Sargassum pallidum polypeptide face cream and preparation method thereof

Abstract

The invention discloses sargassum pallidum polypeptide face cream and a preparation method thereof, and belongs to the technical field of cosmetics. The face cream is prepared by taking low-molecular-weight sargassum pallidum polypeptide as a core active component, optimizing the synergistic effect of an emulsification system and the polypeptide, combining components such as an oil phase, a water phase and a stabilizer, and performing melting, homogenizing and emulsifying processes. The polypeptide is small in molecular weight and high in transdermal absorption rate, has remarkable oxidation resistance, and can effectively scavenge free radicals and inhibit skin oxidative damage. The prepared face cream is fine and stable, easy to spread and excellent in sensory evaluation. A stability test proves that the product is not layered under heat-resistant, cold-resistant and centrifugal conditions, and the pH value meets the standard requirements of cosmetics. The preparation process is simple and controllable, has high-activity component permeability, formula stability and industrial production potential, and provides an innovative scheme for development of natural anti-oxidation face cream.

Description

Technical Field

[0001] The invention relates to the technical field of cosmetics and preparation methods thereof, and in particular to an artemisia seaweed polypeptide facial cream and a preparation method thereof. Background Art

[0002] In the field of skin care, the application of antioxidants is crucial to delaying oxidative damage to the skin. Traditional antioxidant creams mostly rely on vitamin derivatives or synthetic antioxidant ingredients. Although they have certain free radical scavenging capabilities, they generally have defects such as low bioavailability, insufficient photothermal stability, and poor transdermal absorption efficiency. Especially during long-term storage, such ingredients are prone to activity attenuation and are difficult to effectively penetrate the skin barrier, limiting their actual application effects.

[0003] In recent years, peptide ingredients from natural sources have attracted attention due to their high biocompatibility and low irritation. However, the molecular weight distribution range of conventional animal and plant-derived peptides is wide and their transdermal penetration efficiency is limited, making it difficult for them to form an effective concentration in the stratum corneum. Marine brown algae extracts have become a research hotspot due to their unique antioxidant activity, among which the peptide components contained in Sargassum pallidum have shown significant antioxidant potential. However, the direct application of Sargassum pallidum crude extracts in the prior art has obvious deficiencies: the molecular weight of the unpurified peptide components is relatively high, resulting in low transdermal efficiency; the conventional emulsification system has poor compatibility with the active ingredients, which can easily cause the stability of the active ingredients to deteriorate during storage, affecting the effectiveness of the product.

[0004] In addition, the existing cream formulation process mostly adopts a single variable optimization method, which makes it difficult to coordinate the synergistic effects among multiple components. When bioactive peptides are introduced, they are often accompanied by problems such as decreased stability of the emulsification system and sticky skin feel, which restricts the industrial application of highly active natural ingredients. Therefore, the development of cream preparations with high stability, good skin feel and significant antioxidant effects is still a technical bottleneck that needs to be broken through in this field. Summary of the invention

[0005] The technical problem to be solved by the present invention is to provide a sargassum seaweed polypeptide cream and a preparation method thereof in view of the deficiencies in the prior art.

[0006] In order to achieve the purpose of the present invention, the following technical means are specifically adopted:

[0007] A sargassum seaweed polypeptide facial cream, characterized by comprising the following components in percentage by mass:

[0008] Phase A: emulsifier and oil phase ingredients, including PEG-100 stearic acid 1%-3% and 5%-6%, cetearyl alcohol 2%-6%, myristic acid glyceryl 2%-4%, monostearate glyceryl 1%-3%, liquid paraffin 4%;

[0009] Phase B: Aqueous phase components, including 5% - 25% of an aqueous solution of Sargassum polypeptide with a concentration of 50 mg / mL, 10% - 30% glycerol, 0.5% butanediol, 0.2% - 1.2% xanthan gum, 1% disodium EDTA, and the balance of water is made up to 99.99%;

[0010] Phase C: 0.01% essence;

[0011] Among them, the molecular weight of the Sargassum polypeptide is ≤1000 Da, the purity is ≥99%, the loss on drying is ≤5.0%, the ash content is ≤5.0%, no pesticide residues and pathogenic bacteria are detected, and the total heavy metal content is ≤10 ppm.

[0012] The Sargassum polypeptide cream is characterized in that the mass percentages of the components in Phase A are: 3% PEG - 100 stearic acid, 6% cetearyl alcohol, 3% glyceryl myristate, and 3% glyceryl monostearate.

