Animal cell culture device

Abstract

The invention relates to the technical field of animal cell culture, and discloses an animal cell culture device which comprises a sterile operation room, and an inclined neck bottle is arranged on the lower portion in the sterile operation room. According to the animal cell culture device, the tilting assembly can drive the blowing and beating assembly to integrally tilt and overturn, so that the blowing and beating pipe entering the inclined-neck bottle is also in an inclined state, the inclined state of the inclined-neck bottle enables a culture solution to be concentrated at the corner of the bottom of the bottle, and the inclined state of the blowing and beating pipe facilitates contact with the collected culture solution; the blowing and beating assembly can drive the blowing and beating pipe to draw an elliptical track, the culture solution is blown and beaten along with a pump of the blowing and beating pipe, the sprayed culture solution is used for blowing off cells adhered to the bottle wall, and the elliptical track can enable the blowing and beating pipe not to make contact with the bottle wall when the blowing and beating pipe moves in the inclined-neck bottle. Meanwhile, the blown culture solution is in contact with the bottle wall to the greatest extent, and the blowing and beating actions do not need to be manually carried out, so that the cell culture passage operation speed is greatly increased.

Description

Technical Field

[0001] The invention relates to the technical field of animal cell culture, in particular to an animal cell culture device. Background Art

[0002] Animal cell culture refers to the technology of removing relevant tissues or cells from animals and making them grow, proliferate and maintain certain functions under sterile and suitable in vitro environmental conditions.

[0003] Animal cell culture includes the processes of sampling, primary culture, and subculture. In the process of animal cell culture, a series of equipment and devices are needed. For example, in the primary culture stage, the cell culture bottle is an indispensable key device. It is usually made of transparent, non-toxic materials that can adapt to the growth of cells. The bottle wall is specially treated to facilitate cell attachment and spreading to form a single layer of cells. The culture bottle has different specifications, including flat-bottomed culture bottles and canted-neck culture bottles, especially canted-neck culture bottles, whose neck has a certain inclination angle. The advantage of this design is that it is more convenient to use a pipette to absorb and add liquid during cell subculture operations, which can reduce liquid residue and damage to the cell layer. It can be adjusted according to the number of cultured cells. The bottle stopper is usually designed to be breathable but prevent the entry of external microorganisms, ensuring that the cells are in a relatively stable and sterile culture environment. During subculture, the addition of trypsin digestion solution requires the use of a pipette, which can accurately absorb and add an appropriate amount of digestion solution to detach the adherent cells from the bottle wall to form a cell suspension. In addition, the entire animal cell culture process is inseparable from the clean bench, which can provide a local sterile environment. The air is filtered through a high-efficiency filter inside the bench to keep the air in the operating area clean. Operators perform cell inoculation, fluid replacement and other operations in this environment, greatly reducing the risk of cell contamination.

[0004] The prior art publication number is CN212581937U, which provides an animal cell culture device, including a base, a first cylinder and a liquid storage tank, wherein an air pump and a drain port are installed inside the base, and the upper end of the drain port passes through the base and is connected to the lower surface of the sealing cover, and the upper surface of the base is connected to the lower end of the sealing cover, the lower end of the drain port passes through the rear surface of the base, and an ultraviolet lamp is arranged above the drain port, and the rear surface of the ultraviolet lamp is connected to the inner wall of the sealing cover, a temperature controller is arranged in front of the drain port, and the temperature controller is located in the middle of the upper end of the base, and a bottom plate is arranged above the temperature controller. The animal cell culture device isolates the culture bottle from the outside world through the sealing cover to prevent external microorganisms from entering the culture bottle and causing contamination to the animal cells in the culture bottle, and at the same time adjusts the temperature in the culture bottle through the temperature controller to improve the efficiency of animal culture.

[0005] In the above-mentioned prior art, although the clamping and stirring structures are provided to better mix the cells, it does not have the ability to pipette. The common reagent bottles used for cell culture are inclined-neck bottles. Since the cell culture areas are different, there are different models. However, when subculturing cells, generally, the inclined-neck bottle is placed obliquely manually, and then a pipetting dropper is inserted obliquely. The cell culture medium is sucked, and then the wrist is rotated to make the pipetting dropper draw an arc inside the inclined-neck bottle. The cell culture medium sprays out in an arc, so that the cells adhering to the inner wall of the inclined-neck bottle are no longer adhered and suspended in the culture medium, which is convenient for pouring out, replacing the culture medium or subculturing cells. In the prior art, this pipetting process is all completed manually, with low efficiency and a slow culture operation process.

[0006] It can be seen that an animal cell culture device is needed to solve the problem that in the prior art mentioned in the above background technology, the pipetting operation in the cell culture process is all completed manually, with low efficiency and a slow culture operation process. Summary of the Invention

[0007] The purpose of the present invention is to provide an animal cell culture device to solve the problems raised in the above background technology.

