Application of rhizoma acori graminei polysaccharide in preparation of liver protection medicine

Abstract

The invention belongs to the technical field of biological medicines, and particularly relates to application of rhizoma acori graminei polysaccharide in preparation of a liver protection medicine. The rhizoma acori graminei polysaccharide is prepared by the following method: putting rhizoma acori graminei powder into a filter bag and soaking the rhizoma acori graminei powder in ethanol to obtain an alcohol extracting solution under the ultrasonic action; adding water into the alcohol extracting solution, carrying out heat extraction, and concentrating the obtained water extracting solution to obtain a crude polysaccharide extracting solution; adding a Sevage reagent into the polysaccharide crude extracting solution for deproteinization to obtain a polysaccharide extracting solution; and adding ethanol into the polysaccharide extracting solution for graded alcohol precipitation to obtain the rhizoma acori graminei polysaccharide. The rhizoma acori graminei polysaccharide disclosed by the invention can scavenge free radicals in vitro; in vivo, the D-gal galactose-induced liver aging injury can be effectively improved, the related antioxidant and inflammatory factor level can be regulated, and the D-gal galactose-induced liver aging injury has remarkable effects in liver protection and antioxidant and inflammatory aspects.

Description

Technical Field

[0001] The present invention belongs to the technical field of biomedicine, and particularly relates to the application of acorus tatarinowii polysaccharide in the preparation of liver protection drugs. Background Art

[0002] The liver undertakes core physiological functions such as material metabolism regulation, degradation of endogenous and exogenous toxins, and immune homeostasis regulation, and is a key hub for maintaining the stable operation of the body. However, liver function shows a progressive decline with aging, and is extremely prone to senescent damage due to mechanisms such as oxidative stress, inflammatory response, and lipid metabolism disorder. There is an urgent need for safe and effective drugs to protect against liver aging-induced damage.

[0003] Currently, drugs with liver protection effects mainly include chemically synthesized drugs and natural product extracts. Chemically synthesized drugs include the antioxidant N-acetylcysteine, the anti-inflammatory drug ursodeoxycholic acid, etc.; natural product extracts include silymarin, glycyrrhizin preparations, curcumin, etc. Although these drugs have certain effects in improving liver function indicators and reducing liver injury, they generally have problems such as a single action target, the risk of side effects with long-term use, or low bioavailability, which limit their wide application. Therefore, the development of natural drugs with multiple targets and multiple mechanisms has become a research hotspot.

[0004] Acorus tatarinowii is a traditional Chinese medicine. Its nature and flavor are pungent and warm, and it belongs to the heart, liver, and spleen meridians. It has the functions of opening orifices and resolving phlegm, and regulating qi and stomach with dampness. It is commonly used to treat symptoms such as coma and damp turbidity blocking the middle energizer. The main active ingredients of acorus tatarinowii include acorus tatarinowii volatile oil and acorus tatarinowii polysaccharide components. A large number of studies have shown that acorus tatarinowii volatile oil has a significant protective effect on the nervous system. Among them, α-asarone and β-asarone in acorus tatarinowii volatile oil exhibit various pharmacological activities such as sedation, anti-convulsion, and improvement of learning and memory. They not only have great potential in the prevention and treatment of nervous system diseases, but also play a certain role in the treatment of alcoholic liver toxicity. Acorus tatarinowii polysaccharide has potential activities such as antioxidant and immune regulation, and has also been found to have the effect of treating neurotoxicity and neuroinflammation.

[0005] Currently, the research on acorus tatarinowii mainly focuses on the active functions of acorus tatarinowii volatile oil, and there are still significant limitations in the research on acorus tatarinowii polysaccharide. Summary of the Invention

[0006] To solve the above technical problems, the present invention provides the application of acorus tatarinowii polysaccharide in the preparation of liver protection drugs. The acorus tatarinowii polysaccharide extracted by the present invention has the effect of resisting liver oxidative stress and inflammation, reveals the action mechanism and potential value of acorus tatarinowii polysaccharide in organisms, and provides a theoretical basis for its application in the fields of liver protection and anti-aging. Acorus tatarinowii polysaccharide shows great potential application prospects in the research and development field of anti-aging drugs and is expected to become a key active ingredient. Acorus tatarinowii polysaccharide has clear medicinal value in liver protection and provides a new natural drug option for the prevention and treatment of liver-related diseases.