[0013] The Sargassum polypeptide cream is characterized in that the addition amount of xanthan gum in Phase B is 0.8%, and the mass percentage of glycerol is 15%.

[0014] The Sargassum polypeptide cream is characterized in that the pH value of the cream is 4.0 - 6.0, and there is no stratification under the conditions of heat resistance at 40°C, cold resistance at -24°C, and centrifugation at 3000 r / min.

[0015] The preparation method of the Sargassum polypeptide cream is characterized by including the following steps:

[0016] (1) Mix the components of Phase A, heat and melt them at 80 - 90°C to form a homogeneous oil phase;

[0017] (2) Mix the components of Phase B, heat and dissolve them at 80 - 90°C to form a homogeneous aqueous phase;

[0018] (3) Slowly add the aqueous phase to the oil phase under stirring conditions, and homogenize at 2000 - 5000 r / min for 5 - 15 minutes after mixing;

[0019] (4) After cooling to room temperature, add essence and stir evenly to obtain the finished product.

[0020] The preparation method is characterized in that the rotation speed of the homogenization step is 3000 r / min and the time is 10 minutes.

[0021] Application of the Sargassum polypeptide cream in the preparation of antioxidant cosmetics.

[0022] The Sargassum pallidum polypeptide described in the present invention is an off-white powder, with a particle size standard such that 100% can pass through an 80-mesh sieve, the peptide content (on a dry basis) ≥ 99%, the molecular weight ≤ 1000 Da, the drying loss ≤ 5.0%, the ash content ≤ 5.0%, no pesticide residues can be detected, the total heavy metal content ≤ 10 ppm, the arsenic content ≤ 10 ppm, the lead content ≤ 10 ppm, the total number of colonies ≤ 1000 cfu / g, the number of yeasts and molds ≤ 100 cfu / g, no Escherichia coli can be detected, no Salmonella can be detected, and no Staphylococcus can be detected.

[0023] Beneficial effects

[0024] The beneficial effects of the present invention are as follows:

[0025] 1. High-efficiency antioxidant and natural active ingredients. The antioxidant performance of the Sargassum pallidum polypeptide is verified by the ABTS free radical scavenging and hydroxyl free radical scavenging rates, and its molecular weight ≤ 1000 Da is easy to penetrate and absorb. The apple antioxidant experiment shows that compared with the blank matrix, this cream can effectively inhibit the browning caused by oxidation, delay skin oxidative damage, and brighten the skin tone.

[0026] 2. Scientific formula and stable industrial production. Through single-factor and orthogonal experiments to optimize the ratio, the paste is delicate and easy to spread, and the sensory score reaches more than 80 points; it passes the heat resistance, cold resistance, and centrifugal stability tests, the pH value meets the specifications, the process is simple and controllable, suitable for large-scale production, and has both safety and industrialization potential. Description of the drawings

[0027] Figure 1 It is the test result of the antioxidant performance of the cream. Specific implementation manners

[0028] The technical solution of the present invention will be clearly and completely described below in conjunction with the embodiments of the present invention. Obviously, the described embodiments are only a part of the embodiments of the present invention, rather than all of the embodiments. The description of at least one exemplary embodiment below is actually only illustrative and in no way restricts the present invention and its application or use. Based on the embodiments of the present invention, all other embodiments obtained by those of ordinary skill in the art without creative efforts belong to the scope of protection of the present invention.

[0029] Description of the Sargassum fusiforme polypeptide used in the present invention: The manufacturer is Xi'an Mugo Biotechnology Co., Ltd., the product batch number is MG-2024081502, the appearance is off-white powder, the particle size standard is that 100% can pass through an 80-mesh sieve, the peptide content (based on dry basis) is 99.07%, the molecular weight ≤ 1000 Da, the drying loss is 0.79%, the ash content is 1.42%, no pesticide residues are detected, the total heavy metal content ≤ 10 ppm, the arsenic content ≤ 10 ppm, the lead content ≤ 10 ppm, the total number of colonies ≤ 1000 cfu / g, the number of yeasts and molds ≤ 100 cfu / g, no Escherichia coli is detected, no Salmonella is detected, and no Staphylococcus is detected.

[0030] Example 1 Determination of the antioxidant activity of Sargassum fusiforme polypeptide

[0031] 1. Solution preparation

[0032] (1) Preparation of the mother solution of Sargassum fusiforme polypeptide

[0033] Take 250 mg of the Sargassum fusiforme polypeptide sample and make up the volume to 25 mL with distilled water to obtain a mother solution of 10.0 mg / mL.

