Method and device for cultivating green alga adhesion layer on surface of silicone rubber

Abstract

The invention discloses a silicon rubber surface green algae adhesion layer cultivation method and a cultivation device, the method comprises algae concentration, surface pretreatment, spraying treatment, green algae inoculation and cultivation, the cultivation method specifically comprises the following steps: placing an inoculated silicon rubber sample in the cultivation device, controlling the temperature to be 25-30 DEG C, the humidity to be 70-90%, the illumination period to be 12 h / 12 h, the illumination intensity to be 100-200 mol.m <-2 >. S <-1 >, and the illumination time to be 12 h / 12 h; and continuously supplying the culture solution through the hanging type dropper to maintain the growth of the green algae, and continuously supplying the culture solution through the hanging type dropper to maintain the growth of the green algae. The method can simulate light, temperature and gas conditions in a natural environment, and realizes period-controllable and density-controllable green alga adhesion layer construction in a laboratory environment by combining a microbial culture technology, so that the rule of influence of green alga adhesion on the performance of the composite insulator is more accurately mastered; scientific basis and technical support are provided for prevention and treatment of external insulation biological contamination of an electric power system.

Description

Technical Field

[0001] The present application relates to the technical field of external insulation protection of power systems, and particularly to a method for cultivating a green algae attachment layer on the surface of silicone rubber and a cultivation device therefor. Background Art

[0002] With the development of power systems towards higher voltages and larger capacities, composite insulators based on silicone rubber have become indispensable key equipment in power transmission and transformation projects due to their excellent anti-fouling flashover performance and corrosion resistance. However, in southern regions with significant humid and hot climates, biofouling phenomena are prevalent on the surfaces of composite insulators during long-term operation. In particular, the attachment and growth of microorganisms such as green algae not only change the physical and chemical properties of the material surface but may also trigger flashover accidents along the surface of the insulator. Existing research shows that the formation of green algae biofilms is closely related to environmental temperature and humidity, light intensity, and material surface characteristics, but there are still gaps in the systematic research on its mechanism of action.

[0003] Current research on biofouling of composite insulators mainly focuses on the field of chemical fouling, and insufficient attention is paid to the particularity of the biofouling layer. There is no established quantitative evaluation system for problems such as the hindrance of surface hydrophobicity migration, deterioration of the microstructure, and attenuation of electrical performance caused by green algae attachment. Due to the lack of an artificial cultivation method under laboratory-controlled conditions, existing research mostly relies on on-site sampling and analysis, making it difficult to conduct comparative tests at different growth stages, resulting in significant limitations in the understanding of the formation law, attachment mechanism, and interaction with materials of the green algae fouling layer. Therefore, the present invention proposes a method for cultivating a green algae attachment layer on the surface of silicone rubber and a cultivation device therefor. Summary of the Invention

[0004] The embodiments of the present application provide a method for cultivating a green algae attachment layer on the surface of silicone rubber and a cultivation device therefor, enabling the simulation of light, temperature, water, and gas conditions in the natural environment, and combining microbial cultivation technology to achieve the construction of a green algae attachment layer with a controllable period and controllable density in a laboratory environment, thereby more accurately grasping the influence law of green algae attachment on the performance of composite insulators.

[0005] The first aspect of the present application provides a method for cultivating a green algae attachment layer on the surface of silicone rubber, including:

[0006] S1. Algae species concentration: Concentrating the green algae species solution to obtain a high-concentration green algae solution;

[0007] S2. Surface pretreatment: Cleaning and sterilizing the surface of the silicone rubber sample;

[0008] S3. Spraying treatment: Uniformly spraying the green algae culture solution on the surface of the silicone rubber sample to form a culture solution substrate;

[0009] S4. Green algae inoculation: Uniformly coating the high-concentration green algae solution on the surface of the culture solution substrate;

[0010] S5. Cultivation: Place the inoculated silicone rubber sample in a cultivation device, control the temperature at 25 - 30 °C, humidity at 70 - 90%, light cycle at 12 h / 12 h, and light intensity at 100 - 200 µmol·m -2 ·s -1 , and continuously supply the culture solution through a hanging dropper to maintain the growth of green algae.

