Moisturizing and anti-wrinkle composition containing double glucan, preparation method of moisturizing and anti-wrinkle composition and application of moisturizing and anti-wrinkle composition to cosmetics

Abstract

The invention belongs to the technical field of cosmetics, and particularly relates to a moisturizing and anti-wrinkle composition containing double glucan, a preparation method of the moisturizing and anti-wrinkle composition and application of the moisturizing and anti-wrinkle composition to cosmetics. The composition is prepared from the following components: glucan, beta-glucan, a kelp extract, butanediol, 1, 2-hexanediol and deionized water. The composition has good moisturizing and anti-wrinkle effects and has a good application prospect in cosmetics.

Description

Technical Field

[0001] The present invention belongs to the technical field of cosmetics, and particularly relates to a moisturizing and anti-wrinkle composition containing double glucans, a preparation method thereof, and an application thereof in cosmetics. Background Art

[0002] Anti-wrinkle and moisturizing are common efficacy requirements in cosmetics. Anti-wrinkle means anti-oxidation. The reasons for the appearance of wrinkles are mainly divided into two categories: internal aging (natural oxidation) and external aging (photoaging, smoking, alcoholism, poor diet, etc.). Generally speaking, the skin on the inner side of the arm basically reflects the progress of natural aging; if the skin on the face is worse than the skin on the inner side of the arm, showing uneven skin color, sagging and wrinkling, etc., it is all caused by external aging, especially photoaging caused by insufficient sun protection, accounting for more than 80%. Moisturizing refers to the moisturizing of the skin. When the air temperature drops, the stratum corneum of the skin cannot timely regulate sufficient moisturizing factors, the activity of the sebaceous glands decreases, the secretion of oil and moisture on the face decreases, and the skin will show a tight phenomenon, and even fine lines will appear under the eyes and beside the nose. The process of moisturizing the skin by using corresponding skin care and skin nourishing products, preventing the evaporation of skin moisture, improving blood microcirculation, and enhancing the skin moisture level is called moisturizing. The best moisturizing is the good interaction between the skin and the water replenishing products.

[0003] Anti-wrinkle and moisturizing products mainly play a role by moisturizing the skin, fading wrinkles and enhancing skin elasticity, but the effects are limited and vary from person to person. Existing anti-wrinkle and moisturizing products contain various nutritional ingredients, such as collagen peptides, hyaluronic acid, etc. These ingredients can help the skin retain moisture and increase the moisture content of the skin. By continuous use, anti-wrinkle and moisturizing products can reduce dry lines and fine lines on the skin surface to a certain extent, making the skin look smoother.

[0004] As a natural moisturizer, glucan can absorb moisture in the air to provide moisture for the skin, form a moisturizing barrier, maintain the moisture level of the skin, and avoid fine lines and skin aging caused by dryness. Glucan can form a protective film to lock in the skin moisture, and can also increase the softness and elasticity of the skin, making the skin healthier and younger.

[0005] There are also patent applications using glucan in skin care products in the prior art. For example, CN 114983894A discloses an anti-acne skin care product containing an anti-acne composition and a preparation method thereof. The anti-acne composition includes encapsulated salicylic acid, paeonol, sophora flavescens root extract, piroctone olamine, ecological nutrient solution, hydroxyethylpiperazine ethanesulfonic acid, β-glucan and centella asiatica extract. The anti-acne composition can efficiently and gently remove acne, control oil, reduce inflammation, and also enhance the skin repair function, promote the recovery of acne marks, remove acne scars and brighten the skin color. Although this patent application discloses the acne-removing effect of its composition, it does not disclose its moisturizing and anti-wrinkle efficacy. Summary of the Invention

[0006] To overcome the above technical problems, the present invention provides a composition, which has good anti-wrinkle and moisturizing effects and can be applied in cosmetics or skin care products, showing good application prospects.

