Tissue culture device for cell engineering experiment

Abstract

The invention relates to the technical field of seed and seedling cultivation, in particular to a cell engineering experiment tissue culture device which comprises a culture box, a treatment box is fixedly connected to one side of the culture box, a top frame is arranged on the inner wall of the treatment box in a sliding fit mode, and a potato placing frame is fixedly connected to the lower side of the top frame. A movable seat is arranged on one side of the potato placing frame in a sliding fit mode, a potato stem and block separating mechanism is arranged on the movable seat in a rotating connection mode, a transmission mechanism is arranged on the inner wall of one side of the processing box in a rotating connection mode, and a material receiving groove is formed in the inner wall of one side of the processing box in a sliding fit mode. According to the potato cutting device, the positioning plate drives the potatoes to rotate after fixing the potatoes, tubers at different positions on the potatoes can be automatically cut off by utilizing the rotatable semicircular cutter, the problems of non-uniform cutting, too deep cutting or too shallow cutting and the like in manual operation are avoided, the cutting efficiency, precision and consistency are remarkably improved, and interference of human factors is reduced.

Description

Technical Field

[0001] The present invention relates to the technical field of seed and seedling cultivation, and particularly to a tissue culture device for cell engineering experiments. Background Art

[0002] In cell engineering experiments, tissue culture devices for seed and seedling cultivation can be applied to the breeding and cultivation of various plants. These devices can precisely control conditions such as nutrition, light, temperature, and humidity by simulating an ideal growth environment, thereby enabling the efficient cultivation of various plant seedlings. Different plants have different environmental requirements, but most tissue culture systems can adapt to a variety of common agricultural and horticultural plants.

[0003] By using cell engineering and tissue culture techniques to cultivate potato seedlings, goals such as effective asexual reproduction, variety improvement, and enhanced disease resistance can be achieved. In a tissue culture device, the cultivation of potatoes generally starts from their tuber tissues (such as buds or stem segments) to cultivate pathogen-free healthy seedlings.

[0004] In existing tissue culture devices for cell engineering experiments, during the process of cultivating potato seedlings, the operation of manually cutting potato tubers from potatoes may have the following several disadvantages: High labor intensity: Manual operation may lead to uneven cutting, with some tubers being cut too thin or too thick. This will not only affect their subsequent growth and development, but improper cutting may also cause damage to the potato tubers, thereby affecting the effect of tissue culture.

[0005] Risk of contamination: During the manual operation process, the hands are prone to carrying bacteria or other microorganisms, thereby increasing the risk of contamination. Even with hand disinfection, it is difficult to completely prevent external contamination, which is very likely to reduce the success rate of tissue culture.

[0006] In summary, in the prior art, there is a lack of technology that can automatically cut potato tubers during the process of cultivating potato seedlings. Summary of the Invention

[0007] The purpose of the present invention is to solve the disadvantages existing in the background art and to propose a tissue culture device for cell engineering experiments.

[0008] To achieve the above purpose, the technical solution adopted by the present invention is as follows: A tissue culture device for cell engineering experiments, including a culture box, a treatment box is fixedly connected to one side of the culture box, a top frame is slidably fitted to the inner wall of the treatment box, a potato placement rack is fixedly connected to the lower side of the top frame, a moving seat is slidably fitted to one side of the potato placement rack, a potato stem block separation mechanism is rotatably connected to the moving seat, a transmission mechanism is rotatably connected to one side inner wall of the treatment box, and a receiving groove is slidably fitted to one side inner wall of the treatment box.

[0009] Preferably, a box door is rotatably connected to the front side of the processing box, a valve is fixedly installed at the bottom opening of the processing box, a collection seat is movably inserted at the opening of the processing box located at the material receiving groove, and a camera is fixedly installed on one side of the processing box.

[0010] Preferably, potatoes are placed on one side of the potato placement rack. A motor A is fixedly connected to one side of the potato placement rack. A positioning plate is slidably fitted to the output end of the motor A. A plurality of steel needles are fixedly connected to the lower surface of the positioning plate. A pressure applying frame is rotatably connected to the outer wall of the positioning plate. The other end of the pressure applying frame is slidably fitted to the inner wall of the processing box. A spring is fixedly connected to the pressure applying frame, and the other end of the spring is fixedly connected to the inner wall of the processing box.

