Application of SRR1 gene in regulation and control of basidiospore morphogenesis in post-meiosis stage
By knocking out the SRR1 gene to affect the development of basidiozoa in the post-meiosis stage of basidiozoa, the spore-free mutant was constructed using CRISPR/Cas9 technology, which solved the problem of basidiozoa formation in edible fungi, and achieved the promotion of spore-free strain cultivation and molecular breeding process.
CN120249081APending Publication Date: 2025-07-04SHANDONG AGRICULTURAL UNIVERSITY
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Patent Information
- Application Number
- CN202510345562.8
- Authority / Receiving Office
- CN · China
- Patent Type
- Applications(China)
- Current Assignee / Owner
- Filing Date
- 2025-03-24
- Publication Date
- 2025-07-04
AI Technical Summary
Technical Problem
The prior art is difficult to effectively control the formation of basophila spores in edible fungi, resulting in health threats, strain escape and ecological impact, and spore-free mutants have yield and quality defects in commercial applications.
Method used
By knocking out or regulating the SRR1 gene, it affects the development of basidiozoite in the postmeiosis stage, and uses CRISPR/Cas9 gene editing technology to construct spore-free mutants to achieve the cultivation of spore-free strains.
Benefits of technology
The successful creation of spore-free strains solved the health threats and ecological risks caused by basophils, provided the genetic resources of spore-free strains, and promoted the molecular breeding process of spore-free strains.
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Abstract
The invention discloses an application of an SRR1 gene in regulation and control of basidiospore morphogenesis in a post-meiosis stage, and belongs to the technical field of genetic engineering. Compared with a wild type, a constructed SRR1 gene deletion mutant Srr1 has the advantages that the pileus of a mature sporocarp of a Srr1 strain is white, and the Srr1 strain loses sporulation ability. Micromorphological analysis shows that a basidium of the Srr1 strain contains four cell nucleuses, basidium peduncle development is abnormal, the top end cannot expand to the final shape and size of basidium, and the result shows that the SRR1 gene causes a spore-free phenotype by influencing basidium peduncle development in the post-meiosis stage. Research results of the invention provide theoretical basis and gene resources for creation of spore-free strains, and are helpful for perfecting fungal development biology theoretical research related to sexual reproduction.
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