Allergen detection kit

Abstract

The invention relates to the technical field of kits, and particularly discloses an allergen detection kit which comprises a front cover plate, a partition plate, a detection box body, a deflection film, a cotton sliver and a rear cover plate which are sequentially arranged from top to bottom, a sample adding hole is formed in the front cover plate, the sample adding hole is communicated with a plurality of catching grooves, a plurality of openings are formed in the partition plate, a plurality of detection grooves are formed in the detection box body, and the deflection film is arranged in the detection box body. The detection groove, the opening and the buckling groove define a detection cavity used for containing a detection film strip, the front cover plate is communicated with an overflow flow channel on one side of the buckling groove, a first through hole and a second through hole are formed in the partition plate and the detection box body below the overflow flow channel respectively, and a third through hole and a fourth through hole are formed in one side, close to and away from the second through hole, of the detection groove respectively. The second through hole and the third through hole are in sealed communication at the bottom of the detection box body through a deflection film, the cotton sliver is arranged below the fourth through hole, and a gas hole is formed in the detection box body. The allergen detection device is simple in structure, high in detection efficiency and capable of achieving simultaneous detection of various allergens.

Description

Technical Field

[0001] The present invention belongs to the technical field of kits, and particularly relates to an allergen detection kit. Background Art

[0002] Allergic diseases are a group of diseases caused by the abnormal immune response of the body's immune system to harmless substances in the environment, and their incidence is continuously rising globally.

[0003] Epidemiological investigation results show that common allergic diseases include allergic rhinitis, asthma, conjunctivitis, eczema, food allergy, drug allergy, and anaphylactic shock, etc. Among them, food allergy is particularly common in children and adolescents. Common food allergens include eggs, peanuts, milk, soybeans, wheat, nuts, fish, and crustacean seafood, etc. These allergens can trigger the immune system to produce specific antibodies (such as IgE or IgG4), and then induce different types of allergic reactions. Therefore, the detection of specific antibodies is of great significance for identifying food intolerance types, guiding individualized diet management, and clinical diagnosis.

[0004] Currently, the commonly used allergen detection methods are mainly the traditional ELISA (enzyme-linked immunosorbent assay) method and the colloidal gold immunochromatography method. Although they have a certain clinical application basis, there are still significant deficiencies in actual detection. The detection process of the traditional ELISA method is relatively complex, requiring multiple incubations, washings, and enzymatic color reactions. The whole detection process takes a long time, has high requirements for the professional skills of operators and the experimental environment, and requires special equipment (such as an enzyme-labeled instrument) for result interpretation, making it difficult to quickly respond to the needs of point-of-care testing. At the same time, the traditional ELISA method is more suitable for centralized and batch testing sites, such as hospital laboratories or third-party medical laboratories. Although it can improve the overall detection efficiency and reduce the labor burden, the equipment investment and maintenance costs are high, resulting in high marginal costs for single or small-scale detections, and it is difficult to meet the actual needs of primary medical institutions, community health service points, or household users for "individualized detection", "portable detection", and "point-of-care testing". In contrast, the colloidal gold immunochromatography method has the advantages of simple operation, no need for complex instruments, and quick result interpretation, and has certain application prospects in preliminary screening tests. However, the sensitivity of this method is relatively low, and false negative or false positive results are likely to occur, affecting the accuracy of clinical judgment. In addition, when detecting high-concentration samples, due to uneven liquid distribution, a prozone phenomenon may occur, that is, due to excessive antigen, the formation of antigen-antibody complexes is inhibited, resulting in false negative results. At this time, the sample needs to be appropriately diluted and retested, increasing the complexity and time cost of the detection.

[0005] In summary, the existing allergen detection technologies still have obvious shortcomings in aspects such as detection process, sensitivity, specificity, portability, and cost control, effectively limiting their efficiency and reliability in clinical and practical applications.

[0006] Therefore, we propose an allergen detection kit to solve the above technical problems. Summary of the Invention

[0007] To solve the technical problems existing in the above-mentioned prior art, the present invention proposes an allergen detection kit.

