Cichorium intybus pectin extract with skin care effect as well as preparation method and application of cichorium intybus pectin extract

Abstract

The invention belongs to the technical field of plant extracts, and discloses a cichorium intybus pectin extract with a skin care effect as well as a preparation method and application of the cichorium intybus pectin extract. The preparation method comprises the following steps: taking inulin, mixing with a citric acid solution, heating, extracting, cooling, filtering to obtain filtrate, carrying out centrifugal separation on the filtrate to obtain supernate, then carrying out alcohol precipitation, then carrying out centrifugal separation to obtain flocculate, washing, and drying to obtain the endive pectin extract. The cichorium intybus pectin extract prepared by the preparation method is high in yield, and has a better effect in the aspect of resisting skin aging or inflammation compared with the existing citrus pectin or apple pectin.

Description

Technical Field

[0001] The present invention belongs to the technical field of plant extracts, and particularly relates to a chicory pectin extract with skin care efficacy, a preparation method thereof, and an application thereof. Background Art

[0002] In the field of skin care, some commonly used synthetic compounds have certain side effects and may have relatively high synthesis costs. Natural plants have fewer side effects and are more beneficial to the human body. Therefore, in the prior art, attempts have been made to use natural plants as the main raw material to obtain active ingredients, so as to achieve better skin care effects.

[0003] For example, citrus pectin or apple pectin extracted from plants has certain skin care effects, but the effects need to be further improved.

[0004] Therefore, there is an urgent need to provide a plant extract with better skin care effects. Summary of the Invention

[0005] The present invention aims to solve at least one of the technical problems existing in the above prior art. For this purpose, the present invention provides a chicory pectin extract with skin care efficacy, a preparation method thereof, and an application thereof. The chicory pectin extract obtained by the preparation method of the present invention has unique effects in promoting the establishment of skin barriers and anti-skin aging.

[0006] The first aspect of the present invention provides a preparation method of a chicory pectin extract with skin care efficacy.

[0007] A preparation method of a chicory pectin extract with skin care efficacy includes the following steps:

[0008] Take inulin and mix it with a citric acid solution, heat for extraction, cool, filter to obtain a filtrate, subject the filtrate to centrifugal separation to obtain a supernatant, then perform alcohol precipitation, and then perform centrifugal separation to obtain a flocculent substance, wash, and dry to obtain the chicory pectin extract.

[0009] Preferably, the inulin includes chicory root powder.

[0010] Preferably, the dosage ratio of the inulin to the citric acid solution is 500 g:(3 - 8) L, and more preferably 500 g:(4 - 6) L.

[0011] Preferably, the mass fraction of the citric acid solution is 0.1 - 20%, and more preferably 1 - 15%.

[0012] Preferably, the pH of the citric acid solution is 1 - 5, more preferably 2 - 4, and even more preferably 2.

[0013] Preferably, the temperature for heating extraction is 70 - 90 °C, and the time for heating extraction is 1 - 5 h; more preferably, the temperature for heating extraction is 80 °C, and the time for heating extraction is 4 h.

[0014] Preferably, the filtration is carried out using a filter cloth with 100 - 200 meshes.

[0015] Preferably, during the heating extraction process, stirring is also carried out, and the stirring rate is 20 - 50 r / min.

[0016] Preferably, during the process of obtaining the flocculant, the rotational speed for centrifugal separation is 8000 - 10000 r / min, and the time for centrifugal separation is 10 - 20 min.

[0017] Preferably, during the alcohol precipitation process, the supernatant is added to 95% by volume of ethanol with a volume 1 - 3 times that of the supernatant, and after sufficient stirring, alcohol precipitation is carried out for 2 - 3 h.

[0018] Preferably, during the washing process, the flocculant is washed twice with ethanol with a volume fraction of 75% and 95% respectively, and centrifuged under the conditions of a rotational speed of 8000 - 10000 r / min and a centrifugal separation time of 10 - 20 min.

[0019] Preferably, the drying is to place the washed flocculant in a drying oven at 40 - 45 °C until constant weight, and then weigh it with an analytical balance.

[0020] The second aspect of the present invention provides a chicory pectin extract with skin care efficacy.

[0021] A chicory pectin extract with skin care efficacy is prepared by the above preparation method.

[0022] The third aspect of the present invention provides an application of a chicory pectin extract with skin care efficacy.

[0023] The above application of chicory pectin extraction in the preparation of anti - skin aging or anti - inflammatory drugs.

