Position calibration system

Abstract

The invention discloses a position calibration system, which is used for nucleic acid detection equipment with a motion module, and comprises: a calibration tool adapted to the nucleic acid detection equipment and used for detecting the motion module; the nucleic acid detection equipment and the calibration tool are in communication connection with the calibration operation device; the calibration operation device is configured to send a position calibration instruction to the nucleic acid detection equipment, the position calibration instruction at least comprises a to-be-calibrated bit, and the to-be-calibrated bit at least comprises an amplification bit and an extraction bit; executing position calibration operation of the amplification bit according to the position calibration instruction, and obtaining an amplification calibration value of the amplification bit; executing a position calibration operation of the extraction bit according to the position calibration instruction, and obtaining an extraction calibration value of the extraction bit; and outputting the amplification calibration value and the extraction calibration value to the nucleic acid detection equipment. Through cooperation of the nucleic acid detection equipment, the calibration tool and the calibration operation device, full-automatic calibration of an amplification site and an extraction site can be realized, the calibration technology level of a user is not depended on, and the calibration precision is relatively high.

Description

Technical Field

[0001] The present invention belongs to the technical field of position calibration, and particularly relates to a position calibration system. Background Art

[0002] With the progress of technology, sample detection devices such as POCT integrated machines are more and more widely used. Point-of-care testing (POCT) is a testing method that is carried out at the sampling site and uses portable analytical instruments and supporting reagents to quickly obtain test results. In the existing scenario, the position calibration before the sample detection device leaves the factory is carried out by manual assistance with manual tooling. Since the calibration process is entirely controlled by humans, the accuracy of calibration highly depends on the professional skills of the user, not only taking a long time and having poor consistency, but also having low calibration accuracy. Moreover, after the sample detection device has been used at the client for a period of time, due to the high professional technical requirements, the customer cannot calibrate the instrument by themselves, which greatly affects the after-sales maintenance cost and the customer experience. Summary of the Invention

[0003] The purpose of the present invention is to provide a position calibration system to solve the technical problem of low calibration accuracy of nucleic acid detection devices in the prior art.

[0004] To achieve the above purpose, on the one hand, the present invention provides a position calibration system for a nucleic acid detection device with a motion module, and the position calibration system includes:

[0005] A calibration tooling, adapted to the nucleic acid detection device and used to detect the motion module;

[0006] A calibration operation device, the nucleic acid detection device, the calibration tooling are all communicatively connected to the calibration operation device;

[0007] The calibration operation device is configured to:

[0008] Send a position calibration instruction to the nucleic acid detection device, the position calibration instruction at least includes the position to be calibrated, and the position to be calibrated at least includes an amplification position and an extraction position;

[0009] Execute the position calibration operation of the amplification position according to the position calibration instruction, and obtain the amplification calibration value of the amplification position;

[0010] Execute the position calibration operation of the extraction position according to the position calibration instruction, and obtain the extraction calibration value of the extraction position;

[0011] Output the amplification calibration value and the extraction calibration value to the nucleic acid detection device.

[0012] In an embodiment of the present invention, the amplification position at least includes a Y-axis amplification position, the extraction position at least includes a Y-axis extraction position and a Z-axis extraction position, and the calibration operation device is further configured to:

[0013] Perform a position calibration operation on the Y-axis amplification position according to the position calibration instruction;

[0014] Perform a position calibration operation on the Y-axis extraction position according to the position calibration instruction;

[0015] Determine that the calibration of the Y-axis extraction position is successful, and perform a position calibration operation on the Z-axis extraction position according to the position calibration instruction.

[0016] In an embodiment of the present invention, the calibration operation device is further configured to:

[0017] Obtain the default Y-axis extraction position of the Y-axis extraction position;

[0018] Control the motion module to move to the default Y-axis extraction position;

[0019] Obtain the default Z-axis position of the Z-axis extraction position;

[0020] Control the motion module to move to the default Z-axis position;

[0021] Control the motion module to move along the Z-axis and obtain the current position of the Z-axis extraction position.

[0022] In an embodiment of the present invention, the position calibration instruction further includes the number of calibrations, and the calibration operation device is further configured to:

[0023] Loop the position calibration operations of the amplification position and the extraction position according to the number of calibrations;

[0024] Obtain the single calibration value of the amplification position and the extraction position;

[0025] Calculate the calibration mean value of the single calibration value.

