Immunochromatography multichannel sample quantitative detection device

Abstract

The invention relates to the technical field of immunochromatography analysis, in particular to an immunochromatography multichannel sample quantitative detection device which comprises an upper shell and a lower shell. The upper shell is provided with an observation window and a sample adding hole; the sample adding hole is embedded into the upper shell, and at least two through holes are uniformly formed in the lower surface of the sample adding hole at intervals; a flow guide column extending towards the direction of the lower shell is arranged on the lower surface of the sample adding hole; uniformly distributed grids which at least cover all the through holes are also arranged in the sample adding hole; step structures are arranged in spaces between the uniformly distributed grids and the lower surfaces of the sample adding holes and are distributed among through holes formed in the lower surfaces of the sample adding holes; a test paper groove and a test paper support are arranged on the upper surface of the lower shell; a group of buckle structures are arranged at opposite positions of the lower surface of the upper shell and the upper surface of the lower shell and used for buckling the upper shell and the lower shell, a drainage angle is optionally arranged between each flow guide column and the adjacent through hole, and the step structure is a prismatic table structure with 2-5 steps on two sides in the extending direction from the upper shell to the lower shell.

Description

Technical Field

[0001] This application relates to the technical field of immunochromatographic analysis, and more particularly, to an immunochromatographic multi-channel sample quantitative detection device. Background Art

[0002] Colloidal gold is negatively charged in a weak alkaline environment and can form a firm bond with the positively charged groups of protein molecules. Since this bond is an electrostatic bond, it does not affect the biological properties of proteins. In addition to binding to proteins, colloidal gold can also bind to many other biological macromolecules, such as SPA, PHA, ConA, etc. According to some physical properties of colloidal gold, such as high electron density, particle size, shape, and color reaction, combined with the immunological and biological properties of the conjugate, colloidal gold is thus widely used in the fields of immunology, histology, pathology, and cell biology. Colloidal gold labeling is essentially a coating process in which macromolecules such as proteins are adsorbed onto the surface of colloidal gold particles. The adsorption mechanism may be that the negatively charged surface of colloidal gold particles forms a firm bond with the positively charged groups of proteins due to electrostatic adsorption. The immunogold labeling technique mainly utilizes the property of gold particles having high electron density. At the binding site of the gold-labeled protein, dark brown particles can be seen under a microscope. When these markers accumulate in large numbers at the corresponding ligand, a red or pink spot can be seen with the naked eye. Therefore, it is used in qualitative or semi-quantitative rapid immunoassay methods. This reaction can also be amplified by the deposition of silver particles, which is called immunogold-silver staining. The colloidal gold technique has the advantages of being convenient and fast, specific and sensitive, stable, not requiring special equipment and reagents, and having an intuitive result judgment. Therefore, it is especially suitable for grass-roots inspection personnel, large-scale detection, and large-area general surveys, and has great development potential and broad application prospects.

[0003] The principle of immunochromatography is to first fix a specific antibody on a certain zone of a nitrocellulose membrane. When one end of the dried nitrocellulose is immersed in a sample (urine or serum), due to capillary action, the sample will move forward along the membrane. When it moves to the area where the antibody is fixed, the corresponding antigen in the sample will specifically bind to the antibody. If immunocolloidal gold or immunoenzyme staining is used, a certain color can be shown in this area, thus achieving specific immunodiagnosis.

[0004] Currently, in multi - card immunoassay test cards, multiple sample - adding holes correspond to multiple test strips integrated on the same card box. Each test strip corresponds to a different test item. During testing, the sample needs to be added to the sample - adding ports to be tested one by one, which increases the number of operations and operation time, and is prone to operation errors. It is very easy to miss adding the sample or add the sample repeatedly. In the case of precious samples with small amounts, it cannot meet the needs of multiple detections at the same time, and precious samples are easily lost. Therefore, there is an urgent need to develop an immunochromatographic and immunofluorescent test card box with a simple structure, easy to use, and capable of accurately measuring multiple items at one time. Summary of the Invention

[0005] In order to solve the problem in the prior art that for different test items, the sample needs to be added to the sample - adding ports to be tested one by one during testing, which increases the number of operations and operation time, and is prone to operation errors, this application provides an immunochromatographic multi - channel sample quantitative detection device including a structure for evenly distributing quantitative liquid, which consists of a uniformly distributed grid, a stepped structure, a diversion column, and a drainage angle.