[0024] Compared with the prior art, the beneficial effects of the present invention are as follows:

[0025] The chicory pectin extract prepared by the specific preparation method of the present invention (mixing inulin with a citric acid solution, heating extraction, cooling, filtration to obtain a filtrate, centrifuging the filtrate to obtain a supernatant, then carrying out alcohol precipitation, and then centrifuging again to obtain a flocculant, and washing) has a high yield, and in terms of anti - skin aging or anti - inflammation, it has better effects compared with the existing citrus pectin or apple pectin. Description of the Drawings

[0026] Figure 1It is the determination curve of galacturonic acid content;

[0027] Figure 2 It is the environmental scanning electron microscope results of chicory pectin extract at different magnifications;

[0028] Figure 3 It is the environmental scanning electron microscope results of citrus pectin at different magnifications;

[0029] Figure 4 It is the environmental scanning electron microscope results of apple pectin at different magnifications;

[0030] Figure 5 It is the inhibition rate of chicory pectin extract on hyaluronidase at different concentrations;

[0031] Figure 6 It is the result of the inhibition rate of chicory pectin extract on metalloproteinase;

[0032] Figure 7 It is the result of the inhibition rate of chicory pectin extract on elastase;

[0033] Figure 8 It is the protein bands of HAS2, β-actin and AQP3 in HaCaT cells;

[0034] Figure 9 It is the protein bands of β-actin and Casp-14 in HaCaT cells;

[0035] Figure 10 It is the relative ratio of HAS2 protein in HaCaT cells;

[0036] Figure 11 It is the relative ratio of AQP3 protein in HaCaT cells;

[0037] Figure 12 It is the relative ratio of Casp14 protein in HaCaT cells. Detailed implementation manners

[0038] In order to make the technical solutions described in the present invention clearer and more understandable to those skilled in the art, the following examples are listed for illustration. It should be noted that the following examples do not limit the scope of protection required by the present invention.

[0039] The raw materials, reagents or devices used in the following examples can be obtained from conventional commercial channels or can be obtained by existing known methods without special instructions.

[0040] Example 1

[0041] A preparation method of a chicory pectin extract with skin care efficacy, comprising the following steps:

[0042] Weigh 500.0 g of inulin dry meal powder (chicory root powder), put it into a 10 L round-bottom flask, add 5 L of citric acid solution (pH of the citric acid solution = 2), extract in hot water at 80 °C for 4 h at a rotation speed of 25 r / min. After the extraction is completed, immediately cool it to room temperature with ice water, and filter the material liquid through a 100-mesh filter cloth to obtain a filtrate;

[0043] Pour the filtrate into a centrifuge tube, centrifuge at a rotation speed of 10000 r / min for 20 min, take the supernatant, slowly add the supernatant to 3 times the volume of 95% volume fraction ethanol, stir well and then carry out alcohol precipitation for 3 h. Subsequently, centrifuge at a rotation speed of 8000 r / min for 10 min to obtain a flocculent substance. Wash the flocculent substance twice with 75% volume fraction and 95% volume fraction ethanol respectively, and centrifuge at a rotation speed of 8000 r / min for 10 min, collect the flocculent substance, and place it in a drying oven at 45 °C to dry to a constant weight to obtain chicory pectin extract (abbreviated as chicory pectin).

[0044] In this example, about 45.38 g of chicory pectin extract is obtained, and the yield is 9.076%.

[0045] Product effect test

[0046] 1. Physicochemical properties of chicory pectin extract

[0047] Determination of galacturonic acid content.

[0048] The experimental materials are as follows:

[0049] Carbazole ethanol; sulfuric acid; water bath; ice water; microplate reader.

[0050] Experimental method

[0051] Refer to the spectrophotometric method for the determination of pectin content in NY / T2016 - 2011 "Determination of Pectin Content in Fruits and Their Products" to determine the pectin content. Prepare a pectin solution with an appropriate concentration, accurately pipette 1.0 mL of the pectin solution into a 10 mL test tube, add 0.25 mL of carbazole ethanol solution, mix well and then quickly add 5.0 mL of sulfuric acid and shake well. Place the test tube in a water bath at 85 °C and shake for 20 min, take it out and quickly cool it to room temperature in ice water. Within 1.5 h, measure the absorbance of the solution at a wavelength of 525 nm with a spectrophotometer. Calculate the content of galacturonic acid in pectin according to the galacturonic acid standard curve, and the galacturonic acid standard curve is as Figure 1 shown.

[0052] The experimental results are shown in Table 1.