[0026] In an embodiment of the present invention, the calibration operation device is further configured to:

[0027] Obtain the default value of the position to be calibrated;

[0028] Determine the maximum offset value and the minimum offset value according to the default value and the single calibration value;

[0029] Calculate the calibration difference between the maximum offset value and the minimum offset value;

[0030] Determine that the calibration difference is less than the preset difference, calculate the calibration mean value of the single calibration value and use it as the calibration value.

[0031] In an embodiment of the present invention, an initial position is set on the nucleic acid detection device, and the calibration operation device is configured to:

[0032] Determine that the position calibration of the amplification position is successful, and control the motion module to reset to the initial position;

[0033] Perform the position calibration operation of the extraction position.

[0034] In an embodiment of the present invention, the calibration operation device is further configured to:

[0035] Obtain the amplification default value and the amplification calibration value of the amplification position;

[0036] Calculate the amplification offset value of the amplification position;

[0037] Obtain the extraction default value of the extraction position;

[0038] According to the extraction default value and the amplification offset value, control the motion module to drive the calibration tooling.

[0039] In an embodiment of the present invention, the calibration tooling includes a profiling main body and a first detector installed on the profiling main body, and the first detector is used to detect the current positions of the Y-axis extraction position and the Z-axis extraction position.

[0040] In an embodiment of the present invention, the calibration tooling further includes a position adjustment and measurement member and a second detector installed on the profiling main body. Under the pushing of the nucleic acid detection device, the position adjustment and measurement member cooperates with the second detector to obtain the current position of the Y-axis amplification position.

[0041] In an embodiment of the present invention, both the first detector and the second detector are photoelectric switches.

[0042] Through the above technical solutions, the position calibration system provided by the embodiments of the present invention has the following beneficial effects:

[0043] When using the position calibration system in this embodiment for position calibration, the nucleic acid detection device, the calibration tooling and the calibration operation device are all communicatively connected. The calibration operation device can determine that the calibration tooling enters the nucleic acid detection device according to the tooling detection information of the nucleic acid detection device, and send a position calibration instruction with the position to be calibrated to the nucleic acid detection device. It can control the motion module to drive the calibration tooling to first perform the position calibration operation of the amplification position according to the position calibration instruction, and obtain the amplification calibration value of the amplification position; then perform the position calibration operation of the extraction position according to the position calibration instruction, and obtain the extraction calibration value of the extraction position. Finally, it outputs the amplification calibration value and the extraction calibration value to the nucleic acid detection device, which can realize the full-automatic calibration of the amplification position and the extraction position, does not depend on the user's calibration technical level, and has a relatively high calibration accuracy.

[0044] Other features and advantages of the embodiments of the present invention will be described in detail in the subsequent specific embodiment part. Description of the Drawings

[0045] The drawings are used to provide a further understanding of the embodiments of the present invention, and constitute a part of the specification. Together with the following specific embodiments, they are used to explain the embodiments of the present invention, but do not constitute a limitation to the embodiments of the present invention. For those of ordinary skill in the art, other drawings can be obtained based on the structures shown in these drawings without creative efforts. In the drawings:

[0046] Figure 1 is a calibration operation flowchart of a position calibration system according to an embodiment of the present invention;

[0047] Figure 2 is a schematic diagram of a calibration operation process of a position calibration system according to another embodiment of the present invention;

[0048] Figure 3 is a calibration operation flowchart of a position calibration system according to still another embodiment of the present invention;

[0049] Figure 4 is a schematic diagram of the structure of a position calibration system according to an embodiment of the present invention;

[0050] Figure 5 is a schematic diagram of the structure of a position calibration system according to another embodiment of the present invention;

[0051] Figure 6 is a schematic diagram of the structure of a calibration tooling of a position calibration system according to an embodiment of the present invention;

[0052] Figure 7 is a schematic diagram of the structure of a calibration tooling of a position calibration system according to an embodiment of the present invention;

[0053] Figure 8 is a schematic diagram of the structure of a calibration tooling of a position calibration system according to another embodiment of the present invention;

[0054] Figure 9 is a schematic diagram of the structure of a calibration tooling of a position calibration system according to another embodiment of the present invention;

[0055] Figure 10 is a schematic diagram of the structure of a position calibration system according to an embodiment of the present invention.