[0008] To solve the above technical problems, the present invention provides the following technical solutions: An animal cell culture device includes a sterile operation room. Below the interior of the sterile operation room, there is an inclined-neck bottle, and on one side of the inclined-neck bottle, there is a cap-twisting assembly for removing and installing the cap of the inclined-neck bottle. Above the interior of the sterile operation room, there is a moving assembly, and below one side of the moving assembly, there is an inclination assembly. On one side of the inclination assembly, there is a pipetting assembly, and the inclination assembly and the pipetting assembly are used to drive the pipetting tube to tilt into the interior of the inclined-neck bottle and make an arc movement; The inclination assembly includes a vertical plate, and on one side of the vertical plate, there is a slope plate. In contact with one side of the slope plate is a rolling block, and the rolling block is hinged at the bottom end of a rotating vertical block. The rotating vertical block can rotate on one side of the vertical plate, and at the rear side of the rolling block hinge, there is a first gear installed on the rotating vertical block. Above the first gear, there is a second gear meshing with it, and the second gear is connected with a rotating clamp through a shaft passing through the rotating vertical block; The rotating clamp is connected to a fixed plate, and at the bottom end of the fixed plate, there is a rotating mounting bracket. Inside the mounting bracket, there are a first bevel gear and a second bevel gear meshing with each other. One end of the transmission shaft extending from the inside of the first bevel gear is connected with a third bevel gear, and the third bevel gear meshes with a fourth bevel gear inside the through bracket. The fourth bevel gear is connected with a clamping block through a shaft, and inside the clamping block, there is a horizontally movable cross bar. One end of the cross bar is hinged with a pipetting tube, and the middle of the pipetting tube passes through a central positioning block and extends below it.

[0009] Preferably, the aseptic operation room includes an operation chamber, and a transparent door is provided on the front side of the operation chamber. An ultraviolet germicidal lamp is installed on the inner wall of the operation chamber, and a plurality of reagent bottles are provided at the bottom end of the operation chamber. A waste liquid collection pool is opened below the inclined neck bottle on one side of the reagent bottle, and a suction device is installed at the rear of the waste liquid collection pool.

[0010] Preferably, the moving assembly includes a main motor, and the main motor drives two longitudinal lead screws to rotate through a pulley group. The two longitudinal lead screws jointly penetrate through the installation frame, and a transverse lead screw is installed inside the installation frame. A moving block is connected to the outside of the transverse lead screw, and a turbine is installed at one end of the transverse lead screw. The bottom end of the moving block is connected to a telescopic rod, and a dropper assembly is provided at the bottom end of the telescopic rod. A worm is meshed below the turbine, and the worm is installed on the outside of a rotating shaft. The rotating shaft is installed at the output end of a sub-motor, and the sub-motor is located on one side of the main motor.

[0011] Preferably, the cap screwing assembly includes a driving motor, and the output end of the driving motor is connected to an inclined disk through a pulley group. A driving gear is connected to the middle position of the inclined disk through a shaft, and a driven gear is meshed above the driving gear. A connecting shaft penetrates through the driven gear, and a clamp is connected to one end of the connecting shaft. The clamp contacts the cap of the inclined neck bottle. A rectangular block is connected to one end of the connecting shaft, and a guiding shaft penetrates through the rectangular block. A ball is connected to the bottom end of the guiding shaft, and the top end of the guiding shaft penetrates through a limiting groove and moves inside it.

[0012] Preferably, the cross-section of the inclined disk is a right trapezoid structure, and the annular surface of the inclined disk contacts the ball. A limiting rectangular block is provided on the outside of the connecting shaft. A limiting rectangular groove matching the connecting shaft is provided inside the driven gear, and a bearing is installed at the end of the connecting shaft in contact with the rectangular block.

[0013] Preferably, the inclined neck bottle has a structure with a hollow interior, a transparent exterior, a thread and a cap at the opening, and a rotating clamp is installed on the outside of the inclined neck bottle, and a moving seat is provided at the bottom end of the rotating clamp.

[0014] Preferably, a moving plate moves at the top end of the vertical plate, and arc-shaped grooves are provided on both sides of the moving plate. An electric telescopic rod is installed at the rear of one side of the moving plate. A first positioning block is installed on one side of the vertical plate, and the first positioning block includes an arc plate, a rocking shaft, and a penetrating shaft. The rocking shaft and the penetrating shaft are respectively arranged at both ends of the arc plate, and the rocking shaft is located inside one of the arc-shaped grooves. The penetrating shaft is positioned on one side of the vertical plate. A second positioning block is provided on the other side of the vertical plate, and the second positioning block has the same structure as the first positioning block. The rotating vertical block is located on one side of the second positioning block, and the penetrating shaft in the second positioning block contacts the rotating vertical block.

[0015] Preferably, one side of the slope plate is provided with a slope surface, and the rolling block rolls on the slope surface. A connecting shaft is arranged inside the middle of the rolling block, and a rotating block is arranged at the end of the connecting shaft. A shaft penetrates through the rotating block, and the shaft is connected to the first gear. The rotating clamp is located above one side of the rotating vertical block.

[0016] Preferably, an arc-drawing motor is installed at the top of the fixing plate, and the output shaft of the arc-drawing motor penetrates through the fixing plate and extends above the mounting frame. The first bevel gear is installed on one side inside the mounting frame, and the second bevel gear is installed at the top inside the mounting frame. A shaft is arranged at the top of the second bevel gear, and the shaft penetrates through the mounting frame and is connected to the fixing plate. A through-frame penetrates through the lower part inside the mounting frame, and the middle position of the through-frame is penetrated by the first bevel gear. A vertical shaft is connected to the middle position of the fourth bevel gear, and the vertical shaft penetrates through the through-frame and extends below it. The vertical shaft is connected to a clamping block, and a bolt penetrates through the outside of the clamping block.

[0017] Preferably, one side of the fixing plate is connected to a cage, and a central positioning block is connected to one side below the cage. An automatic suction pump is installed at the top of the blowing tube, and the blowing tube is of a detachable structure.