[0011] Optionally, in step S1, a centrifuge is used to concentrate the green algae seed solution. The centrifugation speed is 3000 rpm, the centrifugation time is 5 minutes, and the concentration of the concentrated green algae solution is 10 7 cells / mL.

[0012] Optionally, in step S3, each liter of the green algae culture solution includes 2.0 - 3.0 g of sodium nitrate, 0.5 - 1.5 g of potassium dihydrogen phosphate, 0.5 - 1.0 g of magnesium sulfate, 0.5 - 1.0 g of potassium chloride, 0.1 - 0.3 g of sodium carbonate, 0.05 - 0.1 g of chelated iron, and 1.0 mL of trace element solution;

[0013] The trace element solution includes 0.1 - 0.3 g / L of zinc sulfate, 0.01 - 0.03 g / L of copper sulfate, 0.1 - 0.3 g / L of manganese sulfate, and 0.01 - 0.03 g / L of sodium molybdate.

[0014] Optionally, in step S4, the high-concentration green algae solution is evenly coated by a sterilized coating tool, and the coating amount is 0.1 mL / cm 2 .

[0015] Optionally, in step S5, the dropping speed of the hanging dropper is 2 drops per hour.

[0016] The second aspect of the present application provides a cultivation device for implementing the above-mentioned method for cultivating a green algae attachment layer on the surface of silicone rubber, which includes: an enclosed test chamber;

[0017] A glass dish for placing the silicone rubber sample, a humidity control system for adjusting the humidity in the enclosed test chamber, a temperature control system for adjusting the temperature in the enclosed test chamber, a light control system for providing adjustable light conditions, and a hanging dropper for continuously supplying the culture solution are arranged in the enclosed test chamber.

[0018] Optionally, the humidity control system includes a humidity sensor and a water mist generator;

[0019] The humidity sensor is electrically connected to the water mist generator.

[0020] Optionally, the temperature control system includes a temperature sensor, a heating controller, and a heating rod;

[0021] The temperature sensor is electrically connected to the heating controller;

[0022] The heating controller is electrically connected to the heating rod.

[0023] Optionally, the light control system includes a dimmable LED light source, a light controller, a light intensity sensor, and a timing module;

[0024] The dimmable LED light sources are evenly distributed on the inner top of the closed test chamber;

[0025] Both the light intensity sensor and the timing module are electrically connected to the light controller;

[0026] The light controller is electrically connected to the dimmable LED light source.

[0027] Optionally, a valve for adjusting the dropping speed is provided on the hanging dropper.

[0028] From the above technical solutions, it can be seen that the embodiments of the present application have the following advantages: By simulating the light, temperature, water vapor conditions in the natural environment and combining with the microorganism culture technology, the present invention realizes the construction of a controllable cycle and controllable density green algae attachment layer in the laboratory environment. This method breaks through the limitation of the environmental simulation accuracy in traditional laboratory research, provides a standardized experimental platform for revealing the influence law of the green algae biofilm on the performance of composite insulators, and has important significance for improving the external insulation protection technology level of the power system. Description of the Drawings

[0029] Figure 1 It is a flowchart of the method for cultivating the green algae attachment layer on the silicone rubber surface in the embodiment of the present application;

[0030] Figure 2 It is a structural schematic diagram of the device for cultivating the green algae attachment layer on the silicone rubber surface in the embodiment of the present application;

[0031] Among them, the reference numerals are:

[0032] 1 - closed test chamber, 2 - glass dish, 3 - water mist generator, 4 - hanging dropper, 5 - light control system, 6 - temperature sensor, 7 - heating controller, 8 - heating rod, 9 - humidity sensor. Detailed Embodiments

[0033] To enable those skilled in the art to better understand the solution of this application, the technical solution in the embodiments of this application will be clearly and completely described below in conjunction with the accompanying drawings in the embodiments of this application. Obviously, the described embodiments are only a part of the embodiments of this application, rather than all the embodiments. All other embodiments obtained by those of ordinary skill in the art based on the embodiments in this application without creative efforts shall fall within the scope of protection of this application.

