Preparation method of cuprous selenide nano-enzyme and photo-thermal application of cuprous selenide nano-enzyme
By using protective gas and specific additives in secondary distilled water, combined with sonication and stability treatment, cuprous selenide nanoenzymes with uniform particle size and consistent morphology were prepared, which solved the problems of complex preparation process, high cost and poor photothermal performance in the prior art, and achieved efficient and safe preparation of nanoenzymes and excellent photothermal performance.
Patent Information
- Application Number
- CN202510413726.6
- Authority / Receiving Office
- CN · China
- Patent Type
- Applications(China)
- Current Assignee / Owner
- Filing Date
- 2025-04-03
- Publication Date
- 2025-07-01
AI Technical Summary
The existing preparation methods for cuprous selenide nanoenzymes have problems such as environmental pollution, potential threats to human health, complex and time-consuming processes and high costs, and it is difficult to accurately control the consistency of particle size and morphology, affecting the photothermal performance of the material.
A kind of copper selenide nanoenzyme with uniform particle size and uniform morphology is prepared by mixing and uniform particle size and uniform morphology.
The safe and efficient preparation of cuprous selenide nanoenzymes is achieved, and its photothermal characteristics and stability are optimized, the photothermal conversion efficiency and catalytic activity are improved, and environmental pollution and preparation costs are reduced.
Smart Images
Figure CN120229691A_ABST
Abstract
Description
Technical Field
[0001] The present invention relates to the technical field of nano - material preparation, and more specifically, to a preparation method of cuprous selenide nanozyme and its application in photothermal aspects. Background Art
[0002] Cuprous selenide (Cu 2-x Se), as a material with special thermoelectric properties and rich reserves, has become a highly potential photothermal conversion material due to its complex crystal structure, ultra - low lattice thermal conductivity, and excellent photothermal properties; it not only exhibits excellent electrical and thermal properties, but also shows outstanding performance in efficient light absorption and photothermal conversion efficiency, opening up new ways for solutions to new photothermal therapy technologies; and with the development of nanotechnology, researchers have found that the performance of cuprous selenide can be significantly improved by controlling its particle size and morphology; nanoscale cuprous selenide not only inherits the excellent properties of traditional cuprous selenide, but also shows unique advantages in antioxidant properties, biocompatibility, and stability.
[0003] However, the existing preparation methods of cuprous selenide nanozyme, including solvothermal method, high - energy ball milling combined with spark plasma sintering, and techniques using corrosive solvents such as oleylamine and octadecene, face problems such as environmental pollution, potential threats to human health, complex and time - consuming processes, and high costs; in addition, these methods often have difficulty in precisely controlling the consistency of particle size and morphology, which has a direct impact on the photothermal properties of the material and severely limits its photothermal performance and application potential; therefore, it is particularly important to explore a preparation method of cuprous selenide nanozyme that can be produced safely and efficiently and can precisely regulate the uniformity of particle size and morphology.
[0004] In view of the problems in the related art, no effective solution has been proposed yet. Summary of the Invention
[0005] In view of the problems in the related art, the present invention provides a preparation method of cuprous selenide nanozyme and its application in photothermal aspects to overcome the above - mentioned technical problems existing in the related art.
[0006] Specifically, the technical solution adopted by the present invention is as follows: According to one aspect of the present invention, a preparation method of cuprous selenide nanozyme is provided, and the method includes: S1. Introduce a protective gas into double - distilled water, and add an additive and an adjuvant respectively and stir to obtain a selenide solution and a dispersion solution; S2. Perform stability treatment on the dispersion solution to obtain a stable dispersion solution, and perform ultrasonic treatment on the selenide solution to obtain a dispersed selenide solution; S3. Add the dispersed selenium solution to the stable dispersion solution for mixing and separation, and take the supernatant for purification and solidification to obtain cuprous selenide nanozyme.
[0007] Further, the additive includes: selenium source and copper source; the auxiliary agent includes: reducing agent and complexing agent.
[0008] Further, introduce a protective gas into the secondary distilled water, and add the additive and the auxiliary agent respectively and stir to obtain the selenium solution and the dispersion solution, including: Introduce a protective gas into the secondary distilled water, and add the selenium source and the reducing agent in sequence and stir to obtain the selenium solution; Introduce a protective gas into the secondary distilled water, and add the copper source and the complexing agent in sequence and stir to obtain the dispersion solution.