[0007] To achieve the above object, the technical solution provided by the present invention is as follows:

[0008] A composition, comprising the following components: dextran, β-dextran, kelp extract, butylene glycol, 1,2-hexanediol, and deionized water.

[0009] Preferably, by weight, it comprises the following components: 0.1-1 part of dextran, 0.1-1 part of β-dextran, 0.1-0.5 part of kelp extract, 10-50 parts of butylene glycol, 1-5 parts of 1,2-hexanediol, and 60-120 parts of deionized water.

[0010] Preferably, by weight, it comprises the following components: 0.1-0.5 part of dextran, 0.1-0.5 part of β-dextran, 0.2-0.5 part of kelp extract, 10-20 parts of butylene glycol, 1-2 parts of 1,2-hexanediol, and 60-100 parts of deionized water.

[0011] Preferably, the weight ratio of dextran to β-dextran is 0.1-1:1; preferably 1:1.

[0012] Preferably, the weight ratio of dextran, β-dextran to kelp extract is 0.1-1:0.1-1:0.1-0.5; preferably 0.5:0.5:0.2.

[0013] Preferably, the composition may further contain ectoin, and the weight ratio of ectoin to kelp extract is 0.1:0.1-0.5.

[0014] The present invention also aims to provide a preparation method of the composition, comprising the following steps: adding dextran, β-dextran, and kelp extract together into butylene glycol and 1,2-hexanediol for infiltration and dispersion, then adding them together into deionized water, heating, stirring until completely dissolved, cooling down, and filtering with a 300-mesh filter cloth to obtain the composition.

[0015] Preferably, the heating temperature is 50-90°C, preferably 85°C.

[0016] Preferably, the stirring rate is 30-100 revolutions per minute.

[0017] Preferably, the filtering is carried out at a temperature below 38°C.

[0018] The present invention also aims to provide the application of the composition in cosmetics or skin care products.

[0019] The object of the present invention also lies in providing a cosmetic or skin care product, which comprises any one of the said compositions.

[0020] Compared with the prior art, the technical advantages of the present invention are as follows:

[0021] (1) The present invention provides a novel composition which contains dual glucans, effectively increasing the efficacy of glucans in terms of moisturization. Meanwhile, the addition of kelp extract enables good synergistic effects between the two and the kelp extract, effectively enhancing the anti-wrinkle and moisturizing efficacy of the composition, and also having good soothing and anti-allergic effects.

[0022] (2) Compared with traditional compositions, the present invention uses dual glucans of glucan and β-glucan, which have good complementary effects and effectively synergize with the kelp extract; there are good synergistic effects among the components, significantly enhancing the moisturizing and anti-wrinkle efficacy of the composition.

[0023] (3) Ectoin can also be added in the present invention, which can effectively increase the moisturizing and anti-wrinkle efficacy of the composition and play a good role in repairing skin cells. Detailed implementation manners

[0024] The present invention will be described below through specific examples to make the technical solutions of the present invention easier to understand and master, but the present invention is not limited thereto. The experimental methods described in the following examples are all conventional methods unless otherwise specified; the reagents and materials, unless otherwise specified, can all be obtained from commercial channels and are all standard products or analytical pure.

[0025] The sources of the components used herein are as follows:

[0026] Glucan, CAS: 9004-54-0, chemical formula: [C6H 10 O5]n, the molecular weight can be selected from 1 kDa to 2000 kDa, and the molecular weight used in the examples and comparative examples herein is 2000 kDa. β-Glucan, CAS: 9041-22-9, chemical formula: C 18 H 32 O 16 , molecular weight: 504.437.

