Sample detection device and sample detection method

Abstract

The invention discloses a sample detection device and a sample detection method. The sample detection device comprises a flow chamber; a flow cell; a liquid delivery module; a light source; the control module is used for controlling the liquid conveying module to convey the sample to the flow chamber when the detection to be performed on the sample is flow fluorescence detection, controlling the light source to emit a detection light beam to generate a first light beam, and receiving an electric signal generated by the first light beam based on the photoelectric sensor to output a detection result; and controlling the liquid conveying module to convey the sample to the flow cell in response to that the detection to be performed on the sample is chemiluminescence detection, so that the sample emits a second light beam in the flow cell, and receiving an electric signal generated by the second light beam based on the photoelectric sensor to output a detection result. Based on the mode, the reliability of the sample detection device can be improved.

Description

Technical Field

[0001] This application relates to the field of detection technology, and particularly to a sample detection device and a sample detection method. Background Art

[0002] Currently, the commonly used immunoassay technology is chemiluminescence technology, including chemiluminescence labeled immunoassay, chemiluminescence enzyme immunoassay, etc. Chemiluminescence detection has the advantages of high detection sensitivity and wide linear range, but chemiluminescence detection can only be used for single-item detection. When multiple immunoassays need to be performed on the same sample, there is a problem of too long detection time. With the development of technology, some manufacturers use flow cytometry fluorescence for multi-item joint immunoassay. Flow cytometry fluorescence detection has the advantages of fast detection speed and low cost, but its sensitivity and linear range are inferior to chemiluminescence detection.

[0003] To meet the detection requirements of different scenarios, testing institutions often need to configure two types of detection instruments, which results in higher detection costs and more cumbersome procedures, leading to poor reliability of existing sample detection devices. Summary of the Invention

[0004] The main technical problem to be solved by this application is how to improve the reliability of the sample detection device.

[0005] To solve the above technical problem, the first technical solution adopted by this application is: A sample detection device, comprising: a flow cell; a flow-through cell; a liquid delivery module for collecting a sample and delivering the sample to the flow cell or the flow-through cell; a light source for emitting a detection beam to the sample in the flow cell to excite the sample to generate a first beam; a photoelectric sensor for receiving the first beam and / or for receiving a second beam emitted by the sample in the flow-through cell; a control module for: in response to the detection to be performed on the sample being flow cytometry fluorescence detection, controlling the liquid delivery module to deliver the sample to the flow cell, controlling the light source to emit a detection beam to generate a first beam, and outputting a detection result based on the electrical signal generated by the photoelectric sensor receiving the first beam; in response to the detection to be performed on the sample being chemiluminescence detection, controlling the liquid delivery module to deliver the sample to the flow-through cell so that the sample emits a second beam in the flow-through cell, and outputting a detection result based on the electrical signal generated by the photoelectric sensor receiving the second beam.

[0006] Among them, the liquid delivery module further includes: a first preparation pipeline for allowing a sample to be detected to enter; a second preparation pipeline for connecting to the first preparation pipeline or the flow cell through a first control valve; the control module is further configured to: control the first control valve to connect the second preparation pipeline and the first preparation pipeline, and control the liquid delivery module to sequentially deliver the sample to the first preparation pipeline and the second preparation pipeline; control the first control valve to disconnect the connection between the first preparation pipeline and the second preparation pipeline, so that part of the sample is intercepted in the first preparation pipeline, and control the first control valve to conduct the connection between the second accurate pipeline and the flow cell, so as to deliver the sample in the second preparation pipeline to the flow cell; the volume of the second preparation pipeline is greater than or equal to the volume of the sample delivered to the flow cell.

[0007] Among them, the liquid delivery module is connected to the second preparation pipeline through a second control valve; the control module is further configured to: in response to the detection to be performed on the sample being chemiluminescence detection, control the first preparation pipeline to aspirate the head-end isolation bubble; control the liquid delivery module to aspirate the sample, so as to sequentially deliver the head-end isolation bubble and the sample to the first preparation pipeline and the second preparation pipeline; before the sample is delivered to the second control valve, control the first control valve to disconnect the connection between the first preparation pipeline and the second preparation pipeline, so that part of the sample is intercepted in the first preparation pipeline, and control the first control valve to conduct the connection between the second accurate pipeline and the flow cell, so as to deliver the sample in the second preparation pipeline to the flow cell; the volume of the second preparation pipeline is greater than the volume of the sample delivered to the flow cell.

[0008] Among them, the first preparation pipeline includes a first branch and a second branch; one end of the second branch is connected to the first control valve, and the other end of the second branch communicates with the first branch and the inner sheath inlet of the flow chamber. The first branch is used for allowing a sample to be detected to enter; the control module is further configured to: in response to the detection to be performed on the sample being flow cytometry fluorescence detection, control the first control valve to conduct the connection between the second preparation pipeline and the first preparation pipeline, and control the liquid delivery module to deliver the sample to the first preparation pipeline; when part of the sample flows through the first control valve, control the first control valve to disconnect the connection between the first preparation pipeline and the second preparation pipeline, so that part of the sample is intercepted in the second preparation pipeline, and control the liquid delivery module to deliver part of the sample in the first branch to the flow chamber.

[0009] Among them, the liquid delivery module includes: a sampling needle; a first switching valve, one end of the first switching valve is connected to the tail of the sampling needle; a first three-way joint, the first port of the first three-way joint is connected to the other end of the first switching valve; a second three-way joint, the first port of the second three-way joint is connected to the second port of the first three-way joint, and the second port of the second three-way joint is connected to the flow cell; a first three-way valve, the second branch port of the first three-way valve is connected to the third port of the second three-way joint, and the first branch port of the first three-way valve is connected to the flow-through cell; a second three-way valve, the first branch port of the second three-way valve is connected to the third port of the first three-way joint, and the second branch port of the second three-way valve is connected to the common port of the first three-way valve; a suction and discharge module, the suction and discharge module is connected to the common port of the second three-way valve, and the suction and discharge module is used for sucking and discharging liquid; controlling the liquid delivery module to deliver the sample to the flow cell includes: controlling the suction and discharge module to suck liquid so that part of the sample is located between the first three-way valve and the second three-way valve, and controlling the suction and discharge module to deliver the sample located between the first three-way valve and the first three-way joint to the flow cell; controlling the liquid delivery module to deliver the sample to the flow-through cell includes: controlling the suction and discharge module to suck liquid so that part of the sample is located between the first three-way valve and the second three-way valve, and controlling the suction and discharge module to deliver the sample located between the first three-way valve and the second three-way valve to the flow-through cell.

[0010] Among them, the second port of the second three-way joint is connected to the sample input port of the flow cell; the suction and discharge module includes a first suction and discharge module and a second suction and discharge module; the liquid delivery module further includes: a third three-way joint, the first port of the third three-way joint is connected to the common port of the second three-way valve, the second port of the third three-way joint is connected to the first suction and discharge module; a second switching valve, one end of the second switching valve is connected to the third port of the third three-way joint; a fourth three-way joint, the first port of the fourth three-way joint is connected to the other end of the second switching valve, the second port of the fourth three-way joint is connected to the sheath liquid input port of the flow cell, and the third port of the fourth three-way joint is connected to the second suction and discharge module.

[0011] Among them, the liquid delivery module further includes: a first reagent module; a second reagent module, the cleaning intensity of the reagent stored in the first reagent module is different from the cleaning intensity of the reagent stored in the second reagent module; a third switching valve, one end of the third switching valve is connected to the first reagent module; a fifth three-way joint, the first port of the fifth three-way joint is connected to the common port of the first three-way valve, the second port of the fifth three-way joint is connected to the second branch port of the second three-way valve, and the third port of the fifth three-way joint is connected to the other end of the third switching valve; a third three-way valve, the common port of the third three-way valve is connected to the first suction and discharge module, the first branch port of the third three-way valve is connected to the second reagent module, and the second branch port of the third three-way valve is connected to the second port of the third three-way joint.

[0012] Wherein, the optical path through which the first light beam is emitted to the photoelectric sensor at least partially overlaps with the optical path through which the second light beam is emitted to the photoelectric sensor; the flow cell is located on the optical path of the first light beam, or a semi-reflective and semi-transmissive lens is provided on the optical path of the first light beam. The semi-reflective and semi-transmissive lens is configured to transmit the first light beam during flow cytometry fluorescence detection so that the photoelectric sensor receives the first light beam, and reflect the second light beam during chemiluminescence detection so that the photoelectric sensor receives the second light beam.

[0013] Wherein, electrodes are provided on both sides of the flow cell, and the electrodes are configured to generate an electric potential, so that chemiluminescence occurs in the sample located in the flow cell; and / or, the bottom of the flow cell is connected to a liquid delivery module, and the liquid delivery module is configured to deliver the sample into the flow cell from the bottom of the flow cell.

[0014] Wherein, the sample detection device further includes: a signal switcher, connected to the photoelectric sensor, and configured to connect the photoelectric sensor to a first circuit or a second circuit; the first circuit is configured to convert the received electrical signal into an analog signal; the second circuit is configured to convert the received electrical signal into a digital signal; the control module is configured to: in response to the detection to be performed on the sample being flow cytometry fluorescence detection, control the signal switcher to connect to the first circuit, and output a detection result based on the analog signal output by the first circuit; in response to the detection to be performed on the sample being chemiluminescence detection, control the signal switcher to connect to the second circuit, and output a detection result based on the digital signal output by the second circuit; the flow chamber, the flow cell, the light source, the photoelectric sensor, and the optical path between the light source and the photoelectric sensor are located in the same dark room.