[0009] Further, the protective gas includes at least one of nitrogen or argon; the selenium source includes at least one of sodium selenite, selenium dioxide or selenium powder; the reducing agent includes at least one of sodium borohydride, ascorbic acid or hydrazine hydrate; the copper source includes at least one of copper chloride, copper sulfate or copper nitrate in different hydrated states; the complexing agent includes at least one of polyvinylpyrrolidone, ethylenediaminetetraacetic acid or sodium citrate.
[0010] Further, the reaction temperature of the selenium solution is 25 - 60 °C, the stirring speed is 200 - 500 rpm, and the stirring time is 30 - 120 minutes; the reaction temperature of the dispersion solution is 25 - 80 °C, the stirring speed is 300 - 600 rpm, and the stirring time is 20 - 60 minutes.
[0011] Further, perform stability treatment on the dispersion solution to obtain a stable dispersion solution, including: adjusting the pH value of the dispersion solution with a buffer solution, and heating the adjusted dispersion solution to obtain a stable dispersion solution.
[0012] Further, the buffer solution includes at least one of phosphate buffer solution, borate buffer solution or carbonate buffer solution; the heating method for heating the adjusted dispersion solution includes at least one of water bath heating, oil bath heating or heating magnetic stirrer, and the heating temperature is 50 - 80 °C.
[0013] Further, the frequency of ultrasonic treatment on the selenium solution is 20 - 40 kHz, and the time is 10 - 30 minutes.
[0014] Further, adding the dispersed selenium solution to the stable dispersion solution for mixing and separation, and taking the supernatant for purification and solidification to obtain cuprous selenide nanozyme, including: Add the dispersed selenium solution to the stable dispersion solution, and stir and mix to obtain a mixed solution; Centrifuge the mixed solution, let the centrifuged mixed solution stand, and extract the supernatant of the mixed solution after standing. Purify the supernatant to obtain purified cuprous selenide nanozyme, and solidify the purified cuprous selenide nanozyme to obtain cuprous selenide nanozyme; wherein, the purification includes at least one of ultrafiltration or dialysis; the solidification includes at least one of freeze-drying or vacuum-drying.
[0015] According to another aspect of the present invention, there is provided an application of cuprous selenide nanozyme in photothermal aspects.
[0016] The beneficial effects of the present invention are as follows: 1. By ultrasonic treatment, adjusting the pH value, reaction temperature, and using complexing agents, etc., the present invention helps to better control the particle size and morphology, obtain cuprous selenide nanozymes with uniform particle size and consistent morphology, thereby optimizing the photothermal properties and stability of cuprous selenide nanozymes. Its performance is superior to the products prepared by traditional methods, which not only helps to improve the photothermal conversion efficiency of cuprous selenide nanozymes, but also can optimize its catalytic activity and enhance its application potential in biomedicine.
[0017] 2. The present invention uses secondary distilled water as a solvent and prevents oxidation by introducing protective gases (such as nitrogen or argon), reducing the use of harmful chemicals and the risk of environmental pollution; at the same time, it also avoids the use of corrosive and toxic solvents, improves the preparation safety, and avoids long-term high-temperature and high-pressure treatment, simplifies the operation process, reduces the preparation cost, makes the preparation more efficient, and is more suitable for large-scale production. Brief Description of the Drawings
[0018] In order to more clearly illustrate the technical solutions in the embodiments of the present invention or the prior art, the following will briefly introduce the drawings required to be used in the embodiments. Obviously, the drawings in the following description are only some embodiments of the present invention. For those of ordinary skill in the art, without creative efforts, other drawings can also be obtained based on these drawings.
[0019] Figure 1 It is a schematic flow chart of a preparation method of cuprous selenide nanozyme according to an embodiment of the present invention. Detailed Embodiments
[0020] To further illustrate the embodiments, the present invention provides drawings. These drawings are part of the disclosure of the present invention, mainly used to illustrate the embodiments, and can be used in conjunction with the relevant descriptions in the specification to explain the operating principles of the embodiments. With reference to these contents, those of ordinary skill in the art should be able to understand other possible implementation manners and the advantages of the present invention. The components in the drawings are not drawn to scale, and similar component symbols are usually used to represent similar components.
[0021] According to an embodiment of the present invention, a preparation method of cuprous selenide nanozyme and its application in photothermal aspects are provided.
[0022] The present invention will be further described below in conjunction with the accompanying drawings and specific embodiments. As Figure 1 shown, the preparation method of cuprous selenide nanozyme according to an embodiment of the present invention includes: S1. Introduce a protective gas into secondary distilled water, and add an additive and an auxiliary agent respectively and stir to obtain a selenium solution and a dispersion solution.
