A mixed additive for increasing intramuscular fat content of pigs, application and pig feed

Abstract

The application belongs to the field of feed and discloses a mixed additive for increasing intramuscular fat content of pigs, which comprises black soldier fly super-micro powder, Chinese herbal medicine super-micro powder and compound amino acid; the Chinese herbal medicine super-micro powder is obtained by super-micro grinding of radix astragali, honeysuckle and eucommia; and the black soldier fly super-micro powder is obtained by super-micro grinding of black soldier fly dry insects. The additive is combined with the black soldier fly super-micro powder, the Chinese herbal medicine super-micro powder and the compound amino acid, so that the intramuscular fat content of pigs can be significantly increased. In addition, the application also discloses application of the additive and pig feed.

Description

TECHNICAL FIELD

[0001] The present application relates to the field of feed, in particular to a mixed additive for increasing intramuscular fat content of pigs, application and pig feed. BACKGROUND

[0002] Black soldier fly has been widely used as a feed additive. The present unit has conducted research on black soldier fly for many years, and the following applications are available:

[0003] Patent application with publication number CN109497103A, subjecting to a composite lipid, reducing odor and odor emission of livestock and poultry manure, composite lipid spray and its preparation method and application;

[0004] Patent application with publication number CN112868938A, subjecting to a biological agent and its preparation method;

[0005] Patent application with publication number CN109674778A, subjecting to a lipid antibacterial composition, extraction method, detection method and use;

[0006] Patent application with publication number CN113493288B, subjecting to a composition for fermentation deodorization of livestock and poultry breeding manure and its preparation method;

[0007] Patent application with publication number CN115299526B, subjecting to a piglet feed;

[0008] Among them, in the patent application with publication number CN115299526B, subjecting to a piglet feed, the contribution of black soldier fly oil extract to the growth performance and immune performance of piglets is discussed.

[0009] The purpose of the present project is to explore the related application of black soldier fly in finishing pigs. SUMMARY

[0010] The purpose of the present application is to provide a mixed additive for increasing intramuscular fat content of pigs, which uses black soldier fly ultrafine powder, Chinese herbal medicine ultrafine powder and composite amino acid in combination, so as to significantly increase the intramuscular fat content of pigs.

[0011] The specific scheme of the present application is as follows:

[0012] A mixed additive for increasing intramuscular fat content of pigs, comprising black soldier fly ultrafine powder, Chinese herbal medicine ultrafine powder and composite amino acid.

[0013] The Chinese herbal medicine ultrafine powder is obtained by grinding radix astragali, honeysuckle and eucommia through an ultrafine grinding process;

[0014] The black soldier fly ultrafine powder is obtained by ultrafine grinding of black soldier fly dry worms.

[0015] The internal mechanism of the composition for significantly improving the intramuscular fat content of pigs is as follows: the conjugated linoleic acid (CLA, cis-9, trans-11 and trans-10, cis-12 isomers) in the Hermetia illucens ultrafine powder activates the PPARγ signaling pathway, up-regulates the expression of fatty acid synthase (FAS) and acetyl-CoA carboxylase (ACC), promotes adipocyte differentiation and lipid deposition; at the same time, the chitin degradation product N-acetylglucosamine and the branched chain amino acids (leucine, isoleucine) in the complex amino acid synergistically activate the mTORC1 pathway, enhance the expression of lipid droplet coating protein (PLIN2), and promote the secretion of adiponectin. The astragalus polysaccharide and the green acid of Eucommia ulmoides in the Chinese herbal medicine ultrafine powder regulate the AMPK / mTOR dual pathways, promote glucose uptake (astragaloside A) and guide triglyceride synthesis (PEPCK inhibition by Eucommia ulmoides flavones), and the iridoid of Lonicera japonica optimizes the efficiency of mitochondrial beta-oxidation and balances energy metabolism. Under the synergistic action of the three, the short-chain fatty acids (SCFAs) produced by the metabolism of intestinal flora trigger the muscle lipid accumulation signal through the GPR41 receptor, and the antioxidant network composed of the Eucommia ulmoides lignan and the flavones of Lonicera japonica significantly reduces the muscle MDA level and maintains the integrity of lipid droplets, so as to finally realize the multi-dimensional synergistic effect of lipid synthesis, energy metabolism and oxidation-reduction system.

[0016] In the above-mentioned mixed additive, the weight ratio of the Hermetia illucens ultrafine powder, the Chinese herbal medicine ultrafine powder and the complex amino acid is 1-3: 1-3: 1-3.

[0017] In the above-mentioned mixed additive, the weight ratio of the Hermetia illucens ultrafine powder, the Chinese herbal medicine ultrafine powder and the complex amino acid is 2: 2-3: 1-3.

[0018] In the above-mentioned mixed additive, the weight ratio of the Hermetia illucens ultrafine powder, the Chinese herbal medicine ultrafine powder and the complex amino acid is 2: 2-3: 1-3.

[0019] In the above-mentioned mixed additive, the composition of each amino acid in the complex amino acid is as follows:

[0020] The ratio of arginine: leucine: isoleucine: valine is 1: 1: 0.75-0.25: 0.75-0.25.

[0021] Meanwhile, the application also discloses the application of the mixed additive as described above in the preparation of pig feed.

[0022] In the above-mentioned application, the pig feed is pig feed for improving the intramuscular fat content of pigs.

[0023] In addition, the application also discloses a pig feed containing the mixed additive as described above.