[0007] The purpose of the present invention is to provide the application of acorus tatarinowii polysaccharide in the preparation of liver protection drugs.

[0008] The liver protection drug is a drug for improving liver aging injury induced by D-galactose or a drug for reducing liver injury caused by oxidative stress.

[0009] The present invention uses acorus tatarinowii as the raw material, and prepares acorus tatarinowii polysaccharide through processes of filtration, separation of protein, and fractional alcohol precipitation.

[0010] The acorus tatarinowii polysaccharide described in the present invention is obtained by the following method:

[0011] Soak the acorus tatarinowii powder in ethanol, and under the action of ultrasound, remove the fat-soluble substances and pigments in the acorus tatarinowii to obtain an alcohol extract.

[0012] Add water to the alcohol extract for hot extraction, and concentrate the obtained hot extraction solution to obtain a crude polysaccharide extract.

[0013] Add Sevage reagent to the crude polysaccharide extract for protein removal to obtain a polysaccharide extract.

[0014] Add ethanol to the polysaccharide extract for fractional alcohol precipitation to obtain acorus tatarinowii polysaccharide.

[0015] It should be noted that in the present invention, through ultrasonic-assisted filtration, firstly, direct physical contact with the acorus tatarinowii powder is avoided; secondly, through ultrasound, not only can fat-soluble substances and pigments be efficiently removed, but also the low loss of effective polysaccharide components can be maximally guaranteed, the yield can be increased, and the cost can be reduced.

[0016] Preferably, the time of the ultrasound is 30 min to 40 min, and the temperature is room temperature.

[0017] Preferably, before soaking the acorus tatarinowii powder, place the acorus tatarinowii powder in a filter bag, and the pore size of the filter bag is 50 μm to 75 μm.

[0018] Preferably, the dosage ratio of the acorus tatarinowii powder to ethanol is 1 g: 10 ml to 15 ml.

[0019] Preferably, the dosage ratio of the Acorus tatarinowii Schott powder to water is 1 g: 10 ml to 15 ml.

[0020] Preferably, the temperature of the hot extraction is 95°C to 100°C.

[0021] Preferably, the Sevage reagent is prepared by mixing chloroform and n-butanol in a volume ratio of 4:1.

[0022] Preferably, the volume fraction of ethanol in the fractional alcohol precipitation is 95%.

[0023] Preferably, the Acorus tatarinowii Schott powder is obtained by crushing the medicinal parts of Acorus tatarinowii Schott, namely the roots or rhizomes.

[0024] Compared with the prior art, the present invention has the following beneficial effects:

[0025] The Acorus tatarinowii Schott polysaccharide of the present invention has a liver protection effect, which is mainly manifested in its ability to slow down liver senescence damage caused by oxidative stress and inflammation, and is used for preventing and treating liver senescence damage. It shows that the Acorus tatarinowii Schott polysaccharide has the potential to become a high-quality natural liver protection medicinal material, providing a strong experimental basis for subsequent related research and application development.

[0026] The present invention uses Acorus tatarinowii Schott as the raw material, and obtains Acorus tatarinowii Schott polysaccharide through processes such as filtration, protein separation, and fractional alcohol precipitation. During the filtration and purification process, through ultrasonic-assisted filtration, while removing fat-soluble substances and pigments, it can also ensure the low loss of the effective components of the polysaccharide to the greatest extent and increase the yield. At the same time, the present invention separates Acorus tatarinowii Schott polysaccharides with different molecular weights through fractional alcohol precipitation, and the preparation method is simple, the yield is high, and the product is pure. Description of the Drawings

[0027] Figure 1 Shows the changes in liver tissues of the control group, model group, AT40 group, and AT40-AT80 group; among them, a is the control group, b is the model group, c is the AT40-AT80 group, and d is the AT40 group.

[0028] Figure 2 Shows the immunohistochemical results of TLR4, NRF2, and HO-1; among them, a is TLR4, b is NRF2, and c is HO-1; the 1 in the abscissa represents the blank group, 2 represents the model group, 3 represents the AT40-AT80 group, and 4 represents the AT40 group.