[0034] (2) Preparation of the mother solution of glutathione

[0035] Weigh 250 mg of L-reduced glutathione, make up the volume to 25 mL with distilled water, mix well, and prepare a mother solution of 10.0 mg / mL.

[0036] (4) Preparation of PBS buffer solution

[0037] Weigh 1.36 g of potassium dihydrogen phosphate and 0.87 g of dipotassium hydrogen phosphate, dissolve them in 800 mL of deionized water, stir until completely dissolved, then finely adjust the pH to 6.8 ± 0.1 with 1M HCl or NaOH, make up the volume to 1 L with additional deionized water, add 8.0 g of NaCl and 0.2 g of KCl, stir to dissolve, and then autoclave (121 °C, 20 minutes) and store at 4 °C.

[0038] (5) Preparation of ABTS stock solution

[0039] Weigh 200.0 mg of ABTS and 34.4 mg of potassium persulfate, dissolve them in 50 mL of distilled water, shake well, and place in the dark at room temperature for 16 h to obtain the ABTS mother solution. Take an appropriate amount of the ABTS mother solution and dilute it with 95% ethanol to an absorbance value (measured by an ultraviolet spectrophotometer at a wavelength of 734 nm) of 0.70 ± 0.02 to obtain a solution, which is used as the ABTS assay solution and should be prepared freshly before use.

[0040] (6) 9 mmol / L ferrous sulfate solution

[0041] Weigh 250.2 mg of ferrous sulfate powder, and make up the volume to 100 mL with distilled water in a volumetric flask to obtain a 9 mmol / L ferrous sulfate solution.

[0042] (7) 9 mmol / L salicylic acid ethanol solution

[0043] Weigh 31.075 mg of salicylic acid powder, and make up the volume to 25 mL with 95% ethanol in a volumetric flask to obtain a 9 mmol / L salicylic acid ethanol solution.

[0044] 2. Determination of ABTS free radical scavenging rate

[0045] Determination was carried out according to the method of "Determination of Antioxidant Activity of Polypeptides - DPPH and ABTS Methods" (GB / T 39100 - 2020): First, dilute the Sargassum fusiforme polypeptide stock solution with 95% ethanol to a gradient solution of 2 - 10 mg / mL, and dilute the glutathione stock solution with 95% ethanol solution to a gradient solution of 0.02 - 1 mg / mL.

[0046] Add each reagent according to the combination in Table 1 and mix well by rapid oscillation. Add 3.6 mL of ABTS solution and 0.4 mL of glutathione solution with different concentrations to the samples in Group 1, and add 3.6 mL of ABTS solution and 0.4 mL of Sargassum fusiforme polypeptide solution with different concentrations to the samples in Group 2. The above two groups are used as the experimental groups (A s represents the absorbance value of this group); add 3.6 mL of ABTS solution and 0.4 mL of pure water solution to the samples in Group 3 as the blank group (A b represents the absorbance value of this group). After mixing well respectively, react in the dark at room temperature for 5 min, and measure the absorbance value with an ultraviolet spectrophotometer at a wavelength of 734 nm. ABTS free radical scavenging rate = (A b - A s ) / A b × 100%.

[0047] Table 1 Reagent addition conditions

[0048] Solution Name <![CDATA[1 set (A s )]]> <![CDATA[2 groups (A s )]]> <![CDATA[3 groups (A b )]]> ABTS Solution 3.6 mL 3.6 mL 3.6 mL Glutathione Solution 0.4 mL - - Sargassum Polypeptide Solution - 0.4 mL - Pure Water Solution - - 0.4 mL

[0049] The specific determination results are shown in Table 2 and Table 3.

[0050] Table 2 Absorbance determination results of glutathione group

[0051] Concentration (mg / mL) 0.02 0.04 0.06 0.08 0.2 0.4 0.6 0.8 1 Absorbance 0.24 0.147 0.11 0.085 0.046 0.016 0.009 0.008 0.007

[0052] Table 3 Absorbance determination results of Sargassum fusiforme polypeptide group

[0053] Concentration (mg / mL) 2 4 6 8 10 Absorbance 0.489 0.393 0.228 0.002 0.001

[0054] According to the above data, the half-clearance amount EC50 of glutathione is 0.019 mg / mL, and the half-clearance amount EC50 of Sargassum polypeptide is 4.2 mg / mL. The above results indicate that Sargassum polypeptide has the ability to scavenge ABTS free radicals.

