Fermentation product with blood fat reducing effect and preparation method thereof

Abstract

The invention belongs to the technical field of traditional Chinese medicine fermentation, and particularly relates to a fermentation product with a blood fat reducing effect and a preparation method. The fermentation product is prepared from the following raw materials in parts by weight: 20 to 50 parts of fructus crataegi, 20 to 40 parts of folium nelumbinis, 10 to 30 parts of exocarpium citri grandis, 30 to 90 parts of rhizoma curcumae longae, 5 to 25 parts of celery seeds, 8 to 18 parts of an enzyme preparation and 0.04 to 0.24 part of a leavening agent. The fermentation product obtained from the medicinal and edible raw materials through enzymolysis and fermentation technologies can reduce the content level of total cholesterol (TC), triglyceride (TG) and low-density lipoprotein cholesterol (LDL-C) in serum and improve the level of high-density lipoprotein cholesterol (HDL-C), and has a remarkable blood fat reducing effect.

Description

Technical Field

[0001] The present invention belongs to the technical field of traditional Chinese medicine fermentation, and particularly relates to a fermentation product with lipid-lowering efficacy and a preparation method thereof. Background Art

[0002] Dyslipidemia refers to one or more lipid substances such as cholesterol, triglyceride, phospholipid, and non-free fatty acid in plasma exceeding the normal range, which is mostly caused by abnormal fat metabolism and transport. Dyslipidemia not only causes cardiovascular and cerebrovascular diseases such as atherosclerosis, coronary heart disease, and stroke, but also damages organs such as the liver and kidneys, seriously threatening human health.

[0003] Currently, although the drugs used clinically for treating dyslipidemia have certain curative effects, most of them have side effects, such as causing liver function damage and gastrointestinal discomfort, and drug resistance will occur after long-term use.

[0004] Traditional Chinese medicine can not only reduce the burden on the body functions of patients, especially the burden on the liver and kidneys, but also has higher safety. This means that traditional Chinese medicine can avoid the adverse reactions brought by western medicine. For example, Chinese Patent CN106668326A discloses a traditional Chinese medicine composition for treating hyperlipidemia and a preparation method thereof, which includes: 10-15 parts by weight of Exocarpium Citri Grandis, 10-15 parts by weight of Cortex Dictamni, 7-9 parts by weight of Folium Nelumbinis, 7-9 parts by weight of Codonopsis Pilosula, 7-9 parts by weight of Pueraria Lobata, 7-9 parts by weight of Cuscuta Chinensis, 7-9 parts by weight of Lycium Barbarum, 7-9 parts by weight of Fructus Crataegi, and 7-9 parts by weight of Aucklandia Lappa. The above takes Exocarpium Citri Grandis and Cortex Dictamni as the main raw materials, and the Cortex Dictamni and Aucklandia Lappa used are not raw materials that are both medicine and food, so the scope of use is limited. Summary of the Invention

[0005] For this reason, on the one hand, the present invention provides a fermentation product with lipid-lowering efficacy, which includes five raw materials that are both medicine and food, namely Exocarpium Citri Grandis, Folium Nelumbinis, Fructus Crataegi, Curcuma Longa, and celery seeds, and is prepared by enzymatic hydrolysis and fermentation. The fermentation product can significantly reduce the levels of serum total cholesterol (TC), triglyceride (TG), and low-density lipoprotein cholesterol (LDL-C), and increase the level of high-density lipoprotein cholesterol (HDL-C), thus playing a lipid-lowering role.

[0006] On the other hand, a method for preparing the above fermentation product is provided, and this method is simple and can be used for industrial production.

[0007] To achieve the above object, the present invention provides a lipid-lowering fermentation product, including Fructus Crataegi, Folium Nelumbinis, Exocarpium Citri Grandis, Curcuma Longa, celery seeds, enzyme preparation, and fermenting agent.

[0008] Preferably, the fermentation product for reducing blood lipid provided by the present invention, by weight, comprises 20-50 parts of hawthorn, 20-40 parts of lotus leaf, 10-30 parts of tangerine peel, 30-90 parts of turmeric, 5-25 parts of celery seed, 8-18 parts of enzyme preparation and 0.04-0.24 parts of ferment.

[0009] Preferably, the ferment, by weight, comprises 0.02-0.12 parts of Lactobacillus rhamnosus, 0.01-0.07 parts of Lactobacillus acidophilus and 0.01-0.05 parts of Lactobacillus casei.

[0010] More preferably, the weight ratio of Lactobacillus rhamnosus, Lactobacillus acidophilus and Lactobacillus casei is (4-10):(3-5):(2-4).

[0011] Even more preferably, the weight ratio of Lactobacillus rhamnosus, Lactobacillus acidophilus and Lactobacillus casei is 7:4:3.

[0012] Preferably, the viable count of Lactobacillus rhamnosus is 6000-8000 billion CFU / g, the viable count of Lactobacillus acidophilus is 100-5000 billion CFU / g, and the viable count of Lactobacillus casei is 4000-6000 billion CFU / g.

