Compound protein nutrition powder capable of improving satiety and resisting fatigue and preparation method of compound protein nutrition powder

Abstract

The invention discloses compound protein nutrition powder capable of improving satiety and resisting fatigue and a preparation method of the compound protein nutrition powder, and belongs to the technical field of nutritional meal replacement food. The compound protein nutrition powder comprises an anti-fatigue composition, and further comprises at least one of psyllium seed husks, chia seeds, whey protein, soy isolate protein, a sweetening agent, a flavoring agent, an antagonist and a stabilizer. The anti-fatigue composition comprises a flastem milkvetch seed extract, a ligusticum wallichii extract, an agaricus bisporus extract, a citrus extract and a rhizoma polygonati extract. According to the anti-fatigue composition provided by the invention, the anti-fatigue performance is remarkably improved through the synergistic effect of multiple components; the compound protein nutrition powder provided by the invention has double effects of resisting fatigue and satiety, is efficient and stable in process, and is suitable for the fields of sports nutrition supplement and healthy meal replacement.

Description

Technical Field

[0001] The invention belongs to the technical field of nutritious meal replacement foods, and specifically relates to a composite protein nutritious powder capable of improving satiety and resisting fatigue, and a preparation method thereof. Background Art

[0002] In view of the growing demand for nutritious meal replacement foods that are both anti-fatigue and satiety-enhancing among people who do high-intensity exercise, people under high pressure in the workplace, chronic fatigue patients, and weight management personnel, it is of great significance to develop a compound protein nutritional powder that can significantly fight fatigue and enhance satiety.

[0003] However, the existing anti-fatigue products mostly rely on a single active ingredient (such as caffeine, ginseng extract, etc.), which has problems such as short-term effects and easy tolerance; while traditional satiety formulas are often achieved through high fiber or high protein, but often ignore the synergistic effect of energy metabolism and fatigue relief, resulting in insufficient duration of satiety and inability to effectively relieve post-exercise fatigue. In addition, the preparation process of existing composite nutritional powders is complicated, the extraction efficiency of active ingredients is low, and it is easy to cause the loss of functional ingredients. Summary of the invention

[0004] In view of the deficiencies in the prior art, the object of the present invention is to provide a composite protein nutritional powder with significant synergistic effect of ingredients, dual improvement of anti-fatigue and satiety, and efficient preparation process.

[0005] In order to achieve the above object, the present invention discloses the following technical solutions:

[0006] In a first aspect, the present invention provides an anti-fatigue composition, which comprises the following components in parts by mass:

[0007]

[0008] Preferably, the preparation method of the Astragalus complanatus extract comprises the following steps:

[0009] Step 1-1. Take a certain amount of dried seeds of Astragalus complanatus, grind them into powder, and pass them through a sieve of ≥40 mesh to obtain Astragalus complanatus powder;

[0010] Step 1-2. Mix the powder of Astragalus complanatus with deionized water at a mass volume ratio of 1: (4-6) g / mL, and add the composite enzyme preparation at an amount of 2-4% of the total mass of the liquid, and perform enzymolysis at a speed of 50-100 r / min while stirring for 24-36 h. After the enzymolysis is completed, heat to 95-100 ° C and keep warm for 15-20 min to inactivate the enzyme to obtain an enzymolyzate;

[0011] Step 1-3. Place the enzymatic hydrolysate in a heat reflux extraction device, and simultaneously add an ethanol aqueous solution with a concentration of 85 v / v%. The mass-to-volume ratio of the material to the liquid is 1:5-7 g / mL. Reflux and extract at 65-75°C for 2-4 times, each time for 0.5-0.7 h. Combine the extracts, concentrate the extracts under reduced pressure until there is no alcohol smell, and then perform spray drying. The drying conditions are an inlet air temperature of 175-185°C and an outlet air temperature of 70-85°C to obtain the semen astragali complanati extract.