[0029] Figure 3 Shows the scavenging effect of different concentrations of AT40, AT80, and ascorbic acid on ABTS free radicals. Detailed Embodiments

[0030] It should be noted that many polysaccharides play a key role in antioxidant stress, especially in the liver, the largest metabolic organ in the human body, where the efficacy of polysaccharides is more significant. For example, polysaccharides from Echinacea plants can effectively resist liver damage induced by oxidative stress; purple sweet potato polysaccharides can reduce the degree of liver damage by inhibiting inflammation and oxidative stress; oyster polysaccharides also show positive effects in resisting liver oxidative stress and inflammation. Thus, it can be seen that the polysaccharide components of many Chinese herbal medicines have significant effects in combating liver oxidative stress and inflammation.

[0031] In addition, as the active ingredient asarone in Acorus tatarinowii not only has an anti-neuroinflammatory effect but also has a protective effect on the liver, and it has also been found that there are components in other Chinese herbal medicine polysaccharides that can exert antioxidant stress effects in the liver.

[0032] Based on this, the present invention explores the effect of Acorus tatarinowii polysaccharide components in resisting liver oxidative stress and inflammation, and delays aging through this approach.

[0033] The present invention uses Acorus tatarinowii as the raw material, and prepares Acorus tatarinowii polysaccharide through processes such as filtration, protein separation, and fractional alcohol precipitation.

[0034] The Acorus tatarinowii polysaccharide described in the present invention is obtained by the following method:

[0035] Filtration and purification: Soak the Acorus tatarinowii powder in ethanol, and under the action of ultrasound, remove the fat-soluble substances and pigments in Acorus tatarinowii to obtain an alcohol extract.

[0036] Hot water extraction: Add water to the alcohol extract for hot extraction, and concentrate the obtained hot extract to obtain a crude polysaccharide extract.

[0037] Protein separation: Add Sevage reagent to the crude polysaccharide extract for protein removal to obtain a polysaccharide extract.

[0038] Fractional alcohol precipitation: Add ethanol to the polysaccharide extract for fractional alcohol precipitation to obtain Acorus tatarinowii polysaccharide.

[0039] It should be noted that the present invention uses ethanol with different concentrations to extract Acorus tatarinowii polysaccharides with different molecular weights, which is based on the molecular weight and polarity characteristics of polysaccharides. Generally, the Acorus tatarinowii polysaccharide precipitated by ethanol with a volume fraction of 40% has a larger molecular weight and contains more hydrophobic components. As the ethanol concentration increases, the molecular weight of the precipitated Acorus tatarinowii polysaccharide will gradually decrease.

[0040] In the process of fractional alcohol precipitation of the present invention, the content of Acorus tatarinowii polysaccharide extracted with ethanol with a volume fraction of 40% is 3.79%; the content of Acorus tatarinowii polysaccharide extracted with ethanol with a volume fraction of 80% is 2.67%.

[0041] As the ethanol concentration increases, the content of the extracted Acorus tatarinowii polysaccharide gradually decreases; in the present invention, ethanol with a volume fraction of 90% is not used for further extraction because the molecular weight of the extracted polysaccharide is too small and the content of the extracted polysaccharide is too low, consuming manpower and material resources.

[0042] The full English name of nuclear factor erythroid 2-related factor 2 is nuclear factor erythroid 2-related factor 2, and its English abbreviation is NRF2; the full English name of Toll-like receptor 4 is Toll-like receptor 4, and its English abbreviation is TLR4; the full English name of heme oxygenase-1 is heme oxygenase 1, and its English abbreviation is HO-1; the full English name of 2,2′-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) is 2,2'-Azino-bis(3-ethylbenzothiazoline-6-sulfonic acid), and its English abbreviation is ABTS.

[0043] To enable those skilled in the art to better understand and implement the technical solution of the present invention, the present invention will be further described below in conjunction with specific embodiments and the accompanying drawings.

[0044] In the description of the present invention, unless otherwise specified, the reagents used are commercially available, and the methods used are conventional techniques in the art.

[0045] Example 1

[0046] This example provides a method for preparing Acorus tatarinowii polysaccharide.

[0047] Step 1, filtration and purification:

[0048] Wash the Acorus tatarinowii, remove the mud, dry and crush it to obtain Acorus tatarinowii powder.

[0049] Take 200 g of Acorus tatarinowii powder and place it in a filter bag with a pore size of 50 μm to 75 μm.

[0050] Immerse the filter bag containing Acorus tatarinowii powder in 2 L of absolute ethanol and perform ultrasonic filtration for 30 min.

[0051] Replace the absolute ethanol and repeat the above operation 3 times.

[0052] Wash the filter bag containing Acorus tatarinowii powder after 3 times of ultrasonic treatment with water to remove ethanol.