[0023] Specifically, both the selenium solution and the dispersion solution are obtained in a three-necked round-bottom flask.
[0024] Specifically, the additive includes: a selenium source and a copper source; the auxiliary agent includes: a reducing agent and a complexing agent.
[0025] Specifically, introducing a protective gas into secondary distilled water, and adding an additive and an auxiliary agent respectively and stirring to obtain a selenium solution and a dispersion solution includes: Introduce a protective gas into secondary distilled water, and sequentially add a selenium source and a reducing agent and stir to obtain a selenium solution; Introduce a protective gas into secondary distilled water, and sequentially add a copper source and a complexing agent and stir to obtain a dispersion solution.
[0026] Specifically, the selenium solution is usually generated by the reaction of a selenium source and a reducing agent, and the solution may contain selenium nanoparticles or selenide ions (Se²⁻).
[0027] Specifically, the main function of introducing nitrogen is to provide an inert atmosphere protection, prevent oxidation, and improve the stability of the reaction.
[0028] Specifically, the protective gas includes at least one of nitrogen or argon; the selenium source includes at least one of sodium selenite, selenium dioxide or selenium powder; the reducing agent includes at least one of sodium borohydride, ascorbic acid or hydrazine hydrate; the copper source includes at least one of copper chloride, copper sulfate or copper nitrate in different hydration states; the complexing agent includes at least one of polyvinylpyrrolidone, ethylenediaminetetraacetic acid or sodium citrate.
[0029] Specifically, the reaction temperature of the selenium solution is 25-60°C, the stirring speed is 200-500 rpm, and the stirring time is 30-120 minutes; the reaction temperature of the dispersion solution is 25-80°C, the stirring speed is 300-600 rpm, and the stirring time is 20-60 minutes.
[0030] Specifically, to control the reduction degree of the selenium source, it is necessary to adjust the dosage of the reducing agent and the reaction time to ensure that the selenium source is fully reduced to active selenium species (such as Se²⁻ or Se nanoparticles), but to avoid excessive reduction leading to selenium aggregation.
[0031] For example, add 50 mL of secondary distilled water into a 100 mL three-necked round-bottom flask, introduce nitrogen for 10 minutes to remove the oxygen in the secondary distilled water, and then add selenium powder (39.48 mg, 0.5 mmol) and sodium borohydride (56.7 mg, 1.5 mmol) in sequence. Stir for 45 minutes under a nitrogen atmosphere until the solution becomes colorless to obtain a selenium solution; at the same time, add 25 mL of secondary distilled water into a 100 mL three-necked round-bottom flask, and then add CuCl2·2H2O (85 mg, 0.5 mmol) and PVP (200 mg) in sequence. Stir for 20 minutes under a nitrogen atmosphere to obtain a dispersion solution.
[0032] Specifically, polyvinylpyrrolidone (PVP) can adsorb on the surface of CuCl2 particles through its long-chain structure to prevent particle aggregation and improve dispersibility; the carbonyl oxygen atoms of PVP can coordinate with Cu²⁺ to form a stable complex; PVP can regulate the morphology and size of CuCl2 particles by selectively adsorbing on different crystal planes; at the same time, since PVP is soluble in water and various organic solvents, it can improve the solubility and uniformity of the reaction system.
[0033] Among them, by optimizing the concentration of the complexing agent and adjusting the concentration of PVP or other complexing agents, it is ensured that copper ions (Cu²⁺) are fully coordinated and stabilized to prevent copper ions from aggregating in subsequent reactions.
[0034] S2. Perform stability treatment on the dispersion solution to obtain a stable dispersion solution, and perform ultrasonic treatment on the selenium solution to obtain a dispersed selenium solution.
[0035] Specifically, performing stability treatment on the dispersion solution to obtain a stable dispersion solution includes: adjusting the pH value of the dispersion solution using a buffer solution and heating the adjusted dispersion solution to obtain a stable dispersion solution.
[0036] Specifically, the buffer solution includes at least one of phosphate buffer solution, borate buffer solution or carbonate buffer solution; the heating method for heating the adjusted dispersion solution includes at least one of water bath heating, oil bath heating or heating magnetic stirrer, and the heating temperature is 50~80 °C.
[0037] Specifically, the pH adjustment is to adjust the pH value of the dispersion solution through a buffer solution to make it within the range suitable for the formation of cuprous selenide (usually weakly alkaline).
