Identification method and application of high-temperature-resistant grape variety

Abstract

The invention relates to the field of grape variety identification, in particular to a high-temperature-resistant grape variety identification method which comprises the following steps: step 1, grape sample selection: selecting different grape fruit and leaf samples; 2, high-temperature stress treatment: setting high-temperature-resistant varieties as an experimental group, and setting common varieties as a control group; treating the experimental group and the control group in a high-temperature incubator respectively to obtain a treated experimental group and a treated control group respectively; 3, measuring physiological and biochemical indexes: respectively putting the treated experimental group and the control group into a grape variety measuring device for measuring the physiological and biochemical indexes of grapes to obtain the physiological and biochemical indexes of the experimental group and the physiological and biochemical indexes of the control group; 4, result judgment: comparing the physiological and biochemical indexes of the experimental group with the physiological and biochemical indexes of the control group, and judging the high-temperature resistance of the high-temperature-resistant variety. According to the method, the high-temperature-resistant grape variety can be efficiently and accurately identified.

Description

Technical Field

[0001] The present invention relates to the field of grape variety identification, and specifically relates to a method and application for identifying heat-tolerant grape varieties. Background Art

[0002] Grape (Vitis vinifera L.) is one of the most widely cultivated fruit trees in the world. During its growth and development, it will inevitably suffer from various biotic or abiotic stresses. With the continuous increase in the global average temperature, heat stress has gradually become the main adversity factor restricting the development of the grape industry. When grapes mature, it coincides with high-temperature weather in summer (>35°C). Continuous heat stress will trigger a series of physiological and biochemical changes in grape plants, affecting the fluidity and permeability of cell membranes, causing disorders in the structure and function of thylakoid membranes, and inducing redox reactions, responses of stress-resistant genes, and heat shock proteins in plants. As the intensity of heat stress increases, it inhibits the energy transfer between light-harvesting complexes and reaction centers, causing the inactivation of the photosynthetic carbon cycle and antioxidant enzyme systems, resulting in reversible or irreversible inactivation of PSII reaction centers, seriously affecting the photochemical reactions of grapes, and then affecting their physiological and biochemical reactions and the expression and regulation of stress-resistant genes. When the stress intensity exceeds its own regulation ability, the plant phenotype will show some heat damage symptoms, and in severe cases, it will lead to plant wilting and even death. Heat stress will seriously hinder the normal photosynthesis and nutrient metabolism of grapes, significantly reducing the yield and quality of grapes, causing many excellent grape varieties to lose their inherent characteristics, and reducing their commerciality and market value (Szenteleki et al., 2012; Asproudiet al., 2016; Wu Jiuyun et al., 2019).

[0003] In order to cope with the impact of heat stress on the grape industry, breeding heat-tolerant grape varieties has become the focus of current research. Heat-tolerant varieties can maintain higher photosynthesis efficiency, cell membrane stability, and antioxidant capacity in high-temperature environments, thus ensuring the yield and quality of fruits. With the continuous increase in the global average temperature, the frequency and duration of high-temperature events are increasing, and the impact on grape production in China will also become increasingly significant. Therefore, it is urgent to find a reasonable and effective method for identifying heat-tolerant grape varieties to identify the heat tolerance of grapes, which is beneficial to exploring heat-tolerant grape germplasm resources and gene resources, laying a foundation for grape genetic improvement and new variety breeding, and is of great significance for cultivating heat-tolerant and high-quality new grape varieties.

[0004] There are many evaluation indicators and methods for plant heat tolerance, including apparent morphology, microscopic structure, physiology and biochemistry, photosynthetic characteristics, etc. At present, the identification of heat-tolerant grape varieties mainly relies on field observation and determination of physiological and biochemical indexes, but these methods have certain problems. The traditional methods for determining physiological and biochemical indexes are cumbersome, time-consuming, lack universal applicability, and are difficult to meet the needs of large-scale screening.

[0005] In summary, how to solve the problems that the traditional methods for measuring physiological and biochemical indexes are cumbersome to operate, time-consuming, and difficult to meet the needs of large-scale screening has become an urgent problem to be solved in this field. Therefore, it is necessary to propose a method for identifying heat-tolerant grape varieties and its application. Summary of the Invention

[0006] To solve the above problems, the present invention provides a method for identifying heat-tolerant grape varieties and its application, which can achieve efficient and accurate identification of heat-tolerant grape varieties by combining high-temperature stress treatment, an automated clamping and detection device, and comprehensive multi-index analysis.

[0007] To achieve the above object, the technical solution of the present invention is as follows: A method for identifying heat-tolerant grape varieties, comprising the following steps:

[0008] Step 1, selection of grape samples: Select different grape fruit and leaf samples; different grape fruit samples include heat-tolerant varieties and common varieties.