[0055] 3. Determination of Hydroxyl Radical Scavenging Ability

[0056] The mother liquor of Sargassum polypeptide was diluted to prepare sample solutions with concentrations of 2, 4, 6, 8, and 10 mg / mL. Each reagent was added according to the combination in Table 4 below. Each sample solution was reacted in a water bath at 37°C for 30 min, and the absorbance was measured at 510 nm using a microplate reader and recorded as A1 (sample group), A2 (control group), and A3 (blank group). Calculate according to the following formula: Scavenging rate (%) = (1 - (A1 - A3) / A2) × 100%

[0057] Table 4 Reagent Addition Conditions

[0058] Solution Name Group A1 (Sample Group) Group A2 (Control Group) Group A3 (Blank Group) Sargassum Polypeptide Solution 50 μL - 50 μL 9 mmol / L Ferrous Sulfate Solution 50 μL 50 μL 50 μL 9 mmol / L Salicylic Acid Ethanol Solution 50 μL 50 μL 50 μL <![CDATA[9mmol / L H2O2]]> 50 μL 50 μL - Purified Water - 50 μL 50 μL

[0059] The specific measurement results are shown in Table 5 below.

[0060] Table 5 Determination Results of Scavenging Rates of Sample Solutions with Different Concentrations

[0061] Concentration (mg / mL) 2 4 6 8 10 Clearance Rate (%) -1.33 2.47 18.92 36.75 59.53

[0062] The above results indicate that Sargassum polypeptide has the ability to scavenge hydroxyl radicals.

[0063] Example 2 Development of Sargassum Polypeptide Cream

[0064] 1. Considering the properties of the active ingredients and the general composition of the cream, the following initial prescription was formulated.

[0065] Table 6 Initial Prescription of Sargassum Polypeptide Cream

[0066]

[0067] Note: The sum of each component in the formula is 100%, allowing a deviation of ±0.05% due to weighing accuracy.

[0068] 2. The following preliminary preparation method for this product was formulated:

[0069] (1) Weigh all the raw materials in phase A and place them in a clean beaker 1. Heat and melt them in a constant temperature water bath at 85°C, and keep them warm in the water bath for use as the oil phase;

[0070] (2) Weigh all the raw materials in phase B and place them in a clean beaker 2. Heat and melt them in a constant temperature water bath at 85°C;

[0071] (3) Under the condition of 85 °C water bath, while stirring, slowly add the raw materials of phase B into phase A. After stirring evenly, homogenize at 3000 rpm for 10 min;

[0072] (4) Place the homogenized mixture of phases A and B at room temperature, add the essence of phase C, and stir evenly to obtain the O / W type cream.

[0073] 3. The calculation and measurement methods of some evaluation indexes are as follows:

[0074] 3.1 Sensory evaluation

[0075] Randomly select 10 volunteers with healthy skin to conduct sensory evaluation on the cream in the same environment. Take appearance, spreadability, moisturizing degree, and stability as the inspection indexes. Appearance: The paste should be delicate, uniform, and without visible coarse particles to the naked eye. Spreadability: After smearing ten circles, whether it is easy to spread on the skin during the smearing process. Moisturizing degree: The texture is fresh and not greasy. Stability: Centrifuge at 6000 r / min for 15 min, and check whether there is layering of the cream. The specific judgment criteria are shown in the following table.

[0076] Table 7 Judgment criteria for sensory test

[0077]

[0078] 4. Experimental investigation

[0079] 4.1 Single-factor investigation

[0080] 4.1.1 Single-factor investigation on the prescription dosage of PEG-100 stearic acid

[0081] Set different prescription addition amounts of PEG-100 stearic acid (1%, 2%, 3%, 4%, 5%, 6%). Refer to Table 6 for the usage amounts of other materials. Conduct single-factor experiments, and conduct sensory evaluation on the creams with different contents of PEG-100 stearic acid added above to determine the optimal addition amount of PEG-100 stearic acid. The experimental results are shown in Table 8 below.

[0082] Table 8 Investigation results of the addition amount of PEG-100 stearic acid

[0083] Addition Amount (%) Appearance Score Spreading Property Score Moisturizing Degree Score Stability Score Total Score 1 21.25 19.75 21.25 25 87.25 2 19.5 18.75 18.75 25 82 3 19 17.5 19.5 25 81 4 16.5 15 17.75 25 74.25 5 22.5 20.25 21.5 25 89.25 6 20 20 18 25 83

[0084] As can be seen from the above table, the sensory scores of the creams prepared when the prescription addition amount of PEG-100 stearic acid is 1-3% and 5-6% are both good (>80 points). Therefore, the prescription addition amount of PEG-100 stearic acid of 1-3% and 5-6% can both be used as the qualified addition amounts of this product. At the same time, it can be seen that the sensory score is the highest when the addition amount is 5%. Therefore, temporarily select the prescription addition amount of PEG-100 stearic acid of 5% for subsequent experimental investigation.