[0008] The technical solution adopted by the present invention is as follows: An allergen detection kit includes a front cover plate, a partition plate, a detection box body, a deflection film, a cotton strip, and a rear cover plate arranged in sequence from top to bottom. The front cover plate is provided with a through sampling hole, and the sampling hole is communicated with a plurality of buckle grooves arranged side by side. The partition plate is provided with a plurality of openings. The top surface of the detection box body is provided with a plurality of detection grooves. The positions of the detection grooves, openings, and buckle grooves correspond to each other one by one, and enclose a detection chamber for accommodating a detection membrane strip. The front cover plate is communicated with an overflow channel on one side of each buckle groove. The partition plate and the detection box body are respectively provided with a first through hole and a second through hole with corresponding positions below the overflow channel. The detection groove is provided with a third through hole penetrating through on one side close to the second through hole and a fourth through hole penetrating through on one side far from the second through hole. The bottom of the detection box body is provided with a first diversion groove between the second through hole and the third through hole. The deflection film is attached to the bottom of the detection box body and covers and seals the second through hole, the first diversion groove, and the third through hole. The cotton strip is arranged below the fourth through hole. The detection box body is provided with a gas hole.

[0009] In a further technical solution, a shunt port is communicated with the bottom surface of the front cover plate below the sampling hole. The shunt port is communicated with a plurality of branch channels, and the branch channels are communicated with the buckle grooves.

[0010] In a further technical solution, the partition plate and the detection box body are respectively provided with a fifth through hole and a sixth through hole corresponding to the position of the sampling hole, and a seventh through hole and an eighth through hole corresponding to the position of the shunt port. The bottom of the detection box body is provided with a second diversion groove between the sixth through hole and the eighth through hole. The rear cover plate is attached to the bottom of the detection box body and covers and seals the sixth through hole, the second diversion groove, and the eighth through hole.

[0011] In a further technical solution, the gas hole includes an upper air hole, the upper air hole penetrates through the detection box body, and the rear cover plate is provided with a first exhaust hole corresponding to the position below the upper air hole.

[0012] In a further technical solution, the gas holes further include lower air holes, and the lower air holes include a first lower air hole and a second lower air hole. The first lower air hole and the second lower air hole are disposed through the detection box body. A partition is provided between the first lower air hole and the second lower air hole at the bottom of the detection box body. The first lower air hole is located on one side of the second through hole. A second exhaust hole is provided on the side wall of the detection box body on one side of the second lower air hole.

[0013] In a further technical solution, both the front cover plate and the partition are made of transparent plastic.

[0014] In a further technical solution, the preparation steps of the detection membrane strip are as follows: First, group according to food components, dissolve the purified food-specific protein in a buffer solution, and the working concentration is 1 mg / mL; Subsequently, use a film spraying instrument to evenly spray the buffer solutions of each group of dissolved food components on the nitrocellulose membrane at a certain interval; Finally, after drying, cut it into a membrane strip matching the size of the detection chamber, and put it into an aluminum foil bag, and place a desiccant for standby.

[0015] In a further technical solution, the pH value of the buffer solution is 7.2, and it includes 10 g / L of BSA (bovine serum albumin, hereinafter uniformly referred to as BSA), 0.1% of Proclin300 preservative in the total volume of the buffer solution, 0.05 g / L of TWEEN-20 (Tween-20, full name polyoxyethylene sorbitan monolaurate, used as a non-ionic surfactant, hereinafter uniformly referred to as TWEEN-20), 0.15 mol / L of NaCl solution and 0.05 mol / L of Tris-HCl solution.