[0009] Step 2, high-temperature stress treatment: Set the heat-tolerant variety as the experimental group and the common variety as the control group; place the experimental group and the control group in a high-temperature incubator respectively to obtain the treated experimental group and control group.

[0010] Step 3, determination of physiological and biochemical indexes: Place the treated experimental group and control group on the clamping component of the grape variety determination device respectively to clamp the grape fruit samples until the grape fruit samples are stably clamped, and use the detection component of the grape variety determination device to measure the physiological and biochemical indexes of the grape fruit samples to obtain the physiological and biochemical indexes of the experimental group and the physiological and biochemical indexes of the control group.

[0011] Step 4, result determination: Compare the physiological and biochemical indexes of the experimental group and the physiological and biochemical indexes of the control group to determine the heat tolerance of the heat-tolerant variety.

[0012] The technical principle of the above solution is as follows:

[0013] By placing grape fruits in a high-temperature incubator to simulate the natural high-temperature environment, physiological and biochemical responses of grape fruits are induced. The heat-tolerant varieties are used as the experimental group, and the common varieties are used as the control group. By comparing the changes in physiological and biochemical indexes of the two groups, the tolerance mechanism of heat-tolerant varieties is clarified. High-temperature stress can cause damage to cell membranes, leakage of electrolytes, and an increase in electrical conductivity. The cell membranes of heat-tolerant varieties have higher stability and smaller changes in electrical conductivity. Under high-temperature stress, reactive oxygen species (ROS) accumulate, and the activities of antioxidant enzymes (such as SOD, POD, and CAT) change significantly. Heat-tolerant varieties can maintain higher activities of antioxidant enzymes and reduce oxidative damage. Through the comprehensive determination of electrical conductivity and antioxidant enzyme activities, the heat tolerance of grape fruits is comprehensively evaluated to avoid the limitations of a single index. Through the linkage design of an electric telescopic rod, a piston cylinder, and a clamping plate, stable clamping of grape fruits is achieved, ensuring the fixation and consistency of samples during the detection process. Using the design of a blade and a roller, grape fruits are quickly crushed to facilitate the subsequent determination of physiological and biochemical indexes. Through a physiological and biochemical index detector, the electrical conductivity and antioxidant enzyme activities of grape fruits are automatically measured, improving the detection efficiency and accuracy. By comparing the physiological and biochemical indexes of the experimental group and the control group, the heat-tolerant varieties have smaller changes in electrical conductivity and higher antioxidant enzyme activities. According to the comprehensive results of electrical conductivity and antioxidant enzyme activities, the heat tolerance of grape varieties is determined, and excellent varieties suitable for planting in high-temperature environments are screened out.

[0014] The above scheme has the following beneficial effects:

[0015] 1. Through the automated clamping and detection device, the present invention greatly simplifies the determination process of physiological and biochemical indexes, shortens the detection time, and can meet the needs of large-scale screening of heat-tolerant grape varieties compared with traditional methods, greatly improving the screening efficiency. Compared with the existing technology, this method has the characteristics of being fast, simple, and non-destructive, and can efficiently screen out grape germplasm resources with strong heat resistance, thus laying a foundation for subsequent development and excavation of key heat-resistant gene resources, as well as grape genetic improvement and new variety breeding, which is of great significance for promoting the healthy and sustainable development of the grape industry.

[0016] 2. The present invention adopts comprehensive analysis of multiple indexes, taking into account indexes such as electrical conductivity and antioxidant enzyme activities at the same time, avoiding the limitations of single-index identification, more comprehensively and accurately evaluating the heat tolerance of grape varieties, and improving the reliability of the identification results.

[0017] 3. The identification method and application provided by the present invention help to quickly screen out excellent grape varieties suitable for planting in high-temperature environments, provide support for the grape industry to cope with global warming, ensure the yield and quality of grapes, and promote the sustainable development of the grape industry.

[0018] Further, in step two, the temperature of the high-temperature incubator is set at 42 °C, and the treatment time is set at 24 hours.

[0019] Beneficial effects: The high temperature of 42 °C and the treatment time of 24 hours are parameter settings that have been scientifically verified to effectively simulate the actual high-temperature environment in areas with frequent high temperatures in summer, and are sufficient to induce obvious physiological and biochemical responses in grape fruits, enabling the differences among different grape varieties under high-temperature stress to be fully demonstrated, providing an experimental basis for subsequent index determination and variety identification.

[0020] Further, in step three, the clamping assembly includes a base and a controller. A vertical L-shaped rod is fixedly connected to the top of the base. A clamping seat is fixedly connected to the end of the L-shaped rod away from the base. A through hole is formed in the clamping seat.