[0013] Preferably, the enzyme preparation comprises cellulose and acid protease.

[0014] More preferably, the enzyme preparation, by weight, comprises 5-10 parts of cellulase and 3-8 parts of acid protease.

[0015] Even more preferably, the weight ratio of cellulase and acid protease is 7:5.

[0016] Preferably, the enzyme activity of cellulase is 20000-40000 U / g, and the enzyme activity of acid protease is 50000-100000 U / g.

[0017] Preferably, the weight ratio of hawthorn, lotus leaf, tangerine peel, turmeric and celery seed is (30-40):(25-35):(15-25):(50-70):(10-20).

[0018] More preferably, the weight ratio of turmeric and tangerine peel is (2-3.5):1; even more preferably, the weight ratio of turmeric and tangerine peel is 3:1.

[0019] More preferably, the weight ratio of turmeric and lotus leaf is (0.75-4.5):1; even more preferably, the weight ratio of turmeric and lotus leaf is 2:1.

[0020] Further preferably, the weight ratio of turmeric to celery seeds is (3.5 - 6):1; even more preferably, the weight ratio of turmeric to celery seeds is 4:1.

[0021] Even more preferably, the weight ratio of hawthorn, lotus leaf, tangerine peel, turmeric and celery seeds is 35:30:20:60:15.

[0022] The present invention also provides a method for preparing a fermentation product with lipid-lowering efficacy, comprising the following steps:

[0023] (1) Crushing hawthorn, lotus leaf, tangerine peel, turmeric and celery seeds to obtain micronized powder, and mixing the micronized powder with water to form a traditional Chinese medicine mixture;

[0024] (2) Adjusting the pH of the traditional Chinese medicine mixture, and then adding an enzyme preparation for enzymatic hydrolysis to obtain an enzymatic hydrolysate;

[0025] (3) Heating the enzymatic hydrolysate to inactivate the enzyme and extract, to obtain an enzyme-inactivated extract;

[0026] (4) Adding a fermenting agent to the enzyme-inactivated extract for fermentation to obtain a fermentation broth; performing solid-liquid separation on the fermentation broth to obtain a fermentation clear liquid, which is the fermentation product.

[0027] Preferably, the conditions for crushing in step (1) are: crushing temperature 20 - 40°C, crushing pressure 10 - 15 Mpa, air flow rate 300 - 400 m / s, and crushing time 1 - 2 h.

[0028] Preferably, the temperature for enzymatic hydrolysis in step (2) is 40 - 65°C, and the enzymatic hydrolysis time is 50 - 100 min.

[0029] Even more preferably, in step (2), the pH is adjusted to 5.0 - 6.0.

[0030] Preferably, the temperature for enzyme-inactivated extraction in step (3) is 80 - 100°C, and the enzyme-inactivated extraction time is 40 - 80 min.

[0031] Preferably, the fermentation temperature in step (4) is 20 - 40°C, and the fermentation time is 16 - 32 h.

[0032] Use of the above fermentation product in the preparation of a lipid-lowering product.

[0033] Compared with the prior art, the present invention has the following advantages:

[0034] 1. The lipid-lowering fermentation product provided by the present invention uses five raw materials, namely hawthorn, lotus leaf, tangerine peel, turmeric, and celery seeds, as the main components. Among them, hawthorn is sour, sweet, and slightly warm in nature, and belongs to the spleen, stomach, and liver meridians. It has the effects of strengthening the stomach and promoting digestion, dispersing stasis and promoting qi movement, and reducing turbidity and lipid. The flavonoids, triterpenoids, etc. contained in it can reduce blood lipid and dilate blood vessels. Lotus leaf is bitter in taste, neutral in nature, and belongs to the liver, spleen, and stomach meridians. It has the effects of clearing summer heat and promoting dampness, ascending the clear yang, cooling blood and stopping bleeding, and clearing the heart and removing heat. Lotus leaf contains various bioactive components such as nuciferine and flavonoids. Among them, nuciferine can regulate fat metabolism, inhibit the activity of lipase, and reduce the absorption of fat. Tangerine peel is warm in nature, bitter, slightly pungent, and aromatic, and belongs to the lung and spleen meridians. Its main active substances are volatile oils, flavonoid compounds, and alkaloids. Among them, alkaloids have the effect of reducing lipid and losing weight, and naringin has a protective effect on the cardiovascular system. Turmeric is pungent, bitter, and warm in nature, and belongs to the spleen and liver meridians. The main active ingredient of turmeric is curcumin, and curcumin has the effect of reducing blood lipid. Celery seeds are cool in nature, slightly bitter in taste, and enter the lung meridian, liver meridian, and stomach meridian. Its main active substances are volatile oils and flavonoids. Among them, the flavonoid extract has the effect of reducing blood lipid. In the present invention, turmeric is used as the main raw material, supplemented by other raw materials, and the various raw materials act synergistically, which can reduce the contents of total cholesterol, total triglyceride, and low-density lipoprotein cholesterol in hyperlipidemic rats, increase the content of high-density lipoprotein cholesterol, and achieve the maintenance of a healthy blood lipid level.