[0016] In a further technical solution, the detection steps of the allergen detection kit include the following: Use a syringe to inject the sample to be tested from the sample adding hole. After waiting for the sample to be tested to incubate with the detection membrane strip for 30 minutes, wash it 3 times with the cleaning solution; Use a syringe to add the mouse anti-human IgG4 antibody reaction solution. After incubating for 20 minutes, wash it with the cleaning solution; Use a syringe to add the horseradish peroxidase-labeled goat anti-mouse antibody reaction solution. After incubating for 15 minutes, wash it with the cleaning solution, and then use a syringe to add the substrate solution, and observe the strip within 5 minutes; Use a syringe to add the termination solution to terminate the reaction and then interpret the result: the color-developing strip shows the corresponding result according to the antibody concentration; Among them, the reagents used in the detection include: Mouse anti-human IgG4 antibody reaction solution, using 0.1 mg / mL mouse anti-human IgG4 antibody, dissolved in a phosphate buffer solution with a pH of 7.4, containing 5 mg / mL BSA, 0.1% (by volume) Proclin 300 preservative, 0.15 mol / L NaCl solution and 0.01 mol / L phosphate buffer; Goat anti-mouse antibody reaction solution labeled with horseradish peroxidase, using 0.1 mg / mL goat anti-mouse antibody reaction solution labeled with horseradish peroxidase, dissolved in a solution containing 5 mg / mL BSA, 0.1% (by volume) Proclin 300 preservative, 0.15 mol / L NaCl solution and 0.01 mol / L phosphate buffer; Substrate solution, TMB single-component chromogenic solution, purchased from Suzhou Yak Chemical Reagent Co., Ltd., product number D0022; Stop solution, 0.5 mol / L H2SO4 solution; Washing solution, pH 7.2, including 10 g / L BSA, 0.1% (by volume) Proclin 300 preservative, 0.05 g / L TWEEN-20, 0.15 mol / L NaCl solution and 0.01 mol / L phosphate buffer.

[0017] In summary, due to the adoption of the above technical solutions, the beneficial effects of the present invention are: 1. The present invention does not rely on special instruments. It not only has a simple structure, reducing equipment investment and maintenance costs, but also is easy to carry and perform rapid on-site detection. It has low requirements for the professional skills of operators, effectively simplifies the detection process and operation steps, reduces the detection complexity and time cost, improves the accuracy and reliability of the detection results, and can effectively meet the actual needs of grass-roots medical institutions, community health service points or household users for "individualized detection", "portable detection" and "point-of-care testing".

[0018] 2. Relying on the design of the internal through holes and flow channels of the detection kit, the present invention guides the liquid to flow along a predetermined path, realizing an automated liquid dispensing and detection reaction process. The reagents flow through the upper and lower sides of the detection membrane strip in turn, achieving two-way infiltration from top to bottom, ensuring sufficient contact and reaction between the liquid reagent and the allergens on the detection membrane strip, effectively avoiding the "prozone phenomenon" caused by excessive sample concentration, uneven liquid distribution, etc. in traditional detection strips, reducing the occurrence of false negative or false positive results, improving the detection accuracy and stability, and reducing the time and resource waste caused by repeated detection.

[0019] 3. In each detection chamber of the present invention, the detection membrane strips can be respectively sprayed with different types of purified food-specific proteins, enabling the synchronous detection of multiple food-related allergens, improving the detection efficiency while effectively reducing the detection cost. BRIEF DESCRIPTION OF THE DRAWINGS

[0020] The present invention will be described by way of examples and with reference to the accompanying drawings, where: Figure 1 is a schematic structural diagram of the present invention; Figure 2 is a schematic diagram of the bottom structure of the front cover plate of the present invention; Figure 3 is a schematic structural diagram of the partition of the present invention; Figure 4 is a schematic diagram of the top surface structure of the detection box body of the present invention; Figure 5 is a schematic diagram of the bottom surface structure of the detection box body of the present invention.