[0024] The beneficial effects of the present application are:

[0025] The present application adopts super-micro powder of black soldier fly, super-micro powder of Chinese herbal medicine and compound amino acid for combination use, which can significantly improve the intramuscular fat content of pigs; the present application firstly adopts single variable for testing, and selects super-micro powder of black soldier fly, super-micro powder of Chinese herbal medicine and compound amino acid, which can obviously improve the intramuscular fat content of pigs; then, the present application adopts orthogonal experiment to verify the optimal dosage ratio of the three in combination, and meanwhile, it is found in the verification process that when the ratio of the three is 2:1-3:1-3, the increase of the intramuscular fat content of pigs shows synergism, and when the ratio of the three is 2:2-3:1-3, the growth performance shows certain synergism. DETAILED DESCRIPTION

[0026] The present application will be described clearly and completely in combination with the embodiments of the present application, and in the description of the present application, it should be noted that the specific conditions not mentioned in the embodiments are carried out according to conventional conditions or the conditions suggested by the manufacturer. The reagents or instruments not mentioned by the manufacturer are all conventional products that can be purchased in the market.

[0027] Black soldier fly: purchased from (Bio-source Biotechnology (Shenzhen) Co., Ltd.), which is dry worm, and the water content is ≤5%;

[0028] Radix Astragali, honeysuckle and Eucommia ulmoides are decoction pieces.

[0029] The above super-micro powder of black soldier fly and super-micro powder of Chinese herbal medicine are realized by a super-micro grinder and are passed through a 300-mesh sieve.

[0030] First part: screening of efficacy substances

[0031] After the pre-screening in this part, it is found that conjugated linoleic acid, compound amino acid, betaine, black soldier fly and Chinese herbal medicine mixture are potential optional materials, and the black soldier fly and Chinese herbal medicine mixture are used in the form of the above super-micro powder.

[0032] The components of conjugated linoleic acid are cis-9, trans-11-octadecadienoic acid and trans-10, cis-12-octadecadienoic acid.

[0033] The components of compound amino acid are arginine, leucine, isoleucine and valine, and the ratio of the four is 1:1:0.5:0.5.

[0034] The components of Chinese herbal medicine mixture are Radix Astragali, honeysuckle and Eucommia ulmoides, and the weight ratio of the three is 10:3:2.

[0035] Experimental method:

[0036] 1. Select 30 fattening pigs of about 50 kg, weigh and number them;

[0037] 2. Divide the fattening pigs into groups of 5, making a total of 6 groups, and number each group;

[0038] 3. Feed each group with a specific amount (2wt%) of the compound according to the group number, with one group serving as a control group;

[0039] 4. Slaughter after 90 days of feeding. Stop feeding 24 hours before slaughter and stop watering 2 hours before slaughter to measure the live weight before slaughter.

[0040] 5. After slaughter, samples shall be taken and intramuscular fat shall be tested in accordance with 0NY / T 821-2019.

[0041] The basic formula and nutritional components of the feed can be found in Table 1 below;

[0042] Table 1 Feed Composition Table

[0043]

[0044]

[0045] The experimental results can be found in Table 2;

[0046] Table 2 Experimental Results

[0047]

[0048] As shown in Table 2, amino acids, betaine, black soldier fly larvae, and the mixture of traditional Chinese medicine have significant advantages in increasing intramuscular fat content. In terms of growth rate, amino acids, black soldier fly larvae, and the mixture of traditional Chinese medicine also have significant advantages. Based on relevant experiments and our experience with each substance, we selected amino acids, black soldier fly larvae, and the mixture of traditional Chinese medicine for subsequent orthogonal experiments.

[0049] Part Two: Orthogonal Experiments

[0050] The experimental method is the same as in Part 1;

[0051] The relevant parameters for the orthogonal experiment are shown in Table 3;

[0052] The total amount of the composition used in each experimental group was the same, 2 wt%.

[0053] The experimental results are shown in Table 4.

[0054] Table 3. Formula ratios for orthogonal experiments

[0055]

[0056] Table 4. Orthogonal Results

[0057]

[0058]

[0059] Result analysis:

[0060] 1. From the data in Table 4, it can be seen that the orthogonal experimental groups with obvious increase in intramuscular fat content are experimental group 4, experimental group 5, experimental group 6, experimental group 7, experimental group 8 and experimental group 9; from the perspective of average growth rate, the effects of experimental group 2, experimental group 3, experimental group 5, experimental group 6, experimental group 7 and experimental group 8 are more obvious;

[0061] 2. From the data in Table 2 and Table 4, it can be seen that experimental group 4, experimental group 5 and experimental group 6 have obvious synergies in terms of intramuscular fat content; experimental group 5 and experimental group 6 have obvious synergies in terms of average growth rate;

[0062] In summary, the mixed additive of the present case has obvious effects in improving intramuscular fat content and growth rate, and has great market value.

Claims

1. A mixed additive for increasing intramuscular fat content of pigs, characterized in that, The black soldier fly super-micro powder, the Chinese herbal medicine super-micro powder and the compound amino acid; The Chinese herbal medicine super-micro powder is obtained by super-micro grinding of Astragalus membranaceus, honeysuckle and Eucommia ulmoides; The black soldier fly super-micro powder is obtained by super-micro grinding of black soldier fly dry worm; the weight ratio of the black soldier fly super-micro powder, the Chinese herbal medicine super-micro powder and the compound amino acid is 2:2-3:1-3; the weight ratio of Astragalus membranaceus, honeysuckle and Eucommia ulmoides in the Chinese herbal medicine super-micro powder is 10:1-5:1-5; The composition of each amino acid in the compound amino acid is that the ratio of arginine:leucine:isoleucine:valine is 1:1:0.75-0.25:0.75-0.

25.

2. The use of the mixed additive in claim 1 in the preparation of pig feed.

3. Use according to claim 2, characterized in that, The pig feed is pig feed for increasing the intramuscular fat content of pigs.

4. A pig feed, characterized in that, The pig feed contains the mixed additive in claim 1.

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