[0053] Table 1: Results of the determination of lacturonic acid content

[0054]

[0055] It can be seen that the galacturonic acid content in the chicory pectin extract of Example 1 of the present invention is 46.54%.

[0056] 2. Determination of Esterification Degree

[0057] The experimental materials are as follows:

[0058] Chicory pectin powder; absolute ethanol; magnetic thermostatic stirrer; phenolphthalein; NaOH (0.1 mol / L); HCl (0.1 mol / L); burette for alkaline titration.

[0059] Experimental method:

[0060] The titration method was used for determination. Weigh 1 g of the chicory pectin extract of Example 1, moisten it with a small amount of absolute ethanol, add 50 mL of distilled water, place it on a magnetic thermostatic stirrer and stir until dissolved. Add 1 drop of phenolphthalein, and titrate with NaOH (0.1 mol / L) until it just changes color and record the consumed volume (V1). Then continue to add 10 mL of NaOH (0.1 mol / L), mix well, let it stand for 2.0 h (at room temperature), add 10 mL of HCl (0.1 mol / L), and finally titrate with NaOH (0.1 mol / L) until it just changes color and record the consumed volume (V2). Calculate the esterification degree of pectin according to the formula:

[0061]

[0062]

[0063] The experimental results are as follows:

[0064] V1 = 4.00 mL; V2 = 9.20 mL; esterification degree = 69.69%; methoxy content = 11.36%.

[0065] 3. Environmental Scanning Electron Microscopy Analysis

[0066] The experimental materials are as follows:

[0067] FEI Quanta 200 environmental scanning electron microscope, chicory pectin extract of Example 1, citrus pectin, apple pectin.

[0068] The experimental method is as follows:

[0069] Fix the chicory pectin extract, citrus pectin, and apple pectin powders on the stage with conductive double-sided tape respectively. After the samples are gold-plated, observe their surface morphologies using a FEI Quanta 200 environmental scanning electron microscope.

[0070] The experimental results are as Figure 2 、 Figure 3 、 Figure 4 shown.

[0071] FromFigure 2 , Figure 3 , Figure 4 It can be seen that the chicory pectin extract has larger particles and more regular shapes at 100× (magnified 100 times); the surface of the chicory pectin extract is smooth at 500×, and the sectional lines are obvious. At 1500 - 10000×, the section shows a laminated morphology. The powders of citrus pectin and apple pectin have smaller particles and scattered and irregular shapes at 100× and 200×; at 1200×, the citrus pectin and apple pectin are uneven and show a laminated morphology. At 5000 - 10000×, there are still fine particles, and there are a large number of irregular depressions on the surface of the main powder particles.

[0072] It can be known from this that compared with citrus pectin and apple pectin, the chicory pectin extract is arranged in an orderly manner, with fewer surface defects, steps, and dislocations, and lower surface energy. Its chemical activity is relatively weak, and the reaction rate may be slower. The dissolution rate is relatively uniform, and the diffusion path is clear. It has high stability, long lifespan, good fluidity, and controllable release. When used as a skincare ingredient, it can reduce the use of stabilizers and bacteriostatic agents.

[0073] 4. Test on the inhibitory effects on hyaluronidase, metalloproteinase, and elastase

[0074] 4.1 Hyaluronidase inhibition test

[0075] The experimental materials are as follows:

[0076] Hyaluronidase: concentration 1500 U / mL (300 U / mg, 5 mg / mL), freshly prepared and used immediately, not overnight, with acetate buffer as the solvent;

[0077] Sodium hyaluronate: 8 mg / mL, prepared once and used multiple times, with acetate buffer as the solvent;

[0078] Buffer:

[0079] Solution A (2.4 mL of 0.2 mol / L acetic acid, 57.75 μL of glacial acetic acid dissolved in 5 mL of distilled water);

[0080] Solution B (22.6 mL of 0.2 mol / L sodium acetate, 0.68 g of sodium acetate trihydrate dissolved in 25 mL of distilled water), mixed and diluted to 50 mL to prepare an acetate buffer with pH = 5.6;

[0081] Acetylacetone solution: 25 mL of 1.0 mol / L sodium carbonate solution and 1.75 mL of acetylacetone solution mixed evenly (prepared immediately before use);

[0082] P-DAB chromogenic reagent: 0.8 g of p-dimethylaminobenzaldehyde dissolved in a mixture of 15 mL of concentrated hydrochloric acid and 15 mL of absolute ethanol and mixed evenly;

[0083] Calcium chloride solution CaCl2: 2.5 mol / L;