[0021] An electric telescopic rod and a piston cylinder are fixedly connected to the top of the horizontal end of the L-shaped rod; the output shaft of the electric telescopic rod is fixedly connected to a first slider in an inverted "V" shape. The bottom of the first slider is slidably matched with the top of the clamping seat. The controller is used to control the telescopic movement of the output shaft of the electric telescopic rod, thereby driving the first slider to slide on the clamping seat; a piston is slidably matched in the piston cylinder. A piston rod is fixedly connected to the piston. The end of the piston rod away from the piston is fixedly connected to the side wall of the first slider.

[0022] Second sliders are symmetrically and slidably matched with the top of the clamping seat. The first slider is slidably matched with the adjacent second slider; a connecting rod is fixedly connected to one side wall of each second slider. The end of the connecting rod away from the second slider is fixedly connected to a clamping plate.

[0023] A fixing block is also fixedly connected to the top of the clamping seat. A connecting rod is also fixedly connected to one side wall of the fixing block. The end of the connecting rod away from the fixing block is also fixedly connected to a clamping plate.

[0024] Air holes are formed in the side walls of the clamping plates away from the connecting rods. The piston cylinder and the air holes are all connected. One-way valves are connected to the air holes.

[0025] A crushing assembly for crushing grape fruits and a driving assembly for driving the crushing assembly to tilt are provided on the L-shaped rod.

[0026] Beneficial effects: The clamping assembly can ensure the fixation of grape fruits during the detection process, avoiding affecting the detection results due to shaking or displacement; on the other hand, the symmetrically arranged clamping plates and the generation of negative pressure in the air holes through the connection between the piston cylinder and the air holes can attract grape fruits, and can adapt to grape fruits of different sizes and shapes.

[0027] Further, the crushing assembly includes a crushing box and a cylinder. The cylinder is fixedly connected to the bottom of the clamping seat, and the output shaft of the cylinder is hinged to the top of the crushing box. An opening is formed at the top of the crushing box, and the opening is communicated with the through hole. One side wall of the crushing box is hinged to the side wall of the L-shaped rod, and a discharge pipe is communicated with the side wall of the crushing box away from the L-shaped rod. A driving member is fixedly connected to the inner side wall of the crushing box, a roller shaft is coaxially fixedly connected to the output shaft of the driving member, a plurality of blades are fixedly connected to the roller shaft, and the controller is used to control the rotation of the output shaft of the driving member to drive the blades to rotate.

[0028] Beneficial effects: The crushing assembly can quickly crush the grape fruits after being stably clamped, which is convenient for subsequent determination of physiological and biochemical indexes. By controlling the driving member through the controller, the blades on the roller shaft rotate at a high speed, efficiently crushing the grape fruits and improving the sample processing efficiency. The setting of the cylinder can tilt the crushing box, facilitating the discharge of the crushed grape pulp through the discharge pipe, smoothly connecting with the detection assembly, and ensuring the coherence and high efficiency of the entire detection process.

[0029] Further, the driving assembly includes an air storage tank, and the air storage tank is fixedly connected to the side wall of the L-shaped rod away from the crushing box; the air storage tank is communicated with the piston cylinder, a trachea is fixedly connected between the air storage tank and the input end of the cylinder, a first solenoid valve is communicated in the trachea, and the controller is used to control the opening and closing of the first solenoid valve.

[0030] A reset assembly is provided at the bottom of the clamping seat for resetting the tilted crushing box.

[0031] Beneficial effects: The driving assembly, through the cooperation of the air storage tank, the trachea and the first solenoid valve, utilizes the gas pressure generated by the piston cylinder during the clamping process to provide power for the cylinder, realizing the automatic tilting of the crushing box, achieving the recycling of power, and saving additional energy consumption. The reset assembly ensures that the crushing box can be reset after completing the crushing and discharging operations, preparing for the next crushing operation, and improving the working efficiency and stability of the equipment.

[0032] Further, the detection assembly includes a physiological and biochemical index detector, and the physiological and biochemical index detector is fixedly connected to the top of the base, and the input end of the physiological and biochemical index detector is communicated with the discharge pipe. A support rod is also fixedly connected to the top of the clamping seat, a camera is fixedly connected to the top of the support rod, and the controller is used to control the camera to take images of the grape fruit samples.

[0033] A flushing assembly is also provided on the L-shaped rod for flushing the inside of the crushing box.

[0034] Beneficial effects: The flushing assembly can flush the crushing box after each detection, avoiding the interference of residual pulp on the next detection result, and ensuring the cleanliness of the detection equipment and the reliability of the detection result.

