Preparation method of bionic scaffold with micro-nano structure regulation and control

Abstract

The invention relates to the technical field of curved surface bionic structure manufacturing, in particular to a preparation method of a bionic stent with a micro-nano structure regulation and control function. Comprising the following steps: S1, preparing a core body outer layer corresponding to the bionic stent; the method specifically comprises the following steps: S101, acquiring image file data of a to-be-replaced curved surface structure; s102, performing model reconstruction on the image file obtained in the step S101, and obtaining three-dimensional model information of the real curved surface curvature of the curved surface structure to be replaced; s103, performing noise removal, normal phase repair and packaging operation on the three-dimensional model information to obtain a structure model; s104, importing the structural model into reverse reconstruction software, and performing structural design of the outer layer of the core body; s105, manufacturing an outer layer of the designed core body; s2, placing the outer layer of the core body prepared in the step S1 on a rotating shaft platform to complete the preparation of the bionic stent; the mechanical property of the prepared bionic stent can be regulated and controlled, and the construction of a complex bionic stent is ensured.

Description

Technical Field

[0001] The present invention relates to the technical field of manufacturing curved bionic structures, and in particular to a method for preparing a bionic scaffold with micro-nano structure regulation. Background Art

[0002] The human body contains many curved tissues, including the trachea, blood vessels, urethra, heart valves, etc. When there are conditions such as stenosis or malignant tumors in the human curved tissues, tissue replacement of the diseased area is an effective solution. Currently, the sources of optional curved tissue substitutes mainly include autologous, allogeneic, tissue engineering scaffolds, etc. Autologous curved tissue replacement will cause damage to the donor area, and some tissues do not have autologous replaceability. Allogeneic curved tissue replacement may have the risks of immune rejection and disease transmission. And tissue engineering scaffolds, also known as bionic scaffolds, can construct tissue engineering curved structures with specific morphologies, which is a new idea for tissue replacement.

[0003] Traditional construction methods of bionic scaffolds include phase separation method, freezing method, porogen leaching method, lamination method, etc. However, these methods cannot achieve the shape bionics of bionic scaffolds. More importantly, they cannot guarantee the mechanical properties of the constructed bionic scaffolds, nor can they guarantee the construction of complex bionic scaffold structures.

[0004] Therefore, there is an urgent need to provide a method for preparing a bionic scaffold with micro-nano structure regulation, which can guarantee the mechanical properties of the constructed bionic scaffold and the construction of complex bionic scaffold structures compared with the prior art. Summary of the Invention

[0005] The present invention solves the technical problems existing in the prior art, and provides a method for preparing a bionic scaffold with micro-nano structure regulation.

[0006] To achieve the above object, the technical solution adopted by the present invention is as follows:

[0007] A method for preparing a bionic scaffold with micro-nano structure regulation includes the following steps:

[0008] S1. Prepare the outer layer of the core corresponding to the bionic scaffold; specifically, it includes the following steps:

[0009] S101. Obtain the image file data of the curved surface structure to be replaced;

[0010] S102. Use medical reconstruction software to reconstruct the model of the image file obtained in step S101, and obtain the three-dimensional model information of the true surface curvature of the curved surface structure to be replaced;

[0011] S103. Use triangular patch optimization software to perform noise removal, normal repair, and encapsulation operations on the three-dimensional model information obtained in step S102, and obtain the structural model;

[0012] S104. Import the structural model obtained in S103 into reverse reconstruction software to perform the structural design of the outer layer of the core body;

[0013] S105. Manufacture the designed outer layer of the core body;

[0014] S2. Place the outer layer of the core body prepared in S1 on the rotating shaft platform, and use a 3D printing device and the rotating shaft platform to complete the preparation of the bionic scaffold corresponding to the surface structure to be replaced.

[0015] Furthermore, the structural model described in step S103 includes the surface structure to be replaced and its extended part structure;

[0016] S104 specifically includes the following steps:

[0017] S1041. In the reverse reconstruction software, perform fitting and design according to the surface curvature of the surface structure adjacent to the surface structure to be replaced to obtain a digital model that matches the surface structure adjacent to the surface structure to be replaced. This digital model is the macroscopic size of the outer layer of the core body;

[0018] S1042. Design the texture structure of the outer layer of the core body by setting lattice units on the outer surface of the digital model.

