Neural stem cell peptide for treating Alzheimer's disease and preparation method and application thereof

By inducing iPS cells to differentiate into neural stem cells in stages, combining specific processing techniques to enrich small molecule active peptides, the problems of low differentiation efficiency of iPS cells and traditional NSC sources are solved, and the effective inhibition of β-amyloid deposition is achieved, providing better Alzheimer's disease treatment.

CN120248011APending Publication Date: 2025-07-04FUMEI ZHONGKANG MEDICAL TECHNOLOGY (ZHUHAI HENGQIN) CO LTD
0 Cites 0 Cited by

Patent Information

Application Number
CN202510416752.4
Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
Filing Date
2025-04-03
Publication Date
2025-07-04

AI Technical Summary

Technical Problem

In the prior art, iPS cells have low differentiation efficiency to neural stem cells, the concentration of neurotrophic factors in the secretions is unstable, and it is difficult to play an efficient role through the blood-brain barrier. There are ethical controversy among traditional NSC sources and low amplification efficiency.

Method used

The differentiation of iPS cells into neural stem cells was carried out in stages, and the neural stem cells were treated with specific composition medium and lysis buffer. The nanomolecule, centrifugation, ultrafiltration and ion exchange chromatography were combined to enrich small-molecule active peptides.

Benefits of technology

Obtaining a high-purity small-molecule active peptide can effectively inhibit β-amyloid deposition, provide better treatment effects for Alzheimer's disease, and enhance the ability to regulate key pathology of AD.

✦ Generated by Eureka AI based on patent content.
Patent Text Reader

Abstract

The invention relates to the technical field of stem cell biology. The invention provides a neural stem cell peptide for treating Alzheimer's disease and a preparation method thereof, and the preparation method comprises the following steps: (1) inoculating an iPS cell to a culture medium A, transferring to a culture medium B after an embryo body is formed, and transferring to a culture medium C for continuous culture after induction culture to the 15th-20th day to obtain a neural stem cell; (2) placing the neural stem cells in a lysis buffer solution, and performing ultrasonic treatment and centrifugation to obtain supernate; and (3) carrying out ultrafiltration and ion exchange chromatography on the supernate to obtain the product. According to the technical scheme, the small-molecule active peptide can be efficiently enriched, the obtained neural stem cell peptide can effectively inhibit beta-amyloid protein deposition, the Alzheimer's disease can be better treated, and the neural stem cell peptide has a good application prospect in the field of biomedicine.
Need to check novelty before this filing date? Find Prior Art