[0027] The extracts in the composition of the present invention can all be made of raw materials available on the market. For comparison, the extracts used in this article are all obtained by reflux extraction with ethanol aqueous solution. Specifically as follows: Giant kelp extract: After the giant kelp is crushed, it is refluxed and extracted for 2 hours with a volume fraction of 75% ethanol aqueous solution, filtered and decolored after slag removal, and concentrated to obtain a giant kelp extract with an effective content of 90%. Centella asiatica extract: After the Centella asiatica is crushed, it is refluxed and extracted for 2 hours with a volume fraction of 75% ethanol aqueous solution, filtered and decolored after slag removal, and concentrated to obtain a Centella asiatica extract with an effective content of 90%. Aloe extract: After the aloe is crushed, it is refluxed and extracted for 2 hours with a volume fraction of 75% ethanol aqueous solution, filtered and decolored after slag removal, and concentrated to obtain an aloe extract with an effective content of 90%. Chamomile extract: After the chamomile is crushed, it is refluxed and extracted for 2 hours with a volume fraction of 75% ethanol aqueous solution, filtered and decolored after slag removal, and concentrated to obtain a chamomile extract with an effective content of 90%.

[0028] Example 1

[0029] A composition comprises the following components in parts by weight: 0.1 parts of glucan, 0.1 parts of beta-glucan, 0.1 parts of giant kelp extract, 10 parts of butanediol, 1 part of 1,2-hexanediol, and 60 parts of deionized water.

[0030] The preparation method of the composition comprises the following steps: adding glucan, β-glucan and giant kelp extract together into butanediol and 1,2-hexanediol for infiltration and dispersion, then adding them together into deionized water, heating to 50°C, stirring and dissolving at a rate of 100 rpm, keeping the temperature for 1 hour, cooling and filtering with a 300-mesh filter cloth at a temperature below 38°C to obtain the composition.

[0031] Example 2

[0032] A composition comprises the following components in parts by weight: 1 part of glucan, 1 part of beta-glucan, 0.5 part of giant kelp extract, 50 parts of butanediol, 5 parts of 1,2-hexanediol, and 120 parts of deionized water.

[0033] The preparation method of the composition comprises the following steps: adding glucan, β-glucan and giant kelp extract together into butanediol and 1,2-hexanediol for infiltration and dispersion, then adding them together into deionized water, heating to 90°C, stirring and dissolving at a rate of 30 rpm, keeping the temperature for 1 hour, cooling and filtering with a 300-mesh filter cloth at a temperature below 38°C to obtain the composition.

[0034] Example 3

[0035] A composition comprises the following components in parts by weight: 0.5 parts of glucan, 0.5 parts of beta-glucan, 0.2 parts of giant kelp extract, 20 parts of butanediol, 2 parts of 1,2-hexanediol, and 100 parts of deionized water.

[0036] The preparation method of the composition comprises the following steps: Dextran, β-glucan, and kelp extract are added together to 1,2-hexanediol and butylene glycol for infiltration and dispersion, and then added together to deionized water. Heat to 85 °C, stir and dissolve at a rate of 30 revolutions per minute, keep warm for 1 h, cool down, and filter with a 300-mesh filter cloth at a temperature below 38 °C to obtain the composition.

[0037] Example 4

[0038] A composition, by weight, comprises the following components: 0.5 parts of dextran, 0.5 parts of β-glucan, 0.1 part of kelp extract, 0.1 part of ectoin, 20 parts of butylene glycol, 2 parts of 1,2-hexanediol, and 100 parts of deionized water.

[0039] The preparation method of the composition comprises the following steps: Dextran, β-glucan, kelp extract, and ectoin are added together to 1,2-hexanediol and butylene glycol for infiltration and dispersion, and then added together to deionized water. Heat to 85 °C, stir and dissolve at a rate of 50 revolutions per minute, keep warm for 1 h, cool down, and filter with a 300-mesh filter cloth at a temperature below 38 °C to obtain the composition.

[0040] Comparative Example 1

[0041] Compared with Example 3, the difference is only that it does not contain dextran.

[0042] A composition, by weight, comprises the following components: 1 part of β-glucan, 0.2 part of kelp extract, 20 parts of butylene glycol, 2 parts of 1,2-hexanediol, and 100 parts of deionized water.

