Soothing composition and soothing product and their application and cosmetics

Abstract

This invention belongs to the field of daily chemicals and discloses a soothing composition obtained by water extraction using Inonotus obliquus, radix dichotoma, and rye seeds as raw materials. The weight ratio of Inonotus obliquus, radix dichotoma, and rye seeds is 1-3:5-15:2-5. This composition, using Inonotus obliquus, radix dichotoma, and rye seeds as raw materials, can produce a product with a good soothing effect through water extraction. Further research has found that the soothing effect is even more pronounced when commercially available rye seed extract is added to the above composition. The invention also discloses the soothing product, its application, and cosmetics.

Description

Technical Field

[0001] The present invention relates to the field of daily chemicals, and in particular to a soothing composition and a soothing product, applications thereof, and cosmetics. Background Art

[0002] Soothing skin care products mainly relieve redness and sensitivity, repair the skin barrier, moisturize and calm the skin, and have anti-inflammatory and antibacterial effects. Their core function is to help sensitive or damaged skin return to a stable state.

[0003] The patent application with publication number CN113398050A is about a mite-removing and oil-controlling shower gel. It discloses that the extract of Inonotus obliquus has strong oil-control, antioxidant, anti-inflammatory and allergy-reducing effects. Its anti-allergic Chinese medicine composition is composed of Lithospermum erythrorhizon extract, Saposhnikovia divaricata extract, Polygonatum odoratum extract, Angelica sinensis extract and Licorice extract.

[0004] In Traditional Chinese Medicine, fangfeng (Saposhnikovia divaricata) is known for its ability to reduce fever, relieve pain, and regulate the immune system. Modern research has shown that it can reduce fever, relieve headaches and joint pain, reduce skin redness and swelling caused by allergies, enhance the body's resistance, and help improve blood viscosity and inhibit abnormal cell growth.

[0005] Previous studies in this application demonstrated that the combination of Saposhnikovia divaricata and Inonotus obliquus has a significant soothing effect. However, the soothing effect of these two substances is more pronounced in terms of histamine inhibition in IgE-induced RBL-2H3 cells, while their effectiveness in inhibiting hyaluronidase, which is strongly correlated with histamine release from mast cells, needs improvement. This suggests that the enhanced soothing effect is determined by multiple pathways. Summary of the Invention

[0006] The object of the present invention is to provide a soothing composition, which uses Inonotus obliquus, Parsnip root, and rye seeds as raw materials, and can obtain a product with good soothing effect through water extraction; in further research of the present invention, it was found that when commercially available rye seed extract was additionally added to the above-mentioned composition, its soothing effect was more obvious.

[0007] At the same time, the invention also discloses the application of the composition and the product, as well as cosmetics.

[0008] To achieve the above objectives, this application discloses:

[0009] A soothing composition is obtained by water extraction using Inonotus obliquus, Parsnip root and rye seeds as raw materials;

[0010] The weight ratio of the Inonotus obliquus, the radix psilocybin and the rye seeds is 1-3:5-15:2-5.

[0011] The characteristics of the raw materials in the soothing composition of the present invention are:

[0012] Inonotus obliquus: The active ingredients in Inonotus obliquus (such as triterpenoids) can inhibit the release of inflammatory factors and reduce inflammatory reactions such as tissue redness and swelling;

[0013] Windproof: Reduces skin redness and swelling caused by allergies and enhances body resistance;

[0014] Rye seeds: Extracts containing arabinoxylan can be obtained through water extraction. Arabinoxylan is mentioned in some cosmetic raw materials as having anti-aging functions. In the research of this invention, it was found that when it is used alone, it does not have obvious soothing ability. When it is used in combination with siler and inonotus obliquus, the soothing ability of the formula is significantly enhanced. This result may be related to the antioxidant function of arabinoxylan, which can relieve inflammatory responses through multiple mechanisms.

[0015] The common mechanism of action of the above three is:

[0016] Existing literature reports that Inonotus obliquus contains abundant inonotol, a polyphenolic active substance that can inhibit IKKβ phosphorylation, block NF-κB nuclear translocation, and reduce TNF-α and IL-6 release. Chromones in the root of Saposhnikovia root inhibit IκBα degradation, enhancing the NF-κB inhibitory effect. In the present invention, experimental results can infer that the mechanism of action of the two is: through the combined action of the two, dual blocking NF-κB activation and reducing the expression of inflammatory factors. Furthermore, the Saposhnikovia root extract can inhibit the degranulation of IgE-sensitized RBL-2H3 cells and reduce the level of inflammatory mediators. At the same time, the triterpenoid compounds in Inonotus obliquus can also activate Nrf2, promote the expression of HO-1 and SOD, and scavenge ROS. The phenolic acids in rye seeds can directly neutralize free radicals, thereby synergistically enhancing the antioxidant effect.