[0012] Further preferably, the complex enzyme preparation contains cellulase, pectinase, and glucanase. The cellulase activity ≥ 1000 U / g, the pectinase activity ≥ 5000 U / g, and the glucanase activity ≥ 200 U / g;

[0013] Preferably, the preparation method of the ligusticum wallichii extract comprises the following steps:

[0014] Step 2-1. Take a certain amount of ligusticum wallichii rhizomes, wash them, slice them, dry them at 55-65°C until constant weight, and then crush and sieve them through a sieve with a mesh size of ≥ 40 meshes to obtain ligusticum wallichii powder;

[0015] Step 2-2. Place the ligusticum wallichii powder obtained in Step 2-1 in a heat reflux extraction device, and simultaneously add deionized water to the device. The mass-to-volume ratio of the material to the liquid is 1:(10-15) g / mL. Reflux and extract at 100°C for 2-3 times, each time for 1.5-2 hours, and combine the extracts;

[0016] Step 2-3. Concentrate the extract under reduced pressure at 70-80°C to a relative density of 1.10-1.15, and then perform spray drying. The drying conditions are an inlet air temperature of 175-185°C and an outlet air temperature of 70-85°C to obtain the ligusticum wallichii extract.

[0017] The semen astragali complanati extract is rich in active ingredients such as flavonoids, polysaccharides, saponins, amino acids, proteins, trace elements, and vitamins, and has the effects of antioxidant damage, activating immune cells, anti-inflammatory, and promoting blood circulation. In the present invention, a complex enzyme preparation is used to enzymatically hydrolyze semen astragali complanati, so that its active substances are fully released, the content of active substances in the semen astragali complanati extract is increased, and its effect is improved.

[0018] The ligusticum wallichii extract has the effects of dilating blood vessels, promoting blood circulation, and improving microcirculation. It accelerates the delivery of oxygen and the clearance of metabolic wastes (such as lactic acid), and at the same time inhibits inflammatory factors and regulates neurotransmitters, reduces muscle inflammation and central fatigue, and improves the recovery efficiency.

[0019] The extract of Agaricus bisporus is rich in polysaccharides and selenium, the extract of citrus fruits has a high content of vitamin C, the extract of Polygonatum sibiricum can enhance immunity, relieve physical exhaustion, help improve memory, and delay physical aging. The combined use of the extracts of Astragalus complanatus, Ligusticum chuanxiong, Agaricus bisporus, citrus fruits, and Polygonatum sibiricum achieves the effect of multi-channel synergistic anti-fatigue.

[0020] In a second aspect, the present invention provides a compound protein nutritional powder for enhancing satiety and anti-fatigue, and the compound protein nutritional powder contains the anti-fatigue composition according to any one of claims 1-4.

[0021] Preferably, the addition amount of the anti-fatigue composition in the compound protein nutritional powder is 5-10 wt%.

[0022] Preferably, the compound protein nutritional powder further contains at least one of psyllium husk, chia seeds, whey protein, soy protein isolate, sweetener, edible flavor, antagonist, and stabilizer.

[0023] In a third aspect, the present invention provides a preparation method of the compound protein nutritional powder according to the second aspect, and the preparation method includes the following steps

[0024] Step 3-1. Ultrafinely pulverize psyllium husk and chia seeds through a sieve with a mesh size of ≥120 meshes to obtain ultrafine powder of psyllium husk and ultrafine powder of chia seeds for standby;

[0025] Step 3-2. Add whey protein powder and soy protein isolate powder to a three-dimensional mixer, mix at 3-10 r / min for 10-15 min, then add the pre-mixed anti-fatigue composition, and continue to mix at 3-10 r / min for 15-20 min until uniform to obtain a preliminary premix;

[0026] Step 3-3. Transfer the preliminary premix to a double-motion mixer, add the ultrafine powder of psyllium husk and the ultrafine powder of chia seeds, mix at a barrel rotation speed of 20-30 r / min and an inner paddle rotation speed of 40-60 r / min for 20-30 min, then add the sweetener, anti-caking agent, edible flavor, and stabilizer, mix at a barrel rotation speed of 20-30 r / min and an inner paddle rotation speed of 40-60 r / min for 20-30 min, and finally fill after passing through a vibration sieve with a mesh size of ≥80 meshes to obtain the compound protein nutritional powder.

