Method for identifying and evaluating high-temperature-resistant and drought-resistant grape variety

Abstract

The invention relates to the field of fruit tree drought tolerance identification, in particular to an identification and evaluation method of high-temperature and drought resistant grape varieties, which comprises the following steps: preparing a greenhouse, and constructing a planting and high-temperature and drought environment in the greenhouse; a pollination insect box is arranged in the greenhouse before the initial flowering stage of the grapes; a plurality of image collectors are prepared, the image collectors are bonded to the sides, close to branches, of grape inflorescences, and the image collectors are used for collecting the interaction situation of pollination insects and the grape inflorescences and the growth situation of grape leaves; preparing a controller which is used for judging the total duration of the grape inflorescence collected by the image collector and the pollination insects based on image recognition and counting the total duration of interaction of the pollination insects on each inflorescence of each variety of grapes; and evaluating the high-temperature drought resistance of the grapes according to the growth conditions of the grapes, including nutrition and health of tree bodies, photosynthetic efficiency of leaves, fruit development, survival rate, morbidity, fruit quality and total interaction duration of pollination insects.

Description

Technical Field

[0001] The present invention relates to the field of drought tolerance identification of fruit trees, and specifically to an identification and evaluation method for grape varieties with high temperature and drought tolerance. Background Art

[0002] High temperature and drought are the most important stress factors restricting grape yield and quality. With the continuous intensification of drought caused by global warming, it will seriously affect the healthy and sustainable development of the grape industry in China. Therefore, exploring drought-resistant and heat-tolerant grape germplasm resources is of great significance for improving the drought and heat tolerance of grapes. High temperature and drought stress will not only lead to water deficit in grape plants, affecting photosynthesis, but also cause difficulties in fruit setting, resulting in reduced yields, insufficient fruit fragrance, uneven coloring, burning, water loss and other problems. In severe cases, leaf scorching, rachis drying, serious yield reduction, and even plant death will occur. Therefore, by evaluating and screening excellent drought-resistant and heat-tolerant grape germplasm materials, special excellent gene resources can be provided for grape breeding, which can be used for grape germplasm innovation and genetic improvement. For example, the 'Boleite' grape can still grow normally under high temperature and drought conditions, and its yield is stable, showing strong heat resistance and drought tolerance. The evaluation methods for grape varieties with high temperature and drought tolerance mainly include direct identification methods and indirect identification methods, and comprehensive evaluation is carried out by combining multi-dimensional indicators such as morphology, physiology, biochemistry and molecular biology.

[0003] Field identification method: Under natural conditions, drought stress is formed by controlling irrigation to evaluate the drought resistance of grape varieties. This method is simple but greatly affected by environmental factors.

[0004] Pot drought method: By controlling the soil water content to simulate drought conditions, the growth and physiological responses of plants are measured. It is the most widely used drought resistance identification method at present.

[0005] Hypertonic solution method: Using hypertonic solutions such as PEG6000 or mannitol to simulate drought stress, which is suitable for drought resistance identification at the seedling stage.

[0006] Morphological structure identification method: Including the degree of root development, leaf cell structure, photosynthetic efficiency, etc., which reflects the morphological adaptation ability of plants to drought.

[0007] Physiological and biochemical identification method: Such as leaf water potential, osmotic adjustment substances (proline, malondialdehyde, soluble sugar), antioxidant enzyme activities (SOD, POD, CAT), etc., which are used to evaluate the physiological drought resistance mechanism of plants.

[0008] Molecular biology method: By using molecular marker techniques (such as RFLP, SNP, SSR) to locate drought-related genes (QTLs), providing a theoretical basis for molecular breeding.

[0009] However, the main economic value of grapes comes from the fruits. In addition to evaluating the fruit quality, the flowering season of grapes coincides with the stage of rapid temperature rise. High temperature not only affects pollen viability, but also affects the evaporation of water on the stigma surface, reduces stigma secretions, affects pollen attachment, and decreases the pollination efficiency of pollinating insects. At the same time, high temperature also reduces the activity of pollinating insects and the number of pollinations. If the grapes are not pollinated, the fruits will not grow, and other means need to be used for assisted pollination, increasing the planting cost. Therefore, when evaluating the high-temperature and drought tolerance of grapes, it is also necessary to judge the interaction effect between grape plants and pollinating insects under high-temperature and drought conditions to obtain the adaptability of grapes in high-temperature and drought environments. Summary of the Invention

[0010] To solve the above problems, the present invention provides a method for identifying and evaluating high-temperature and drought-tolerant grape varieties, which is used to evaluate the adaptability of grapes under high-temperature and drought conditions by detecting the interaction effect between pollinating insects and grape inflorescences.