[0011] Preferably, a motor B is fixedly connected to the other side of the potato placement rack. A threaded rod is fixedly connected to the output end of the motor B. The threaded rod is threadedly connected to the moving seat.

[0012] Preferably, an electric push rod A is fixedly connected to one side of the moving seat. An adjusting rack is fixedly connected to the output end of the electric push rod A. A driving rack A and a driving rack B are respectively fixedly connected to one side of the moving seat.

[0013] Preferably, the potato stem and block separating mechanism includes a rotating seat. The rotating seat is rotatably connected to the moving seat. One end of the rotating seat is fixedly connected to an adjusting wheel. The adjusting wheel is meshed and driven with the adjusting rack. An electric push rod B is fixedly connected to the rotating seat. The output end of the electric push rod B is fixedly connected to a U-shaped frame.

[0014] Preferably, a semi-circular cutter is rotatably connected to the U-shaped frame. One end of the semi-circular cutter is fixedly connected to a motor C. The motor C is fixedly connected to the U-shaped frame. A pushing rod is slidably fitted to one side of the U-shaped frame. One end of the pushing rod is slidably fitted to the inner wall of the semi-circular cutter. The other side of the pushing rod is in movable contact with the rotating seat. A tension spring is fixedly connected to the pushing rod, and the other end of the tension spring is fixedly connected to the U-shaped frame.

[0015] Preferably, the transmission mechanism includes a universal joint. The universal joint is rotatably connected to the potato placement rack. One end of the universal joint is fixedly connected to a driving wheel A. The driving wheel A is meshed and driven with the driving rack A. The other end of the universal joint is fixedly connected to a worm. A worm gear is meshed and driven on one side of the worm. A connecting rod group is fixedly connected to one side of the worm gear. Both ends of the connecting rod group are respectively rotatably connected to the processing box and the top frame.

[0016] Preferably, an inclined surface is provided on the inner wall of the material receiving groove. One side of the material receiving groove is fixedly connected with a grooved rod. A rotating rod is slidably fitted inside the grooved rod. The other end of the rotating rod is rotatably connected to the inner wall of the treatment box. A transmission wheel B is fixedly connected to the end of the rotating rod connected to the treatment box. The transmission wheel B is in meshing transmission with a transmission rack B.

[0017] Compared with the prior art, the present invention has the following beneficial effects: 1. By providing a rotatable positioning plate and a potato stem block separating mechanism, after the positioning plate fixes the potato and drives it to rotate, using the rotatable semi-circular cutter, the stem blocks at different positions on the potato can be automatically cut off, avoiding problems such as uneven cutting, too deep or too shallow cutting in manual operation, significantly improving the cutting efficiency, accuracy and consistency, and reducing the interference of human factors. At the same time, the automated operation can reduce the risk of contamination caused by human factors. Especially in the aseptic operation experiment of cell engineering, machine operation is more hygienically reliable than manual operation. In addition, the reduction of the contact opportunity between the operator and the plant material further reduces the possibility of microbial contamination.

[0018] 2. By providing a transmission mechanism, after the semi-circular cutter cuts off a potato stem block, the threaded rod drives the moving seat to move to the lowest side, and then drives the transmission mechanism to move the top frame downward, so that the potato and the semi-circular cutter are immersed in the hydrogen peroxide solution at the bottom of the treatment box for automatic disinfection treatment. The operator does not need to manually operate the disinfection process of the potato and the semi-circular cutter, which not only saves labor costs and time, but also significantly improves the efficiency, uniformity and safety of potato disinfection.

[0019] 3. By providing a push rod and a material receiving groove, the potato stem block in the semi-circular cutter can be automatically pushed out and the cut stem block can be automatically received, avoiding the direct contact between the operator and the cut stem block, and effectively reducing the risk of cross-contamination. Especially in operations with high requirements for a clean environment, this setting can effectively reduce the adverse effects of external contamination on the potato stem block and the subsequent experimental process. BRIEF DESCRIPTION OF THE DRAWINGS