[0038] Specifically, by heating the adjusted dispersion solution to 50 - 80 °C, the reaction activity can be enhanced while avoiding the decomposition of the complexing agent or the aggregation of copper ions due to excessive temperature.
[0039] Specifically, after the pH adjustment is completed, the temperature control is carried out; among them, the adjusted dispersion solution is placed in a water bath, an oil bath or a heating magnetic stirrer, and slowly heated to an appropriate temperature while maintaining a constant temperature and continuous stirring to ensure uniform heating of the solution; to improve the reaction activity and promote the formation of cuprous selenide; to avoid the decomposition of the complexing agent or the aggregation of copper ions due to excessive temperature.
[0040] Specifically, the frequency of ultrasonic treatment of the selenization solution is 20 - 40 kHz, and the time is 10 - 30 minutes.
[0041] Specifically, by ultrasonic treatment of the selenization solution, the possible aggregation of selenium nanoparticles can be broken, ensuring the uniform dispersion of selenium species.
[0042] Specifically, by ultrasonic treatment of the selenization solution, the ultrasonic equipment includes an ultrasonic cleaner or a probe - type ultrasonic instrument, and the frequency is usually 20 - 40 kHz; a glass or plastic container resistant to ultrasound (such as a beaker, a centrifuge tube) is selected to put the selenization solution into the ultrasonic equipment for ultrasonic treatment.
[0043] Specifically, the ultrasonic treatment steps include: solution transfer, ultrasonic parameter setting, dispersion treatment, and observation and adjustment.
[0044] Among them, solution transfer is to transfer the selenization solution into the ultrasonic container to ensure that the liquid level height is suitable for ultrasonic treatment (usually one - third to two - thirds of the container volume); ultrasonic parameter setting includes power setting, that is, according to the solution volume and the performance of the ultrasonic equipment, an appropriate power is set (usually 100 - 500 W), the ultrasonic frequency is set (usually 20 - 40 kHz), the ultrasonic time is set, that is, according to the solution properties and the target, the ultrasonic time is set (usually 10 - 30 minutes), and the temperature control is to adjust the solution to a suitable temperature; dispersion treatment is to start the ultrasonic equipment to make ultrasonic waves act on the selenization solution. During the ultrasonic process, the cavitation effect generated by ultrasonic waves will break the aggregation of selenium nanoparticles and promote the uniform dispersion of selenium species; observation and adjustment is to observe the changes in the solution (such as color, transparency). If there is still aggregation in the solution, the ultrasonic time can be appropriately extended or the power can be increased; the operation after ultrasonic treatment is to let the solution after ultrasonic treatment stand for a while, observe whether precipitation or stratification occurs. If there are large - particle impurities in the solution, they can be removed by filtration with a filter membrane or low - speed centrifugation, and the treated selenization solution is sealed and stored to avoid light and oxidation.
[0045] By ultrasonic treatment, the dispersibility and stability of the selenization solution can be significantly improved, laying a foundation for subsequent reactions (such as the synthesis of cuprous selenide nanozyme).
[0046] S3. Add the dispersed selenium solution to the stable dispersion solution for mixing and separation, and take the supernatant for purification and solidification to obtain cuprous selenide nanozyme.
[0047] Specifically, adding the dispersed selenium solution to the stable dispersion solution for mixing and separation, and taking the supernatant for purification and solidification to obtain cuprous selenide nanozyme includes: Add the dispersed selenium solution to the stable dispersion solution and stir to mix to obtain a mixed solution; Centrifuge the mixed solution and let the centrifuged mixed solution stand, then extract the supernatant of the standing mixed solution; Purify the supernatant to obtain purified cuprous selenide nanozyme, and solidify the purified cuprous selenide nanozyme to obtain cuprous selenide nanozyme; wherein, the purification includes at least one of ultrafiltration or dialysis; the solidification includes at least one of freeze-drying or vacuum-drying.
[0048] Specifically, stirring can ensure the uniform dispersion of cuprous selenide nanozyme and the full reaction contact between the dispersed selenium solution and the stable dispersion solution.
[0049] Specifically, centrifugation can be used to separate different components (such as solid particles and liquid) in the mixed solution; purification can be used to separate and purify cuprous selenide nanozyme, removing unreacted substances, solvents or other impurities.
[0050] Specifically, solidification can be used to remove the solvent, convert the product from liquid state to solid state, which is convenient for storage and use.