[0043] The preparation method of the composition comprises the following steps: β-glucan and kelp extract are added together to 1,2-hexanediol and butylene glycol for infiltration and dispersion, and then added together to deionized water. Heat to 85 °C, stir and dissolve at a rate of 30 revolutions per minute, keep warm for 1 h, cool down, and filter with a 300-mesh filter cloth at a temperature below 38 °C to obtain the composition.

[0044] Comparative Example 2

[0045] Compared with Example 3, the difference is only that it does not contain β-glucan

[0046] A composition, by weight, comprises the following components: 1 part of dextran, 0.2 part of kelp extract, 20 parts of butylene glycol, 2 parts of 1,2-hexanediol, and 100 parts of deionized water.

[0047] The preparation method of the composition comprises the following steps: adding glucan and giant kelp extract together into butanediol and 1,2-hexanediol for infiltration and dispersion, then adding them together into deionized water, heating to 85°C, stirring and dissolving at a rate of 30 rpm, keeping the temperature for 1 hour, cooling and filtering with a 300-mesh filter cloth at a temperature below 38°C to obtain the composition.

[0048] Comparative Example 3

[0049] Compared with Example 3, the only difference is that the macroalgae extract is not contained.

[0050] A composition comprises the following components in parts by weight: 0.6 parts of dextran, 0.6 parts of beta-glucan, 20 parts of butanediol, 2 parts of 1,2-hexanediol, and 100 parts of deionized water.

[0051] The preparation method of the composition comprises the following steps: adding glucan and β-glucan together into butanediol and 1,2-hexanediol for infiltration and dispersion, then adding them together into deionized water, heating to 85°C, stirring and dissolving at a rate of 30 rpm, keeping the temperature for 1 hour, cooling and filtering with a 300-mesh filter cloth at a temperature below 38°C to obtain the composition.

[0052] Comparative Example 4

[0053] Compared with Example 3, the only difference is that the giant kelp extract is replaced by the Centella asiatica extract.

[0054] A composition comprises the following components in parts by weight: 0.5 parts of glucan, 0.5 parts of beta-glucan, 0.2 parts of Centella asiatica extract, 20 parts of butylene glycol, 2 parts of 1,2-hexanediol, and 100 parts of deionized water.

[0055] The preparation method of the composition comprises the following steps: adding glucan, β-glucan and Centella asiatica extract together into butanediol and 1,2-hexanediol for infiltration and dispersion, then adding them together into deionized water, heating to 85°C, stirring and dissolving at a rate of 30 rpm, keeping the temperature for 1 hour, cooling and filtering with a 300-mesh filter cloth at a temperature below 38°C to obtain the composition.

[0056] Comparative Example 5

[0057] Compared with Example 3, the only difference is that the giant kelp extract is replaced by the aloe extract.

[0058] A composition comprises the following components in parts by weight: 0.5 parts of glucan, 0.5 parts of beta-glucan, 0.2 parts of aloe extract, 20 parts of butanediol, 2 parts of 1,2-hexanediol, and 100 parts of deionized water.

[0059] The preparation method of the composition comprises the following steps: adding glucan, β-glucan and aloe extract together into butanediol and 1,2-hexanediol for infiltration and dispersion, then adding them together into deionized water, heating to 85°C, stirring and dissolving at a rate of 30 rpm, keeping the temperature for 1 hour, cooling and filtering with a 300-mesh filter cloth at a temperature below 38°C to obtain the composition.

[0060] Comparative Example 6

[0061] Compared with Example 3, the only difference is that the giant kelp extract is replaced by the chamomile extract.

[0062] A composition comprises the following components in parts by weight: 0.5 parts of glucan, 0.5 parts of beta-glucan, 0.2 parts of chamomile extract, 20 parts of butylene glycol, 2 parts of 1,2-hexanediol, and 100 parts of deionized water.