[0053] Step 2, hot water extraction:

[0054] Put the filter bag containing Acorus tatarinowii powder after water washing into a rice cooker, add 2 L of distilled water, and heat for 3 h.

[0055] Replace the distilled water and repeat the previous operation 3 times.

[0056] Concentrate the obtained aqueous extract under reduced pressure to 400 ml to obtain a crude polysaccharide extract.

[0057] Step 3: Protein separation:

[0058] Add 100 ml of Sevage reagent to the crude polysaccharide extract to obtain a mixture; among them, the Sevage reagent is composed of chloroform and n-butanol mixed at a volume ratio of 4:1.

[0059] Transfer the mixture into a separating funnel, shake it well for 10 min, centrifuge it at a high speed of 4000 rpm for 20 min, discard the middle layer of protein and the lower layer of organic phase, and collect the upper aqueous phase.

[0060] Add Sevage reagent to the upper aqueous phase and repeat the previous operation 3 times to obtain a polysaccharide extract.

[0061] Step 4: Fractional alcohol precipitation:

[0062] Under magnetic stirring, slowly add ethanol with a volume fraction of 95% to the polysaccharide extract until the ethanol concentration in the polysaccharide extract reaches 40%, immerse it overnight, centrifuge it at a high speed of 4000 rpm for 15 min the next day, and then filter it to collect the first filtrate and the first filter residue; dry and grind the first filter residue to obtain powdery Acorus tatarinowii crude polysaccharide, denoted as AT40.

[0063] Perform rotary evaporation on the first filtrate to obtain a first mixture. Under magnetic stirring, slowly add ethanol with a volume fraction of 95% to the first mixture until the ethanol concentration in the polysaccharide extract reaches 60%, immerse it overnight, centrifuge it at a high speed of 4000 rpm for 15 min the next day, and then filter it to collect the second filtrate and the second filter residue; dry and grind the second filter residue to obtain powdery Acorus tatarinowii crude polysaccharide, denoted as AT60.

[0064] Perform rotary evaporation on the second filtrate to obtain a second mixture. Under magnetic stirring, slowly add ethanol with a volume fraction of 95% to the second mixture until the ethanol concentration in the polysaccharide extract reaches 80%, immerse it overnight, centrifuge it at a high speed of 4000 rpm for 15 min the next day, and then filter it to collect the third filter residue; dry and grind the third filter residue to obtain powdery Acorus tatarinowii crude polysaccharide, denoted as AT80.

[0065] I. In vivo experiments.

[0066] The crude Acorus tatarinowii polysaccharide (AT40) prepared in Example 1 was used as the raw material, and water was used as the solvent to prepare an AT40 solution with a mass fraction of 5%. The crude Acorus tatarinowii polysaccharide (AT40) and the crude Acorus tatarinowii polysaccharide (AT80) prepared in Example 1 were mixed at a mass ratio of 1:1, and water was used as the solvent to prepare a mixed solution of AT40-AT80 with a mass fraction of 5%.

[0067] In this experiment, SD rats were used. Before the experiment, they were randomly divided into four groups: a blank group, a model group, an AT40 group, and an AT40-AT80 group. The specific operations were as follows: Blank group: An appropriate amount of normal saline was administered continuously for eight weeks. Model group: D-galactose was subcutaneously injected continuously for eight weeks at a dose of 1000 mg / kg once a day to construct an aging model. AT40 group: D-galactose was subcutaneously injected continuously for eight weeks at a dose of 1000 mg / kg once a day; and starting from the third week, the AT40 solution was administered orally at a dose of 200 mg / kg for six consecutive weeks. AT40-AT80 group: D-galactose was subcutaneously injected continuously for eight weeks at a dose of 1000 mg / kg once a day; starting from the third week, the mixed solution of AT40-AT80 was administered orally at a dose of 200 mg / kg for six consecutive weeks. During the modeling period, the body weight, food intake, and water intake of the rats were accurately measured and recorded at regular intervals every day to ensure that the vital signs of the rats were normal and their growth status was good.

[0068] After the modeling was completed, the liver tissues of the rats were completely removed and subjected to conventional fixation, dehydration, embedding, etc. Subsequently, HE staining and immunohistochemical analysis were performed, and the pathological changes of the liver tissues were observed under a microscope to provide an intuitive morphological basis for subsequent analysis.

