Fat-reducing composition as well as preparation method and application thereof

Abstract

The invention discloses a fat-reducing composition as well as a preparation method and application thereof. The fat-reducing composition comprises a hippophae rhamnoides leaf extract and a first mixed component and / or a second mixed component, wherein the first mixed component comprises an ampelopsis grossedentata extract and a fructus momordicae extract, the second mixed component comprises first coffee freeze-dried powder and second coffee freeze-dried powder, and the second coffee freeze-dried powder contains chlorogenic acid. The preparation method of the fat-reducing composition comprises the step of mixing all the components. All the components of the fat-reducing composition provided by the invention have a better fat-reducing effect through synergistic interaction, all the components belong to safe and side-effect-free components, dependence is not generated, and fat can be reduced more effectively.

Description

Technical Field

[0001] The present invention relates to the technical field of health foods, and particularly relates to a fat-reducing composition, a preparation method thereof, and an application thereof. Background Art

[0002] Obesity can generally be divided into simple obesity and secondary obesity. Simple obesity refers to a condition in which the intake of calories in the body is greater than the consumption, resulting in excessive accumulation of fat in the body and an abnormal body weight. The most common type of obesity among various types is simple obesity, accounting for more than 95% of obesity.

[0003] The body mass index (BMI), which is often calculated from height and weight, is used as an indicator to reflect overall obesity: BMI = weight (kg) / height 2 (m 2 ). The "Guidelines for the Prevention and Control of Overweight and Obesity in Chinese Adults" defines that 18.5 ≤ BMI < 24 is normal; 24 ≤ BMI < 28 is overweight; ≥ 28 is obese.

[0004] According to the latest report of "Diabetes, Obesity and Metabolism", in 2023, the proportion of overweight people in China was 34.8%, and the proportion of obese people was 14.1%.

[0005] Obesity is both an independent disease and a risk factor for type 2 diabetes, cardiovascular disease, hypertension, stroke, and various cancers, and is one of the top ten risk factors leading to the disease burden.

[0006] The existing weight-loss products on the market are mainly drugs and health products. Drugs have a fat-reducing effect, but they have strong dependence, large side effects, and the weight is easy to rebound after stopping the drug. The fat-reducing effect of health products is weak. Some products reduce weight by causing diarrhea, which harms physical health. Health products containing anthraquinone components have strong dependence and side effects.

[0007] Obese people are in urgent need of safe, effective, and non-dependent fat-reducing products. Summary of the Invention

[0008] The technical problem to be solved by the present invention is to provide a fat-reducing composition, a preparation method thereof, and an application thereof. Among them, the fat-reducing composition is safe, effective, and non-dependent, suitable for use in the preparation of fat-reducing products, and the preparation method of the fat-reducing composition is simple and convenient for industrial application.

[0009] To solve the above technical problem, the first aspect of the present invention is to provide a fat-reducing composition, comprising seabuckthorn leaf extract; and a first mixed component and / or a second mixed component; Among them, the first mixed component includes Ampelopsis grossedentata extract and Momordica grosvenori extract, the second mixed component includes the first instant coffee powder and the second instant coffee powder, and the second instant coffee powder contains chlorogenic acid.

[0010] In the fat-reducing composition provided by the present invention, the seabuckthorn leaves are the leaves of seabuckthorn. Seabuckthorn (Hippophae rhamnoides L.) belongs to the genus Hippophae of the Elaeagnaceae family. The seabuckthorn leaf extract mainly includes polyphenolic compounds, which can play a role in reducing abdominal fat; the Ampelopsis grossedentata extract mainly includes flavonoid compounds such as dihydromyricetin, which can play a role in improving glucose and lipid metabolism and improving insulin resistance; the Momordica grosvenori extract contains mogroside V, which can play a role in weight loss and lipid reduction and has extremely high safety; chlorogenic acid in the second instant coffee powder can play a role in reducing lipid synthesis and down-regulating the expression of inflammatory factors, and also play a role in lowering blood sugar; the first instant coffee powder mainly plays a role as a carrier. When the seabuckthorn leaf extract is mixed with the first mixed component (a mixture of Ampelopsis grossedentata extract and Momordica grosvenori extract), or when the seabuckthorn leaf extract is mixed with the second mixed component (the first instant coffee powder and the second instant coffee powder), or when the seabuckthorn leaf extract is mixed with the first mixed component and the second mixed component to form a fat-reducing composition, there is a synergistic fat-reducing effect among the components. Moreover, each component in the present invention belongs to components that are safe and have no side effects and do not produce dependence.