[0063] The preparation method of the composition comprises the following steps: adding glucan, β-glucan and chamomile extract together into butanediol and 1,2-hexanediol for infiltration and dispersion, then adding them together into deionized water, heating to 85°C, stirring and dissolving at a rate of 30 rpm, keeping the temperature for 1 hour, cooling and filtering with a 300-mesh filter cloth at a temperature below 38°C to obtain the composition.

[0064] Effect test

[0065] 1. Moisturizing effect

[0066] Sample: Weigh the compositions of Examples 1-4, Comparative Examples 1-3 and Comparative Example 5, and add water to prepare a sample aqueous solution with an effective content of 5% (except for ionized water, the rest are effective ingredients);

[0067] Reference substance: Dissolve 0.5 ml of glycerol in 9.5 ml of aqueous solution and make up to 10 ml to prepare a 5% glycerol aqueous solution;

[0068] Test steps:

[0069] (1) Use a 1.5 ml EP tube as a carrier to weigh 1 g (tare weight) of 5% glycerol water, deionized water, and 5% sample aqueous solution (accurate to 0.0001 g);

[0070] (2) Place the samples, negative controls, and positive controls in an environment with a temperature of (22 ± 2)°C and a humidity of (50 ± 5)%, weigh them after 8 hours, and record the data;

[0071] (4) Calculate the moisture retention rate.

[0072] Moisture retention rate calculation formula: Moisture retention rate (%) = (M2) / (M1) × 100%;

[0073] Among them, M1 is the initial mass (g) of the sample to be tested, that is, 1 g; M2 is the mass (g) of the sample to be tested after being placed for 8 hours.

[0074] Table 1 Moisture Retention Rate of Samples

[0075] Experimental group M2 (g) Moisture retention rate % Control group 0.92 92 Example 1 0.95 95 Example 2 0.94 94 Example 3 0.95 95 Example 4 0.98 98 Comparative example 1 0.74 74 Comparative example 2 0.81 81 Comparative example 3 0.75 75 Comparative example 5 0.88 88

[0076] From the above data, it can be seen that Examples 1-4 of the present invention have obvious advantages in terms of moisture retention ability compared with Comparative Examples 1-3 and Comparative Example 5, and the composition provided by the present invention has better moisture retention and anti-wrinkle effects. Among them, according to the data of Example 3 and Comparative Examples 1-2, it can be known that the two glucans in the present invention have good synergistic effects. When both of them exist, the combination of the present invention can show more excellent moisture retention effects. According to the data of Comparative Examples 3 and 5, when an equal amount of glucan or β-glucan is used to replace the kelp extract or aloe extract with other moisture retention effects respectively, the moisture retention rate of the composition system is significantly reduced, indicating that the kelp extract and the glucan and β-glucan in the present invention have good synergistic effects.

[0077] As a preferred example of the present invention, in Example 4, the kelp extract was replaced with an equal amount of kelp extract and ectoine on the basis of Example 3, and the obtained composition system had better moisture retention effect, indicating that on the basis of Example 3, the kelp extract and ectoine in the preferred scheme of the present invention have better synergistic effects.

[0078] 2. Hyaluronidase Inhibition Test

[0079] Hyaluronidase is a participant in type I allergic reactions and has a strong correlation with inflammation and allergy. It is reported that some anti-allergic drugs have strong inhibitory effects on hyaluronidase activity. Therefore, inhibiting hyaluronidase activity can be used as an index for studying anti-allergic effects. Hyaluronidase is a glycoside hydrolase, and the end product of hydrolyzing sodium hyaluronate is N-acetyl-D-glucosamine. After the reaction, the unhydrolyzed sodium hyaluronate will be precipitated by the acidic BSA solution, so the inhibition rate of the sample on hyaluronidase can be reflected by detecting OD600nm.