[0069] 1. Staining analysis of the pathological tissues of the rat liver.

[0070] For this invention, the liver tissues of the rats were fixed with 4% paraformaldehyde by mass for 24 h and embedded in paraffin for sectioning. HE staining was used to observe the changes in the liver tissues under a microscope. The results are as Figure 1 .

[0071] As Figure 1 shown in a of, the liver tissue structure of the rats in the control group was clear, the hepatocyte morphology was regular, the sizes were uniform, and the hepatic cords were neatly arranged radially around the central vein. As Figure 1 shown in b of, the liver structure of the rats in the model group was significantly damaged, manifested as an increase in the number of binuclear hepatocytes, loose arrangement of hepatocytes, a significant increase in the cell gap, and at the same time, there were certain degrees of ballooning degeneration and inflammatory cell infiltration. As Figure 1As shown in c and d, after treatment with Acorus tatarinowii polysaccharide, the liver condition of rats in the AT40 group and the AT40-AT80 group was significantly improved, and the hepatocyte morphology was clear, arranged neatly, approaching the control group level.

[0072] 2. Immunohistochemical analysis of the anti-aging level of rat liver.

[0073] Through the immunohistochemical results, the present invention analyzed the changes of three indexes, NRF2, TLR4, and HO-1, and explored the protective effect of Acorus tatarinowii polysaccharide on the liver. The results are as Figure 2 .

[0074] As can be seen from Figure 2 , after treatment with D-galactose, the content of NRF2 in rats decreased significantly, indicating a decrease in the body's antioxidant capacity. After treatment with Acorus tatarinowii polysaccharide, the content of NRF2 increased; the increase amplitude in the AT40-AT80 group was more obvious. In addition, the contents of TLR4 and HO-1 in the model group both increased significantly. The increase in TLR4 aggravated the level of oxidative stress, and the increase in HO-1 in macrophages triggered an inflammatory response. After treatment with Acorus tatarinowii polysaccharide, the contents of TLR4 and HO-1 both decreased, and the AT40-AT80 group and the AT40 group had similar effects in reducing the contents of these two factors, alleviating oxidative stress and inflammatory responses.

[0075] The in vivo experimental results of the present invention show that Acorus tatarinowii polysaccharide can effectively improve D-galactose-induced liver senescence injury, regulate the levels of related antioxidant and inflammatory factors, and show significant effects in liver protection, antioxidant and anti-inflammatory aspects. This indicates that Acorus tatarinowii polysaccharide has the potential to become a high-quality natural liver protection medicinal material, providing a strong experimental basis for subsequent related research and application development.

[0076] II. In vitro experiment.

[0077] The present invention uses water as a solvent to prepare a potassium persulfate solution with a concentration of 1.4 mmol / L and an ABTS solution with a concentration of 7 mmol / L; 10 mL of the potassium persulfate solution and 10 mL of the ABTS solution are respectively measured and fully mixed, and reacted in the dark at room temperature for 16 h to obtain an ABTS radical solution. Before use, the absorbance of the ABTS radical solution is measured at OD734nm, and the ABTS radical solution is diluted to an absorbance of 0.7 ± 0.02.

[0078] In this invention, water is used as a solvent, and the AT40 Acorus tatarinowii polysaccharide prepared in Example 1 is successively formulated into AT40 solutions with concentrations of 2 mg / mL, 4 mg / mL, 6 mg / mL, 8 mg / mL, and 10 mg / mL to obtain AT40 solutions with different concentrations. 20 μL of the AT40 solutions with different concentrations and the diluted ABTS radical solution are added into a 96-well microplate. After shaking for 10 s and mixing evenly, the mixture is allowed to stand and react at 37 °C for 5 min to obtain five sample group solutions with different concentrations, denoted as the sample group. 20 μL of double-distilled water is measured and added into the diluted ABTS radical solution. After mixing evenly, the mixture is allowed to stand and react at 37 °C for 5 min to obtain a blank group solution, denoted as the blank group. 20 μL of the pigment solution is measured and added into 180 μL of distilled water. After mixing evenly, the mixture is allowed to stand and react at 37 °C for 5 min to obtain a control group solution, denoted as the control group. Ascorbic acid is used as a positive control, and the concentration of ascorbic acid is the same as that of the AT40 solution. The absorbances of the sample group, the blank group, and the control group at OD734 nm are measured respectively. Four parallel experiments are carried out for each group, and the average value is taken. The ABTS scavenging rate is calculated according to the following formula:

[0079] ABTS scavenging rate % = [1 - (A1 - A2) / A0] × 100%;

[0080] Among them, A1 is the absorbance value of the sample group, A0 is the absorbance value of the blank group, and A2 is the absorbance value of the control group.