[0006] The technical solution of this application is as follows:

[0007] This application provides an immunochromatographic multi - channel sample quantitative detection device, which includes: an upper housing; and a lower housing; an observation window and a sample - adding hole are provided on the upper surface of the upper housing; the sample - adding hole is embedded in the upper housing, and at least two through - holes are evenly spaced on the lower surface of the sample - adding hole; a diversion column extending towards the lower housing is provided on the lower surface of the sample - adding hole; a uniformly distributed grid covering all the through - holes is further provided in the sample - adding hole; a stepped structure is arranged in the space between the uniformly distributed grid and the lower surface of the sample - adding hole, and is distributed between the through - holes provided on the lower surface of the sample - adding hole; a test strip groove and a test strip holder are provided on the upper surface of the lower housing; a set of snap - fit structures are provided at the relative positions of the lower surface of the upper housing and the upper surface of the lower housing for snapping the upper housing and the lower housing together.

[0008] Further, the diversion column abuts against the through - hole.

[0009] Further, the distance between the diversion column and the through - hole is 0 - 0.5 mm, and an optional drainage angle is provided between each diversion column and the adjacent through - hole.

[0010] Further, the angle of the drainage angle facing the through - hole forms an angle of 30 - 60° with the lower surface of the sample - adding hole.

[0011] Further, the number of through - holes is odd, preferably the aperture of the middle through - hole is larger than that of the other through - holes, and further preferably, no diversion column is provided around the middle through - hole.

[0012] Further, the number of the through holes is an even number. Preferably, the apertures of the through holes are uniform. More preferably, a flow guiding post is arranged around each through hole.

[0013] Further, the aperture of the middle through hole is 2.0 - 4.0 mm; the apertures of the remaining through holes are 1.5 - 3.5 mm.

[0014] Further, the apertures of the through holes are all 1.5 - 3.5 mm.

[0015] Further, in the direction extending from the upper housing towards the lower housing, the stepped structure is a frustum structure with 2 - 5 levels of steps on both sides.

[0016] Further, a stepped structure is arranged between every two adjacent through holes.

[0017] Further, the height of each level of step is 0.1 - 2 mm, and the maximum width of the stepped structure is 1 - 3 mm.

[0018] Further, the number of the observation windows is the same as the number of the through holes, and they are arranged in an array or side by side. The number of the through holes is the same as the number of the test strips.

[0019] Further, a support groove is arranged around the observation window on the lower surface of the upper housing.

[0020] Further, a plurality of support bars are also arranged on the lower surface of the upper housing.

[0021] Further, the periphery of the test strip groove can form a fixing groove surrounding at most three sides to fix the test strip.

[0022] The beneficial effects of the present application are as follows:

[0023] In the present application, four structures for uniformly distributing quantitative liquid, namely a uniformly distributed grid, a stepped structure, a flow guiding post, and a drainage angle, are arranged in the immunochromatographic analysis multi-channel sample quantitative detection device to assist in quantitative average shunting. The uniformly distributed grid can make the added test solution spread out on the grid first, avoiding the concentrated flow of the test solution; according to different quantification requirements, the stepped structure is provided with different numbers of steps and different step heights to assist in quantitative shunting; both the flow guiding post and the drainage angle have the function of guiding the test solution to flow into the test paper to different extents; through the above four structures for uniformly distributing quantitative liquid, quantitative average shunting is assisted. BRIEF DESCRIPTION OF THE DRAWINGS

[0024] The drawings are used to better understand the present application and do not constitute an improper limitation to the present application. Among them:

[0025] Figure 1Stereogram of the immunochromatographic multi-channel sample quantitative detection device provided by the present application;

[0026] Figure 2 Structural schematic diagram of the immunochromatographic multi-channel sample quantitative detection device provided by the present application;

[0027] Figure 3 Cross-sectional view of the immunochromatographic multi-channel sample quantitative detection device provided by the present application;

[0028] Figure 4 Schematic diagram of the lower surface of the upper housing of the immunochromatographic multi-channel sample quantitative detection device provided by the present application;

[0029] Figure 5 Partial schematic diagram of the sample adding hole of the immunochromatographic multi-channel sample quantitative detection device provided by the present application.

