Anaerobic biological growth promoter and preparation method thereof

Abstract

The present application relates to sewage treatment field, disclose a kind of anaerobic biological growth promoter and its preparation method.An aerobic biological growth promoter includes the following weight parts components: enzyme: 4~6 parts;Exogenous regulating hormone: 0.5~1 parts;Organic salt: 1~2 parts;Inorganic salt: 0.25~0.75 parts;Slow-release carrier: 2~3 parts.This anaerobic biological growth promoter has environmental stability, can keep performance stable within certain pH and temperature range, also can be used as anaerobic microbial carrier, significantly improve the tolerance of anaerobic microorganism to environmental conditions, and continuously supply nutrient through slow-release effect, improve the metabolic activity of anaerobic microorganism, make its sewage treatment efficiency significantly improve, effectively solve the problem of traditional anaerobic biological treatment system slow start, easy to lose stability.

Description

TECHNICAL FIELD

[0001] The present application relates to the field of sewage treatment, in particular to an anaerobic biological growth promoter and a preparation method thereof. BACKGROUND

[0002] In modern sewage treatment processes, biochemical treatment plays a crucial role as a core link. This technology mainly utilizes the metabolic action of specific microbial populations to convert large molecular organic matter and toxic and harmful substances in sewage that are difficult to degrade by physical and chemical methods into small molecular substances or harmless components, thereby significantly reducing their concentration and making the effluent water quality meet the discharge requirements. Biochemical treatment processes are mainly divided into anaerobic biological treatment and aerobic biological treatment, among which anaerobic biological treatment is a process in which complex organic matter is gradually decomposed into simple substances such as methane and carbon dioxide through the synergistic action of hydrolytic bacteria, acidifying bacteria and methanogenic bacteria under anaerobic conditions.

[0003] However, anaerobic biological treatment technology still faces many challenges in practical application: first, the generation period of anaerobic microorganisms is long, resulting in slow system startup, which usually takes 2-3 months to reach a stable running state; second, the anaerobic biological treatment system has poor impact resistance, anaerobic microorganisms are extremely sensitive to environmental conditions, with a narrow optimal temperature range, a small pH tolerance range, and are easily inhibited by toxic substances such as heavy metals and sulfides. Therefore, how to improve the metabolic activity and environmental adaptability of anaerobic microorganisms has become a problem to be solved in current anaerobic biological treatment.

[0004] Biological growth promoters enhance microbial activity and improve system stability and impact resistance by supplying water treatment microorganisms with substances required for life activities. CN102515364B discloses a broad-spectrum microbial promoter for sewage treatment, which can achieve a COD removal rate of 85.52% after adding the promoter; CN110040846B discloses a promoter for enhancing the denitrification reaction of sewage treatment, which contains a high proportion of polyols, although it can improve the denitrification reaction, but it will cause osmotic stress to anaerobic microorganisms in sewage treatment, affecting the metabolic activity of anaerobic microorganisms. In summary, the existing biological growth promoter technology lacks targeted development for the physiological characteristics of anaerobic microorganisms, and is difficult to meet the special needs of anaerobic microorganisms. SUMMARY

[0005] In order to solve the problems of slow start and poor impact capacity of the anaerobic biological wastewater treatment system, the present application provides an anaerobic biological growth promoter and a preparation method thereof, the anaerobic biological growth promoter with a slow-release carrier has high efficiency, stability and specificity, can provide a suitable microenvironment and required nutrients for anaerobic microorganisms to improve the metabolic activity of the microorganisms, and can build a buffer system to improve the adaptability of the anaerobic microorganisms to different wastewater environments, and can prolong the effect time of the growth promoter by building a microcapsule structure, and comprehensively improve the anaerobic biological wastewater treatment efficiency.