[0021] Reference numerals: 1 - front cover plate, 2 - partition, 3 - detection box body, 4 - deflection membrane, 5 - cotton strip, 6 - rear cover plate, 7 - sample addition hole, 8 - buckle groove, 9 - opening, 10 - detection groove, 11 - overflow channel, 12 - first through hole, 13 - second through hole, 14 - third through hole, 15 - fourth through hole, 16 - first diversion groove, 17 - shunt port, 18 - bifurcated channel, 19 - fifth through hole, 20 - sixth through hole, 21 - seventh through hole, 22 - eighth through hole, 23 - upper air hole, 24 - first exhaust hole, 25 - first lower air hole, 26 - second lower air hole, 27 - partition, 28 - second exhaust hole, 29 - second diversion groove. DETAILED DESCRIPTION OF THE EMBODIMENTS

[0022] The following will clearly and completely describe the technical solutions in the embodiments of the present invention with reference to the accompanying drawings in the embodiments of the present invention. Obviously, the described embodiments are only a part of the embodiments of the present invention, rather than all of the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by those of ordinary skill in the art without creative efforts shall fall within the scope of protection of the present invention.

[0023] Embodiment: Refer to Figures 1 - 5, the present invention provides an allergen detection kit, which includes a front cover plate 1, a partition plate 2, a detection box body 3, a deflection film 4, a cotton strip 5, and a rear cover plate 6 arranged in sequence from top to bottom. The front cover plate 1 is provided with a through sampling hole 7, and four juxtaposed buckles 8 are communicated with the sampling hole 7. Four openings 9 are formed on the partition plate 2. The top surface of the detection box body 3 is provided with four detection grooves 10. The positions of the detection grooves 10, the openings 9, and the buckles 8 correspond one by one, and enclose four independent detection chambers for accommodating detection membrane strips. The front cover plate 1 is communicated with an overflow channel 11 on one side of each buckle 8. The partition plate 2 and the detection box body 3 are respectively provided with a first through hole 12 and a second through hole 13 corresponding in position below the overflow channel 11. The detection groove 10 is provided with a third through hole 14 penetrating through on one side close to the second through hole 13 and a fourth through hole 15 penetrating through on one side far from the second through hole 13. The bottom of the detection box body 3 is provided with a first diversion groove 16 between the second through hole 13 and the third through hole 14. The deflection film 4 is attached to the bottom of the detection box body 3 and covers and seals the second through hole 13, the first diversion groove 16, and the third through hole 14. The cotton strip 5 is arranged below the fourth through hole 15, and the detection box body 3 is provided with a gas hole.

[0024] In this embodiment, referring to Figure 2 , a diversion port 17 is communicated with the bottom surface of the front cover plate 1 below the sampling hole 7, and four bifurcated flow channels 18 are communicated with the diversion port 17. The bifurcated flow channels 18 are respectively communicated with the adjacent buckles 8.

[0025] In this embodiment, referring to Figure 1 、 Figure 3 、 Figure 4 and Figure 5 , the partition plate 2 and the detection box body 3 are respectively provided with a fifth through hole 19 and a sixth through hole 20 corresponding in position to the sampling hole 7, and a seventh through hole 21 and an eighth through hole 22 corresponding in position to the diversion port 17. The bottom of the detection box body 3 is provided with a second diversion groove 29 between the sixth through hole 20 and the eighth through hole 22. The rear cover plate 6 is attached to the bottom of the detection box body 3 and covers and seals the sixth through hole 20, the second diversion groove 29, and the eighth through hole 22.

[0026] In this embodiment, referring to Figure 1 、 Figure 4 and Figure 5 , the gas hole includes an upper air hole 23, and the upper air hole 23 penetrates through the detection box body 3. The rear cover plate 6 is provided with a first exhaust hole 24 corresponding in position below the upper air hole 23.

[0027] In this embodiment, referring to Figure 4 and Figure 5, the gas pores further include lower air holes, and the lower air holes include a first lower air hole 25 and a second lower air hole 26. The first lower air hole 25 and the second lower air hole 26 are arranged through the detection box body 3. A partition 27 is provided between the first lower air hole 25 and the second lower air hole 26 at the bottom of the detection box body 3. The first lower air hole 25 is located on one side of the second through hole 13. A second exhaust hole 28 is provided on the side wall of the detection box body 3 on one side of the second lower air hole 26.

[0028] In this embodiment, both the front cover plate 1 and the partition plate 2 are made of transparent plastic, which is convenient for observing the reaction process and results.