[0035] 2. The lipid-lowering fermentation product provided by the present invention uses five raw materials, namely hawthorn, lotus leaf, tangerine peel, turmeric, and celery seeds. The raw materials are simple and all are raw materials that are both edible and medicinal, and can be applied to various fields such as food, health food, and medicine.

[0036] 3. Through the enzymolysis and fermentation processes, the present invention not only improves the utilization rate of raw materials, makes the active ingredients more easily absorbed by the human body, but also introduces probiotics and their metabolites, further enhancing the lipid-lowering effect of the composition and broadening the application prospects. Detailed implementation mode

[0037] The following non-limiting examples can enable those of ordinary skill in the art to more comprehensively understand the present invention, but do not limit the present invention in any way. The following content is only an exemplary illustration of the scope claimed by the present invention. Those skilled in the art can make various changes and modifications to the present invention based on the disclosed content, and it should also fall within the scope claimed by the present invention.

[0038] When the embodiments give numerical ranges, it should be understood that unless otherwise specified in the present invention, any value at both ends of each numerical range and any value between the two ends can be selected. Unless otherwise defined, all technical and scientific terms used in the present invention have the same meaning as commonly understood by those of ordinary skill in the art to which the present invention belongs.

[0039] The present invention will be further described by way of specific embodiments. All kinds of chemical reagents used in the embodiments of the present invention are obtained through conventional commercial channels unless otherwise specified. Products from different manufacturers have no significant impact on the effects.

[0040] Cellulase and acid protease were both purchased from Shandong Longkete Enzyme Preparation Co., Ltd.; papain was purchased from Guangxi Nanning Jevoli Biological Products Co., Ltd.; Lactobacillus rhamnosus and Lactobacillus casei were both purchased from Microkang Probiotics (Suzhou) Co., Ltd., Lactobacillus rhamnosus LRa66 and Lactobacillus casei LC89; Lactobacillus acidophilus was purchased from Shandong Zhongke Jiayi Bioengineering Co., Ltd., Lactobacillus acidophilus JYLA-126.

[0041] Example 1

[0042] This example provides a fermentation product with lipid-lowering efficacy, including 35 parts by weight of hawthorn, 30 parts by weight of lotus leaf, 20 parts by weight of tangerine peel, 60 parts by weight of turmeric, 15 parts by weight of celery seeds, 7 parts by weight of cellulase, 5 parts by weight of acid protease, 0.07 parts by weight of Lactobacillus rhamnosus, 0.04 parts by weight of Lactobacillus acidophilus, and 0.03 parts by weight of Lactobacillus casei. The enzyme activity of cellulase is 20,000 U / g, the enzyme activity of acid protease is 80,000 U / g, the viable count of Lactobacillus rhamnosus is 800 billion CFU / g, the viable count of Lactobacillus acidophilus is 500 billion CFU / g, and the viable count of Lactobacillus casei is 600 billion CFU / g.

[0043] Preparation method:

[0044] (1) 35 parts by weight of hawthorn, 30 parts by weight of lotus leaf, 20 parts by weight of tangerine peel, 60 parts by weight of turmeric, and 15 parts by weight of celery seeds are pulverized for 90 min at a temperature of 30°C, a pressure of 15 Mpa, and an air flow rate of 350 m / s to obtain micronized powder; (2) The micronized powder is mixed with 1840 parts by weight of water to obtain a traditional Chinese medicine mixture; (3) Sodium bicarbonate is added to the traditional Chinese medicine mixture to adjust the pH value to 5.6, and then 7 parts by weight of cellulase and 5 parts by weight of acid protease are added, and enzymolysis is carried out at a temperature of 55°C for 90 min to obtain an enzymolysis solution; (4) The enzymolysis solution is heated to 90°C and inactivated for 60 min to obtain an inactivated extraction solution; (5) After the inactivated extraction solution is cooled to 30°C, 0.07 parts by weight of Lactobacillus rhamnosus, 0.04 parts by weight of Lactobacillus acidophilus, and 0.03 parts by weight of Lactobacillus casei are added, and fermentation is carried out at 30°C for 24 h to obtain a fermentation broth; the fermentation broth is separated by solid-liquid separation to obtain the upper-layer fermentation supernatant, that is, the fermentation product.

[0045] Example 2

[0046] This embodiment provides a fermentation product with the efficacy of reducing blood lipid, including 20 parts by weight of hawthorn, 40 parts by weight of lotus leaf, 10 parts by weight of tangerine peel, 30 parts by weight of turmeric, 5 parts by weight of celery seeds, 6 parts by weight of cellulase, 4 parts by weight of acid protease, 0.02 parts by weight of Lactobacillus rhamnosus, 0.07 parts by weight of Lactobacillus acidophilus, and 0.01 parts by weight of Lactobacillus casei. Among them, the enzyme activity of cellulase is 20,000 U / g, the enzyme activity of acid protease is 80,000 U / g, the viable count of Lactobacillus rhamnosus is 800 billion CFU / g, the viable count of Lactobacillus acidophilus is 500 billion CFU / g, and the viable count of Lactobacillus casei is 600 billion CFU / g.

