, has a solid content of less than or equal to 5%, and is easy to flow.

[0030] In the device, the liquid beverage for opening meridians and improving microcirculation is moved into the alternating tank body 2, and the liquid beverage is pushed through the microchannel in the microchannel component 1 by the piston inside the alternating tank body 2. The electric field device 7 applies a gradient electric field, and the ultrasonic device 6 emits an orthogonal ultrasonic wave. Due to the pressure, the liquid beverage passes through the microchannel to the inside of another alternating tank body 2, and the piston in the other alternating tank body 2 can suck the liquid beverage by applying negative pressure by moving the piston. Under the action of the gradient electric field, the particles will move to the retention area in the microchannel, that is, the particle collection chamber 8, and be intercepted by the filter membrane component 3. Then, the piston in the other alternating tank body 2 changes the piston movement direction, so that the liquid beverage is filtered out from the other alternating tank body 2 on the microchannel component 1. A microchannel flows through, and the one-way flow of the microchannel is achieved through the one-way restriction piece 5, and the original alternating tank body 2 absorbs the liquid beverage to achieve circulation filtration until the optical detector detects zero particles 4, and then collects the particles, and removes the residual liquid on the particles through a drying operation to obtain the control gas and the effective component data of the control gas, and then the effective component data of the control gas is divided through the detection method to obtain the effective component content and distribution of the liquid beverage for opening the meridians and improving microcirculation. The microchannel limits the internal height, but does not limit the length and width. Therefore, the present application uses a microchannel piece 1 with a longer length to achieve gradient electric field separation.

[0031] The liquid beverage for dredging meridians and improving microcirculation may contain tanshinone, eucalyptus oil, safflower yellow, cinnamaldehyde, ferulic acid, vitamin E, sea buckthorn and ligustilide, among which volatile components include ligustilide and eucalyptus oil. Since the formulas of specific products may be different, the specific volatile components may also be different. When the specific ingredients in the liquid beverage for dredging meridians and improving microcirculation are clarified, the HPLC fingerprint technology corresponding to the effective ingredients can be used to detect the effective ingredients in the liquid. For the solid phase in the liquid beverage for dredging meridians and improving microcirculation, for example, sea buckthorn can be crushed by a wall breaking machine and put into the liquid beverage, or traditional Chinese medicine such as salvia miltiorrhiza can be crushed and put into the liquid beverage, retaining the powder (granular) form to exert intestinal effects. The invention relates to a liquid beverage which has no definite shape, has a solid content of less than or equal to 5%, and is easy to flow. Therefore, it is necessary not only to detect the components in the liquid, but also to detect the solids. In order to achieve the effect of improving microcirculation by dredging meridians and improving microcirculation without any harm, it is reasonable for the liquid beverage to contain solid powder (particles). For example, there are pulp particles in the existing orange juice liquid beverage, but it will not affect its drinking. At the same time, the present application mainly realizes the detection of effective ingredients, and regards the remaining non-effective ingredients as the same substance, so as to calculate the effective ingredients and compare the effective ingredient data.

[0032] In this application, data on the active ingredients in the solid phase are obtained through spectral analysis, the active ingredients in the liquid phase are detected by HPLC fingerprint technology, and the active ingredients in the gas are detected by gas chromatography-mass spectrometry. HPLC is high-performance liquid chromatography, which is a highly efficient separation and analysis method based on liquid chromatography technology and is widely used in fields such as chemistry, pharmacy, food, and the environment for separating, identifying, and quantifying chemical components in complex mixtures. HPLC fingerprint technology is a quality control method that combines high-performance liquid chromatography (HPLC) with chemical pattern recognition analysis. Its core principle is to separate the chemical components in a complex liquid sample by HPLC and generate a chromatogram with characteristic peak information, and then systematically evaluate the quality consistency of the sample by calibrating the common peaks, calculating the similarity, etc. The active ingredients (such as flavonoids, saponins, phenolic acids, etc.) and potential functional factors of the liquid beverage for dredging meridians and improving microcirculation are detected for multiple components simultaneously and quality-controlled by using HPLC fingerprint technology. Through characteristic peak calibration and chemical pattern recognition, a consistency evaluation system between batches is established.