[0034] In the description of this application, it should be noted that the orientation or positional relationship indicated by the terms "center", "upper", "lower", "left", "right", "vertical", "horizontal", "inner", "outer", etc. is based on the orientation or positional relationship shown in the accompanying drawings. It is only for the convenience of describing this application and simplifying the description, rather than indicating or implying that the device or element referred to must have a specific orientation, be constructed and operated in a specific orientation, and therefore should not be construed as a limitation of this application. In addition, the terms "first", "second", and "third" are only used for descriptive purposes and cannot be understood as indicating or implying relative importance.

[0035] Unless otherwise clearly specified and limited, the terms "installation", "connection", and "connection" shall be understood in a broad sense. For example, it can be a fixed connection, a detachable connection, or an integral connection; it can be a mechanical connection or an electrical connection; it can be directly connected, or indirectly connected through an intermediate medium, and it can be the communication inside two elements. For those of ordinary skill in the art, the specific meanings of the above terms in this application can be understood according to specific situations.

[0036] This application provides an embodiment of a method for cultivating a green algae attachment layer on the surface of silicone rubber. For details, please refer to Figure 1 。

[0037] The method for cultivating a green algae attachment layer on the surface of silicone rubber in this embodiment includes:

[0038] S1. Algae species concentration: Concentrate the green algae species solution to obtain a high-concentration green algae solution;

[0039] S2. Surface pretreatment: Clean and sterilize the surface of the silicone rubber sample;

[0040] S3. Spraying treatment: Uniformly spray the green algae culture solution on the surface of the silicone rubber sample to form a culture solution substrate;

[0041] S4. Green algae inoculation: Uniformly coat the high-concentration green algae solution on the surface of the culture solution substrate;

[0042] S5. Cultivation: Place the inoculated silicone rubber sample in a culture device, control the temperature at 25 - 30 °C, the humidity at 70 - 90%, the light cycle at 12h / 12h, and the light intensity at 100 - 200 µmol·m-2 ·s -1 and continuously supply the culture solution through the hanging dropper 4 to maintain the growth of green algae.

[0043] It should be noted that: by simulating the light, temperature, water and gas conditions in the natural environment and combining with the microbial culture technology, the present invention realizes the construction of a controllable cycle and controllable density green algae attachment layer in the laboratory environment. This method breaks through the limitation of the environmental simulation accuracy of traditional laboratory research, provides a standardized experimental platform for revealing the influence law of green algae biofilm on the performance of composite insulators, and has important significance for improving the external insulation protection technology level of the power system.

[0044] The above is the first embodiment of a method for cultivating a green algae attachment layer on the surface of silicone rubber provided by this application. The following is the second embodiment of a method for cultivating a green algae attachment layer on the surface of silicone rubber provided by this application. For details, please refer to Figure 1 .

[0045] The method for cultivating a green algae attachment layer on the surface of silicone rubber in this embodiment includes:

[0046] S1. Algae species concentration: Concentrate the green algae species solution to obtain a high-concentration green algae solution;

[0047] S2. Surface pretreatment: Clean and sterilize the surface of the silicone rubber sample;

[0048] S3. Spraying treatment: Uniformly spray the green algae culture solution on the surface of the silicone rubber sample to form a culture solution substrate;

[0049] S4. Green algae inoculation: Uniformly coat the high-concentration green algae solution on the surface of the culture solution substrate;

[0050] S5. Cultivation: Place the inoculated silicone rubber sample in a cultivation device, and maintain appropriate temperature, humidity and light conditions through the cultivation device to promote the growth of green algae; specifically, control the temperature at 25 - 30 °C, humidity at 70 - 90%, light cycle at 12h / 12h, and light intensity at 100 - 200 µmol·m -2 ·s -1 and continuously supply the culture solution through the hanging dropper 4 to maintain the growth of green algae (check and adjust the amount of the culture solution every day to ensure continuous supply of nutrients).

[0051] Specifically, the silicone rubber sample can be a composite insulator.

[0052] In step S1, a centrifuge is used to concentrate the green algae species solution, the centrifugation speed is 3000 rpm, the centrifugation time is 5 minutes, and the concentration of the concentrated green algae solution is 10 7 cells / mL.