[0015] To solve the above technical problems, the second technical solution adopted in this application is: a sample detection method applied to the above sample detection device; the sample detection method: in response to the detection to be performed on the sample being flow cytometry fluorescence detection, control the liquid delivery module to deliver the sample to the flow chamber, and control the light source to emit a detection light beam to generate a first light beam, and output a detection result based on the electrical signal generated by the photoelectric sensor receiving the first light beam; in response to the detection to be performed on the sample being chemiluminescence detection, control the liquid delivery module to deliver the sample to the flow cell, so that the sample emits a second light beam in the flow cell, and output a detection result based on the electrical signal generated by the photoelectric sensor receiving the second light beam.

[0016] The beneficial effects of the present application are as follows: Different from the prior art, in the technical solution of the present application, a sample can be transported to a flow cell or a flow-through cell through a liquid delivery module. Among them, the sample in the flow cell can be excited to generate a first light beam for corresponding processing of flow cytometry fluorescence detection to obtain corresponding detection results, and the sample in the flow-through cell can generate chemiluminescence to generate a second light beam for corresponding processing of chemiluminescence detection to obtain corresponding detection results. Based on the above method, it is possible to transport the sample to the flow cell or the flow-through cell respectively through a liquid path transportation method based on the liquid delivery module when the user has different requirements, so as to realize flow cytometry fluorescence detection or chemiluminescence detection. That is, the same sample detection device is enabled to have the ability to perform flow cytometry fluorescence detection and chemiluminescence detection at the same time, so that when the user has different requirements, only the liquid delivery module needs to be controlled to transport the sample to the corresponding position for corresponding sample detection, without separately performing sample detection in two sample detection devices, reducing the detection cost, and the steps are relatively simple, improving the reliability of the sample detection device. BRIEF DESCRIPTION OF THE DRAWINGS

[0017] In order to more clearly illustrate the technical solutions in the embodiments of the present invention, the following will briefly introduce the drawings required for description in the embodiments. Obviously, the drawings in the following description are only some embodiments of the present invention, and those of ordinary skill in the art can obtain other drawings without creative efforts based on these drawings.

[0018] Figure 1 is a schematic structural diagram of an embodiment of the sample detection device of the present application;

[0019] Figure 2 is one of the schematic structural diagrams of an embodiment of the liquid delivery module of the present application;

[0020] Figure 3 is the second of the schematic structural diagrams of an embodiment of the liquid delivery module of the present application;

[0021] Figure 4 is one of the schematic structural diagrams of an embodiment of the optical path system of the present application;

[0022] Figure 5 is the second of the schematic structural diagrams of an embodiment of the optical path system of the present application.

[0023] Reference numerals: flow cell 11, flow-through cell 12, liquid delivery module 13, light source 14, photoelectric sensor 15, control module 16, waste liquid module 17, cleaning swab 18, sampling needle 201, first switching valve 202, first three-way joint 203, second three-way joint 204, first three-way valve 205, second three-way valve 206, aspiration and ejection module 207, first aspiration and ejection module 2071, second aspiration and ejection module 2072, third three-way joint 208, second switching valve 209, fourth three-way joint 210, first reagent module 211, second reagent module 212, third switching valve 213, fifth three-way joint 214, third three-way valve 215, fourth switching valve 216, third reagent module 217, fourth three-way valve 218, fifth three-way valve 219, fifth switching valve 220, waste liquid pump 221. Detailed implementation manners

[0024] The present application will be further described in detail below in conjunction with the accompanying drawings and embodiments. It should be specifically noted that the following embodiments are only used to illustrate the present application, but do not limit the scope of the present application. Similarly, the following embodiments are only partial embodiments of the present application rather than all embodiments. All other embodiments obtained by those of ordinary skill in the art without creative efforts fall within the scope of protection of the present application.

[0025] Referring to "embodiment" herein means that the specific features, structures or characteristics described in conjunction with the embodiment may be included in at least one embodiment of the present application. The phrase appears in various positions in the specification does not necessarily refer to the same embodiment, nor is it an independent or alternative embodiment mutually exclusive with other embodiments. Those skilled in the art will explicitly and implicitly understand that the embodiments described herein may be combined with other embodiments.

[0026] In the description of the present application, it should be noted that unless otherwise clearly specified and limited, the terms "installed", "set", "connected", "connected" should be understood in a broad sense. For example, it may be a fixed connection, a detachable connection, or an integral connection; it may be a mechanical connection or an electrical connection; it may be directly connected, or indirectly connected through an intermediate medium. For those of ordinary skill in the art, the specific meaning of the above terms in the present application can be understood according to specific circumstances.

[0027] The present application provides a sample detection device. Refer to Figure 1 , Figure 1 which is a schematic structural diagram of an embodiment of the sample detection device of the present application. As Figure 1 shown, the sample detection device includes a flow cell 11, a flow-through cell 12, a liquid delivery module 13, a light source 14, a photoelectric sensor 15, and a control module 16.

[0028] The flow cell 11 can be used to form a sheath flow, so that in the sample flow clamped in the middle of the sheath flow, the particles of the sample can flow through one by one for the detection beam emitted by the light source 14 to irradiate one by one.

[0029] The flow-through cell 12 can be used to temporarily store the sample that has undergone chemiluminescence detection processing, so that chemiluminescence occurs in the flow-through cell 12 at this location.

[0030] The liquid delivery module 13 is used to collect the sample and deliver the sample to the flow cell 11 or the flow-through cell 12. Among them, the liquid delivery module 13 can be respectively connected to the flow cell 11 and the flow-through cell 12, and can control the liquid delivery module 13 to collect the sample according to user needs and deliver the collected sample to the flow cell 11 or to the flow-through cell 12.

[0031] The light source 14 is used to emit a detection beam to the sample in the flow cell 11 to excite the sample to generate a first beam. Among them, the light source 14 can specifically be a laser light source or other types of light sources, which is not limited here. By emitting a detection beam to the particles in the flow cell 11, corresponding fluorescence can be excited, that is, a first beam is excited and generated.

[0032] The photoelectric sensor 15 is used to receive the first beam, and / or is used to receive the second beam emitted by the sample in the flow-through cell 12. Among them, the photoelectric sensor 15 can be located on the common optical path of the first beam and the second beam to receive and convert the first beam when it is generated, or receive and convert the second beam when it is generated to generate a corresponding electrical signal, and this electrical signal is used for signal processing to obtain the sample detection result and complete the sample detection.

[0033] The control module 16 is used for:

[0034] In response to the detection to be performed on the sample being flow cytometry fluorescence detection, control the liquid delivery module 13 to deliver the sample to the flow cell 11, control the light source 14 to emit a detection beam to generate a first beam, and output a detection result based on the electrical signal generated by the photoelectric sensor 15 receiving the first beam.

[0035] In response to the detection to be performed on the sample being chemiluminescence detection, control the liquid delivery module 13 to deliver the sample to the flow-through cell 12, so that the sample emits a second beam in the flow-through cell 12, and output a detection result based on the electrical signal generated by the photoelectric sensor 15 receiving the second beam.

[0036] Specifically, when the detection to be performed on the sample is flow cytometry fluorescence detection, control the liquid delivery module 13 to deliver the collected sample to the flow cell 11, so that the detection beam irradiates on the particles of the sample, and corresponding fluorescence beams are excited and generated, denoted as the first beam.

[0037] When the detection to be performed on the sample is chemiluminescence detection, the liquid delivery module 13 is controlled to deliver the collected sample to the flow cell 12, so that the sample processed by chemiluminescence detection located in the flow cell 12 can emit a corresponding luminous beam outward, denoted as the second beam.

[0038] The sensing module 12 includes a photosensitive area, and the photosensitive area is used to receive the first beam or the second beam, and the received first beam or second beam is converted into a corresponding electrical signal by the sensing module 12.

[0039] Optionally, the optical path of the first beam emitted to the sensing module and the optical path of the second beam emitted to the sensing module can form an angle in the photosensitive area; in some embodiments, the optical path of the first beam emitted to the sensing module and the optical path of the second beam emitted to the sensing module can also at least partially overlap.

[0040] Based on the above method, a liquid path system respectively connected to the flow chamber 11 and the flow cell 12 can be constructed through the liquid delivery module 13. Through this liquid path system, when the user needs to perform flow cytometry fluorescence detection or chemiluminescence detection, the sample can be respectively delivered to the modules for corresponding processing. For example, it can be delivered to the flow chamber 11 for optical processing of flow cytometry fluorescence detection, or it can be delivered to the flow cell 12 for chemical processing of chemiluminescence detection, so that the sample emits the corresponding first beam or second beam under different types of sample detections, achieving the technical effect of respectively realizing flow cytometry fluorescence detection and chemiluminescence detection in the same sample detection device, reducing the cost and complexity of the detection steps, and improving the reliability of the sample detection device.