[0029] In this embodiment, the preparation steps of the detection membrane strip are as follows: First, group according to food components, dissolve the purified food-specific protein in a buffer solution, and the working concentration is 1 mg / mL; Subsequently, use a film spraying instrument to evenly spray the buffer solutions of each group of dissolved food components on the nitrocellulose membrane at a certain interval; Finally, after drying, cut it into a membrane strip matching the size of the detection chamber, and put it into an aluminum foil bag, and place a desiccant for standby.

[0030] In this embodiment, the pH value of the buffer solution is 7.2, and it includes 10 g / L of BSA, 0.1% of Proclin300 preservative in the total volume of the buffer solution, 0.05 g / L of TWEEN-20, 0.15 mol / L of NaCl solution, and 0.05 mol / L of hydrochloric acid solution of hydroxymethylaminomethane.

[0031] In this embodiment, the detection steps of the allergen detection kit include the following: Use a syringe to inject the sample to be tested from the sample adding hole 7. After waiting for the sample to be tested to incubate with the detection membrane strip for 30 minutes, wash it 3 times with the cleaning solution; Use a syringe to add the mouse anti-human IgG4 antibody reaction solution. After incubating for 20 minutes, wash it with the cleaning solution; Use a syringe to add the horseradish peroxidase-labeled goat anti-mouse antibody reaction solution. After incubating for 15 minutes, wash it with the cleaning solution, and then use a syringe to add the substrate solution (TMB chromogenic solution), and observe the strip within 5 minutes; Use a syringe to add the termination solution, terminate the reaction and then judge the result: the color development strip shows the corresponding result according to the antibody concentration; Among them, the reagents used in the detection include: Mouse anti-human IgG4 antibody reaction solution, using 0.1 mg / mL mouse anti-human IgG4 antibody, dissolved in a solution with a pH of 7.4, containing 5 mg / mL BSA, 0.1% (by volume of the buffer) Proclin 300 preservative, 0.15 mol / L NaCl solution, and 0.01 mol / L phosphate buffer; Goat anti-mouse antibody conjugated with horseradish peroxidase reaction solution, using 0.1 mg / mL goat anti-mouse antibody conjugated with horseradish peroxidase, dissolved in a solution containing 5 mg / mL BSA, 0.1% (by volume of the buffer) Proclin 300 preservative, 0.15 mol / L NaCl solution, and 0.01 mol / L phosphate buffer; Substrate solution, TMB single-component chromogenic solution, purchased from Suzhou Yak Chemical Reagent Co., Ltd., with the product number D0022; Stop solution, 0.5 mol / L H2SO4 solution; Washing solution, with a pH of 7.2, including 10 g / L BSA, 0.1% (by volume of the buffer) Proclin 300 preservative, 0.05 g / L TWEEN-20, 0.15 mol / L NaCl solution, and 0.01 mol / L phosphate buffer.

[0032] The allergen detection kit of the embodiment of the present invention forms a compact and easy-to-operate detection product through the combined structure of the front cover plate 1, partition plate 2, detection box body 3, deflection film 4, cotton strip 5, and rear cover plate 6, and is suitable for the detection of various allergens. Among them, a sample addition hole 7 is provided on the front cover plate 1 for adding reagents such as the sample to be tested, reaction solution, chromogenic solution, and washing solution. After the liquid enters through the sample addition hole 7, relying on the design of the internal through holes and flow channels of the detection kit, the liquid is guided to flow along a predetermined path, realizing an automated liquid distribution and detection reaction process, effectively reducing the operation difficulty, and is especially suitable for operation by grass-roots medical units or non-professional personnel.