[0006] The technical scheme adopted by the present application to achieve the above-mentioned purpose is:

[0007] The present application provides an anaerobic biological growth promoter, which comprises the following components by weight: enzymes: 4-6 parts; exogenous regulatory hormones: 0.5-1 part; organic salts: 1-2 parts; inorganic salts: 0.25-0.75 parts; slow-release carriers: 2-3 parts.

[0008] Preferably, the enzymes are cellulase, hydrogenase and protease, and the weight ratio of cellulase, hydrogenase and protease is 3:1:1. The enzymes in this ratio can provide substrates for anaerobic bacteria, enhance the activity of anaerobic bacteria and improve the stability of the system.

[0009] Preferably, the exogenous regulatory hormones are one or more of cobalamin, thiamine or hematin, and the organic salts are one or more of ammonium formate, ammonium acetate, calcium acetate or calcium gluconate. The hormones can enhance the organic matter degradation efficiency of anaerobic bacteria, and the organic salts provide nitrogen / carbon sources to improve the activity of anaerobic bacteria.

[0010] Preferably, the inorganic salts comprise sulfate and phosphate, and the weight ratio of sulfate and phosphate is 2-4:1. The organic salts and inorganic salts form a buffer system by matching, thereby reducing the influence of environmental pH on anaerobic bacteria.

[0011] More preferably, the sulfate is one or more of ferrous sulfate, magnesium sulfate or sodium sulfate, and the phosphate is one or more of potassium dihydrogen phosphate or sodium dihydrogen phosphate. The inorganic salts provide metal elements for anaerobic bacteria to meet the nutritional requirements of the anaerobic bacteria.

[0012] Preferably, the slow-release carrier is a complex system formed by rhamnolipid and chitosan, and the weight ratio of rhamnolipid to chitosan is 1:1-2.

[0013] More preferably, the slow-release carrier forms a microcapsule through the three-dimensional network structure of chitosan molecules, and a dense molecular layer of rhamnolipid is formed on the surface of the microcapsule to construct a slow-release microcapsule structure. The slow-release microcapsule structure can embed other components of the growth promoter and protect anaerobic microorganisms as a carrier.

[0014] The present application also provides a preparation method of the anaerobic microbial growth promoter, which comprises the following steps:

[0015] The preparation method comprises the following steps: adding the slow-release carrier into water and mixing, adding inorganic salt, exogenous regulating hormone, organic salt and enzyme into the solution, and mixing to obtain the biological growth promoter.

[0016] More preferably, after the slow-release carrier is added into water, ultrasonic treatment is used for mixing; the ultrasonic treatment power is 200-400 W, the temperature is 20-30 ℃, and the time is 30-60 min. The ultrasonic treatment modifies the slow-release carrier, and the slow-release carrier has stronger embedding function and slow-release effect.

[0017] More preferably, the adding step is performed in the order of inorganic salt, exogenous regulating hormone, organic salt and enzyme, and the mixing and stirring time is 20-30 min.

[0018] Compared with the prior art, the present application has the following advantages: (1) the present application selects the components of the growth promoter, improves the metabolic activity of anaerobic microorganisms, and uses ultrasonic treatment to optimize the microcapsule structure of the chitosan-rhamnolipid composite system, which significantly improves the embedding efficiency of the composition on other components of the growth promoter, further enhances the metabolic activity of anaerobic microorganisms in the wastewater treatment process, and significantly improves the wastewater treatment efficiency of the anaerobic microorganisms. (2) The composite prepared by the present application has excellent environmental stability and can maintain stable performance in a wider pH range and temperature range. As a biological carrier of anaerobic microorganisms, the composite significantly improves the tolerance of the microorganisms to environmental conditions, so that the microorganisms can maintain high activity under conditions of temperature fluctuation and pH fluctuation. (3) The rhamnolipid and chitosan in the composite form a stable slow-release microcapsule structure through multiple intermolecular interactions, which not only provides a suitable growth microenvironment for anaerobic microorganisms, but also continuously supplies nutrients through slow release, effectively solving the problems of slow start and instability of traditional anaerobic biological treatment systems. DETAILED DESCRIPTION

[0019] The present application will be further described below in combination with examples:

[0020] Overall examples

[0021] The present application provides an anaerobic biological growth promoter, which comprises the following components by weight: enzyme: 4-6 parts; exogenous regulating hormone: 0.5-1 part; organic salt: 1-2 parts; inorganic salt: 0.25-0.75 parts; slow-release carrier: 2-3 parts.