[0041] Different from the prior art, in the technical solution of the present application, a sample can be transported to a flow cell or a flow-through cell through a liquid delivery module. Among them, the sample in the flow cell can be excited to generate a first light beam for corresponding processing of flow cytometry fluorescence detection to obtain corresponding detection results. The sample in the flow-through cell can perform chemiluminescence to generate a second light beam for corresponding processing of chemiluminescence detection to obtain corresponding detection results. Based on the above method, it is possible to, based on the liquid delivery module, through the liquid path transportation method, transport the sample to the flow cell or the flow-through cell respectively under different user requirements, realizing flow cytometry fluorescence detection or chemiluminescence detection. That is, it enables the same sample detection device to simultaneously have the ability to perform flow cytometry fluorescence detection and chemiluminescence detection. Among them, to avoid the short chemiluminescence time affecting the detection, the prior art often first places the reaction cup at the detection position and adds the luminescent substrate at the detection position while performing chemical detection. In the present application, by using a flow-through cell for chemiluminescence detection, in the scenario where a luminescent substrate needs to be added, the liquid to be tested can be quickly output to the flow-through cell through a liquid path delivery device for detection, which is faster than the mechanical cup transfer speed and can meet the detection time requirements of chemiluminescence. It enables the user to only need to control the liquid delivery module to transport the sample to the corresponding position for corresponding sample detection when there are different requirements, without separately performing sample detection in two sample detection devices respectively. Moreover, the flow cytometry fluorescence detection and chemiluminescence detection share a photoelectric sensor, reducing the detection cost and having relatively simple steps, improving the reliability of the sample detection device.

[0042] In one embodiment, referring to Figure 2 and Figure 3 , Figure 2 is one of the schematic structural diagrams of an embodiment of the liquid delivery module of the present application, Figure 3 is the second of the schematic structural diagrams of an embodiment of the liquid delivery module of the present application. As Figure 2 and Figure 3 shown, the liquid delivery module 13 further includes a first preparation pipeline X and a second preparation pipeline Y.

[0043] The first preparation pipeline X is used for the sample to be detected to enter.

[0044] The second preparation pipeline Y is used to be connected to the first preparation pipeline X or the flow-through cell 12 through a first control valve.

[0045] The control module 16 is further used for:

[0046] Controlling the first control valve to conduct the connection between the second preparation pipeline Y and the first preparation pipeline X, and controlling the liquid delivery module 13 to transport the sample to the first preparation pipeline X and the second preparation pipeline Y in sequence.

[0047] Control the first control valve to disconnect the connection between the first preparation pipeline X and the second preparation pipeline Y, so that part of the sample is intercepted in the first preparation pipeline X, and control the first control valve to conduct the connection between the second accurate pipeline and the flow cell 12, so as to transport the sample in the second preparation pipeline Y to the flow cell 12.

[0048] Wherein, the volume of the second preparation pipeline Y is greater than or equal to the volume of the sample transported to the flow cell 12.

[0049] Specifically, the first end of the first preparation pipeline X can be used to collect samples, that is, the sample can enter the first preparation pipeline X from one end of the first preparation pipeline X.

[0050] The second end of the first preparation pipeline X can be connected to the first branch port of the first control valve, one end of the second preparation pipeline Y can be connected to the common port of the first control valve, and the second branch port of the first control valve can be connected to the flow cell. In addition, the third end of the first preparation pipeline X can also be connected to the flow chamber.

[0051] When performing chemiluminescence detection, the connection between the second preparation pipeline Y and the first preparation pipeline X can be conducted, and the liquid delivery module 13 can be controlled to aspirate the sample, so that the sample enters from one end of the first preparation pipeline X and flows through the other end of the first preparation pipeline X, the first control valve, and the first end of the second preparation pipeline Y in sequence. At this time, a part of all the samples entering the first preparation pipeline X and the second preparation pipeline Y is located in the first preparation pipeline X, and the other part is located in the second preparation pipeline Y. The connection between the second preparation pipeline Y and the first preparation pipeline X can be disconnected, so that in all the samples entering the first preparation pipeline X and the second preparation pipeline Y, the tail part that may be affected by air pollution or dilution by diluent or other negative effects is intercepted in the first preparation pipeline X.

[0052] The connection between the second preparation pipeline Y and the flow cell can be conducted, and the sample located in the second preparation pipeline Y can be transported to the flow cell to perform corresponding chemiluminescence and generate a second light beam. By making the volume of the second preparation pipeline Y greater than or equal to the volume of the sample transported to the flow cell 12, it can be ensured that the sample in the second preparation pipeline Y still has enough quantity to be transported to the flow cell after the tail part of the intercepted sample is introduced into the second preparation pipeline Y.

[0053] Based on the above method, the possibility of performing chemiluminescence detection using contaminated samples can be reduced, and it can be ensured that a sufficient amount of samples can be transported to the flow cell for chemiluminescence detection, improving the reliability of the sample detection device.

[0054] Optionally, the power source of the liquid delivery module 13 is connected to the second preparation pipeline Y through a second control valve.

[0055] The control module 16 is further used for:

[0056] In response to the detection to be performed on the sample being chemiluminescence detection, control the first preparation pipeline X to aspirate the head-end isolation bubble.

[0057] Control the liquid delivery module 13 to aspirate the sample, so as to sequentially deliver the head-end isolation bubble and the sample to the first preparation pipeline X and the second preparation pipeline Y.

[0058] Before the sample is delivered to the second control valve, control the first control valve to disconnect the connection between the first preparation pipeline X and the second preparation pipeline Y, so that part of the sample is intercepted in the first preparation pipeline X, and control the first control valve to conduct the connection between the second accurate pipeline and the flow cell 12, so as to deliver the sample in the second preparation pipeline Y to the flow cell 12.

[0059] Wherein, the volume of the second preparation pipeline Y is greater than the volume of the sample delivered to the flow cell 12.

[0060] Specifically, the second end of the second preparation pipeline Y can be connected to the first end of the second control valve, and the second end of the second control valve can be connected to the liquid delivery module 13.

[0061] When the detection to be performed on the sample is chemiluminescence detection and the sample needs to be delivered to the flow cell, the control module 16 can first aspirate a section of head-end isolation bubble and then aspirate the sample.

[0062] When the sample passes through the first preparation pipeline X and the second preparation pipeline Y in sequence and arrives before the second control valve at the second end of the second preparation pipeline Y, the connection between the first preparation pipeline X and the second preparation pipeline Y can be disconnected, and the connection between the second preparation pipeline Y and the flow cell can be conducted, so as to deliver the sample in the second preparation pipeline Y to the flow cell to perform chemiluminescence and generate a second light beam.

[0063] Based on the above method, by aspirating a section of head-end isolation bubble before aspirating the sample, it is possible to isolate the diluent or other liquid originally present in the first preparation pipeline X from the sample through the head-end isolation bubble, and avoid the influence of diffusion contamination on the head end of the sample.

[0064] By disconnecting the connection between the first preparation pipeline X and the second preparation pipeline Y before the sample arrives at the second control valve at the second end of the second preparation pipeline Y, so that part of the sample is intercepted in the first preparation pipeline X, the possibly contaminated part at the tail end of the sample can be discarded; and it is possible to avoid the sample entering the second control valve and causing contamination to the pipeline after the second control valve, resulting in a problem of difficult cleaning.

[0065] Optionally, in some embodiments, the volume of the second preparation pipeline is greater than or equal to the sum of the volume of the sample delivered to the flow cell and the volume of the head-end isolation bubble. Through this design, it can be ensured that the sample to be delivered to the flow cell can be completely stored in the second preparation pipeline and separated from the second control valve by the head-end isolation bubble, avoiding the sample from entering the second control valve and the pipeline after the second control valve, resulting in contamination of the power source and the reagent pipeline, which requires a complex cleaning process for cleaning, and ensuring the reliability of the sample detection device.

[0066] Based on the above method, through the treatment of bubble isolation and partial interception, the possibility that the sample finally input into the flow cell is still contaminated can be reduced as much as possible, improving the accuracy of sample detection.

[0067] Optionally, the first preparation pipeline X includes a first branch and a second branch. One end of the second branch is connected to the first control valve, and the other end of the second branch communicates with the first branch and the inner sheath inlet of the flow chamber 11. The first branch is used for the sample to be detected to enter.

[0068] The control module 16 is further configured to:

[0069] In response to the detection to be performed on the sample being flow cytometry fluorescence detection, control the first control valve to conduct the connection between the second preparation pipeline Y and the first preparation pipeline X, and control the liquid delivery module 13 to deliver the sample to the first preparation pipeline X.

[0070] When a part of the sample flows through the first control valve, control the first control valve to disconnect the connection between the first preparation pipeline X and the second preparation pipeline Y, so that a part of the sample is intercepted in the second preparation pipeline Y, and control the liquid delivery module 13 to deliver a part of the sample in the first branch to the flow chamber 11.

[0071] Specifically, when performing flow cytometry fluorescence detection, the connection between the second preparation pipeline Y and the first preparation pipeline X can be conducted, and the liquid delivery module 13 can be controlled to aspirate the sample, so that the sample enters from one end of the first preparation pipeline X and sequentially flows through the other end of the first preparation pipeline X, the first control valve, and the first end of the second preparation pipeline Y. At this time, a part of all the samples entering the first preparation pipeline X and the second preparation pipeline Y is located in the first preparation pipeline X, and the other part is located in the second preparation pipeline Y. The connection between the second preparation pipeline Y and the first preparation pipeline X can be disconnected, so that the head-end part of all the samples entering the first preparation pipeline X and the second preparation pipeline Y, which may be diluted by the diluent originally present in the pipeline, is intercepted in the second preparation pipeline Y. Then, control the liquid delivery module 13 to deliver the sample still existing in the first branch of the first preparation pipeline X to the flow chamber for the processing of flow cytometry fluorescence detection to generate the first light beam.