[0056] Description of Reference Numerals

[0057] Label Name Label Name

[0058] 100 Calibration Tooling 4 Second Detector

[0059] 1 Profiling Body 5 Third Detector

[0060] 11 Sliding Cavity 6 Elastic Resetting Member

[0061] 12 Upper Mounting Cavity 7 Temperature Probe

[0062] 2 Position Adjustment Component 8 Temperature Detector

[0063] 21 Sliding Body 200 Nucleic Acid Detection Device

[0064] 22 Limit Ear 210 Pipette Tip

[0065] 3 First Detector Detailed Implementation Manner

[0066] The following details the specific implementation manner of the present invention in conjunction with the accompanying drawings. It should be understood that the specific implementation manner described herein is only for explaining and illustrating the present invention and is not used to limit the present invention.

[0067] Next, refer to the attached Figures 1 to 10 Describe a position calibration system according to the present invention for a nucleic acid detection device 200 with a motion module. The position calibration system includes:

[0068] A calibration tooling 100, adapted to the nucleic acid detection device 200 and used for detecting the motion module;

[0069] A calibration operation device, to which the nucleic acid detection device 200 and the calibration tooling 100 are both communicatively connected;

[0070] As Figure 1 shown, in one embodiment, the calibration operation device is configured to:

[0071] Send a position calibration instruction to the nucleic acid detection device 200, where the position calibration instruction at least includes the positions to be calibrated, and the positions to be calibrated at least include the amplification position and the extraction position;

[0072] Execute the position calibration operation for the amplification position according to the position calibration instruction and obtain the amplification calibration value of the amplification position;

[0073] Execute the position calibration operation for the extraction position according to the position calibration instruction and obtain the extraction calibration value of the extraction position;

[0074] Output the amplification calibration value and the extraction calibration value to the nucleic acid detection device 200.

[0075] Understandably, the Y-axis is the front-back direction, the Z-axis is the up-down direction, and the nucleic acid detection device 200 can be a POCT integrated machine with a pipetting module. The nucleic acid detection device 200 further includes an experiment module for sample detection. In this embodiment, the experiment module is a PCR module. The calibration operation device is further configured to: determine that the calibration tooling 100 enters the nucleic acid detection device 200 and lock the experiment module. The nucleic acid detection device 200 in this embodiment can detect whether the calibration tooling 100 is in place and send a tooling in-place message to the calibration operation device. When the calibration operation device determines that the calibration tooling 100 is in place, it can lock the experiment module and prohibit the experiment operation of the experiment module, avoiding the situation of incorrect operation of the nucleic acid detection device 200 by the user during the calibration process, and improving the calibration safety of the calibration system. The specific positions of the amplification position and the extraction position in this embodiment can be specifically set on the nucleic acid detection device 200 according to actual usage requirements.

[0076] The motion module can drive the calibration tooling 100 to move along the Y-axis (front-back direction). The nucleic acid detection device 200 further includes a device control module, a communication module, and a pipetting module with a pipette pump. The motion module can drive the pipette tip 210 of the pipette pump to move along the Z-axis (up-down direction). The calibration operation device can send a position calibration instruction to the device control module. The device control module can generate a motion module control instruction during the position calibration according to the position calibration instruction.

[0077] When using the position calibration system in this embodiment for position calibration, the nucleic acid detection device 200, the calibration tooling 100, and the calibration operation device are all communicatively connected. The calibration operation device can determine that the calibration tooling 100 enters the nucleic acid detection device 200 according to the tooling detection information of the nucleic acid detection device 200, and send a position calibration instruction with the position to be calibrated to the nucleic acid detection device 200. It can control the motion module to drive the calibration tooling 100 to first perform the position calibration operation of the amplification position according to the position calibration instruction and obtain the amplification calibration value of the amplification position; then perform the position calibration operation of the extraction position according to the position calibration instruction and obtain the extraction calibration value of the extraction position. Finally, it outputs the amplification calibration value and the extraction calibration value to the nucleic acid detection device 200, which can realize the full-automatic calibration of the amplification position and the extraction position, is independent of the user's calibration technical level, and has a relatively high calibration accuracy.

[0078] The calibration tooling 100 in this embodiment imitates the consumables of the nucleic acid detection device 200, and the amplification position corresponds to the position where the consumables are docked with the PCR module. A pipetting module is also provided on the nucleic acid detection device 200, and the extraction position corresponds to the position where the pipetting module is docked with the consumables.