[0081] In this invention, the AT80 Acorus tatarinowii polysaccharide prepared in Example 1 is formulated into AT80 solutions with concentrations of 2 mg / mL, 4 mg / mL, 6 mg / mL, 8 mg / mL, and 10 mg / mL to obtain AT80 solutions with different concentrations; then the AT80 solutions with different concentrations are treated in the same way to obtain the sample group. The absorbances of the sample group, the blank group, and the control group at OD734 nm are measured respectively. Four parallel experiments are carried out for each group, and the average value is taken to further calculate the ABTS scavenging rate of AT80.

[0082] It can be Figure 3 seen that ascorbic acid and Acorus tatarinowii polysaccharide have different degrees of scavenging effects on ABTS; the ABTS scavenging rates of AT80 and AT40 increase with the increase of concentration, but the scavenging effects of both are lower than that of ascorbic acid. When the mass concentration is 10 mg / mL, the ABTS scavenging rate of AT80 is about 60%, while the scavenging rate of AT40 is about 50%. This shows that the Acorus tatarinowii polysaccharide prepared in this invention all shows a certain free radical scavenging ability and can reduce the damage of oxidative stress to the liver.

[0083] It should be noted that when the present invention involves a numerical range, it should be understood that both endpoints of each numerical range and any value between the two endpoints can be selected. Since the steps and methods adopted are the same as those in the embodiments, in order to avoid redundancy, the present invention describes the preferred embodiments. Although the preferred embodiments of the present invention have been described, once those skilled in the art learn the basic creative concepts, additional changes and modifications can be made to these embodiments, and these changes and modifications all fall within the scope of the present invention.

[0084] Obviously, those skilled in the art can make various changes and modifications to the present invention without departing from the spirit and scope of the present invention. If these modifications and variations of the present invention fall within the scope of the equivalent technology of the present invention, the present invention also intends to include these changes and modifications.

Claims

1. Application of acorus tatarinowii polysaccharide in preparing liver protection drugs.

2. Use of the acorus tatarinowii polysaccharide according to claim 1 in the preparation of a liver protection drug, characterized in that, The liver protection drugs are drugs for improving liver senescence injury induced by D-galactose or drugs for reducing liver injury caused by oxidative stress.

3. Use of acorus tatarinowii polysaccharide according to claim 1 in the preparation of a liver protection drug, characterized in that, The acorus tatarinowii polysaccharide is prepared by the following method: Soak the acorus tatarinowii powder in ethanol, and under the action of ultrasound, remove the fat-soluble substances and pigments in the acorus tatarinowii to obtain an ethanol extract; Add water to the ethanol extract for hot extraction, and concentrate the obtained hot extract to obtain a crude polysaccharide extract; Add Sevage reagent to the crude polysaccharide extract for deproteinization to obtain a polysaccharide extract; Add ethanol to the polysaccharide extract for fractional ethanol precipitation to obtain acorus tatarinowii polysaccharide.

4. Use of the acorus tatarinowii polysaccharide according to claim 3 in the preparation of a liver protection drug, characterized in that, The time of the ultrasound is 30 min to 40 min, and the temperature is room temperature.

5. Use of the acorus tatarinowii polysaccharide according to claim 3 in the preparation of a liver protection drug, characterized in that, The temperature of the hot extraction is 95 °C to 100 °C.

6. Use of the acorus tatarinowii polysaccharide according to claim 3 in the preparation of a liver protection drug, characterized in that, The dosage ratio of the acorus tatarinowii powder to ethanol is 1 g: 10 ml to 15 ml; The dosage ratio of the acorus tatarinowii powder to water is 1 g: 10 ml to 15 ml.

7. Use of the acorus tatarinowii polysaccharide according to claim 3 in the preparation of a liver protection drug, characterized in that, The volume fraction of ethanol in the fractional ethanol precipitation is 95%.

8. Use of the acorus tatarinowii polysaccharide according to claim 3 in the preparation of a liver protection drug, characterized in that, The acorus tatarinowii powder is obtained by crushing the medicinal parts of acorus tatarinowii, namely roots or rhizomes.

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