[0072] Based on the above method, the possibility of using diluted samples for flow cytometry detection can be reduced, and the reliability of the sample detection device is improved.

[0073] In the sample detection device provided in the above embodiment, the flow cell and the flow-through cell can share the sample preparation pipeline and the control valve, and are adapted to provide different control methods during sample delivery for two different detection methods, so as to ensure the pollution-free detection of samples in both detection modes while sharing the liquid path, and improve the reliability of the sample detection device while reducing costs.

[0074] In one embodiment, as Figure 2 and Figure 3 shown, the liquid delivery module 13 includes a sampling needle 201, a first switching valve 202, a first three-way joint 203, a second three-way joint 204, a first three-way valve 205, a second three-way valve 206, and a suction and discharge module 207.

[0075] One end of the first switching valve 202 is connected to the tail of the sampling needle 201.

[0076] The first port of the first three-way joint 203 is connected to the other end of the first switching valve 202.

[0077] The first port of the second three-way joint 204 is connected to the second port of the first three-way joint 203, and the second port of the second three-way joint 204 is connected to the flow cell 11.

[0078] The second branch port of the first three-way valve 205 is connected to the third port of the second three-way joint 204, and the first branch port of the first three-way valve 205 is connected to the flow-through cell 12.

[0079] The first branch port of the second three-way valve 206 is connected to the third port of the first three-way joint 203, and the second branch port of the second three-way valve 206 is connected to the common port of the first three-way valve 205.

[0080] The suction and discharge module 207 is connected to the common port of the second three-way valve 206, and the suction and discharge module 207 is used for sucking and discharging liquid.

[0081] Among them, controlling the liquid delivery module 13 to deliver the sample to the flow cell 11 includes: controlling the suction and discharge module 207 to suck liquid so that part of the sample is located between the first three-way valve 205 and the second three-way valve 206, and controlling the suction and discharge module to deliver the sample located between the first three-way valve 205 and the first three-way joint 203 to the flow cell 11.

[0082] Controlling the liquid delivery module 13 to deliver the sample to the flow-through cell 12 includes: controlling the suction and discharge module 207 to suck liquid so that part of the sample is located between the first three-way valve 205 and the second three-way valve 206, and controlling the suction and discharge module to deliver the sample located between the first three-way valve 205 and the second three-way valve 206 to the flow-through cell 12.

[0083] Specifically, as Figure 2 and Figure 3 shown, based on the above structure, the pipeline between the first three-way joint 203 and the first three-way valve 205 can be the first preparation pipeline X described in the previous embodiment, the pipeline between the first three-way valve 205 and the second three-way valve 206 can be the second preparation pipeline Y described in the previous embodiment, the first three-way valve 205 can specifically be the first control valve described in the previous embodiment, and the second three-way valve 206 can specifically be the second control valve described in the previous embodiment.

[0084] Specifically, when the control module 16 controls the liquid delivery module 13 to deliver the sample to the flow cell 11, it can control the suction and discharge module 207 to suck the liquid, so that part of the sample is located between the first three-way valve 205 and the second three-way valve 206. That is, it realizes the technical effect of intercepting the first end part that may be diluted or contaminated by the diluent originally present in the pipeline among all the samples entering the first preparation pipeline X and the second preparation pipeline Y in the previous embodiment in the second preparation pipeline Y, reducing the possibility of the sample delivered to the flow cell 11 subsequently being diluted or contaminated, and improving the accuracy of flow cytometry fluorescence detection.

[0085] Specifically, when the control module 16 controls the liquid delivery module 13 to deliver the sample to the flow-through cell 12, it can also control the suction and discharge module 207 to suck the liquid, so that part of the sample is located between the first three-way valve 205 and the second three-way valve 206. That is, it realizes the technical effect of discarding the part that may be affected by contamination at the tail end of the sample among all the samples entering the first preparation pipeline X and the second preparation pipeline Y in the previous embodiment, reducing the possibility of the sample delivered to the flow cell 11 subsequently being contaminated, and improving the accuracy of flow cytometry fluorescence detection.

[0086] Optionally, as Figure 2 and Figure 3 shown, the second port of the second three-way joint 204 is connected to the sample input port of the flow cell 11.

[0087] The suction and discharge module 207 includes a first suction and discharge module 2071 and a second suction and discharge module 2072.

[0088] The liquid delivery module 13 further includes a third three-way joint 208, a second switching valve 209, and a fourth three-way joint 210.

[0089] The first port of the third three-way joint 208 is connected to the common port of the second three-way valve 206, and the second port of the third three-way joint 208 is connected to the first suction and discharge module 2071.

[0090] One end of the second switching valve 209 is connected to the third port of the third three-way joint 208.

[0091] The first port of the fourth three-way joint 210 is connected to the other end of the second switching valve 209. The second port of the fourth three-way joint 210 is connected to the sheath liquid input port of the flow cell 11. The third port of the fourth three-way joint 210 is connected to the second suction and discharge module 2072.

[0092] Specifically, it should be noted that the first suction and discharge module 2071 may specifically be composed of one or more power sources with suction and discharge capabilities. One or more power sources with suction and discharge capabilities can be connected through corresponding valves to form the first suction and discharge module 2071 for performing corresponding suction and discharge operations. In addition, the second suction and discharge module 2072 may also specifically be composed of one or more power sources with suction and discharge capabilities. One or more power sources with suction and discharge capabilities can be connected through corresponding valves to form the second suction and discharge module 2072 for performing corresponding suction and discharge operations, which is not limited herein. The power source may specifically be a syringe or other equipment with suction and discharge capabilities, which is not limited herein.

[0093] In the embodiment of the present application, an example is given in which the first suction and discharge module 2071 is composed of one power source and the second suction and discharge module 2072 is composed of one power source.

[0094] As Figure 2 and Figure 3 shown, when the second switching valve 209 is opened, the second three-way valve 206 conducts the connection between its second branch port and its common port, and the first three-way valve 205 conducts the connection between its first branch port and its common port, the first suction and discharge module 2071 and the second suction and discharge module 2072 can jointly push the sample located between the first three-way valve 205 and the second three-way valve 206 into the flow cell 12 to improve the pushing efficiency.

[0095] The second suction and discharge module 2072 can also deliver sheath liquid to the flow cell 11 through the second port of the fourth three-way joint 210 to form a sheath flow.

[0096] Based on the above method, it can not only ensure sufficient power for sample delivery to the vicinity of the flow cell 12, but also utilize the second suction and discharge module 2072 to provide a sheath flow, improve the utilization rate of the suction and discharge module, and reduce the cost of the suction and discharge module.

[0097] Furthermore, the liquid delivery module 13 further includes a first reagent module 211, a second reagent module 212, a third switching valve 213, a fifth three-way joint 214, and a third three-way valve 215.

[0098] The cleaning intensity of the reagent stored in the first reagent module 211 is different from the cleaning intensity of the reagent stored in the second reagent module 212.

[0099] One end of the third switching valve 213 is connected to the first reagent module 211.

[0100] The first port of the fifth three-way joint 214 is connected to the common port of the first three-way valve 205, the second port of the fifth three-way joint 214 is connected to the second branch port of the second three-way valve 206, and the third port of the fifth three-way joint 214 is connected to the other end of the third switching valve 213.

[0101] The common port of the third three-way valve 215 is connected to the first suction and discharge module 2071, the first branch port of the third three-way valve 215 is connected to the second reagent module 212, and the second branch port of the third three-way valve 215 is connected to the second port of the third three-way joint 208.

[0102] Specifically, by setting corresponding valves on the liquid path connected to the first suction and discharge module 2071, and setting the first reagent module 211 and the second reagent module 212 storing different cleaning intensities, after the sample is transported to the flow cell 11 for flow cytometry fluorescence detection, the reagent with a lower cleaning intensity in the first reagent module 211 and the second reagent module 212 can be extracted to clean the pipeline through which the sample passes. After the sample is transported to the flow-through cell 12 for chemiluminescence detection, the reagent with a higher cleaning intensity in the first reagent module 211 and the second reagent module 212 can be extracted to clean the pipeline through which the sample passes. Since the sample used in chemiluminescence detection usually contains more reagents that are difficult to clean, based on the above method, after chemiluminescence detection, a reagent with a higher cleaning intensity can be used to clean the pipeline to improve the cleaning effect, and during flow cytometry fluorescence detection, a reagent with a lower cleaning intensity and lower cost can be used to clean the pipeline, reducing the cost.

[0103] In one example, the cleaning intensity of the reagent stored in the first reagent module 211 may be greater than the cleaning intensity of the reagent stored in the second reagent module 212.

[0104] Furthermore, as Figure 2 and Figure 3 shown, the sample detection device may further include a waste liquid module 17 and a cleaning swab 18.

[0105] The liquid delivery module 13 may include a fourth switching valve 216, a third reagent module 217, a fourth three-way valve 218, a fifth three-way valve 219, a fifth switching valve 220, and a waste liquid pump 221.

[0106] The flow-through cell 12 is connected to the waste liquid module 17.

[0107] The flow cell 11 is connected to one end of the fourth switching valve 216, and the other end of the fourth switching valve 216 is connected to the waste liquid module 17.

[0108] The third reagent module 217 is connected to the first branch port of the fourth three-way valve 218. The second branch port of the fourth three-way valve 218 is connected to the common port of the fifth three-way valve 219. The common port of the fourth three-way valve 218 is connected to the second suction and discharge module 2072.