[0047] Preparation method:

[0048] (1) 20 parts by weight of hawthorn, 40 parts by weight of lotus leaf, 10 parts by weight of tangerine peel, 30 parts by weight of turmeric, and 5 parts by weight of celery seeds are pulverized for 60 min at a temperature of 20 °C, a pressure of 10 Mpa, and an air flow rate of 300 m / s to obtain micronized powder; (2) The micronized powder is mixed with 1895 parts by weight of water to obtain a traditional Chinese medicine mixture; (3) Sodium bicarbonate is added to the traditional Chinese medicine mixture to adjust the pH value to 5.6, and then 6 parts by weight of cellulase and 4 parts by weight of acid protease are added, and enzymolysis is carried out at a temperature of 40 °C for 100 min to obtain an enzymolysis solution; (4) The enzymolysis solution is heated to 80 °C and inactivated for 80 min to obtain an inactivated extraction solution; (5) After the inactivated extraction solution is cooled to 20 °C, 0.02 parts by weight of Lactobacillus rhamnosus, 0.07 parts by weight of Lactobacillus acidophilus, and 0.01 parts by weight of Lactobacillus casei are added, and fermentation is carried out at 20 °C for 32 h to obtain a fermentation broth; The fermentation broth is separated by solid-liquid separation to obtain the upper-layer fermentation supernatant, that is, the fermentation product.

[0049] Example 3

[0050] This embodiment provides a fermentation product with the efficacy of reducing blood lipid, including 30 parts by weight of hawthorn, 25 parts by weight of lotus leaf, 15 parts by weight of tangerine peel, 50 parts by weight of turmeric, 10 parts by weight of celery seeds, 5 parts by weight of cellulase, 8 parts by weight of acid protease, 0.04 parts by weight of Lactobacillus rhamnosus, 0.05 parts by weight of Lactobacillus acidophilus, and 0.04 parts by weight of Lactobacillus casei. Among them, the enzyme activity of cellulase is 20,000 U / g, the enzyme activity of acid protease is 80,000 U / g, the viable count of Lactobacillus rhamnosus is 800 billion CFU / g, the viable count of Lactobacillus acidophilus is 500 billion CFU / g, and the viable count of Lactobacillus casei is 600 billion CFU / g.

[0051] Preparation method:

[0052] (1) Take 30 parts by weight of hawthorn, 25 parts by weight of lotus leaf, 15 parts by weight of tangerine peel, 50 parts by weight of turmeric, and 10 parts by weight of celery seeds, and pulverize them for 60 min at a temperature of 25 °C, a pressure of 10 Mpa, and an air flow rate of 300 m / s to obtain micronized powder; (2) Mix the micronized powder with 1870 parts by weight of water to obtain a traditional Chinese medicine mixture; (3) Add sodium bicarbonate to the traditional Chinese medicine mixture to adjust the pH value to 5.6, then add 5 parts by weight of cellulase and 8 parts by weight of acid protease, and enzymolyze at a temperature of 60 °C for 70 min to obtain an enzymolyzed solution; (4) Heat the enzymolyzed solution to 85 °C and inactivate the enzyme for 70 min to obtain an enzyme-inactivated extract; (5) After the enzyme-inactivated extract is cooled to 35 °C, add 0.04 parts by weight of Lactobacillus rhamnosus, 0.05 parts by weight of Lactobacillus acidophilus, and 0.04 parts by weight of Lactobacillus casei, and ferment at 35 °C for 20 h to obtain a fermentation broth; Separate the solid and liquid of the fermentation broth to obtain the upper-layer fermentation supernatant, which is the fermentation product.

[0053] Example 4

[0054] This example provides a fermentation product with blood lipid-lowering efficacy, including 40 parts by weight of hawthorn, 35 parts by weight of lotus leaf, 25 parts by weight of tangerine peel, 70 parts by weight of turmeric, 20 parts by weight of celery seeds, 10 parts by weight of cellulase, 3 parts by weight of acid protease, 0.1 parts by weight of Lactobacillus rhamnosus, 0.03 parts by weight of Lactobacillus acidophilus, and 0.02 parts by weight of Lactobacillus casei. The enzyme activity of cellulase is 20,000 U / g, the enzyme activity of acid protease is 80,000 U / g, the viable count of Lactobacillus rhamnosus is 800 billion CFU / g, the viable count of Lactobacillus acidophilus is 500 billion CFU / g, and the viable count of Lactobacillus casei is 600 billion CFU / g.