[0022] In some preferred embodiments, the enzymes are cellulase, hydrogenase and protease; the weight ratio of cellulase, hydrogenase and protease is 3:1:1. The technical effect lies in that cellulase provides easily degradable substrates for anaerobic bacteria by hydrolyzing polysaccharides, shortens the start-up period of anaerobic digestion and improves substrate utilization rate; protease hydrolyzes macromolecular proteins to avoid inhibition of long-chain proteins and maintain system stability; hydrogenase can strengthen methanogens and improve the degradation efficiency of methanogens.

[0023] In some preferred embodiments, the exogenous regulatory hormones are one or more of cobalamin, thiamine or heme; and the organic salts are one or more of ammonium formate, ammonium acetate, calcium acetate or calcium gluconate. The technical effect lies in that the exogenous regulatory hormones and the organic salts optimize the activity of wastewater treatment microorganisms through the same mechanism, the hormones activate the function of key coenzymes, the organic salts provide nitrogen source / carbon source and stabilize cell structure, and the two synergistically enhance the degradation efficiency of pollutants and maintain the stability of anaerobic environment.

[0024] In some preferred embodiments, the inorganic salts comprise sulfate and phosphate; and the weight ratio of sulfate and phosphate is 2-4:1. The technical effect lies in that in an anaerobic environment, sulfate and phosphate form a dynamic buffer system, effectively maintaining the stability of the environment pH and ensuring the metabolic activity of anaerobic bacteria. Sulfate consumes protons and generates alkaline substances through biological reduction process, alleviating acidification caused by organic acid accumulation; phosphate directly neutralizes excess hydrogen ions through dissociation equilibrium, playing a core buffering role in the neutral to weak alkaline range. The two complement each other to cover a wider pH range, while avoiding the risk of sulfide toxicity.

[0025] In some more preferred embodiments, the sulfate is one or more of ferrous sulfate, magnesium sulfate or sodium sulfate; and the phosphate is one or more of potassium dihydrogen phosphate or sodium dihydrogen phosphate. The technical effect lies in that the sulfate provides sulfur elements and metal ions, activates dehydrogenase and coenzyme activity; the phosphate supplements phosphorus nutrition and regulates the nitrogen-phosphorus ratio, supporting nucleic acid synthesis and energy metabolism. The synergistic action of the two can meet the nutritional needs of anaerobic microorganisms and improve the degradation efficiency of organic matter.

[0026] In some preferred embodiments, the slow-release carrier is a composite system formed by rhamnolipid and chitosan, and the weight ratio of rhamnolipid and chitosan is 1:1-2.

[0027] In some more preferred embodiments, the slow-release carrier forms microcapsules through the three-dimensional network structure of chitosan molecules, and a dense molecular layer of rhamnolipid is formed on the surface of the microcapsules, forming a slow-release microcapsule structure. The technical effect is that the constructed slow-release carrier constructs a double protection system through the molecular synergy of chitosan and rhamnolipid; the chitosan molecules are cross-linked through electrostatic interaction and hydrogen bond to form a three-dimensional network microcapsule, realizing efficient embedding of other components of the biological growth promoter; the self-assembled rhamnolipid molecular layer on the surface of the microcapsule effectively blocks the damage of external dissolved oxygen, free radicals and extreme pH / temperature to the active ingredients through hydrophobic barrier and charge shielding effect; the slow-release carrier has physical isolation and chemical stability functions, and its porous structure provides an ideal attachment interface for anaerobic microorganisms; under environmental stress, the microcapsule interior maintains local microenvironment homeostasis through ion exchange with the embedded chemical buffer system, and releases active substances on demand through the pore diffusion mechanism, so that the anaerobic microorganisms can still maintain metabolic activity when responding to acid-base impact and temperature fluctuations.