[0085] 4.1.2 Univariate Investigation of the Prescribed Dosage of Cetearyl Alcohol

[0086] According to the above investigation, the addition amount of PEG - 100 Stearic Acid was set at 5%, and the addition amounts of cetearyl alcohol in the prescription (1%, 2%, 3%, 4%, 5%, 6%) were investigated. The amounts of other ingredients in the prescription are shown in Table 6. A univariate investigation experiment was conducted on the facial cream, and the sensory evaluation of the cream bodies with different added amounts of cetearyl alcohol was carried out to determine the optimal addition amount of cetearyl alcohol. The experimental results are shown in Table 9 below.

[0087] Table 9 Investigation Results of the Addition Amount of Cetearyl Alcohol

[0088] Addition Amount (%) Appearance Score Spreading Property Score Moisturizing Degree Score Stability Score Total Score 1 16.67 16.67 16.67 25 75.01 2 19.33 19.33 19.67 25 83.33 3 19 20.33 19.67 25 84 4 23 22.33 22 25 92.33 5 24.33 23.33 22.67 25 95.33 6 22.67 21.33 20.33 25 89.34

[0089] As can be seen from the above table, when the addition amount of cetearyl alcohol in the prescription is 2 - 6%, the sensory scores of the prepared cream bodies are all good (>80 points). Therefore, the addition amount of cetearyl alcohol in the prescription of 2 - 6% can all be used as the qualified addition amount of this product. At the same time, it can be seen that when the addition amount is 5%, the sensory score is the highest. Therefore, the addition amount of cetearyl alcohol in the prescription of 5% is temporarily selected for subsequent experimental investigation.

[0090] 4.1.3 Univariate Investigation of the Prescribed Dosage of Glyceryl Myristate

[0091] According to the above investigation, the addition amounts of PEG - 100 Stearic Acid and cetearyl alcohol were both set at 5%, and the addition amounts of glyceryl myristate in the prescription (2%, 3%, 4%, 5%, 6%) were investigated. The amounts of other ingredients in the prescription are shown in Table 6. A univariate investigation experiment was conducted on the facial cream, and the sensory evaluation of the cream bodies with different added amounts of glyceryl myristate was carried out to determine the optimal addition amount of glyceryl myristate. The experimental results are shown in Table 10 below.

[0092] Table 10 Investigation Results of the Addition Amount of Glyceryl Myristate

[0093] Addition Amount (%) Appearance Score Spreading Property Score Moisturizing Degree Score Stability Score Total Score 2 21.75 20 20.5 25 87.25 3 22.5 21.5 21.75 25 90.75 4 20.75 20.75 21 25 87.5 5 15.25 15.75 16.25 25 72.25 6 16 16.75 16.75 25 74.5

[0094] As can be seen from the above table, when the addition amount of glyceryl myristate in the prescription is 2 - 4%, the sensory scores of the prepared cream bodies are all good (>80 points). Therefore, the addition amount of glyceryl myristate in the prescription of 2 - 4% can all be used as the qualified addition amount of this product. At the same time, it can be seen that when the addition amount is 3%, the sensory score is the highest. Therefore, the addition amount of glyceryl myristate in the prescription of 3% is temporarily selected for subsequent experimental investigation.

[0095] 4.1.4 Univariate Investigation of the Prescribed Dosage of Glyceryl Monostearate

[0096] According to the above investigation, the addition amounts of PEG-100 stearic acid and cetearyl alcohol were both set at 5%, the addition amount of glyceryl myristate in the formulation was set at 3%, and the addition amounts of glyceryl monostearate in the formulation (1%, 2%, 3%, 4%, 5%) were investigated. The dosages of other ingredients in the formulation are shown in Table 6. A single-factor investigation experiment was carried out on the facial cream, and the sensory evaluation of the cream bodies with different added amounts of glyceryl monostearate was carried out to determine the optimal addition amount of glyceryl monostearate. The experimental results are shown in Table 11 below.