[0017] In the soothing composition, the water extraction process is as follows: crushing Inonotus obliquus, radix psilocybin and rye seeds, adding water, extracting, concentrating and precipitating with alcohol, and concentrating the filtrate after filtering.

[0018] In the above-mentioned soothing composition, the water extraction process is: crush the raw materials and pass them through a 40-mesh sieve. The raw materials are Inonotus obliquus, radix silosus, and rye seeds. Add 10 times the volume of water of the crushed raw materials, stir and extract at 70-90°C, and centrifuge to remove the residue. The filtrate is concentrated, precipitated with alcohol, and ethanol is removed. Then, polyols, preservatives, water, or other fermentation product filtrate are added.

[0019] In the above soothing composition, taking the total weight of the soothing composition as 100%, the weight of the raw materials is equivalent to 10-20% of the total weight of the soothing composition.

[0020] At the same time, the present invention also discloses a soothing product, comprising any of the soothing compositions described above and a rye seed extract; the rye seed extract is equivalent to 0.05 to 0.2 wt% of the total weight of the soothing composition; the rye seed extract is produced by Silab, France, and the active ingredient content in the rye seed extract is 5.50 wt%.

[0021] Further research in the present invention found that the additional addition of rye seed extract can significantly improve the soothing ability of the product. The possible reason is that the raw material is composed of arabinoxylan taken from rye seeds, which blocks downstream MyD88 signaling by binding to TLR2 / 4 receptors, inhibiting the activation of the IKK complex, thereby reducing IκBα degradation and NF-κB nuclear translocation. It reduces the expression of pro-inflammatory factors, while inhibiting ERK and JNK phosphorylation, reducing COX-2 and PGE2 production, and thus alleviating inflammatory responses. By additionally adding rye seed arabinoxylan to the product, the problem of polysaccharide loss during the product purification process is solved, the active ingredient content index is improved, and it is more effective in inhibiting the expression of inflammatory factors, thereby achieving a soothing effect.

[0022] In addition, the present invention also discloses the use of any of the above soothing compositions or the above soothing products in preparing cosmetics.

[0023] Finally, the present invention also discloses a cosmetic comprising 0.1 to 30 wt % of any one of the above soothing compositions or the above soothing products.

[0024] This application has at least the following beneficial effects:

[0025] The composition of the present invention uses Inonotus obliquus, radix psidium, and rye seeds as raw materials, and after water extraction, a product with good soothing effect can be obtained; in further research of the present invention, it was found that when commercially available rye seed extract was additionally added to the above-mentioned composition, the soothing effect was even more obvious. BRIEF DESCRIPTION OF THE DRAWINGS

[0026] Figure 1 Photo of the chicken chorioallantoic membrane test for sample 7. DETAILED DESCRIPTION

[0027] Below in conjunction with embodiments of the present invention, the present invention is clearly and completely described, in description of the present invention, it should be noted that, in the embodiment, the unrecited specific conditions person, carry out according to the condition of normal condition or manufacturer's suggestion. Reagents therefor or instrument are not recited manufacturer person, are the conventional products that can be obtained by commercial purchase. When not making special instructions, used part in the embodiments of the present invention is all weight part.

[0028] Source of raw materials

[0029] Inonotus obliquus, silanol, and rye seeds were purchased from a Chinese medicine store. The moisture content of Inonotus obliquus was less than 13%, the moisture content of silanol was less than 10%, and the moisture content of rye seeds was less than 8%.

[0030] The supplier of rye seed extract is Silab, France; the active ingredient is rye (SECALE CEREALE) seed extract, and the active ingredient content is 5.50 wt%.