[0035] Further, the flushing assembly includes a water tank fixedly connected to one side wall of the L-shaped rod; a flushing pipe is fixedly connected and communicated between the water tank and the crushing box, and a second solenoid valve is communicated inside the flushing pipe. The controller is used to control the opening and closing of the second solenoid valve.

[0036] Beneficial effects: By controlling the opening and closing of the second solenoid valve through the controller, the flushing timing and flushing water volume of the water tank for the crushing box can be accurately controlled.

[0037] Further, the reset assembly includes an electromagnet and an iron block. The electromagnet is fixedly connected to the bottom of the clamping seat, and the iron block is fixedly connected to the top of the crushing box. The iron block and the electromagnet correspond to each other, and the controller is used to control the opening and closing of the electromagnet.

[0038] Beneficial effects: Utilizing the magnetic attraction principle of the electromagnet and the iron block, under the control of the controller, when the electromagnet is energized, it generates a magnetic force to attract the iron block, thereby realizing the rapid reset of the crushing box.

[0039] Further, in step three, the physiological and biochemical indexes include conductivity and antioxidant enzyme activity.

[0040] Beneficial effects: Conductivity can intuitively reflect the damage degree of the cell membrane of grape fruits under high-temperature stress, and the antioxidant enzyme activity reflects the ability of grape fruits to cope with oxidative damage.

[0041] Further, the application of a method for identifying heat-tolerant grape varieties includes the following application scenarios: applied to grape breeding, cultivation and promotion, and scientific research.

[0042] Beneficial effects: The method for identifying heat-tolerant grape varieties is applied to grape breeding. By screening heat-tolerant varieties, the breeding plan is optimized; in cultivation and promotion, this method is used to screen grape varieties suitable for planting in high-temperature areas; in scientific research, it is used to analyze the physiological and molecular mechanisms of grape heat tolerance.

[0043] The additional aspects and advantages of the present invention will be partially given in the following description, partially become obvious from the following description, or be understood through the practice of the present invention. Description of the Drawings

[0044] Figure 1 It is a step diagram of the method for identifying heat-tolerant grape varieties of the present invention.

[0045] Figure 2 It is an axonometric view of the grape variety measuring device in the method for identifying heat-tolerant grape varieties of the present invention.

[0046] Figure 3 It is a top view of the grape variety measuring device in the method for identifying heat-tolerant grape varieties of the present invention.

[0047] Figure 4It is a side sectional view of the crushing box in the grape variety measuring device in the identification method of the heat-resistant grape variety of the present invention.

[0048] Figure 5 It is a side sectional view of the piston cylinder in the grape variety measuring device in the identification method of the heat-resistant grape variety of the present invention.

[0049] The reference numerals in the accompanying drawings of the specification include: 1, base; 2, L-shaped rod; 3, clamping seat; 4, electric telescopic rod; 5, piston cylinder; 6, first slider; 7, piston; 8, piston rod; 9, second slider; 10, connecting rod; 11, clamping plate; 12, fixed block; 13, camera; 14, crushing box; 15, cylinder; 16, driving member; 17, roller shaft; 18, blade; 19, discharge pipe; 20, air storage tank; 21, support rod; 22, electromagnet; 23, iron block; 24, physiological and biochemical index detector. Detailed implementation manners

[0050] The following is a further detailed description through specific implementation manners:

[0051] Example 1:

[0052] As shown in the attachment Figure 1 A method for identifying heat-resistant grape varieties includes the following steps:

[0053] Step 1, selection of grape samples: Select different grape fruit samples; the different grape fruit samples include heat-resistant varieties and common varieties.

[0054] Step 2, high-temperature stress treatment: Set the heat-resistant variety as the experimental group and the common variety as the control group; place the experimental group and the control group in a high-temperature incubator respectively to obtain the treated experimental group and control group.

[0055] In Step 2, the temperature of the high-temperature incubator is set to 42 °C and the treatment time is set to 24 hours.

[0056] Step 3, determination of physiological and biochemical indexes: Place the treated experimental group and control group on the clamping assembly of the grape variety measuring device respectively to clamp the grape fruit samples until the grape fruit samples are stably clamped, and use the detection assembly of the grape variety measuring device to measure the physiological and biochemical indexes of the grape fruit samples to obtain the physiological and biochemical indexes of the experimental group and the physiological and biochemical indexes of the control group.

[0057] Step 4, result determination: Compare the physiological and biochemical indexes of the experimental group and the physiological and biochemical indexes of the control group to determine the heat resistance of the heat-resistant variety.

[0058] Among them, in Step 3, the physiological and biochemical indexes include conductivity and antioxidant enzyme activity.

[0059] Application of the identification method for heat-tolerant grape varieties, including the following application scenarios: applied to grape breeding, cultivation promotion, and scientific research.