[0030] Explanation of reference numerals:

[0031] 1 - upper housing, 2 - lower housing, 101 - observation window, 102 - sample adding hole, 103 - support groove, 104 - buckle structure, 105 - stepped structure, 106 - diversion column, 107 - drainage angle, 108 - uniformly distributed grid, 109 - support bar, 201 - test strip slot, 202 - fixing groove, 203 - test strip holder, 204 - buckle structure, 1021 - first through hole, 1022 - second through hole, 1023 - third through hole. Detailed implementation manners

[0032] The specific embodiments of the present application will be described in more detail with reference to the accompanying drawings. Although the specific embodiments of the present application are shown in the drawings, it should be understood that the present application can be implemented in various forms and should not be limited by the embodiments described herein. On the contrary, these embodiments are provided to enable a more thorough understanding of the present application and to be able to fully convey the scope of the present application to those skilled in the art.

[0033] It should be noted that certain terms are used in the specification and claims to refer to specific components. Those skilled in the art should understand that technicians may use different terms to refer to the same component. The specification and claims do not use the difference in terms as a way to distinguish components, but use the difference in the functions of components as the criterion for distinction. Such as the term "comprising" or "including" mentioned throughout the specification and claims is an open-ended term, so it should be interpreted as "including but not limited to". The subsequent description in the specification is the preferred implementation manner for implementing the present application, but the description is for the purpose of the general principle of the specification and is not used to limit the scope of the present application. The protection scope of the present application shall be determined by what is defined by the appended claims.

[0034] For the convenience of understanding the specific solution of the present application, the following will further explain with several specific embodiments in conjunction with the attached drawings, and each attached drawing does not constitute a limitation to the present application.

[0035] In the description of the present application, it should be understood that for the orientation description, such as the orientation or positional relationship indicated by up, down, front, back, left, right, etc. is based on the orientation or positional relationship shown in the attached drawings, and it is only for the convenience of describing the present application and simplifying the description, rather than indicating or implying that the device or element referred to must have a specific orientation, be constructed and operated in a specific orientation, so it cannot be understood as a limitation to the present application.

[0036] In the description of the present application, unless otherwise clearly defined, words such as setting, installing, connecting, etc. should be understood in a broad sense, and those skilled in the art can reasonably determine the specific meaning of the above words in the present application in combination with the specific content of the technical solution.

[0037] In the description of the present application, "abutment" means that two objects are vertically connected.

[0038] The present application provides an immunochromatographic multi-channel sample quantitative detection device, which includes: an upper housing 1; and a lower housing 2; an observation window 101 and a sample adding hole 102 are provided on the upper surface of the upper housing 1; the sample adding hole 102 is embedded in the upper housing 1, and at least two through holes are evenly spaced on the lower surface of the sample adding hole 102; a diversion column 106 extending towards the lower housing is provided on the lower surface of the sample adding hole 102; a uniform grid 108 for at least covering all the through holes is further provided in the sample adding hole 102; a stepped structure 105 is provided in the space between the uniform grid 108 and the lower surface of the sample adding hole 102, and is distributed between the through holes provided on the lower surface of the sample adding hole 102; a test strip slot 201 and a test strip holder 203 are provided on the upper surface of the lower housing 2; a set of snap structures are provided at the relative positions of the lower surface of the upper housing 1 and the upper surface of the lower housing 2 for snapping the upper housing 1 and the lower housing 2 together.