[0011] To achieve the above object, the technical solution of the present invention is as follows: A method for identifying and evaluating high-temperature and drought-tolerant grape varieties includes:

[0012] Step 1: Prepare a greenhouse. There is a temperature control component in the greenhouse, which is used to change the temperature in the greenhouse. Prepare several grape planting pots in the greenhouse, and the grape planting pots are used to provide the soil environment for grape planting.

[0013] Step 2: Plant the grapes of the variety to be identified and evaluated in the grape planting pots respectively. Control the temperature in the greenhouse by the temperature control component to simulate high-temperature conditions, and control the irrigation amount into the grape planting pots to simulate drought conditions.

[0014] Step 3: Set up a pollinating insect box in the greenhouse before the initial flowering stage of the grapes. The pollinating insect box is used to provide the source of pollinating insects in the greenhouse. Prepare several image collectors, and bond the image collectors to one side of the grape inflorescence close to the branch. The image collectors are used to collect the interaction situation between pollinating insects and grape inflorescences.

[0015] Step 4: Prepare a controller, and the controller is connected to the image collectors by signals. The controller is used to judge the total duration of the grape inflorescences and pollinating insects collected by the image collectors based on image recognition, and count the total interaction duration of pollinating insects on each inflorescence of each variety of grapes.

[0016] Step 5: Comprehensively evaluate the high-temperature and drought tolerance of the grapes by comprehensively analyzing the grape growth conditions, including tree body nutrition and health, leaf photosynthetic efficiency, fruit development, survival rate, disease occurrence, fruit quality, and the total interaction duration of pollinating insects.

[0017] The above scheme has the following beneficial effects:

[0018] 1. In this solution, a greenhouse is used to simulate a high-temperature and drought environment, which is convenient for users to control variables, can construct high-temperature and drought performances with different gradients year by year, and is convenient for evaluating grape varieties under different degrees of high-temperature and drought.

[0019] 2. In this solution, an image collector is adhered to one side of the grape inflorescence close to the branch to collect the interaction duration between pollinating insects and the grape inflorescence. This interaction duration can reflect the attractiveness of the current variety of inflorescence to pollinating insects, thereby evaluating the probability of successful pollination and fruit setting of grapes under high-temperature and drought conditions, and can reflect the environmental adaptability after comprehensively considering the behavior of pollinating insects under high-temperature and drought conditions, rather than being limited to the growth and development performance of the grapes themselves.

[0020] 3. In this solution, due to the differences in flower organ types and pollen properties among different varieties, there will be cross-pollination phenomena. Therefore, only evaluating the fruit set rate cannot fully reflect the pollination ability of grape varieties themselves. By statistically analyzing the interaction duration between pollinating insects and grape inflorescences, the attracting effect of grape inflorescences on pollinating insects can be measured more accurately, thereby evaluating the pollination performance under high-temperature and drought conditions.

[0021] Furthermore, in step one, the distance between grape planting pots is 3 - 5 m, and humidity sensors and temperature sensors are installed in each grape planting pot.

[0022] Beneficial effects: Inflorescences attract pollinating insects through fragrance. Therefore, if grapes are planted too close to each other, they will interfere with each other. It is necessary to keep an appropriate distance between grape planting pots to reduce interference. Humidity sensors and temperature sensors can detect the humidity and temperature of the planting soil, which is convenient for recording data and analysis. Since there is a difference between ground temperature and air temperature, temperature sensors also need to be installed in the grape planting pots.

[0023] Furthermore, a solar simulation light source is installed in the greenhouse, and the solar simulation light source compensates for the light intensity in the high-temperature environment.

[0024] Beneficial effects: The greenhouse can directly utilize external sunlight, but the greenhouse itself has heat preservation ability, which will cause the light intensity and natural temperature to not match. In simulating high-temperature and drought, the temperature needs to be increased most of the time. Therefore, a solar simulation light source is set up for compensation to make the temperature and light intensity match.

[0025] Furthermore, the temperature regulation of the temperature regulation component follows the seasonal law and the temperature change law generated by the sun rising and setting within a single day.