[0055] Preparation method:

[0056] (1) Take 40 parts by weight of hawthorn, 35 parts by weight of lotus leaf, 25 parts by weight of tangerine peel, 70 parts by weight of turmeric, and 20 parts by weight of celery seeds, and pulverize them for 100 min at a temperature of 40 °C, a pressure of 15 Mpa, and an air flow rate of 400 m / s to obtain micronized powder; (2) Mix the micronized powder with 1810 parts by weight of water to obtain a traditional Chinese medicine mixture; (3) Add sodium bicarbonate to the traditional Chinese medicine mixture to adjust the pH value to 5.6, then add 10 parts by weight of cellulase and 3 parts by weight of acid protease, and enzymolyze at a temperature of 50 °C for 80 min to obtain an enzymolyzed solution; (4) Heat the enzymolyzed solution to 95 °C and inactivate the enzyme for 50 min to obtain an enzyme-inactivated extract; (5) After the enzyme-inactivated extract is cooled to 25 °C, add 0.1 parts by weight of Lactobacillus rhamnosus, 0.03 parts by weight of Lactobacillus acidophilus, and 0.02 parts by weight of Lactobacillus casei, and ferment at 25 °C for 30 h to obtain a fermentation broth; Separate the solid and liquid of the fermentation broth to obtain the upper-layer fermentation supernatant, which is the fermentation product.

[0057] Example 5

[0058] This embodiment provides a fermentation product with lipid-lowering efficacy, including 50 parts by weight of hawthorn, 20 parts by weight of lotus leaf, 30 parts by weight of tangerine peel, 90 parts by weight of turmeric, 25 parts by weight of celery seeds, 8 parts by weight of cellulase, 6 parts by weight of acid protease, 0.12 parts by weight of Lactobacillus rhamnosus, 0.01 parts by weight of Lactobacillus acidophilus, and 0.05 parts by weight of Lactobacillus casei. The enzyme activity of cellulase is 20,000 U / g, the enzyme activity of acid protease is 80,000 U / g, the viable count of Lactobacillus rhamnosus is 800 billion CFU / g, the viable count of Lactobacillus acidophilus is 500 billion CFU / g, and the viable count of Lactobacillus casei is 600 billion CFU / g.

[0059] Preparation method:

[0060] (1) 50 parts by weight of hawthorn, 20 parts by weight of lotus leaf, 30 parts by weight of tangerine peel, 90 parts by weight of turmeric, and 25 parts by weight of celery seeds are pulverized for 120 min at a temperature of 40 °C, a pressure of 15 Mpa, and an air flow rate of 400 m / s to obtain micronized powder; (2) the micronized powder is mixed with 1785 parts by weight of water to obtain a traditional Chinese medicine mixture; (3) sodium bicarbonate is added to the traditional Chinese medicine mixture to adjust the pH value to 5.6, and then 8 parts by weight of cellulase and 6 parts by weight of acid protease are added, and enzymolysis is carried out at a temperature of 65 °C for 50 min to obtain an enzymolysis solution; (4) the enzymolysis solution is heated to 100 °C and inactivated for 40 min to obtain an inactivated extraction solution; (5) after the inactivated extraction solution is cooled to 40 °C, 0.12 parts by weight of Lactobacillus rhamnosus, 0.01 parts by weight of Lactobacillus acidophilus, and 0.05 parts by weight of Lactobacillus casei are added, and fermentation is carried out at 40 °C for 16 h to obtain a fermentation broth; the fermentation broth is subjected to solid-liquid separation to obtain the upper-layer fermentation supernatant, which is the fermentation product.

[0061] Comparative Example 1

[0062] This comparative example provides a fermentation product with lipid-lowering efficacy, which is different from Example 1 in that it does not include tangerine peel, that is, it includes 35 parts by weight of hawthorn, 30 parts by weight of lotus leaf, 60 parts by weight of turmeric, and 15 parts by weight of celery seeds (the total weight of turmeric and celery seeds is the same as the total weight of turmeric, tangerine peel, and celery seeds in Example 1), 7 parts by weight of cellulase, 5 parts by weight of acid protease, 0.07 parts by weight of Lactobacillus rhamnosus, 0.04 parts by weight of Lactobacillus acidophilus, and 0.03 parts by weight of Lactobacillus casei. The enzyme activity of cellulase is 20,000 U / g, the enzyme activity of acid protease is 80,000 U / g, the viable count of Lactobacillus rhamnosus is 800 billion CFU / g, the viable count of Lactobacillus acidophilus is 500 billion CFU / g, and the viable count of Lactobacillus casei is 600 billion CFU / g.

[0063] Its preparation method is the same as that of Example 1.

[0064] Comparative Example 2

[0065] This comparative example provides a fermentation product with lipid-lowering efficacy. The difference from Example 1 is that it does not include turmeric, that is, it includes 35 parts by weight of hawthorn, 30 parts by weight of lotus leaf, 20 parts by weight of tangerine peel, 15 parts by weight of celery seeds (the total weight of tangerine peel and celery seeds is the same as the total weight of turmeric, tangerine peel and celery seeds in Example 1), 7 parts by weight of cellulase, 5 parts by weight of acid protease, 0.07 parts by weight of Lactobacillus rhamnosus, 0.04 parts by weight of Lactobacillus acidophilus, and 0.03 parts by weight of Lactobacillus casei. Among them, the enzyme activity of cellulase is 20,000 U / g, the enzyme activity of acid protease is 80,000 U / g, the viable count of Lactobacillus rhamnosus is 800 billion CFU / g, the viable count of Lactobacillus acidophilus is 500 billion CFU / g, and the viable count of Lactobacillus casei is 600 billion CFU / g.