[0018] Compared with the prior art, the beneficial effects achieved by the present invention are as follows: First, through the arranged inclination assembly and blowing assembly, the present invention realizes that the inclination assembly drives the whole blowing assembly to tilt and turn, so that the blowing tube entering the oblique-necked bottle is also in an inclined state. The inclined state of the oblique-necked bottle will make the culture medium concentrate in the bottom corner of the bottle. The inclined state of the blowing tube facilitates contacting the collected culture medium. And the blowing assembly drives the blowing tube to draw an elliptical trajectory. As the blowing tube's own pump blows the culture medium, the cells adhered to the bottle wall are blown off by the ejected culture medium. The elliptical trajectory enables the blowing tube to move inside the oblique-necked bottle without contacting the bottle wall, but at the same time maximizes the contact between the blown culture medium and the bottle wall. The blowing action does not require manual operation, greatly improving the speed of cell culture subculture operation.

[0019] Second, through the arranged cap-twisting assembly, when the oblique-necked bottle is placed inside the rotating fixture for clamping, the cap at the front end of the oblique-necked bottle can be removed by starting the cap-twisting assembly. Then, during the process of replacing the culture medium, it is always held by the fixture until the operation is completed, and then the cap is sent to the outside of the oblique-necked bottle and tightened, without manual tightening, strengthening the automation of the device.

[0020] Third, through the arranged aseptic operation room and moving assembly, the present invention realizes that the device operates inside the operation chamber with a sterile environment, and the culture medium inside the oblique-necked bottle can be replaced by using the reagent in the dropper and reagent bottle driven by the moving assembly, which is convenient for operation and greatly improves the liquid replacement speed. BRIEF DESCRIPTION OF THE DRAWINGS

[0021] Figure 1 is a schematic structural diagram of the present invention; Figure 2 is a sectional view of the present invention; Figure 3 is an exploded view of the structure of the moving component of the present invention; Figure 4 is a schematic structural diagram of the moving component of the present invention; Figure 5 is an exploded view of the structure of the cap-tightening component of the present invention; Figure 6 is a schematic structural diagram of the cap-tightening component of the present invention; Figure 7 is an exploded view of the structure of the tilting component of the present invention; Figure 8 is a schematic structural diagram of the tilting component of the present invention; Figure 9 is a schematic structural diagram of the connection between the tilting component and the flushing component of the present invention; Figure 10 is an exploded view of the structure of the flushing component of the present invention; Figure 11 is a schematic structural diagram of the flushing component of the present invention; Figure 12 is a schematic structural diagram of the flushing component of the present invention with the fixing plate removed.

[0022] Among them: 1. Aseptic operation room; 101. Operation chamber; 102. Ultraviolet germicidal lamp; 103. Reagent bottle; 104. Waste liquid collection pool; 2. Moving assembly; 201. Main motor; 202. Longitudinal lead screw; 203. Installation frame; 204. Transverse lead screw; 205. Moving block; 206. Turbine; 207. Telescopic rod; 208. Pipette integration; 209. Sub-motor; 210. Rotating shaft; 211. Worm; 3. Cap screwing assembly; 301. Driving motor; 302. Tilted plate; 303. Driving gear; 304. Driven gear; 305. Clamp; 306. Connecting shaft; 307. Rectangular block; 308. Guide shaft; 309. Ball; 4. Oblique neck bottle; 401. Rotating clamp; 402. Moving seat; 5. Tilted assembly; 501. Vertical plate; 502. Moving plate; 5021. Arc groove; 503. Electric telescopic rod; 504. First positioning block; 5041. Arc plate; 5042. Rocking shaft; 5043. Through shaft; 505. Second positioning block; 506. Rotating vertical block; 507. Rolling block; 508. First gear; 509. Second gear; 510. Rotating clamp; 511. Slope plate; 6. Blowing and beating assembly; 601. Fixed plate; 602. Arc-drawing motor; 603. Mounting frame; 604. First bevel gear; 605. Second bevel gear; 606. Transmission shaft; 607. Third bevel gear; 608. Fourth bevel gear; 609. Through frame; 610. Clamping block; 611. Bolt; 612. Transverse moving rod; 613. Blowing and beating tube; 614. Cage; 615. Central positioning block. Detailed implementation manners

[0023] Next, the technical solutions in the embodiments of the present invention will be clearly and completely described in conjunction with the accompanying drawings in the embodiments of the present invention. Obviously, the described embodiments are only a part of the embodiments of the present invention, rather than all the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by those of ordinary skill in the art without creative efforts shall fall within the protection scope of the present invention.

[0024] Please refer to Figures 1-6 , an animal cell culture device, including an aseptic operation room 1. A slant-neck bottle 4 is arranged below the inside of the aseptic operation room 1, and a cap screwing assembly 3 for removing and installing the cap of the slant-neck bottle 4 is installed on one side of the slant-neck bottle 4.

[0025] In this embodiment, the existing cell culture process is all manually operated in a sterile chamber. The aseptic operation room 1 in this device is equivalent to a sterile chamber. When the device is running, the transparent door outside the device can be closed to prevent bacteria from entering.

[0026] Specifically, the aseptic operation room 1 includes an operation chamber 101, and a transparent door is provided on the front side of the operation chamber 101. An ultraviolet germicidal lamp 102 is installed on the inner wall of the operation chamber 101, and multiple reagent bottles 103 are provided at the bottom end of the operation chamber 101. A waste liquid collection pool 104 is opened on one side of the reagent bottle 103 below the necked flask 4, and a suction device is installed at the rear side of the waste liquid collection pool 104.

