Application of brassinolide in relieving phytotoxicity of glyphosate to rice and preparation

Abstract

The invention relates to the technical field of crop disease control, in particular to application of brassinolide in relieving phytotoxicity of glyphosate to rice and a preparation. The invention discloses application of brassinolide in relieving phytotoxicity of glyphosate to rice. Brassinolide can effectively relieve the phytotoxicity effect of glyphosate herbicides on rice by promoting cell division and proliferation and accelerating plant physiological recovery. As a natural product, brassinolide has the characteristics of no toxicity and environmental protection, is safe and harmless to a human body, and is convenient to use. The application field of brassinolide is expanded, cognition of functional characteristics of brassinolide in the scientific field is enriched, new technical support is provided for sustainable development of agricultural production, and particularly, the brassinolide has important application value in the aspect of relieving phytotoxicity of glyphosate herbicides to rice, and has wide application prospects. And a new solution is provided for solving practical problems in agricultural production.

Description

Technical Field

[0001] The present invention relates to the technical field of crop disease control, in particular to the application and preparation of brassinolide in alleviating the phytotoxicity of glyphosate to rice. Background Art

[0002] Glyphosate is a broad-spectrum herbicide widely used in the control of farmland weeds. However, while glyphosate controls weeds, it may also cause phytotoxicity to non-target crops. Especially in cases of misuse or drift, it can lead to stunted growth, reduced yield, or even death of rice, seriously affecting the yield and quality of rice. Currently, the methods for alleviating glyphosate phytotoxicity are limited and the effects are not significant. Therefore, it is of great significance to develop an efficient and safe method for alleviating glyphosate phytotoxicity.

[0003] Brassinosteroids (BR) are a kind of highly efficient and broad-spectrum sterol plant hormones, which have various physiological functions such as promoting plant growth, enhancing stress resistance, and improving photosynthesis efficiency. BR is non-toxic and environmentally friendly, which helps it to cope with more adverse environmental conditions without affecting the balance of the ecosystem. In recent years, it has been found that brassinolide has a significant effect on alleviating the negative effects of environmental stresses (such as drought, salt stress, heavy metal toxicity, etc.) on crops. However, there is no report on the application of brassinolide in alleviating the phytotoxicity of glyphosate herbicide to rice. Summary of the Invention

[0004] To solve the above problems, the present invention provides the application and preparation of brassinolide in alleviating the phytotoxicity of glyphosate to rice. By applying brassinolide, the present invention can effectively reduce the phytotoxicity of glyphosate to rice and promote the recovery growth of crops.

[0005] To achieve the above object, the present invention provides the following technical solutions:

[0006] The present invention provides the application of brassinolide in alleviating the phytotoxicity of glyphosate to rice.

[0007] Preferably, the usage concentration of the brassinolide is 0.0001 - 0.005 nmol / L.

[0008] Preferably, the usage concentration of the brassinolide is 0.0005 - 0.001 nmol / L.

[0009] Preferably, the brassinolide promotes cell division and proliferation of rice to alleviate glyphosate phytotoxicity.

[0010] Preferably, the brassinolide enhances the physiological metabolic activity of rice to alleviate glyphosate phytotoxicity.

[0011] The present invention also provides a preparation for alleviating the phytotoxicity of glyphosate to rice, and the preparation contains brassinolide.

[0012] Preferably, the concentration of brassinolide in the preparation is 0.0001 - 0.005 nmol / L.

[0013] Preferably, the concentration of brassinolide in the preparation is 0.0005 - 0.001 nmol / L.

[0014] Advantages of the present invention:

[0015] Brassinolide can effectively alleviate the phytotoxicity of glyphosate herbicide to rice by promoting cell division and proliferation and accelerating the physiological recovery of plants. As a natural product, brassinolide has the characteristics of being non-toxic, green and environmentally friendly, being safe for humans and convenient to use. The present invention expands the application field of brassinolide, not only enriches the scientific community's understanding of its functional characteristics, but also provides new technical support for the sustainable development of agricultural production. Especially, it has important application value in alleviating the phytotoxicity of glyphosate herbicide to crops, and provides a new solution for solving practical problems in agricultural production.

[0016] Application time: Spraying after spraying the glyphosate solution (before the leaves are completely dry) can significantly alleviate the phytotoxicity of glyphosate herbicide to rice, and can minimize the adverse effects of glyphosate on the growth of rice. Description of the drawings

[0017] Figure 1 For the plant height inhibition rate and fresh weight inhibition rate of Examples 1 - 4 and Comparative Examples 1 - 5;

[0018] Figure 2 For the influence of different concentrations of brassinolide on alleviating the phytotoxicity of glyphosate to rice. Detailed implementation manners

[0019] The present invention provides the application of brassinolide in alleviating the phytotoxicity of glyphosate to rice. In the present invention, the use concentration of brassinolide is preferably 0.0001 - 0.005 nmol / L. In the present invention, the use concentration of brassinolide is more preferably 0.0005 - 0.001 nmol / L. In the present invention, brassinolide preferably promotes cell division and proliferation of rice to alleviate the phytotoxicity of glyphosate. In the present invention, brassinolide preferably enhances the physiological metabolic activity of rice to alleviate the phytotoxicity of glyphosate.

[0020] The present invention also provides a preparation for alleviating the phytotoxicity of glyphosate to rice, and the preparation contains brassinolide. In the present invention, the concentration of brassinolide in the preparation is preferably 0.0001 - 0.005 nmol / L. In the present invention, the concentration of brassinolide in the preparation is more preferably 0.0005 - 0.001 nmol / L. The present invention has no special limitations on the dosage form and preparation method of the preparation, etc., and the conventional ones can be adopted.