[0066] Its preparation method is the same as that of Example 1.

[0067] Comparative Example 3

[0068] This comparative example provides a fermentation product with lipid-lowering efficacy. The difference from Example 1 is that it does not include celery seeds, that is, it includes 35 parts by weight of hawthorn, 30 parts by weight of lotus leaf, 20 parts by weight of tangerine peel, 60 parts by weight of turmeric (the total weight of tangerine peel and turmeric is the same as the total weight of tangerine peel, turmeric and celery seeds in Example 1), 7 parts by weight of cellulase, 5 parts by weight of acid protease, 0.07 parts by weight of Lactobacillus rhamnosus, 0.04 parts by weight of Lactobacillus acidophilus, and 0.03 parts by weight of Lactobacillus casei. Among them, the enzyme activity of cellulase is 20,000 U / g, the enzyme activity of acid protease is 80,000 U / g, the viable count of Lactobacillus rhamnosus is 800 billion CFU / g, the viable count of Lactobacillus acidophilus is 500 billion CFU / g, and the viable count of Lactobacillus casei is 600 billion CFU / g.

[0069] Its preparation method is the same as that of Example 1.

[0070] Comparative Example 4

[0071] This comparative example provides a fermentation product with lipid-lowering efficacy. The difference from Example 1 is that tangerine peel is replaced with dried tangerine peel, that is, it includes 35 parts by weight of hawthorn, 30 parts by weight of lotus leaf, 20 parts by weight of dried tangerine peel, 60 parts by weight of turmeric, 15 parts by weight of celery seeds, and the remaining components and preparation method are the same as those in Example 1.

[0072] Comparative Example 5

[0073] This comparative example provides a fermentation product with lipid-lowering efficacy. The difference from Example 1 is that the acid protease is replaced with papain, and the remaining components and preparation method are the same as those in Example 1. The enzyme activity of the papain is 80,000 U / g.

[0074] Comparative Example 6

[0075] This comparative example provides a fermentation product with lipid-lowering efficacy. The difference from Example 1 is that the starter culture only includes 0.14 parts by weight of Lactobacillus casei rhamnosus, and the remaining components and preparation method are the same as those in Example 1. The viable count of Lactobacillus casei rhamnosus is 800 billion CFU / g.

[0076] Comparative Example 7

[0077] This comparative example provides a fermentation product with lipid-lowering efficacy. The difference from Example 1 is that the starter culture does not include Lactobacillus casei rhamnosus, but includes 0.08 parts by weight of Lactobacillus acidophilus and 0.06 parts by weight of Lactobacillus casei. The remaining components and preparation method are the same as those in Example 1. The viable count of Lactobacillus acidophilus is 500 billion CFU / g, and the viable count of Lactobacillus casei is 600 billion CFU / g.

[0078] Comparative Example 8

[0079] This comparative example provides a product with lipid-lowering efficacy. The difference from Example 1 is that it is not subjected to fermentation treatment.

[0080] Preparation method:

[0081] (1) Pulverize 35 parts by weight of hawthorn, 30 parts by weight of lotus leaf, 20 parts by weight of tangerine peel, 60 parts by weight of turmeric, and 15 parts by weight of celery seeds for 90 min at a temperature of 30°C, a pressure of 15 Mpa, and an air flow rate of 350 m / s to obtain micronized powder; (2) Mix the micronized powder with 1840 parts by weight of water to obtain a traditional Chinese medicine mixture; (3) Adjust the pH value of the traditional Chinese medicine mixture to 5.6 by adding sodium bicarbonate, then add 7 parts by weight of cellulase and 5 parts by weight of acid protease, and enzymatically hydrolyze for 90 min at a temperature of 55°C to obtain an enzymatic hydrolysate; (4) Heat the enzymatic hydrolysate to 90°C and inactivate the enzyme for 60 min to obtain an enzyme-inactivated extract; (5) Separate the solid and liquid of the enzyme-inactivated extract to obtain the supernatant, which is the product.

[0082] Comparative Example 9

[0083] This comparative example provides a product with lipid-lowering efficacy. The difference from Example 1 is that it is not subjected to enzymatic hydrolysis and fermentation treatment.

[0084] Preparation method:

[0085] (1) Crush 35 parts by weight of hawthorn, 30 parts by weight of lotus leaf, 20 parts by weight of tangerine peel, 60 parts by weight of turmeric, and 15 parts by weight of celery seeds for 90 min at a temperature of 30 °C, a pressure of 15 Mpa, and an air flow rate of 350 m / s to obtain a micronized powder; (2) Mix the micronized powder with 1840 parts by weight of water to obtain a traditional Chinese medicine mixture; (3) Separate the solid and liquid of the traditional Chinese medicine mixture to obtain the supernatant, which is the traditional Chinese medicine product.

