Medical examination microorganism culture equipment and culture method

Abstract

The invention discloses medical examination microorganism culture equipment and a culture method, and relates to the technical field of microorganism culture, the medical examination microorganism culture equipment comprises a cabinet body, a constant-temperature box is fixedly connected in the cabinet body, a culture box is slidably connected in the constant-temperature box, and an air supply mechanism is connected between the culture box and the constant-temperature box; the air supplementing mechanism is used for supplementing air into the incubator, the adjusting mechanism is arranged in the air supplementing mechanism and used for adjusting the amount of the air supplemented into the incubator by the air supplementing mechanism, and the exhaust mechanism is arranged on the side, away from the air supplementing mechanism, of the incubator and used for intermittently adjusting the density of the air in the incubator. When the culture box slides, the culture box can adjust the air inlet amount of the air supply mechanism through the adjusting mechanism, the exhaust mechanism can be intermittently opened and closed, the air pressure in the culture box is increased, the air in the culture box is exhausted through the exhaust mechanism, and the auxiliary microorganism culture device can facilitate auxiliary microorganism culture.

Description

Technical Field

[0001] The present invention relates to the technical field of microorganism culture, and particularly relates to a medical test microorganism culture device and a culture method. Background Art

[0002] Microorganism culture is to inoculate a sample into one or more culture bottles or culture dishes to discover and identify bacteria or other culturable microorganisms. Detecting microorganisms in a patient's blood has important clinical significance for the diagnosis, treatment, and prognosis of infectious diseases. In medical tests, it is often necessary to culture microorganisms or corresponding bacteria for inspection and observation, so as to carry out immune clinical treatment, medical care and other procedures. Therefore, effective culture of various bacteria and microorganisms in the laboratory department is very important.

[0003] Currently, when culturing microorganisms, it is usually necessary to place the culture bottle or culture dish containing the microorganisms in an incubator and perform shaking or static culture at a specific temperature and humidity to facilitate the microorganisms to better absorb the nutrient solution. Since there are four growth stages in microorganism culture, namely the adjustment period, the logarithmic phase, the stationary phase, and the decline phase, when culturing microorganisms that require shaking-assisted culture, it is usually necessary to supplement pure gas to meet the gas conditions in different growth cycles of the microorganisms. Since microorganism culture has different growth stages and the amount of gas required in different growth stages is also different, the current equipment can only shake to assist in the culture of microorganisms, and cannot adjust the amount of gas required according to the state of different growth cycles of the microorganisms. Moreover, after injecting a large amount of pure gas, and during the rapid growth of the microorganisms, gases such as carbon dioxide can be generated, which causes the gas pressure in the culture equipment to gradually increase. The mixture of the gases generated by the microorganisms themselves and the pure gas is also not conducive to the growth of the microorganisms. And directly connecting the culture equipment to the outside for exhaust is not conducive to the pure gas to better assist the growth of the microorganisms.

[0004] Therefore, an object of the present invention is to provide a medical test microorganism culture device and a culture method to solve the above problems. Summary of the Invention

[0005] The main object of the present invention is to provide a medical test microorganism culture device and a culture method, which can effectively solve the technical problems in the background art.

[0006] To achieve the above object, the technical solution adopted by the present invention is as follows: A medical test microbial culture device includes a cabinet body, a constant temperature box is fixedly connected inside the cabinet body, a culture box is slidably connected inside the constant temperature box, an air supplement mechanism is connected between the culture box and the constant temperature box, the air supplement mechanism is used to supplement gas into the culture box, an adjustment mechanism is arranged inside the air supplement mechanism and is used to adjust the amount of gas supplemented into the culture box by the air supplement mechanism, and an exhaust mechanism is arranged on the side of the culture box away from the air supplement mechanism and is used to intermittently adjust the gas density inside the culture box; When the culture box slides, the culture box can adjust the air intake of the air supplement mechanism through the adjustment mechanism, and enable the exhaust mechanism to open and close intermittently, the gas pressure inside the culture box increases, so that the gas inside the culture box is discharged through the exhaust mechanism.

[0007] Preferably, the air supplement mechanism includes a connection block fixedly connected to the constant temperature box, an air supplement channel is opened inside the connection block, one end of the air supplement channel close to the culture box is communicated with the culture box through a connecting pipe, the other end of the air supplement channel away from the culture box is fixedly connected with an air supplement pipe, and the free end of the air supplement pipe extends out of the constant temperature box and the cabinet body in sequence.