[0109] The first branch port of the fifth three-way valve 219 is connected to the third port of the fourth three-way joint 210. The second branch port of the fifth three-way valve 219 is connected to one end of the fifth switching valve 220. The other end of the fifth switching valve 220 is connected to the liquid inlet of the cleaning swab 18. The liquid outlet of the cleaning swab 18 is connected to the waste liquid pump 221. The waste liquid pump 221 is also connected to the waste liquid module 17.

[0110] Specifically, by way of example, first, when performing flow cytometry fluorescence detection, the specific control process of the control module 16 can be as follows:

[0111] Control the needle tip of the sampling needle 201 to enter below the liquid level of the container containing the sample.

[0112] Control the third three-way valve 215 to conduct its second branch port and its common port, the second three-way valve 206 to conduct its second branch port and the common port, the first three-way valve 205 to conduct its second branch port and its common port, and the first switching valve 202 to open.

[0113] Control the first suction and discharge module 2071 to suck liquid so that a part of the sample is between the first switching valve 202 and the first three-way valve 205, and a part of the sample is between the first three-way valve 205 and the second three-way valve 206. Control the first three-way valve 205 to conduct its first branch port and its common port.

[0114] Control the fourth three-way valve 218 to conduct its second branch port and its common port, the fifth three-way valve 219 to conduct its second branch port and its common port, the fifth switching valve 220 to open, and control the second suction and discharge module 2072 to discharge liquid so as to discharge the cleaning liquid into the cleaning space of the cleaning swab 18 to clean the outer wall of the sampling needle in the cleaning space. The waste liquid generated after cleaning can be pumped by the waste liquid pump 221 into the waste liquid module 17. Among them, the cleaning liquid can specifically be the cleaning liquid extracted from the third reagent module 217 when the fourth three-way valve 218 conducts its first branch port and its common port.

[0115] Control the first switching valve 202 to close, the first three-way valve 205 to conduct its first port to its common port, the second three-way valve 206 to conduct its first port to its common port, and the fifth three-way valve 219 to conduct its first port to its common port. Control the second suction and discharge module 2072 to discharge liquid, and open the fourth switching valve 216, so that a part of the liquid flow formed by the discharge of the second suction and discharge module 2072 enters the flow cell 11 to form a sheath flow, and the outflowing sheath liquid passes through the fourth switching valve 216 and enters the waste liquid module 17, while the other part enters the liquid path between the first three-way joint 203 and the second three-way joint 204 from the second three-way valve 206 to form a thrust force to transport the sample in the liquid path between the first three-way joint 203 and the second three-way joint 204 to the vicinity of the inner sheath orifice of the flow cell. After that, control the first suction and discharge module 2071 to discharge liquid, and similarly transport the liquid flow formed by its discharge into the liquid path between the first three-way joint 203 and the second three-way joint 204 from the second three-way valve 206 to form a thrust force to transport the sample in the liquid path between the first three-way joint 203 and the second three-way joint 204 to the vicinity of the inner sheath orifice of the flow cell.

[0116] After 0.5 seconds or other preset duration, control the second switching valve 209 to close, and control the first suction and discharge module 2071 and the second suction and discharge module 2072 to continue discharging liquid, so that the first suction and discharge module 2071 pushes the sample in the liquid path between the first three-way joint 203 and the second three-way joint 204 into the flow cell, and the second suction and discharge module 2072 inputs sheath liquid into the flow cell 11 to form a sheath flow. At this time, the sample in the flow cell can be processed to generate a first light beam for flow cytometry fluorescence detection. After the flow cytometry fluorescence detection is completed, the first suction and discharge module 2071 can be controlled to stop discharging liquid, and the second suction and discharge module 2072 continues to discharge liquid, so that the sample in the flow cell is pushed back into the liquid path between the first three-way joint 203 and the second three-way joint 204 by the sheath flow.

[0117] Control the first suction and discharge module 2071 to suck liquid, and the second suction and discharge module 2072 to continue discharging liquid, so that all the samples in the flow cell are pushed back into the liquid path between the first three-way joint 203 and the second three-way joint 204. Control the first suction and discharge module 2071 to stop discharging liquid, and control the third three-way valve 215 to conduct its first port to its common port. Control the first switching valve 202 to open, and control the second suction and discharge module 2072 to continue discharging liquid, so that a part of the cleaning liquid continuously discharged from the second suction and discharge module 2072 in the flow cell flows into the waste liquid module 17 through the waste liquid pump 221, and the other part passes through the liquid path between the first three-way joint 203 and the second three-way joint 204 and is spit into the corresponding waste liquid bucket or the waste liquid module 17 from the sampling needle 201. Control the first switching valve 202 to close and the second suction and discharge module 2072 to discharge liquid, so that the cleaning liquid only flows into the waste liquid module 17 through the waste liquid pump 221 to thoroughly clean the inner wall of the flow cell.

[0118] Control the first switching valve 202 to open and the fourth switching valve 216 to close, and control the second suction and discharge module 2072 to discharge liquid, so that the cleaning liquid flows from the flow chamber to the liquid path between the first three-way joint 203 and the second three-way joint 204, and then is discharged from the sampling needle 201 into the corresponding waste liquid bucket or waste liquid module 17.

[0119] Control the first three-way valve 205 to conduct its second branch port to its common port, the second three-way valve 206 to conduct its second branch port to its common port, and the second switching valve 209 to open, and control the second suction and discharge module 2072 to discharge liquid, so that the cleaning liquid cleans the pipeline between the first three-way valve 205 and the second three-way joint 204, and the waste liquid is discharged from the sampling needle 201 into the corresponding waste liquid bucket or waste liquid module 17.

[0120] Control the first three-way valve 205 to conduct its first branch port to its common port, the second three-way valve 206 to conduct its first branch port to its common port, and control the second suction and discharge module 2072 to discharge liquid, so that the cleaning liquid cleans the pipeline between the second three-way valve 206 and the first three-way joint 203, and the waste liquid is discharged from the sampling needle 201 into the corresponding waste liquid bucket or waste liquid module 17.

[0121] Complete the preparatory work, detection work and cleaning work for flow cytometry fluorescence detection.

[0122] Second, when performing chemiluminescence detection, the specific control process of the control module 16 can be as follows:

[0123] Control the needle tip of the sampling needle 201 to enter above the liquid level of the container containing the sample.

[0124] Control the fifth three-way valve 219 to conduct its first branch port to its common port, the fourth three-way valve 218 to conduct its second branch port to its common port, the second switching valve 209 to open, the second three-way valve 206 to conduct its second branch port to its common port, the first three-way valve 205 to conduct its second branch port to its common port, and the first switching valve 202 to open, and control the second suction and discharge module 2072 to suck liquid, so that the sampling needle 201 sucks the head-end isolation bubble.

[0125] Control the needle tip of the sampling needle 201 to enter below the liquid level of the container containing the sample.

[0126] Control the second suction and discharge module 2072 to suck liquid, so that the sample enters the pipeline from the sampling needle 201, and then part of the sample is between the first switching valve 202 and the first three-way valve 205, and part of the sample is between the first three-way valve 205 and the second three-way valve 206.

[0127] Control the first switching valve 202 to close and the first three-way valve 205 to conduct its first branch port to its common port, control the second suction and discharge module 2072 to discharge liquid. After all the samples between the first three-way valve 205 and the second three-way valve 206 enter the flow cell, control the second suction and discharge module 2072 to stop discharging liquid. At this time, the samples can be processed for chemiluminescence detection in the flow cell to generate a second light beam.

[0128] After completing the chemiluminescence detection, control the second suction and discharge module 2072 to discharge liquid so that the cleaning liquid cleans the liquid path between the flow cell and the second suction and discharge module 2072, and the waste liquid flows into the waste liquid module 17 connected to the flow cell.

[0129] Control the first switching valve 202 to open and the fourth switching valve 216 to close, control the second suction and discharge module 2072 to discharge liquid so that the cleaning liquid flows from the flow chamber to the liquid path between the first three-way joint 203 and the second three-way joint 204, and then is discharged from the sampling needle 201 into the corresponding waste liquid bucket or waste liquid module 17.

[0130] Control the first three-way valve 205 to conduct its second branch port to its common port, the second three-way valve 206 to conduct its second branch port to its common port, and the second switching valve 209 to open, control the second suction and discharge module 2072 to discharge liquid so that the cleaning liquid cleans the pipeline between the first three-way valve 205 and the second three-way joint 204, and the waste liquid is discharged from the sampling needle 201 into the corresponding waste liquid bucket or waste liquid module 17.

[0131] Control the first three-way valve 205 to conduct its first branch port to its common port, the second three-way valve 206 to conduct its first branch port to its common port, control the second suction and discharge module 2072 to discharge liquid so that the cleaning liquid cleans the pipeline between the second three-way valve 206 and the first three-way joint 203, and the waste liquid is discharged from the sampling needle 201 into the corresponding waste liquid bucket or waste liquid module 17.

[0132] Complete the preparatory work, detection work and cleaning work for chemiluminescence detection.