[0027] The beneficial effects of the present invention:

[0028] 1. Through the synergistic effect of the extracts of Astragalus complanatus, Ligusticum chuanxiong, Agaricus bisporus, citrus fruits, and Polygonatum sibiricum, the present invention significantly improves the rod rotation endurance time, exhaustive swimming time, and glycogen level of mice, and effectively delays fatigue;

[0029] 2. The compound protein nutritional powder provided by the present invention has an obvious effect of enhancing satiety, and can provide a more lasting hunger resistance effect for consumers under the condition of lower calories. Detailed implementation manners

[0030] The present invention will be further described below in conjunction with specific embodiments, and the advantages and characteristics of the present invention will become clearer as the description proceeds. However, these embodiments are only exemplary and do not constitute any limitation to the scope of the present invention.

[0031] Those skilled in the art should understand that the details and forms of the technical solutions of the present invention can be modified or replaced without departing from the spirit and scope of the present invention, but these modifications and replacements all fall within the protection scope of the present invention.

[0032] In the present invention:

[0033] Astragalus complanatus: Phyllolobium chinense Fisch.ex DC., belonging to the genus Phyllolobium of the Leguminosae family;

[0034] Ligusticum wallichii Franch.: belonging to the genus Ligusticum of the Umbelliferae family (Ligusticum sinense 'Chuanxiong');

[0035] The raw materials and reagents used in the embodiments of the present invention are all commercially available.

[0036] Preparation of Astragalus complanatus extract

[0037] Step 1-1. Take a certain amount of dried seeds of Astragalus complanatus, grind them into powder, and pass through a sieve with a mesh size of ≥40 meshes to obtain Astragalus complanatus powder;

[0038] Step 1-2. Mix the Astragalus complanatus powder with deionized water at a mass-to-volume ratio of 1:5 g / mL, and at the same time add a compound enzyme preparation, with an addition amount of 3% of the total mass of the feed liquid. Stir and enzymatically hydrolyze for 24 h at a rotation speed of 80 r / min. After the enzymatic hydrolysis is completed, heat to 95 °C and keep warm for 15 min to inactivate the enzyme, obtaining an enzymatically hydrolyzed product;

[0039] Among them, the compound enzyme preparation contains cellulase, pectinase and glucanase. The enzyme activity of cellulase in the compound enzyme preparation product label is ≥1000 U / g, the enzyme activity of pectinase is ≥5000 U / g, and the enzyme activity of glucanase is ≥200 U / g. The factory inspection report shows that the enzyme activity of cellulase is 1332 U / g, the enzyme activity of pectinase is 5764 U / g, and the enzyme activity of glucanase is 297 U / g;

[0040] Step 1-3. Place the enzymatic hydrolysate in a heat reflux extraction device, and simultaneously add an ethanol aqueous solution with a concentration of 85 v / v%. The mass-to-volume ratio of the material to the liquid is 1:6 g / mL. Reflux extract at 70°C for 3 times, 0.6 h each time. Combine the extract solutions, concentrate the extract under reduced pressure until the alcohol smell disappears, and then perform spray drying. The drying conditions are an inlet air temperature of 180°C and an outlet air temperature of 80°C to obtain the semen astragali complanati extract.

[0041] Preparation of Ligusticum wallichii extract

[0042] Step 2-1. Take a certain amount of Ligusticum wallichii rhizomes, wash them, slice them, place them in an oven, dry them to constant weight at 60°C, and after drying, crush them and pass through a 40-mesh sieve to obtain Ligusticum wallichii powder.

[0043] Step 2-2. Place the Ligusticum wallichii powder obtained in Step 2-1 in a heat reflux extraction device, and simultaneously add deionized water to the device. The mass-to-volume ratio of the material to the liquid is 1:13 g / mL. Reflux extract at 100°C for 2 times, 1.5 hours each time, and combine the extract solutions.

[0044] Step 2-3. Concentrate the extract under reduced pressure to a relative density of 1.12 at 70°C, and then perform spray drying. The drying conditions are an inlet air temperature of 180°C and an outlet air temperature of 80°C to obtain the Ligusticum wallichii extract.

[0045] Anti-fatigue composition

[0046] Compound according to the raw material mass ratio in Table 1 to obtain the anti-fatigue composition.