[0020] Figure 1 is a schematic diagram of the overall structure of a tissue culture device for a cell engineering experiment of the present invention; Figure 2 is a schematic diagram of a partial structure of a tissue culture device for a cell engineering experiment of the present invention; Figure 3 is a schematic diagram of a partial cross-section of the structure of the treatment box of a tissue culture device for a cell engineering experiment of the present invention; Figure 4 is a schematic diagram of the structure of the potato placement rack of a tissue culture device for a cell engineering experiment of the present invention; Figure 5Schematic diagram of the moving base and other structures of a tissue culture device for a cell engineering experiment of the present invention; Figure 6 Schematic diagram of the potato stem block separation mechanism of a tissue culture device for a cell engineering experiment of the present invention; Figure 7 Schematic diagram of the transmission mechanism and other structures of a tissue culture device for a cell engineering experiment of the present invention; Figure 8 Schematic diagram of the material receiving trough structure of a tissue culture device for a cell engineering experiment of the present invention.

[0021] The labels in the figure are: 1. Incubator; 2. Processing box; 3. Top frame; 4. Potato placement rack; 5. Moving base; 6. Potato stem block separation mechanism; 7. Transmission mechanism; 8. Material receiving trough; 201. Valve; 202. Collection base; 401. Motor A; 402. Positioning plate; 403. Pressing frame; 404. Spring; 405. Motor B; 406. Threaded rod; 501. Electric push rod A; 502. Adjusting rack; 503. Transmission rack A; 504. Transmission rack B; 601. Rotating seat; 602. Adjusting wheel; 603. Electric push rod B; 604. U-shaped frame; 605. Semi-circular cutter; 606. Motor C; 607. Pushing rod; 608. Tension spring; 701. Universal joint; 702. Driving wheel A; 703. Worm; 704. Worm gear; 705. Link group; 801. Grooved rod; 802. Rotating rod; 803. Driving wheel B. Detailed implementation manners

[0022] The following description is used to disclose the present invention so that those skilled in the art can implement the present invention. The preferred embodiments described below are only examples, and those skilled in the art can think of other obvious variations.

[0023] As Figures 1-8 shown, a tissue culture device for a cell engineering experiment includes an incubator 1. A processing box 2 is fixedly connected to one side of the incubator 1. A top frame 3 is slidably fitted to the inner wall of the processing box 2. A potato placement rack 4 is fixedly connected to the lower side of the top frame 3. A moving base 5 is slidably fitted to one side of the potato placement rack 4. A potato stem block separation mechanism 6 is rotatably connected to the moving base 5. A transmission mechanism 7 is rotatably connected to the inner wall of one side of the processing box 2. A material receiving trough 8 is slidably fitted to the inner wall of one side of the processing box 2.

[0024] As Figure 3 shown, a box door is rotatably connected to the front side of the processing box 2. A valve 201 is fixedly installed at the bottom opening of the processing box 2. A collection base 202 is movably inserted at the opening of the processing box 2 corresponding to the material receiving trough 8. A camera is fixedly installed on one side of the processing box 2.

[0025] As Figure 4As shown, potatoes are placed on one side of the potato rack 4. On one side of the potato rack 4, a motor A401 is fixedly connected. The output end of the motor A401 is slidably fitted with a positioning plate 402. A plurality of steel needles are fixedly connected to the lower surface of the positioning plate 402. The outer wall of the positioning plate 402 is rotatably connected with a pressure-applying frame 403. The other end of the pressure-applying frame 403 is slidably fitted with the inner wall of the treatment box 2. A spring 404 is fixedly connected to the pressure-applying frame 403, and the other end of the spring 404 is fixedly connected to the inner wall of the treatment box 2. Lift the positioning plate 402, place the disinfected potatoes on the potato rack 4, and then press down and release the positioning plate 402 so that the steel needles on the positioning plate 402 can be inserted into the potatoes.

[0026] On the other side of the potato rack 4, a motor B405 is fixedly connected. The output end of the motor B405 is fixedly connected with a threaded rod 406, and the threaded rod 406 is threadedly connected with the moving seat 5. Drive the connected threaded rod 406 to rotate by using the motor B405, so that the threaded rod 406 drives the moving seat 5 to move to a suitable position.