[0028] The application further provides a preparation method of the anaerobic biological growth promoter, specifically comprising the following steps: adding the slow-release carrier into water and mixing, and then adding inorganic salt, exogenous regulating hormone, organic salt and enzyme into the solution to obtain the biological growth promoter.

[0029] In some preferred embodiments, after the slow-release carrier is added into water, ultrasonic treatment is used for uniform mixing; the ultrasonic treatment power is 200-400 W, the temperature is 20-30 DEG C, and the time is 30-60 min. The technical effect is that the performance of the chitosan and rhamnolipid microcapsule system is significantly improved through the unique cavitation effect, mechanical shearing and microjet action of ultrasonic treatment; under the action of ultrasonic treatment, the local high-temperature and high-pressure environment generated by the rupture of cavitation bubbles makes the chitosan molecular chain restructure, so that the molecular weight is reduced and the degree of deacetylation is improved, thereby significantly improving the solubility and reactivity; under the strong shearing action of ultrasonic treatment, the size of the rhamnolipid micelles is reduced, and the dispersibility is obviously improved; the active radicals generated during the ultrasonic treatment process initiate the oxidative degradation of chitosan, promote the oxidative modification of rhamnolipid, and activate the functional groups of chitosan and rhamnolipid, so that more hydrogen bonds and electrostatic interactions are formed between the amino and hydroxyl groups of chitosan and the carboxyl groups of rhamnolipid, and the complexing efficiency is improved; the physical-chemical synergistic effect makes the structure of the microcapsule more stable, the particle size distribution more uniform, the embedding efficiency of anaerobic microorganisms higher, and the slow-release performance significantly enhanced.

[0030] In some preferred embodiments, the adding step adds in order of inorganic salt, exogenous regulating hormone, organic salt and enzyme, and the mixing stirring time is 20-30 min. The technical effect is that in the preparation process, the substances are added in order of inorganic salt, exogenous regulating hormone, organic salt and enzyme, which can maximize the synergistic effect of each component, and ensure the stability and high efficiency of the preparation performance; first adding inorganic salt can establish a stable ionic environment, adjust the osmotic pressure and pH value of the preparation, and provide suitable physical and chemical conditions for the addition of subsequent components; secondly adding exogenous regulating hormone can accurately regulate the physiological state of the active ingredients in the preparation; then adding organic salt can serve as a buffer to maintain the stability of the preparation, and provide necessary carbon source and energy material for anaerobic bacteria, and enhance its metabolic activity; finally adding enzyme preparation can optimize the catalytic performance of the preparation, and improve the degradation efficiency of the bacteria on the target substrate. DETAILED DESCRIPTION

[0032] Preparation of the composition

[0033] Example 1

[0034] Step 1: 20 g of rhamnolipid and 30 g of chitosan were added to deionized water. An ultrasonic processor was used, with a power of 300 W, a temperature of 25°C, and a processing time of 40 minutes.

[0035] Step 2: 7.5 g of ferrous sulfate and 2.5 g of potassium dihydrogen phosphate were added to the above solution. A magnetic stirrer was used to stir at room temperature for 5 minutes to ensure that the inorganic salt was completely dissolved.

[0036] Step 3: 15 g of thiamine was added and stirred for 5 minutes to ensure uniform dispersion of the exogenous regulating hormone.

[0037] Step 4: 30 g of ammonium acetate was added and stirred for 10 minutes to ensure that the organic salt was completely dissolved.

[0038] Step 5: Finally, 60 g of cellulase, 20 g of hydrogenase and 20 g of protease were added. Stirring for 15 minutes ensured that the enzymes were uniformly dispersed in the solution.