[0008] Preferably, the adjustment mechanism includes a receiving groove opened on the connection block, a rotating ring is rotatably connected inside the receiving groove, one end of the rotating ring close to the connecting pipe is fixedly connected with an adjustment plate, a plurality of arc-shaped grooves are circumferentially and spacedly arranged on the adjustment plate, a plurality of baffles corresponding to the arc-shaped grooves are slidably connected inside the connection block, the plurality of baffles can block the air supplement channel, one end of each baffle away from the air supplement channel is fixedly connected with an insertion rod, and the free end of each insertion rod is inserted into the corresponding arc-shaped groove. When the adjustment plate rotates, the plurality of insertion rods can slide synchronously along the arc-shaped groove towards the direction of the air supplement channel.

[0009] Preferably, an inclined guiding groove is opened on the circumferential side of the rotating ring, a vertically extending guiding rod is slidably connected to the connection block, a top rod is fixedly connected to the side of the guiding rod close to the rotating ring, the top rod is slidably connected with the guiding groove, a pressing block is arranged on the side of the culture box close to the connection block, the side of the pressing block close to the connection block is a wedge-shaped surface, the end of the guiding rod close to the culture box extends out of the connection block and can contact the pressing block, an electric telescopic rod is connected inside the culture box, the output end of the electric telescopic rod is fixedly connected with the pressing block, and the guiding rod and the connection block are connected through a second elastic member.

[0010] Preferably, the exhaust mechanism includes an exhaust groove that is opened on the incubator and runs through it horizontally. A plugging plate is slidably connected in the exhaust groove. A plurality of air outlet grooves are circumferentially and spacedly arranged on the plugging plate. A plurality of plugging blocks corresponding to the air outlet grooves are fixedly connected in the exhaust groove. Each plugging block can extend into the corresponding air outlet groove. A positioning ring is fixedly connected in the exhaust groove. The positioning ring and the plugging plate are connected by a first elastic member.

[0011] Preferably, an auxiliary block is fixedly connected to one side of the incubator close to the exhaust groove. An air release groove communicating with the air outlet groove is opened on the auxiliary block. A slideway communicating with the air release groove is opened on the auxiliary block. An intermittent plate for plugging the air release groove is slidably connected in the slideway. An air guide groove capable of being located in the air release groove is opened on the intermittent plate. One end of the plugging plate away from the incubator can be inserted into the air guide groove.

[0012] Preferably, a piston chamber communicating with the slideway is opened in the auxiliary block. A piston rod is slidably connected in the piston chamber. One end of the piston rod away from the incubator can contact the inner wall of the thermostat. When the piston rod slides, it can drive the intermittent plate to slide.

[0013] Preferably, a supporting block is fixedly connected in the thermostat and located below the incubator. A driving motor is installed on the supporting block. A limiting ring is fixedly connected to one side of the incubator close to the driving motor. A disc is coaxially and fixedly connected to the output end of the driving motor. A push rod is eccentrically fixedly connected to the disc. The push rod extends into the limiting ring. A mass sensor is connected to the incubator. The mass sensor is electrically connected to the driving motor and the electric telescopic rod.

[0014] Preferably, a cabinet door capable of plugging the thermostat is hinged on the cabinet body. A transparent plate capable of plugging the opening of the incubator is hinged at the opening of the incubator. A clamping groove is opened on the inner wall of the incubator.

[0015] A method for culturing medical test microorganisms includes the following steps: S1: First, place the culture dish or culture bottle containing microorganisms in the incubator, and then adjust the thermostat to provide a stable growth environment. S2: Then start the driving motor. The driving motor drives the microorganisms in the incubator to shake, improving the efficiency of microorganism growth. S3: Next, through the shaking of the incubator, the air supplement mechanism supplements gas into the incubator, and the adjustment mechanism can adjust the gas volume according to different growth stages of microorganisms. S4: Finally, through the shaking of the incubator, the exhaust mechanism can be intermittently opened, thereby ensuring the stability of the gas inside the incubator, facilitating the growth of microorganisms. After the cultivation is completed, the petri dish or culture bottle can be taken out.

[0016] Technical effects and advantages of the present invention: 1. According to the quality changes of microorganisms in different growth cycles in the present invention, the quality sensor adjusts the position of the pressing block through the electric telescopic rod, so that the area of the air supplement channel blocked by multiple baffle plates can be adjusted through the pressing block, the guiding rod and the rotating ring, thereby automatically adjusting the air intake of the air supplement channel during different growth cycles of microorganisms, reasonably controlling the air intake, facilitating to meet the gas requirements of microorganisms in different growth cycles, and facilitating the better growth of microorganisms.