[0133] Third, when performing chemiluminescence detection, to achieve strong cleaning, after the above-mentioned completion of chemiluminescence detection, change to execute the following steps:

[0134] Control the first three-way valve 205 to conduct its second branch port to its common port, open the third switching valve 213, and control the second suction and discharge module 2072 to suck liquid, so that the cleaning liquid with a higher cleaning intensity in the first reagent module 211 is stored in the pipeline between the fifth three-way joint 214 and the second three-way valve 206. Control the first three-way valve 205 to conduct its first branch port to its common port, close the third switching valve 213, and control the second suction and discharge module 2072 to discharge liquid, so that the cleaning liquid stored in the pipeline between the fifth three-way joint 214 and the second three-way valve 206 cleans the flow cell, and the waste liquid flows into the waste liquid module 17 connected to the flow cell. Control the first three-way valve 205 to conduct its second branch port to its common port, open the first switching valve 202, and control the needle port of the sampling needle 201 to move to the cleaning space of the cleaning swab 18, and control the second suction and discharge module 2072 to discharge liquid, so that the cleaning liquid stored in the pipeline between the fifth three-way joint 214 and the second three-way valve 206 cleans the pipeline between the first three-way valve 205 and the sampling needle 201, and discharges the waste liquid into the waste liquid module 17 through the cleaning swab 18 and its connected waste liquid pump 221.

[0135] Control the first three-way valve 205 to conduct its second branch port to its common port. Control the second suction and discharge module 2072 to discharge liquid, so that the cleaning liquid cleans the liquid path between the flow cell and the second suction and discharge module 2072, and the waste liquid flows into the waste liquid module 17 connected to the flow cell.

[0136] Control the first switching valve 202 to open and the fourth switching valve 216 to close, and control the second suction and discharge module 2072 to discharge liquid, so that the cleaning liquid goes from the flow chamber to the liquid path between the first three-way joint 203 and the second three-way joint 204, and then is discharged from the sampling needle 201 into the corresponding waste liquid bucket or the waste liquid module 17.

[0137] Control the first three-way valve 205 to conduct its second branch port to its common port, the second three-way valve 206 to conduct its second branch port to its common port, and the second switching valve 209 to open, and control the second suction and discharge module 2072 to discharge liquid, so that the cleaning liquid cleans the pipeline between the first three-way valve 205 and the second three-way joint 204, and the waste liquid is discharged from the sampling needle 201 into the corresponding waste liquid bucket or the waste liquid module 17.

[0138] Control the first three-way valve 205 to conduct its first branch port to its common port, the second three-way valve 206 to conduct its first branch port to its common port, and control the second suction and discharge module 2072 to discharge liquid, so that the cleaning liquid cleans the pipeline between the second three-way valve 206 and the first three-way joint 203, and the waste liquid is discharged from the sampling needle 201 into the corresponding waste liquid bucket or the waste liquid module 17.

[0139] Complete the preparatory work, detection work, and cleaning work for chemiluminescence detection.

[0140] In one embodiment, referring to Figure 4 and Figure 5 , Figure 4 is one of the schematic structural diagrams of an embodiment of the optical path system of the present application, Figure 5 is the second of the schematic structural diagrams of an embodiment of the optical path system of the present application. As Figure 4 and Figure 5 shown, the light source 14 may include light source A1 and light source A2, and the photoelectric sensor 15 may include sensor B1 and sensor B2.

[0141] The optical path of the first beam emitted to the photoelectric sensor 15 at least partially overlaps with the optical path of the second beam emitted to the photoelectric sensor 15.

[0142] Specifically, as Figure 4 and Figure 5 shown, the overlapping optical path C is the partial optical path where the first beam and the second beam overlap before entering the photoelectric sensor 15. By making at least part of the optical paths of the first beam and the second beam overlap before entering the photoelectric sensor 15, it is possible to receive the two beams through the same sensor in the photoelectric sensor 15. That is, it is possible to make the same sensor receive different beams during flow cytometry fluorescence detection and chemiluminescence detection respectively to achieve multiplexing, improve the utilization rate of the photoelectric sensor 15, reduce costs, and also reduce the volume of the sample detection device, further improving the reliability of the sample detection device.

[0143] Optionally, as Figure 5 shown, the flow cell 12 is located on the optical path of the first beam.

[0144] Or,

[0145] as Figure 4 shown, a semi-reflective and semi-transmissive lens D is provided on the optical path of the first beam. The semi-reflective and semi-transmissive lens D is used for: when the detection mode of the sample is flow cytometry fluorescence detection, transmitting the first beam so that the photoelectric sensor 15 receives the first beam. When the detection mode of the sample is chemiluminescence detection, reflecting the second beam so that the photoelectric sensor 15 receives the second beam.

[0146] Specifically, by directly arranging the flow cell 12 on the optical path of the first beam, both the first beam generated by flow cytometry fluorescence detection and the second beam generated by chemiluminescence detection can enter the sensor of the same photoelectric sensor 15, and photoelectric conversion is performed to generate corresponding electrical signals to obtain the detection result.

[0147] It is also possible to set a semi-reflective and semi-transmissive lens D at the intersection point of the optical path of the first light beam and the direction of the second light beam emitted when the reaction vessel reaches the flow cell 12, for reflecting the second light beam onto the optical path where the first light beam is emitted to the photoelectric sensor 15, so that both the first light beam generated by flow cytometry fluorescence detection and the second light beam generated by chemiluminescence detection can enter the sensor of the same photoelectric sensor 15, realizing photoelectric conversion to generate corresponding electrical signals to obtain the detection result.

[0148] Based on the above method, by directly or indirectly emitting the second light beam into the photoelectric sensor 15 into which the first light beam is emitted, it is possible to receive two light beams through the same sensor in the photoelectric sensor 15, that is, it can make the same sensor receive different light beams respectively during flow cytometry fluorescence detection and chemiluminescence detection to achieve multiplexing, improving the utilization rate of the photoelectric sensor 15, reducing costs, and also being able to reduce the volume of the sample detection device, further improving the reliability of the sample detection device.

[0149] In one embodiment, electrodes are provided on both sides of the flow cell 12, and the electrodes are used to generate an electric potential to cause the sample located in the flow cell 12 to produce a chemiluminescence phenomenon. And / or,

[0150] The bottom of the flow cell 12 is connected to a liquid delivery module 13, and the liquid delivery module 13 is used to deliver the sample into the flow cell 12 from the bottom of the flow cell 12.

[0151] Specifically, based on the above method, it is possible to realize chemiluminescence detection of electrochemiluminescence by generating electricity through the electrodes, or perform chemiluminescence detection of direct luminescence or indirect luminescence when the electrodes do not generate electricity, improving the functionality of sample detection.

[0152] In addition, by connecting the liquid delivery module 13 to the bottom of the flow cell 12, when there are bubbles in the liquid delivered by the liquid delivery module 13 to the flow cell 12, they can be quickly discharged upward from the bottom of the flow cell 12, reducing the possibility of the delivery path being blocked by bubbles and improving the reliability of the sample detection device.

[0153] In one embodiment, the sample detection device further includes:

[0154] A signal switcher, connected to the photoelectric sensor 15, for connecting the photoelectric sensor 15 to a first circuit or a second circuit.

[0155] The first circuit is used to convert the received electrical signal into an analog signal.

[0156] The second circuit is used to convert the received electrical signal into a digital signal.

[0157] The control module 16 is used for:

[0158] In response to the detection to be performed on the sample being flow cytometry fluorescence detection, the control signal switcher connects to the first circuit and outputs the detection result based on the analog signal output by the first circuit.

[0159] In response to the detection to be performed on the sample being chemiluminescence detection, the control signal switcher connects to the second circuit and outputs the detection result based on the digital signal output by the second circuit.

[0160] Among them, the flow cell 11, the flow-through cell 12, the light source 14, the photoelectric sensor 15, and the optical path between the light source 14 and the photoelectric sensor 15 are located in the same darkroom.

[0161] Specifically, the first circuit is used to convert the received electrical signal into an analog signal. Among them, the first circuit can be used to convert the electrical signal output by the photoelectric sensor 15 after photoelectric conversion into an analog signal. It should be noted that when performing flow cytometry fluorescence detection, the electrical signal is usually converted into an analog signal for subsequent processing and analysis to ensure that the accuracy of the corresponding detection result is high enough.

[0162] The second circuit is used to convert the received electrical signal into a digital signal. Among them, the second circuit can be used to convert the electrical signal output by the photoelectric sensor 15 after photoelectric conversion into a digital signal. It should be noted that when performing chemiluminescence detection, the electrical signal is usually converted into a digital signal for subsequent processing and analysis to ensure that the accuracy of the corresponding detection result is high enough.

[0163] Based on the above method, by connecting the same photoelectric sensor 15 to the signal switcher and connecting the first circuit for converting the analog signal and the second circuit for converting the digital signal behind the signal switcher, it is possible to switch to the most suitable branch respectively when performing flow cytometry fluorescence detection and chemiluminescence detection, thereby improving the accuracy of the detection results in different types of detection modes obtained by subsequent signal processing of the electrical signal output by the photoelectric sensor 15. That is, it enables the sample detection device to share the same photoelectric sensor for flow cytometry fluorescence detection and chemiluminescence detection, making the function of the sample detection device diversified, avoiding the situation where two or more sample detection devices or photoelectric receivers corresponding to flow cytometry fluorescence detection and chemiluminescence detection respectively are required to perform flow cytometry fluorescence detection and chemiluminescence detection separately, reducing the cost and improving the reliability of the sample detection device.