[0039] Comparative Example 1

[0040] The growth promoter was prepared using the formula and preparation method of CN102515364B.

[0041] Comparative Example 2

[0042] The difference from Example 1 is that rhamnolipid and chitosan are not added in the preparation process of the growth promoter, and the solution is not subjected to ultrasonic treatment.

[0043] Example 2

[0044] The difference from Example 1 is that no ultrasonic treatment is performed after adding rhamnolipid and chitosan in Step 1.

[0045] Example 3

[0046] The difference from Example 1 is that 20 g of rhamnolipid and 20 g of chitosan are added in Step 1.

[0047] Example 4

[0048] The difference from Example 1 is that 20 g of rhamnolipid and 10 g of chitosan are added in Step 1.

[0049] Example 5

[0050] The difference from Example 1 is that the ultrasonic treatment machine parameters are set as follows in Step 1: power 200 W, temperature 20℃, and treatment time 40 min.

[0051] Example 6

[0052] The difference from Example 1 is that the ultrasonic treatment machine parameters are set as follows in Step 1: power 300 W, temperature 25℃, and treatment time 50 min.

[0053] Example 7

[0054] The difference from Example 1 is that the ultrasonic treatment machine parameters are set as follows in Step 1: power 400 W, temperature 30℃, and treatment time 50 min.

[0055] Example 8

[0056] The difference from Example 1 is that 5 g of ferrous sulfate and 2.5 g of potassium dihydrogen phosphate are added in Step 2.

[0057] Example 9

[0058] The difference from Example 1 is that 10 g of ferrous sulfate and 2.5 g of potassium dihydrogen phosphate are added in Step 2.

[0059] Example 10

[0060] The difference from Example 1 is that the hormone added in Step 3 is cobalamin.

[0061] Example 11

[0062] The difference from Example 1 is that the hormone added in Step 3 is hematin.

[0063] Example 12

[0064] The difference from Example 1 is that the organic acid added in Step 4 is calcium acetate.

[0065] Example 13

[0066] The difference from Example 1 is that the organic acid added in step 4 is ammonium formate.

[0067] Example 14

[0068] The difference from Example 1 is that the organic acid added in step 4 is calcium gluconate.

[0069] Example 15

[0070] The difference from Example 1 is that the mass of cellulase added in step 5 is 40 g.

[0071] Example 16

[0072] The difference from Example 1 is that the mass of cellulase added in step 5 is 80 g.

[0073] Table 1 Formulation summary

[0074]

[0075] The number in the table represents that the substance is not added or the operation is not performed.

[0076] Examples 1-7 and Comparative Example 2 are the screening of rhamnolipid and chitosan ratio and ultrasonic treatment parameters.

[0077] Examples 1, Examples 8-16 are the screening of active substance formulations of growth promoters.

[0078] Performance test

[0079] (1) Collect the mixed sewage sample after the coarse grid catch basin, add it to a 10L small anaerobic reactor, and strictly control the reaction conditions according to the actual operation parameters of the anaerobic tank of the sewage plant: the dissolved oxygen content is maintained at ≤0.1 mg / L by nitrogen blowing, the pH value is stabilized at 7-7.5 by an automatic control system, the temperature is controlled at 35.0±0.5℃ by a constant temperature water bath jacket, and the hydraulic retention time is fixed at 3 hours; all reactors are synchronously dosed with equal quality of anaerobic bacteria, and then equal quality of pretreated growth promoters (Examples 1-16 and Comparative Examples 1-2) or equal volume of deionized water (control group) are injected, the whole process is mechanically stirred at 150 rpm to ensure uniform mixing, and the constant temperature operation is carried out; samples are collected before and after treatment, and the solid suspended substance (SS, GB / T 1901-1989 gravimetric method) and chemical oxygen demand (COD, HJ828-2017 dichromate method) are determined according to the national standard method, and the degradation rate is calculated according to the formula:

[0080] Suspended solids degradation rate (%) = (1-initial SS / final SS after treatment) x 100;

[0081] Organic matter degradation rate (%) = (1-initial COD / final COD after treatment) x 100; the results are shown in Table 2.