[0017] 2. Through the swinging of the incubator in the present invention, the piston rod can be intermittently squeezed, so that the exhaust groove and the air release groove are intermittently communicated through the air guiding groove, enabling the gas inside the incubator to react stably with the microorganisms. And when the gas pressure inside the incubator gradually increases, the gas pressure overcomes the elastic force of the first elastic member, causing the blocking block to disengage from the air outlet groove, facilitating the discharge of the gas inside the incubator, providing a stable gas environment for the growth of microorganisms. And according to the different swinging frequencies of the incubator, the gas discharge interval inside the incubator will also increase, enabling the pure gas entering the incubator to stably contact and react with the microorganisms, and effectively improving the effect of microorganism cultivation. BRIEF DESCRIPTION OF THE DRAWINGS

[0018] Figure 1 is a schematic diagram of the whole of the present invention; Figure 2 is a sectional view of the present invention; Figure 3 is a schematic diagram of the structure at the incubator of the present invention; Figure 4 is a schematic diagram of the structure at the supporting block of the present invention; Figure 5 is a sectional view of the incubator and the supporting block of the present invention; Figure 6 of the present invention Figure 5 is an enlarged view of part A; Figure 7 is a sectional view of the structure at the auxiliary block of the present invention; Figure 8 is a schematic diagram of the displayed structure of the exhaust mechanism of the present invention; Figure 9 is a schematic diagram of the whole of the air supplement mechanism of the present invention; Figure 10 is a sectional view of the structure of the air supplement mechanism of the present invention; Figure 11This is a schematic structural diagram of the adjustment mechanism in the present invention.

[0019] In the figure: 1, cabinet body; 2, constant temperature box; 3, incubator; 4, air supplement mechanism; 401, connecting block; 402, air supplement channel; 403, connecting pipe; 404, air supplement pipe; 5, adjustment mechanism; 501, accommodation groove; 502, rotating ring; 503, adjustment plate; 504, arc groove; 505, baffle; 506, insertion rod; 507, guiding groove; 508, guiding rod; 509, ejector rod; 510, pressing block; 511, electric telescopic rod; 512, second elastic member; 6, exhaust mechanism; 601, exhaust groove; 602, blocking plate; 603, air outlet groove; 604, blocking block; 605, positioning ring; 606, first elastic member; 607, auxiliary block; 608, air release groove; 609, slideway; 610, intermittent plate; 611, air guiding groove; 612, piston chamber; 613, piston rod; 7, supporting block; 8, driving motor; 9, limiting ring; 10, disc; 11, push rod; 12, mass sensor; 13, cabinet door; 14, transparent plate; 15, card slot. Detailed implementation manners

[0020] Next, the technical solutions in the embodiments of the present invention will be clearly and completely described in conjunction with the accompanying drawings in the embodiments of the present invention. Obviously, the described embodiments are only a part of the embodiments of the present invention, rather than all the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by those of ordinary skill in the art without creative efforts shall fall within the protection scope of the present invention.

[0021] Embodiment 1 When culturing microorganisms for inspection, it is usually necessary to use a culture device to provide a suitable environment for the growth of microorganisms, and at the same time, a shaking method is also used to assist in the cultivation of microorganisms. However, during the cultivation process, since microorganisms have different growth stages, and the amount of gas required in different growth stages is also different. Currently, the equipment can only shake to assist in the cultivation of microorganisms and cannot adjust the amount of gas required according to the state of different growth cycles of microorganisms. Therefore, this embodiment is specifically invented based on the above problems.

[0022] As Figure 1 - Figure 11As shown in the figure, this embodiment provides a medical test microbial culture device, including a cabinet body 1, a constant temperature box 2 fixedly connected inside the cabinet body 1, a culture box 3 slidably connected inside the constant temperature box 2, an air supplement mechanism 4 connected between the culture box 3 and the constant temperature box 2, the air supplement mechanism 4 is used to supplement gas into the culture box 3, an adjustment mechanism 5, the adjustment mechanism 5 is arranged inside the air supplement mechanism 4 and is used to adjust the amount of gas supplemented into the culture box 3 by the air supplement mechanism 4, and an exhaust mechanism 6, the exhaust mechanism 6 is arranged on the side of the culture box 3 away from the air supplement mechanism 4 and is used to intermittently adjust the gas density inside the culture box 3; When the culture box 3 slides, the culture box 3 can adjust the air intake of the air supplement mechanism 4 through the adjustment mechanism 5, and enable the exhaust mechanism 6 to open and close intermittently, the gas pressure inside the culture box 3 increases, so that the gas inside the culture box 3 is discharged through the exhaust mechanism 6.