[0031] Preparation of the first part of the composition

[0032] Inonotus obliquus, radix dichotoma, and rye seeds were ground to a total weight of 15 g, with the proportions shown in Table 1. Ten times the volume of water was added, and the mixture was stirred and extracted at 80°C for 3 h. The residue was removed by centrifugation, and the filtrate was concentrated at 65°C to 1 / 3 of the original extract volume. An equal volume of 95% ethanol was added to the concentrate for precipitation. The precipitate was centrifuged and concentrated to an alcohol-free state. A polyol and water were added to a total weight of 100 g, with the polyol accounting for 30% of the total weight of the polyol and water. Ultrafiltration was performed to obtain an ultrafiltrate, and a preservative (0.05% octanoylhydroxamic acid, 0.08% ethylhexylglycerin, and 0.6% 1,2-hexanediol) was added to the ultrafiltrate, followed by sterilization to obtain the finished product.

[0033] The formula table can be found in Table 1;

[0034] Table 1 Formula (Unit: weight ratio)

[0035] Inonotus obliquus Parsnip root rye seeds Sample 1 1 15 2 Sample 2 1.5 10 3.5 Sample 3 3 5 5 Sample 4 2 12 4 Sample 5 2.5 8 3 Comparison sample 1 1.5 10 0 Comparison sample 2 1.5 0 3.5 Comparison sample 3 0 10 3.5 Comparison sample 4 0 0 3.5

[0036] Part 2 Preparation of Soothing Products

[0037] During the process of adding the preservative to the soothing composition of the first part, rye seed extract (the amount thereof is shown in Table 2) is added simultaneously, and the mixture is sterilized to obtain the finished product.

[0038] Table 2 Recipe

[0039]

[0040] Part III Performance Test

[0041] 3.1 Inflammatory factor IL-8 inhibition test

[0042] Macrophages can be used as a cell model to study the ability of cosmetics to inhibit the inflammatory factor IL-8. By stimulating with bacterial lipopolysaccharide (LPS), the inflammatory factor IL-8 levels in the negative control and test sample groups are measured to evaluate the effectiveness of the test samples in suppressing IL-8. IL-8 levels are determined using an enzyme-linked immunosorbent assay (ELISA).

[0043] This test refers to the laboratory method and compares the results of cellular IL-8 inflammatory factor content determination between the test sample and the negative control. If the cellular IL-8 inflammatory factor content of the test sample is lower than that of the negative control, it can be considered that the test sample has a certain soothing effect.

[0044] This test method is an in vitro method that provides a method for detecting the content of IL-8 inflammatory factor in macrophages, which can be used as one of the evidences to support the soothing efficacy claims of cosmetic raw materials and finished products.

[0045] Experimental materials and methods

[0046] Instruments: RT-6100 ELISA analyzer;

[0047] Cells used in the experiments: macrophages (RAW264.7 cells);

[0048] Test method:

[0049] (1) Treatment of test samples

[0050] Sample group: The sample was diluted with pure water to a 4% solution, then filtered with a 0.22 μm filter, and the filtrate was collected as the sample mother solution;

[0051] Negative control: basal culture medium (cell activity test); basal culture medium containing LPS (IL-8 inflammatory factor content determination).

[0052] (2) Test operation steps

[0053] Cell activity test: RAW264.7 cells were plated in 96-well plates. After 24 hours, the culture medium was aspirated and the basal culture medium containing different concentrations of the test samples was added. After 24 hours, the OD490nm was detected by MTT method, and the effect of the test samples on the activity of RAW264.7 cells was calculated.

[0054] IL-8 inflammatory factor content determination: RAW264.7 cells were plated onto 96-well plates and cultured for 24 hours. The culture medium was then replaced with basal medium containing different concentrations of the test sample. After 24 hours, the cell supernatant was collected and centrifuged. The supernatant was aspirated and the IL-8 content in the cell culture supernatant was measured using an ELISA kit to determine the effect of the test sample on IL-8 content in macrophages.

[0055] Calculation formula

[0056] .

[0057] The test results refer to Table 3;

[0058] 3.2 Histamine inhibition rate test

[0059] RBL-2H3 cells can be used as a cell model to study the histamine inhibition of cosmetics. By inducing IgE in RBL-2H3 cells, histamine levels in the negative control and test sample groups are measured to evaluate the effectiveness of the test sample in inhibiting histamine levels. Histamine levels are determined using an enzyme-linked immunosorbent assay (ELISA).

[0060] This test refers to the laboratory method and compares the cellular histamine content determination results of the test sample with that of the negative control. If the cellular histamine content of the test sample is lower than that of the negative control, it can be considered that the test sample has a certain soothing effect.

[0061] This test method is an in vitro method that provides a method for detecting the histamine content in RBL-2H3 cells. It can be used as one of the evidences to support the soothing efficacy claims of cosmetic raw materials and finished products.