[0060] The specific implementation process is as follows:

[0061] Select fruit samples from different grape varieties, ensuring that the samples include known heat-tolerant varieties and common varieties. Select 10 samples for each variety to ensure the statistical significance of the experiment.

[0062] Divide the selected grape fruit samples into an experimental group and a control group; the experimental group is the heat-tolerant variety, and the control group is the common variety.

[0063] Put the grape fruits in the experimental group and the control group into a high-temperature incubator respectively, set the temperature to 42 °C, and the treatment time to 24 hours. The setting of this temperature and time can effectively simulate the high-temperature environment in summer and induce obvious physiological and biochemical responses in grape fruits.

[0064] Put the treated experimental group and control group on the clamping component of the grape variety measuring device to clamp the grape fruits until the grape fruits are clamped stably, and use the detection component of the grape variety measuring device to measure the physiological and biochemical indexes of the grapes to obtain the physiological and biochemical indexes of the experimental group and the control group.

[0065] Compare and analyze the physiological and biochemical indexes of the experimental group and the control group. The change in conductivity of the heat-tolerant variety is small, indicating that its cell membrane stability is high; the antioxidant enzyme activity is high, indicating that its antioxidant ability is strong.

[0066] Judge the heat tolerance of grape varieties according to the comprehensive results of conductivity and antioxidant enzyme activity. Screen out excellent varieties suitable for planting in high-temperature environments.

[0067] Through this method, screen heat-tolerant varieties, optimize the breeding plan, and cultivate new grape varieties more suitable for planting in high-temperature environments; promote heat-tolerant grape varieties in areas with frequent high temperatures to ensure the yield and quality of grapes and improve economic benefits; use this method to analyze the physiological and molecular mechanisms of grape heat tolerance and provide theoretical support for grape heat-tolerant breeding.

[0068] Through the above steps, the present invention provides an efficient and accurate identification method for heat-tolerant grape varieties, which can meet the needs of large-scale screening, provides technical support for the grape industry to cope with global warming, and promotes the sustainable development of the grape industry.

[0069] Example 2:

[0070] As attached Figures 2 - 5As shown, the difference from Embodiment 1 is that the clamping assembly includes a base 1 and a controller. At the top of the base 1, an L-shaped rod 2 is fixedly connected by screws. At one end of the L-shaped rod 2 away from the base 1, a clamping seat 3 is integrally formed. A through hole is formed in the clamping seat 3.

[0071] At the top of the horizontal end of the L-shaped rod 2, an electric telescopic rod 4 and a piston cylinder 5 are fixedly connected by screws; the output shaft of the electric telescopic rod 4 is fixedly connected by screws with a first slider 6 in the shape of "︹". The bottom of the first slider 6 and the top of the clamping seat 3 are slidably matched. The controller is used to control the telescopic movement of the output shaft of the electric telescopic rod 4, thereby driving the first slider 6 to slide on the clamping seat 3; a piston 7 is slidably matched in the piston cylinder 5. A piston rod 8 is integrally formed on the piston 7. One end of the piston rod 8 away from the piston 7 is fixedly connected to the side wall of the first slider 6 by screws.

[0072] On the top of the clamping seat 3, second sliders 9 are symmetrically and slidably matched. The first slider 6 is slidably matched with the adjacent second slider 9; on one side wall of each second slider 9, a connecting rod 10 is fixedly connected by screws. One end of the connecting rod 10 away from the second slider 9 is fixedly connected to a clamping plate 11 by screws.

[0073] A fixed block 12 is fixedly connected to the top of the clamping seat 3 by screws. A connecting rod 10 is also fixedly connected to one side wall of the fixed block 12 by screws. One end of the connecting rod 10 away from the fixed block 12 is also fixedly connected to a clamping plate 11 by screws.

[0074] Air holes are formed on the side wall of the clamping plate 11 away from the connecting rod 10. The piston cylinder 5 and the air holes are all communicated. One-way valves are communicated in the air holes.

[0075] A crushing assembly for crushing grape fruits and a driving assembly for driving the crushing assembly to tilt are provided on the L-shaped rod 2.

[0076] The crushing assembly includes a crushing box 14 and a cylinder 15. The cylinder 15 is fixedly connected to the bottom of the clamping seat 3 by screws. The output shaft of the cylinder 15 and the top of the crushing box 14 are hinged; an opening is formed at the top of the crushing box 14, and the opening is communicated with the through hole; one side wall of the crushing box 14 and the side wall of the L-shaped rod 2 are hinged. A discharge pipe 19 is communicated with the side wall of the crushing box 14 away from the L-shaped rod 2; a driving member 16 is fixedly connected to the inner side wall of the crushing box 14 by screws. A roller shaft 17 is coaxially integrally formed on the output shaft of the driving member 16. A plurality of blades 18 are welded on the roller shaft 17. The controller is used to control the rotation of the output shaft of the driving member 16 to drive the blades 18 to rotate.