[0023] The air supplement mechanism 4 includes a connection block 401 fixedly connected to the constant temperature box 2, an air supplement channel 402 is opened inside the connection block 401, one end of the air supplement channel 402 close to the culture box 3 is communicated with the culture box 3 through a connecting pipe 403, and one end of the air supplement channel 402 away from the culture box 3 is fixedly connected with an air supplement pipe 404, and the free end of the air supplement pipe 404 extends out of the constant temperature box 2 and the cabinet body 1 in sequence.

[0024] The adjustment mechanism 5 includes a receiving groove 501 opened on the connection block 401, a rotating ring 502 is rotatably connected inside the receiving groove 501, one end of the rotating ring 502 close to the connecting pipe 403 is fixedly connected with an adjustment plate 503, a plurality of arc-shaped grooves 504 are circumferentially and spacedly arranged on the adjustment plate 503, and a plurality of baffle plates 505 corresponding to the arc-shaped grooves 504 are slidably connected inside the connection block 401, the plurality of baffle plates 505 can block the air supplement channel 402, one end of each baffle plate 505 away from the air supplement channel 402 is fixedly connected with an insertion rod 506, and the free end of each insertion rod 506 is inserted into the corresponding arc-shaped groove 504. When the adjustment plate 503 rotates, the plurality of insertion rods 506 can slide synchronously along the arc-shaped grooves 504 towards the air supplement channel 402.

[0025] An inclined guiding groove 507 is opened on the circumferential side of the rotating ring 502, a vertically extending guiding rod 508 is slidably connected to the connection block 401, a top rod 509 is fixedly connected to the side of the guiding rod 508 close to the rotating ring 502, the top rod 509 is slidably connected with the guiding groove 507, a pressing block 510 is arranged on the side of the culture box 3 close to the connection block 401, the side of the pressing block 510 close to the connection block 401 is a wedge-shaped surface, one end of the guiding rod 508 close to the culture box 3 extends out of the connection block 401 and can contact with the pressing block 510, an electric telescopic rod 511 is connected inside the culture box 3, the output end of the electric telescopic rod 511 is fixedly connected with the pressing block 510, and the guiding rod 508 is connected with the connection block 401 through a second elastic member 512.

[0026] A supporting block 7 is fixedly connected inside the incubator 2 and located below the culture box 3. A driving motor 8 is installed on the supporting block 7. A limiting ring 9 is fixedly connected to one side of the culture box 3 close to the driving motor 8. The output end of the driving motor 8 is coaxially fixedly connected with a disc 10. An ejector rod 11 is eccentrically fixedly connected to the disc 10. The ejector rod 11 extends into the limiting ring 9. A mass sensor 12 is connected to the culture box 3. The mass sensor 12 is electrically connected to the driving motor 8 and the electric telescopic rod 511. A cabinet door 13 capable of sealing the incubator 2 is hinged on the cabinet body 1. A transparent plate 14 capable of sealing the opening of the culture box 3 is hinged at the opening of the culture box 3. A card slot 15 is opened on the inner wall of the culture box 3.

[0027] During use, first open the cabinet door 13 inside the cabinet body 1 and open the transparent plate 14 that seals the culture box 3. Place the culture dish or culture bottle containing microorganisms in the card slot 15 inside the culture box 3 to prevent the culture dish or culture bottle from sliding when the culture box 3 shakes. After placing, make the transparent plate 14 seal the opening of the culture box 3 again to ensure the airtightness inside the culture box 3. Then close the cabinet door 13 to provide a stable and reliable growth environment. Then start the driving motor 8. The driving motor 8 drives the ejector rod 11 to rotate through the disc 10. When the eccentric point of the disc 10 and the ejector rod 11 rotates from the frontmost position to the rightmost position, at this time, the disc 10 drives the culture box 3 to slide along the supporting block 7 towards the direction close to the connecting block 401 through the ejector rod 11 and the limiting ring 9, so that the culture box 3 drives the culture medium inside it to shake through the culture dish or culture bottle, facilitating the full contact and reaction between the microorganisms and the culture medium.