[0080] Samples: Compositions of Examples 1-4 and Comparative Examples 1-4, Comparative Example 6, added with deionized water, and prepared into test samples with a concentration of 5 mg / mL;

[0081] VC reference substance: Vitamin C (VC), ≥99%; added with deionized water, and prepared into test samples with a concentration of 5 mg / mL.

[0082] Unless otherwise specified, all reagents used were of analytical grade, and the water was primary water meeting the requirements of GB / T6682, hyaluronidase (Solarbio, H8030), sodium hyaluronate (Solarbio, IS9010), vitamin C (VC) (Sigma, #SLCF5765), sodium acetate (Macklin, S817981), bovine serum albumin (BSA), sodium dihydrogen phosphate (Tianjin Damao Chemical Reagent Factory, 3486), disodium hydrogen phosphate (Tianjin Damao Chemical Reagent Factory, 3487), hydrochloric acid (Guangzhou Brand, zi082), sodium hydroxide (Tianjin Damao Chemical Reagent Factory, 3504):

[0083] (1) 10× Phosphate buffer: Weigh 3.56 g of Na2HPO4·2H2O and add deionized water to make up to 100 mL to obtain solution B; weigh 3.12 g of NaH2PO4·2H2O and add deionized water to make up to 100 mL to obtain solution A; mix 39 mL of solution A and 61 mL of solution B to get phosphate buffer A; weigh 4.51 g of NaCl, add it to phosphate buffer A and make up to 100 mL to obtain 10× phosphate buffer.

[0084] (2) 1% BSA: Weigh 0.1 g of BSA, add deionized water to make up to 10 mL, and store at 4°C.

[0085] (3) 1× Phosphate buffer: Take 1 mL of 10× phosphate buffer, add about 9 mL of deionized water and mix. Then add concentrated HCl and adjust the pH to 7.0 with a pH meter, and make up to 10 mL with deionized water.

[0086] (4) 0.01% BSA: Take 150 μL of 1% BSA, add 1.5 mL of 10× phosphate buffer, add about 12 mL of deionized water and mix. Then adjust the pH to 7.0 with a pH meter, and make up to 15 mL with deionized water.

[0087] (5) Weigh 15 mg of hyaluronidase, add it to 0.01% BSA and make up to 15 mL with a measuring cylinder to obtain a 1 mg / mL hyaluronidase solution. Dilute it 4 times before use, and the final concentration is 0.25 mg / mL (150 U / mL) hyaluronidase solution.

[0088] (6) 300 mM Phosphate buffer: Weigh 4.68 g of NaH2PO4·2H2O, add about 90 mL of deionized water, adjust the pH to 5.35 with NaOH, and make up to 100 mL.

[0089] (7) 0.5 mg / mL Sodium hyaluronate solution (HA): Weigh 3 mg of HA, add it to 5 mL of 300 mM phosphate buffer, stir to dissolve and then make up to 6 mL.

[0090] (8) 5 mg / mL BSA: Weigh 0.2955 g of sodium acetate and 0.75 g of BSA, add 130 mL of deionized water, add 675.5 μL of glacial acetic acid, adjust the pH to 3.75 with concentrated hydrochloric acid, and make up the volume to 150 mL.

[0091] Prepare the sample into a test solution with the corresponding concentration, prepare the reaction system according to the addition amounts and order of each reagent in Table 2, mix well, and react according to the corresponding reaction time and temperature.

[0092] Table 2 Reaction system for hyaluronidase inhibition test

[0093]

[0094] After the reaction is completed, use a microplate reader (Thermoscientific) to read the absorbance OD value at 600 nm, and calculate the inhibition rate of the sample on hyaluronidase according to the following formula.