[0084] Sodium hydroxide solution NaOH: 5 mol / L;

[0085] Samples: Chicory pectin extract from Example 1 prepared at different concentrations: 1.5 mg / mL; 3 mg / mL; 6 mg / mL; 9 mg / mL; 12 mg / mL;

[0086] Experimental method:

[0087] Prepare four 15 mL centrifuge tubes labeled as tubes A, B, C, and D. Tube A: 0.5 mL of sample, 0.5 mL of hyaluronidase; Tube B: 0.5 mL of sample, 0.5 mL of acetate buffer; Tube C: 0.5 mL of distilled water, 0.5 mL of hyaluronidase; Tube D: 0.5 mL of distilled water, 0.5 mL of acetate buffer; Place the four centrifuge tubes in a 37 °C water bath and incubate for 20 min.

[0088] After taking out, add 0.1 mL of CaCl2 solution to each of the four centrifuge tubes; place the four centrifuge tubes in a 37 °C water bath again and incubate for 20 min; add 0.5 mL of sodium hyaluronate to Tube A, 0.5 mL of acetate buffer to Tube B, 0.5 mL of sodium hyaluronate to Tube C, and 0.5 mL of acetate buffer to Tube D; place the four centrifuge tubes in a 37 °C water bath and incubate for 40 min, then take out and let stand at room temperature for 10 min.

[0089] Add 0.5 mL of distilled water, 0.1 mL of NaOH solution, and 0.5 mL of acetylacetone solution to each of the four centrifuge tubes; place the four centrifuge tubes in a boiling water bath for 15 min, ice bath for 10 min, and let stand at room temperature for 10 min; after taking out, add 1 mL of P-DAB color reagent to each of the four centrifuge tubes; after shaking each tube well, let stand at room temperature for 30 min and then measure the absorbance at 530 nm.

[0090] The experimental results are as follows:

[0091] When the concentration of chicory pectin extract is 3 mg / ml, the inhibition rate of hyaluronidase can reach 20.30%.

[0092] 4.2 Matrix metalloproteinase inhibition test

[0093] The experimental materials are as follows:

[0094] 50 mmol / L boric acid buffer; 1600 U / mL collagenase IV (160 U / mg) solution (the solvent is boric acid buffer); 50 mg / mL succinylated gelatin solution (the solvent is boric acid buffer); 0.03% 2,4,6-trinitrobenzenesulfonic acid (TNBSA); Chicory pectin extract solution from Example 1 (abbreviated as pectin solution, the solvent is boric acid buffer).

[0095] The experimental method is as follows:

[0096] On a 96-well cell culture plate, add 10 μL of collagenase IV solution, 100 μL of succinylated gelatin solution, and 40 μL of pectin solution to each well. The concentrations of the pectin solution are 3.75 mg / mL, 7.5 mg / mL, 11.25 mg / mL, 15 mg / mL, and 18.75 mg / mL respectively. After addition, the final concentrations of the pectin solution are 1 mg / mL, 2 mg / mL, 3 mg / mL, 4 mg / mL, and 5 mg / mL. Incubate at 37 °C for 30 min; add 50 μL of 0.03% TNBSA and let it stand at room temperature for 20 min, then measure the absorbance at 450 nm. Calculate the inhibition rate according to the equation I% = [(1 - Ai / A0) × 100]%; where: Ai represents the absorbance value after adding the inhibitor; A0 represents the absorbance value of the blank.

[0097] The experimental results are as Figure 6 shown.

[0098] It can be seen from Figure 6 that the inhibition rate of chicory pectin extract on metalloproteinase is linearly correlated when the pectin concentration is 0 - 4 mg / mL. When the pectin concentration is greater than 4 mg / mL, the growth rate of the metalloproteinase inhibition rate decreases, and with the increase of the pectin concentration, the growth of the metalloproteinase inhibition rate is not obvious.

[0099] When the concentration of chicory pectin is 3 mg / mL, the inhibition rate of metalloproteinase has reached 50%. When the pectin concentration increases from 4 mg / mL to 5 mg / mL, the inhibition rate of metalloproteinase only increases by 2.07%.

[0100] 4.3 Elastase inhibition test

[0101] The experimental materials are as follows:

[0102] 0.05 mol / L Tris-HCl buffer solution with pH = 7.6; 0.025 mg / mL elastase (30 U / mg) solution (the solvent is Tris-HCl buffer solution); 0.25 mg / mL N-succinyl-alanine-alanine-alanine-p-nitroaniline solution (the solvent is Tris-HCl buffer solution); the chicory pectin extract solution of Example 1 (the solvent is Tris-HCl buffer solution).