[0097] Table 11 Investigation results of the addition amount of glyceryl monostearate

[0098] Addition Amount (%) Appearance Score Spreading Property Score Moisturizing Degree Score Stability Score Total Score 1 22 18 20.67 25 85.67 2 22 18.67 21.67 25 87.34 3 19.33 17.67 20.33 25 82.33 4 17.67 17.67 18.33 25 78.67 5 15.33 16.67 17 25 74

[0099] As can be seen from the above table, the sensory scores of the cream bodies prepared when the addition amount of glyceryl monostearate in the formulation is 1-3% are all good (>80 points). Therefore, the addition amount of glyceryl monostearate in the formulation of 1-3% can all be used as the qualified addition amount of this product. At the same time, it can be seen that the sensory score is the highest when the addition amount is 2%. Therefore, the addition amount of glyceryl monostearate in the formulation of 2% is temporarily selected for subsequent experimental investigation.

[0100] 4.1.5 Single-factor investigation of the prescription dosage of glycerol

[0101] According to the above investigation, the addition amounts of PEG-100 stearic acid and cetearyl alcohol were both set at 5%, the addition amount of glyceryl myristate in the formulation was set at 3%, the addition amount of glyceryl monostearate in the formulation was set at 2%, and the addition amounts of glycerol in the formulation (10%, 15%, 20%, 25%, 30%) were investigated. The dosages of other ingredients in the formulation are shown in Table 6. A single-factor investigation experiment was carried out on the facial cream, and the sensory evaluation of the cream bodies with different added amounts of glycerol was carried out to determine the optimal addition amount of glycerol. The experimental results are shown in Table 12 below.

[0102] Table 12 Investigation results of the addition amount of glycerol

[0103] Addition Amount (%) Appearance Score Spreading Property Score Moisturizing Degree Score Stability Score Total Score 10 22.67 21.67 23.33 25 92.67 15 21.33 21.67 23 25 91 20 22.33 20 22 25 89.33 25 21.67 19.33 19.67 25 85.67 30 20.67 18.33 18.33 25 82.33

[0104] As can be seen from the above table, the sensory scores of the cream bodies prepared when the addition amount of glycerol in the formulation is 10-30% are all good (>80 points). Therefore, the addition amount of glycerol in the formulation of 10-30% can all be used as the qualified addition amount of this product. At the same time, it can be seen that the sensory score is the highest when the addition amount is 10%. Therefore, the addition amount of glycerol in the formulation of 10% is temporarily selected for subsequent experimental investigation.

[0105] 4.1.6 Single-factor investigation of the prescription dosage of xanthan gum

[0106] Based on the above investigation, the addition amounts of PEG-100 stearic acid and cetearyl alcohol were both set at 5%, the addition amount of glyceryl myristate in the prescription was set at 3%, the addition amount of glyceryl monostearate in the prescription was set at 2%, and the addition amount of glycerol in the prescription was set at 10%. The addition amounts of xanthan gum in the prescription (0.2%, 0.5%, 0.8%, 1.0%, 1.2%) were investigated. The dosages of other ingredients in the prescription are shown in Table 6. A single-factor investigation experiment was carried out on the cream, and the sensory evaluation was carried out on the cream bodies with different xanthan gum contents added above to determine the optimal addition amount of xanthan gum. The experimental results are shown in Table 13 below.

[0107] Table 13 Investigation Results of Xanthan Gum Addition Amount

[0108] Addition Amount (%) Appearance Score Spreading Property Score Moisturizing Degree Score Stability Score Total Score 0.2 18.67 22 23.33 25 89 0.5 21.33 21.33 23 25 90.66 0.8 22.67 21.67 23.33 25 92.67 1.0 23.33 20 20 25 88.33 1.2 24 19.67 19.67 25 88.34

[0109] As can be seen from the above table, the sensory scores of the cream bodies prepared when the addition amount of xanthan gum in the prescription is 0.2 - 1.2% are all good (>80 points). Therefore, the addition amount of xanthan gum in the prescription of 0.2 - 1.2% can all be used as the qualified addition amount of this product. At the same time, it can be known that the sensory score is the highest when the addition amount is 0.8%. Therefore, the addition amount of xanthan gum in the prescription of 0.8% is temporarily selected for subsequent experimental investigation.

[0110] 4.2 Orthogonal Experiment Investigation

[0111] Based on the results of the single-factor experiment, five factors that have a relatively significant impact on the comprehensive sensory score were selected: PEG-100 stearic acid, cetearyl alcohol, glyceryl myristate, glyceryl monostearate, and glycerol. An orthogonal experiment with five factors and four levels was designed for investigation to determine the optimal cream formulation. The specific orthogonal experiment factor level table is shown in Table 14 below.