[0026] Beneficial effects: High temperatures do not exist in every growth stage of grapes. Therefore, the simulated high-temperature environment also needs to change following natural laws, enabling grape varieties that enter the full-bloom stage earlier or later to avoid the influence of the high-temperature season. At the same time, there are also temperature changes caused by the rising and setting of the sun within a single day, allowing some grape varieties with special biological clocks to avoid the influence of daytime high temperatures, thereby improving pollination performance.

[0027] Furthermore, in step three, the pollination insect boxes are evenly arranged around each grape planting pot. Different pollination insects with different active time periods are provided inside the pollination insect boxes, and the proportion of each pollination insect is the same as that in the natural environment.

[0028] Beneficial effects: Different insects have different active time periods. Setting a variety of pollination insects that meet the proportion in the natural environment can conform to natural laws and simulate a more restored pollination scenario in the greenhouse.

[0029] Furthermore, in step three, after setting the pollination insect boxes before the initial flowering stage, the pollination insects are fed manually. After the initial flowering stage begins, the number of pollination insect corpses in the greenhouse is checked and collected every day, and the number of pollination insect corpses is supplemented according to the number of pollination insect corpses, so that the number of pollination insects remains at a preset value.

[0030] Beneficial effects: The flower quantity is relatively small at the initial flowering stage. Therefore, manual feeding is used to ensure the activity of the pollination insects. After the initial flowering stage begins, by checking and collecting the pollination insect corpses, the change in the number of pollination insects is judged and supplemented to meet the pollination requirements.

[0031] Furthermore, in step three, before bonding the image collector, a flower thinning operation is carried out to remove the weak flowers and retain the strong ones.

[0032] Beneficial effects: The development effect of weak branches is not only poor, but they also consume nutrients. Therefore, they are not used as a reference standard. Therefore, a flower thinning operation is carried out before bonding the image collector to ensure that the collected inflorescences can meet the normal development requirements.

[0033] Furthermore, pollen samples of each grape variety are collected during the full-bloom stage of grapes. The viscosity of each pollen sample is detected in the laboratory, and the adhesion test of the pollen to pollination insects is carried out to estimate the adhesion performance of the pollen. In step five, the total interaction time of the pollination insects is combined with the adhesion performance of the pollen to evaluate the high-temperature and drought tolerance.

[0034] Beneficial effects: The pollination rate is also affected by the viscosity of the pollen. That is, the pollination performance is actually jointly determined by the pollen viscosity and the total interaction time of the pollination insects. By sampling to estimate the adhesion performance of the pollen, the high-temperature and drought tolerance is evaluated in combination with the total interaction time of the pollination insects.

[0035] Furthermore, the image collector is a day-night dual-use camera, and power supply wires are provided for each image collector, and the power supply wires are bonded along the grape branches.

[0036] Beneficial effects: The day-and-night dual-purpose camera ensures the ability to record the activities of nocturnal pollinating insects. The power supply wire is adhered along the grape branches to reduce the impact of the power supply wire on pollinating insects.

[0037] Furthermore, artificial pollination is carried out on the grape inflorescences before the end of the full bloom period.

[0038] Beneficial effects: Since the fruit set rate cannot reflect the self-pollination ability of grape varieties, artificial pollination is carried out on the grape inflorescences before the end of the full bloom period, so that there are more fruit samples when evaluating the quality of grape fruits subsequently.

[0039] Additional aspects and advantages of the present invention will be given in part in the following description, become apparent in part from the following description, or be learned through the practice of the present invention. Description of the Drawings

[0040] Figure 1 It is a schematic diagram of the steps of an embodiment of the method for identifying and evaluating heat-resistant and drought-resistant grape varieties of the present invention;

[0041] Figure 2 It is an axonometric schematic diagram of an image collector of an embodiment of the method for identifying and evaluating heat-resistant and drought-resistant grape varieties of the present invention;

[0042] Figure 3 It is a side top view schematic diagram of an image collector of an embodiment of the method for identifying and evaluating heat-resistant and drought-resistant grape varieties of the present invention.

[0043] Reference numerals in the drawings of the specification include: 1, camera; 2, power supply wire; 3, triangular support frame; 4, first adhesive tape; 5, second adhesive tape. Detailed Embodiments

[0044] The technical solutions of the present invention will be clearly and completely described below in conjunction with the drawings. Obviously, the described embodiments are part of the embodiments of the present invention, rather than all of them. All other embodiments obtained by those of ordinary skill in the art based on the embodiments of the present invention without creative efforts shall fall within the protection scope of the present invention.