[0028] When the culture box 3 drives the pressing block 510 towards the connecting block 401, and since the side of the pressing block 510 close to the connecting block 401 is an inclined surface, after the pressing block 510 contacts the guiding rod 508, the wedge-shaped surface on the pressing block 510 can drive the guiding rod 508 to slide along the connecting block 401, so that the guiding rod 508 drives the ejector rod 509 to move, and the second elastic member 512 is compressed. And the opened guiding groove 507 is arranged in an inclined state. The guiding rod 508 drives the ejector rod 509 to slide along the guiding groove 507, so that the ejector rod 509 drives the rotating ring 502 to rotate along the accommodating groove 501 through the guiding groove 507, so that the rotating ring 502 drives the adjusting plate 503 to rotate synchronously. Since the distance between the opened arc-shaped groove 504 and the center of the adjusting plate 503 gradually increases in the clockwise direction, the adjusting plate 503 drives a plurality of ejector rods 506 to slide radially outwards through a plurality of arc-shaped grooves 504. The plurality of ejector rods 506 drive a plurality of baffle plates 505 to slide radially outwards along the air supplement channel 402, so that the plurality of baffle plates 505 gradually release from the sealing state of the air supplement channel 402. And the air supplement pipe 404 is communicated with an external pure gas container, so that pure gas flows into the culture box 3 through the air supplement pipe 404, the air supplement channel 402 and the connecting pipe 403, so that pure gas can also provide the gas environment for the growth of microorganisms.

[0029] When the eccentric point of the disc 10 and the push rod 11 rotates from the rightmost side to the leftmost side, at this time, the disc 10 drives the incubator 3 to slide along the supporting block 7 away from the connecting block 401 through the push rod 11 and the limiting ring 9, so that the pressing block 510 no longer presses the guide rod 508. Under the action of the second elastic member 512, the second elastic member 512 drives the guide rod 508 to gradually slide to the initial position, so that the guide rod 508 drives the ejector rod 509 to slide along the guide groove 507, so that the ejector rod 509 drives the rotating ring 502 to rotate along the receiving groove 501 through the guide groove 507, so that the rotating ring 502 drives the adjusting plate 503 to rotate synchronously, so that the adjusting plate 503 drives a plurality of inserting rods 506 to slide radially inwards through a plurality of arc-shaped grooves 504, and the plurality of inserting rods 506 drive a plurality of baffle plates 505 to slide radially inwards along the air supplementing channel 402, so that the plurality of baffle plates 505 block the air supplementing channel 402 again to prevent excessive pure gas from entering the incubator 3.

[0030] Since the moving distance of the pressing block 510 is not more than the distance of the guide rod 508, when the mass sensor 12 detects that the mass on the incubator 3 changes rapidly, at this time, the microorganisms enter the rapid growth period, so that the mass sensor 12 transmits a signal to the driving motor 8 and the electric telescopic rod 511, so that the rotation speed of the driving motor 8 decreases and the swing frequency of the incubator 3 decreases. At the same time, the electric telescopic rod 511 expands and contracts to drive the pressing block 510 to move. Since the vertical distance between the pressing block 510 and the guide rod 508 decreases, the distance that the pressing block 510 pushes the guide rod 508 to slide along the connecting block 401 increases. Thus, the angle by which the guide rod 508 drives the rotating ring 502 to rotate through the ejector rod 509 and the guide groove 507 increases, so that the distance that the plurality of baffle plates 505 slide radially outwards increases, thereby reducing the blocking area of the air supplementing channel 402, increasing the air intake of the air supplementing channel 402, increasing the amount of pure gas replenished into the incubator 3, so that the pure gas can meet the gas conditions for the rapid growth of microorganisms and is convenient for the microorganisms to grow more comprehensively.

[0031] When the growth cycle of the microorganisms enters the stable period and the decline period, at this time, the mass change of the microorganisms is no longer rapid, so that the mass sensor 12 transmits the detection signal to the electric telescopic rod 511, so that the electric telescopic rod 511 drives the pressing block 510 to gradually slide towards the incubator 3, so that the stroke of the pressing block 510 pushing the guide rod 508 gradually decreases, thereby being able to adjust the area of the air supplementing channel 402 blocked by the plurality of baffle plates 505, so that the pure gas entering the incubator 3 conforms to the gas environment during the growth of the microorganisms, thereby further improving the effect of microorganism culture.

[0032] In summary, through the quality changes in different growth cycles of microorganisms, the quality sensor 12 adjusts the position of the pressing block 510 through the electric telescopic rod 511, so that the area of the air supply channel 402 blocked by the plurality of baffles 505 can be adjusted through the pressing block 510, the guiding rod 508 and the rotating ring 502. Thus, the air intake volume of the air supply channel 402 can be automatically adjusted during different growth cycles of microorganisms, the air intake volume can be reasonably controlled, which is convenient for meeting the gas requirements of microorganisms in different growth cycles and facilitating the better growth of microorganisms.