[0039] In a specific embodiment of the present application, there is no specific limitation on the materials of the upper housing 1 and the lower housing 2, and any material that those skilled in the art consider suitable for the device housing can be used, such as acrylonitrile-butadiene-styrene copolymer (ABS), polycarbonate, polyoxymethylene, polytetrafluoroethylene, polypropylene, polyvinyl chloride, polyphenylene sulfide, nylon, silica gel, aluminum alloy, carbon steel, stainless steel, copper, etc.

[0040] In a specific embodiment of the present application, the uniform grid 108 is a filtering medium woven from natural fibers or synthetic fibers, and is used to spread the test solution on the uniform grid 108 to prevent the test solution from passing through a certain place of the sample adding hole 102 concentratedly.

[0041] In a specific embodiment of the present application, the flow guiding column 106 abuts against the through hole.

[0042] In a specific embodiment of the present application, the distance between the flow guiding column 106 and the through hole is 0 to 0.1 mm, for example, it can be 0 mm, 0.1 mm, 0.15 mm, 0.2 mm, 0.25 mm, 0.3 mm, 0.35 mm, 0.4 mm, 0.45 mm, 0.5 mm; a drainage angle 107 is provided between each flow guiding column 106 and the adjacent through hole, and the flow rate and flow volume of the liquid to be measured flowing into the adjacent through hole can be adjusted through the drainage angle 107.

[0043] In a specific embodiment of the present application, the drainage angle 107 is provided on the lower surface of the sample adding hole 102, between the flow guiding column 106 and its adjacent through hole. The drainage angle 107 abuts against the lower surface of the sample adding hole 102 and the flow guiding column 106 respectively. The angle of the drainage angle 107 facing the through hole forms an angle of 30 to 60° with the lower surface of the sample adding hole 102.

[0044] In a specific embodiment of the present application, the number of the through holes is odd. The aperture of the through hole in the middle is larger than that of the remaining through holes. The aperture of the through hole in the middle is 2.0 to 4.0 mm, for example, it can be 2.0 mm, 2.1 mm, 2.2 mm, 2.3 mm, 2.4 mm, 2.5 mm, 2.6 mm, 2.7 mm, 2.8 mm, 2.9 mm, 3.0 mm, 3.1 mm, 3.2 mm, 3.3 mm, 3.4 mm, 3.5 mm, 3.6 mm, 3.7 mm, 3.8 mm, 3.9 mm, 4.0 mm. The aperture of the other through holes is 1.5 to 3.5 mm, for example, it can be 1.5 mm, 1.6 mm, 1.7 mm, 1.8 mm, 1.9 mm, 2.0 mm, 2.1 mm, 2.2 mm, 2.3 mm, 2.4 mm, 2.5 mm, 2.6 mm, 2.7 mm, 2.8 mm, 2.9 mm, 3.0 mm, 3.1 mm, 3.2 mm, 3.3 mm, 3.4 mm, 3.5 mm.

[0045] In a specific embodiment of the present application, the number of the through holes is odd, and no flow guiding column 106 may be provided around the through hole in the middle.

[0046] In a specific embodiment of the present application, the number of through-holes is an even number, the apertures of all through-holes are uniform, and the aperture of the through-hole is 1.5 - 3.5 mm. For example, it can be 1.5 mm, 1.6 mm, 1.7 mm, 1.8 mm, 1.9 mm, 2.0 mm, 2.1 mm, 2.2 mm, 2.3 mm, 2.4 mm, 2.5 mm, 2.6 mm, 2.7 mm, 2.8 mm, 2.9 mm, 3.0 mm, 3.1 mm, 3.2 mm, 3.3 mm, 3.4 mm, 3.5 mm.

[0047] In a specific embodiment of the present application, the number of through-holes is an even number, and a flow guiding column 106 is arranged around each through-hole.

[0048] In a specific embodiment of the present application, in the direction extending from the upper housing towards the lower housing, the stepped structure is a frustum of a square pyramid structure with 2 - 5 levels of steps on both sides. For example, it can be 2 levels of steps, 3 levels of steps, 4 levels of steps, 5 levels of steps.

[0049] In a specific embodiment of the present application, a stepped structure is arranged between each adjacent through-hole.