[0033] Specifically, the flow path of the liquid in the detection kit is as follows: First, place the detection kit horizontally. Add liquid to the sample addition hole 7 through a syringe. The liquid flows downward through the fifth through-hole 19 and the sixth through-hole 20 in sequence, and enters the closed diversion flow channel jointly formed by the rear cover plate 6 and the bottom of the detection box body 3. In this flow channel, the liquid flows from the sixth through-hole 20 along the second diversion groove 29 to the eighth through-hole 22. Subsequently, the liquid flows upward through the seventh through-hole 21 and the diversion port 17. The liquid is diverted to each branch flow channel 18 through the diversion port 17 and enters each detection chamber respectively. After the liquid reaches the detection chamber, due to the spacing effect of the partition plate 2, the liquid is located above the detection membrane strip. While infiltrating the upper part of the detection membrane strip, it diffuses to the overflow flow channel 11 along the gap between the detection membrane strip and the buckle groove 8. Then, the excess liquid flows downward from the overflow flow channel 11, flows through the first through-hole 12 and the second through-hole 13 in sequence, and enters the closed diversion flow channel jointly formed by the deflection membrane 4 and the bottom of the detection box body 3. In this flow channel, the liquid flows from the second through-hole 13 along the first diversion groove 16 to the third through-hole 14. After that, the liquid flows upward along the third channel and enters the detection chamber again. At this time, the liquid is located below the detection membrane strip. While infiltrating the lower part of the detection membrane strip, it diffuses to the fourth through-hole 15 along the gap between the detection groove 10 and the detection membrane strip. Finally, the residual liquid is absorbed by the cotton strip 5 below the fourth through-hole 15 to prevent the residual liquid from interfering with the detection result. During this process, the liquid infiltrates from above and below the detection membrane strip, ensuring sufficient contact and reaction between the liquid reagent and the allergens on the detection membrane strip, effectively avoiding the "prozone phenomenon" caused by too high sample concentration and uneven liquid distribution in the traditional test strip, reducing the occurrence of false negative or false positive results, improving the detection accuracy and stability, and reducing the time and resource waste caused by repeated detection.

[0034] It is worth mentioning that the number of detection chambers of this detection kit can be designed according to the detection requirements. In this embodiment, the detection membrane strips in each detection chamber can be respectively sprayed with different kinds of purified food-specific proteins, such as dairy products, seafood, nuts, grains, etc., so as to realize the synchronous detection of multiple food-related allergens, improve the detection efficiency and effectively reduce the detection cost. In addition, the detection kit is reasonably provided with gas holes, a first exhaust hole 24 and a second exhaust hole 28, which can automatically adjust the internal and external air pressures during the liquid flow process to ensure that the liquid flows smoothly along the established path, avoiding abnormal fluid paths or bubble interference caused by air resistance, and further ensuring the accuracy of the detection result. In the actual detection steps, after the addition and reaction of each reagent are completed, it is washed with a cleaning solution. The purpose is to remove the excess reaction substances in the previous step to prevent them from interfering with the subsequent reaction steps, so as to ensure the specificity of the whole detection process and the reliability of the result.

[0035] Compared with the allergen detection methods in the prior art, this detection kit does not need to rely on special instruments. It not only has a simple structure, reducing the equipment investment and maintenance costs, but also is easy to carry and quickly detect on-site. It has low requirements for the professional skills of operators, effectively simplifies the detection process and operation steps, reduces the detection complexity and time cost, improves the accuracy and reliability of the detection results, and can effectively meet the actual needs of primary medical institutions, community health service points or household users for "individualized detection", "portable detection" and "point-of-care testing". In addition, this detection kit is not only applicable to the detection of IgG4 antibodies, but also applicable to the detection of IgE antibodies. Only the mouse anti-human IgG4 antibody reaction solution in the detection system needs to be replaced with the mouse anti-human IgE antibody reaction solution, and the rest of the operation processes are the same. The preparation method of the mouse anti-human IgE antibody reaction solution is as follows: use 0.1 mg / mL of mouse anti-human IgE antibody, dissolve it in a phosphate buffer solution with a pH value of 7.4, containing 5 mg / mL of BSA, 0.1% Proclin 300 preservative accounting for the total volume of the buffer solution, 0.15 mol / L of NaCl solution and 0.01 mo1 / L of phosphate buffer solution. Therefore, it can be widely applied to pre-clinical screening related to allergens, individualized health assessment and household self-test scenarios, and has good applicability, scalability and industrialization prospects.