[0051] Specifically, adding the dispersed selenium solution to the stable dispersion solution means slowly dropping the dispersed selenium solution into the stable dispersion solution while quickly stirring to ensure the uniform contact between selenium species and copper ions, and avoid agglomeration caused by too high local concentration.
[0052] Specifically, during the process of adding the dispersed selenium solution to the stable dispersion solution and stirring to mix, a protective gas such as nitrogen or argon is needed to prevent the oxidation of cuprous selenide and improve the purity and stability of the product.
[0053] Specifically, ultraviolet-visible spectroscopy (UV-Vis) or dynamic light scattering (DLS) can be used to monitor the reaction process in real time to ensure the controllability of the size and morphology of cuprous selenide nanozyme.
[0054] Specifically, the reaction temperature during stirring and mixing is 60-70 °C; the stirring speed is 400-500 rpm; the stirring time is 90-120 minutes.
[0055] Specifically, before centrifuging the mixed solution, it needs to be left standing at an appropriate temperature for a period of time (such as 30 - 60 minutes) to allow the copper selenide nanoparticles to fully grow and stabilize.
[0056] Specifically, for the centrifugation of the mixed solution, the centrifugation speed is 3000 - 10000 rpm; the centrifugation time is 10 - 20 minutes.
[0057] Specifically, centrifugation can remove large particle aggregates by low - speed centrifugation and retain the supernatant of the nanoparticles with uniform dispersion.
[0058] Specifically, after centrifuging the mixed solution, let it stand, and extract the supernatant of the mixed solution after standing. The mixed solution after standing is the solution obtained by allowing the mixed solution after centrifugation to stand.
[0059] Specifically, purification is to remove the unreacted selenium source, copper source, reducing agent and complexing agent to improve the purity of the product.
[0060] Specifically, the supernatant is purified by ultrafiltration. The pressure of ultrafiltration is 0.1 - 0.5 MPa, and the ultrafiltration time is 30 - 60 minutes.
[0061] For example, use a 30 kDa ultrafiltration tube to ultrafilter several times to remove excess PVP.
[0062] Specifically, the supernatant is purified by dialysis for 24 - 48 hours, and the water is changed every 4 - 6 hours.
[0063] Specifically, further treatment is required before freeze - drying. Add an appropriate amount of protective agent (such as sucrose or trehalose) to the purified copper selenide nanozyme to prevent the nanoparticles from aggregating during drying; and use liquid nitrogen or a low - temperature refrigerator to quickly freeze the purified copper selenide nanozyme to avoid damage to the nanoparticle structure caused by ice crystal growth.
[0064] Specifically, the purified copper selenide nanozyme is solidified by freeze - drying. The freeze - drying temperature is - 50°C to - 80°C, and the time is 24 - 48 hours.
[0065] For example, place the purified copper selenide nanozyme in a 20 mL sample bottle and freeze - dry for 24 hours.
[0066] Specifically, the purified copper selenide nanozyme is solidified by vacuum drying. The vacuum drying temperature is 40 - 60°C, and the time is 12 - 24 hours.
[0067] According to another embodiment of the present invention, an application of copper selenide nanozyme in photothermal aspects is provided.
[0068] In summary, by means of the above technical solutions of the present invention, through ultrasonic treatment, adjusting the pH value, reaction temperature, and using complexing agents, etc., it helps to better control the particle size and morphology, obtain cuprous selenide nanozymes with uniform particle size and consistent morphology, thereby optimizing the photothermal properties and stability of cuprous selenide nanozymes. Their performance is superior to that of products prepared by traditional methods, which not only helps to improve the photothermal conversion efficiency of cuprous selenide nanozymes, but also optimizes their catalytic activity and enhances their application potential in biomedicine; in addition, the present invention uses secondary distilled water as a solvent and prevents oxidation by introducing a protective gas (such as nitrogen or argon), reducing the use of harmful chemicals and the risk of environmental pollution; at the same time, it also avoids the use of corrosive and toxic solvents, improves the preparation safety, and avoids long-term high-temperature and high-pressure treatment, simplifies the operation process, reduces the preparation cost, makes the preparation more efficient, and is more suitable for large-scale production.
[0069] Mild Phototherapy is a treatment method using low-energy and non-invasive light waves. Its core lies in stimulating human tissues or cells with light of specific wavelengths and intensities to achieve multiple effects such as anti-inflammatory, repair, and immune regulation.
