Feed for promoting intestinal repair of aquatic animals and preparation method thereof

Abstract

The invention relates to the technical field of animal feeds, and particularly provides a feed for promoting intestinal repair of aquatic animals and a preparation method of the feed. Comprising the following raw materials in percentage by mass: 47% of duck blood, 0.5%-1% of vitamin C, 0.1%-0.3% of tea polyphenol, 0.5%-1.5% of saccharomyces cerevisiae, 0.5%-1.5% of lactobacillus plantarum, 4%-6% of beta-glucan, 2%-4% of stachyose, 0.1% of sodium butyrate and 30% of vitamin C, 35% of plant extract and the balance of pure water. According to the feed for promoting intestinal repair of the aquatic animals and the preparation method of the feed, food calling, intestinal motility recovery and intestinal beneficial bacterium density increase are taken into consideration.

Description

Technical Field

[0001] The present application relates to the technical field of animal feed, and in particular to a feed for promoting intestinal repair of aquatic animals and a preparation method thereof. Background Art

[0002] The intestine is the most important organ of aquatic animals. It is responsible for the digestion and metabolism of aquatic animals, ensuring their health and having a great effect on the body's immunity and growth performance. In aquatic animals, in freshwater fish, especially catfish farming, intestinal health plays a very important role in high-quality green farming. However, in recent years, the aquaculture industry has developed rapidly. Many artificial breeds have unilaterally pursued high-density farming and rapid growth, and continuously increased the amount of feed input, which has put a severe burden on the catfish intestine. The intestinal villi have decreased, and the density of beneficial bacteria headed by lipase-secreting yeast and lactic acid-producing lactic acid bacteria has decreased, which has been so serious that enteritis has caused death and polluted water bodies, forming a vicious cycle. To reverse this problem, it is necessary to increase the density of beneficial bacteria in the catfish intestine, reduce the burden on the catfish intestine, and thus re-stimulate the secretion of digestive juices and feeding motivation of catfish.

[0003] Therefore, there is a need for a feed that can increase the density of beneficial bacteria and promote intestinal repair in aquatic animals, and a preparation method thereof. Summary of the invention

[0004] In order to solve the above problems, the present invention aims to provide a feed for promoting intestinal repair of aquatic animals and a preparation method thereof.

[0005] On the one hand, the present application provides a feed for promoting intestinal repair of aquatic animals, wherein the feed for promoting intestinal repair of aquatic animals comprises: a nutrient solution with a mass fraction of 76% and a glue solution to make up the remaining mass fraction; The nutrient solution is uniformly mixed with: 47% duck blood by mass, 0.5-1% vitamin C by mass, 0.1-0.3% tea polyphenols by mass, 0.5-1.5% saccharomyces cerevisiae by mass, 0.5-1.5% plant lactobacillus by mass, 4-6% beta-glucan by mass, 2-4% stachyose by mass, 0.1% sodium butyrate by mass, 30-35% plant extract by mass, and pure water to make up the rest; The glue solution is a uniform mixture of 16% by mass of glycerin, 40% by mass of gelatin, 3-5% by mass of shrimp oil and water to make up the rest. The preparation method of the plant extract is as follows: Take Atractylodes macrocephala with a mass fraction of 28%, Coptis chinensis with a mass fraction of 20 - 25%, Cinnamomum cassia with a mass fraction of 16%, Citrus reticulata Blanco with a mass fraction of 12%, Sophora flavescens with a mass fraction of 8 - 12%, and Glycyrrhiza uralensis Fisch. to make up the remaining mass fraction. Crush them to 10 meshes, add 85% ethanol with a mass 6 times that of the total mass of the above medicinal materials, immerse them at 25°C for 40 h, and filter to obtain a crude filtered extract; add pure water with a mass 0.2 times that of the crude filtered extract to the crude filtered extract, and then perform vacuum distillation at 60°C and a vacuum degree of 0.098 Mpa until the mass is 0.2 times that before evaporation to obtain the plant extract.

[0006] Further, the mass fraction of vitamin C is 0.7%.

[0007] Further, the mass fraction of tea polyphenols is 0.2%.

[0008] Further, the mass fraction of Saccharomyces cerevisiae is 1%.

[0009] Further, the mass fraction of Lactobacillus plantarum is 1.2%.