[0036] The above are only the embodiments of the present invention, and do not limit the patent scope of the present invention accordingly. Any equivalent structure or equivalent process transformation made by using the content of the specification and drawings of the present invention, or directly or indirectly applied in other related technical fields, shall be similarly included in the patent protection scope of the present invention.

Claims

1. An allergen detection kit, characterized in that, It includes a front cover plate (1), a partition plate (2), a detection box body (3), a deflection film (4), a cotton strip (5) and a rear cover plate (6) which are arranged in sequence from top to bottom. A through sample adding hole (7) is provided on the front cover plate (1). A plurality of juxtaposed buckling grooves (8) are communicated with the sample adding hole (7). A plurality of openings (9) are formed on the partition plate (2). A plurality of detection grooves (10) are provided on the top surface of the detection box body (3). The positions of the detection grooves (10), the openings (9) and the buckling grooves (8) correspond to each other one by one, and enclose a detection chamber for accommodating a detection membrane strip. An overflow channel (11) is communicated and arranged on one side of each buckling groove (8) of the front cover plate (1). A first through hole (12) and a second through hole (13) with corresponding positions are respectively formed on the partition plate (2) and the detection box body (3) below the overflow channel (11). A third through hole (14) is penetrated and arranged on one side of the detection groove (10) close to the second through hole (13), and a fourth through hole (15) is penetrated and arranged on the side far from the second through hole (13). A first diversion groove (16) is arranged between the second through hole (13) and the third through hole (14) at the bottom of the detection box body (3). The deflection film (4) is attached to the bottom of the detection box body (3) and covers and seals the second through hole (13), the first diversion groove (16) and the third through hole (14). The cotton strip (5) is arranged below the fourth through hole (15). A gas hole is provided on the detection box body (3); A diversion port (17) is communicated and arranged on the bottom surface of the front cover plate (1) below the sample adding hole (7). A plurality of bifurcated flow channels (18) are communicated with the diversion port (17). The bifurcated flow channels (18) are communicated with the buckling grooves (8); A fifth through hole (19) and a sixth through hole (20) corresponding to the position of the sample adding hole (7), and a seventh through hole (21) and an eighth through hole (22) corresponding to the position of the diversion port (17) are respectively penetrated and arranged on the partition plate (2) and the detection box body (3). A second diversion groove (29) is arranged between the sixth through hole (20) and the eighth through hole (22) at the bottom of the detection box body (3). The rear cover plate (6) is attached to the bottom of the detection box body (3) and covers and seals the sixth through hole (20), the second diversion groove (29) and the eighth through hole (22); Among them, the usage method of the allergen detection kit is as follows: First, place the detection kit horizontally. Add liquid to the sample addition hole (7) through a syringe. The liquid flows downward through the fifth through-hole (19) and the sixth through-hole (20) in sequence, and enters the closed diversion channel jointly formed by the rear cover plate (6) and the bottom of the detection box body (3). In this channel, the liquid flows from the sixth through-hole (20) along the second diversion groove (29) to the eighth through-hole (22). Subsequently, the liquid flows upward through the seventh through-hole (21) and the diversion port (17), and the liquid is diverted to each branch channel (18) through the diversion port (17) and enters each detection chamber respectively. After the liquid reaches the detection chamber, due to the spacing effect of the partition plate (2), the liquid is located above the detection membrane strip. While infiltrating the upper part of the detection membrane strip, it diffuses to the overflow channel (11) along the gap between the detection membrane strip and the buckle groove (8). Then, the excess liquid flows downward from the overflow channel (11), flows through the first through-hole (12) and the second through-hole (13) in sequence, and enters the closed diversion channel jointly formed by the deflection membrane (4) and the bottom of the detection box body (3). In this channel, the liquid flows from the second through-hole (13) along the first diversion groove (16) to the third through-hole (14). After that, the liquid flows upward along the third channel and enters the detection chamber again. At this time, the liquid is located below the detection membrane strip. While infiltrating the lower part of the detection membrane strip, it diffuses to the fourth through-hole (15) along the gap between the detection groove (10) and the detection membrane strip. Finally, the residual liquid is absorbed by the cotton strip (5) below the fourth through-hole (15) to prevent the residual liquid from interfering with the detection result.