[0070] The wavelength of Cu2-XSe@AHI was determined by ultraviolet absorption, and it was found that there was infrared absorption at about 1064 nm. By exciting Cu2-XSe@AHI with 1064 nm, heat can be generated to treat the kidneys of mice.
[0071] The above are only the preferred embodiments of the present invention and are not intended to limit the present invention. Any modifications, equivalent replacements, improvements, etc. made within the spirit and principle of the present invention shall be included in the protection scope of the present invention.
Claims
1. A method for preparing cuprous selenide nanozyme, characterized in that: The method includes: S1. Passing protective gas into double distilled water, and adding additives and auxiliary agents respectively for stirring to obtain a selenized solution and a dispersed solution; S2, performing stability treatment on the dispersed solution to obtain a stable dispersed solution, and performing ultrasonic treatment on the selenized solution to obtain a dispersed selenized solution; S3. Add the dispersed selenide solution to the stable dispersed solution for mixing and separation, and take the supernatant for purification and solidification to obtain cuprous selenide nanozyme.
2. The method for preparing a cuprous selenide nanozyme according to claim 1, characterized in that: The additives include: a selenium source and a copper source; The auxiliary agent includes: a reducing agent and a complexing agent.
3. The method for preparing a cuprous selenide nanozyme according to claim 2, characterized in that: The step of introducing a protective gas into the double distilled water, and adding an additive and an auxiliary agent respectively for stirring to obtain a selenized solution and a dispersed solution comprises: A protective gas is introduced into double distilled water, and a selenium source and a reducing agent are added in sequence and stirred to obtain a selenized solution; A protective gas is introduced into the double distilled water, and a copper source and a complexing agent are added in sequence and stirred to obtain a dispersed solution.
4. The method for preparing a cuprous selenide nanozyme according to claim 3, characterized in that: The protective gas includes at least one of nitrogen or argon; The selenium source includes at least one of sodium selenite, selenium dioxide or selenium powder; The reducing agent includes at least one of sodium borohydride, ascorbic acid or hydrazine hydrate; The copper source includes at least one of copper chloride, copper sulfate or copper nitrate in different hydration states; The complexing agent includes at least one of polyvinyl pyrrolidone, ethylenediaminetetraacetic acid or sodium citrate.
5. The method for preparing a cuprous selenide nanozyme according to claim 3, characterized in that: The reaction temperature of the selenization solution is 25-60°C, the stirring speed is 200-500 rpm, and the stirring time is 30-120 minutes; The reaction temperature of the dispersed solution is 25-80° C., the stirring speed is 300-600 rpm, and the stirring time is 20-60 minutes.
6. The method for preparing a cuprous selenide nanozyme according to claim 1, characterized in that: The step of performing stability treatment on the dispersed solution to obtain a stable dispersed solution includes: adjusting the pH value of the dispersed solution by using a buffer solution, and heating the adjusted dispersed solution to obtain a stable dispersed solution.
7. The method for preparing a cuprous selenide nanozyme according to claim 6, characterized in that: The buffer solution includes at least one of a phosphate buffer, a borate buffer or a carbonate buffer; The heating method for heating the adjusted dispersed solution includes at least one of water bath heating, oil bath heating or heating magnetic stirrer, and the heating temperature is 50-80°C.
8. The method for preparing a cuprous selenide nanozyme according to claim 1, characterized in that: The frequency of ultrasonic treatment of the selenized solution is 20-40 kHz, and the time is 10-30 minutes.
9. The method for preparing a cuprous selenide nanozyme according to claim 1, characterized in that: The method of adding the dispersed selenized solution to the stable dispersed solution for mixing and separation, and taking the supernatant for purification and solidification to obtain the cuprous selenide nanozyme comprises: adding the dispersed selenide solution into the stable dispersed solution, and stirring and mixing the mixture to obtain a mixed solution; Centrifuging the mixed solution, allowing the mixed solution to stand after centrifugation, and extracting the supernatant of the mixed solution after standing; Purifying the supernatant to obtain purified cuprous selenide nanozyme, and solidifying the purified cuprous selenide nanozyme to obtain cuprous selenide nanozyme; Wherein, the purification comprises at least one of ultrafiltration or dialysis; and the solidification comprises at least one of freeze drying or vacuum drying.
10. An application of cuprous selenide nanozyme in photothermal applications, characterized in that: The cuprous selenide nanozyme is prepared by the preparation method of the cuprous selenide nanozyme described in any one of claims 1-9.