[0027] In this embodiment, an aseptic ventilation system can also be equipped inside the aseptic operation room 1 for air exchange. The ultraviolet germicidal lamp 102 can be turned on after the equipment operation ends for sterilization. The reagent bottles 103 inside the operation chamber 101 contain replaced culture medium. Before the experiment starts, the required culture medium is poured into the reagent bottles 103. After one experiment ends, the reagent bottles 103 need to be cleaned or replaced. When the waste liquid inside the waste liquid collection pool 104 is poured out, it will be sucked away by the suction device and transported to the centralized treatment pool. The waste liquid collection pool 104 also needs to be cleaned regularly.

[0028] Specifically, the cap screwing assembly 3 includes a driving motor 301, and the output end of the driving motor 301 is connected to an inclined disk 302 through a pulley group. A driving gear 303 is connected to the middle position of the inclined disk 302 through a shaft, and a driven gear 304 is engaged above the driving gear 303. A connecting shaft 306 penetrates through the driven gear 304, and one end of the connecting shaft 306 is connected to a clamp 305. The clamp 305 contacts the cap of the necked flask 4. One end of the connecting shaft 306 is connected to a rectangular block 307, and a guiding shaft 308 penetrates through the rectangular block 307. A ball 309 is connected to the bottom end of the guiding shaft 308, and the top end of the guiding shaft 308 penetrates through a limiting groove and moves inside it.

[0029] In this embodiment, the driving motor 301 can be a servo motor, equipped with a rotation angle sensing device, which can control the rotation of the inclined disk 302. Since the annular surface of the inclined disk 302 is inclined, the ball 309 rolling on the annular surface will drive the guiding shaft 308 to move. For example, when the ball 309 is at the lowest point of the inclined surface of the inclined disk 302, the guiding shaft 308 will drive the rectangular block 307 to move forward, so that the clamp 305 moves forward closer to the necked flask 4, and then contacts the necked flask 4 to clamp the cap. Then, as the inclined disk 302 continues to rotate, the highest point of the inclined surface will be on one side of the ball 309, and the ball 309 drives the guiding shaft 308 and the rectangular block 307 to move backward, and then drives the clamp 305 to gradually move backward. Combining with the rotation action, it is the action of unscrewing the threaded cap. The clamp 305 holding the cap continues to move backward until it is at the farthest place from the necked flask 4, and then the cap can be temporarily left inside the clamp 305 until the experiment ends.

[0030] Specifically, the cross-section of the swash plate 302 is a right trapezoidal structure, and the annular surface of the swash plate 302 contacts the ball 309. A limiting rectangular block is arranged on the outer side of the connecting shaft 306, and a limiting rectangular groove matching the connecting shaft 306 is arranged inside the driven gear 304. A bearing is installed at the end of the connecting shaft 306 in contact with the rectangular block 307.

[0031] In this embodiment, the capping assembly 3 is installed inside the installation bin. Except for the connecting shaft 306 and the fixture 305 passing through the installation bin, the rest are located inside the installation bin. The components located outside the installation bin need to be cleaned or replaced regularly, while the components located inside the installation bin can be overhauled regularly. Since the connecting shaft 306 and the fixture 305 are both located outside the installation bin, stainless steel can be used to extend the service life. A detachable structure can be adopted between the fixture 305 and the connecting shaft 306 and the driven gear 304 and the rectangular block 307.

[0032] Specifically, the oblique-necked bottle 4 has a structure with a hollow interior, a transparent exterior, threads and a bottle cap at the opening. A rotating fixture 401 is installed on the outer side of the oblique-necked bottle 4, and a moving seat 402 is arranged at the bottom of the rotating fixture 401.

[0033] In this embodiment, due to different culture areas, the oblique-necked bottles 4 have different models. According to the different sizes of the oblique-necked bottles 4, the rotating fixture 401 can be adjusted to firmly clamp them. The rotating fixture 401 has a rotating function, and cooperating with the moving seat 402 below can achieve the action of catering to the inclined pipetting tube 613, enabling the pipetting tube 613 to better enter and exit the interior of the oblique-necked bottle 4. If a subculture experiment needs to be carried out, multiple oblique-necked bottles 4 are required, so the rotating fixture 401 can be set as a multi-station fixture to clamp multiple oblique-necked bottles 4 for convenient subculture experiments.

[0034] Please refer to Figures 1-4 , for the animal cell culture device, a moving component 2 is arranged above the interior of the aseptic operation room 1.

[0035] In this embodiment, the moving component 2 can drive the multiple dropper assemblies 208 to move. Through the settings of the main motor 201 and the auxiliary motor 209, the multiple dropper assemblies 208 can be adjusted longitudinally or horizontally, thereby making it more convenient for the corresponding dropper assemblies 208 to enter the corresponding reagent bottles and making the experiment more convenient.

[0036] Specifically, the moving component 2 includes a main motor 201, and the main motor 201 drives two sets of longitudinal lead screws 202 to rotate through a pulley group. The two sets of longitudinal lead screws 202 penetrate through the mounting frame 203 together. A transverse lead screw 204 is installed inside the mounting frame 203. A moving block 205 is connected to the outside of the transverse lead screw 204. A turbine 206 is installed at one end of the transverse lead screw 204. An expansion link 207 is connected to the bottom end of the moving block 205. A dropper assembly 208 is arranged at the bottom end of the expansion link 207. A worm 211 is engaged below the turbine 206. The worm 211 is installed on the outside of a rotating shaft 210. The rotating shaft 210 is installed at the output end of a sub-motor 209. The sub-motor 209 is located on one side of the main motor 201.