[0050] In a specific embodiment of the present application, the height of each level of step is 0.1 - 2 mm. For example, it can be 0.1 mm, 0.2 mm, 0.3 mm, 0.4 mm, 0.5 mm, 0.6 mm, 0.7 mm, 0.8 mm, 0.9 mm, 1.0 mm, 1.1 mm, 1.2 mm, 1.3 mm, 1.4 mm, 1.5 mm, 1.6 mm, 1.7 mm, 1.8 mm, 1.9 mm, 2.0 mm.

[0051] In a specific embodiment of the present application, the maximum width of the stepped structure is 1 - 3 mm. For example, it can be 1.0 mm, 1.1 mm, 1.2 mm, 1.3 mm, 1.4 mm, 1.5 mm, 1.6 mm, 1.7 mm, 1.8 mm, 1.9 mm, 2.0 mm, 2.1 mm, 2.2 mm, 2.3 mm, 2.4 mm, 2.5 mm, 2.6 mm, 2.7 mm, 2.8 mm, 2.9 mm, 3.0 mm.

[0052] In a specific embodiment of the present application, the number of the observation windows 101 is the same as the number of the through-holes, and they are arranged in an array or side by side. The number of the through-holes is the same as the number of the test strips.

[0053] In a specific embodiment of the present application, a support groove 103 is arranged around the observation window 101 on the lower surface of the upper housing 1.

[0054] In a specific embodiment of the present application, a plurality of support bars 109 are further provided on the lower surface of the upper housing 1.

[0055] In a specific embodiment of the present application, the periphery of the test strip slot 201 can form a fixing slot 202 with at most three sides for fixing the test strip.

[0056] Next, in combination with specific embodiments, the immunochromatographic multi-channel sample quantitative detection device disclosed in the present application will be further specifically introduced.

[0057] Embodiment 1

[0058] As Figure 1 shown, this embodiment relates to an immunochromatographic multi-channel sample quantitative detection device, specifically including: an upper housing 1 and a lower housing 2. As Figure 2 shown, an observation window 101 and a sample addition hole 102 are provided on the upper surface of the upper housing 1. The sample addition hole 102 is elliptical when viewed from above, and the sample addition hole 102 is embedded in the upper housing 1. As Figure 4 Or Figure 5 shown, three through holes are evenly spaced on the lower surface of the sample addition hole 102, namely the first through hole 1021, the second through hole 1022, and the third through hole 1023. A diversion column 106 extending in the direction of the lower housing 2 (i.e., Figure 3 the Z direction shown) is provided on the lower surface of the sample addition hole 102. The diversion columns 106 are respectively arranged on one side of the first through hole 1021 and the third through hole 1023 close to the edge of the sample addition hole 102, and the outer surfaces of the two diversion columns 106 coincide with the edges of the first through hole 1021 and the third through hole 1023 respectively. Among the three through holes, the aperture of the second through hole 1022 is the largest, which is 4.0 mm, and the apertures of the first through hole 1021 and the third through hole 1023 are the same, both being 3.0 mm. Between two adjacent through holes, namely between the first through hole 1021 and the second through hole 1022, and between the second through hole 1022 and the third through hole 1023, there is a stepped structure 105 respectively. The structures of the two stepped structures 105 are exactly the same, both being 3-level steps. The height of each step is 1.0 mm, and the maximum width of each step is 0.6 mm, that is, the width of the first step is 0.6 mm, the second step extends 0.6 mm wide to the left and right from the bottom of the first step, and extends 1.0 mm high in the Z direction, and the third step extends 0.6 mm wide to the left and right from the bottom of the second step, and extends 1.0 mm high in the Z direction. There are 3 observation windows 101, which are arranged in parallel at a fixed interval and parallel to the Y direction on the upper surface of the upper housing 1. As Figure 4As shown in the figure, a support groove 103 is provided around the observation window 101 on the lower surface of the upper housing 1. Six support bars 109 are also provided on the lower surface of the upper housing 1, and are arranged in an array between the positions where the observation window 103 is opened and the position where the sample addition hole 102 is opened. A uniform grid 108 covering all three through holes is also provided in the sample addition hole 102. The uniform grid 108 is a filter medium made of synthetic fibers. A test strip groove 201 and a test strip holder 203 are provided on the upper surface of the lower housing 2. The test strip grooves 201 are arranged side by side parallel to the Y direction on the upper surface of the lower housing 2. The test strip holders 203 are arranged at equal intervals parallel to the X direction on the upper surface of the lower housing 2. The periphery of the test strip groove 201 can form a three-sided fixing groove 202 for fixing the test strip. A set of snap structures are provided at the relative positions of the lower surface of the upper housing 1 and the upper surface of the lower housing 2 for snapping the upper housing 1 and the lower housing 2 together.

