Bacillus subtilis degradation liquid for northeast walnut oil extraction residues
By using Bacillus subtilis to degrade microorganisms and degrade wild pecan oil extract residues in Northeast China, the problem of difficulty in efficient utilization of these residues is solved, and the effect of efficient degradation and comprehensive utilization is achieved.
Patent Information
- Application Number
- CN202411638920.6
- Authority / Receiving Office
- CN · China
- Patent Type
- Applications(China)
- Current Assignee / Owner
- Filing Date
- 2024-11-07
- Publication Date
- 2025-07-01
AI Technical Summary
There is difficulty in efficient utilization of wild pecan oil extract residues in Northeast China, and the prior art is difficult to effectively degrade these protein-rich residues.
Bacillus subtilis is used as the degradation microorganisms. By mixing the crushed walnut oil residue with Bacillus subtilis fermentation seed solution, appropriate temperature and time control are carried out to promote microbial degradation.
It has achieved efficient degradation of Northeast walnut oil extraction residues and improved the comprehensive utilization value of these residues.
Abstract
Description
Technical Field
[0001] The present invention relates to a method for preparing a Bacillus subtilis degradation solution from the residue of Northeast China walnut oil extraction. Background Art
[0002] Walnuts are rich in protein, the content of which is higher than that of most nuts, and it is an excellent source of protein. The wild walnuts in Changbai Mountain in Northeast China have a rich yield, and the residue after oil extraction is mostly used as feed. Bacillus subtilis is a kind of Bacillus, which is widely distributed in soil and decaying organic matter and is named because it is easy to reproduce in hay infusion. During the growth process of Bacillus subtilis cells, active substances such as subtilin, polymyxin, nystatin, and bacitracin are produced, and the cells of Bacillus subtilis itself synthesize enzymes such as α-amylase, protease, lipase, and cellulase, which are beneficial to the degradation of the residue of Northeast China walnut oil extraction rich in protein. Therefore, we have proposed a method for preparing a Bacillus subtilis degradation solution from the residue of Northeast China walnut oil extraction. Summary of the Invention
[0003] In order to improve the efficient utilization of the residue of wild walnuts in Northeast China for oil extraction, the present invention provides a method for degradation using Bacillus subtilis. Specific Method
[0005] After the residue of Northeast China walnut oil extraction is crushed, it is placed in a sealable container, and sterile water is added under sterile operation, and a fermentation seed solution of commercially available Bacillus subtilis diluted with physiological saline is inoculated. Stir and mix evenly, and place it sealed at an appropriate temperature. The fermentation conditions are as follows: the ratio of material to water is 2:1 - 1:2, the fermentation temperature is 22 - 37 °C, the fermentation time is 1 - 9 days, the concentration of the fermentation seed solution of commercially available Bacillus subtilis is 50 - 100 cfu / mL (the effective bacteria numbers of different preparations are different, so the dosage will also be different. In actual use, please strictly follow the instructions on the product manual), and the inoculation amount is 5 - 20%. Specific Embodiments
[0006] Example 1:
[0007] The preparation method is as follows: 1 kg of the residue of Northeast China walnut oil extraction is crushed and placed in a 10 L sealable container. 1000 mL of sterile water is added under sterile operation, and the ratio of material to water is 1:1. According to the inoculation amount (5%), 50 mL of a commercially available Bacillus subtilis fermentation seed solution diluted with physiological saline and adjusted to a concentration of 50 cfu / mL is inoculated, stirred and mixed evenly, and sealed. The fermentation temperature is 25 °C, and the fermentation time is 3 days. Shake well to obtain a Bacillus subtilis degradation solution prepared from the residue of Northeast China walnut oil extraction.
[0008] The present invention is conducive to the comprehensive utilization of the residue of Northeast China walnut oil extraction.
Claims
1. The Bacillus subtilis degradation liquid of Northeast walnut oil pressing residue is characterized by: The method is to prepare the oil from the residue of walnut oil pressing in Northeast China.
2. The Bacillus subtilis degradation liquid of the Northeastern walnut oil pressing residue is characterized by: The Northeast walnut oil pressing residue is crushed and placed in a sealable container, and commercially available Bacillus subtilis fermentation seed liquid diluted with physiological saline is added, stirred and mixed, and sealed and placed at an appropriate temperature.
3. The Bacillus subtilis degradation liquid of the Northeast walnut oil pressing residue is characterized by: The fermentation conditions are: material-water ratio of 2:1-1:2, fermentation temperature of 22-37 degrees, fermentation time of 1-9 days, commercially available Bacillus subtilis fermentation seed liquid concentration of 50-100 cfu / mL, and inoculation amount of 5-20%.