[0079] Specifically, the amplification position at least includes a Y-axis amplification position, and the extraction position at least includes a Y-axis extraction position and a Z-axis extraction position. As Figure 2 shown, the calibration operation device is further configured to:

[0080] Perform the position calibration operation of the Y-axis amplification position according to the position calibration instruction;

[0081] Perform the position calibration operation of the Y-axis extraction position according to the position calibration instruction;

[0082] Determine that the calibration of the Y-axis extraction position is successful, and perform the position calibration operation of the Z-axis extraction position according to the position calibration instruction.

[0083] In this embodiment, specifically, first calibrate the Y-axis amplification position. When it is determined that the Y-axis amplification position is successful, then calibrate the Y-axis extraction position. When it is determined that the calibration of the Y-axis extraction position is successful, perform the position calibration operation of the Z-axis extraction position according to the position calibration instruction. In this embodiment, calibrate the Y-axis amplification position, the Y-axis extraction position, and the Z-axis extraction position in sequence, optimize the calibration sequence, ensure that the calibration tooling 200 can detect the motion module, and improve the calibration accuracy and efficiency.

[0084] In this embodiment, first determine that the calibration of the amplification position is successful before performing the position calibration operation of the extraction position. In the case of failure of the position calibration of the amplification position, a calibration failure alarm can be given and the motion module can be shut down, which can avoid the situation where the extraction position calibration operation is still performed when the amplification position fails, and can improve the calibration efficiency and accuracy.

[0085] Furthermore, the calibration operation device is further configured to:

[0086] Obtain the default Y-axis extraction position of the Y-axis extraction position;

[0087] Control the motion module to move to the default Y-axis extraction position;

[0088] Obtain the default Z-axis position of the Z-axis extraction position;

[0089] Control the motion module to move to the default Z-axis position;

[0090] Control the motion module to move along the Z-axis and obtain the current position of the Z-axis extraction position.

[0091] Specifically, when performing the position calibration operation of the Z-axis extraction position, first control the motion module to drive the calibration tooling 100 to move to the default Y-axis extraction position, and then control the motion module to drive the pipette tip 210 to move to the default Z-axis position to ensure that the pipette tip 210 extends into the calibration tooling 100, and the calibration tooling 100 can detect the pipette tip 210, and then drive the pipette tip 210 to move upward in a step-by-step manner until the calibration tooling 100 can no longer detect the pipette tip 210.

[0092] It should be noted that the position calibration instruction also includes the number of calibrations. As Figure 3 shown, the calibration operation device is further configured to:

[0093] Perform position calibration operations for the amplification position and extraction position in a loop according to the number of calibrations;

[0094] Obtain the single calibration value of the amplification position and extraction position;

[0095] Calculate the calibration mean value of the single calibration value.

[0096] The number of calibrations in this embodiment can be preset, and the user can also input the number of calibrations to the calibration operation device. When performing position calibration, the nucleic acid detection device 200 and the calibration tooling 100 are both communicatively connected to the calibration operation device. The calibration operation device can determine that the calibration tooling 100 enters the nucleic acid detection device 200 according to the tooling detection information of the nucleic acid detection device 200, and send a position calibration instruction with the position to be calibrated and the number of calibrations to the nucleic acid detection device 200. It can control the motion module to drive the calibration tooling 100 to perform position calibration operations on the position to be calibrated and obtain the single calibration value. After performing the cyclic position calibration operation according to the number of calibrations, the calibration mean value of multiple calibration operations is calculated as the final calibration value. In this embodiment, through the cooperation of the nucleic acid detection device 200, the calibration tooling 100 and the calibration operation device, multiple position calibration operations can be performed, and the single calibration value can be averaged, which can not only achieve automatic calibration, but also improve the calibration accuracy. In this embodiment, the position calibration operations of the amplification position and the extraction position are used as a single position calibration operation unit, which facilitates the cycle of single position calibration operations.

[0097] In an embodiment of the present invention, the calibration operation device is further configured to:

[0098] Obtain the default value of the position to be calibrated;

[0099] Determine the maximum offset value and the minimum offset value according to the default value and the single calibration value;

[0100] Calculate the calibration difference between the maximum offset value and the minimum offset value;

[0101] Determine that the calibration difference is less than the preset difference, calculate the calibration mean value of the single calibration value and use it as the calibration value.

