A reaction apparatus for simulating microbial consumption of hydrogen gas in a salt cavern and methods of use thereof

Abstract

The present application relates to the technical field of salt cave hydrogen storage, and in particular to a reaction device for simulating hydrogen consumption by microorganisms in a salt cave and a method for using the same. There is a lack of reaction equipment and methods for studying whether hydrogen is consumed by microorganisms in a salt cave in the prior art. The present application provides a reactor for simulating hydrogen consumption by microorganisms in a salt cave and a reaction device using the same. The special structure design of the reactor and the reaction device can achieve comparative testing of whether hydrogen is consumed by microorganisms and the amount of hydrogen consumption in the reaction device under salt-free and salt-containing reaction environments, thereby accurately determining the real impact of microorganisms on hydrogen storage in a salt cave.

Description

Technical Field

[0001] This invention relates to the field of hydrogen storage technology in salt caverns, specifically to a reaction device that simulates the consumption of hydrogen by microorganisms in salt caverns and its usage method. Background Technology

[0002] Hydrogen energy, as a low-carbon and zero-carbon energy source, is considered the most promising clean energy source of the 21st century. Storage and transportation of hydrogen are key technologies that urgently need to be addressed in the production, storage, and transportation stages. Among hydrogen storage technologies, underground hydrogen storage is widely recognized as the most likely technology to achieve large-scale storage.

[0003] The impact of microorganisms on underground hydrogen storage is significant. Studies have shown that hydrogen produced by both biological and abiotic processes can be consumed by microorganisms. In addition to the inherent underground microbial community, exogenous microorganisms may be introduced from surface gases or drilling fluids during hydrogen storage. Microorganisms are closely related to hydrogen consumption, production, and corrosion. Many microorganisms are considered major hydrogen consumers, such as methanogens, sulfate-reducing bacteria, and acetic acid bacteria. Hydrogen loss primarily occurs because microbial reactions convert H2 into gases such as CH4 or H2S.

[0004] Currently, there is limited research on whether underground microorganisms (especially those in salt caverns) consume hydrogen. There is also a lack of specialized equipment and methods to conduct qualitative and quantitative analyses of whether microorganisms in salt caverns consume hydrogen, making it impossible to determine the actual impact of microorganisms on hydrogen storage in salt caverns. Summary of the Invention

[0005] A problem with existing technologies is the lack of reaction equipment and methods for studying whether microorganisms in salt caverns consume hydrogen. To address this problem, this invention provides a reactor for simulating hydrogen consumption by microorganisms in salt caverns, comprising a reactor body, a reactor top cover, and sample dishes.

[0006] The reactor top cover is sealed and fixedly connected to the end face adjacent to the reactor body, forming a sealed accommodating cavity between the reactor top cover and the reactor body.

[0007] The sample dish is horizontally fixed at the upper part of the accommodating cavity;

[0008] The sample dish includes a fixing part, a supporting part, and a magnetic suction part, wherein the fixing part and the magnetic suction part are fixedly connected to the two ends of the supporting part, respectively.

[0009] The supporting part is rotatably and fixedly connected to the accommodating cavity through the fixing part;

[0010] The supporting part is magnetically attracted to the magnet on the outer wall of the reactor body through the magnetic attraction part, and forms a fixed connection with the accommodating cavity.

[0011] The reactor top cover surface is provided with two gas connection ports, which are interconnected with the accommodating cavity.

[0012] Preferably, a pressure gauge is also provided on the surface of the reaction top cover for detecting the gas pressure inside the accommodating cavity.

[0013] Preferably, a hanging ear is fixedly provided on the upper part of the accommodating cavity, and the fixing part of the sample dish has a hook corresponding to the hanging ear. The sample dish is hung on the hanging ear by the hook to form a rotatable and fixed connection with the accommodating cavity.

[0014] Preferably, the magnetic attraction part is fan-shaped, and the external magnet of the reactor body is arc-shaped.

[0015] Preferably, the orthographic projection of the sample dish support portion onto the horizontal plane is circular or elliptical.

[0016] Preferably, a slot is provided on the outer wall of the reactor body, and the magnet is located in the slot.