[0164] In the above embodiments, chemiluminescence detection is performed by setting up the flow cell 12, which enables the flow chamber 11, the flow cell 12, the light source 14, the photoelectric sensor 15, and the optical path between the light source 14 and the photoelectric sensor 15 to be located in the same dark room. Compared with chemiluminescence detection using reaction cups, there is no need to set up an additional cup transfer mechanism and a door opening / closing mechanism for the dark room, which can reduce the setting of the dark room, improve the anti-interference ability of the optical path, avoid the negative impact of external light on the optical path where the first light beam or the second light beam is located, and improve the reliability of the sample detection device.

[0165] It should be noted that flow cytometry fluorescence detection may include the detection of at least one classified fluorophore. That is, in a single flow cytometry fluorescence detection, only one classified fluorophore can be detected, or two or more classified fluorophores can be detected simultaneously.

[0166] To achieve the simultaneous detection of two or more classified fluorophores, a quantitative fluorescence receiver and at least one classified fluorescence receiver, as well as a quantitative light source and at least one classified light source, can be set in the sample detection device. The quantitative light source can be used to emit a light beam to the sample to excite and generate corresponding fluorescence beams, and the quantitative fluorescence receiver is used to receive the fluorescence beams corresponding to the quantitative light source. The classified light source can be used to emit a light beam to the sample to excite and generate corresponding fluorescence beams, and the classified fluorescence receiver is used to receive the fluorescence beams corresponding to the corresponding classified light source. Then, based on the electrical signals respectively converted and generated by the quantitative fluorescence receiver and at least one classified fluorescence receiver, the results of flow cytometry fluorescence detection of two or more classified fluorophores are obtained.

[0167] Optionally, two or more fluorescence beams can be excited and generated by the classified light sources respectively corresponding to two or more classified fluorophores, or two or more fluorescence beams respectively corresponding to different classified fluorophores can be excited and generated by one classified light source for two or more classified fluorescence receivers to receive.

[0168] Optionally, the sensor shared when performing flow cytometry fluorescence detection and chemiluminescence detection can specifically be the receiver with the best performance among the quantitative fluorescence receiver and at least one classified fluorescence receiver. The fluorescence beam received by the sensor with the best performance is the above-mentioned first light beam.

[0169] This application also proposes a sample detection method, which is applied to the sample detection device described in any of the above embodiments.

[0170] The sample detection method includes:

[0171] Step S11: In response to the detection to be performed on the sample being flow cytometry fluorescence detection, control the liquid delivery module 13 to deliver the sample to the flow cell 11, control the light source 14 to emit a detection beam to generate a first beam, and output a detection result based on the electrical signal generated by the optoelectronic sensor 15 receiving the first beam.

[0172] Step S12: In response to the detection to be performed on the sample being chemiluminescence detection, control the liquid delivery module 13 to deliver the sample to the flow-through cell 12, so that the sample emits a second beam in the flow-through cell 12, and output a detection result based on the electrical signal generated by the optoelectronic sensor 15 receiving the second beam.

[0173] Step S11 and step S12 are in a parallel relationship and are executed alternatively. For example, step S11 is executed when the detection to be performed on the sample is flow cytometry fluorescence detection, and step S12 is executed when the detection to be performed on the sample is chemiluminescence detection.

[0174] In one embodiment, in response to the detection to be performed on the sample being flow cytometry fluorescence detection in step S11, controlling the liquid delivery module 13 to deliver the sample to the flow cell 11 includes:

[0175] In response to the detection to be performed on the sample being chemiluminescence detection, control the first control valve to conduct the connection between the second preparation pipeline and the first preparation pipeline, and control the liquid delivery module 13 to deliver the sample to the first preparation pipeline and the second preparation pipeline in sequence.

[0176] Control the first control valve to disconnect the connection between the first preparation pipeline and the second preparation pipeline, so that part of the sample is intercepted in the first preparation pipeline, and control the first control valve to conduct the connection between the second accurate pipeline and the flow-through cell 12 to deliver the sample in the second preparation pipeline to the flow-through cell 12.

[0177] Wherein, the volume of the second preparation pipeline is greater than or equal to the volume of the sample delivered to the flow-through cell 12.

[0178] In one embodiment, in response to the detection to be performed on the sample being flow cytometry fluorescence detection in step S11, controlling the liquid delivery module 13 to deliver the sample to the flow cell 11 includes:

[0179] In response to the detection to be performed on the sample being chemiluminescence detection, control the first preparation pipeline to aspirate the head-end isolation bubble.

[0180] Control the liquid delivery module 13 to aspirate the sample to deliver the head-end isolation bubble and the sample to the first preparation pipeline and the second preparation pipeline in sequence.

[0181] Before the sample is delivered to the second control valve, control the first control valve to disconnect the first preparation pipeline from the second preparation pipeline, so that part of the sample is intercepted in the first preparation pipeline, and control the first control valve to connect the second accurate pipeline to the flow cell 12 to deliver the sample in the second preparation pipeline to the flow cell 12.

[0182] In one embodiment, in response to the detection to be performed on the sample being chemiluminescence detection, controlling the liquid delivery module 13 to deliver the sample to the flow cell 12 includes:

[0183] In response to the detection to be performed on the sample being flow cytometry fluorescence detection, control the first control valve to connect the second preparation pipeline to the first preparation pipeline, and control the liquid delivery module 13 to deliver the sample to the first preparation pipeline.

[0184] When part of the sample flows through the first control valve, control the first control valve to disconnect the first preparation pipeline from the second preparation pipeline, so that part of the sample is intercepted in the second preparation pipeline, and control the liquid delivery module 13 to deliver part of the sample in the first branch to the flow chamber 11.

[0185] In one embodiment, the sample detection device further includes:

[0186] A signal switcher, connected to the photoelectric sensor, for connecting the photoelectric sensor to the first circuit or the second circuit.

[0187] The first circuit is used to convert the received electrical signal into an analog signal.

[0188] The second circuit is used to convert the received electrical signal into a digital signal.

[0189] The sample detection method further includes:

[0190] In response to the detection to be performed on the sample being flow cytometry fluorescence detection, control the signal switcher to connect to the first circuit, and output the detection result based on the analog signal output by the first circuit.

[0191] In response to the detection to be performed on the sample being chemiluminescence detection, control the signal switcher to connect to the second circuit, and output the detection result based on the digital signal output by the second circuit.

[0192] Specifically, the sample detection method may include the steps performed by the control module described in any of the foregoing embodiments, which will not be elaborated here.

[0193] Different from the prior art, in the technical solution of the present application, a sample can be transported to a flow cell or a flow-through cell through a liquid delivery module. Among them, the sample in the flow cell can be excited to generate a first light beam for corresponding processing of flow cytometry fluorescence detection to obtain corresponding detection results. The sample in the flow-through cell can generate chemiluminescence to generate a second light beam for corresponding processing of chemiluminescence detection to obtain corresponding detection results. Based on the above method, it is possible to transport the sample to the flow cell or the flow-through cell respectively in the case of different user requirements through the liquid delivery module by means of liquid path transportation, so as to realize flow cytometry fluorescence detection or chemiluminescence detection. That is, the same sample detection device can simultaneously have the ability to perform flow cytometry fluorescence detection and chemiluminescence detection, so that the user only needs to control the liquid delivery module to transport the sample to the corresponding position for corresponding sample detection when there are different requirements, without separately performing sample detection in two sample detection devices, reducing the detection cost, and the steps are relatively simple, improving the reliability of the sample detection device.

[0194] In the description of the present application, the descriptions referring to terms such as "one embodiment", "some embodiments", "example", "specific example", or "some examples" etc. mean that the specific features, structures, materials or characteristics described in connection with the embodiment or example are included in at least one embodiment or example of the present application. In this specification, the schematic representations of the above terms do not necessarily refer to the same embodiment or example. Moreover, the specific features, structures, materials or characteristics described can be combined in a suitable manner in any one or more embodiments or examples. In addition, without contradiction, those skilled in the art can combine and combine the different embodiments or examples described in this specification and the features of different embodiments or examples.

[0195] In addition, the terms "first" and "second" are only used for descriptive purposes and cannot be understood as indicating or implying relative importance or implicitly specifying the quantity of the indicated technical features. Thus, the features defined with "first" and "second" can explicitly or implicitly include at least one of the features. In the description of the present application, the meaning of "a plurality" is at least two, such as two, three, etc., unless otherwise clearly and specifically defined.

[0196] Any process or method description shown in the flowchart or described in other ways herein can be understood as representing a module, segment, or part of code including one or more executable instructions for implementing a specific logical function or process. The scope of the preferred embodiments of the present application includes additional implementations, where the functions can be executed in a substantially simultaneous manner or in a reverse order according to the involved functions, rather than in the order shown or discussed, which should be understood by those skilled in the art of the embodiments of the present application.

[0197] The logic and / or steps represented in the flowchart or otherwise described herein, for example, can be considered as a definitional sequence list of executable instructions for implementing logical functions, and can be specifically implemented in any computer-readable medium for use by an instruction execution system, apparatus, or device (which can be a personal computer, server, network device, or other system that can fetch instructions from the instruction execution system, apparatus, or device and execute the instructions), or in combination with these instruction execution systems, apparatus, or devices. For the purposes of this specification, a "computer-readable medium" can be any device that can contain, store, communicate, propagate, or transport a program for use by or in combination with an instruction execution system, apparatus, or device. More specific examples (non-exhaustive list) of computer-readable media include the following: an electrical connection portion having one or more wirings (electronic device), a portable computer diskette (magnetic device), a random access memory (RXM), a read-only memory (ROM), an erasable programmable read-only memory (EPROM or flash memory), an optical fiber device, and a portable compact disc read-only memory (CDROM). Additionally, the computer-readable medium can even be paper or other suitable media on which the program can be printed, because the program can be obtained electronically, for example, by optically scanning the paper or other media, followed by editing, interpretation, or otherwise processing as appropriate, and then storing it in a computer memory.