[0102] After completing the cycle of single position calibration operations in this embodiment, obtain the maximum offset value and the minimum offset value among the default value and multiple single calibration values, and compare and calculate the calibration difference between the maximum offset value and the minimum offset value, so as to determine the calibration difference between single position calibration operations. When it is determined that the calibration difference is less than the preset difference, calculate the calibration mean value of the single calibration value and use it as the calibration value. In the case where it is determined that the calibration difference is greater than or equal to the preset difference, a calibration failure alarm can be issued. In this embodiment, by comparing the calibration offset values in multiple calibration operations, the situation where a large single calibration offset value affects the calibration mean value can be avoided, and the calibration accuracy is further improved.

[0103] In one embodiment, an initial position is set on the nucleic acid detection device 200, and the calibration operation device is configured to:

[0104] Determine that the position calibration of the amplification position is successful, and control the motion module to reset to the initial position;

[0105] Perform the position calibration operation of the extraction position.

[0106] In this embodiment, the initial position is a preset position, which can be set in advance according to actual usage requirements. When the position calibration of the amplification position is successful, the calibration operation device can control the motion module to reset to the initial position, zero for the subsequent position calibration operation. A zeroing optoelectronic switch can be set on the nucleic acid detection device 200 to detect the calibration tooling 100 located at the initial position. The calibration tooling 100 is reset driven by the motion module, and the calibration operation device can obtain the zeroing information of the zeroing optoelectronic switch. When it is determined that the motion module drives the calibration tooling 100 to reset to the initial position, the position operation of the extraction position is started.

[0107] In another embodiment, the calibration operation device is further configured to:

[0108] Obtain the amplification default value and the amplification calibration value of the amplification position;

[0109] Calculate the amplification offset value of the amplification position;

[0110] Obtain the extraction default value of the extraction position;

[0111] Control the motion module to drive the calibration tooling 100 according to the extraction default value and the amplification offset value.

[0112] The amplification calibration value in this embodiment is the current position of the current position calibration operation, which can be detected by the calibration tooling 100. In this embodiment, the initial position is cancelled. When it is determined that the position calibration of the amplification position is successful, the amplification default value and the amplification calibration value of the amplification position are obtained, and the amplification offset value is calculated. The motion module is controlled to drive the calibration tooling 100 to move according to the amplification offset value and the extraction default value of the extraction position, which can avoid the situation of resetting after the amplification position calibration operation is completed. And combined with the amplification offset value of the amplification position, it can avoid the deviation interference of the amplification position, and at the same time omit the reset process, which can improve the calibration efficiency and shorten the calibration time.

[0113] In an embodiment of the present invention, the calibration tooling 100 includes a profiling main body 1 and a first detector 3 installed on the profiling main body 1. The first detector 3 is used to detect the current positions of the Y-axis extraction position and the Z-axis extraction position.

[0114] The first detector 3 in this embodiment can be an optoelectronic switch. When calibrating the extraction position, it can first perform Y-axis extraction position calibration. By detecting the pipette tip 210 of the pipetting module with the first detector 3, the calibration operation device can obtain the optoelectronic signal of the first detector 3, and obtain the current position information of the pipette tip 210 through the optoelectronic signal. The first detector 3 can detect the Y-axis extraction position and the Z-axis extraction position by detecting the pipette tip 210 and generate optoelectronic information. The in-place detection of the Y-axis extraction position and the Z-axis extraction position share the same optoelectronic switch, which has a simple structure. And when it is determined that the first detector 3 successfully calibrates the Y-axis extraction position, the Z-axis extraction position calibration is carried out, which can avoid the situation that the Z-axis extraction cannot be detected due to the deviation of the Y-axis extraction position, and improves the detection accuracy.

[0115] In the embodiment of the present invention, the calibration tooling 100 further includes a position adjustment member 2 installed on the profiling main body 1 and a second detector 4. Under the pushing of the nucleic acid detection device 200, the position adjustment member 2 cooperates with the second detector 4 to obtain the current position of the Y-axis amplification position.

[0116] When the calibration operation device in this embodiment controls the motion module to move towards the amplification position, the PCR module of the nucleic acid detection device 200 can push the position adjustment member 2, causing the position adjustment member 2 to displace along the Y-axis and block the second detector 4. The position adjustment member 2 and the second detector 4 cooperate to detect the current position of the amplification position. The calibration operation device can obtain the current position of the Y-axis amplification position through the change of the optoelectronic signal of the second detector 4.

[0117] Figures 6 to 9 As shown, the calibration tooling 100 further includes an elastic reset member 6. The elastic reset member 6 is located between the position adjustment member 2 and the profiling main body 1. The position adjustment member 2 can cooperate with the profiling main body 1 to compress the elastic reset member 6 under the pushing of the PCR module, and the elastic reset member 6 can drive the position adjustment member 2 to reset.