[0017] A reaction device for simulating the consumption of hydrogen by microorganisms in salt caverns, comprising the aforementioned reactor, circulating gas tank, gas circulation pipeline, and gas filling and sampling pipeline;

[0018] The two ends of the gas circulation pipeline are fixed at different positions on the top cover of the reactor. The gas circulation pipeline and the accommodating cavity of the reactor form a closed gas circulation channel. Gas enters the accommodating cavity of the reactor from one end of the gas circulation pipeline, and gas in the accommodating cavity enters the gas circulation pipeline from the other end of the gas circulation pipeline.

[0019] At least two circulation valves are installed on the circulation pipeline;

[0020] A circulating gas tank is provided between the two circulating valves. The circulating gas tank includes a tank body, with an upper interface and a lower interface at each end. The tank body is sealed and fixedly connected to the adjacent end face of the gas circulation pipeline through the upper and lower interfaces, respectively. The upper or lower interface is interconnected with the tank body. A heating and cooling jacket is provided on the outside of the tank body, forming a sealed interlayer cavity between the heating and cooling jacket and the tank body. The upper and lower parts of the interlayer cavity are respectively provided with an outlet and an inlet. The liquid medium enters the interlayer cavity from the inlet and then flows out from the outlet of the interlayer cavity.

[0021] One end of the gas-filled sampling pipeline is connected to the gas circulation pipeline, and the other end is connected to the gas collection device, gas generator, or vacuum device. A sampling valve is provided on the gas-filled sampling pipeline.

[0022] The connection between the gas sampling pipeline and the gas circulation pipeline is located near the upper interface of the circulating gas tank.

[0023] A reaction device for simulating the consumption of hydrogen by microorganisms in salt caverns, the method of use of which is as follows:

[0024] (1) Under nitrogen protection, place the sample salt at the bottom of the reactor cavity, then place the culture medium containing microorganisms above the surface of the sample dish support, place the magnet in the groove on the outer wall of the reactor body, and fix the sample dish by hanging it on the upper part of the reactor cavity with a hook. The other end of the sample dish with the magnetic suction part is placed in a position close to the magnet on the outside of the reactor body for fixing.

[0025] (2) Open all the circulation valves on the gas circulation pipeline and the sampling valve on the gas charging sampling pipeline. Connect the vacuum device to the gas charging sampling pipeline to perform vacuuming, so that the vacuum degree in the reactor cavity is not less than 0.1 MPa. Then connect the gas generator to the gas charging sampling pipeline and charge high-pressure hydrogen into the reactor cavity. When the pressure in the reactor cavity reaches 10-18 MPa, the charging is completed. Then repeat the above vacuuming and charging steps at least once. Then close the sampling valve and keep the circulation valve open.

[0026] (3) The reactor body is heated to the required reaction temperature by external heating. After the reaction has been carried out for a specified time, the reaction is stopped. A liquid medium is introduced into the heating and cooling jacket and kept warm for at least 5 minutes. There is a temperature difference between the liquid medium and the reactor. The existence of this temperature difference causes the gas in the gas circulation pipeline to spontaneously circulate in a ring.

[0027] (4) After that, close all the circulation valves, connect the gas collection device to the gas sampling pipeline, then open the sampling valve to collect and analyze the gas. After the gas collection is completed, evacuate the circulating gas tank and then close the sampling valve.

[0028] (5) After that, remove the magnet on the outer wall of the reactor, so that the sample dish is flipped downward around the hanging ear. The microbial sample on the surface of the sample dish falls into the bottom of the reactor cavity and reacts with the salt sample. After the reaction is completed for a specified time, all circulation valves are opened and liquid medium is introduced into the heating and cooling jacket for at least 5 minutes. The temperature of the liquid medium is different from that of the reactor. The existence of this temperature difference causes the gas in the gas circulation pipeline to spontaneously circulate in a ring.

[0029] (6) Finally, connect the sampler to the gas collection device, open the sampling valve, collect the gas in the reactor and perform gas analysis.

[0030] Preferably, the gas analysis method is gas chromatography.

[0031] Preferably, the reaction temperature in step (3) is 30-60℃.

[0032] Preferably, the temperature difference in step (5) is not less than 10°C.

[0033] The sample salt described in this invention can be a solid salt sample or a liquid salt sample of various concentrations. The microbial culture medium described in this invention can be a microbial liquid culture medium or an aqueous solution containing microorganisms.