[0053] In step S2, specifically, alcohol with a concentration of 75% can be used to wipe the surface of the silicone rubber sample to remove oil stains and impurities. After that, it is rinsed with distilled water and dried, and then sterilized by irradiating with an ultraviolet lamp.

[0054] In step S3, each liter of the green algae culture solution includes 2.0 - 3.0 g of sodium nitrate, 0.5 - 1.5 g of potassium dihydrogen phosphate, 0.5 - 1.0 g of magnesium sulfate, 0.5 - 1.0 g of potassium chloride, 0.1 - 0.3 g of sodium carbonate, 0.05 - 0.1 g of chelated iron (Fe-EDTA), and 1.0 mL of trace element solution; the trace element solution includes 0.1 - 0.3 g / L of zinc sulfate, 0.01 - 0.03 g / L of copper sulfate, 0.1 - 0.3 g / L of manganese sulfate, and 0.01 - 0.03 g / L of sodium molybdate.

[0055] In step S4, the high-concentration green algae solution is evenly coated by a sterilized coating tool (the sterilized coating tool can be a disinfected brush), and the coating amount is 0.1 mL / cm 2 . Specifically, an appropriate amount of the high-concentration green algae solution can be taken with a pipette and evenly dropped on the surface of the culture medium substrate according to the coating amount of 0.1 mL / cm 2 , and then the high-concentration green algae solution is gently brushed with a disinfected brush to ensure uniform coating.

[0056] In step S5, the dropping speed of the hanging dropper 4 is 2 drops per hour.

[0057] During specific implementation, 500 mL of the purchased green algae strain solution (with a concentration of 10 6 cells / mL) is taken and poured into a centrifuge tube. Using a high-speed centrifuge, it is centrifuged at a speed of 3000 rpm for 5 min to remove the supernatant. After centrifugation, the precipitated algae strain at the bottom is collected and resuspended with 50 mL of the culture solution to form a high-concentration green algae solution (with a concentration of 10 7 cells / mL).

[0058] Add 900 mL of distilled water to a beaker, and sequentially add 2.5 g of sodium nitrate, 1.0 g of potassium dihydrogen phosphate, 0.5 g of magnesium sulfate, 0.5 g of potassium chloride, and 0.2 g of sodium carbonate, and keep stirring well; then continue to add 0.05 g of chelated iron (note that it needs to be added slowly to avoid precipitation) to form the first solution. Add 900 mL of distilled water to another beaker, and sequentially add 0.1 g of zinc sulfate, 0.02 g of copper sulfate, 0.1 g of manganese sulfate, and 0.01 g of sodium molybdate, stir well, and make up to 1 L with distilled water to complete the preparation of the trace element solution. Take 1 mL of the trace element solution and add it to the first solution, and make up to 1 L with distilled water. Continue to use 1M hydrochloric acid or 1M sodium hydroxide solution to adjust the pH to the range of 7.0 - 7.5, stir well and then confirm the pH value again, and the preparation of the green algae culture solution is completed.

[0059] Filter the green algae culture solution through a 0.45 μm filter membrane to remove impurities and microorganisms. Aliquot the filtered green algae culture solution into culture bottles and sterilize it at a high temperature of 121 °C for 30 minutes, then cool it for standby. Dip a sterile gauze into 75% alcohol and wipe the silicone rubber sample taken to remove oil stains and impurities; then rinse the surface with distilled water and place it in a sterile environment to dry. Finally, irradiate the surface of the silicone rubber sample with an ultraviolet lamp for 30 minutes to complete the sterilization treatment. Take 100 mL of the high-temperature sterilized and cooled green algae culture solution, and use a spray gun to evenly spray it on the surface of the silicone rubber sample to form a thin liquid film (with a thickness of about 0.1 - 0.2 mm), and dry it at room temperature for about 10 minutes to solidify the thin liquid film into an attached film, forming a culture solution substrate.