[0118] The profiling main body 1 is provided with a sliding cavity 11. The position adjustment member 2 is accommodated in the sliding cavity 11. One end of the sliding cavity 11 is provided with a telescopic opening, and the other end of the sliding cavity 11 is provided with a limit stop block for limiting the position adjustment member 2. The position adjustment member 2 includes a sliding main body 21 and a limit ear 22. The sliding main body 21 extends along the Y-axis; the limit ear 22 protrudes from the edge of the sliding main body 21 along the Z-axis, and the sliding cavity 11 is provided with a limit portion corresponding to the limit ear 22, and the size of the limit portion is larger than the size of the limit ear 22.

[0119] As Figures 8 to 9As shown, in one embodiment, the calibration tooling 100 further includes a third detector 5. The third detector 5 can be an optoelectronic sensor, which is used to detect the current position of the extraction position in the S direction. The position with a detected state of 0 by the optoelectronic sensor means not blocked, and 1 means blocked by the pump head. The first detector 3, the second detector 4, and the third detector 5 are all optoelectronic sensors; and an upper mounting cavity 12 is provided at the upper end of the profiling body 1, and the first detector 3 and the third detector 5 are both accommodated in the upper mounting cavity 12.

[0120] A temperature probe 7 for adapting to the PCR module is provided at the front end of the calibration tooling 100 in the Y-axis direction. The temperature probe 7 is detachably connected to the profiling body 1 and can detect the heating temperature of the PCR module, so as to facilitate the temperature calibration of the calibration operation device. In one embodiment, a heating module is provided on the nucleic acid detection device 200, and a temperature detector 8 for detecting the heating temperature of the heating module can be installed on the profiling body 1, which can facilitate the heating calibration of the calibration operation device for the heating module.

[0121] During the calibration process of the Y-axis amplification position, the calibration operation device first sends the default Y-axis amplification position to the motion module. The default Y-axis amplification position is equal to the default Y-axis amplification value + the Y-axis calibration range value, so that the calibration tooling 100 moves to the default Y-axis amplification position, and it is judged whether the second detector 4 is set (that is, an optoelectronic in-place signal is generated by being blocked). In the case where the second detector 4 is set in the first judgment, a Y-axis calibration out-of-range warning is given.

[0122] Specifically, the calibration operation device determines that the second detector 4 is not set, and controls the motion module to perform a step-by-step movement from the default Y-axis amplification position to the preset Y-axis position; determines that the current position exceeds the preset Y-axis position, shuts down the motion module and gives a calibration failure warning.

[0123] During the calibration process of the Y-axis amplification position, the calibration operation device controls the motion module to drive the calibration tooling 100 to move to the default Y-axis amplification position, and judges whether the second detector 4 is set. In the case where the second detector 4 is not set, the calibration operation device controls the motion module to perform a step-by-step movement from the default Y-axis amplification position to the preset Y-axis position, adjusts with the minimum adjustment distance (which can be preset in advance), and judges whether the current position exceeds the preset Y-axis position. The preset Y-axis position is equal to the default Y-axis amplification value - the amplification calibration range value. In the case where the current position exceeds the preset Y-axis position, the calibration operation device shuts down the motion module and gives a calibration failure warning. In this embodiment, in the case where the calibration tooling 100 exceeds the preset Y-axis position, it can be judged that the calibration deviation value of the motion module is relatively large, avoiding the situation that the nucleic acid detection device 200 cannot be used normally due to calibration when the calibration deviation value is relatively large.

[0124] Further, the calibration operation device determines that the current position does not exceed the preset position of the Y-axis and the second detector 4 is set, and marks the current position as the single calibration value.

[0125] In one embodiment, the motion module in this embodiment includes a Y-axis drive, a Z-axis drive, and an S-axis drive. Both the Z-axis drive and the S-axis drive are connected to the pipette tip 210. The Y-axis drive, the Z-axis drive, and the S-axis drive can all adopt drive motors. The Z-axis drive can drive the pipette tip 210 in the up and down direction, and the S-axis drive can drive the pipette tip 210 to rotate in the horizontal plane. The pipette tip 210 can extend into the photoelectric switch of the calibration tooling 100 for photoelectric detection.