[0077] The driving assembly includes an air storage tank 20. The air storage tank 20 is fixedly connected to the side wall of the L-shaped rod 2 away from the crushing box 14 by screws; the air storage tank 20 is communicated with the piston cylinder 5. A trachea is fixedly communicated between the air storage tank 20 and the input end of the cylinder 15. A first electromagnetic valve is communicated in the trachea. The controller is used to control the opening and closing of the first electromagnetic valve.

[0078] A reset component for resetting the tilted crushing box 14 is provided at the bottom of the clamping seat 3.

[0079] The detection component includes a physiological and biochemical index detector 24, which is fixedly connected to the top of the base 1 by screws. The input end of the physiological and biochemical index detector 24 is communicated with the discharge pipe 19. A support rod 21 is also fixedly connected to the top of the clamping seat 3 by screws, and a camera 13 is fixedly connected to the top of the support rod 21 by screws. The controller is used to control the camera 13 to take images of grape fruit samples.

[0080] A flushing component for flushing the inside of the crushing box 14 is also provided on the L-shaped rod 2.

[0081] The flushing component includes a water tank, which is fixedly connected to one side wall of the L-shaped rod 2 by screws; a flushing pipe is fixedly connected between the water tank and the crushing box 14, and a second solenoid valve is connected in the flushing pipe. The controller is used to control the opening and closing of the second solenoid valve.

[0082] The reset component includes an electromagnet 22 and an iron block 23. The electromagnet 22 is fixedly connected to the bottom of the clamping seat 3 by screws, and the iron block 23 is fixedly connected to the top of the crushing box 14 by screws. The iron block 23 corresponds to the electromagnet 22, and the controller is used to control the opening and closing of the electromagnet 22.

[0083] The specific implementation process is as follows:

[0084] Select grape fruit and leaf samples from different grape varieties to ensure that the samples include known heat-resistant varieties and common varieties. Select 10 samples for each variety to ensure the statistical significance of the experiment.

[0085] Divide the selected grape fruit and leaf samples into an experimental group and a control group; the experimental group is a heat-resistant variety, and the control group is a common variety.

[0086] Put the grape fruit samples and leaf samples of the experimental group and the control group into a high-temperature incubator respectively, set the temperature to 42 °C, and the treatment time to 24 hours.

[0087] In Figure 2 , Figure 3 and Figure 5For example, place the processed grape fruit sample of the experimental group above the through hole of the clamping seat 3 between the clamping plates 11. Start the electric telescopic rod 4 through the controller. The output shaft of the electric telescopic rod 4 extends, driving the first slider 6 to slide to the right on the top of the clamping seat 3. Since the first slider 6 is in the shape of "︹", at this time, the first slider 6 can drive the adjacent second slider 9 to slide synchronously towards the direction close to the grape fruit sample, and then drive all the connecting rods 10 to drive all the clamping plates 11 to approach the grape fruit sample, realizing the clamping of the grape fruit sample. At the same time, the rightward movement of the first slider 6 can pull the piston 7 in the piston cylinder 5 to move to the right, generating negative pressure in the piston cylinder 5. Since the air hole and the piston cylinder 5 are both connected, at this time, negative pressure is generated in the air hole, attracting the grape fruit sample, ensuring that the grape fruit sample is further stably clamped. At the same time, due to the effect of negative pressure, for irregularly shaped grape fruit samples, stable clamping can also be achieved.

[0088] After the grape fruit sample is stably clamped, the controller controls the camera 13 to take pictures of the grape fruit sample in the middle of the clamping seat 3 to obtain the image shape and color information of the grape fruit sample.

[0089] Take Figure 2 and Figure 4 For example, at the same time, the controller controls the output shaft of the driving member 16 to rotate. In this embodiment, the driving member 16 is a DC motor; the output shaft of the DC motor drives the roller shaft 17 and the blade 18 on the roller shaft 17 to rotate at high speed; at the same time, the controller controls the output shaft of the electric telescopic rod 4 to contract. At this time, the output shaft of the electric telescopic rod 4 drives the first slider 6 to slide to the left, and then drives all the second sliders 9 to slide away from the grape fruit sample, and then the clamping plate 11 releases the grape fruit sample. At this time, due to the action of gravity, the grape fruit sample falls into the inside of the crushing box 14 from the opening at the top of the crushing box 14, and the high-speed rotating blade 18 crushes the grape fruit sample.