[0060] Use a pipette to take 5 mL of high-concentration green algae solution and add it dropwise evenly at a rate of 0.1 mL / cm 2 onto the culture solution substrate, and further gently brush the high-concentration green algae solution with a sterilized brush to ensure uniform coverage. Fix the inoculated silicone rubber sample on the glass dish 2 in the culture device. Maintain the temperature at 25 °C through the temperature control system, maintain the humidity at 80% through the humidity control system, set the light cycle of the light control system 5 to 12 h / 12 h, adjust the valve of the hanging dropper 4, control the dropping rate of the green algae culture solution to 2 drops / h, check and adjust the amount of the culture solution every day to ensure continuous nutrient supply. Keep the above settings and continue to culture for 7 days, and finally a green algae attachment layer can be cultured on the surface of the silicone rubber sample.

[0061] As Figure 2 shown, the present application also provides a culture device for implementing the above-mentioned method for cultivating a green algae attachment layer on the surface of silicone rubber, which includes: a closed test chamber 1, a glass dish 2 for placing the silicone rubber sample (the silicone rubber sample can be a composite insulator) is arranged in the closed test chamber 1, a humidity control system for adjusting the humidity in the closed test chamber 1, a temperature control system for adjusting the temperature in the closed test chamber 1, a light control system 5 for providing adjustable light conditions, and a hanging dropper 4 for continuously supplying the culture solution.

[0062] It can be understood that this culture device can simulate the temperature, humidity, and light conditions in the natural environment and provide a suitable environment for the growth of green algae. Among them, the closed test chamber 1 is used to provide an independent and controllable culture environment. The glass dish 2 is placed horizontally at the center position of the inner bottom of the closed test chamber 1 as a placement platform for the silicone rubber sample.

[0063] The humidity control system includes a humidity sensor 9 and a water mist generator 3. The water mist generator 3 is symmetrically arranged on both sides of the closed test chamber 1 and is used to generate fine water mist to simulate the humidity conditions in the natural environment. The humidity sensor 9 is electrically connected to the water mist generator 3. Specifically, the water mist generator 3 is integrated with a humidity controller. The humidity sensor 9 is located at the lower left corner inside the closed test chamber 1 and is used to monitor the humidity inside the closed test chamber 1 in real time and transmit it to the humidity controller in the water mist generator 3. The humidity controller adjusts the self-power of the water mist generator 3 according to the humidity inside the closed test chamber 1 to achieve real-time adjustment of the humidity inside the closed test chamber 1.

[0064] The temperature control system includes a temperature sensor 6, a heating controller 7 and a heating rod 8. The temperature sensor 6 is electrically connected to the heating controller 7, and the heating controller 7 is electrically connected to the heating rod 8. Specifically, the temperature sensor 6 is located at the lower right corner inside the closed test chamber 1 and is used to monitor the temperature inside the closed test chamber 1 in real time and transmit it to the heating controller 7. The heating controller 7 controls the heating power of the heating rod 8 according to the internal temperature to achieve real-time adjustment of the temperature inside the closed test chamber 1.

[0065] The lighting control system 5 includes a dimmable LED light source, a lighting controller, a light intensity sensor and a timing module. The dimmable LED light sources are evenly distributed on the inner top of the closed test chamber 1. The light intensity sensor and the timing module are both electrically connected to the lighting controller, and the lighting controller is electrically connected to the dimmable LED light sources.

[0066] It should be noted that: the lighting control system 5 provides adjustable lighting conditions by artificially setting the lighting intensity and lighting change cycle to simulate the natural lighting cycle.

[0067] The hanging dropper 4 is suspended on the inner top of the closed test chamber 1 and is directly opposite to the silicone rubber sample. A valve for adjusting the dropping speed is provided on the hanging dropper 4, and the dropping speed is adjusted by artificially adjusting the opening degree of the valve.

[0068] Preferably, the inner wall of the closed test chamber 1 is coated with an anti-corrosion coating, and a sterile air circulation system is provided inside the closed test chamber 1.