[0037] In this embodiment, the moving component 2 is integrally installed at the top inside the aseptic operation room 1. Both the main motor 201 and the sub-motor 209 are forward and reverse motors. After the main motor 201 is started, it will drive the dropper assembly 208 to move longitudinally. After the sub-motor 209 is started, it will drive the dropper assembly 208 to move horizontally. The cooperation of the two makes the device more flexible. An expansion link 207 is also arranged above the dropper assembly 208, which is convenient for performing operations such as sampling and liquid replacement on reagent bottles of different heights. A suction pump is installed at the top end of the dropper assembly 208, which can suck or discharge the liquid inside the reagent bottle. A plurality of dropper assemblies 208 are detachably connected to the suction pump. The dropper assembly 208 is preferably made of disposable material and is replaced after one use.

[0038] Please refer to Figures 7-12, an animal cell culture device, an inclined component 5 is arranged below one side of the moving component 2, a pipetting component 6 is installed on one side of the inclined component 5, and the inclined component 5 and the pipetting component 6 are used to drive the pipetting tube 613 to incline into the internal part of the Erlenmeyer flask 4 and make an arc movement. The inclined component 5 includes a vertical plate 501, and a slope plate 511 is arranged on one side of the vertical plate 501. A rolling block 507 is in contact with one side of the slope plate 511, and the rolling block 507 is hinged to the bottom end of a rotating vertical block 506. The rotating vertical block 506 can rotate on one side of the vertical plate 501, and a first gear 508 is installed at the rear side of the rotating vertical block 506 where the rolling block 507 is hinged. A second gear 509 is meshed above the first gear 508, and the second gear 509 passes through the rotating vertical block 506 through a shaft and is connected with a rotating clamp 510. The rotating clamp 510 is connected with a fixing plate 601, and a rotating mounting bracket 603 is arranged at the bottom end of the fixing plate 601. A first bevel gear 604 and a second bevel gear 605 which are meshed with each other are arranged inside the mounting bracket 603. One end of a transmission shaft 606 extending from the inside of the first bevel gear 604 is connected with a third bevel gear 607, and the third bevel gear 607 is meshed with a fourth bevel gear 608 inside a through bracket 609. The fourth bevel gear 608 is connected with a clamping block 610 through a shaft, and a transverse moving rod 612 is movably arranged inside the clamping block 610. One end of the transverse moving rod 612 is hinged with a pipetting tube 613, and the middle part of the pipetting tube 613 penetrates through a central positioning block 615 and extends to its lower side.

[0039] In this embodiment, the pipetting action is actually that the suction pump sucks the culture medium inside the Erlenmeyer flask 4 through the pipetting tube 613 and then discharges it. The discharged culture medium contacts the bottle wall, so as to blow off the cells adhered to the bottle wall and make them suspended in the culture medium. The pipetting action is generally used in subculture experiments. After the pipetting action is completed, the culture medium is then migrated by the moving dropper assembly 208 to the inside of another reagent bottle or Erlenmeyer flask for subculture.

[0040] Specifically, a moving plate 502 moves at the top end of the vertical plate 501, and arc-shaped grooves 5021 are formed on both sides of the moving plate 502. An electric telescopic rod 503 is installed at the rear of one side of the moving plate 502. A first positioning block 504 is installed on one side of the vertical plate 501, and the first positioning block 504 includes an arc plate 5041, a rocking shaft 5042, and a through shaft 5043. The rocking shaft 5042 and the through shaft 5043 are respectively arranged at both ends of the arc plate 5041, and the rocking shaft 5042 is located inside one group of arc-shaped grooves 5021. The through shaft 5043 is positioned on one side of the vertical plate 501. A second positioning block 505 is arranged on the other side of the vertical plate 501, and the second positioning block 505 has the same structure as the first positioning block 504. The rotating vertical block 506 is located on one side of the second positioning block 505, and the through shaft in the second positioning block 505 contacts the rotating vertical block 506.

[0041] In this embodiment, when the electric telescopic rod 503 extends, it drives the moving plate 502 to move, thereby driving the first positioning blocks 504 and the second positioning blocks 505 on both sides to flip. The second positioning block 505 drives the rotating vertical block 506 to flip. The flipping angle can be 90 degrees. The rotating angle of the rotating clamp 510 can be 25°, 30° or other appropriate inclination angles. The rotating angle of the rotating clamp 510 is convenient to accommodate the inclination angle of the obliquely necked bottle 4. Since the rotating vertical block 506 will flip 90 degrees, space needs to be reserved for the pipetting tube 613 to flip upward. The general process of inserting the pipetting tube 613 into the obliquely necked bottle 4 is as follows: The obliquely necked bottle 4 is driven by the moving seat 402 to approach the inclination assembly 5. The pipetting tube 613 starts to flip from above to below. At this time, the rotating fixture 401 drives the obliquely necked bottle 4 to rotate and incline, and the pipetting tube 613 that has flipped to below continues to rotate obliquely and enters the interior of the obliquely necked bottle 4. The suction port of the pipetting tube 613 faces the corner where the culture solution in the bottle gathers.

[0042] Specifically, one side of the slope plate 511 is provided with a slope surface, and the rolling block 507 rolls on the slope surface. A connecting shaft is arranged inside the middle of the rolling block 507, and a rotating block is arranged at the end of the connecting shaft. A shaft penetrates through the rotating block, and the shaft is connected to the first gear 508. The rotating clamp 510 is located above one side of the rotating vertical block 506.