[0033] The gradient electric field separation technology realizes the solid-liquid separation by applying a non-uniform electric field and using the differences in dielectric constant, conductivity, or charge between the particles and the liquid phase to make the solid-phase particles migrate in the electric field gradient. Neutral particles move due to the polarization effect in the non-uniform electric field, and the direction depends on the difference in dielectric properties between the particles and the medium. Coatings such as polyethylene glycol set on the inner wall of the microchannel can further reduce the adsorption of particles inside the channel. At the same time, on the basis of the gradient electric field separation technology, the separation efficiency is further improved by setting a filter membrane to intercept the solid phase, and the filter membrane allows the liquid phase to pass through.

[0034] The optical detector 4 captures the particle migration trajectory and particle size change in real time through the high-speed microscopic camera embedded in the optical detection layer on the microchannel, and dynamically adjusts the electric field and ultrasonic parameters in combination with the image analysis algorithm until the solid-liquid two-phase is completely separated. The high-speed microscopic camera is installed on the microchannel and can distinguish particles and liquid based on the different absorption degrees of particles and liquid to light, so as to realize the detection of the number of particles in the liquid. Based on the repeated cycle process, when no particles are detected in the microchannel multiple times, the filtration can be stopped.

[0035] During the liquid sampling process, a sampler equipped with a 0.22μm filter membrane is used to block the solid-phase particles from entering the sampler to ensure the purity of the sampled liquid. Orthogonal ultrasound, as a kind of ultrasound with a special vibration mode, has its vibration direction perpendicular to the propagation direction, forming a transverse wave, which can reduce the boundary layer thickness and increase the mass transfer rate, and helps the adsorbed components diffuse from the particle surface to the liquid. Orthogonal ultrasound treatment can be carried out simultaneously during the separation process to improve the separation effect.

[0036] Since there will be a large amount of water vapor in the reference gas and the second gas, and the water vapor will affect the detection of the effective components in the gas by the mass spectrometer. Therefore, when detecting the effective components, it is necessary to reduce the water vapor in the reference gas and the second gas. The reference gas can be cooled to obtain the reference liquid and the reference gas, and then the effective components in the reference liquid and the reference gas are detected respectively. The detection of the second gas is the same. The gas detection method includes processing the reference gas by a cooling method to obtain the reference liquid and the reference gas. While obtaining the reference liquid and the reference gas, the masses of the reference liquid and the reference gas are obtained synchronously. The reference liquid and the reference gas are detected respectively by the effective component detection method to obtain the reference liquid effective component data and the reference gas effective component data. Based on the reference liquid effective component data and the reference gas effective component data, the reference gas effective component data is obtained. When performing the above operations, the samples are operated in batches. The detection of the effective components requires sampling, and all detections in this article are sampling detections to avoid the excessive time consumption caused by overall detection and the existence of repeated detections, so as to reduce the error caused by single detection.

[0037] The source data of the content of the effective components in the reference gas can be initially obtained by matching degree A and matching degree B. Then, the source data obtained by matching degree A and matching degree B is corrected through Example 1 and Example 2, so as to ensure the accuracy of the division of the content of the effective components in the reference gas.

[0038] The initial solid-phase effective component data is obtained by detecting the solid-phase particles after drying operation, while the initial liquid-phase effective component data is obtained by detecting the separated liquid phase.

[0039] Example 1, as Figure 2 shown, perform a partial vaporization operation on the second liquid. The partial vaporization operation will not vaporize all the liquids in the sample, but only perform a partial vaporization operation. The liquid phases before and after the vaporization operation need to be weighed respectively to obtain the amount of liquid lost during the vaporization operation. Based on the detection of the gas after the vaporization operation, the effective component data of the gas and the amount of liquid lost are obtained. Since the partial vaporization operation will cause the volatile effective components to form gas, the second gas is thus obtained, so as to realize the comparison between the second gas and the reference gas. Since it is a comparison between gases, Example 1 can only realize the correction of the source of volatile effective components. And considering that when drying the separated solid phase, the non-volatile components in the residual liquid will form a solid phase, so there may be no non-volatile components in the gas. Therefore, the source data of the volatile effective components is corrected through Example 1.

[0040] The matching degree C is obtained by comparing the second gas and the reference gas. Since it is a comparison of gases, only the and Correction to obtain and , and then according to and to achieve the division of the content of the i-th volatile active ingredient in the reference gas, according to and to achieve the division of the content of the j-th volatile active ingredient in the reference gas, so as to obtain the content and distribution of a single active ingredient.