[0033] Embodiment 2 After a large amount of pure gas is injected into the incubator 3, and during the rapid growth of microorganisms, gases such as carbon dioxide can be generated, causing the gas pressure in the incubator 3 to gradually increase. Moreover, the mixture of the gases generated by the microorganisms themselves and the pure gas is also not conducive to the growth of microorganisms. When the incubator 3 is connected to the constant temperature box 2 through a hole, the gas in the incubator 3 can be directly discharged, but the contact reaction time between the pure gas and the microorganisms is reduced, which is not conducive to the better growth of microorganisms. Therefore, further improvements are made based on the above embodiments.

[0034] As Figure 5 - Figure 11 shown, the exhaust mechanism 6 includes an exhaust groove 601 opened on the incubator 3 and horizontally penetrating. A sealing plate 602 is slidably connected in the exhaust groove 601. A plurality of air outlet grooves 603 are circumferentially and spacedly arranged on the sealing plate 602. A plurality of blocking blocks 604 corresponding to the air outlet grooves 603 are fixedly connected in the exhaust groove 601. Each blocking block 604 can extend into the corresponding air outlet groove 603. A positioning ring 605 is fixedly connected in the exhaust groove 601. The positioning ring 605 and the sealing plate 602 are connected by a first elastic member 606. An auxiliary block 607 is fixedly connected to one side of the incubator 3 close to the exhaust groove 601. An air release groove 608 communicating with the air outlet groove 603 is opened on the auxiliary block 607. A slideway 609 communicating with the air release groove 608 is opened on the auxiliary block 607. An intermittent plate 610 blocking the air release groove 608 is slidably connected in the slideway 609. An air guiding groove 611 capable of being located in the air release groove 608 is opened on the intermittent plate 610. One end of the sealing plate 602 away from the incubator 3 can be inserted into the air guiding groove 611. A piston chamber 612 communicating with the slideway 609 is opened in the auxiliary block 607. A piston rod 613 is slidably connected in the piston chamber 612. One end of the piston rod 613 away from the incubator 3 can contact the inner wall of the constant temperature box 2. When the piston rod 613 slides, it can drive the intermittent plate 610 to slide.

[0035] During use, when the eccentric point of the disc 10 and the push rod 11 rotates from the most forward side to the leftmost position, the incubator 3 drives the auxiliary block 607 to move away from the connecting block 401, causing the side wall of the thermostat 2 to squeeze the piston rod 613, so that the piston rod 613 slides along the piston chamber 612 towards the incubator 3, causing the piston rod 613 to squeeze the gas in the piston chamber 612. Under the action of the gas, the intermittent plate 610 slides along the slideway 609, causing the intermittent plate 610 to drive the air guide groove 611 to slide into the air release groove 608. At this time, since the gas pressure in the incubator 3 is slightly higher, the gas in the incubator 3 drives the plugging plate 602 to slide along the exhaust groove 601, and the first elastic member 606 is compressed, causing the end of the plugging plate 602 away from the incubator 3 to insert into the air guide groove 611. Moreover, the shape of the air guide groove 611 is spline-shaped, enabling the air release groove 608 and the exhaust groove 601 to communicate through the air guide groove 611. At this time, the air outlet groove 603 on the plugging plate 602 has not yet separated from the plugging block 604. During the process of the gas pressure in the incubator 3 gradually increasing, the gas pressure overcomes the elastic force of the first elastic member 606, causing the air outlet groove 603 on the plugging plate 602 to separate from the plugging block 604, and the exhaust groove 601 communicates with the incubator 3 through the air outlet groove 603, enabling the gas in the incubator 3 to be discharged into the thermostat 2 through the exhaust groove 601 and the air release groove 608.

[0036] After the incubator 3 exhausts and returns to the normal pressure, at this time, under the action of the first elastic member 606, the first elastic member 606 drives the plugging plate 602 to slide to the initial state through the positioning ring 605, causing the plugging block 604 to extend into the air outlet groove 603 again, thereby blocking the exhaust groove 601. At the same time, the plugging plate 602 no longer inserts into the air guide groove 611. Under the action of the gas in the piston chamber 612, the piston rod 613 slides to the initial position along the piston chamber 612, and the intermittent plate 610 blocks the air release groove 608 again, enabling the gas in the incubator 3 to be discharged intermittently and according to the pressure magnitude, facilitating the provision of a stable gas environment for the growth of microorganisms, and thus enabling the comprehensive cultivation of microorganisms. It should be noted that one-way valves are provided in both the air release groove 608 and the connecting pipe 403, enabling the gas to flow unidirectionally from the connecting pipe 403 to the incubator 3 and the gas to flow unidirectionally from the incubator 3 to the air release groove 608.