[0095] Inhibition rate of the sample on hyaluronidase (%) = (C - D) / (A - B) × 100%;

[0096] In the formula:

[0097] A - Absorbance value of the blank group, containing sodium hyaluronate solution, without hyaluronidase solution and sample solution;

[0098] B - Background absorbance value of the blank group, containing sodium hyaluronate solvent, without hyaluronidase solution and sample solution;

[0099] C - Absorbance value of the sample group, containing sodium hyaluronate solution, hyaluronidase solution and sample solution;

[0100] D - Background absorbance value of the sample group, containing sodium hyaluronate solvent, hyaluronidase solution and sample solution.

[0101] Table 3 Results of hyaluronidase inhibition

[0102]

[0103]

[0104] From the above data, it can be seen that Examples 1-4 of the present invention have obvious advantages in anti-allergy ability compared with Comparative Examples 1-4 and 6, and the composition provided by the present invention has a better soothing and anti-allergy effect. Among them, according to the data of Example 3 and Comparative Examples 1-2, it can be known that the two glucans in the present invention have good synergistic effects. When both of them are present, the combination of the present invention can show a more excellent soothing and anti-allergy effect. According to the data of Comparative Examples 3-4 and 6, when an equal amount of glucan or β-glucan is used to replace the kelp extract respectively, or other centella asiatica extracts or chamomile extracts with soothing and anti-allergy effects are used, the hyaluronidase inhibition effect of the composition system is significantly reduced, indicating that the kelp extract and the glucan and β-glucan in the present invention have good synergistic effects.

[0105] As a preferred example of the present invention, in Example 4, the kelp extract was replaced with an equal amount of kelp extract and ectoin on the basis of Example 3, and the obtained composition system had a better soothing and anti-allergy effect, indicating that on the basis of Example 3, the kelp extract and ectoin in the preferred scheme of the present invention have better synergistic effects.

[0106] 3. Free radical (DPPH) scavenging experiment

[0107] DPPH free radicals have a single electron, so they have a very strong absorption near 517 nm and appear dark purple. When a free radical scavenger is present, the absorption gradually disappears due to the pairing of its single electron, and the degree of fading has a quantitative relationship with the number of electrons it receives. Therefore, the ability of the test sample to scavenge free radicals, that is, the size of the antioxidant activity, can be evaluated.

[0108] Samples: Compositions of Examples 1-4 and Comparative Examples 1-6;

[0109] VE reference substance: Vitamin E (VE), ≥98%;

[0110] Unless otherwise stated, all reagents used are of analytical grade, and the water is primary water conforming to GB / T6682, 95% ethanol (volume fraction, Tianjin Damao Chemical Reagent Factory, 20200801), DPPH (1,1-diphenyl-2-trinitrophenylhydrazine, TCI Company, WZ4DOME), vitamin E (Maclean, T834623):

[0111] (1) 0.12 mg / mL DPPH ethanol solution: Weigh 1,1-diphenyl-2-trinitrophenylhydrazine (DPPH), add 25 mL of 95% ethanol, and ultrasonically dissolve it for 5 min with an ultrasonic instrument to prepare a 0.12 mg / mL DPPH ethanol solution, and store it in a 4°C refrigerator in the dark.

[0112] (2) After diluting the compositions obtained in Examples 1-4 and Comparative Examples 1-6 with deionized water to prepare a composition solution of 0.05 mg / ml, 0.5 mL of each was mixed with 2.5 mL of DPPH solution to serve as the test groups, namely, the groups of Examples 1-4 and the groups of Comparative Examples 1-6; 0.5 mL of 95% ethanol was mixed with 2.5 mL of DPPH solution to serve as the blank group;

[0113] Vitamin E was completely dissolved in 95% ethanol to prepare a vitamin E solution of 0.05 mg / ml. Then, 0.5 mL of the vitamin E solution was mixed with 2.5 mL of DPPH solution to serve as the control group;

[0114] (3) After standing in the dark at room temperature for 30 min, the A value of each group was measured using a UV-visible spectrophotometer. 517 (The absorbance was measured using a 1-cm cuvette at a wavelength of 517 nm. The A value is the absorbance A value at a wavelength of 517 nm); Before starting the experiment, 2.5 mL of 95% ethanol was used to replace the DPPH solution for zero adjustment. After standing at room temperature for 20 min, the experiment was started. Each group of experiments was carried out in three parallel tests, and the average value was taken; Among them, 517 DPPH radical scavenging rate % = (A value of the blank group - A value of the experimental group) / A value of the blank group × 100%;

[0115] 517 517 517

[0116] The test results are shown in Table 4.