[0126] When the calibration operation device determines that the nucleic acid detection device 200 is a device type without an S-axis drive, it controls the motion module to move to the default extraction position of the Y-axis, and controls the motion module to drive the pipetting module along the Z-axis to the default position of the Z-axis. The default position of the Z-axis is equal to the default extraction value of the Z-axis + the calibration range value of the Z-axis. It judges whether the first detector 3 is set, that is, whether the first detector 3 generates a photoelectric detection signal of in-place. When the first detector 3 is at the default position of the Z-axis and is set, the calibration operation device can give a Z-axis calibration out-of-range warning. When the first detector 3 is at the default position of the Z-axis and is not set, the calibration operation device can control the Z-axis drive of the motion module to drive the calibration tooling 100 to move upward step by step with the minimum adjustment distance, and judge that the current position of the motion module does not exceed the preset position of the Z-axis. The preset position of the Z-axis is equal to the default extraction value of the Z-axis - the calibration range value.

[0127] In the embodiment of the present invention, the calibration operation device is further configured to:

[0128] Start timing while performing position calibration;

[0129] Determine that the calibration duration exceeds the preset duration, control the motion module to reset and give a timeout warning.

[0130] The calibration operation device in this embodiment starts calibration timing while controlling the motion module to drive the calibration tooling 100 to perform position calibration, and judges whether the calibration duration exceeds the preset duration. The preset duration in this embodiment can be 5 minutes. When the calibration duration exceeds 5 minutes, a timeout warning is given, and the user can obtain the fault information of the position calibration system as soon as possible.

[0131] Such as Figure 4 and Figure 5As shown, in one embodiment, the calibration operation device, the calibration tooling 100, and the nucleic acid detection device 200 are independent of each other. The calibration operation device is separately provided, and a computer, a notebook, a mobile phone, etc. can be used as the calibration operation device. The calibration operation device may include a control module, a display and interaction module, a storage module, a communication module, and a device identification module. The device identification module can analyze the device type, usage, and historical calibration information of the nucleic acid detection device 200 through the device information reported by the nucleic acid detection device 200. The display and interaction module can display the relevant operation steps and parameters of the current position calibration through the user interface, and can obtain the requirements for the position to be calibrated input by the user through the man-machine interaction method. The storage module is used to preset the default position, the standard parameters for position calibration, and store the historical data of position calibration.

[0132] The calibration tooling 100 further includes a power supply module, a control circuit board, and a communication module. In the process of the calibration operation device in this embodiment controlling the motion module to drive the calibration tooling 100 for position calibration, the display and interaction module can be controlled to display the relevant operation steps and parameters of the current position calibration. The calibration operation device, the calibration tooling 100, and the nucleic acid detection device 200 can be communicatively connected by wireless communication or wired communication.

[0133] The calibration operation device establishes a communication connection with the nucleic acid detection device 200 through a wired or wireless network; taking an external computer as an example, through the Ethernet method, the nucleic acid detection device 200 enters the static IP automatic setting, and then the calibration tooling 100 establishes a communication connection with the calibration operation device through the USB interface (serial port mode) for calibration control through the external computer method. In another embodiment, the calibration operation device is integrated into one of the calibration tooling 100 or the nucleic acid detection device 200.

[0134] In the description of the present invention, it should be understood that the terms "first" and "second" are only used for descriptive purposes, and cannot be understood as indicating or implying relative importance or implicitly indicating the quantity of the indicated technical features. Thus, the features defined with "first" and "second" may explicitly or implicitly include at least one of the features. In the description of the present invention, "a plurality" means at least two, such as two, three, etc., unless otherwise specifically and clearly defined.

[0135] In the present invention, unless otherwise clearly specified and limited, the terms "installation", "connection", "connection", "fixation", etc. should be understood in a broad sense. For example, it can be a fixed connection, a detachable connection, or integrated; it can be a mechanical connection, an electrical connection, or communicable with each other; it can be directly connected, or indirectly connected through an intermediate medium, and can be the internal communication of two components or the interaction relationship between two components, unless otherwise clearly limited. For those of ordinary skill in the art, the specific meanings of the above terms in the present invention can be understood according to specific circumstances.