[0047] Table 1 Mass ratio of anti-fatigue composition

[0048] Raw material name Composition 1 Composition 2 Composition 3 Composition 4 Astragalus complanatus extract 0.5 0.7 1 - Chuanxiong extract 1 2 4 2 Agaricus bisporus extract 13 15 17 15 Citrus extract 5 6 7 6 Polygonatum sibiricum extract 3 3 4 3 Raw material name Composition 5 Composition 6 Composition 7 Composition 8 Astragalus complanatus extract 0.7 0.7 0.7 1 Chuanxiong extract - 2 2 1 Agaricus bisporus extract 15 15 15 1 Citrus extract 6 - 6 1 Polygonatum sibiricum extract 3 3 - 1

[0049] Performance test

[0050] Anti-fatigue performance test

[0051] The experimental BALB / C mice are female, with a body weight of 20±2 g. The test temperature is 20-24°C, and the relative humidity is 40-70%. The animals are acclimated in the animal house environment for 4 days before the test. Oral administration of different doses of the test solution is given continuously for 30 days. The negative control group is given the same volume of normal saline, and the remaining treatments are the same as the dose groups, with 10 mice in each group.

[0052] 1 Treatment of test materials

[0053] The test samples are Composition 1-8. The recommended daily dose of the test samples for humans is 8 g. Calculated according to an adult body weight of 60 kg, the recommended dose of the sample is 0.133 g / kg·d.

[0054] (1) For the experiments of Compositions 1 - 3, three dose groups were set respectively. The low, medium, and high doses were 0.133 g / kg, 0.667 g / kg, and 1.333 g / kg of the test samples, which were equivalent to 1, 5, and 10 times the recommended dose;

[0055] (2) For Compositions 4 - 8, one dose group was set respectively, and a comparative experiment was conducted with Composition 2. The daily administration dose of Compositions 4 - 8 was the same as that of the medium - dose group of Composition 2, which was 0.667 g / kg;

[0056] (3) A negative control group was set, and the mice in the negative control group were given the same volume of normal saline.

[0057] Before the experiment, the above - mentioned test samples were formulated into test solutions of each dose with normal saline as the solvent, and were homogenized and dispersed evenly at 10000 r / min for testing; the obtained test solutions were administered by gavage, and the gavage volume was 0.8 mL per mouse.

[0058] 2 Determination of the rotarod endurance time of mice

[0059] There were 10 mice in each group. After 3 weeks of administration, one - week adaptive training was carried out, about 30 min each time. Mice that jumped, curled up around the axis, or were not good at climbing were excluded during the experiment. On the 28th day, 6 mice were selected from each group as the formal test animals. The mice were placed on a continuously rotating rotarod respectively. The starting rotation speed was 5 r / min, the final rotation speed was 30 r / min, the acceleration time was 50 s, and the mice moved in the opposite direction of rotation. The mice gradually entered a state of fatigue until they finally fell off the rotarod. The rotarod endurance time of each mouse was recorded respectively. The results are shown in Table 2.

[0060] 3 Determination of the exhaustive swimming time of mice

[0061] On the 30th day of administration, which was the last administration, 30 min after gavage, 6 mice were randomly selected and placed in a swimming tank to swim. The water depth was about 40 cm, the water temperature was maintained at about 25 ± 5 °C, and a lead block with a relative body mass of about 5% was fixed to the tail. The exhaustive swimming time was based on the time when the mouse swam until its head was completely submerged in the water for 10 s and no longer floated up. The exhaustive swimming time of each group of mice was recorded in time. The results are shown in Table 2.

[0062] 4 Determination of the levels of muscle glycogen and liver glycogen in mice

[0063] After the exhaustive swimming experiment, the remaining 4 mice in each group were anesthetized with ether and sacrificed by dislocation. 150 mg of liver and thigh muscle tissues were taken respectively and added to containers containing normal saline. They were ground using a high-speed homogenizer at a rotation speed of 20,000 r / min to obtain 10% tissue homogenate. Then, they were centrifuged at 7,000 r / min for 15 min to obtain the supernatant. Then, strictly following the kit instructions, the liver glycogen and muscle glycogen levels were measured respectively. The liver glycogen and muscle glycogen detection kits were purchased from Keaibo Biotechnology. The results are shown in Table 2.