[0062] Experimental materials and methods

[0063] Instruments: RT-6100 ELISA analyzer;

[0064] Cells used in the experiments: rat basophils (RBL-2H3 cells);

[0065] Test method:

[0066] (1) Treatment of test samples

[0067] Sample group: The sample was diluted with pure water to a 4% solution by volume, then filtered through a 0.22 μm filter, and the filtrate was collected as the sample mother solution;

[0068] Negative control: basal culture medium (cell activity test); basal culture medium containing inducer (histamine content determination).

[0069] (2) Test operation steps

[0070] Cell activity test: RBL-2H3 cells were plated in 96-well plates. After 24 hours, the culture medium was discarded and basal culture medium containing different concentrations of test samples was added. After 24 hours, OD490nm was detected by MTT method, and the effect of the test samples on RBL-2H3 cell activity was calculated.

[0071] Histamine Content Assay: RBL-2H3 cells were plated onto 96-well plates and cultured for 24 hours. The culture medium was then replaced with basal medium containing various concentrations of the test sample. After 24 hours, the cell supernatant was collected and centrifuged. The supernatant was aspirated and the histamine content in the cell culture supernatant was measured using an ELISA kit to determine the effect of the test sample on the histamine content of RBL-2H3 cells.

[0072] (3) Calculation formula

[0073] .

[0074] The test results refer to Table 3;

[0075] 3.3 Hyaluronidase inhibition rate test

[0076] Hyaluronidase is a specific enzyme that cleaves hyaluronic acid and is a participant in allergic reactions, strongly correlated with the release of histamine from mast cells. The soothing effect of a test sample can be determined by its hyaluronidase inhibition rate. A higher hyaluronidase inhibition rate indicates a stronger soothing effect, and vice versa.

[0077] In this test, the hyaluronidase inhibition rate test results of the test sample and the negative control are compared. If the inhibition rate of the test sample is higher than that of the negative control, the test sample can be considered to have a soothing effect.

[0078] This test method is an in vitro method suitable for evaluating cosmetic products that claim to achieve soothing effects by inhibiting hyaluronidase.

[0079] Experimental materials and methods

[0080] Instruments: BSA224S analytical balance, L6s UV spectrophotometer;

[0081] Reagents: BR grade hyaluronidase, BR grade sodium hyaluronate.

[0082] Test method:

[0083] (1) Treatment of control and test samples

[0084] Sample group: diluted with pure water to a sample concentration of 5%;

[0085] Positive control (dipotassium glycyrrhizate, purity ≥98%): Dilute with water to a positive control concentration of 3%;

[0086] Negative control: pure water.

[0087] (2) Test operation steps

[0088] Set up sample group, sample background group, solvent group and solvent background group. Each group needs to set up 3 parallels. Add different reagent solutions to the four groups respectively, shake well, and place at room temperature for 30 minutes for color development. Use ultraviolet spectrophotometer to measure the absorbance value at a wavelength of 528 nm.

[0089] (3) Calculation formula

[0090] ;

[0091] Where: A is the absorbance of the reaction solution without sample and containing enzyme; B is the absorbance of the reaction solution without sample and without enzyme;

[0092] C—absorbance of the reaction solution containing sample and enzyme; D—absorbance of the reaction solution containing sample and no enzyme.

[0093] The test results refer to Table 3;

[0094] Table 3 Results

[0095] Group Inflammatory factor inhibition rate / % Histamine inhibition rate / % Hyaluronidase inhibition rate / % Sample 1 18.77 23.52 42.35 Sample 2 15.83 24.89 44.57 Sample 3 16.95 22.17 46.78 Sample 4 14.17 26.89 40.35 Sample 5 13.03 21.56 39.91 Sample 6 20.33 31.48 61.64 Sample 7 25.85 34.86 65.41 Sample 8 28.92 30.59 61.20 Comparison sample 1 11.62 15.19 32.15 Comparison sample 2 10.25 11.74 57.87 Comparison sample 3 8.37 9.80 14.19 Comparison sample 4 3.09 4.22 5.76 Negative control / / -0.53 Positive control / / 68.34

[0096] Result analysis:

[0097] 1. Comparative samples 1 to 3 show that Inonotus obliquus is the main factor in improving the hyaluronidase inhibition rate. The other two properties do not show obvious differences. Comparative sample 4 shows that rye seeds alone do not show obvious effects after extraction.