[0019] Even further, S1042 is specifically represented as follows:

[0020] (1) Set the type of lattice unit, including at least triangle, square, honeycomb, and rhombus;

[0021] (2) Set the size of the lattice unit. It is set that all lattice units have an inscribed circle feature, and the size of the lattice unit is set by the diameter of the inscribed circle, including the following situations:

[0022] 1) The diameter of the inscribed circle is greater than or equal to 0 μm and less than 100 μm;

[0023] 2) The diameter of the inscribed circle is greater than or equal to 100 μm and less than 500 μm;

[0024] 3) The diameter of the inscribed circle is greater than or equal to 500 μm and less than 1000 μm;

[0025] 4) The diameter of the inscribed circle is greater than or equal to 1000 μm and less than 1500 μm;

[0026] 5) The diameter of the inscribed circle is greater than or equal to 1500 μm;

[0027] (3) Set the spacing of the lattice units, specifically including the following situations:

[0028] 1) The spacing is greater than 0 mm and less than or equal to 0.1 mm;

[0029] 2) The spacing is greater than 0.1 mm and less than or equal to 2 mm;

[0030] 3) The spacing is greater than 2 mm and less than or equal to 5 mm;

[0031] 4) The spacing is greater than 5 mm;

[0032] (4) Set the three-dimensional shape and surface shape of the lattice unit, including the following situations:

[0033] 1) All lattice units are in a convex shape, and the surface shape is set to a rounded corner or an acute angle;

[0034] 2) All lattice units are in a concave shape, and the surface shape is set to a rounded corner or an acute angle;

[0035] 3) The lattice units are alternately set in a convex shape and a concave shape, and the surface shape is set to a rounded corner or an acute angle;

[0036] (5) Set the height or depth of the lattice unit. When the lattice unit is in a convex shape, set its height. When the lattice unit is in a concave shape, set its depth.

[0037] Further, the rotary shaft platform includes a motor assembly and a rotary shaft mandrel. The motor assembly includes a first motor, a second housing, and a second motor; the output end of the first motor is connected to the second housing, the second motor is installed inside the second housing, the output end of the second motor is connected to the rotary shaft mandrel, and the outer wall of the rotary shaft mandrel is sleeved with an outer layer of the core body.

[0038] Further, a thermostat is installed inside the rotary shaft mandrel. The thermostat includes a first thermostat and a second thermostat. The first thermostat is installed inside the rotary shaft mandrel in a high-temperature temperature control mode, and the second thermostat is installed inside the rotary shaft mandrel in a low-temperature temperature control mode.

[0039] Further, the first thermostat uses a single-head heating tube.

[0040] Further, the second thermostat includes a fastener, a refrigerant pipe, a heat sink, and a superconducting column. The end of the refrigerant pipe is installed on the fastener. A refrigerant channel is provided inside the refrigerant pipe. The outer wall of the refrigerant pipe is sleeved with the heat sink, and the superconducting column is sleeved outside the heat sink.

[0041] Further, the temperature control range of the first thermostat is 25 - 500 °C; the temperature control range of the second thermostat is -40 - 25 °C; the temperature control accuracy of both the first thermostat and the second thermostat is ±0.1 °C.

[0042] Further, S2 specifically includes the following steps:

[0043] S201. Place the outer layer of the core body outside the rotating shaft mandrel, and align the nozzle of the printing device with the outer layer of the core body.

[0044] S202. Start the first motor. According to the specific external shape of the prepared bionic scaffold, change the angle between the outer layer of the core body and the ground, or start the second motor alone to make the outer layer of the core body rotate around its own axis, or start the second motor simultaneously to make the outer layer of the core body rotate around its own axis.

[0045] Furthermore, the manufacturing and forming process of the outer layer of the core body includes subtractive processes, cutting processes, additive manufacturing processes, DLP stereolithography technology, LCD structured light forming technology, SLA, and SLM.