[0086] Comparative Example 10

[0087] This comparative example provides a fermentation product with lipid-lowering efficacy. The difference from Example 1 is the different proportions of tangerine peel, turmeric, and celery seeds. Specifically, it includes 35 parts by weight of hawthorn, 30 parts by weight of lotus leaf, 80 parts by weight of tangerine peel, 12 parts by weight of turmeric, 3 parts by weight of celery seeds, 7 parts by weight of cellulase, 5 parts by weight of acid protease, 0.07 parts by weight of Lactobacillus rhamnosus, 0.04 parts by weight of Lactobacillus acidophilus, and 0.03 parts by weight of Lactobacillus casei. The enzyme activity of cellulase is 20,000 U / g, the enzyme activity of acid protease is 80,000 U / g, the viable count of Lactobacillus rhamnosus is 800 billion CFU / g, the viable count of Lactobacillus acidophilus is 500 billion CFU / g, and the viable count of Lactobacillus casei is 600 billion CFU / g.

[0088] Its preparation method is the same as that of Example 1.

[0089] Experimental Example 1 Animal Experiment

[0090] The ELISA kits for detecting total cholesterol (TC) in rats (product number: CB11076-Ra), triglyceride (TG) in rats (product number: CB10370-Ra), low-density lipoprotein cholesterol (LDL-C) in rats (product number: CB10309-Ra), and high-density lipoprotein cholesterol (HDL-C) in rats (product number: CB10384-Ra) were all purchased from Shanghai Kaitai Biotechnology Co., Ltd.; cholesterol (99%): food grade, purchased from Anhui Yuanzheng Bioengineering Co., Ltd.; sodium deoxycholate (99%): food grade, purchased from Anhui Yuanzheng Bioengineering Co., Ltd.

[0091] To verify the lipid-lowering effect of the fermentation product of the present invention, the following is an explanation of the animal experiment:

[0092] Animals: Healthy male Wistar rats, weighing 180 - 200 g, 8 - 10 weeks old.

[0093] The ratio of the high-fat diet is lard: cholesterol: bile salt: basal diet = 10: 1.5: 0.5: 88.

[0094] Rats were kept in an animal laboratory with a breeding temperature of 24±2°C, a relative humidity of 40%, and a 12-hour light-dark cycle, and were allowed free access to food and water. After 1 week of adaptation, the rats were divided into two groups. The first group consisted of 10 rats as the normal group, which were fed a basal diet at 100 g / kg / d. The remaining rats (160 rats) were used to establish a high-fat model and were fed a high-fat diet at 100 g / kg / d. After continuous feeding for 4 weeks, the rats in each group were fasted for 12 hours, and blood was collected from the tail vein. Serum was rapidly separated, and blood lipid indices such as TG and TC were measured. The levels of TC, TG, and LDL-C were all significantly increased, while the level of HDL-C was significantly decreased, indicating that the model was successfully established.

[0095] The rats with successful model establishment were randomly divided into 16 groups, namely the model group, Examples 1-5, and Comparative Examples 1-10, with 10 rats in each group. The normal group was fed a basal diet at 100 g / kg / d, and the rats in the model group were fed a high-fat diet at 100 g / kg / d at the same time every day. Meanwhile, Examples 1-5 and Comparative Examples 1-10 were intragastrically administered the corresponding fermentation products, while the model group and the normal group were intragastrically administered an equal amount of normal saline. Intragastric administration was carried out continuously for 4 weeks. After 4 weeks of feeding and intragastric administration, the rats were fasted for 12 hours without water restriction, and blood was collected from the abdominal aorta. Serum was separated, and a kit was used to measure the levels of TC, TG, HDL-C, and LDL-C in the serum.

[0096] The administration protocol was to perform intragastric administration once at a fixed time every day. According to the equivalent dose method calculated by the body surface area ratio, the intragastric administration dose for rats was 18 mL / kg based on a human daily dose of 200 mL, and intragastric administration was carried out according to this dose.

[0097] Table 1 Effects of Fermentation Products of Each Group on Blood Lipids in Hyperlipidemic Rats

[0098]

[0099] Note: Compared with the normal group, in the model group: # indicates P<0.05, ## represents P<0.01; compared with the model group, in Examples 1-5 and Comparative Examples 1-10: + represents P<0.05, ++ represents P<0.01; compared with Example 1, in Comparative Examples 1-10, △ represents P<0.05, △△ represents P<0.01.

[0100] According to the results in Table 1, compared with the normal group, after the rats in the model group were fed a high-fat diet for 4 weeks, the serum TC, TG, and LDL-C were all significantly increased, while the HDL-C was significantly decreased, indicating that the model was successfully established.

[0101] Examples 1-5 used the fermentation products of hawthorn, lotus leaf, tangerine peel, turmeric, and celery seed. Compared with the model group, the TC, TG, and LDL-C of the rats decreased significantly, and the HDL-C increased significantly, indicating that the fermentation products provided in Examples 1-5 of the present invention all have the effect of reducing blood lipids.