[0010] Further, the mass fraction of β - glucan is 5%.

[0011] Further, the mass fraction of stachyose is 3%.

[0012] Further, the mass fraction of the plant extract is 33%.

[0013] Further, the mass fraction of shrimp oil is 4%.

[0014] Further, the mass fraction of Coptis chinensis is 23%.

[0015] Further, the mass fraction of Sophora flavescens is 10%.

[0016] On the other hand, the present application also provides a preparation method of a feed for promoting the intestinal repair of aquatic animals as described above: Stir and gelatinize the glue solution at 60°C, then keep it warm at 55°C and connect it to an HSR - 300C soft capsule machine. Set the particle size to 8 mm, and the content is the nutrient solution, with each capsule filled with 800 mm.

[0017] Through the present application, the following beneficial effects can be achieved: The feed for promoting the intestinal repair of aquatic animals and its preparation method according to the present application take into account palatability induction, intestinal repair, anti-inflammation, inhibition of harmful bacteria and increase in the density of beneficial intestinal bacteria. Catfish are bottom-dwelling fish, active at night and with degenerated vision during the day, and they hunt by smell and whiskers; the proportion of gelatin and particle size in the feed of the present application make the feed sink to the bottom and not disintegrate after soaking in pure water at 30 °C for 12 hours before being eaten, which is suitable for feeding catfish; shrimp oil rich in unsaturated fatty acids is added to the gelatin solution in the present application, which plays a role in palatability induction and nutrition, making the feeding consumption rate reach 100%; the present application uses soft capsules to coat the nutrient solution, avoiding consumption such as soaking and rotting, and at the same time protecting the internal nutrient solution, avoiding the loss of the activity of the nutrient solution caused by the plasmid of ordinary feed during high-temperature drying; the gelatin of the gelatin solution in the present application is a natural animal product and the content is a nutrient solution, which is easy for catfish to digest and reduces their digestive burden; the present application uses duck blood with a longer clotting time in the nutrient solution in combination with a specific ratio of vitamin C and tea polyphenols to extend the clotting time and maintain the activity of the nutrient solution by antioxidant; the present application adds the main beneficial bacteria Saccharomyces cerevisiae and Lactobacillus plantarum in fish intestines to the nutrient solution, and in combination with a specific ratio of β-glucan, stachyose and sodium butyrate, promotes the reproduction of Saccharomyces cerevisiae and Lactobacillus plantarum in the intestine, increases the density of beneficial intestinal bacteria, enables the beneficial bacteria to form a dominant flora to compress the living space of harmful bacteria, inhibits harmful bacteria, and repairs the intestinal flora environment; the plant extract in the present application has anti-inflammatory effects on the one hand and avoids the generation of drug resistance, and on the other hand, it nourishes the intestine and promotes the repair of the digestive system. The components of various ratios in the present application and the combined process together achieve the effect of increasing the density of beneficial flora and promoting the intestinal repair of the aquatic animal catfish. Detailed implementation manners

[0018] Here, in order to more clearly explain the overall concept of the present application, the overall solution of the present invention will be described in detail below by way of examples; in the following description, a large number of specific details are given to provide a more thorough understanding of the present invention; however, it is obvious to those skilled in the art that the present invention can be implemented without one or more of these details; in other examples, in order to avoid confusion with the present invention, some well-known technical features in the art are not described.

[0019] In this application, the soft capsule machine is the Lijiang HSR-300C model; the shrimp oil is the BREAKER Borui Ke P56 Antarctic krill oil; the Saccharomyces cerevisiae is Yamaide CAS 68876-77-7, 50 billion CFU / g; the Lactobacillus plantarum is Yunqi Biology TR-28 Lactobacillus plantarum, 10 billion CFU / g; the β-glucan is Angel Yeast β-glucan G70; the tea polyphenols CAS 84650-60-2; the stachyose CAS 470-55-3; the sodium butyrate CAS 156-54-7; the water content of Atractylodes macrocephala, Coptis chinensis, Cinnamomum cassia, Citrus reticulata Blanco, Sophora flavescens and Glycyrrhiza uralensis is 5%, purchased from Bozhou Deyili Pharmaceutical Sales Co., Ltd.; the above-mentioned traditional Chinese medicines all meet the standards of the "Good Agricultural Practice for Chinese Crude Drugs" and the "Chinese Pharmacopoeia" of the State Food and Drug Administration.