[0027] As Figure 5 shown, an electric push rod A501 is fixedly connected to one side of the moving seat 5. The output end of the electric push rod A501 is fixedly connected with an adjusting rack 502. A driving rack A503 and a driving rack B504 are respectively fixedly connected to one side of the moving seat 5. Drive the adjusting rack 502 to move by using the electric push rod A501, so that the adjusting rack 502 drives the rotating seat 601 connected to the adjusting wheel 602 to rotate to a suitable angle.

[0028] As Figure 6 shown, the potato stem and tuber separation mechanism 6 includes a rotating seat 601. The rotating seat 601 is rotatably connected with the moving seat 5. One end of the rotating seat 601 is fixedly connected with an adjusting wheel 602. The adjusting wheel 602 is meshed and driven with the adjusting rack 502. An electric push rod B603 is fixedly connected to the rotating seat 601, and the output end of the electric push rod B603 is fixedly connected with a U-shaped frame 604.

[0029] A semi-circular cutter 605 is rotatably connected to the U-shaped frame 604. One end of the semi-circular cutter 605 is fixedly connected to a motor C 606, and the motor C 606 is fixedly connected to the U-shaped frame 604. A push rod 607 is slidably fitted on one side of the U-shaped frame 604. One end of the push rod 607 is slidably fitted with the inner wall of the semi-circular cutter 605. The other side of the push rod 607 is in movable contact with the rotating seat 601. A tension spring 608 is fixedly connected to the push rod 607, and the other end of the tension spring 608 is fixedly connected to the U-shaped frame 604. The tension spring 608 realizes the automatic reset of the push rod 607. The motor C 606 drives the connected semi-circular cutter 605 to rotate, so that the semi-circular cutter 605 can cut off the potato stem blocks with bud points. Then, when the electric push rod B 603 drives the semi-circular cutter 605 to retract, when one end of the push rod 607 contacts the rotating seat 601, it will drive the push rod 607 to move, so that the push rod 607 is inserted into the semi-circular cutter 605 to push down the potato stem blocks.

[0030] By setting the rotatable positioning plate 402 and the potato stem block separation mechanism 6, the positioning plate 402 fixes the potato and drives it to rotate. Using the rotatable semi-circular cutter 605, the stem blocks at different positions on the potato can be automatically cut off, avoiding problems such as uneven cutting, too deep or too shallow cutting in manual operation, significantly improving the cutting efficiency, accuracy and consistency, and reducing the interference of human factors. At the same time, automated operation can reduce the risk of contamination caused by human factors. Especially in the aseptic operation experiment of cell engineering, machine operation is more hygienically reliable than manual operation. In addition, the reduction of the contact opportunity between the operator and the plant material further reduces the possibility of microbial contamination.

[0031] As Figure 7 shown, the transmission mechanism 7 includes a universal joint 701. The universal joint 701 is rotatably connected to the potato placement rack 4. One end of the universal joint 701 is fixedly connected to a transmission wheel A 702, and the transmission wheel A 702 is meshed and transmitted with the transmission rack A 503. The other end of the universal joint 701 is fixedly connected to a worm 703. One side of the worm 703 is meshed and transmitted with a worm gear 704. One side of the worm gear 704 is fixedly connected to a connecting rod group 705. Both ends of the connecting rod group 705 are respectively rotatably connected to the processing box 2 and the top frame 3. The transmission rack A 503 on the moving seat 5 will drive the transmission wheel A 702 to rotate, so that the transmission wheel A 702 drives the worm 703 connected by the universal joint 701 to rotate, so that the worm 703 can drive the connecting rod group 705 connected by the worm gear 704 to rotate. At this time, the connecting rod group 705 will drive the top frame 3 to move down.

[0032] As Figure 8As shown in the figure, the inner wall of the material receiving groove 8 is provided with an inclined surface. One side of the material receiving groove 8 is fixedly connected with a grooved rod 801. A rotating rod 802 is slidably fitted inside the grooved rod 801. The other end of the rotating rod 802 is rotatably connected to the inner wall of the processing box 2. A transmission wheel B803 is fixedly connected to the end of the rotating rod 802 connected to the processing box 2. The transmission wheel B803 is meshed and driven with a transmission rack B504. The transmission rack B504 on the moving seat 5 drives the rotating rod 802 connected to the transmission wheel B803 to rotate, so that the other end of the rotating rod 802 slides inside the grooved rod 801, thereby pushing the material receiving groove 8 connected to the grooved rod 801 to move away.