[0034] The present invention has the following beneficial effects:

[0035] (1) The reactor designed by the present invention for simulating the consumption of hydrogen by microorganisms in a salt cave can simulate the high pressure, high humidity and high salt environment in an underground salt cave. Its special structural design allows microorganisms to react with hydrogen in a salt-free or salty environment in the reactor. By collecting the gas produced by the reaction of microorganisms with hydrogen in a salt-free or salty environment and performing gas chromatography analysis, the gas analysis results can be used to determine whether microorganisms react with hydrogen in a salt-free or salty environment and the amount of hydrogen consumed.

[0036] (2) The reaction device for simulating the consumption of hydrogen by microorganisms in salt caves designed by the present invention has an ingenious structure. By using two circulation valves on the gas circulation pipeline and the sampling valve on the gas filling sampling pipeline together, it can be realized that the pressure and gas composition in the reactor will not be affected during the first gas sampling analysis, and thus will not interfere with the second reaction in the reactor.

[0037] (3) The present invention has a circulating gas tank in the gas circulation pipeline of the reaction device for consuming hydrogen by microorganisms in a simulated salt cave. After each reaction, the circulating gas tank is heated or cooled to make the temperature difference between the circulating gas tank and the gas temperature in the reactor more than 10°C. This allows the gas generated in the reactor to spontaneously circulate and mix in the circulation channel formed by the reactor and the gas circulation pipeline. Then, sampling and analysis are performed to make the test results more accurate.

[0038] (4) The slope design of the sample dish support ensures that the microbial culture medium inside can be more easily transferred to the bottom of the container cavity of the reactor body, further ensuring the accuracy of the comparative test results. Attached Figure Description

[0039] Figure 1 This is a schematic diagram of the structure of a reactor that simulates the consumption of hydrogen by microorganisms in a salt cave, provided by the present invention.

[0040] Figure 2 :yes Figure 1A schematic diagram of the cross-sectional structure of the reactor body.

[0041] Figure 3 :yes Figure 1 A schematic diagram of the structure of the intermediate sample dish.

[0042] Figure 4 :yes Figure 3 A top view of the intermediate sample dish.

[0043] Figure 5 :yes Figure 3 A schematic diagram of the cross-sectional structure of the sample dish.

[0044] Figure 6 This is a schematic diagram of a reaction device for simulating the consumption of hydrogen by microorganisms in a salt cave, provided by the present invention.

[0045] Figure 7 :yes Figure 4 A schematic diagram of the structure of the medium-circulation gas tank.

[0046] In the diagram: 1. Pressure gauge, 2. Gas connection port, 3. Reactor top cover, 4. Reactor body, 5. Magnet, 6. Receiving cavity, 7. Sample dish, 7-1. Magnetic suction part, 7-2. Fixing part, 7-3-1. Horizontal support part, 7-3-2. Inclined support part, 7-4. Lifting rod, 8. Hanging lug, 9. Gas circulation pipeline, 10. Circulation valve, 11. Sampling valve, 12. Gas filling and sampling pipeline, 13. Circulating gas tank, 13-1. Heating and cooling jacket, 13-2. Gas tank body, 13-3. Upper interface, 13-4. Lower interface, 13-5. Water outlet, 13-6. Water inlet, 13-7. Jacket cavity, 14. Slot. Detailed Implementation

[0047] The present invention will be described in detail below with reference to embodiments. However, it should be understood that the following embodiments are merely illustrative examples of implementation of the present invention and are not intended to limit the scope of the present invention.

[0048] like Figure 1-7 The image shows a reactor and reaction apparatus for simulating the consumption of hydrogen by microorganisms in a salt cave, provided by the present invention. The reactor includes a reactor body, a reactor top cover, and a sample dish.

[0049] The reactor top cover is sealed and fixedly connected to the end face adjacent to the reactor body, forming a sealed accommodating cavity between the reactor top cover and the reactor body.

[0050] The sample dish is horizontally fixed at the upper part of the accommodating cavity;

[0051] The sample dish includes a fixing part, a supporting part, and a magnetic suction part, wherein the fixing part and the magnetic suction part are fixedly connected to the two ends of the supporting part, respectively.