[0041] For example, using Salvia miltiorrhiza, Salvia miltiorrhiza is a medicinal material for activating blood circulation and regulating menstruation, which can improve blood circulation. It contains tanshinone inside, and tanshinone has the effect of activating blood circulation and regulating menstruation. During detection, the content of tanshinone in the initial solid-phase active ingredient data is 0.50 mg / g, the content of tanshinone in the initial liquid-phase active ingredient data is 1.20 mg / L, and the content of tanshinone in the reference gas active ingredient data is 0.10 mg / L. Among them, tanshinone can be regarded as a volatile active ingredient. In order to calculate the values in the initial solid-phase active ingredient data and the reference gas active ingredient data, it is necessary to perform unit conversion on the initial liquid-phase active ingredient data and the reference gas active ingredient data. The density of the liquid phase detected is 1.00 g / L, so as to obtain 1.20 mg / g and 0.10 mg / g.

[0042] , , = , = , through and to achieve the division of the content of tanshinone in the reference gas active ingredient, where is divided into the initial liquid-phase active ingredient data and combined, is divided into the initial solid-phase active ingredient data and combined, so as to obtain the solid-phase active ingredient data and the liquid-phase active ingredient data. At this time, i = 1, indicating that tanshinone is the content of the first volatile component.

[0043] Perform the calculation of Example 1. The detection of the active ingredients of the second gas and the detection of the active ingredients of the first gas are both under the same air pressure. The content of tanshinone in the active ingredient data of the second gas is 0.08 mg / L. Since the content of tanshinone in the second gas is less than the content of tanshinone in the reference gas, there is tanshinone in the solid phase. Therefore, correct the original source ratio, = , substitute into the formula, where Since it is to make the equation hold all the time, when makes change, by adjusting to make the equation hold all the time. Similarly and will also ensure that the equation always holds, obtaining = , = , through and realize the division of tanshinone content. The division of tanshinone content means the presence of tanshinone in the control gas is from the liquid phase, from the solid phase. Combine the tanshinone from the solid phase with the tanshinone content obtained by detecting the solid phase, so as to obtain the tanshinone content in the solid phase, reducing the situation of missing the detected fixed content caused by the formation of gas by tanshinone after the drying operation process.

[0044] Perform the calculation of Example 2, and obtain that the tanshinone content in the data of the effective components of the supplementary liquid is 1.25 mg / L, where the mass ratio before and after the mixing of the gas and the liquid phase, detected to obtain = 1.02, then = , and then obtain = 0.31, = 0.69. Through the division of tanshinone content by 0.31 and 0.69, the source division of the non-volatile content is the same. Example 1 and Example 2 are mainly used for the comparison between the liquid phase and the gas phase. Therefore and are preferably applied to the source division related to the liquid phase.

[0045] Since Example 1 is the comparison between the control gas and the No. 2 gas, and since the volatile effective components are easily volatilized into gas, Example 1 can only realize the correction of the matching parameters of the volatile effective components. Relative to Example 1 which can only realize the correction of the matching parameters related to the volatile effective components, Example 2 can also correct the non-volatile effective components.

[0046] Example 2, as Figure 3 shown, introduce the control gas into the No. 3 liquid, so that the control gas can start to liquefy in the No. 3 liquid. Therefore, the temperature of the No. 3 liquid will be reduced, so that the control gas can be quickly cooled and liquefied, and at the same time, avoid the generation of particulate matter during the liquefaction process. If particulate matter is generated, the No. 3 liquid can be quantitatively supplemented. The control gas is absorbed by the No. 3 liquid to obtain the supplementary liquid, and the effective components of the supplementary liquid are detected to obtain the data of the effective components of the supplementary liquid. The generation of particulate matter indicates the saturation of a certain component inside the supplementary liquid, which will affect the subsequent detection of the supplementary liquid, resulting in the detection result of this component being less than the actual value. Therefore, it is necessary to avoid the generation of particulate matter.

[0047] The change of the volatile active ingredient is achieved by the absorption of the reference gas by the third liquid. At the same time, due to the change of the mass composition of the third liquid, such as the conversion of water vapor into water, the content of the non-volatile active ingredient in the third liquid will also change. Based on this change, the ideal content of the non-volatile active ingredient in the residual gas is judged, so as to correct the source data of the content of the non-volatile active ingredient.

[0048] Compare the active ingredient data of the supplementary liquid with the initial liquid phase active ingredient data. Calculate the matching degree D between the active ingredient data of the supplementary liquid and the initial liquid phase active ingredient data through the matching calculation method, analyze the differences between the two, and then the influence of the reference gas on the initial liquid phase can be clarified. According to and realize the division of the content of the i-th volatile active ingredient in the reference gas. Through and realize the division of the content of the j-th non-volatile active ingredient in the reference gas, so as to obtain the content and distribution of a single active ingredient.