[0020] Unless otherwise specified, the raw material components in the following examples can all be obtained through commercial channels, and the experimental instruments used are all conventional laboratory experimental instruments, and the performance testing methods are known testing methods in the art. The overall operating space environment is 25°C and the air humidity is 30%.

[0021] The preferred embodiments are as follows: Example 1: Prepare the feed raw materials for promoting the intestinal repair of aquatic animals: nutrient solution with a mass fraction of 76% and glue solution to make up the remaining mass fraction; The nutrient solution is evenly mixed: duck blood with a mass fraction of 47%, vitamin C with a mass fraction of 0.7%, tea polyphenols with a mass fraction of 0.2%, Saccharomyces cerevisiae with a mass fraction of 1%, Lactobacillus plantarum with a mass fraction of 1.2%, β-glucan with a mass fraction of 5%, stachyose with a mass fraction of 3%, sodium butyrate with a mass fraction of 0.1%, plant extract with a mass fraction of 33% and pure water to make up the remaining mass fraction; The glue solution is evenly mixed: glycerol with a mass fraction of 16%, gelatin with a mass fraction of 40%, shrimp oil with a mass fraction of 4% and water to make up the remaining mass fraction; The preparation method of the plant extract is as follows: take Atractylodes macrocephala with a mass fraction of 28%, Coptis chinensis with a mass fraction of 23%, Cinnamomum cassia with a mass fraction of 16%, Citrus reticulata Blanco with a mass fraction of 12%, Sophora flavescens with a mass fraction of 10% and Glycyrrhiza uralensis to make up the remaining mass fraction, crush to 10 mesh, add 85% ethanol 6 times the total mass of the above-mentioned medicinal materials and immerse at 25°C for 40 h, filter to obtain the crude filtered extract; add pure water with a mass of 0.2 times the mass of the crude filtered extract to the crude filtered extract and then perform vacuum distillation at 60°C and a vacuum degree of 0.098 Mpa until the mass is 0.2 times that before evaporation to obtain the plant extract.

[0022] After melting the glue solution by stirring at 60°C and then keeping it warm at 55°C, it is fed into the HSR-300C soft capsule machine. The particle size is set to 8 mm, the content is the nutrient solution, and the filling amount per capsule is 800 mm.

[0023] Example 2 - 11: The difference between Example 2 and Example 1 is only that the mass fraction of Saccharomyces cerevisiae is 0.5%; The difference between Example 3 and Example 1 is only that the mass fraction of Saccharomyces cerevisiae is 1.5%; The difference between Example 4 and Example 1 is only that the mass fraction of Lactobacillus plantarum is 0.5%; The difference between Example 5 and Example 1 is only that the mass fraction of Lactobacillus plantarum is 1.5%; The difference between Example 6 and Example 1 is only that the mass fraction of plant extract is 30%; The difference between Example 7 and Example 1 is only that the mass fraction of plant extract is 35%; The difference between Example 8 and Example 1 is only that the mass fraction of Coptis chinensis is 20%; The difference between Example 9 and Example 1 is only that the mass fraction of Coptis chinensis is 25%; The difference between Example 10 and Example 1 is only that the mass fraction of Sophora flavescens is 8%; The difference between Example 11 and Example 1 is only that the mass fraction of Sophora flavescens is 12%.