[0090] Take Figure 2 and Figure 5 For example, while the first slider 6 moves to the left, it can drive the piston 7 to move to the left. Since the air storage tank 20 and the piston cylinder 5 are connected, and at the same time due to the action of the one-way valve in the air hole, the gas cannot flow out of the air hole. At this time, the piston 7 presses the gas in the piston cylinder 5 into the air storage tank 20 for storage. At the same time, the controller controls the first electromagnetic valve to open. Since the air storage tank 20 is also connected to the cylinder 15, at this time, the gas in the air storage tank 20 can enter the cylinder 15 from the input end of the cylinder 15. At this time, the output shaft of the cylinder 15 extends. Since the crushing box 14 is hinged to the side wall of the L-shaped rod 2, at this time, the crushing box 14 tilts to the right in the shape of "\", and the crushed grape juice inside the crushing box 14 flows into the physiological and biochemical index detector 24 from the discharge pipe 19 under the action of gravity, for the determination of the conductivity and antioxidant enzyme activity of the grape juice, and the physiological and biochemical indexes of the experimental group are obtained.

[0091] Subsequently, the controller opens the second solenoid valve in the flushing pipe, and the water in the water tank flows into the crushing box 14 from the flushing pipe to clean the crushing box 14, avoiding interference from residual pulp with the next detection.

[0092] Take Figure 2 as an example. After the measurement is completed, the controller activates the electromagnet 22. The electromagnet 22 is energized to generate a magnetic force to attract the iron block 23, causing the crushing box 14 to reset upward. At this time, the upward reset of the crushing box 14 can drive the output shaft of the cylinder 15 to contract, causing the gas in the cylinder 15 to return to the air storage tank 20 for storage, so as to facilitate the continuation of the next measurement.

[0093] According to the same operation process, the grape fruit samples in the control group are clamped, crushed, detected and cleaned to obtain the physiological and biochemical indexes of the control group.

[0094] Compare and analyze the physiological and biochemical indexes of the experimental group and the control group. The change in conductivity of the heat-resistant variety is small, indicating that its cell membrane stability is high; the antioxidant enzyme activity is high, indicating that its antioxidant ability is strong.

[0095] Based on the comprehensive results of conductivity and antioxidant enzyme activity, determine the heat resistance of grape varieties. Screen out excellent varieties suitable for planting in high-temperature environments.

[0096] By screening heat-resistant varieties with this method, optimizing the breeding plan, and cultivating new grape varieties more suitable for planting in high-temperature environments; promoting heat-resistant grape varieties in areas with frequent high temperatures to ensure the yield and quality of grapes and improve economic benefits; using this method to analyze the physiological and molecular mechanisms of grape heat resistance and provide theoretical support for grape heat-resistant breeding.

[0097] Obviously, the above embodiments are merely examples given for clear illustration and are not limitations on the implementation manners. For those of ordinary skill in the art, other different forms of changes or modifications can be made based on the above description. It is not necessary and impossible to list all the implementation manners here. And the obvious changes or modifications derived therefrom still fall within the protection scope of the present invention.

Claims

1. A method for identifying high temperature resistant grape varieties, characterized in that: The following steps are involved: Step 1: grape sample selection: select different grape fruit and leaf samples; different grape fruit samples include high temperature resistant varieties and common varieties; Step 2: high temperature stress treatment: setting the high temperature resistant varieties as the experimental group and the common varieties as the control group; placing the experimental group and the control group in a high temperature incubator for treatment, respectively, to obtain the treated experimental group and the control group; Step 3, physiological and biochemical index determination: the treated experimental group and control group are respectively placed on the clamping components of the grape variety determination device to clamp the grape fruit samples until the grape fruit samples are clamped stably, and the detection component of the grape variety determination device is used to determine the physiological and biochemical indexes of the grape fruit samples to obtain the physiological and biochemical indexes of the experimental group and the physiological and biochemical indexes of the control group; Step 4, result determination: Compare the physiological and biochemical indicators of the experimental group with those of the control group to determine the high temperature resistance of the high temperature resistant varieties.

2. The method for identifying high temperature resistant grape varieties according to claim 1, characterized in that: In step 2, the temperature of the high temperature incubator is set to 42°C and the processing time is set to 24 hours.