[0021] To further illustrate the present invention, the present invention will be described in detail below in conjunction with embodiments, but they cannot be understood as limiting the protection scope of the present invention.

[0022] Example 1

[0023] Crop: Rice

[0024] Experimental method: Weigh an appropriate amount of brassinolide accurately using an analytical balance (accurate to 0.0001 g). First, prepare a stock solution of 10 nmol / L with distilled water, and then dilute it with distilled water to obtain a spraying preparation. The concentration of brassinolide in the spraying preparation is 0.0001 nmol / L. Using clear water as the control group (CK), when the concentration of brassinolide is 0, it means that only the glyphosate solution is sprayed.

[0025] Cultivate rice by the potting method, with one seedling per hole. Select a 32-hole seedling tray with a diameter of 4.5 cm and fill it with nutrient substrate soil to 4 / 5 of the hole capacity. Sow the germinated rice seeds directly into the substrate and cover the surface with an appropriate amount of soil. Use the bottom infiltration method to make the substrate fully absorb water and maintain suitable water and fertilizer conditions. Wait until the rice seedlings grow to the 3 - 4 leaf stage and then set aside. Set up a treatment group and a control group in the experiment, with 3 replicates (3 trays) in each group. The treatment group uniformly sprays a glyphosate solution of 1080 g a.i. / hm 2 When the leaves are wet and not dry on the surface, immediately spray brassinolide solutions with different concentration gradients. The spraying operation is carried out using a 3WP - 2000 type automatic spray tower (developed by Nanjing Institute of Agricultural Mechanization) to ensure that the liquid medicine is evenly distributed on the rice leaf surface.

[0026] Investigate the inhibition rate (%) of glyphosate on the plant height and fresh weight of rice 21 days after applying the medicine. The calculation method of the inhibition rate is:

[0027]

[0028] Among them, I represents the inhibition rate of glyphosate on the plant height (or fresh weight of the above-ground part) of rice (unit: %), CK represents the plant height (or fresh weight of the above-ground part) of rice in the clear water control group, unit: cm (or g), and S represents the plant height (or fresh weight of the above-ground part) of rice under different treatments, unit: cm (or g).

[0029] Example 2

[0030] The difference from Example 1 is that the concentration of brassinolide in the spraying preparation is 0.0005 nmol / L, and the rest is the same as in Example 1.

[0031] Example 3

[0032] The difference from Example 1 is that the concentration of brassinolide in the spraying preparation is 0.001 nmol / L, and the rest is the same as in Example 1.

[0033] Example 4

[0034] The difference from Example 1 is that the concentration of brassinolide in the spraying preparation is 0.005 nmol / L, and the rest is the same as in Example 1.

[0035] Comparative Example 1

[0036] The difference from Example 1 is that the concentration of brassinolide in the spraying preparation is 0.01 nmol / L, and the rest is the same as in Example 1.

[0037] Comparative Example 2

[0038] The difference from Example 1 is that the concentration of brassinolide in the spraying preparation is 0.05 nmol / L, and the rest is the same as in Example 1.

[0039] Comparative Example 3

[0040] The difference from Example 1 is that the concentration of brassinolide in the spraying preparation is 0.1 nmol / L, and the rest is the same as in Example 1.

[0041] Comparative Example 4

[0042] The difference from Example 1 is that the concentration of brassinolide in the spraying preparation is 0.5 nmol / L, and the rest is the same as in Example 1.

[0043] Comparative Example 5

[0044] The difference from Example 1 is that the concentration of brassinolide in the spraying preparation is 1 nmol / L, and the rest is the same as in Example 1.

[0045] The biological results of Examples 1 to 4 and Comparative Examples 1 to 5 are as follows:

[0046] The rice seedlings sprayed only with glyphosate showed obvious leaf chlorosis and death.

[0047] The rice seedlings treated with glyphosate + brassinolide are related to the concentration of brassinolide sprayed. When the concentration of brassinolide is in the range of 0.0001 - 0.005 nmol / L, the leaf yellowing phenomenon is significantly alleviated, and the plant height inhibition rate and fresh weight inhibition rate are significantly lower than those of the treatment with only glyphosate sprayed.

[0048] Although the above embodiments have described the present invention in detail, they are only a part of the embodiments of the present invention, rather than all embodiments. People can also obtain other embodiments based on these embodiments without creative efforts, and these embodiments all fall within the protection scope of the present invention.

Claims

1. Application of brassinolide in alleviating phytotoxicity of glyphosate to rice.

2. The application according to claim 1, wherein The application concentration of the brassinolide is 0.0001 - 0.005 nmol / L.

3. The application according to claim 1, wherein The application concentration of the brassinolide is 0.0005 - 0.001 nmol / L.

4. The application according to claim 1, wherein The brassinolide promotes cell division and proliferation of rice to alleviate glyphosate phytotoxicity.

5. The application according to claim 1, characterized in that, The brassinolide enhances the physiological metabolic activity of rice to alleviate glyphosate phytotoxicity.

6. A preparation for alleviating the phytotoxicity of glyphosate to crops, characterized in that, The preparation contains brassinolide.

7. The preparation according to claim 6, characterized in that, The concentration of brassinolide in the preparation is 0.0001 - 0.005 nmol / L.

8. The preparation according to claim 6 or 7, characterized in that, The concentration of brassinolide in the preparation is 0.0005 - 0.001 nmol / L.

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