[0046] Compared with the prior art, the beneficial effects of the present invention are as follows:

[0047] (1) According to the curved surface structure to be replaced, in the process of preparing the corresponding outer layer of the core body, through reverse modeling software, a macroscopic shape identical to the outer contour of the curved surface structure to be replaced is obtained, which is the outer layer of the core body. Then, by setting the lattice units on the outer surface of the outer layer of the core body, the roughness and porosity of the prepared bionic scaffold can be controlled, which helps to regulate the mechanical properties of the bionic scaffold and can also ensure the construction of a complex bionic scaffold structure.

[0048] (2) According to the shape of the bionic scaffold to be printed, start the first motor to change the angle between the outer layer of the core body and the ground, or start the second motor to make the outer layer of the core body rotate around its own axis, or start the first motor and the second motor simultaneously. Macroscopically, the printing trajectory is increased, and the outer layer of the core body has multiple degrees of freedom when printing the bionic scaffold. Microscopically, it also has the effect of directionally regulating the mechanical strength of the bionic scaffold, and thus diverse curved surface morphologies can be formed. Description of the Drawings

[0049] Figure 1 is a flowchart of the method of the present invention.

[0050] Figure 2 is an exploded view of the overall structure of the rotating shaft platform of the present invention.

[0051] Figure 3 is a schematic diagram showing the connection relationship between the rotating shaft mandrel and the temperature controller in the high-temperature temperature control mode of the present invention.

[0052] Figure 4 is a sectional view showing the connection relationship between the rotating shaft mandrel and the temperature controller in the low-temperature temperature control mode of the present invention.

[0053] Figure 5 is the present invention and Figure 1A sectional view showing the internal connection relationship of different motor components.

[0054] Figure 6 It is an overall schematic diagram of the outer layer of the core body and the bionic stent finally formed by the artificial blood vessel of the present invention.

[0055] Figure 7 It is a schematic diagram of the bionic stent finally formed by the artificial blood vessel of the present invention.

[0056] Figure 8 It is a three-dimensional view of the bionic stent finally formed by the artificial heart valve of the present invention.

[0057] Explanation of reference numerals:

[0058] 1. Shock-absorbing bottom plate; 2. First outer shell; 3. Second outer shell; 4. First motor; 5. Connecting sleeve; 6. Second motor; 7. Rotating shaft mandrel; 8. Thermostat; 81. Fastener; 82. Refrigerant pipe; 83. Refrigerant channel; 84. Heat sink; 85. Superconducting column; 9. Outer layer of the core body; 91. Groove; 92. Protrusion; 10. Worm; 11. Worm gear; 12. Motor base. Detailed implementation manners

[0059] The technical solutions of the present invention will be clearly described below with reference to the accompanying drawings. Obviously, the described embodiments are not all embodiments of the present invention. All other embodiments obtained by those of ordinary skill in the art without creative efforts shall fall within the protection scope of the present invention. It should be noted that the orientation or positional relationship indicated by the terms "center", "upper", "lower", "left", "right", "vertical", "horizontal", etc. is based on the orientation or positional relationship shown in the accompanying drawings, and is only for the convenience of describing the present invention and simplifying the description, rather than indicating or implying that the device or element referred to must have a specific orientation, be constructed and operated in a specific orientation, and therefore should not be construed as a limitation of the present invention.

[0060] As Figure 1 shown, the present invention provides a method for preparing a bionic stent with micro-nano structure regulation, including the following steps:

[0061] S1. Prepare the outer layer of the core body corresponding to the bionic stent; specifically including the following steps:

[0062] S101. Obtain data of the surface structure to be replaced through CT, MRI, Micro-CT, and the data format is not limited to image files in Dicom format;

[0063] S102. Use medical reconstruction software to reconstruct the model of the image file obtained in S101, obtain the three-dimensional model information of the true surface curvature of the surface structure to be replaced, and output the data in the form of triangular patches in STL format;

[0064] S103. Use the triangular facet optimization software to perform operations such as noise removal, normal repair, and encapsulation on the 3D model information obtained in step S102 to obtain a structural model. The structural model includes the surface structure to be replaced and the surface structure of the adjacent part of the surface structure to be replaced, and the structural model is in STL format;

[0065] S104. Import the structural model obtained in S103 into the reverse reconstruction software and perform the design of the outer layer of the core body, specifically including the following steps:

[0066] S1041. In the reverse reconstruction software, perform fitting and design according to the surface curvature of the surface structure of the adjacent part of the surface structure to be replaced to obtain a digital model that matches the surface structure of the adjacent part of the surface structure to be replaced. The digital model is the macroscopic size of the outer layer of the core body;

[0067] S1042. Design the texture structure of the outer layer of the core body by setting lattice units on the outer surface of the digital model, specifically including the following steps:

[0068] (1) Set the types of lattice units, including at least triangles, squares, honeycombs, and rhombuses;

[0069] (2) Set the size of the lattice units. It is set that all lattice units have an inscribed circle feature, and the size of the lattice units is set by the diameter of the inscribed circle, including the following situations:

[0070] 1) The diameter of the inscribed circle is greater than or equal to 0 μm and less than 100 μm;

[0071] 2) The diameter of the inscribed circle is greater than or equal to 100 μm and less than 500 μm;

[0072] 3) The diameter of the inscribed circle is greater than or equal to 500 μm and less than 1000 μm;

[0073] 4) The diameter of the inscribed circle is greater than or equal to 1000 μm and less than 1500 μm;

[0074] 5) The diameter of the inscribed circle is greater than or equal to 1500 μm;

[0075] (3) Set the spacing of the lattice units, specifically including the following situations:

[0076] 1) The spacing is greater than 0 mm and less than or equal to 0.1 mm;

[0077] 2) The spacing is greater than 0.1 mm and less than or equal to 2 mm;

[0078] 3) The spacing is greater than 2 mm and less than or equal to 5 mm;

[0079] 4) The spacing is greater than 5 mm;

[0080] (4) Set the three-dimensional and surface morphologies of the lattice units, including the following cases:

[0081] 1) All lattice units are in a convex morphology, and the surface morphology is set to a rounded corner or an acute angle;

[0082] 2) All lattice units are in a concave morphology, and the surface morphology is set to a rounded corner or an acute angle;

[0083] 3) The lattice units are alternately set in convex and concave morphologies, and the surface morphology is set to a rounded corner or an acute angle;

[0084] (5) Set the height or depth of the lattice units. When the lattice units are in a convex morphology, set their height. When the lattice units are in a concave morphology, set their depth.

[0085] S105. 3D print the designed outer layer of the core body.

[0086] S2. Set the outer layer of the core body prepared in S1 on the rotating shaft platform, align the nozzle of the extrusion and inkjet 3D printing device with the outer layer 9 of the core body. According to the actual printing shape, start the first motor 4 to change the angle between the outer layer 9 of the core body and the ground, or start the second motor 6 to rotate the outer layer 9 of the core body around its own axis, or start the first motor 4 and the second motor 6 simultaneously. Macroscopically, increase the printing trajectory to achieve multiple degrees of freedom for the outer layer 9 of the core body when printing the bionic scaffold. Microscopically, it also has a local strengthening effect, and thus can form diverse curved surface morphologies.

[0087] Furthermore, the outer layer 9 of the core body can be manufactured using various forming processes, including subtractive processes, machining, additive manufacturing processes, DLP stereolithography technology, LCD structured light forming technology, SLA, and SLM.

[0088] As Figure 2 shown, the rotating shaft platform includes a motor assembly, a shock-absorbing base plate 1, a rotating shaft mandrel 7, and a thermostat 8. The side wall of the shock-absorbing base plate 1 is fixedly connected to the motor assembly. The output end of the motor assembly is connected to the rotating shaft mandrel 7. The thermostat 8 is detachably connected inside the rotating shaft mandrel 7. The outer layer 9 of the core body is sleeved outside the rotating shaft mandrel 7; the outer layer 9 of the core body is connected to the rotating shaft mandrel 7 in an interference fit manner, or can also be connected by a buckle or a bolt, and the outer layer 9 of the core body and the rotating shaft mandrel 7 rotate synchronously.

[0089] The concentricity of the rotating shaft mandrel 7 is not less than 0.05 mm, which can ensure that when preparing the micro outer layer 9 of the core body, the appearance structure of the micro outer layer 9 of the core body can meet the design requirements and avoid deviation.