[0045] In the description of the present invention, it should be noted that the orientation or positional relationship indicated by the terms "center", "upper", "lower", "left", "right", "vertical", "horizontal", "inner", "outer", etc. is based on the orientation or positional relationship shown in the drawings. It is only for the convenience of describing the present invention and simplifying the description, rather than indicating or implying that the device or element referred to must have a specific orientation, be constructed and operated in a specific orientation, and therefore should not be construed as a limitation of the present invention. In addition, the terms "first", "second", "third" are only used for descriptive purposes and cannot be understood as indicating or implying relative importance.

[0046] In the description of the present invention, it should be noted that unless otherwise clearly specified and limited, the terms "installed", "connected", "connected" should be understood in a broad sense. For example, it can be a fixed connection, a detachable connection, or an integral connection; it can be a mechanical connection or an electrical connection; it can be directly connected or indirectly connected through an intermediate medium, and it can be the communication inside two elements. For those of ordinary skill in the art, the specific meanings of the above terms in the present invention can be understood according to specific circumstances.

[0047] The following is a further detailed description through specific embodiments:

[0048] As shown in the attached Figures 1 - 3 figures: The method for identifying and evaluating heat-resistant and drought-tolerant grape varieties includes:

[0049] Step 1: Prepare a greenhouse. There is a temperature control component in the greenhouse. The temperature control component is an electric heater, which is used to change the temperature in the greenhouse. There is a sun simulation light source in the greenhouse, and the sun simulation light source compensates for the light intensity in the high-temperature environment additionally. The temperature control of the temperature control component follows the seasonal law and the temperature change law generated by the sun's rise and fall within a single day. Prepare several grape planting pots in the greenhouse. The grape planting pots are used to provide a soil environment for grape planting. The distance between the grape planting pots is 3-5m. Humidity sensors and temperature sensors are installed in each grape planting pot.

[0050] Step 2: Plant the grapes of the variety to be identified and evaluated in the grape planting pots respectively. Control the temperature in the greenhouse by the temperature control component to simulate high-temperature conditions, and control the irrigation amount into the grape planting pots to simulate drought conditions;

[0051] Step 3: Before the initial flowering stage of the grapes, set up pollination insect boxes in the greenhouse. The pollination insect boxes are used to provide the source of pollination insects in the greenhouse. The pollination insect boxes are evenly set around each grape planting pot. Different pollination insects with different active time periods are placed in the pollination insect boxes, and the proportion of each pollination insect is the same as that in the natural environment. Prepare several image collectors and bond the image collectors to the side of the grape inflorescence close to the branch. The image collectors are used to collect the interaction between the pollination insects and the grape inflorescence.

[0052] Step 4: Prepare a controller. The controller is connected to the image collectors by signals. The controller is used to judge the total duration of the grape inflorescence and the pollination insects collected by the image collectors based on image recognition, and to count the total interaction duration of the pollination insects on each inflorescence of each grape variety. Collect pollen samples of each grape variety during the full-bloom stage of the grapes, detect the viscosity of each pollen sample in the laboratory, and conduct a test on the attachment of pollination insects to the pollen to estimate the attachment performance of the pollen.

[0053] Step 5: Comprehensively evaluate the high-temperature and drought tolerance of the grapes by comprehensively analyzing the grape growth conditions, including the tree body nutrition and health, leaf photosynthetic efficiency, fruit development, survival rate, disease incidence, fruit quality, and the total interaction duration of the pollination insects.

[0054] The greenhouse can build a stable environment. Compared with using the wild environment, it can reduce uncertain factors, so as to stably evaluate the grape performance. The greenhouse can isolate natural rainfall, which is convenient for users to control the irrigation amount of the grapes and build drought conditions. At the same time, the temperature control component can regulate the temperature, thus stably building high-temperature conditions.

[0055] The greenhouse can directly utilize the external sunlight, but the greenhouse itself has heat preservation ability, which will cause the sunlight intensity and the natural temperature to not match. In the simulation of high temperature and drought, the temperature needs to be increased for most of the time. Therefore, a solar simulation light source is set up for compensation to make the temperature and the light intensity match. Moreover, the high-temperature intensity and occurrence time every day in summer also fluctuate. The decrease in the night temperature will also provide an opportunity for the grapes to resume normal growth. Therefore, it is difficult to fully and truly reflect the heat tolerance of the grapes in the natural environment only by simulating high-temperature climate indoors.