[0024] Comparative Example 1 - 26: The difference between Comparative Example 1 and Example 1 is only that the mass fraction of vitamin C is 0.2%; The difference between Comparative Example 2 and Example 1 is only that the mass fraction of vitamin C is 2%; The difference between Comparative Example 3 and Example 1 is only that the mass fraction of tea polyphenols is 0.05%; The difference between Comparative Example 4 and Example 1 is only that the mass fraction of tea polyphenols is 1%; The difference between Comparative Example 5 and Example 1 is only that the mass fraction of Saccharomyces cerevisiae is 3%; The difference between Comparative Example 6 and Example 1 is only that the mass fraction of Lactobacillus plantarum is 3%; The difference between Comparative Example 7 and Example 1 is only that the mass fraction of β - glucan is 2%; The difference between Comparative Example 8 and Example 1 is only that the mass fraction of β - glucan is 10%; The difference between Comparative Example 9 and Example 1 is only that the mass fraction of stachyose is 1%; The difference between Comparative Example 10 and Example 1 is only that the mass fraction of stachyose is 8%; The difference between Comparative Example 11 and Example 1 is only that the mass fraction of the plant extract is 40%; The difference between Comparative Example 12 and Example 1 is only that the mass fraction of the shrimp oil is 1%; The difference between Comparative Example 13 and Example 1 is only that the mass fraction of the shrimp oil is 10%; The difference between Comparative Example 14 and Example 1 is only that in the preparation method of the plant extract, the temperature of vacuum distillation is 45 °C; The difference between Comparative Example 15 and Example 1 is only that in the preparation method of the plant extract, the temperature of vacuum distillation is 75 °C; The difference between Comparative Example 16 and Example 1 is only that vitamin C is replaced with the same mass of tea polyphenols; The difference between Comparative Example 17 and Example 1 is only that tea polyphenols are replaced with the same mass of vitamin C; The difference between Comparative Example 18 and Example 1 is only that β-glucan is replaced with the same mass ratio of stachyose and sodium butyrate as in Example 1; The difference between Comparative Example 19 and Example 1 is only that stachyose is replaced with the same mass ratio of β-glucan and sodium butyrate as in Example 1; The difference between Comparative Example 20 and Example 1 is only that sodium butyrate is replaced with the same mass ratio of β-glucan and stachyose as in Example 1.

[0025] The difference between Comparative Example 21 and Example 1 is only that atractylodes macrocephala is replaced with the same mass ratio of coptis chinensis, cinnamon, tangerine peel, sophora flavescens and licorice as in Example 1; The difference between Comparative Example 22 and Example 1 is only that coptis chinensis is replaced with the same mass ratio of atractylodes macrocephala, cinnamon, tangerine peel, sophora flavescens and licorice as in Example 1; The difference between Comparative Example 23 and Example 1 is only that cinnamon is replaced with the same mass ratio of atractylodes macrocephala, coptis chinensis, tangerine peel, sophora flavescens and licorice as in Example 1; The difference between Comparative Example 24 and Example 1 is only that tangerine peel is replaced with the same mass ratio of atractylodes macrocephala, coptis chinensis, cinnamon, sophora flavescens and licorice as in Example 1; The difference between Comparative Example 25 and Example 1 is only that sophora flavescens is replaced with the same mass ratio of atractylodes macrocephala, coptis chinensis, cinnamon, tangerine peel and licorice as in Example 1; The difference between Comparative Example 26 and Example 1 is only that licorice is replaced with the same mass ratio of atractylodes macrocephala, coptis chinensis, cinnamon, tangerine peel and sophora flavescens as in Example 1.

[0026] Blank group example: The difference between the blank group and Example 1 is only that the feed promoting the intestinal repair of aquatic animals is replaced with the same mass of soybean meal feed.

[0027] Prepare 2m corresponding to the quantities of the above respective examples 3The volumetric culture tank is filled with water to 90% and oxygen is pumped in at 5 mL / s. 10 catfish with poor intestines and a body length of 25 ± 1 cm are placed in each tank. The intestinal test substances are obtained by repeatedly gently pressing the anus and sent for testing of the density of Saccharomyces cerevisiae and Lactobacillus plantarum. The initial densities of Saccharomyces cerevisiae and Lactobacillus plantarum in the intestines of each catfish with poor intestines are less than or equal to 10 CFU / g. The feed of the above example is fed into the corresponding culture tank 3 times a day, in the morning, at noon and in the evening, and the feeding amount is 100 g / tank each time. The environmental temperature is 25 °C. After 7 days of the experiment, the intestinal test substances are obtained by repeatedly gently pressing the anus and sent for testing of the density of Saccharomyces cerevisiae and Lactobacillus plantarum. The test results are shown in Table 1, with the unit of CFU / g, and the integer is taken after averaging each tank.