[0059] Example 2

[0060] The difference between this example and Example 1 is that the distances between the two guide posts 106 and the first through hole 1021 and the third through hole 1023 are both 0.1 mm. At the same time, there are two drainage corners 107 on the lower surface of the sample addition hole 102, which are respectively arranged at the positions between the guide posts 106 and their adjacent through holes. The drainage corners 107 are respectively in contact with the lower surface of the sample addition hole 102 and the guide posts 106. The angle of the drainage corner 107 facing the through hole and the lower surface of the sample addition hole 102 is 30°.

[0061] Example 3

[0062] The difference between this example and Example 2 is that the angle of the drainage corner 107 facing the through hole and the lower surface of the sample addition hole 102 is 60°.

[0063] Example 4

[0064] The difference between this example and Example 1 is that both of the two stepped structures 105 are 2-level steps. The height of each step is 1.0 mm, and the maximum width of each step is 1.0 mm. That is, the width of the first step is 1.0 mm, and the second step extends 1.0 mm wide to the left and right respectively from the bottom of the first step and extends 1.0 mm high in the Z direction.

[0065] Example 5

[0066] The difference between this embodiment and Embodiment 1 is that both of the two stepped structures 105 are 4-level steps. The height of each step is 1.0 mm, and the maximum width of each step is 0.75 mm. That is, the width of the first step is 0.75 mm. The second step extends 0.75 mm wide to the left and right respectively from the bottom of the first step and extends 1.0 mm high in the Z direction. The third step extends 0.75 mm wide to the left and right respectively from the bottom of the first step and extends 1.0 mm high in the Z direction. The fourth step extends 0.75 mm wide to the left and right respectively from the bottom of the first step and extends 1.0 mm high in the Z direction.

[0067] Comparative Example 1

[0068] The difference between this embodiment and Embodiment 2 is that the angle of the drainage angle 107 facing the through hole forms an 80° angle with the lower surface of the sample addition hole 102.

[0069] Comparative Example 2

[0070] The difference between this embodiment and Embodiment 2 is that the drainage angle 107 is not provided.

[0071] Embodiment 6

[0072] The difference between this embodiment and Embodiment 1 is that two through holes are symmetrically arranged on the lower surface of the sample addition hole 102. A flow guiding column 106 extending in the direction of the lower housing 2 (i.e., Figure 3 the Z direction shown) is provided on the lower surface of the sample addition hole 102. The flow guiding columns 106 are respectively arranged on one side of the two through holes close to the edge of the sample addition hole 102, and the outer surfaces of the two flow guiding columns 106 respectively coincide with the edges of the two through holes. The apertures of the two through holes are the same, both being 2.0 mm. There are 2 observation windows 101, which are fixedly arranged side by side at a fixed interval on the upper surface of the upper housing 1.

[0073] Embodiment 7

[0074] The difference between this embodiment and Embodiment 6 is that the distances between the two flow guiding columns 106 and the through holes are both 0.1 mm. At the same time, there are two drainage angles 107 on the lower surface of the sample addition hole 102, which are respectively arranged at the positions between the flow guiding columns 106 and their adjacent through holes. The drainage angles 107 are respectively in contact with the lower surface of the sample addition hole 102 and the flow guiding columns 106. The angle of the drainage angle 107 facing the through hole forms a 30° angle with the lower surface of the sample addition hole 102.