[0049] Through the organic combination of the matching calculation method and the source calculation method, the distribution and content of the active ingredients in the liquid beverage are determined. This method effectively reduces the problem of incorrect detection results of the component data caused by phase interference, making the detection results more reliable. This detection method realizes the detection of the active ingredients in the solid phase and the gas phase through the cross-phase component tracing method, which helps to comprehensively understand the distribution and content of the active ingredients in the liquid beverage and provides a comprehensive basis for quality control. Through the source calculation method, the component data in different phases are obtained, and through the matching calculation method for tracing, the second gas is obtained through partial vaporization operation and compared with the reference gas, so as to realize the correction of the volatile active ingredient. By introducing the reference gas into the third liquid and liquefying it, the supplementary liquid is obtained and compared with the initial liquid phase, so as to realize the correction of the volatile active ingredient and the non-volatile active ingredient. The matching degree C further corrects the source data by comparing the component differences between the second gas and the reference gas, and the matching degree D realizes the correction of the non-volatile active ingredient by comparing the component differences between the supplementary liquid and the initial liquid phase, thus ensuring the reliability of the source data.

[0050] The matching calculation method lies in calculating the matching degree of active ingredients between different phases (such as gas phase, liquid phase, solid phase). This method first divides the active ingredients into volatile active ingredients (such as certain substances in mushrooms) and non-volatile active ingredients according to their volatility attributes, and then calculates the matching degrees of these ingredients between different phases respectively. The matching degree A represents the matching degree between the active ingredient data of the control gas and the initial liquid phase active ingredient data, and the matching degree B represents the matching degree between the active ingredient data of the control gas and the initial solid phase active ingredient data. For volatile and non-volatile ingredients, the matching degrees can be further subdivided and calculated (such as AX, AY, BX, BY, etc.). These matching degrees are obtained by precisely comparing the contents of the same ingredients in different phases. Through the cross-phase ingredient traceability method, the matching degree A between the control gas and the initial liquid phase active ingredient data and the matching degree B between the control gas and the initial solid phase active ingredient data are analyzed. Then, through the matching calculation method and the source calculation method, the matching degrees A and B are corrected, thereby realizing the traceability and division of active ingredients between different phases, and through the cross-phase ingredient traceability method, the division of the active ingredient content of the control gas is realized, achieving the distribution of the active ingredient content on the basis of obtaining the active ingredient content in the liquid beverage.

[0051] The source calculation method is based on the results of the matching calculation method to further determine the source of the active ingredients in the control gas. This method calculates the source data of the control gas by comprehensively considering the matching degree A, the matching degree B, and based on the matching degree C between the second gas and the control gas mentioned in Example 1, and the matching degree D between the supplementary liquid and the initial liquid phase mentioned in Example 2. According to the values of these matching degrees, the content of the active ingredients in the control gas can be scientifically and reasonably divided, so as to determine the source of these active ingredients. This division is realized based on the matching degree of active ingredients between different phases, so it can more accurately reflect the real situation. Through the matching calculation method and the source calculation method, on the basis of obtaining the active ingredient content in the liquid beverage, the distribution of the active ingredient content in the beverage can be obtained.

[0052] In this application, the volatilization property of the active ingredient is defined as follows: during the vaporization operation or the drying operation, the unvaporized active ingredient is regarded as the non-volatile active ingredient, and the vaporized active ingredient is regarded as the volatile active ingredient. Both the vaporization operation and the drying operation are to remove moisture. However, compared with the direct operation method in the vaporization operation, since the drying operation will operate on the particles, it is necessary to avoid the influence of rapid temperature rise on the particles. The vaporization operation is to directly raise the temperature to vaporize the liquid, and the drying operation is to provide a temperature and ensure that the temperature remains unchanged to remove the residual liquid existing on the solid phase. Since the data of the component content between the solid phase and the gas phase is not convenient to directly compare, while the gas phase and the liquid phase can be converted for comparison, and the comparison between the two can be realized by converting the gas phase into the liquid phase and the liquid phase into the gas phase. Therefore, in Example 1 and Example 2, attention is paid to comparing and calculating the liquid and the gas, respectively using the conversion from the gas phase to the liquid phase and the conversion from the liquid phase to the gas phase, and then performing the comparison calculation to achieve the correction of the source data. The source data can be initially obtained through the matching degree A and the matching degree B, but there will be a situation where the active ingredient is adsorbed on the particles. Therefore, the methods in Example 1 and Example 2 are needed to achieve the correction of the source data.