[0082] Table 2

[0083]

[0084] As can be seen from Table 2, the anaerobic biological growth promoter provided by the embodiments of the present application is used for sewage treatment, and under the condition of fixed hydraulic retention time, the degradation efficiency of suspended solids and organic matter is significantly improved compared with the comparative examples and the control group.

[0085] Comparative Example 1-2 does not contain a slow-release carrier, and its degradation rate is higher than that of the control group, but its degradation efficiency is significantly lower than that of the embodiments; after introducing the slow-release carrier, the SS / organic matter degradation rate of Example 2 is improved compared with Comparative Example 1-2; and the SS / organic matter degradation rate of Example 3 is significantly improved after ultrasonic treatment of the slow-release carrier, indicating that ultrasonic induction of chitosan molecular chain reconstruction and combination with rhamnolipid to construct a microcapsule structure enhances the embedding efficiency of other components of the growth promoter and improves the slow-release effect of the active components; this synergistic effect is further verified in Examples 3-7.

[0086] Examples 1, 8-16 prove that the preferred formula of the present application can significantly improve the SS / organic matter degradation rate compared with the control group, and the most optimal formula is 3 parts of cellulase, 1 part of hydrogenase, 1 part of protease as enzymes, 0.75 parts of thiamine as a hormone, 1.5 parts of ammonium acetate as an organic salt, 0.375 parts of ferrous sulfate and 0.125 parts of potassium dihydrogen phosphate as inorganic salts, 1 part of rhamnolipid and 1.5 parts of chitosan as slow-release carriers, and the prepared growth promoter has the best effect. The selected enzyme ratio improves the synergistic efficiency between enzymes; thiamine, as the key hormone of pyruvate dehydrogenase complex, has a more suitable thiazole ring structure for the reducing environment of the anaerobic system, which can effectively promote the conversion of pyruvate to acetyl-CoA in the hydrolysis acidification stage and improve the sewage treatment efficiency of anaerobic organisms; ammonium acetate can be used as a carbon source and a nitrogen source, and is more easily utilized by anaerobic microorganisms, which can significantly improve the biological activity of anaerobic organisms; inorganic salts maintain osmotic pressure balance and ionic strength, provide buffering action, and provide a suitable living environment for anaerobic microorganisms.

[0087] In summary, the replacement or proportion adjustment of any single component will break the dynamic balance of the system; changing the slow-release carrier ratio and treatment conditions will affect the formation of chitosan-rhamnolipid complexes and the overall effect; and the replacement of coenzymes or salt components will directly interfere with the metabolic pathway. This high degree of synergy indicates that the technical solution of the present application maximizes the degradation efficiency through precise regulation; the present application not only improves the sewage treatment efficiency of anaerobic microorganisms, but also constructs a microenvironment suitable for the metabolic characteristics of anaerobic bacterial flora, and prolongs the action time through slow release, so as to achieve the purpose of promoting the sewage treatment effect of anaerobic organisms.

[0088] (2) In the test conditions of (1), the temperature variable group of Example 1 was selected: the reactor temperature was set to 15℃, 25℃, 45℃ and 55℃ respectively; the target temperature was maintained by a programmable constant temperature water bath jacket (temperature control accuracy ±0.3℃), and the temperature fluctuation was recorded in real time; the strain and the addition method of the growth promoter, the stirring speed and other parameters were consistent with the original test, the SS was measured before and after treatment and the degradation rate was calculated, the influence of temperature on the activity of microorganisms and the efficiency of the growth promoter was analyzed, and the results are shown in Table 3.