[0117] Table 4 DPPH radical scavenging rate %

[0118]

[0119]

[0120] As can be seen from the above data, Examples 1-4 of the present invention have obvious advantages in antioxidant ability compared with Comparative Examples 1-6, and the composition provided by the present invention has better antioxidant effects. Among them, according to the data of Example 3 and Comparative Examples 1-2, the two glucans in the present invention have good synergistic effects. When both of them are present, the composition of the present invention can exhibit more excellent antioxidant effects. According to the data of Comparative Examples 3-6, when an equal amount of glucan or β-glucan is used to replace the kelp extract separately, or other antioxidant extracts such as centella asiatica extract, aloe extract or chamomile extract are used, the effect of the composition system in scavenging DPPH radicals is significantly reduced. The kelp extract and the glucan and β-glucan in the present invention have good synergistic effects.

[0121] As a preferred example of the present invention, in Example 4, based on Example 3, the kelp extract was replaced with an equal amount of kelp extract and ectoine, and the antioxidant effect of the composition system was better, indicating that in the preferred solution of the present invention based on Example 3, the kelp extract and ectoine have a better synergistic effect.

[0122] The above detailed description is a specific description of one of the feasible embodiments of the present invention. This embodiment is not intended to limit the patent scope of the present invention. Any equivalent implementation or change without departing from the present invention should be included within the scope of the technical solution of the present invention.

Claims

1. A composition, comprising the following components: dextran, β - dextran, kelp extract, butylene glycol, 1,2 - hexanediol, deionized water.

2. The composition according to claim 1, characterized in that, By weight, it comprises the following components: 0.1 - 1 part of dextran, 0.1 - 1 part of β - dextran, 0.1 - 0.5 part of kelp extract, 10 - 50 parts of butylene glycol, 1 - 5 parts of 1,2 - hexanediol, and 60 - 120 parts of deionized water.

3. The composition according to claim 1, characterized in that, By weight, it comprises the following components: 0.1 - 0.5 part of dextran, 0.1 - 0.5 part of β - dextran, 0.2 - 0.5 part of kelp extract, 10 - 20 parts of butylene glycol, 1 - 2 parts of 1,2 - hexanediol, and 60 - 100 parts of deionized water.

4. The composition according to claim 1, characterized in that, The weight ratio of the dextran to the β - dextran is 0.1 - 1:1; preferably 1:

1.

5. The composition according to claim 1, wherein The weight ratio of the dextran, β - dextran to the kelp extract is 0.1 - 1:0.1 - 1:0.1 - 0.5; preferably 0.5:0.5:0.

2.

6. The composition according to claim 1, characterized in that, The composition may further contain ectoine, and the weight ratio of the ectoine to the kelp extract is 0.1:0.1 - 0.

5.

7. The preparation method of the composition according to claim 1, comprising the following steps: After infiltrating and dispersing dextran, β - dextran, and kelp extract together with butylene glycol and 1,2 - hexanediol, then adding them together into deionized water, heating, stirring until completely dissolved, cooling down to room temperature, and filtering to obtain the composition.

8. The preparation method of the composition according to claim 7, characterized in that, The heating temperature is 50 - 90 °C, preferably 85 °C.

9. The application of the composition according to any one of claims 2 - 7 in cosmetics or skin care products.

10. A cosmetic or skin care product, which comprises the composition according to any one of claims 2 - 7.

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