[0043] In this embodiment, the flipping of the rotating vertical block 506 causes the rolling block 507 to roll on the slope surface. Due to the slope problem, the shaft penetrating through the middle of the rolling block 507 will rotate at a certain angle. The shaft drives the first gear 508 at the rear to rotate at a certain angle, and then drives the second gear 509 to rotate. The second gear 509 drives the rotating clamp 510 to rotate. When the rotating vertical block 506 flips, the first gear 508 and the second gear 509 at the rear and the rotating clamp 510 at the front will also flip synchronously.

[0044] Specifically, an arc-drawing motor 602 is installed at the top of the fixed plate 601, and the output shaft of the arc-drawing motor 602 penetrates through the fixed plate 601 and extends above the mounting frame 603. The first bevel gear 604 is installed on one side inside the mounting frame 603, and the second bevel gear 605 is installed at the top inside the mounting frame 603. A shaft is arranged at the top of the second bevel gear 605, and the shaft penetrates through the mounting frame 603 and is connected to the fixed plate 601. A through-frame 609 penetrates through the lower part inside the mounting frame 603, and the middle position of the through-frame 609 is penetrated by the first bevel gear 604. A vertical shaft is connected to the middle position of the fourth bevel gear 608, and the vertical shaft penetrates through the through-frame 609 and extends below it. The vertical shaft is connected to a clamping block 610, and a bolt 611 penetrates through the outside of the clamping block 610.

[0045] In this embodiment, the arc-drawing motor 602 can be a servo motor. After starting, the output shaft penetrates through the fixed plate 601 to drive the mounting bracket 603 to rotate. When the mounting bracket 603 rotates, it will drive the first bevel gear 604 mounted on the side wall to revolve. However, the second bevel gear 605 does not move. Due to the meshing with the second bevel gear 605, the first bevel gear 604 will revolve and rotate simultaneously. Therefore, the third bevel gear 607 will revolve and rotate simultaneously. Since the penetrating frame 609 is mounted on the mounting bracket 603, the penetrating frame 609 will also revolve together with the mounting bracket 603. The fourth bevel gear 608 is installed inside the penetrating frame 609. When revolving, due to meshing with the third bevel gear 607, it will also rotate, causing the cross-moving rod 612 to revolve and rotate simultaneously. As a result, an elliptical trajectory can be formed at the end of the cross-moving rod 612. Since the blowing tube 613 extends into the inside of the oblique-necked bottle 4 and the inner cavity of the oblique-necked bottle 4 is a rectangular structure, it meets the elliptical movement trajectory, enabling the blowing tube 613 not to contact the inner wall of the oblique-necked bottle 4 while allowing the liquid to be blown to contact the bottle wall to the greatest extent.

[0046] Specifically, a cage 614 is connected to one side of the fixed plate 601, and a center positioning block 615 is connected to one side below the cage 614. An automatic suction pump is installed at the top of the blowing tube 613, and the blowing tube 613 is a detachable structure.

[0047] In this embodiment, the blowing tube 613 is preferably made of a disposable plastic hose. By starting the automatic suction pump, the culture medium in the oblique-necked bottle 4 can be sucked and discharged. After one experiment, the blowing tube 613 needs to be replaced. The cage 614 installed on one side of the fixed plate 601 does not rotate and will stay relatively fixed in place together with the center positioning block 615. Therefore, both ends of the blowing tube 613 will perform an elliptical movement similar to a mirror image with the point passing through the center positioning block 615 as the center. A limiting structure can be installed at the position where the center of the center positioning block 615 contacts the blowing tube 613, enabling the blowing tube 613 to rotate while the position passing through the center positioning block 615 is relatively positioned. The cross-moving rod 612 can move inside the clamp block 610. Loosening the bolt 611 allows the cross-moving rod 612 to move back and forth inside the clamp block 610, thereby adjusting the size of the arc trajectory. At the extreme points, it can even be adjusted to a circular trajectory, which can be adjusted according to the internal space of the selected reagent bottle, thus enabling the blowing action to be carried out better.