[0075] Embodiment 8

[0076] The difference between this embodiment and Embodiment 6 is that the distances between the two flow guiding columns 106 and the through holes are both 0.1 mm. At the same time, there are two drainage corners 107 on the lower surface of the sample adding hole 102, which are respectively arranged at the positions between the flow guiding column 106 and its adjacent through hole. The drainage corners 107 are respectively in contact with the lower surface of the sample adding hole 102 and the flow guiding column 106, and the angle of the drainage corner 107 facing the through hole forms a 60° angle with the lower surface of the sample adding hole 102.

[0077] Embodiment 9

[0078] The difference between this embodiment and Embodiment 6 is that the stepped structure 105 is a two-stage step, the height of each step is 1.0 mm, and the maximum width of each step is 1.0 mm, that is, the width of the first step is 1.0 mm, and the second step extends 1.0 mm wide to the left and right respectively from the bottom of the first step and extends 1.0 mm high in the Z direction.

[0079] Embodiment 10

[0080] The difference between this embodiment and Embodiment 6 is that the stepped structures 105 are all four-stage steps, the height of each step is 1.0 mm, and the maximum width of each step is 0.75 mm, that is, the width of the first step is 0.75 mm, the second step extends 0.75 mm wide to the left and right respectively from the bottom of the first step and extends 1.0 mm high in the Z direction, the third step extends 0.75 mm wide to the left and right respectively from the bottom of the first step and extends 1.0 mm high in the Z direction, and the fourth step extends 0.75 mm wide to the left and right respectively from the bottom of the first step and extends 1.0 mm high in the Z direction.

[0081] Comparative Example 3

[0082] The difference between this embodiment and Embodiment 6 is that the distances between the two flow guiding columns 106 and the through holes are both 0.1 mm. At the same time, there are two drainage corners 107 on the lower surface of the sample adding hole 102, which are respectively arranged at the positions between the flow guiding column 106 and its adjacent through hole. The drainage corners 107 are respectively in contact with the lower surface of the sample adding hole 102 and the flow guiding column 106, and the angle of the drainage corner 107 facing the through hole forms an 80° angle with the lower surface of the sample adding hole 102.

[0083] Comparative Example 4

[0084] The difference between this embodiment and Embodiment 6 is that the drainage corners 107 are not provided.

[0085] Experimental Example 1

[0086] Prepare a test solution with a concentration of 30 μg / ml for standby. In the sample addition holes 102 of Examples 1-5 and Comparative Examples 1-2, add 300 μl of the test solution respectively. After the reaction time ends, use an instrument to measure the concentration of the test paper to verify the average flow splitting and quantification effect of the test solution. The measurement results are shown in Table 1.

[0087] Experimental Example 2

[0088] Prepare a test solution with a concentration of 30 μg / ml for standby. In the sample addition holes 102 of Examples 6-10 and Comparative Examples 3-4, add 200 μl of the test solution respectively. After the reaction time ends, use an instrument to measure the concentration of the test paper to verify the average flow splitting and quantification effect of the test solution. The measurement results are shown in Table 1.

[0089] Table 1 Measurement Results of Each Example and Comparative Example

[0090]

[0091]

[0092] Note: " / " indicates that this example does not involve this structure or result.

[0093] As shown in Table 1 of the experimental data, in Example 1, since the drainage angle 107 was not set, the test solution flowed directly down from the flow guiding column 106 at a relatively high flow rate. Holes 1 and 3 could effectively perform average flow splitting and quantification with hole 2, and the CV between holes was as low as 4.0%, with an excellent average flow splitting and quantification effect. In both Example 2 and Example 3, the drainage angle 107 was set, and the angle of the drainage angle 107 gradually increased. The CV between holes was higher than that in Example 1, and the average flow splitting and quantification effect was slightly lower than that in Example 1. In Example 4 and Example 5, 2-level steps and 4-level steps were respectively adopted. Due to the lack of spacing between the setting position of the flow guiding column 106 and the holes, the CV between holes was relatively low, and the average flow splitting and quantification effect was slightly lower than that in Example 1 but slightly higher than that in Example 4 and Example 5. In Comparative Example 1, a drainage angle 107 with too large an angle was set, and it did not achieve a good average flow splitting and quantification effect. The CV between holes was too high, and the average flow splitting and quantification effect was poor. In Comparative Example 2, there was a spacing between the flow guiding column and the holes and the drainage angle 107 was not set, which seriously affected the flow rate of the test solution in the through holes on both sides. The CV between holes was low, and the average flow splitting and quantification effect was poor.