[0069] As described above, the above embodiments are only used to illustrate the technical solutions of the present application, rather than to limit it; although the present application has been described in detail with reference to the foregoing embodiments, those of ordinary skill in the art should understand that: they can still modify the technical solutions recorded in the foregoing embodiments, or perform equivalent replacements on some of the technical features; and these modifications or replacements do not make the essence of the corresponding technical solutions deviate from the spirit and scope of the technical solutions of the embodiments of the present application.

Claims

1. A method for cultivating a green algae attachment layer on the surface of silicone rubber, characterized in that, It includes the following steps: S1. Algal species concentration: Concentrate the green algal species solution to obtain a high-concentration green algal solution; S2. Surface pretreatment: Clean and sterilize the surface of the silicone rubber sample; S3. Spraying treatment: Uniformly spray the green algal culture solution on the surface of the silicone rubber sample to form a culture solution substrate; S4. Green algal inoculation: Uniformly coat the high-concentration green algal solution on the surface of the culture solution substrate; S5. Cultivation: Place the inoculated silicone rubber samples in a cultivation device, control the temperature at 25 - 30 °C, humidity at 70 - 90%, light cycle at 12 h / 12 h, and light intensity at 100 - 200 µmol·m -2 ·s -1 , and continuously supply the culture solution through a hanging dropper to maintain the growth of green algae.

2. The method for cultivating a green algal attachment layer on the surface of silicone rubber according to claim 1, wherein In step S1, a centrifuge is used to concentrate the green algae seed solution. The centrifugation speed is 3000 rpm, the centrifugation time is 5 minutes, and the concentration of the concentrated green algae solution is 10 7 cells / mL.

3. The method for cultivating a green algal attachment layer on the surface of silicone rubber according to claim 1, wherein In step S3, each liter of the green algal culture solution includes 2.0 - 3.0 g of sodium nitrate, 0.5 - 1.5 g of potassium dihydrogen phosphate, 0.5 - 1.0 g of magnesium sulfate, 0.5 - 1.0 g of potassium chloride, 0.1 - 0.3 g of sodium carbonate, 0.05 - 0.1 g of chelated iron, and 1.0 mL of trace element solution; The trace element solution includes 0.1 - 0.3 g / L of zinc sulfate, 0.01 - 0.03 g / L of copper sulfate, 0.1 - 0.3 g / L of manganese sulfate, and 0.01 - 0.03 g / L of sodium molybdate.

4. The method for cultivating a green algal attachment layer on the surface of silicone rubber according to claim 1, wherein In step S4, the high-concentration green algae solution is evenly coated by a sterilized coating tool, and the coating amount is 0.1 mL / cm 2 .

5. The method for cultivating a green algal attachment layer on the surface of silicone rubber according to claim 1, wherein In step S5, the dropping rate of the hanging dropper is 2 drops per hour.

6. A culture device for implementing the method for cultivating a green algae attachment layer on the surface of silicone rubber according to any one of claims 1-5, characterized in that, It includes: A closed test chamber; Inside the closed test chamber, there are a glass dish for placing the silicone rubber sample, a humidity control system for adjusting the humidity inside the closed test chamber, a temperature control system for adjusting the temperature inside the closed test chamber, a light control system for providing adjustable light conditions, and a hanging dropper for continuously supplying the culture solution.

7. The silicon rubber surface green algae attachment layer cultivation device according to claim 6, characterized in that, The humidity control system includes a humidity sensor and a water mist generator; The humidity sensor is electrically connected to the water mist generator.

8. The silicon rubber surface green algae attachment layer cultivation device according to claim 6, characterized in that, The temperature control system includes a temperature sensor, a heating controller, and a heating rod; The temperature sensor is electrically connected to the heating controller; The heating controller is electrically connected to the heating rod.

9. The silicone rubber surface green algae attachment layer culture device according to claim 6, characterized in that, The light control system includes an adjustable light LED light source, a light controller, a light intensity sensor, and a timing module; The adjustable light LED light sources are uniformly distributed on the inner top of the closed test chamber; Both the light intensity sensor and the timing module are electrically connected to the light controller; The light controller is electrically connected to the adjustable light LED light source.

10. The silicone rubber surface green algae attachment layer culture device according to claim 6, characterized in that, A valve for adjusting the dropping rate is provided on the hanging dropper.

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