[0102] The differences between Comparative Examples 1-3 and Example 1 are that one of the raw materials, namely, tangerine peel, turmeric or celery seeds, is missing. The difference between Comparative Example 4 and Example 1 is that tangerine peel is replaced with dried tangerine peel. From the experimental results, it can be seen that the TC, TG, and LDL-C of the rats in Comparative Examples 1-4 increased significantly, while the HDL-C decreased significantly. This shows that the combined action of hawthorn, lotus leaf, tangerine peel, turmeric, and celery seeds can achieve a better effect of reducing blood lipids.

[0103] Compared with Example 1, in Comparative Examples 5-7, the TC, TG, and LDL-C of the rats increased to varying degrees, and the HDL-C decreased to varying degrees, indicating that the selection of enzyme preparations and fermenting agents also has a certain impact on reducing blood lipids. The combination of fermenting agent and enzyme preparation selected in the present invention can well exert the efficacy of reducing blood lipids.

[0104] Compared with Example 1, in Comparative Examples 8-9, the TC, TG, and LDL-C of the rats increased significantly, while the HDL-C decreased significantly, indicating that the enzymolysis fermentation technology adopted in the present invention can further improve the efficacy of the fermentation product in reducing blood lipids.

[0105] The difference between Comparative Example 10 and Example 1 is that the proportion of tangerine peel is increased and the ratio of tangerine peel, turmeric, and celery seeds is changed. From the experimental results, it can be seen that the TC, TG, and LDL-C of the rats increased significantly, while the HDL-C decreased, indicating that changing the ratio of raw materials will also affect the blood lipid reduction effect, and the fermentation product provided by the present invention can better exert its efficacy of reducing blood lipids.

[0106] Finally, it should be noted that the above content is only used to illustrate the technical solution of the present invention, rather than to limit the protection scope of the present invention. Any simple modification or equivalent replacement of the technical solution of the present invention by those of ordinary skill in the art shall not depart from the essence and scope of the technical solution of the present invention.

Claims

1. A fermentation product with the efficacy of reducing blood lipid, characterized in that, By weight parts, it includes 20 - 50 parts of hawthorn, 20 - 40 parts of lotus leaf, 10 - 30 parts of tangerine peel, 30 - 90 parts of turmeric, 5 - 25 parts of celery seeds, 8 - 18 parts of enzyme preparation and 0.04 - 0.24 parts of fermenting agent.

2. The lipid-lowering fermentation product according to claim 1, wherein The fermenting agent, by weight parts, includes 0.02 - 0.12 parts of Lactobacillus rhamnosus, 0.01 - 0.07 parts of Lactobacillus acidophilus, and 0.01 - 0.05 parts of Lactobacillus casei.

3. The lipid-lowering fermentation product according to claim 1, wherein The enzyme preparation, by weight parts, includes 5 - 10 parts of cellulase and 3 - 8 parts of acid protease.

4. The lipid-lowering fermentation product according to claim 1, wherein, The weight ratio of the hawthorn, lotus leaf, tangerine peel, turmeric and celery seeds is (30 - 40):(25 - 35):(15 - 25):(50 - 70):(10 - 20).

5. The lipid-lowering fermentation product according to claim 1, wherein, The weight ratio of the turmeric and tangerine peel is (2 - 3.5):1; The weight ratio of the turmeric and lotus leaf is (0.75 - 4.5):1; The weight ratio of the turmeric and celery seeds is (3.5 - 6):

1.

6. The lipid-lowering fermentation product according to claim 1, wherein The weight ratio of the hawthorn, lotus leaf, tangerine peel, turmeric and celery seeds is 35:30:20:60:

15.

7. The preparation method of the fermentation product with lipid-lowering effect according to any one of claims 1-6, characterized in that, It includes the following steps: (1) Crush the traditional Chinese medicine composition to obtain micronized powder; mix the micronized powder with water to form a traditional Chinese medicine mixed solution; (2) Adjust the pH of the traditional Chinese medicine mixed solution, and then add the enzyme preparation for enzymatic hydrolysis treatment to obtain an enzymatic hydrolysate; (3) Heat up the enzymatic hydrolysate for enzyme inactivation extraction to obtain an enzyme-inactivated extraction solution; (4) Add the fermenting agent to the enzyme-inactivated extraction solution for fermentation to obtain a fermentation broth; perform solid-liquid separation on the fermentation broth to obtain a fermentation clear liquid, which is the fermentation product.

8. The preparation method of the fermentation product according to claim 7, characterized in that, In the step (2), the temperature of enzymatic hydrolysis is 40 - 65°C, and the enzymatic hydrolysis time is 50 - 100 min; in the step (3), the enzyme inactivation extraction temperature is 80 - 100°C, and the enzyme inactivation extraction time is 40 - 80 min; in the step (4), the fermentation temperature is 20 - 40°C, and the fermentation time is 16 - 32 h.

9. The preparation method of the fermentation product according to claim 7, characterized in that, In the step (1), the conditions for crushing are: crushing temperature 20 - 40°C, crushing pressure 10 - 15 Mpa, air flow rate 300 - 400 m / s, and crushing time 1 - 2 h.

10. Use of the fermentation product according to any one of claims 1 - 6 or the fermentation product obtained by the preparation method according to claims 7 - 9 in the preparation of a hypolipidemic product.

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