[0094] In Examples 6-10, due to the small number of through holes, only two through holes, and the same through hole diameters, average flow splitting and quantification were relatively easy, and the CV between holes was relatively low. The CV between holes in Example 6 could reach 3.4%, with a quite excellent average flow splitting and quantification effect. In Comparative Example 3 and Comparative Example 4, due to setting a too large drainage angle 107 and the existence of a spacing between the flow guiding column 106 and the holes and the non-setting of the drainage angle 107 respectively, the CV between holes was relatively low, and the average flow splitting and quantification effect was poor.

[0095] By setting up four structures for uniform distribution of quantitative liquid, namely a uniformly distributed grid, a stepped structure, a diversion column and a drainage angle, in an immunochromatographic analysis multi-channel sample quantitative detection device, the effect of average shunt quantification can be effectively improved. By using the above four structures for uniform distribution of quantitative liquid to assist quantification, when using a multi-link card immunotest detection card, the average shunt quantification effect of the test liquid dropped into each test strip in the multi-link card can be improved.

[0096] The above-described embodiments merely represent several implementation manners of the present application, and the description thereof is relatively specific and detailed, but it should not be construed as a limitation on the scope of the present application. It should be noted that for those of ordinary skill in the art, without departing from the concept of the present application, several modifications and improvements can still be made, and these all belong to the protection scope of the present application. Therefore, the protection scope of the patent of the present application shall be subject to the appended claims.

Claims

1. An immunochromatographic multi-channel sample quantitative detection device, wherein, Comprising: Upper shell; And, Lower shell; An observation window and a sample addition hole are provided on the upper surface of the upper shell; The sample addition hole is embedded in the upper shell, and at least two through holes are evenly spaced on the lower surface of the sample addition hole; A diversion column extending towards the lower shell is provided on the lower surface of the sample addition hole; A uniform grid covering all the through holes is further provided in the sample addition hole; A stepped structure is provided in the space between the uniform grid and the lower surface of the sample addition hole, and is distributed between the through holes provided on the lower surface of the sample addition hole; A test strip slot and a test strip holder are provided on the upper surface of the lower shell; A set of snap structures are provided at the relative positions of the lower surface of the upper shell and the upper surface of the lower shell for snapping the upper shell and the lower shell together.

2. The device according to the claim, wherein, The diversion column abuts against the through hole.

3. The device according to the claim, wherein, The distance between the diversion column and the through hole is 0 to 0.5 mm, and an inlet angle is optionally provided between each diversion column and the adjacent through hole.

4. The apparatus according to claim 3, wherein, The angle of the inlet angle facing the through hole forms an angle of 30 to 60° with the lower surface of the sample addition hole.

5. The device according to any one of claims 1 to 4, wherein, The number of the through holes is odd, preferably the aperture of the middle through hole is larger than that of the remaining through holes, and further preferably no diversion column is provided around the middle through hole.

6. The device according to any one of claims 1 to 4, wherein The number of the through holes is even, preferably the apertures of the through holes are uniform, and further preferably a diversion column is provided around each through hole.

7. The apparatus according to claim 5, wherein, The aperture of the middle through hole is 2.0 to 4.0 mm; the apertures of the remaining through holes are 1.5 to 3.5 mm.

8. The apparatus according to claim 6, wherein, The apertures of the through holes are all 1.5 to 3.5 mm.

9. The device according to any one of claims 1 to 8, wherein, In the direction extending from the upper shell towards the lower shell, the stepped structure is a frustum structure with 2 to 5 levels of steps on both sides.

10. The apparatus according to claim 9, wherein, A stepped structure is provided between every two adjacent through holes.

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