[0056] Because grapes often face more variable and complex environments in the fields, and high temperature does not exist in every growth stage of the grapes, the simulated high-temperature environment also needs to change according to the natural laws, so as to avoid the influence of the high-temperature season during the full-bloom stage of each grape variety. At the same time, the daily temperature change and the high-temperature occurrence time also fluctuate with the movement track of the sun, enabling some grape varieties with special biological clocks to avoid the influence of daytime high temperature, thus improving the pollination performance.

[0057] The active periods of different insects vary. Setting multiple types of pollinating insects that meet the proportions in the natural environment can conform to the natural laws and simulate a more realistic pollination scenario in greenhouse. The flower quantity is less at the initial flowering stage, so artificial feeding of sugar is used to ensure the activity of pollinating insects. After the initial flowering stage starts, the number of pollinating insects is judged by checking and collecting their corpses, and supplements are given to meet the pollination requirements.

[0058] The image collector is bonded to one side of the grape inflorescence close to the branch to collect the interaction duration between pollinating insects and the grape inflorescence. In theory, pollen fertilization occurs through the action of wind and pollinating insects. Pollen will attach to pollinating insects and thus be transferred between different flowers. Due to the significant reduction of pollen viscosity and pollinating insect activity affected by high temperature, the stronger the pollination performance of grapes, the higher the interaction frequency between pollinating insects and the inflorescence. Better results may only be achieved in high-temperature and arid environments. Flower shape, fragrance, sweetness, etc. can all change the attracting ability of the inflorescence to pollinating insects. At the same time, the time of the full-bloom stage, the biological clock of flower opening and closing, plant morphology, etc. can all affect the interaction times and duration between pollinating insects and the inflorescence. This interaction duration can reflect the attracting ability of the inflorescence of the current variety to pollinating insects, thereby evaluating the probability of successful pollination of grapes to obtain fruits under high-temperature and arid conditions, and can reflect the environmental adaptability after comprehensively considering the behavior of pollinating insects under high-temperature and arid conditions, rather than being limited to the growth and development performance of the grapes themselves.

[0059] Due to the different pollen properties among different varieties, cross-pollination may occur. Therefore, only evaluating the fruit set rate cannot reflect the pollination ability of grape varieties themselves. By counting the interaction duration between pollinating insects and grape inflorescences, the attracting effect of grape inflorescences on pollinating insects can be measured more accurately, thereby evaluating the pollination performance under high-temperature and arid conditions.

[0060] In step three, before bonding the image collector, thinning of flowers is carried out to remove weak ones and keep strong ones.

[0061] The development effect of inferior branches is not good and they will also consume nutrients, so they are not used as reference standards. Therefore, thinning of flowers is carried out before bonding the image collector to ensure that the collected inflorescences meet the correct development requirements.

[0062] The image collector is a day-night dual-use camera 1. The bottom of the camera 1 is detachably connected with a first adhesive patch 4 conforming to the shape of the grape vine. A triangular support frame 3 is bonded and fixed to the back of the camera 1. The image collectors are all provided with a power supply wire 2, and second adhesive patches 5 are evenly arranged on the power supply wire 2. The power supply wire 2 is bonded along the grape branch through the second adhesive patch 5.

[0063] The day-night dual-use camera 1 ensures that the activities of pollinating insects at night can be recorded. The power supply wire 2 is bonded along the grape branch to reduce the influence of the power supply wire 2 on pollinating insects.

[0064] Artificially pollinate the grape inflorescences before the end of the full bloom period.

[0065] Since the fruit set rate cannot fully reflect the self-pollination ability of grape varieties, artificially pollinate the grape inflorescences before the end of the full bloom period to have more fruit samples for subsequent evaluation of grape fruit quality. In addition, since the fruit is the main source of economic production, if the economic effect brought by the quantity and quality of the fruit is greater than the cost of artificial pollination, it can also be evaluated as an excellent grape variety with high temperature and drought tolerance.

[0066] The inflorescences attract pollinating insects through fragrance, so if grape plants are planted too close to each other, they will affect each other. It is necessary to keep an appropriate distance between grape planting pots to reduce interference. Humidity sensors and temperature sensors can detect the humidity and temperature of the planting soil, which is convenient for recording data and analysis. Since there is a difference between the ground temperature and the air temperature, temperature sensors also need to be set inside the grape planting pots.