[0052] The supporting part is rotatably and fixedly connected to the accommodating cavity through the fixing part;

[0053] The supporting part is magnetically attracted to the magnet on the outer wall of the reactor body through the magnetic attraction part, and forms a fixed connection with the accommodating cavity.

[0054] In one specific embodiment, the support portion includes a horizontal support portion and a tilted support portion. The angle formed between the lower surface of the tilted support portion and the plane is no greater than 45°, preferably 10-25°. The end faces of the horizontal support portion and the tilted support portion are fixedly connected. The other end of the horizontal support portion is fixedly connected to the fixing portion. The other end of the tilted support portion is fixedly connected to the magnetic suction portion. When the sample dish is horizontally fixed in the accommodating cavity of the reactor body, the plane where the horizontal support portion is located is parallel to the ground. When the magnet on the outer wall of the reactor body is removed, the sample dish flips downward around the fixing portion. The tilted support portion on the support portion makes it easier for the culture medium containing microorganisms on the upper surface of the support portion to be completely transferred to the bottom of the accommodating cavity of the reactor body.

[0055] In one specific embodiment, the upper surface of the support is also provided with a lifting rod, which makes it easier to pick up or place the sample dish.

[0056] The reactor top cover surface is provided with two gas connection ports, which are interconnected with the accommodating cavity.

[0057] In one specific embodiment, a pressure gauge is also provided on the surface of the reaction top cover for detecting the gas pressure inside the accommodating cavity.

[0058] In one specific embodiment, a hanging ear is fixedly provided on the upper part of the accommodating cavity, and the fixing part of the sample dish has a hook corresponding to the hanging ear. The sample dish is hung on the hanging ear by the hook to form a rotatable and fixed connection with the accommodating cavity.

[0059] In one specific embodiment, the magnetic attraction part is fan-shaped, and the external magnet of the reactor body is arc-shaped.

[0060] In one specific embodiment, the orthographic projection shape of the sample dish support portion on the horizontal plane is circular or elliptical.

[0061] In one specific embodiment, a slot is provided on the outer wall of the reactor body, and the magnet is located in the slot.

[0062] A reaction device for simulating the consumption of hydrogen by microorganisms in salt caverns, comprising the aforementioned reactor, circulating gas tank, gas circulation pipeline, and gas filling and sampling pipeline;

[0063] The two ends of the gas circulation pipeline are fixed at different positions on the top cover of the reactor. The gas circulation pipeline and the accommodating cavity of the reactor form a closed gas circulation channel. Gas enters the accommodating cavity of the reactor from one end of the gas circulation pipeline, and gas in the accommodating cavity enters the gas circulation pipeline from the other end of the gas circulation pipeline.

[0064] At least two circulation valves are installed on the circulation pipeline;

[0065] A circulating gas tank is provided between the two circulating valves. The circulating gas tank includes a tank body, with an upper interface and a lower interface at each end. The tank body is sealed and fixedly connected to the adjacent end face of the gas circulation pipeline through the upper and lower interfaces, respectively. The upper or lower interface is interconnected with the tank body. A heating and cooling jacket is provided on the outside of the tank body, forming a sealed interlayer cavity between the heating and cooling jacket and the tank body. The upper and lower parts of the interlayer cavity are respectively provided with an outlet and an inlet. The liquid medium enters the interlayer cavity from the inlet and then flows out from the outlet of the interlayer cavity.

[0066] One end of the gas-filled sampling pipeline is connected to the gas circulation pipeline, and the other end is connected to the gas collection device, gas generator, or vacuum device. A sampling valve is provided on the gas-filled sampling pipeline.

[0067] The connection between the gas sampling pipeline and the gas circulation pipeline is located near the upper interface of the circulating gas tank.

[0068] The method of using the simulated salt cavern microbial hydrogen consumption reaction device is as follows:

[0069] (1) Under nitrogen protection, place the sample salt at the bottom of the reactor cavity, then place the culture medium containing microorganisms above the surface of the sample dish support, place the magnet in the groove on the outer wall of the reactor body, and fix the sample dish by hanging it on the upper part of the reactor cavity with a hook. The other end of the sample dish with the magnetic suction part is placed in a position close to the magnet on the outside of the reactor body for fixing.