[0089] Table 3

[0090] Temperature (°C) Degradation rate (%) of control Degradation rate (%) of example 15 41.0 42.5 25 65 85 35 69.40 95.50 45 63.4 87.6 55 51.2 63.2

[0091] According to Table 3, in the range of 15-55℃, the degradation efficiency of the growth promoter system presents a unimodal curve with the increase of temperature, the peak value appears at 35℃, and the synergistic effect of the growth promoter and the mesophilic anaerobic bacteria group reaches the optimal state. In low temperature environment, the degradation efficiency of the growth promoter is slightly different from that of the control group, it is speculated that the low temperature leads to the change of enzyme protein conformation and the decrease of cell membrane lipid fluidity, which seriously limits the dispersion efficiency of the slow-release carrier and the slow-release kinetics of the active components; in the mesophilic temperature range of 25-45℃, the degradation efficiency of the growth promoter system is always significantly higher than that of the control group, especially at 45℃, it still maintains a high removal rate of 87.6%, which shows that the slow-release carrier forms an amphiphilic molecular layer to stabilize the microbial membrane structure, and the thermal stability of the microcapsule system effectively alleviates the peroxidation damage caused by high temperature, thereby enhancing the tolerance of the bacterial group to thermal stress. The growth promoter in the range of 30-40℃ not only exhibits the optimal degradation efficiency, but also further improves the adaptability of the anaerobic bacteria group to environmental temperature fluctuations by regulating the ionic strength and the stability of metabolic coenzymes.

[0092] (3) In the test conditions of (1), the pH variable group of Example 1 was selected: the pH value of the sewage was set to 5, 6, 8 and 9 respectively; the target pH value was dynamically adjusted by a pH automatic control system with 1 mol / L HCl / NaOH solution; the strain and the addition method of the growth promoter, the stirring speed and other parameters were consistent with the original test, the SS was measured before and after treatment and the degradation rate was calculated, the influence of pH on the adaptability of the growth promoter was explored, and the results are shown in Table 4.

[0093] Table 4

[0094] pH value Degradation rate (%) of control Degradation rate (%) of example 5.0 32.1 40.2 6.0 65.4 85.3 7.0 69.40 95.50 8.0 61.2 91.5 9.0 24.5 33.4

[0095] Table 4 shows that the degradation efficiency of the growth promoter system exhibits a single-peak curve within the pH range of 5.0-9.0, reaching its peak under neutral conditions, which is 26.1% higher than the control group. This indicates that the metabolic synergy between the growth promoter and anaerobic bacteria is optimal at this pH level. In the acidic range, the degradation efficiency of the growth promoter is significantly higher than that of the control group. This is mainly due to the dual protection of the microbial membrane provided by the micellarization effect of the sustained-release carrier: firstly, the micelles adsorb H+... + First, it creates a local buffer microenvironment, mitigating the toxicity of protons to cell membranes. Second, the release of ammonium acetate ions dynamically neutralizes the acidity of the liquid phase, while ferrous sulfate and potassium dihydrogen phosphate form a buffer system, maintaining the metabolic activity of anaerobic microorganisms. Under alkaline conditions, the growth promoter still exhibits high degradation efficiency, the mechanism of which involves the deprotonation behavior of the microencapsulation system: chitosan exhibits enhanced carboxyl dissociation in a weakly alkaline environment, adsorbing heavy metal ions and stabilizing the sludge floc structure through electrostatic interactions, while the thiazole ring of thiamine coenzyme shows superior antioxidant properties under alkaline conditions compared to traditional systems, slowing down the coenzyme degradation rate. Through the synergistic design of physicochemical protection and metabolic regulation, this growth promoter not only significantly enhances the activity of anaerobic bacteria under neutral to weakly acidic / weakly alkaline conditions but also endows the system with tolerance to short-term pH fluctuations.