[0067] The pollination rate is also affected by pollen viscosity, that is, the pollination performance is actually determined by the combination of pollen viscosity and the total interaction time of pollinating insects. Estimate the attachment performance of pollen by sampling, and then evaluate the high temperature and drought tolerance in combination with the total interaction time of pollinating insects.

[0068] Obviously, the above-mentioned implementation cases are only examples clearly described and not limitations on the implementation methods. For those of ordinary skill in the art, other different forms of changes or variations can be made based on the above description. It is not necessary and impossible to enumerate all the implementation methods here. And the obvious changes or variations derived therefrom are still within the protection scope of the present invention.

Claims

1. A method for identifying and evaluating high temperature and drought resistant grape varieties, characterized in that: include: Step 1: prepare a greenhouse, wherein a temperature control component is provided in the greenhouse, and the temperature control component is used to change the temperature in the greenhouse. A number of grape planting pots are prepared in the greenhouse, and the grape planting pots are used to provide a soil environment for grape planting; Step 2: Plant the grape varieties to be identified and evaluated in the grape planting pots respectively, control the temperature of the greenhouse by the temperature control component to simulate high temperature conditions, and control the irrigation amount into the grape planting pots to simulate drought conditions; Step 3: before the grapes begin to bloom, a pollinating insect box is set up in the greenhouse, and the pollinating insect box is used to provide a source of pollinating insects in the greenhouse; a plurality of image collectors are prepared, and the image collectors are bonded to the side of the grape inflorescence close to the branches, and the image collectors are used to collect the interaction between the pollinating insects and the grape inflorescence; Step 4, prepare a controller, which is connected to the image collector signal. The controller is used to determine the total time of the grape inflorescence collected by the image collector and the pollinating insects based on image recognition, and count the total interaction time of the pollinating insects on each inflorescence of each grape variety; Step five: Comprehensively evaluate the grape's resistance to high temperature and drought by comprehensively analyzing the grape's growth conditions, including tree nutritional health, leaf photosynthetic efficiency, fruit development, survival rate, disease incidence, fruit quality, and the total duration of interaction with pollinating insects.

2. The method for identifying and evaluating high temperature and drought resistant grape varieties according to claim 1, characterized in that: In step 1, the distance between the grape planting pots is 3-5m, and humidity sensors and temperature sensors are provided in the grape planting pots.

3. The method for identifying and evaluating high temperature and drought resistant grape varieties according to claim 2, characterized in that: The greenhouse is equipped with a solar simulation light source, which additionally compensates for the light intensity in a high temperature environment.

4. The method for identifying and evaluating high temperature and drought resistance grape varieties according to claim 3, characterized in that: The temperature control of the temperature control component follows the seasonal laws and the temperature changes caused by the rising and falling of the sun within a single day.

5. The method for identifying and evaluating high temperature and drought resistant grape varieties according to claim 4, characterized in that: In step 3, pollinating insect boxes are evenly arranged around each grape planting pot, and pollinating insects with different active time periods are arranged in the pollinating insect boxes, and the proportion of each pollinating insect is the same as that in the natural environment.

6. The method for identifying and evaluating high temperature and drought resistance grape varieties according to claim 5, characterized in that: In step three, a pollination insect box is set up before the beginning of flowering and pollination insects are raised by artificial feeding. After the beginning of flowering, the number of pollination insect corpses in the greenhouse is checked and collected daily, and the number of pollination insect corpses is supplemented according to the number of pollination insect corpses to keep the number of pollination insects at a preset value.

7. The method for identifying and evaluating high temperature and drought resistance grape varieties according to claim 6, characterized in that: In step three, before bonding the image collector, flower thinning is performed to remove weak ones and retain strong ones.

8. The method for identifying and evaluating high temperature and drought resistance grape varieties according to claim 7, characterized in that: Pollen samples of various grape varieties were collected during the peak flowering period of grapes, the viscosity of each pollen sample was tested in the laboratory, and the pollen adhesion test of pollinating insects was carried out to estimate the adhesion performance of pollen. In step five, the total interaction time of pollinating insects was combined with the adhesion performance of pollen to evaluate the high temperature and drought resistance.

9. The method for identifying and evaluating high temperature and drought resistance grape varieties according to claim 8, characterized in that: The image collector is a day and night dual-purpose camera (1), and each image collector is provided with a power supply line (2), and the power supply line (2) is bonded along the grape branches.

10. The method for identifying and evaluating high temperature and drought resistant grape varieties according to claim 9, characterized in that: The grape inflorescences are artificially pollinated before the end of the flowering period.

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