[0112] Table 14 Orthogonal Experiment Factor Levels

[0113]

[0114] According to the above design table, the cream was prepared and evaluated. The specific results are shown in Table 15.

[0115] Table 15 Orthogonal Investigation Results

[0116]

[0117]

[0118] From the above investigation results, it can be known that the order of the factors affecting the sensory score of the cream is: myristic acid > PEG-100 stearic acid > cetearyl alcohol > glycerol > glyceryl monostearate; the optimal preparation process is PEG-100 stearic acid 3%, cetearyl alcohol 6%, glyceryl myristate 3%, glyceryl monostearate 3%, and glycerol 15%.

[0119] 4.3 Investigation on the Prescription Addition Amount of 50 mg / mL Sargassum polypeptide aqueous solution

[0120] Set the addition amount of PEG - 100 stearic acid in the prescription as 3%, the addition amount of cetearyl alcohol as 6%, the addition amount of glyceryl myristate as 3%, the addition amount of glycerol monostearate as 3%, and the addition amount of glycerol as 15%. Investigate the prescription addition amount (5%, 10%, 15%, 20%, 25%) of 50 mg / mL Sargassum polypeptide aqueous solution. The dosage of other components in the prescription is shown in Table 6. Conduct a single - factor investigation experiment on the cream, and conduct a sensory evaluation on the cream bodies with the above different addition amounts to determine the optimal addition amount of 50 mg / mL Sargassum polypeptide aqueous solution. The experimental results are shown in Table 16 below.

[0121] Table 16 Investigation Results of the Addition Amount of 50 mg / mL Sargassum polypeptide aqueous solution

[0122] Addition Amount (%) Appearance Score Spreading Property Score Moisturizing Degree Score Stability Score Total Score 5 20.67 21 22 25 88.67 10 22 21.33 23 25 91.33 15 22.33 20 22 25 89.33 20 22 18.67 21.67 25 87.34 25 22 19.67 19.67 25 86.34

[0123] As can be seen from the above table, when the prescription addition amount of 50 mg / mL Sargassum polypeptide aqueous solution is 5 - 25%, the sensory scores of the prepared cream bodies are all good (>80 points). Therefore, the prescription addition amount of 50 mg / mL Sargassum polypeptide aqueous solution of 5 - 25% can all be used as the qualified addition amount of this product. At the same time, it can be known that the sensory score is the highest when the addition amount is 10%.

[0124] Combined with the above investigation results, the prescription composition of Sargassum polypeptide cream is determined as follows:

[0125]

[0126] Note: The prescription addition amount range of PEG - 100 stearic acid is 1 - 3% and 5 - 6%, the prescription addition amount range of cetearyl alcohol is 2 - 6%, the prescription addition amount range of glyceryl myristate is 2 - 4%, the prescription addition amount range of glycerol monostearate is 1 - 3%, the prescription addition amount range of glycerol is 10 - 30%, the prescription addition amount range of xanthan gum is 0.2 - 1.2%, and the prescription addition amount range of 50 mg / mL Sargassum polypeptide aqueous solution is 5 - 25%. The sum of each component in the formula is 100%, allowing a deviation of ±0.05% due to weighing accuracy.

[0127] Evaluation of Sargassum polypeptide cream in Example 3

[0128] 1. Sensory evaluation

[0129] Prepare Sargassum polypeptide cream according to the prescription screened by the above steps. The paste is delicate, uniform, without visible coarse particles to the naked eye, easy to spread on the skin, and the texture is fresh and not greasy.

[0130] 2. pH test

[0131] According to the requirements of the "Technical Specifications for Cosmetics", the pH value of the cream should be between 4.0 and 8.0. The dilution method was used for detection with reference to the national standard of the People's Republic of China GB / T 13531.1-2008. Specific method: Weigh 2 g of the cream (accurate to 0.1 g), add 20 mL of test water that has been boiled and cooled, heat to 40 °C, stir continuously, and cool to room temperature for standby. Measure the pH value of the above sample with a pH meter. The pH values of 5 samples measured are 5.06, 5.03, 5.00, 4.99, and 4.94 in sequence, indicating that the pH of this product meets the requirements of the "Technical Specifications for Cosmetics".