[0136] In the description of this specification, the descriptions referring to terms such as "one embodiment", "some embodiments", "examples", "specific examples", or "some examples", etc. mean that the specific features, structures, materials, or characteristics described in connection with the embodiment or example are included in at least one embodiment or example of the present invention. In this specification, the schematic representations of the above terms do not necessarily refer to the same embodiment or example. Moreover, the specific features, structures, materials, or characteristics described may be combined in a suitable manner in any one or more embodiments or examples. In addition, without contradiction, those skilled in the art can combine and combine the different embodiments or examples described in this specification and the features of different embodiments or examples.

[0137] Although the embodiments of the present invention have been shown and described above, it can be understood that the above embodiments are exemplary and should not be construed as limiting the present invention. Those of ordinary skill in the art can make changes, modifications, substitutions, and variations to the above embodiments within the scope of the present invention.

Claims

1. A position calibration system for a nucleic acid detection device (200) with a motion module, characterized in that, The position calibration system includes: A calibration tooling (100), adapted to the nucleic acid detection device (200) and used to detect the motion module; A calibration operation device, where both the nucleic acid detection device (200) and the calibration tooling (100) are communicatively connected to the calibration operation device; The calibration operation device is configured to: Send a position calibration instruction to the nucleic acid detection device (200), where the position calibration instruction includes at least a position to be calibrated, and the position to be calibrated includes at least an amplification position and an extraction position; Perform a position calibration operation on the amplification position according to the position calibration instruction, and obtain an amplification calibration value of the amplification position; Perform a position calibration operation on the extraction position according to the position calibration instruction, and obtain an extraction calibration value of the extraction position; Output the amplification calibration value and the extraction calibration value to the nucleic acid detection device (200).

2. The position calibration system according to claim 1, wherein The amplification position includes at least a Y-axis amplification position, and the extraction position includes at least a Y-axis extraction position and a Z-axis extraction position. The calibration operation device is further configured to: Perform a position calibration operation on the Y-axis amplification position according to the position calibration instruction; Perform a position calibration operation on the Y-axis extraction position according to the position calibration instruction; Determine that the calibration of the Y-axis extraction position is successful, and perform a position calibration operation on the Z-axis extraction position according to the position calibration instruction.

3. The position calibration system according to claim 2, characterized in that, The calibration operation device is further configured to: Obtain the default Y-axis extraction position of the Y-axis extraction position; Control the motion module to move to the default Y-axis extraction position; Obtain the default Z-axis position of the Z-axis extraction position; Control the motion module to move to the default Z-axis position; Control the motion module to move along the Z-axis and obtain the current position of the Z-axis extraction position.

4. The position calibration system according to claim 1, characterized in that, The position calibration instruction further includes the number of calibrations. The calibration operation device is further configured to: Loop the position calibration operations of the amplification position and the extraction position according to the number of calibrations; Obtain the single calibration value of the amplification position and the extraction position; Calculate the calibration mean value of the single calibration value.

5. The position calibration system according to claim 4, characterized in that, The calibration operation device is further configured to: Obtain the default value of the position to be calibrated; Determine the maximum offset value and the minimum offset value according to the default value and the single calibration value; Calculate the calibration difference between the maximum offset value and the minimum offset value; Determine that the calibration difference is less than a preset difference, calculate the calibration mean value of the single calibration value and use it as the calibration value.

6. The position calibration system according to any one of claims 1 to 5, characterized in that, An initial position is set on the nucleic acid detection device (200). The calibration operation device is configured to: Determine that the position calibration of the amplification position is successful, and control the motion module to reset to the initial position; Perform the position calibration operation of the extraction position.

7. The position calibration system according to any one of claims 1 to 5, characterized in that, The calibration operation device is further configured to: Obtain the amplification default value and the amplification calibration value of the amplification position; Calculate the amplification offset value of the amplification position; Obtain the extraction default value of the extraction position; Control the motion module to drive the calibration tooling (100) according to the extraction default value and the amplification offset value.

8. The position calibration system according to any one of claims 2 to 5, characterized in that, The calibration tooling (100) includes a profiling main body (1) and a first detector (3) mounted on the profiling main body (1), and the first detector (3) is used to detect the current positions of the Y-axis extraction position and the Z-axis extraction position.

9. The position calibration system according to claim 7, wherein The calibration tooling (100) further includes a position adjustment and measurement member (2) and a second detector (4) mounted on the profiling main body (1). Under the pushing of the nucleic acid detection device (200), the position adjustment and measurement member (2) cooperates with the second detector (4) to obtain the current position of the Y-axis amplification position.

10. The position calibration system according to claim 9, characterized in that, Both the first detector (3) and the second detector (4) are photoelectric switches.

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