[0037] In summary, the oscillation of the incubator 3 can intermittently squeeze the piston rod 613, so that the exhaust groove 601 and the air release groove 608 are intermittently connected through the air guide groove 611, enabling the gas in the incubator 3 to stably react with the microorganisms. And when the gas pressure in the incubator 3 gradually increases, the gas pressure overcomes the elastic force of the first elastic member 606, causing the plugging block 604 to disengage from the air outlet groove 603, facilitating the discharge of the gas in the incubator 3, providing a stable gas environment for the growth of microorganisms. And according to the different oscillation frequencies of the incubator 3, the gas discharge interval in the incubator 3 will also increase, enabling the pure gas entering the incubator 3 to stably contact and react with the microorganisms, and effectively improving the effect of microorganism cultivation.

[0038] Embodiment 3 This embodiment also provides a medical test microorganism cultivation method, including the following steps: S1: First, place the culture dish or culture bottle containing microorganisms in the incubator 3, and then adjust the constant temperature box 2 to provide a stable growth environment.

[0039] S2: Then start the drive motor 8, and the drive motor 8 drives the microorganisms in the incubator 3 to shake, improving the efficiency of microorganism growth.

[0040] S3: Next, through the oscillation of the incubator 3, the air supplement mechanism 4 supplements gas into the incubator 3, and the adjustment mechanism 5 can adjust the gas volume according to different growth stages of the microorganisms.

[0041] S4: Finally, through the oscillation of the incubator 3, the exhaust mechanism 6 can be intermittently opened, thus ensuring the stability of the gas inside the incubator 3, which is beneficial to the growth of microorganisms. After cultivation, take out the culture dish or culture bottle.

[0042] The above shows and describes the basic principles, main features and advantages of the present invention. Those skilled in the art should understand that the present invention is not limited by the above embodiments. The above embodiments and the descriptions in the specification only illustrate the principles of the present invention. Without departing from the spirit and scope of the present invention, the present invention will have various changes and improvements, and these changes and improvements all fall within the scope of the present invention claimed. The scope of protection claimed by the present invention is defined by the appended claims and their equivalents.

Claims

1. A medical test microorganism culture device, including a cabinet body (1), wherein a thermostatic box (2) is fixedly connected inside the cabinet body (1), and it is characterized in that, A culture incubator (3) is slidably connected inside the constant temperature incubator (2). An air supplement mechanism (4) is connected between the culture incubator (3) and the constant temperature incubator (2). The air supplement mechanism (4) is used to supplement gas into the culture incubator (3). A regulating mechanism (5) is provided inside the air supplement mechanism (4) and is used to regulate the amount of gas supplemented into the culture incubator (3) by the air supplement mechanism (4). An exhaust mechanism (6) is provided on the side of the culture incubator (3) away from the air supplement mechanism (4) and is used to intermittently regulate the gas density inside the culture incubator (3). When the culture incubator (3) slides, the culture incubator (3) can regulate the air intake of the air supplement mechanism (4) through the regulating mechanism (5), and the exhaust mechanism (6) can be intermittently opened and closed, so that the gas pressure inside the culture incubator (3) increases, and the gas inside the culture incubator (3) is discharged through the exhaust mechanism (6).

2. The medical test microbial culture device according to claim 1, characterized in that: The air supplement mechanism (4) includes a connection block (401) fixedly connected to the constant temperature incubator (2). An air supplement channel (402) is opened inside the connection block (401). One end of the air supplement channel (402) close to the culture incubator (3) is communicated with the culture incubator (3) through a connecting pipe (403). The other end of the air supplement channel (402) away from the culture incubator (3) is fixedly connected to an air supplement pipe (404). The free end of the air supplement pipe (404) extends out of the constant temperature incubator (2) and the cabinet body (1) in sequence.

3. The medical test microbial culture device according to claim 2, characterized in that: The regulating mechanism (5) includes a receiving groove (501) opened on the connection block (401). A rotating ring (502) is rotatably connected inside the receiving groove (501). One end of the rotating ring (502) close to the connecting pipe (403) is fixedly connected to a regulating plate (503). A plurality of arc-shaped grooves (504) are circumferentially and spaced apart on the regulating plate (503). A plurality of baffles (505) corresponding to the arc-shaped grooves (504) are slidably connected inside the connection block (401). The plurality of baffles (505) can block the air supplement channel (402). One end of each baffle (505) away from the air supplement channel (402) is fixedly connected to an insertion rod (506). The free end of each insertion rod (506) is inserted into the corresponding arc-shaped groove (504). When the regulating plate (503) rotates, the plurality of insertion rods (506) can slide synchronously along the arc-shaped grooves (504) towards the air supplement channel (402).