[0103] The experimental method is as follows:

[0104] When measuring, take 25 μL of 0.025 mg / mL elastase solution, mix it with 50 μL of pectin solutions at 2 mg / mL, 4 mg / mL, and 8 mg / mL respectively in a 96-well plate. After 5 min, add 25 μL of 0.25 mg / mL N-succinyl-alanine-alanine-alanine-p-nitroaniline solution, and then place it in an enzyme-linked immunosorbent assay (ELISA) reader to measure the absorbance at a wavelength of 405 nm.

[0105] The experimental results are as Figure 7 shown.

[0106] It can be seen from Figure 7 that the inhibition rates of the chicory pectin extract in Example 1 on elastase at low concentration (2 mg / mL), medium concentration (4 mg / mL), and high concentration (8 mg / mL) are 3%, 27.12%, and 33.82% respectively, and the inhibition rate of boswellin on elastase at all concentrations is not higher than 3%.

[0107] It can be seen that the chicory pectin extract has inhibitory effects on hyaluronidase, matrix metalloproteinase, and elastase. Among them, the inhibitory effect on matrix metalloproteinase is stronger. Matrix metalloproteinase participates in multiple signaling pathways in skin cells and is closely related to cell differentiation, proliferation, and inflammation. It can degrade collagen and elastin in the dermis. Based on its important role in the process of skin aging, people gradually regard inhibiting the activity of matrix metalloproteinase, inhibiting abnormal collagen degradation, reducing skin inflammatory reactions, and preventing skin photoaging as new ways to delay aging. Therefore, the chicory pectin extract has potential efficacy in preventing skin aging.

[0108] 4.4 Effects of Chicory Pectin Extract on the Expression of Related Proteins in Human Immortalized Keratinocytes

[0109] The experimental materials are as follows:

[0110] PAGE (polyacrylamide) gel rapid preparation kit; strong RIPA lysis buffer (radioimmunoprecipitation assay lysis buffer); protease inhibitor PMSF (phenylmethylsulfonyl fluoride); BCA (protein concentration determination) kit; Tris (tris(hydroxymethyl)aminomethane); glycine; SDS (sodium dodecyl sulfate); sodium chloride; Loading Buffer (loading buffer); rapid transfer buffer; rapid blocking solution; HAS2 recombinant antibody; CASP14 polyclonal antibody; Anti-Aquaporin 3 antibody; β-actin (internal reference); Goat Anti-Rabbit Mouse (secondary antibody).

[0111] The experimental methods are as follows:

[0112] Protein extraction: Refer to the conventional Western blot method

[0113] Protein content determination: Mix reagent A and reagent B in a volume ratio of 50:1 to prepare a BCA working solution. Completely dissolve the BSA (bovine serum albumin) protein standard solution at room temperature. Dilute the BSA protein standard solution with PBS solution (phosphate buffer solution) to make its final concentrations 25 μg / mL, 125 μg / mL, 250 μg / mL, 500 μg / mL, 750 μg / mL, 1000 μg / mL, 1500 μg / mL, and 2000 μg / mL. Add 20 μL of the sample to be tested into the microplate. Add 200 μL of the BCA working solution into the microplate, mix well, and incubate at 37 °C for 30 min. Measure the absorbance at 562 nm and record the reading; use the light absorption value of the sample without BCA as the blank control. Plot the standard curve and calculate the protein concentration. If the protein concentration is not within the range of the standard curve, dilute the sample and measure again.

[0114] Gel preparation: Refer to the conventional Western blot method

[0115] Western blot running gel parameters: Constant voltage of 65 V. After electrophoresis for about 30 minutes, when the marker is separated, change the voltage to 110 V, and then perform constant voltage electrophoresis for about 60 minutes. Constant current of 200 mA in an ice box, transfer the membrane for 90 minutes.

[0116] After 48 h of administration of chicory pectin (the chicory pectin extract of Example 1), lyse the HaCaT cells with a powerful RIPA lysis buffer (a rapid cell tissue lysis buffer) supplemented with the protease inhibitor PMSF (phenylmethylsulfonyl fluoride). The collected protein is denatured at 100 °C for 5 minutes. The samples are separated by 10% SDS-polyacrylamide gel electrophoresis, cut the bands according to the molecular size of the detected protein with reference to the marker, and transfer them to an activated PVDF (polyvinylidene fluoride) membrane. Block the transferred membrane in a rapid blocking solution for 40 min, and incubate at 4 °C for 16 h in buffers containing primary antibodies against β-actin, HAS2, AQP3, and CASP14 respectively. After incubation, wash the membrane 3 times with 1×TBST, 10 min each time, and treat it with a goat anti-rabbit / mouse IgG secondary antibody for 2 h. Finally, wash the membrane 3 times with 1×TBST again, 10 min each time. Detect the signal using an ECL kit and visualize it with a Tianneng imager 5200Multi. Quantify the size of each band using Image J and normalize it to the β-actin band.