[0048] During use, it is necessary to connect to an external power supply, which provides electrical energy for the device so that the device can operate normally. First, place the slant-neck bottle 4 inside the rotary fixture 401 for clamping. The bottle cap is removed by the cap unscrewing assembly 3. Start the drive motor 301. The drive motor 301 drives the tilt disc 302 to rotate through the pulley group. The tilt disc 302 drives the driving gear 303 to rotate through the shaft. The driving gear 303 drives the driven gear 304 to rotate through the meshing relationship, thereby driving the fixture 305 and the connecting shaft 306 to rotate. The connecting shaft 306 is arranged on one side of the rectangular block 307. When the tilt disc 302 rotates, the ball 309 will roll on the inclined surface. There is a height difference on the inclined surface, so it will drive the guide shaft 308 and the rectangular block 307 to move. The rectangular block 307 causes the connecting shaft 306 to also move, and the fixture 305 will move back and forth while rotating. The fixture 305 clamps the bottle cap, rotates and retreats to disengage it from one side of the slant-neck bottle 4, waits for the experiment to end, then sends out the bottle cap and tightens it on one side of the slant-neck bottle 4; After removing the bottle cap, the experiment can be carried out. If it is necessary to replace the culture solution inside the slant-neck bottle 4, the moving seat 402 can be controlled to rotate first to pour out the original culture solution in the slant-neck bottle 4 into the waste liquid collection pool 104, then rotate to make the mouth of the slant-neck bottle 4 face upward, and then start the main motor 201 and the auxiliary motor 209. The main motor 201 will drive the two groups of longitudinal lead screws 202 to rotate, thereby driving the installation frame 203 to move, so that the dropper assembly 208 moves longitudinally. After the auxiliary motor 209 is started, it will drive the rotary shaft 210 to rotate, thereby driving the worm 211 to rotate. The worm 211 drives the meshing turbine 206 to rotate, and further drives the transverse lead screw 204 to rotate, so that the moving block 205 makes a transverse movement. The lower dropper assembly 208 will also move accordingly. The adjustment in the transverse and longitudinal directions enables the appropriate dropper assembly 208 to enter the corresponding reagent bottle 103 to suck up the new culture solution, and then transport it into the slant-neck bottle 4. The slant-neck bottle 4 receives the new culture solution to complete the replacement, and then the cap is tightened by the cap screwing assembly 3 to complete the liquid replacement operation; If a subculture experiment is required, a pipetting action needs to be added between the experimental steps. Rotate the inclined-neck flask 4 with the bottle cap open to make it inclined, so that the culture medium accumulates at the bottom corner of the inclined-neck flask 4. Start the electric telescopic rod 503, so that the electric telescopic rod 503 pushes the moving plate 502 to move at the top of the vertical plate 501. When the vertical plate 501 moves, the arc-shaped groove 5021 will drive the rocking shaft 5042 to swing, so that the through shaft 5043 rotates. The second positioning block 505 has the same structure as the first positioning block 504, so the same action will also occur. The rotation of the through shaft in the second positioning block 505 drives the rotating vertical block 506 to rotate. When the rotating vertical block 506 rotates, the rolling block 507 below will roll on the slope plate 511. Due to the different slopes, the rolling block 507 will drive the first gear 508 to rotate at a certain angle. The second gear 509 meshing with the first gear 508 will naturally rotate at the same angle, thus driving the rotating clamp 510 to rotate. The rotating clamp 510 is connected to the fixed plate 601, so that the whole pipetting assembly 6 is turned over and inclined at the same time, which is convenient to accommodate the inclined-neck flask 4. Then, the arc-drawing motor 602 is started, and the arc-drawing motor 602 drives the mounting bracket 603 to rotate. The second bevel gear 605 inside the mounting bracket 603 will not rotate, while the first bevel gear 604 will rotate around the second bevel gear 605. The rotation of the first bevel gear 604 drives the third bevel gear 607 to rotate through the transmission shaft 606, and the third bevel gear 607 drives the fourth bevel gear 608 to rotate, thus driving the transverse movement rod 612 to rotate, so that the transverse movement rod 612 rotates while revolving. The end of the pipetting tube 613 at one end of the transverse movement rod 612 will draw an elliptical trajectory. Since it passes through the central positioning block 615 and is relatively positioned in the middle, the other end of the pipetting tube 613 will also make an elliptical trajectory. The pipetting tube 613 moves inside the inclined-neck flask 4 along the elliptical trajectory, and with the start of the suction pump, the cells adhered to the bottle wall are pipetted with the culture medium.

[0049] Although the embodiments of the present invention have been shown and described, for those of ordinary skill in the art, it can be understood that various changes, modifications, substitutions, and variations can be made to these embodiments without departing from the principles and spirit of the present application. The scope of the present application is defined by the appended claims and their equivalents.

Claims

1. An animal cell culture device, comprising a sterile operating room (1), characterized in that: A canted-neck bottle (4) is arranged at the lower part of the aseptic operation room (1), and a cap screwing assembly (3) for removing and installing the cap of the canted-neck bottle (4) is installed on one side of the canted-neck bottle (4); a moving assembly (2) is arranged at the upper part of the aseptic operation room (1), and a tilting assembly (5) is arranged below one side of the moving assembly (2); a blowing assembly (6) is installed on one side of the tilting assembly (5), and the tilting assembly (5) and the blowing assembly (6) are used to drive the blowing tube (613) to tilt into the interior of the canted-neck bottle (4) and make an arc motion; The tilting assembly (5) comprises a vertical plate (501), and a ramp plate (511) is arranged on one side of the vertical plate (501), one side of the ramp plate (511) contacts a rolling block (507), and the rolling block (507) is hinged at the bottom end of a rotating vertical block (506), the rotating vertical block (506) can rotate on one side of the vertical plate (501), and a first gear (508) is installed on the rear side of the rotating vertical block (506) located at the hinge of the rolling block (507), a second gear (509) is meshed above the first gear (508), and the second gear (509) passes through the rotating vertical block (506) through a shaft and is connected to a rotating clamp (510); The rotating clamp (510) is connected to the fixed plate (601), and a rotating mounting frame (603) is provided at the bottom end of the fixed plate (601); a first bevel gear (604) and a second bevel gear (605) meshing with each other are provided on the inner side of the mounting frame (603); one end of a transmission shaft (606) extending from the inside of the first bevel gear (604) is connected to a third bevel gear (607), and the third bevel gear (607) is meshed with a fourth bevel gear (608) on the inner side of a penetration frame (609); the fourth bevel gear (608) is connected to a clamp block (610) via an axis, and a transverse rod (612) is movably provided inside the clamp block (610); a blowing tube (613) is hingedly connected to one end of the transverse rod (612), and the middle part of the blowing tube (613) penetrates the central positioning block (615) and extends to the bottom thereof.