[0098] In samples 1 to 5, the contribution of the amount of Inonotus obliquus to the improvement of the hyaluronidase inhibition rate was not reflected, but the performance of samples 1 to 5 in the above three tests was generally better than that of control samples 1 to 4;

[0099] Among samples 1 to 5, sample 1 has the best inflammatory factor inhibition rate, and sample 4 has the best histamine inhibition rate. The content of radix fangfeng in sample 1 is the highest, and the content of rye seeds in sample 4 is the highest. Combined with the results of comparative samples 1 to 4, it can be roughly inferred that radix fangfeng and rye seeds play a more important role in the inhibition rate of inflammatory factors, and rye seeds play a more important role in the histamine inhibition rate. However, it should be noted that, combined with the data of comparative example 4, the histamine inhibition performance of rye seeds used alone is poor, and the simultaneous use of rye seeds and inonotus obliquus is more necessary. In addition, the results of comparative sample 1 suggest that the combination of inonotus obliquus and radix fangfeng also has a good histamine inhibition effect. From the perspective of sample 1 and sample 2, when inonotus obliquus and radix fangfeng are relatively more, the histamine inhibition ability is relatively high, which can also reflect the histamine inhibition ability of the two from one side. Since the gap between the samples is not large, the above conclusion does not rule out the existence of detection error.

[0100] The above results show that the performance of the product obtained by water extraction within the formulation range of the present invention is relatively balanced and excellent.

[0101] 2. It can be seen from samples 6 to 8 that the soothing performance is further enhanced after adding rye seeds. The possible reasons are that, on the one hand, there are material differences between commercially available products and self-extracted products. On the other hand, by additionally adding rye seed arabinoxylan to the product, the problem of polysaccharide loss during the purification process of the product is solved, and the active ingredient content index is improved, making it more effective in inhibiting the expression of inflammatory factors, thereby achieving a soothing effect.

[0102] Sample 7 of the present invention was subjected to a chicken embryo chorioallantoic membrane test, and the test results are shown in Tables 4 and 5. Figure 1 ;

[0103] Table 4 Test results

[0104] Serial number Sample name Sample processing method Test results (ES) 1 Negative control (9% saline) / 0.00 2 Positive control (1% SDS) / 18.00 3 Shuboyin The sample was diluted into a 2% aqueous solution 2.67

[0105] Application Examples

[0106] A soothing product is prepared according to the formula in Table 5. The preparation method is as follows:

[0107] Step 1: Dissolve phase B in advance and stir to mix evenly; mix phase C in advance and stir to mix evenly, set aside.

[0108] Step 2: Add the pure water from Phase A to the pot and start homogenizing. While homogenizing, add the remaining ingredients from Phase A one by one and homogenize at medium speed for 30-60 seconds. Heat to 80-85°C with stirring and homogenize at medium speed for 1-2 minutes until no gel particles remain. Keep warm for 30 minutes, then stir and cool.

[0109] Step 3: Cool down to 50℃, add pre-made phases B and C in sequence, and stir well.

[0110] Step 4: Cool to 45°C, add phase D and stir well.

[0111] Table 5 Basic soothing product formula

[0112]

Claims

1. A soothing composition, characterized in that It is obtained by water extraction using Inonotus obliquus, Parsnip root and rye seeds as raw materials; The weight ratio of the Inonotus obliquus, the radix dichotoma, and the rye seeds is 1-3:5-15:2-5. The water extraction process comprises the following steps: crushing the raw materials, the Inonotus obliquus, the radix dichotoma, and the rye seeds, and passing them through a 40-mesh sieve; adding water 10 times the volume of the crushed raw materials; stirring and extracting at 70-90° C.; centrifuging to remove the residue; concentrating the filtrate, precipitating with alcohol, removing ethanol, and then adding a polyol, a preservative, and water; Taking the total weight of the soothing composition as 100%, the weight of the raw materials is equivalent to 10-20% of the total weight of the soothing composition.

2. A soothing product, characterized in that The invention comprises the soothing composition according to claim 1 and rye seed extract; the rye seed extract is equivalent to 0.05-0.2 wt% of the total weight of the soothing composition; the rye seed extract is produced by Silab, France, and the active ingredient content in the rye seed extract is 5.50 wt%.

3. Use of the soothing composition according to claim 1 in preparing cosmetics.

4. A cosmetic, characterized in that: Contains 0.1 to 30 wt % of the soothing composition according to claim 1.

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