[0090] The motor assembly includes a first housing 2, a second housing 3, a first motor 4, a second motor 6 and a connecting sleeve 5. The first housing 2 is fixedly connected to the side wall of the shock-absorbing bottom plate 1. The first motor 4 is arranged inside the first housing 2. The first motor 4 is installed inside the first housing 2 through a motor base 12. The first housing 2 is bolted to the second housing 3 through the connecting sleeve 5. The connecting sleeve 5 is installed inside one end of the first housing 2 close to the second housing 3. The connecting sleeve 5 is rotatably connected to the first housing 2. The connecting sleeve 5 is sleeved on the outer wall of the output end of the first motor 4, and the connecting sleeve 5 rotates synchronously with the output end of the first motor 4. The second motor 6 is arranged inside the second housing 3. The output end of the second motor 6 is not on the same straight line as the output end of the first motor 4 and is not parallel, preferably perpendicular. The output end of the second motor 6 is connected to a rotating shaft mandrel 7. The first motor 4 is used to drive the second housing 3 to rotate, so as to drive the second motor 6 inside the second housing 3 to rotate around the axis of the output end of the first motor 4. The second motor 6 is used to drive the rotating shaft mandrel 7 to rotate. That is, the motor assembly can not only make the rotating shaft mandrel 7 rotate around its own axis, but also make the rotating shaft mandrel 7 rotate around the axis of the output end of the first motor 4, so as to increase the rotational freedom of the outer layer 9 of the core body.

[0091] The output end of the second motor 6 is located below the output end of the first motor 4, effectively avoiding the interference between the first motor 4 and the second motor 6.

[0092] The rotational speeds of the first motor 4 and the second motor 6 are set to 0 - 4500 r / min, the control accuracy is not less than ±0.1°, the rated voltage is set to 200 - 220V, the insulation class is 155F, the maximum torque is not less than 3.81 N·m, and the maximum axial load is not less than 209N, providing a certain load support and enabling automatic forward and reverse control. When the motor assembly runs at high speed, it is in a nano-level precision structure control mode, and when it runs at low speed, it is in a micro-level precision structure control mode.

[0093] The temperature controller 8 is used to control the temperature of the rotating shaft mandrel 7. The temperature controller 8 is provided with a first temperature controller and a first temperature controller. The first temperature controller is installed inside the rotating shaft mandrel 7 in the high-temperature temperature control mode for use, and the first temperature controller is installed inside the rotating shaft mandrel 7 in the low-temperature temperature control mode for use.

[0094] As Figure 3 shown, the first temperature controller adopts a single-head heating tube. The temperature control range of the first temperature controller is 25 - 500°C, and the temperature control accuracy is ±0.1°C, which can achieve good adsorption after the extrusion of high-temperature molten materials.

[0095] As Figure 4As shown in the figure, the second temperature controller includes a fastener 81, a refrigerant pipe 82, a refrigerant channel 83, a heat sink 84, and a superconducting column 85. The first temperature controller is snap-connected inside the rotating shaft mandrel 7 through the fastener 81. Fasteners 81 are snap-connected to both the upper and lower ends of the inner wall of the rotating shaft mandrel 7. One end of the refrigerant pipe 82 is installed on the fastener 81 located at the lower end of the rotating shaft mandrel 7, and the other end of the refrigerant pipe 82 is installed on the fastener 81 located at the upper end of the rotating shaft mandrel 7. The refrigerant pipe 82 passes through the fastener 81 located at the upper end of the rotating shaft mandrel 7. The U-shaped refrigerant channel 83 is provided inside the refrigerant pipe 82, and the refrigerant channel 83 communicates with the external refrigerant. The heat sink 84 is sleeved on the outer wall of the refrigerant pipe 82. The upper end of the heat sink 84 contacts the lower wall of the fastener 81 located at the upper end of the rotating shaft mandrel 7, and the lower end of the heat sink 84 contacts the upper wall of the fastener 81 located at the lower end of the rotating shaft mandrel 7. The superconducting column 85 is sleeved on the outer wall of the heat sink 84. The upper end of the superconducting column 85 is sleeved on the outer wall of the fastener 81 located at the upper end of the rotating shaft mandrel 7, and the lower end of the superconducting column 85 is sleeved on the outer wall of the fastener 81 located at the lower end of the rotating shaft mandrel 7; after the first temperature controller is installed inside the rotating shaft mandrel 7, the outer wall of the superconducting column 85 contacts the inner wall of the rotating shaft mandrel 7. Refrigerant is introduced into the refrigerant channel 83, and then through refrigerant conduction, low-temperature control of the rotating shaft mandrel 7 is achieved. The temperature control range is -40 to 25 °C; the temperature control accuracy is ±0.1 °C, which can achieve better shape maintenance after printing for cold-forming materials.