[0070] (2) Open all the circulation valves on the gas circulation pipeline and the sampling valve on the gas charging sampling pipeline. Connect the vacuum device to the gas charging sampling pipeline to perform vacuuming, so that the vacuum degree in the reactor cavity is not less than 0.1 MPa. Then connect the gas generator to the gas charging sampling pipeline and charge high-pressure hydrogen into the reactor cavity. When the pressure in the reactor cavity reaches 10-18 MPa, the charging is completed. Then repeat the above vacuuming and charging steps at least once. Then close the sampling valve and keep the circulation valve open.

[0071] (3) The reactor body is heated to the required reaction temperature by external heating. After the reaction has been carried out for a specified time, the reaction is stopped. A liquid medium is introduced into the heating and cooling jacket and kept warm for at least 5 minutes. There is a temperature difference between the liquid medium and the reactor. The existence of this temperature difference causes the gas in the gas circulation pipeline to spontaneously circulate in a ring.

[0072] (4) After that, close all the circulation valves, connect the gas collection device to the gas sampling pipeline, then open the sampling valve to collect and analyze the gas. After the gas collection is completed, evacuate the circulating gas tank and then close the sampling valve.

[0073] (5) After that, remove the magnet on the outer wall of the reactor, so that the sample dish is flipped downward around the hanging ear. The microbial sample on the surface of the sample dish falls into the bottom of the reactor cavity and reacts with the salt sample. After the reaction is completed for a specified time, all circulation valves are opened and liquid medium is introduced into the heating and cooling jacket for at least 5 minutes. The temperature of the liquid medium is different from that of the reactor. The existence of this temperature difference causes the gas in the gas circulation pipeline to spontaneously circulate in a ring.

[0074] (6) Finally, connect the sampler to the gas collection device, open the sampling valve, collect the gas in the reactor and perform gas analysis.

[0075] In one specific embodiment, the gas analysis method is gas chromatography.

[0076] In one specific embodiment, the reaction temperature in step (3) is 30-60°C.

[0077] In one specific embodiment, the temperature difference in step (5) is not less than 10°C.

[0078] Based on the above-described preferred embodiments of the present invention, and through the foregoing description, those skilled in the art can make various changes and modifications without departing from the inventive concept. The technical scope of this invention is not limited to the contents of the specification, but must be determined according to the scope of the claims.

Claims

1. A reaction apparatus for simulating the consumption of hydrogen by microorganisms in salt caverns, characterized in that, This includes a circulating gas tank, gas circulation pipeline, gas filling and sampling pipeline, and reactor; The two ends of the gas circulation pipeline are fixed at different positions on the top cover of the reactor. A closed gas circulation channel is formed between the gas circulation pipeline and the containment cavity of the reactor. Gas enters the containment cavity of the reactor from one end of the gas circulation pipeline, and gas in the containment cavity enters the gas circulation pipeline from the other end of the gas circulation pipeline. At least two circulation valves should be installed on the circulation pipeline; A circulating gas tank is installed between the two circulating valves. The circulating gas tank includes a tank body, with an upper interface and a lower interface at each end. The tank body is sealed and fixedly connected to the adjacent end face of the gas circulation pipeline through the upper and lower interfaces, respectively. Both the upper and lower interfaces are interconnected with the tank body. A heating and cooling jacket is installed on the outside of the tank body, forming a sealed interlayer cavity between the heating and cooling jacket and the tank body. The upper and lower parts of the interlayer cavity are respectively provided with an outlet and an inlet. The liquid medium enters the interlayer cavity from the inlet and then flows out from the outlet of the interlayer cavity. One end of the gas-filled sampling pipeline is connected to the gas circulation pipeline, and the other end is connected to the gas collection device, gas generator, or vacuum device. A sampling valve is installed on the gas-filled sampling pipeline. The connection point between the inflation sampling pipeline and the gas circulation pipeline is located near the upper interface of the circulating gas tank. The reactor includes a reactor body, a reactor top cover, and a sample dish. The reactor top cover is sealed and fixedly connected to the adjacent end face of the reactor body, forming a sealed cavity between the reactor top cover and the reactor body. The sample dish is horizontally fixed at the top of the cavity. The sample dish includes a fixing part, a supporting part, and a magnetic suction part. The fixing part and the magnetic suction part are fixedly connected to the two ends of the supporting part, respectively. The supporting part is rotatably fixedly connected to the cavity through the fixing part. The supporting part is magnetically attracted to the cavity through the magnetic suction part and a magnet provided on the outer wall of the reactor body. The reactor top cover has two gas connection ports that communicate with the cavity.