3. The method for identifying high temperature resistant grape varieties according to claim 2, characterized in that: In step 3, the clamping assembly includes a base (1) and a controller; An L-shaped rod (2) is fixedly connected to the top of the base (1), and a clamping seat (3) is fixedly connected to one end of the L-shaped rod (2) away from the base (1), and a through hole is formed on the clamping seat (3); The top of the horizontal end of the L-shaped rod (2) is fixedly connected to an electric telescopic rod (4) and a piston cylinder (5); the output shaft of the electric telescopic rod (4) is fixedly connected to a first slider (6) in the shape of "︹", the bottom of the first slider (6) and the top of the clamping seat (3) are slidably matched, and the controller is used to control the extension and retraction of the output shaft of the electric telescopic rod (4), thereby driving the first slider (6) to slide on the clamping seat (3); a piston (7) is slidably matched in the piston cylinder (5), and a piston rod (8) is fixedly connected to the piston (7), and the end of the piston rod (8) away from the piston (7) is fixedly connected to the side wall of the first slider (6); The top of the clamping seat (3) is symmetrically and slidably matched with a second slider (9), and the first slider (6) is slidably matched with the second slider (9) adjacent thereto; a connecting rod (10) is fixedly connected to one side wall of the second slider (9), and a clamping plate (11) is fixedly connected to one end of the connecting rod (10) away from the second slider (9); A fixing block (12) is fixedly connected to the top of the clamping seat (3), a connecting rod (10) is also fixedly connected to a side wall of the fixing block (12), and a clamping plate (11) is also fixedly connected to one end of the connecting rod (10) away from the fixing block (12); A pore is formed on a wall of the clamping plate (11) away from the connecting rod (10), the piston cylinder (5) and the pore are connected, and a one-way valve is connected in the pore; The L-shaped rod (2) is provided with a crushing assembly for crushing grape fruits and a driving assembly for driving the crushing assembly to tilt.

4. The method for identifying high temperature resistant grape varieties according to claim 3, characterized in that: The crushing assembly comprises a crushing box (14) and a cylinder (15); The cylinder (15) is fixedly connected to the bottom of the clamping seat (3), and the output shaft of the cylinder (15) is hinged to the top of the crushing box (14); the top of the crushing box (14) is provided with an opening, and the opening is communicated with the through hole; one side wall of the crushing box (14) is hinged to the side wall of the L-shaped rod (2), and a discharge pipe (19) is communicated on the side wall of the crushing box (14) away from the L-shaped rod (2); a driving member (16) is fixedly connected to the inner side wall of the crushing box (14), a roller shaft (17) is coaxially fixedly connected to the output shaft of the driving member (16), and a plurality of blades (18) are fixedly connected to the roller shaft (17), and a controller is used to control the output shaft of the driving member (16) to rotate, so as to drive the blades (18) to rotate.

5. The method for identifying high temperature resistant grape varieties according to claim 4, characterized in that: The driving assembly comprises an air storage box (20), the air storage box (20) being fixedly connected to a side wall of the L-shaped rod (2) away from the crushing box (14); the air storage box (20) is connected to the piston cylinder (5), an air pipe is fixedly connected between the air storage box (20) and the input end of the cylinder (15), a first electromagnetic valve is connected in the air pipe, and a controller is used to control the opening and closing of the first electromagnetic valve; A reset component for resetting the tilted crushing box (14) is provided at the bottom of the clamping seat (3).

6. The method for identifying high temperature resistant grape varieties according to claim 5, characterized in that: The detection component comprises a physiological and biochemical index detector (24), which is fixedly connected to the top of the base (1), and the input end of the physiological and biochemical index detector (24) is connected to the discharge pipe (19); the top of the clamping seat (3) is also fixedly connected to a support rod (21), and the top of the support rod (21) is fixedly connected to a camera (13), and the controller is used to control the camera (13) to capture an image of the grape fruit sample; The L-shaped rod (2) is also provided with a flushing assembly for cleaning the inside of the crushing box (14).

7. The method for identifying high temperature resistant grape varieties according to claim 6, characterized in that: The flushing assembly comprises a water tank, which is fixedly connected to a side wall of the L-shaped rod (2); a flushing pipe is fixedly connected between the water tank and the crushing box (14), a second solenoid valve is connected in the flushing pipe, and a controller is used to control the opening and closing of the second solenoid valve.

8. The method for identifying high temperature resistant grape varieties according to claim 7, characterized in that: The reset assembly comprises an electromagnet (22) and an iron block (23), wherein the electromagnet (22) is fixedly connected to the bottom of the clamping seat (3), and the iron block (23) is fixedly connected to the top of the crushing box (14), the iron block (23) corresponds to the electromagnet (22), and the controller is used to control the opening and closing of the electromagnet (22).

9. The method for identifying high temperature resistant grape varieties according to claim 8, characterized in that: In step three, the physiological and biochemical indicators include electrical conductivity and antioxidant enzyme activity.

10. An application of a method for identifying a high temperature resistant grape variety, based on the method for identifying a high temperature resistant grape variety according to any one of claims 1 to 9, characterized in that: Including the following application scenarios: applied in grape breeding, cultivation promotion and scientific research.

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