[0096] (4) Under the basic test conditions in (1), Example 1 and the comparative example were selected for long-term stability operation test. The bacterial strain and growth promoter addition method, stirring rate and other parameters were the same as the original test. The periodic treatment was carried out for 20 consecutive days (each 5 days was one operation cycle). At the end of each cycle, the SS degradation rate was measured and calculated. The sludge dehydrogenase activity (TTC method) was monitored simultaneously. The slow release effect of the growth promoter under long-term operation conditions was analyzed. The results are shown in Table 5.

[0097] Table 5

[0098]

[0099] Table 5 shows that the slow-release system constructed by the present invention exhibits significant sustained performance in long-term stability testing. The SS degradation rate in Example 1 only decreased slightly with increasing operating days, remaining consistently at a high level, indicating that the slow-release carrier effectively ensures a sustained supply of microbial active substances, continuously improving the wastewater treatment effect of anaerobic microorganisms. In contrast, the initial degradation rate of the comparative example was lower than that of the example and fluctuated significantly, reflecting the deficiency of insufficient supply of active substances over time with the conventional addition method without the slow-release carrier. Although the control group showed natural adaptive improvement with operating time, its absolute efficiency still significantly lagged behind the slow-release system. The trend of dehydrogenase activity changes monitored simultaneously highly matched the degradation rate, confirming that the present invention effectively extended the action time of active substances through slow-release design.

[0100] The raw materials and equipment used in the present application are conventional raw materials and equipment in the art unless otherwise specified; the methods used in the present application are conventional methods in the art unless otherwise specified. The above is only a preferred embodiment of the present application, and does not limit the present application in any way. Any simple modification, change and equivalent transformation of the above embodiment based on the technical essence of the present application are still within the protection scope of the technical solution of the present application.

Claims

1. An anaerobic organism growth promoter, characterized by comprising: The bio-promoting agent comprises the following components by weight: enzyme 4-6 parts; exogenous regulatory hormone 0.5-1 part; organic salt 1-2 parts; inorganic salt 0.25-0.75 parts; slow-release carrier 2-3 parts; the slow-release carrier forms microcapsules through the three-dimensional network structure of chitosan molecules, and a dense molecular layer of rhamnolipid is formed on the surface of the microcapsules to construct a slow-release microcapsule structure.

2. The anaerobic organism growth promoter according to claim 1, characterized by, The enzyme is cellulase, hydrogenase and protease, and the weight ratio of cellulase, hydrogenase and protease is 3:1:

1.

3. The anaerobic biostimulant of claim 1, wherein, The exogenous regulatory hormone is one or more of cobalamin, thiamine or hematin; the organic salt is one or more of ammonium formate, ammonium acetate, calcium acetate or calcium gluconate.

4. The anaerobic biostimulant of claim 1, wherein, The inorganic salt comprises sulfate and phosphate, and the weight ratio of sulfate and phosphate is 2-4:

1.

5. The anaerobic biostimulant according to claim 4, characterized in that, The sulfate is one or more of ferrous sulfate, magnesium sulfate or sodium sulfate; the phosphate is one or more of potassium dihydrogen phosphate or sodium dihydrogen phosphate.

6. The anaerobic biostimulant of claim 1, wherein, In the slow-release carrier, the weight ratio of rhamnolipid to chitosan is 1:1-2.

7. A method for producing the anaerobic microorganism growth promoter as claimed in any one of claims 1 to 6, characterized by, The method comprises the following steps: adding the slow-release carrier into water and mixing, and then adding inorganic salt, exogenous regulatory hormone, organic salt and enzyme into the solution and mixing to obtain the bio-promoting agent.

8. A method for preparing an anaerobic biological growth promoter according to claim 7, characterized in that, After the slow-release carrier is added into water, ultrasonic treatment is used for uniform mixing; the ultrasonic treatment power is 200-400 W, the temperature is 20-30 DEG C, and the time is 30-60 min.

9. The method for preparing an anaerobic biological growth promoter according to claim 7, characterized in that, In the adding step, the inorganic salt, exogenous regulatory hormone, organic salt and enzyme are sequentially added in order, and the mixing and stirring time is 20-30 min.

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