2. The reaction apparatus for simulating the consumption of hydrogen by microorganisms in a salt cave as described in claim 1, characterized in that, The magnetic attraction part is fan-shaped, and the magnet on the outside of the reactor body is arc-shaped.

3. The reaction apparatus for simulating the consumption of hydrogen by microorganisms in a salt cave as described in claim 1, characterized in that, The orthographic projection of the sample dish support on the horizontal plane is circular or elliptical.

4. The reaction apparatus for simulating the consumption of hydrogen by microorganisms in a salt cave as described in claim 1, characterized in that, A pressure gauge is also installed on the surface of the reactor top cover to detect the gas pressure inside the cavity.

5. The reaction apparatus for simulating the consumption of hydrogen by microorganisms in a salt cave as described in claim 4, characterized in that, The upper part of the accommodating cavity is fixedly provided with a hanging ear, and the fixing part of the sample dish has a hook corresponding to the hanging ear. The sample dish is hung on the hanging ear by the hook and forms a rotating and fixed connection with the accommodating cavity.

6. The reaction apparatus for simulating the consumption of hydrogen by microorganisms in a salt cave as described in claim 5, characterized in that, The outer wall of the reactor body is provided with a slot, and the magnet is located in the slot.

7. A method for simulating the consumption of hydrogen by microorganisms in salt caverns, characterized in that, The reaction apparatus for simulating the consumption of hydrogen by microorganisms in a salt cave as described in claim 6 is used in the following method: (1) Under nitrogen protection, place the sample salt at the bottom of the reactor cavity, then place the culture medium containing microorganisms above the surface of the sample dish support, place the magnet in the groove on the outer wall of the reactor body, and fix the sample dish by hanging it on the upper part of the reactor cavity with a hook. The other end of the sample dish with the magnetic suction part is placed in a position close to the magnet on the outside of the reactor body for fixing. (2) Open all circulation valves on the gas circulation pipeline and sampling valves on the gas charging sampling pipeline. Connect the vacuum device to the gas charging sampling pipeline to perform vacuuming, so that the vacuum degree in the reactor cavity is not less than 0.1 MPa. Then connect the gas generator to the gas charging sampling pipeline and charge high-pressure hydrogen into the reactor cavity. When the pressure in the reactor cavity reaches 10-18 MPa, the charging is completed. Then repeat the above vacuuming and charging steps at least once. Then close the sampling valve and keep the circulation valve open. (3) The reactor body is heated to the required reaction temperature by external heating. After the reaction has been carried out for a specified time, the reaction is stopped. Liquid medium is introduced into the heating and cooling jacket and kept warm for at least 5 minutes. There is a temperature difference between the liquid medium and the reactor. The existence of this temperature difference causes the gas in the gas circulation pipeline to spontaneously circulate in a ring. (4) After that, close all the circulation valves, connect the gas collection device to the gas charging sampling pipeline, then open the sampling valve to collect and analyze the gas. After the gas collection is completed, evacuate the circulating gas tank and then close the sampling valve. (5) After that, remove the magnet on the outer wall of the reactor so that the sample dish is flipped downward around the hanging ear. The microbial sample on the surface of the sample dish falls into the bottom of the reactor cavity and reacts with the salt sample. After the reaction is completed for the specified time, all circulation valves are opened and liquid medium is introduced into the heating and cooling jacket for at least 5 minutes. There is a temperature difference between the liquid medium and the reactor. The existence of this temperature difference causes the gas in the gas circulation pipeline to spontaneously circulate in a ring. (6) Finally, connect the sampler to the gas collection device, open the sampling valve, collect the gas in the reactor and perform gas analysis.

8. A method for simulating the consumption of hydrogen by microorganisms in a salt cave according to claim 7, characterized in that, The reaction temperature in step (3) is 30-60℃.

9. A method for simulating the consumption of hydrogen by microorganisms in a salt cave according to claim 8, characterized in that, The temperature difference in step (5) shall not be less than 10℃.

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