[0198] The above are only the embodiments of the present application, and do not limit the patent scope of the present application. Any equivalent structure or equivalent process transformation made by using the content of the specification and drawings of the present application, or directly or indirectly applied in other related technical fields, shall be equally included in the patent protection scope of the present application.

Claims

1. A sample detection device, characterized in that, Comprising: Flow cell; Flow-through cell; Liquid delivery module for collecting a sample and delivering the sample to the flow cell or the flow-through cell; Light source for emitting a detection beam to the sample in the flow cell to excite the sample to generate a first beam; Photoelectric sensor for receiving the first beam and / or for receiving a second beam emitted by the sample in the flow-through cell; Control module for: In response to the detection to be performed on the sample being flow cytometry fluorescence detection, controlling the liquid delivery module to deliver the sample to the flow cell, controlling the light source to emit the detection beam to generate the first beam, and outputting a detection result based on the electrical signal generated by the photoelectric sensor receiving the first beam; In response to the detection to be performed on the sample being chemiluminescence detection, controlling the liquid delivery module to deliver the sample to the flow-through cell so that the sample emits the second beam in the flow-through cell, and outputting a detection result based on the electrical signal generated by the photoelectric sensor receiving the second beam.

2. The sample detection device according to claim 1, characterized in that, The liquid delivery module further comprises: First preparation pipeline for the sample to be detected to enter; Second preparation pipeline for being connected to the first preparation pipeline or the flow-through cell through a first control valve; The control module is further used for: Controlling the first control valve to conduct the connection between the second preparation pipeline and the first preparation pipeline, and controlling the liquid delivery module to sequentially deliver the sample to the first preparation pipeline and the second preparation pipeline; Controlling the first control valve to disconnect the connection between the first preparation pipeline and the second preparation pipeline so that part of the sample is intercepted in the first preparation pipeline, and controlling the first control valve to conduct the connection between the second preparation pipeline and the flow-through cell to deliver the sample in the second preparation pipeline to the flow-through cell; Wherein, the volume of the second preparation pipeline is greater than or equal to the volume of the sample delivered to the flow-through cell.

3. The sample detection device according to claim 2, wherein The power source of the liquid delivery module is connected to the second preparation pipeline through a second control valve; The control module is further used for: In response to the detection to be performed on the sample being chemiluminescence detection, controlling the first preparation pipeline to suck the head-end isolation bubble; Controlling the liquid delivery module to suck the sample to sequentially deliver the head-end isolation bubble and the sample to the first preparation pipeline and the second preparation pipeline; Before the sample is delivered to the second control valve, controlling the first control valve to disconnect the connection between the first preparation pipeline and the second preparation pipeline so that part of the sample is intercepted in the first preparation pipeline, and controlling the first control valve to conduct the connection between the second preparation pipeline and the flow-through cell to deliver the sample in the second preparation pipeline to the flow-through cell; Wherein, the volume of the second preparation pipeline is greater than the volume of the sample delivered to the flow-through cell.

4. The sample detection device according to claim 2, wherein, The first preparation pipeline includes a first branch and a second branch; one end of the second branch is connected to the first control valve, and the other end of the second branch communicates with the first branch and the inner sheath inlet of the flow chamber. The first branch is used for the sample to be detected to enter; The control module is further configured to: In response to the detection to be performed on the sample being a flow cytometry fluorescence detection, control the first control valve to conduct the connection between the second preparation pipeline and the first preparation pipeline, and control the liquid delivery module to deliver the sample to the first preparation pipeline; When a part of the sample flows through the first control valve, control the first control valve to disconnect the connection between the first preparation pipeline and the second preparation pipeline, so that a part of the sample is intercepted in the second preparation pipeline, and control the liquid delivery module to deliver a part of the sample in the first branch to the flow chamber.

5. The sample detection device according to any one of claims 1 to 4, characterized in that, The liquid delivery module includes: A sampling needle; A first switching valve, one end of the first switching valve is connected to the tail of the sampling needle; A first three-way joint, the first port of the first three-way joint is connected to the other end of the first switching valve; A second three-way joint, the first port of the second three-way joint is connected to the second port of the first three-way joint, and the second port of the second three-way joint is connected to the flow chamber; A first three-way valve, the second branch port of the first three-way valve is connected to the third port of the second three-way joint, and the first branch port of the first three-way valve is connected to the flow cell; A second three-way valve, the first branch port of the second three-way valve is connected to the third port of the first three-way joint, and the second branch port of the second three-way valve is connected to the common port of the first three-way valve; A suction and discharge module, the suction and discharge module is connected to the common port of the second three-way valve, and the suction and discharge module is used for sucking and discharging liquid; Wherein, controlling the liquid delivery module to deliver the sample to the flow chamber includes: controlling the suction and discharge module to suck liquid so that a part of the sample is located between the first three-way valve and the second three-way valve, and controlling the suction and discharge module to deliver the sample between the first three-way valve and the first three-way joint to the flow chamber; Controlling the liquid delivery module to deliver the sample to the flow cell includes: controlling the suction and discharge module to suck liquid so that a part of the sample is located between the first three-way valve and the second three-way valve, and controlling the suction and discharge module to deliver the sample between the first three-way valve and the second three-way valve to the flow cell.

6. The sample detection device according to claim 5, characterized in that, The second port of the second three-way joint is connected to the sample input port of the flow chamber; The suction and discharge module includes a first suction and discharge module and a second suction and discharge module; The liquid delivery module further includes: A third three-way joint, the first port of the third three-way joint is connected to the common port of the second three-way valve, and the second port of the third three-way joint is connected to the first suction and discharge module; A second switching valve, one end of the second switching valve is connected to the third port of the third three-way joint; Fourth three-way joint, the first port of the fourth three-way joint is connected to the other end of the second switching valve, the second port of the fourth three-way joint is connected to the sheath liquid input port of the flow chamber, and the third port of the fourth three-way joint is connected to the second suction and discharge module.

7. The sample detection device according to claim 6, wherein, The liquid delivery module further includes: First reagent module; Second reagent module, the cleaning intensity of the reagent stored in the first reagent module is different from the cleaning intensity of the reagent stored in the second reagent module; Third switching valve, one end of the third switching valve is connected to the first reagent module; Fifth three-way joint, the first port of the fifth three-way joint is connected to the common port of the first three-way valve, the second port of the fifth three-way joint is connected to the second branch port of the second three-way valve, and the third port of the fifth three-way joint is connected to the other end of the third switching valve; Third three-way valve, the common port of the third three-way valve is connected to the first suction and discharge module, the first branch port of the third three-way valve is connected to the second reagent module, and the second branch port of the third three-way valve is connected to the second port of the third three-way joint.

8. The sample detection device according to any one of claims 1 to 4, characterized in that The optical path of the first light beam emitted to the photoelectric sensor at least partially overlaps with the optical path of the second light beam emitted to the photoelectric sensor; Wherein, the flow cell is located on the optical path of the first light beam, or A semi-reflective and semi-transmissive lens is provided on the optical path of the first light beam, and the semi-reflective and semi-transmissive lens is used to transmit the first light beam during flow cytometry fluorescence detection so that the photoelectric sensor receives the first light beam, and to reflect the second light beam during chemiluminescence detection so that the photoelectric sensor receives the second light beam.

9. The sample detection device according to any one of claims 1 to 4, characterized in that Electrodes are provided on both sides of the flow cell, and the electrodes are used to generate an electric potential so that the sample located in the flow cell generates a chemiluminescence phenomenon; and / or, The bottom of the flow cell is connected to the liquid delivery module, and the liquid delivery module is used to transport the sample into the flow cell from the bottom of the flow cell.

10. The sample detection device according to any one of claims 1 to 4, characterized in that, The sample detection device further includes: Signal switch, connected to the photoelectric sensor, for connecting the photoelectric sensor to the first circuit or the second circuit; The first circuit is used to convert the received electrical signal into an analog signal; The second circuit is used to convert the received electrical signal into a digital signal; The control module is used for: In response to the detection to be performed on the sample being flow cytometry fluorescence detection, controlling the signal switch to connect to the first circuit, and outputting the detection result based on the analog signal output by the first circuit; In response to the detection to be performed on the sample being chemiluminescence detection, controlling the signal switch to connect to the second circuit, and outputting the detection result based on the digital signal output by the second circuit; Wherein, the flow chamber, the flow cell, the light source, the photoelectric sensor, and the optical path between the light source and the photoelectric sensor are located in the same dark room.

11. A sample detection method, characterized in that, Applied to the sample detection device according to any one of claims 1 to 10; The sample detection method includes: In response to the detection to be performed on the sample being a flow cytometry fluorescence detection, control the liquid delivery module to deliver the sample to the flow chamber, control the light source to emit the detection beam to generate the first beam, and output a detection result based on the electrical signal generated by the first beam received by the photoelectric sensor; In response to the detection to be performed on the sample being a chemiluminescence detection, control the liquid delivery module to deliver the sample to the flow cell, so that the sample emits the second beam in the flow cell, and output a detection result based on the electrical signal generated by the second beam received by the photoelectric sensor.

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