[0064] 5 Statistical methods

[0065] SPSS 19.0 was used for statistical analysis. The rotarod endurance time, exhaustive swimming time, liver glycogen, and muscle glycogen levels of each group of mice were recorded, all expressed as , and one-way ANOVA was used. P < 0.05 indicated that the difference was statistically significant.

[0066] 6 Experimental results

[0067] Table 2 Anti-fatigue experiment results of each group

[0068]

[0069]

[0070] Note: "a" indicates significant difference compared with the negative control group, P < 0.05, "aa" indicates P < 0.05; "b" indicates significant difference between the medium-dose group of Composition 2 and Compositions 4 - 8, P < 0.05, "bb" indicates P < 0.05

[0071] 7 Result analysis

[0072] According to the experimental data in Table 2, there were significant differences in the anti-fatigue effects of different compositions. The following is a specific analysis:

[0073] Dose-effect of Compositions 1 - 3

[0074] In the low-, medium-, and high-dose groups of Compositions 1 - 3, the rotarod endurance time, exhaustive swimming time, muscle glycogen, and liver glycogen levels were all significantly higher than those of the negative control group (P < 0.05), and showed a dose-dependent increasing trend. For example, the rotarod endurance time and exhaustive swimming time of the high-dose group of Composition 2 were significantly better than those of the low- and medium-dose groups. In addition, the glycogen levels also showed an upward trend with the increase in dose, further indicating that the anti-fatigue composition provided by the present invention alleviates fatigue by enhancing energy reserve.

[0075] Comparison between Compositions 4 - 8 and Composition 2

[0076] The anti-fatigue effects of Compositions 4 - 8 were overall weaker than those of the medium-dose group of Composition 2. For example, the rotarod endurance time, exhaustive swimming time, and glycogen level of Composition 4 (without semen astragali complanati extract) were significantly lower than those of the medium-dose group of Composition 2 (P < 0.05). Compared with the formula of Composition 2, the formulas of Compositions 5 - 7 lacked chuanxiong rhizome extract, citrus extract, and polygonatum sibiricum extract respectively. The rotarod endurance time, exhaustive swimming time, and glycogen level of Compositions 5 - 7 also showed significant decreases compared with the anti-fatigue indexes of the medium-dose group of Composition 2, indicating that the absence of semen astragali complanati extract, chuanxiong rhizome extract, agaricus bisporus extract, citrus extract, and polygonatum sibiricum extract affected the anti-fatigue effect of the overall formula. The rotarod endurance time, exhaustive swimming time, and glycogen level of Composition 8 were better than those of other Compositions 4 - 7, but still not as good as those of Composition 2, suggesting that the ratio of each component in the formula also played a key role in the formula.

[0077] Analysis of the Synergistic Effect of Ingredients

[0078] The mass ratio of semen astragali complanati extract to chuanxiong rhizome extract in Compositions 1 - 3 was 0.5 - 1:1 - 4, which enhanced the anti-fatigue effect through multi-target synergistic effects. The flavonoid components of semen astragali complanati reduced exercise fatigue through the antioxidant pathway, the active ingredients of chuanxiong improved microcirculation, and the polysaccharides, vitamins, saponins and other components in agaricus bisporus, citrus, and polygonatum sibiricum extracts further played a role by regulating energy metabolism and glycogen synthesis. In contrast, the absence or imbalance of certain components in Compositions 4 - 8 led to a weakened synergistic effect.

[0079] Association between Glycogen Level and Anti-Fatigue Mechanism

[0080] The experimental results showed that excellent anti-fatigue compositions were all accompanied by a significant increase in muscle glycogen and liver glycogen levels. As an important energy source during exercise, the increase in glycogen reserve could delay the occurrence of fatigue, which was directly related to the extension of the exhaustive swimming time. In addition, the increase in liver glycogen level further supported the energy demand for long-term exercise by maintaining blood glucose stability.