2. The animal cell culture device according to claim 1, characterized in that: The sterile operating room (1) comprises an operating chamber (101), wherein a transparent door is arranged at the front side of the operating chamber (101), an ultraviolet sterilization lamp (102) is installed on the inner wall of the operating chamber (101), and a plurality of groups of reagent bottles (103) are arranged at the bottom end of the operating chamber (101), a waste liquid collection tank (104) is opened on one side of the reagent bottle (103) below the canted-neck bottle (4), and a suction device is installed at the rear side of the waste liquid collection tank (104).

3. The animal cell culture device according to claim 1, characterized in that: The moving assembly (2) comprises a main motor (201), and the main motor (201) drives two groups of longitudinal screw rods (202) to rotate via a pulley group, the two groups of longitudinal screw rods (202) pass through the mounting frame (203), and a transverse screw rod (204) is installed inside the mounting frame (203), a moving block (205) is connected to the outside of the transverse screw rod (204), and a turbine (206) is installed at one end of the transverse screw rod (204), the bottom end of the moving block (205) is connected to a telescopic rod (207), and a dropper assembly (208) is provided at the bottom end of the telescopic rod (207), a worm (211) is meshed below the turbine (206), and the worm (211) is installed on the outside of a rotating shaft (210), and the rotating shaft (210) is installed at the output end of an auxiliary motor (209), and the auxiliary motor (209) is located on one side of the main motor (201).

4. The animal cell culture device according to claim 1, characterized in that: The cap screwing assembly (3) comprises a driving motor (301), wherein the output end of the driving motor (301) is connected to a tilting plate (302) via a pulley group, wherein a driving gear (303) is connected to the middle position of the tilting plate (302) via a shaft, wherein a driven gear (304) is meshed above the driving gear (303), wherein a connecting shaft (306) passes through the interior of the driven gear (304), wherein one end of the connecting shaft (306) is connected to a clamp (305), wherein the clamp (305) contacts the bottle cap of the oblique-neck bottle (4), wherein one end of the connecting shaft (306) is connected to a rectangular block (307), wherein a guide shaft (308) passes through the interior of the rectangular block (307), wherein a ball (309) is connected to the bottom end of the guide shaft (308), wherein a top end of the guide shaft (308) passes through a limiting groove and moves therein.

5. The animal cell culture device according to claim 4, characterized in that: The cross section of the inclined plate (302) is a right-angled trapezoidal structure, and the annular surface of the inclined plate (302) contacts the ball (309). A limited rectangular block is arranged on the outside of the connecting shaft (306). A limited rectangular groove matching the connecting shaft (306) is arranged inside the driven gear (304), and a bearing is installed at the end of the connecting shaft (306) that contacts the rectangular block (307).

6. The animal cell culture device according to claim 1, characterized in that: The oblique-necked bottle (4) is hollow inside, transparent outside, and has a structure with a thread and a bottle cap at the opening. A rotating fixture (401) is installed outside the oblique-necked bottle (4), and a movable seat (402) is provided at the bottom end of the rotating fixture (401).

7. The animal cell culture device according to claim 1, characterized in that: A movable plate (502) is movable at the top of the vertical plate (501), and arc grooves (5021) are provided on both sides of the movable plate (502). An electric telescopic rod (503) is installed at the rear of one side of the movable plate (502). A first positioning block (504) is installed on one side of the vertical plate (501), and the first positioning block (504) comprises an arc plate (5041), a shaking shaft (5042), and a through shaft (5043). The shaking shaft (5042) and the through shaft (5043) are respectively arranged on the circular arc plate (5041), a shaking shaft (5042), and a through shaft (5043). The arc plate (5041) is disposed at both ends thereof, and the rocking shaft (5042) is located inside one group of arc grooves (5021); the through shaft (5043) is located on one side of the vertical plate (501); a second positioning block (505) is provided on the other side of the vertical plate (501); and the second positioning block (505) has the same structure as the first positioning block (504); the rotating vertical block (506) is located on one side of the second positioning block (505), and the through shaft in the second positioning block (505) is in contact with the rotating vertical block (506).

8. The animal cell culture device according to claim 1, characterized in that: A slope surface is provided on one side of the ramp plate (511), and the rolling block (507) rolls on the slope surface. A connecting shaft is provided in the middle of the rolling block (507), and a rotating block is provided at the end of the connecting shaft. A shaft passes through the rotating block, and the shaft is connected to the first gear (508). The rotating clamp (510) is located above one side of the rotating vertical block (506).

9. The animal cell culture device according to claim 1, characterized in that: An arc drawing motor (602) is installed at the top of the fixing plate (601), and the output shaft of the arc drawing motor (602) passes through the fixing plate (601) and extends to the top of the mounting frame (603); the first bevel gear (604) is installed on one side of the mounting frame (603), and the second bevel gear (605) is installed at the top of the mounting frame (603); a shaft is arranged at the top of the second bevel gear (605), and the shaft passes through the mounting frame (603) and is connected to the fixing plate (601); a penetration frame (609) passes through the bottom of the mounting frame (603), and the middle position of the penetration frame (609) is penetrated by the first bevel gear (604); a vertical shaft is connected to the middle position of the fourth bevel gear (608), and the vertical shaft passes through the penetration frame (609) and extends to the bottom thereof; the vertical shaft is connected to a clamping block (610), and a bolt (611) passes through the outer side of the clamping block (610).

10. The animal cell culture device according to claim 1, characterized in that: One side of the fixing plate (601) is connected to a retaining frame (614), and one side below the retaining frame (614) is connected to a center positioning block (615). An automatic suction pump is installed at the top of the blowing tube (613), and the blowing tube (613) is a detachable structure.

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