[0053] Although the embodiments of the present invention have been shown and described, for those of ordinary skill in the art, it can be understood that various changes, modifications, substitutions, and variations can be made to these embodiments without departing from the principles and spirit of the present invention. The scope of the present invention is defined by the appended claims and their equivalents.

Claims

1. A method for detecting the effective ingredients of a liquid beverage for dredging meridians and improving microcirculation, the method comprising: S01, obtaining a liquid beverage sample, using a circulating filtration method to separate a solid phase and a liquid phase in the liquid beverage sample, applying orthogonal ultrasonic waves to the liquid beverage during the separation process, collecting the separated liquid phase and solid phase respectively, detecting the separated liquid phase, and obtaining initial liquid phase effective component data; S02, performing a drying operation on the separated solid phase, obtaining a reference gas discharged during the drying operation, homogenizing the dried solid phase, and detecting and obtaining data on the effective components of the initial solid phase; It is characterized in that, S03, the reference gas is processed by a cross-phase component tracing method to obtain the source data of the reference gas, the initial solid phase effective component data and the initial liquid phase effective component data are corrected according to the source data, the corresponding gas phase content data is combined with the initial liquid phase effective component data and the initial solid phase effective component data according to the source data, solid phase effective component data and liquid phase effective component data are obtained, and effective component distribution data are obtained based on the solid phase effective component data and the liquid phase effective component data; S04, jointly mapping the solid phase effective ingredient data and the liquid phase effective ingredient data onto the liquid beverage to generate a distribution map and data of the effective ingredients in the liquid beverage; The cross-phase component tracing method comprises: Detecting the effective components of the reference gas by a gas detection method to obtain the effective component data of the reference gas; The matching degree A between the reference gas effective component data and the initial liquid phase effective component data and the matching degree B between the reference gas effective component data and the initial solid phase effective component data are calculated by a matching calculation method, and the source data of the reference gas is obtained by combining the matching degree A and the matching degree B through a source calculation method.

2. The method for detecting the effective ingredients of a liquid beverage for dredging meridians and improving microcirculation according to claim 1, characterized in that: The matching calculation method comprises: According to the volatility of the active ingredients, the active ingredients are divided into volatile active ingredients X and non-volatile active ingredients Y, and Xi and Yj are obtained according to the number and volatility of the active ingredients. i represents the volatile i-th volatile active ingredient, j represents the volatile j-th non-volatile active ingredient, i (1, 2, 3...m), m represents the number of volatile active ingredients, j (1, 2, 3...n), n represents the number of non-volatile active ingredients; The matching degree A includes the matching degree AX between the volatile active ingredients X and the matching degree AY between the non-volatile active ingredients Y; The matching degree BA includes the matching degree BX between the volatile active ingredients X and the matching degree BY between the non-volatile active ingredients Y; get , , and , and It represents the content of the i-th volatile component and the j-th non-volatile component in the initial liquid phase effective component data. and They represent the content of the i-th volatile component and the j-th non-volatile component in the effective component data of the reference gas, respectively. and They represent the i-th volatile component content and the j-th non-volatile component content in the initial solid phase effective component data, and The matching degree of the i-th volatile component and the matching degree of the j-th non-volatile component in the matching degree A are matched respectively; The source calculation method includes: Get source data based on matching degree A and , and The matching degree of the i-th volatile component and the j-th non-volatile component in the matching degree B are respectively matched. The source data include , , and , and represents the partition ratio between the liquid phase and the solid phase in the content of the i-th volatile component in the reference gas, and It represents the partition ratio between the liquid phase and the solid phase in terms of the content of the jth volatile component in the reference gas.

3. The method for detecting the effective ingredients of a liquid beverage for dredging meridians and improving microcirculation according to claim 2, characterized in that: The cross-phase component tracing method comprises: A filter membrane extraction method is used to obtain liquid No. 2 from the separated liquid phase, and a partial vaporization operation is performed on the liquid No. 2 to obtain gas No.

2. The gas No. 2 is detected by a gas detection method to obtain data on the effective components of the gas No. 2; The matching degree C between the effective component data of gas No. 2 and the effective component data of the reference gas is calculated by a matching calculation method, and the source data of the reference gas is obtained by combining the matching degrees A, B and C by a source calculation method.