[0117] The experimental results are as Figures 8 to 12 shown.

[0118] The administration concentrations of chicory pectin were 2 mg / mL (low dose), 4 mg / mL (medium dose), and 8 mg / mL (high dose) respectively, the administration concentration of citrus pectin was 4 mg / mL, the administration concentration of boswellic acid was 10 μg / mL, and the administration concentration of resveratrol was 30 μg / mL. The protein loading amount for each group was 30 μg.

[0119] The administration concentrations of chicory pectin were 2 mg / mL, 4 mg / mL, and 8 mg / mL respectively, and the administration concentration of citrus pectin was 4 mg / mL. The protein loading amount for each group was 20 μg.

[0120] As the administration concentration of chicory pectin increased, the inhibition rate of HAS2 protein slightly increased. At the medium concentration (4 mg / mL) of chicory pectin, the inhibition rate of HAS2 protein was slightly lower than that of citrus pectin and resveratrol, and slightly higher than that of boswellic acid. As the concentration increased, the inhibition rate of chicory pectin on AQP3 protein increased significantly. At the medium concentration (4 mg / mL), the inhibition rate of AQP3 protein was slightly lower than that of citrus pectin, boswellic acid, and resveratrol. At the high concentration (8 mg / mL), it was slightly higher than that of citrus pectin, boswellic acid, and resveratrol.

[0121] It can be seen that the chicory pectin extract in Example 1 had no significant effect on promoting the expression of HAS2 and AQP3 in HaCaT cells, but the effects at medium and high concentrations were still better than those of citrus pectin at the same concentration. In promoting the expression of Casp14, the chicory pectin extract had a significant effect. The expression level after administration at the medium concentration increased to more than 200%, far higher than that of citrus pectin at the same concentration. Casp14 is a member of the Caspase family and is mainly distributed in the epidermis of the human body and is rarely seen in other tissues. The expression of Casp14 is mainly concentrated in the cells participating in the formation of barrier tissues to form epidermal barrier tissues. Combining with the experimental results of inhibiting metalloproteinases, the chicory pectin extract has a unique effect in promoting skin barrier establishment and anti-skin aging.

Claims

1. A method for preparing chicory pectin extract, characterized in that, It includes the following steps: Mix inulin with a citric acid solution, heat for extraction, cool, filter to obtain a filtrate, subject the filtrate to centrifugal separation to obtain a supernatant, then perform alcohol precipitation, followed by centrifugal separation to obtain a flocculant, wash, and dry to obtain the chicory pectin extract.

2. The preparation method according to claim 1, wherein, The inulin includes chicory root powder.

3. The preparation method according to claim 1, characterized in that, The dosage ratio of the inulin to the citric acid solution is 500 g : (3 - 8) L.

4. The preparation method according to claim 1, characterized in that, The pH of the citric acid solution is 1 - 5.

5. The preparation method according to any one of claims 1-4, characterized in that, The temperature for the heating extraction is 70 - 90 °C, and the time for the heating extraction is 1 - 5 h.

6. The preparation method according to claim 1, characterized in that, During the process of obtaining the flocculant, the rotation speed for centrifugal separation is 8000 - 10000 r / min, and the time for centrifugal separation is 10 - 20 min.

7. The preparation method according to claim 1, wherein During the alcohol precipitation process, the supernatant is added to 95% by volume of ethanol with a volume 1 - 3 times that of the supernatant, and after sufficient stirring, alcohol precipitation is carried out for 2 - 3 h.

8. The preparation method according to claim 1, wherein, The washing process is to wash the flocculant twice with 75% and 95% by volume of ethanol respectively, and the rotation speed is 8000 - 10000 r / min, and the time for centrifugal separation is 10 - 20 min.

9. A chicory pectin extract, characterized in that, Prepared by the preparation method according to any one of claims 1 - 8.

10. Use of the chicory pectin extract prepared by the preparation method according to any one of claims 1 - 8 in the preparation of anti - skin - aging or anti - inflammatory drugs.

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