[0033] Working principle: Before cultivating potato seedlings, it is necessary to cut the potato stem blocks with bud points from the potatoes for cultivation. First, pour an appropriate amount of hydrogen peroxide solution into the processing box 2, then lift the positioning plate 402, place the disinfected potatoes on the potato placement rack 4, and then press down and release the positioning plate 402 so that the steel needles on the positioning plate 402 can be inserted into the potatoes. Use a camera to collect potato images, and identify the position of the bud points through image processing technology, and then control the device to perform precise cutting, which can realize automatic cutting of potato bud points; First, use the motor A401 to drive the connected positioning plate 402 to rotate, so that the positioning plate 402 drives the potato to rotate, so that the position of the bud point of the potato rotates to one side of the potato stem block separating mechanism 6. Then, adjust the position and angle of the potato stem block separating mechanism 6. By using the motor B405 to drive the connected threaded rod 406 to rotate, the threaded rod 406 drives the moving seat 5 to move to a suitable position. Then, use the electric push rod A501 to drive the adjusting rack 502 to move, so that the adjusting rack 502 drives the rotating seat 601 connected to the adjusting wheel 602 to rotate to a suitable angle. Then, use the electric push rod B603 to drive the U-shaped frame 604 to move, so that the semi-circular cutter 605 approaches the bud point position of the potato; Then, use the motor C606 to drive the connected semi-circular cutter 605 to rotate, so that the semi-circular cutter 605 can cut off the potato stem block with a bud point. Then, when the electric push rod B603 drives the semi-circular cutter 605 to retract, when one end of the push rod 607 contacts the rotating seat 601, it will drive the push rod 607 to move, so that the push rod 607 is inserted into the semi-circular cutter 605 to push the potato stem block down, and it falls into the material receiving groove 8, and then falls into the collection seat 202 through the material receiving groove 8; After cutting off one potato stem block, use the motor B405 to drive the threaded rod 406 to rotate, so that the threaded rod 406 drives the moving seat 5 to move to the lowest side. At this time, the transmission rack B504 on the moving seat 5 drives the rotating rod 802 connected to the transmission wheel B803 to rotate, so that the other end of the rotating rod 802 slides inside the grooved rod 801, thereby pushing the material receiving groove 8 connected to the grooved rod 801 to move away; Then, when the moving seat 5 continues to move downward, the driving rack A503 on the moving seat 5 will drive the driving wheel A702 to rotate, so that the driving wheel A702 drives the worm 703 connected by the universal joint 701 to rotate, enabling the worm 703 to drive the connecting rod group 705 connected to the worm gear 704 to rotate. At this time, the connecting rod group 705 will drive the top frame 3 to move downward, allowing the potato and the semi-circular cutter 605 to be immersed in the hydrogen peroxide solution for disinfection, facilitating the cutting of the next potato stem block.

[0034] It should be noted that in this article, relational terms such as first and second are only used to distinguish one entity or operation from another entity or operation, and do not necessarily require or imply any actual relationship or order between these entities or operations. Moreover, the term "comprising", "including" or any other variation thereof is intended to cover non-exclusive inclusion, so that a process, method, article or device comprising a series of elements not only includes those elements, but also includes other elements not expressly listed, or elements inherent to such process, method, article or device.

[0035] The above shows and describes the basic principles, main features and advantages of the present invention. Those skilled in the art should understand that the present invention is not limited by the above embodiments. What is described in the above embodiments and the specification is only the principle of the present invention. Without departing from the spirit and scope of the present invention, the present invention will have various changes and improvements, and these changes and improvements all fall within the scope of the present invention claimed. The scope of protection claimed by the present invention is defined by the appended claims and their equivalents.

Claims

1. A tissue culture device for cell engineering experiments, comprising an incubator (1), characterized in that: A processing box (2) is fixedly connected to one side of the incubator (1), a top frame (3) is slidably provided on the inner wall of the processing box (2), a potato placement rack (4) is fixedly connected to the lower side of the top frame (3), a moving seat (5) is slidably provided on one side of the potato placement rack (4), a potato tuber separation mechanism (6) is rotatably provided on the moving seat (5), a transmission mechanism (7) is rotatably provided on the inner wall of one side of the processing box (2), and a material receiving trough (8) is slidably provided on the inner wall of one side of the processing box (2).