2. The allergen detection kit according to claim 1, wherein The gas hole includes an upper air hole (23), and the upper air hole (23) is penetrated and arranged on the detection box body (3). The rear cover plate (6) is provided with a first exhaust hole (24) corresponding to the position below the upper air hole (23).

3. The allergen detection kit according to claim 2, wherein The gas hole further includes a lower air hole, and the lower air hole includes a first lower air hole (25) and a second lower air hole (26). The first lower air hole (25) and the second lower air hole (26) are penetrated and arranged on the detection box body (3). A partition (27) is provided between the first lower air hole (25) and the second lower air hole (26) at the bottom of the detection box body (3). The first lower air hole (25) is located on one side of the second through-hole (13). A second exhaust hole (28) is provided on the side wall of the detection box body (3) on one side of the second lower air hole (26).

4. The allergen detection kit according to claim 1, wherein Both the front cover plate (1) and the partition plate (2) are made of transparent plastic.

5. An allergen detection kit according to claim 1, characterized in that, The preparation steps of the detection membrane strip are as follows: First, group according to food components, dissolve the purified food-specific protein in a buffer solution, and the working concentration is 1 mg / mL. Subsequently, use a film spraying instrument to evenly spray the buffer solutions of each group of dissolved food components on the nitrocellulose membrane at a certain interval. Finally, after drying, cut it into a membrane strip matching the size of the detection chamber, and put it into an aluminum foil bag, and place a desiccant for standby.

6. The allergen detection kit according to claim 5, wherein The pH value of the buffer solution is 7.2, which includes 10 g / L of BSA, 0.1% Proclin 300 preservative accounting for the total volume of the buffer solution, 0.05 g / L of TWEEN-20, 0.15 mol / L of NaCl solution and 0.05 mol / L of hydrochloric acid solution of hydroxymethylaminomethane.

7. An allergen detection kit according to claim 1, wherein, The detection steps of the allergen detection kit are as follows: Use a syringe to inject the sample to be tested from the sample loading hole (7). After waiting for the sample to be tested to incubate with the test strip for 30 minutes, wash it 3 times with the cleaning solution; Use a syringe to add the mouse anti-human IgG4 antibody reaction solution. After incubating for 20 minutes, wash it with the cleaning solution; Use a syringe to add the horseradish peroxidase-labeled goat anti-mouse antibody reaction solution. After incubating for 15 minutes, wash it with the cleaning solution, and then use a syringe to add the substrate solution and observe the strip within 5 minutes; Use a syringe to add the termination solution. After terminating the reaction, interpret the result: The color development strip shows the corresponding result according to the antibody concentration; Among them, the reagents used in the detection include: Mouse anti-human IgG4 antibody reaction solution, using 0.1 mg / mL of mouse anti-human IgG4 antibody, dissolved in a buffer solution with a pH value of 7.4, containing 5 mg / mL of BSA, 0.1% Proclin 300 preservative accounting for the total volume of the buffer solution, 0.15 mol / L of NaCl solution and 0.01 mo1 / L of phosphate buffer solution; Horseradish peroxidase-labeled goat anti-mouse antibody reaction solution, using 0.1 mg / mL of horseradish peroxidase-labeled goat anti-mouse antibody reaction solution, dissolved in a buffer solution containing 5 mg / mL of BSA, 0.1% Proclin 300 preservative accounting for the total volume of the buffer solution, 0.15 mol / L of NaCl solution and 0.01 mo1 / L of phosphate buffer solution; Substrate solution, TMB single-component color development solution; Termination solution, 0.5 mol / L of H2SO4 solution; Cleaning solution, with a pH value of 7.2, including 10 g / L of BSA, 0.1% Proclin 300 preservative accounting for the total volume of the buffer solution, 0.05 g / L of TWEEN-20, 0.15 mo1 / L of NaCl solution and 0.01 mo1 / L of phosphate buffer solution.

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