4. A medical test microorganism culture device according to claim 3, characterized in that: An inclined guiding groove (507) is formed on the circumferential side of the swivel ring (502). A vertically extending guiding rod (508) is slidably connected to the connecting block (401). A push rod (509) is fixedly connected to the side of the guiding rod (508) close to the swivel ring (502). The push rod (509) is slidably connected to the guiding groove (507). A pressing block (510) is arranged on the side of the incubator (3) close to the connecting block (401). The side of the pressing block (510) close to the connecting block (401) is a wedge-shaped surface. The end of the guiding rod (508) close to the incubator (3) extends out of the connecting block (401) and can contact the pressing block (510). An electric telescopic rod (511) is connected in the incubator (3). The output end of the electric telescopic rod (511) is fixedly connected to the pressing block (510). The guiding rod (508) and the connecting block (401) are connected by a second elastic member (512).

5. The medical test microorganism culture device according to claim 4, characterized in that: The exhaust mechanism (6) includes an exhaust groove (601) formed on the incubator (3) and horizontally penetrating. A sealing plate (602) is slidably connected in the exhaust groove (601). A plurality of air outlet grooves (603) are circumferentially and spacedly formed on the sealing plate (602). A plurality of blocking blocks (604) corresponding to the air outlet grooves (603) are fixedly connected in the exhaust groove (601). Each blocking block (604) can extend into the corresponding air outlet groove (603). A positioning ring (605) is fixedly connected in the exhaust groove (601). The positioning ring (605) and the sealing plate (602) are connected by a first elastic member (606).

6. The medical test microorganism culture device according to claim 5, wherein: An auxiliary block (607) is fixedly connected to the side of the incubator (3) close to the exhaust groove (601). An air release groove (608) communicating with the air outlet groove (603) is formed in the auxiliary block (607). A slideway (609) communicating with the air release groove (608) is formed in the auxiliary block (607). An intermittent plate (610) for blocking the air release groove (608) is slidably connected in the slideway (609). An air guiding groove (611) capable of being located in the air release groove (608) is formed in the intermittent plate (610). The end of the sealing plate (602) away from the incubator (3) can be inserted into the air guiding groove (611).

7. The medical test microbial culture device according to claim 6, wherein: A piston chamber (612) communicating with the slideway (609) is formed in the auxiliary block (607). A piston rod (613) is slidably connected in the piston chamber (612). The end of the piston rod (613) away from the incubator (3) can contact the inner wall of the thermostat (2). When the piston rod (613) slides, it can drive the intermittent plate (610) to slide.

8. A medical test microorganism culture device according to claim 7, characterized in that: A supporting block (7) is fixedly connected inside the incubator (2) and located below the culture box (3). A driving motor (8) is installed on the supporting block (7). A limiting ring (9) is fixedly connected to one side of the culture box (3) close to the driving motor (8). The output end of the driving motor (8) is coaxially and fixedly connected with a disc (10). A push rod (11) is eccentrically fixedly connected to the disc (10). The push rod (11) extends into the limiting ring (9). A mass sensor (12) is connected to the culture box (3). The mass sensor (12) is electrically connected to the driving motor (8) and the electric telescopic rod (511).

9. The medical test microorganism culture device according to claim 8, wherein: A cabinet door (13) capable of sealing the incubator (2) is hinged on the cabinet body (1). A transparent plate (14) capable of sealing the opening of the culture box (3) is hinged at the opening of the culture box (3). A clamping groove (15) is formed on the inner wall of the culture box (3).

10. A method for culturing microorganisms in medical tests, using a medical test microorganism culturing device according to any one of claims 1-9, characterized in that, Including the following steps: S1: First, place the culture dish or culture bottle containing microorganisms in the culture box (3), and then adjust the incubator (2) to provide a stable growth environment; S2: Then start the driving motor (8), and the driving motor (8) drives the microorganisms in the culture box (3) to shake, improving the efficiency of microorganism growth; S3: Next, through the shaking of the culture box (3), the air supplement mechanism (4) supplements gas into the culture box (3), and the adjustment mechanism (5) can adjust the gas volume according to different growth stages of microorganisms; S4: Finally, through the shaking of the culture box (3), the exhaust mechanism (6) can be intermittently opened, thereby ensuring the stability of the gas inside the culture box (3), which is beneficial to the growth of microorganisms. After the cultivation is completed, take out the culture dish or culture bottle.

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