2. The tissue culture device for a cell engineering experiment according to claim 1, wherein: The front side of the processing box (2) is provided with a box door which is rotatably connected, the bottom opening of the processing box (2) is provided with a valve (201) which is fixedly installed, the opening of the processing box (2) located at the material receiving trough (8) is provided with a collecting seat (202) which is movably plugged, and one side of the processing box (2) is provided with a camera which is fixedly installed.

3. The tissue culture device for a cell engineering experiment according to claim 1, characterized in that: Potatoes are placed on one side of the potato placement rack (4); a motor A (401) is fixedly connected to one side of the potato placement rack (4); a positioning plate (402) is slidably provided at the output end of the motor A (401); a plurality of steel needles are fixedly provided on the lower surface of the positioning plate (402); a pressure rack (403) is rotatably provided on the outer wall of the positioning plate (402); the other end of the pressure rack (403) is slidably provided with the inner wall of the processing box (2); a spring (404) is fixedly provided on the pressure rack (403); the other end of the spring (404) is fixedly provided with the inner wall of the processing box (2).

4. The tissue culture device for a cell engineering experiment according to claim 1, characterized in that: A motor B (405) is fixedly connected to the other side of the potato placement rack (4), a threaded rod (406) is fixedly connected to the output end of the motor B (405), and the threaded rod (406) is threadedly connected to the movable seat (5).

5. The tissue culture device for a cell engineering experiment according to claim 1, wherein: An electric push rod A (501) is fixedly connected to one side of the movable seat (5), an adjustment rack (502) is fixedly connected to the output end of the electric push rod A (501), and a transmission rack A (503) and a transmission rack B (504) are respectively fixedly connected to one side of the movable seat (5).

6. The tissue culture device for cell engineering experiments according to claim 5, wherein: The potato tuber separation mechanism (6) comprises a rotating seat (601), the rotating seat (601) being rotatably connected to the moving seat (5), an adjusting wheel (602) being fixedly connected to one end of the rotating seat (601), the adjusting wheel (602) being meshed and transmission-arranged with an adjusting rack (502), an electric push rod B (603) being fixedly connected to the rotating seat (601), and a U-shaped frame (604) being fixedly connected to the output end of the electric push rod B (603).

7. The tissue culture device for cell engineering experiments according to claim 6, characterized in that: A semi-circular cutter (605) is rotatably connected to the U-shaped frame (604). One end of the semi-circular cutter (605) is fixedly connected to a motor C (606), and the motor C (606) is fixedly connected to the U-shaped frame (604). A push rod (607) is slidably fitted on one side of the U-shaped frame (604). One end of the push rod (607) is slidably fitted with the inner wall of the semi-circular cutter (605), and the other side of the push rod (607) is in movable contact with the rotating seat (601). A tension spring (608) is fixedly connected to the push rod (607), and the other end of the tension spring (608) is fixedly connected to the U-shaped frame (604).

8. A tissue culture device for cell engineering experiments according to claim 7, characterized in that: The transmission mechanism (7) includes a universal joint (701). The universal joint (701) is rotatably connected to the potato placement rack (4). One end of the universal joint (701) is fixedly connected to a transmission wheel A (702), and the transmission wheel A (702) is meshed and driven with the transmission rack A (503). The other end of the universal joint (701) is fixedly connected to a worm (703). A worm gear (704) is meshed and driven on one side of the worm (703). One side of the worm gear (704) is fixedly connected to a connecting rod group (705), and both ends of the connecting rod group (705) are respectively rotatably connected to the processing box (2) and the top frame (3).

9. The tissue culture device for a cell engineering experiment according to claim 8, wherein: The inner wall of the material receiving groove (8) is provided with an inclined surface. A grooving rod (801) is fixedly connected to one side of the material receiving groove (8). A rotating rod (802) is slidably fitted in the inner wall of the grooving rod (801). The other end of the rotating rod (802) is rotatably connected to the inner wall of the processing box (2). A transmission wheel B (803) is fixedly connected to the end of the rotating rod (802) connected to the processing box (2), and the transmission wheel B (803) is meshed and driven with the transmission rack B (504).

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