[0096] As Figure 5 shown in the figure, the motor assembly may further include a first housing 2, a second housing 3, a first motor 4, a second motor 6, a worm 10, and a worm gear 11. The first motor 4 is installed inside the first housing 2 through a motor base 12. The output end of the first motor 4 is fixedly connected to the worm 10. The worm gear 11 is fixedly connected to the second housing 3. The worm gear 11 is arranged inside the first housing 2, and the worm 10 meshes with the worm gear 11. By starting the first motor 4, the first motor 4 drives the worm 10 to rotate, thereby driving the worm gear 11 to rotate, the angle between the outer layer 9 of the core body and the ground is changed. In addition, the worm and worm gear meshing method in the above motor assembly can be replaced with a gear meshing method.

[0097] Furthermore, using the above method to design a bionic stent for an artificial blood vessel that needs surgical resection for some stenosis, by presetting the lattice unit type, size, spacing, height or depth, three-dimensional shape, and surface shape on the outer surface of the digital model, the outer layer of the core body of the artificial blood vessel is obtained, and then the outer layer of the core body is sleeved on the rotating shaft platform to form a corresponding bionic stent, as Figure 6 、 Figure 7 shown in the figure, the corresponding bionic stent of the artificial blood vessel forms a centripetal arc in the concave area. By designing multiple groups of centripetal arc-shaped structures, the centripetal contraction ability of the prepared bionic stent can be enhanced, and its mechanical support performance can be maintained.

[0098] Furthermore, using the above method to design the outer layer of the core of a transcatheter artificial heart valve, by constructing a spatial convex lattice unit structure on the outer surface of the digital model, the required outer layer of the core of the artificial heart valve is obtained, and then the corresponding bionic stent of the required artificial heart valve is obtained. As Figure 8 shown, the obtained bionic stent further improves the spatial void distribution, thereby enhancing the cell adhesion ability. At the same time, the risk of radial folding during valve release also needs to be considered, and the shape distribution on the valve surface is set to cope with the force situation of the valve.

[0099] Finally, it should be noted that the above content is only used to illustrate the technical solution of the present invention, rather than limiting the protection scope of the present invention. Any simple modification or equivalent replacement made by those of ordinary skill in the art to the technical solution of the present invention shall not depart from the essence and scope of the technical solution of the present invention.

Claims

1. A preparation method of a bionic scaffold with micro-nano structure regulation, characterized in that, It includes the following steps: S1. Prepare the outer layer of the core body corresponding to the bionic scaffold; specifically, it includes the following steps: S101. Obtain the image file data of the surface structure to be replaced; S102. Use medical reconstruction software to reconstruct the model of the image file obtained in step S101, and obtain the three-dimensional model information of the true surface curvature of the surface structure to be replaced; S103. Use triangular patch optimization software to perform noise removal, normal repair, and encapsulation operations on the three-dimensional model information obtained in step S102 to obtain a structural model; S104. Import the structural model obtained in S103 into reverse reconstruction software for the structural design of the outer layer of the core body; S105. Manufacture the designed outer layer of the core body; S2. Place the outer layer of the core body prepared in S1 on the rotating shaft platform, and use a 3D printing device and the rotating shaft platform to complete the preparation of the bionic scaffold corresponding to the surface structure to be replaced.

2. The preparation method of a bionic scaffold with micro-nano structure regulation according to claim 1, characterized in that, The structural model described in step S103 includes the surface structure to be replaced and its extended part structure; S104 specifically includes the following steps: S1041. In the reverse reconstruction software, perform fitting and design according to the surface curvature of the adjacent part of the surface structure to be replaced to obtain a digital model that matches the surface structure of the adjacent part of the surface structure to be replaced. This digital model is the macroscopic size of the outer layer of the core body; S1042. Design the texture structure of the outer layer of the core body by setting lattice units on the outer surface of the digital model.