[0081] Compound Protein Nutritional Powder with Enhanced Satiety

[0082] Accurately weigh the raw materials according to the mass percentages in the table;

[0083] Step 3 - 1. Ultrafinely crush psyllium husk and chia seeds and pass through a sieve of ≥120 mesh to obtain ultrafine powder of psyllium husk and chia seeds, ensuring uniform mixing;

[0084] Step 3 - 2. Add whey protein powder and soy protein isolate powder to a three-dimensional mixer, mix at 8 r / min for 10 min, then add the pre-mixed anti-fatigue composition, and continue to mix at 8 r / min for 15 min until uniform to obtain a preliminary premix;

[0085] Step 3-3. Transfer the primary premix to a double-motion mixer, add psyllium husk powder and chia seed powder, mix for 20 minutes at a barrel speed of 20 r / min and an inner blade speed of 50 r / min, then add sweeteners, anticaking agents, edible flavors and stabilizers, mix for 20 minutes at a barrel speed of 20 r / min and an inner blade speed of 50 r / min, and finally pass through an 80-mesh vibrating screen for filling to obtain a compound protein nutritional powder.

[0086] Table 3 Mass percentage of each raw material of compound protein nutritional powder

[0087]

[0088]

[0089] Satiety test

[0090] This experiment adopted a three-period crossover trial design and recruited 20 healthy male volunteers to evaluate the effect of dietary intervention.

[0091] Each participant was required to complete the experience of different breakfast plans in 2 independent tests, including two meal replacement tests for the experimental group (formula of Example 1) and the control group (commercially available rice cereal formula). All subjects were required to complete a crossover trial of the two formulas, with a 7-day washout period between each test to ensure the elimination of the residual effects of the previous intervention.

[0092] Diet plan: The subjects fasted from 20:00 the day before the test and had a standardized breakfast at 8:00 the next day. The energy supply of the test meal is shown in the following table, and it is required to complete the quantitative meal within 15 minutes.

[0093] Table 4 Energy of test meal

[0094]

[0095] Evaluation Methodology:

[0096] The feeling of fullness was measured uniformly 3 hours after the meal, using the internationally accepted visual analogue rating scale (VAS) for evaluation.

[0097] The scale consists of two 100 mm horizontal scale lines, with opposite feelings marked at both ends (e.g. 0 scale "no hunger" to 100 scale "extreme hunger"). The subjects need to mark the corresponding scale position according to their real-time feelings, and finally calculate the average length of the marked point from the left end of the scale (maximum 100.0 points) for quantitative analysis, as follows:

[0098] The content of the scale is as follows: (1) Hunger: The degree of hunger felt currently, with a scale of 0 - 100 mm, where 0 = no feeling at all and 100 = extremely intense; (2) Satiety: The degree of fullness in the stomach currently, with a scale of 0 - 100 mm, where 0 = no feeling at all and 100 = extremely intense. Through data collection and statistical processing of the marked distances of the subjects, the t-test is used for the analysis of significant differences. P < 0.05 indicates significant differences, and P < 0.01 indicates extremely significant differences. The results are shown in Table 5.

[0099] Experimental results:

[0100] Table 5 Comparison of hunger / satiety scores of volunteers 3 hours after consumption

[0101]

[0102]

[0103] Note: "*" indicates that there are statistically significant differences between the experimental group and the control group, P < 0.05.

[0104] Result analysis:

[0105] As can be seen from Table 5, the satiety score of the example group is significantly higher than that of the control group, and the hunger score of the example group is significantly lower than that of the comparison group. The difference is statistically significant (P < 0.05), indicating that the compound protein nutritional powder provided by the present invention has an obvious effect of enhancing satiety. Compared with commercially available regular breakfasts, it can provide a more lasting hunger resistance effect for consumers under the condition of lower calories.

[0106] Although the specific implementation manners of the present invention have been described above, those skilled in the art should understand that this is only an example. The protection scope of the present invention is defined by the appended claims. Without departing from the principles and essence of the present invention, those skilled in the art can make various changes or modifications to these implementation manners, but these changes and modifications all fall within the protection scope of the present invention.