4. The method for detecting the effective ingredients of a liquid beverage for dredging meridians and improving microcirculation according to claim 3, characterized in that: The matching calculation method comprises: The matching degree C includes the matching degree CX between the volatile active ingredients X and the matching degree CY between the non-volatile active ingredients Y; , and are the contents of the ith volatile active ingredient in the control gas and in the second gas, respectively. It represents the matching degree of the i-th volatile active ingredient between gas No. 2 and the reference gas; The source calculation method includes: based on Correct the division ratio and obtain and ,in accordance with and The value of realizes the division of the content of the i-th volatile effective component in the reference gas, is the dependent variable for the equation to be established, and the source data include , , and .

5. The method for detecting the effective ingredients of a liquid beverage for dredging meridians and improving microcirculation according to claim 2, characterized in that: The cross-phase component tracing method comprises: Obtaining liquid No. 3 and the mass M1 of liquid No. 3 from the separated liquid, pouring a certain amount of reference gas into liquid No. 3, and liquefying the reference gas in liquid No. 3, obtaining supplementary liquid and the mass M2 of supplementary liquid, and detecting the supplementary liquid by HPLC fingerprint technology to obtain data of effective components of the supplementary liquid; The matching degree D between the supplementary liquid effective component data and the initial liquid phase effective component data is calculated by a matching calculation method, and the source data of the control gas is obtained by combining the matching degrees A, B and D by a source calculation method.

6. The method for detecting the effective ingredients of a liquid beverage for dredging meridians and improving microcirculation according to claim 5, characterized in that: The matching calculation method comprises: The matching degree D includes the matching degree DX between the volatile active ingredients X and the matching degree DY between the non-volatile active ingredients Y; get and , and are the contents of the i-th volatile active ingredient in the supplementary liquid and in the initial liquid phase, respectively, and are the contents of the j-th non-volatile active ingredient in the supplementary liquid and in the initial liquid phase, respectively, represents the matching degree of the i-th volatile active ingredient between the supplementary liquid and the initial liquid phase, represents the matching degree of the jth non-volatile active ingredient between the supplementary liquid and the initial liquid phase; The source calculation method includes: based on and Correct the division ratio and obtain and ,in accordance with and The value of realizes the division of the content of the i-th volatile active ingredient in the reference gas, according to and The value of realizes the division of the content of the jth non-volatile effective component in the reference gas, and is the dependent variable for the equation to be established, and the source data include , , and .

7. The method for detecting the effective ingredients of a liquid beverage for dredging meridians and improving microcirculation according to claim 1, characterized in that: The gas detection method includes obtaining a quantitative gas sample of a control gas, cooling the quantitative gas sample, liquefying water vapor in the quantitative gas sample, obtaining a reference gas and a reference liquid, and detecting and obtaining the mass of the reference gas and the mass of the reference liquid, and then respectively detecting the effective components in the reference liquid and the reference gas to obtain reference liquid effective component data and reference gas effective component data, and obtaining the control gas effective component data based on the reference liquid effective component data, the reference gas mass, the reference gas effective component data and the reference liquid mass.

8. A device for detecting effective components of a liquid beverage for dredging meridians and improving microcirculation, which adopts a method for detecting effective components of a liquid beverage for dredging meridians and improving microcirculation according to any one of claims 1 to 7, characterized in that: The detection device comprises: A microchannel member (1), wherein the bottom end of the microchannel member (1) is fixedly connected to an ultrasonic device (6) for emitting orthogonal ultrasonic waves, the feed end and the discharge end of the microchannel member (1) are respectively fixedly connected to a one-way limiting member (5), a microchannel is arranged inside the microchannel member (1), and the bottom end of the microchannel member (1) is fixedly connected to a particle collection chamber (8); An electric field device (7) for applying a gradient electric field to the liquid inside the microchannel member (1), the electric field device (7) being installed inside the microchannel member (1); An alternating tank body (2), the alternating tank body (2) being fixedly mounted at both ends of the microchannel member (1), the interior of the alternating tank body (2) being in communication with the microchannel inside the microchannel member (1), and the interior of the alternating tank body (2) being connected to a piston; A filter membrane (3) for retaining a solid phase is fixedly installed inside the microchannel; An optical detector (4) for detecting the particle content in the liquid passing through the microchannel member (1) is fixed inside the microchannel member (1).

Images