3. The preparation method of a bionic scaffold with micro-nano structure regulation according to claim 2, wherein, S1042 is specifically represented as follows: (1) Set the type of lattice unit, including at least triangle, square, honeycomb, and rhombus; (2) Set the size of the lattice unit. It is set that all lattice units have an inscribed circle feature, and the size of the lattice unit is set by the diameter of the inscribed circle, including the following situations: 1) The diameter of the inscribed circle is greater than or equal to 0μm and less than 100μm; 2) The diameter of the inscribed circle is greater than or equal to 100μm and less than 500μm; 3) The diameter of the inscribed circle is greater than or equal to 500μm and less than 1000μm; 4) The diameter of the inscribed circle is greater than or equal to 1000μm and less than 1500μm; 5) The diameter of the inscribed circle is greater than or equal to 1500μm; (3) Set the spacing of the lattice units, specifically including the following situations: 1) The spacing is greater than 0mm and less than or equal to 0.1mm; 2) The spacing is greater than 0.1mm and less than or equal to 2mm; 3) The spacing is greater than 2mm and less than or equal to 5mm; 4) The spacing is greater than 5mm; (4) Set the three-dimensional form and surface form of the lattice unit, including the following situations: 1) All lattice units are in a convex form, and the surface form is set to a rounded corner or an acute angle; 2) All lattice units are in a concave form, and the surface form is set to a rounded corner or an acute angle; 3) The lattice units are alternately set in convex and concave forms, and the surface form is set to a rounded corner or an acute angle; (5) Set the height or depth of the lattice unit. When the lattice unit is in a convex form, set its height, and when the lattice unit is in a concave form, set its depth.

4. The preparation method of a bionic scaffold with micro-nano structure regulation according to claim 1, characterized in that, The rotating shaft platform includes a motor assembly and a rotating shaft mandrel. The motor assembly includes a first motor, a second housing, and a second motor. The output end of the first motor is connected to the second housing. The second motor is installed inside the second housing, and the output end of the second motor is connected to the rotating shaft mandrel. The outer wall of the rotating shaft mandrel is sleeved with an outer layer of the core.

5. The preparation method of a bionic scaffold with micro-nano structure regulation according to claim 4, characterized in that, A thermostat is installed inside the rotating shaft mandrel. The thermostat includes a first thermostat and a second thermostat. The first thermostat is installed inside the rotating shaft mandrel in the high-temperature temperature control mode, and the second thermostat is installed inside the rotating shaft mandrel in the low-temperature temperature control mode.

6. The preparation method of a bionic scaffold with micro-nano structure regulation according to claim 5, characterized in that, The first thermostat uses a single-head heating tube.

7. The preparation method of a bionic scaffold with micro-nano structure regulation according to claim 5, characterized in that The second thermostat includes a fastener, a refrigerant pipe, a heat sink, and a superconducting column. The end of the refrigerant pipe is installed on the fastener. A refrigerant channel is provided inside the refrigerant pipe. The heat sink is sleeved on the outer wall of the refrigerant pipe, and the superconducting column is sleeved outside the heat sink.

8. The preparation method of a bionic scaffold with micro-nano structure regulation according to claim 5, characterized in that, The temperature control range of the first thermostat is 25 - 500 °C; the temperature control range of the second thermostat is -40 - 25 °C; the temperature control accuracy of both the first thermostat and the second thermostat is ±0.1 °C.

9. The preparation method of a bionic scaffold with micro-nano structure regulation according to claim 4, characterized in that, S2 specifically includes the following steps: S201. Place the outer layer of the core outside the rotating shaft mandrel, and align the nozzle of the printing device with the outer layer of the core. S202. Start the first motor, and according to the specific external shape of the prepared bionic scaffold, change the angle between the outer layer of the core and the ground, or separately start the second motor to rotate the outer layer of the core around its own axis, or simultaneously start the second motor to rotate the outer layer of the core around its own axis.

10. The preparation method of a bionic scaffold with micro-nano structure regulation according to claim 1, characterized in that, The manufacturing and forming process of the outer layer of the core includes subtractive processes, machining, additive manufacturing processes, DLP stereolithography technology, LCD structured light forming technology, SLA, and SLM.

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