Claims

1. An anti-fatigue composition, characterized in that, By mass parts, the composition comprises the following components:

2. The composition according to claim 1, wherein The preparation method of the semen astragali complanati extract comprises the following steps: Step 1-1. Take a certain amount of dried semen astragali complanati seeds, grind them into powder, and pass through a sieve with a mesh size of ≥40 meshes to obtain semen astragali complanati powder; Step 1-2. Mix the semen astragali complanati powder with deionized water at a mass-to-volume ratio of 1:(4-6) g / mL, and simultaneously add a composite enzyme preparation with an addition amount of 2-4% of the total mass of the feed liquid. Stir and enzymatically hydrolyze at a rotation speed of 50-100 r / min for 24-36 h. After the enzymatic hydrolysis is completed, heat to 95-100 °C and keep warm for 15-20 min to inactivate the enzyme, obtaining an enzymolysate; Step 1-3. Place the enzymolysate in a heat reflux extraction device, and simultaneously add an ethanol aqueous solution with a concentration of 85 v / v%. The mass-to-volume ratio of the feed liquid is 1:5-7 g / mL. Reflux extract at 65-75 °C for 2-4 times, each time for 0.5-0.7 h. Combine the extraction liquids, concentrate the extraction liquids under reduced pressure until there is no alcohol smell, and then perform spray drying. The drying conditions are an inlet air temperature of 175-185 °C and an outlet air temperature of 70-85 °C to obtain the semen astragali complanati extract.

3. The composition according to claim 2, wherein The composite enzyme preparation contains cellulase, pectinase, and glucanase. The enzyme activity of cellulase is ≥1000 U / g, the enzyme activity of pectinase is ≥5000 U / g, and the enzyme activity of glucanase is ≥200 U / g.

4. The composition according to claim 1, wherein The preparation method of the chuanxiong rhizome extract comprises the following steps: Step 2-1. Take a certain amount of chuanxiong rhizome, wash it, slice it, dry it to a constant weight at 55-65 °C, and after drying, crush it and pass through a sieve with a mesh size of ≥40 meshes to obtain chuanxiong powder; Step 2-2. Place the chuanxiong powder obtained in Step 2-1 in a heat reflux extraction device, and simultaneously add deionized water to the device. The mass-to-volume ratio of the feed liquid is 1:(10-15) g / mL. Reflux extract at 100 °C for 2-3 times, each time for 1.5-2 hours, and combine the extraction liquids; Step 2-3. Concentrate the extraction liquid under reduced pressure at 70-80 °C to a relative density of 1.10-1.15, and then perform spray drying. The drying conditions are an inlet air temperature of 175-185 °C and an outlet air temperature of 70-85 °C to obtain the chuanxiong rhizome extract.

5. A compound protein nutritional powder for enhancing satiety and anti-fatigue, characterized in that, The composite protein nutritional powder contains the anti-fatigue composition according to any one of claims 1-4.

6. The composite protein nutritional powder according to claim 5, wherein The addition amount of the anti-fatigue composition in the composite protein nutritional powder is 5-10 wt%.

7. The compound protein nutritional powder according to claim 6, characterized in that, The composite protein nutritional powder further contains at least one of psyllium husk, chia seeds, whey protein, soy protein isolate, sweetener, edible flavor, antagonist, and stabilizer.

8. The preparation method of the composite protein nutritional powder according to claim 7, characterized in that, The preparation method comprises the following steps Step 3-1. Ultrafinely crush psyllium husk and chia seeds and pass through a sieve with a mesh size of ≥120 meshes to obtain ultrafine psyllium husk powder and ultrafine chia seed powder for standby; Step 3-2. Add whey protein powder and soy protein isolate powder to a three-dimensional mixer, mix at 3-10 r / min for 10-15 min, and then add the pre-mixed anti-fatigue composition and continue to mix at 3-10 r / min for 15-20 min until uniform to obtain a preliminary premix; Step 3-3. Transfer the preliminary premix to a double-motion mixer, add psyllium husk ultrafine powder and chia seed ultrafine powder, mix for 20-30 minutes at a barrel rotation speed of 20-30 r / min and an inner paddle rotation speed of 40-60 r / min, then add sweeteners, anticaking agents, edible flavors and stabilizers, mix for 20-30 minutes at a barrel rotation speed of 20-30 r / min and an inner paddle rotation speed of 40-60 r / min, and finally fill after passing through a vibrating screen with a mesh size of ≥80 meshes to obtain the compound protein nutritional powder.

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