[0028] Table 1: Test results of the density of Saccharomyces cerevisiae and Lactobacillus plantarum in the intestinal test substances of catfish in each tank corresponding to each example

[0029] As can be seen from the data in Table 1, compared with other examples, the feed for promoting the intestinal repair of aquatic animals in the embodiment of the present application, especially Embodiment 1 of the present application, has higher densities of Saccharomyces cerevisiae and Lactobacillus plantarum in the intestinal test substances of catfish in each tank under the same feeding amount; that is, the feed for promoting the intestinal repair of aquatic animals in the embodiment of the present application, especially Embodiment 1 of the present application, has a better effect of increasing the density of beneficial bacteria and promoting the intestinal repair of catfish, an aquatic animal.

[0030] The above are only the embodiments of the present application and are not used to limit the present application; for those skilled in the art, various changes and modifications can be made to the present application; any modification, equivalent replacement, improvement, etc. made within the spirit and principle of the present application shall be included within the scope of the claims of the present application.

Claims

1. A feed for promoting the intestinal repair of aquatic animals, characterized in that, The feed for promoting the intestinal repair of aquatic animals is: nutrient solution with a mass fraction of 76% and glue solution to make up the remaining mass fraction; The nutrient solution is evenly mixed: duck blood with a mass fraction of 47%, vitamin C with a mass fraction of 0.5 - 1%, tea polyphenols with a mass fraction of 0.1 - 0.3%, Saccharomyces cerevisiae with a mass fraction of 0.5 - 1.5%, Lactobacillus plantarum with a mass fraction of 0.5 - 1.5%, β-glucan with a mass fraction of 4 - 6%, stachyose with a mass fraction of 2 - 4%, sodium butyrate with a mass fraction of 0.1%, plant extract with a mass fraction of 30 - 35% and pure water to make up the remaining mass fraction; The glue solution is evenly mixed: glycerol with a mass fraction of 16%, gelatin with a mass fraction of 40%, fish oil with a mass fraction of 3 - 5% and water to make up the remaining mass fraction; The preparation method of the plant extract is as follows: take atractylodes macrocephala with a mass fraction of 28%, coptis chinensis with a mass fraction of 20 - 25%, cinnamon with a mass fraction of 16%, dried tangerine peel with a mass fraction of 12%, sophora flavescens with a mass fraction of 8 - 12% and licorice to make up the remaining mass fraction, crush to 10 mesh, add 85% ethanol with a mass 6 times that of the total mass of the above medicinal materials and immerse at 25°C for 40 h, filter to obtain a crude filtered extract; add pure water with a mass 0.2 times that of the crude filtered extract to the crude filtered extract and then perform vacuum distillation at 60°C and a vacuum degree of 0.098 Mpa until the mass is 0.2 times that before evaporation to obtain the plant extract.

2. The feed for promoting the intestinal repair of aquatic animals according to claim 1, wherein The mass fraction of the vitamin C is 0.7%.

3. The feed for promoting intestinal repair of aquatic animals according to claim 1, characterized in that, The mass fraction of the tea polyphenols is 0.2%.

4. The feed for promoting intestinal repair of aquatic animals according to claim 1, characterized in that, The mass fraction of the Saccharomyces cerevisiae is 1%.

5. The feed for promoting the intestinal repair of aquatic animals according to claim 1, wherein The mass fraction of the Lactobacillus plantarum is 1.2%.

6. The feed for promoting intestinal repair of aquatic animals according to claim 1, characterized in that, The mass fraction of the β-glucan is 5%.

7. The feed for promoting the intestinal repair of aquatic animals according to claim 1, wherein The mass fraction of the stachyose is 3%.

8. The feed for promoting intestinal repair of aquatic animals according to claim 1, wherein The mass fraction of the plant extract is 33%.

9. The feed for promoting the intestinal repair of aquatic animals according to claim 1, wherein The mass fraction of the fish oil is 4%.

10. The feed for promoting intestinal repair of aquatic animals according to claim 1, wherein, The mass fraction of the coptis chinensis is 23%.

11. The feed for promoting intestinal repair of aquatic animals according to claim 1, wherein The mass fraction of the sophora flavescens is 10%.

12. A method for preparing a feed for promoting intestinal repair of aquatic animals as described in any one of claims 1 to 11, characterized in that, The preparation method of the feed for promoting the intestinal repair of aquatic animals is: stir and gelatinize the glue solution at 60°C, keep it warm at 55°C and connect it to an HSR-300C soft capsule machine, set the particle size to 8 mm, the content is the nutrient solution, and the filling amount per capsule is 800 mm.

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