[0132] 3. Stability evaluation

[0133] 3.1 Heat resistance investigation

[0134] This product is an O / W type cream. Referring to the O / W type detection standard, set the temperature of the constant temperature incubator to 40 °C. Take 1.5 g of the sample and transfer it into a 2 ml EP tube, place it in the constant temperature incubator, and take it out after 24 h to observe whether there is any layering phenomenon of the moisturizing cream. Result: There is no layering phenomenon, indicating that the heat resistance of this product is qualified.

[0135] 3.2 Cold resistance investigation

[0136] Set the refrigerator temperature to -24 °C. Take 1.5 g of the sample and transfer it into a 2 ml EP tube, place it in the refrigerator, and take it out after 24 h to observe whether there is any layering phenomenon of the moisturizing cream. Result: There is no layering phenomenon, indicating that the cold resistance of this product is qualified.

[0137] 3.3 Centrifugal stability investigation

[0138] Take 1.5 g of the sample and transfer it into a 2 ml EP tube, centrifuge at 3000 r / min for 30 min, and observe whether there is any layering phenomenon of the cream. Result: There is no layering phenomenon, indicating that the centrifugal stability of this product is qualified.

[0139] 4. Antioxidant performance test of the cream

[0140] Four groups were set for the antioxidant test of the Sargassum fusiforme polypeptide cream. The test method is as follows: Take 4 newly cut apples (each piece is 1 cm × 1 cm × 1 cm), apply 0.1 g of the sample cream, 0.1 g of the commercially available cream, and 0.1 g of the cream matrix without adding the Sargassum fusiforme polypeptide extract on the surfaces of 3 of them respectively, and use the remaining 1 piece for the blank control experiment. Observe the color change of the cut surface of each apple after standing at room temperature for 10 hours. The experimental results are shown in Figure 1 . It can be seen from the results that the cream has good antioxidant performance compared with the blank control group.

[0141] The above are only the preferred embodiments of the present invention and are not intended to limit the present invention. Any modifications, equivalent replacements, improvements, etc. made within the spirit and principles of the present invention shall be included within the protection scope of the present invention.

Claims

1. A sargassum seaweed polypeptide cream, characterized in that: Contains the following components in percentage by weight: Phase A: emulsifier and oil phase ingredients, including PEG-100 stearic acid 1%-3% and 5%-6%, cetearyl alcohol 2%-6%, myristic acid glyceryl 2%-4%, monostearate glyceryl 1%-3%, liquid paraffin 4%; Phase B: aqueous phase components, including 5% to 25% of 50 mg / mL sargassum polypeptide aqueous solution, 10% to 30% glycerol, 0.5% butylene glycol, 0.2% to 1.2% xanthan gum, 1% disodium EDTA, and the balance is made up to 99.99% with water; Phase C: flavor 0.01%; The molecular weight of the sea wormwood polypeptide is ≤1000Da, the purity is ≥99%, the loss on drying is ≤5.0%, the ash content is ≤5.0%, pesticide residues and pathogenic bacteria are not detected, and the total amount of heavy metals is ≤10ppm.

2. The sargassum seaweed polypeptide cream according to claim 1, characterized in that: The mass percentages of the components of phase A are: 3% PEG-100 stearic acid, 6% cetearyl alcohol, 3% glyceryl myristic acid, and 3% glyceryl monostearate.

3. The sargassum seaweed polypeptide cream according to claim 1, characterized in that: The added amount of xanthan gum in the phase B is 0.8%, and the mass percentage of the glycerol is 15%.

4. The sargassum seaweed polypeptide cream according to claim 1, characterized in that: The pH value of the facial cream is 4.0-6.0, and there is no stratification under the conditions of heat resistance at 40°C, cold resistance at -24°C and centrifugation at 3000 r / min.

5. A method for preparing the Artemisia seaweed polypeptide cream according to any one of claims 1 to 4, characterized in that: The following steps are involved: (1) Mix the components of phase A and heat to melt at 80-90° C. to form a uniform oil phase; (2) Mix the components of phase B and heat to dissolve at 80-90°C to form a uniform aqueous phase; (3) Slowly add the water phase to the oil phase under stirring conditions, and after mixing, homogenize at 2000-5000 r / min for 5-15 minutes; (4) After cooling to room temperature, add essence and stir evenly to obtain the finished product.

6. The preparation method according to claim 5, characterized in that: The speed of the homogenization step is 3000 r / min and the time is 10 minutes.

7. Use of the Sargassum seaweed polypeptide cream as claimed in claim 1 in the preparation of antioxidant cosmetics.

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