[0011] It should be noted that the caffeine content in the first instant coffee powder and the second instant coffee powder in the present invention can be the same or different. Preferably, the caffeine content in the first instant coffee powder is higher than that in the second instant coffee powder to provide the aroma and taste of coffee. For example, the caffeine content in the first instant coffee powder can be 3.97 wt%, and the caffeine content in the second instant coffee powder can be 2.07 wt%. In addition, it should also be noted that although there is research indicating that caffeine can, to a certain extent, increase fat metabolism and reduce the content of free fatty acids and glycerol in the blood, its dosage needs to reach 425 mg / d and be combined with an energy-restricted diet to have an effect. However, after a large amount of caffeine is ingested by the human body, it may cause side effects such as palpitations and headaches in caffeine-intolerant people. When the fat-reducing composition provided by the present invention is used in the preparation of a fat-reducing product, the caffeine content in each portion of the fat-reducing product (one-day dosage) is less than 80 mg to avoid adverse effects on caffeine-intolerant people.

[0012] Specifically, the fat-reducing composition includes, by mass: 2 to 12 parts by mass, or 2 parts by mass, or 8 parts by mass, or 12 parts by mass of seabuckthorn leaf extract, preferably 8 parts by mass. Among them, the content of polyphenolic compounds is not less than 20 wt% or not less than 25 wt%, preferably not less than 25 wt%. 1 - 13 parts by mass, or 1 part by mass, or 4 parts by mass, or 13 parts by mass of Ampelopsis grossedentata extract, preferably 4 parts by mass, and its dihydromyricetin content is not less than 30 wt% or not less than 40 wt%, preferably not less than 40 wt%; 1 - 4 parts by mass, or 1 part by mass, or 3 parts by mass, or 4 parts by mass of Siraitia grosvenorii extract, preferably 4 parts by mass, and its mogroside V content is not less than 1 wt% or not less than 1.5 wt%, preferably not less than 1.5 wt%.

[0013] Specifically, the fat - reducing composition includes, by mass: 2 - 12 parts by mass, or 2 parts by mass, or 8 parts by mass, or 12 parts by mass of Hippohgae rhamnoides L. leaf extract, preferably 8 parts by mass, and the content of polyphenolic compounds is not less than 20 wt% or not less than 25 wt%, preferably not less than 25 wt%; 9 - 33 parts by mass, or 9 parts by mass, or 26 parts by mass, or 33 parts by mass of the first instant coffee powder, preferably 26 parts by mass; 11 - 35 parts by mass, or 11 parts by mass, or 18 parts by mass, or 35 parts by mass of the second instant coffee powder, preferably 18 parts by mass, and the content of chlorogenic acid is 10 wt% - 15 wt% or 10 wt% - 12 wt% or 12 wt% - 15 wt% or 12 wt%, preferably 12 wt%.

[0014] Specifically, the fat - reducing composition includes, by mass: 2 - 12 parts by mass, or 2 parts by mass, or 8 parts by mass, or 12 parts by mass of Hippohgae rhamnoides L. leaf extract, preferably 8 parts by mass, and the content of polyphenolic compounds is not less than 20 wt% or not less than 25 wt%, preferably not less than 25 wt%; 1 - 13 parts by mass, or 1 part by mass, or 4 parts by mass, or 13 parts by mass of Ampelopsis grossedentata extract, preferably 4 parts by mass, and its dihydromyricetin content is not less than 30 wt% or not less than 40 wt%, preferably not less than 40 wt%; 1 - 4 parts by mass, or 1 part by mass, or 3 parts by mass, or 4 parts by mass of Siraitia grosvenorii extract, preferably 4 parts by mass, and its mogroside V content is not less than 1 wt% or not less than 1.5 wt%, preferably not less than 1.5 wt%; 9 - 33 parts by mass, or 9 parts by mass, or 26 parts by mass, or 33 parts by mass of the first instant coffee powder, preferably 26 parts by mass; 11 - 35 parts by mass, or 11 parts by mass, or 18 parts by mass, or 35 parts by mass of the second instant coffee powder, preferably 18 parts by mass, and the content of chlorogenic acid is 10 wt% - 15 wt% or 10 wt% - 12 wt% or 12 wt% - 15 wt% or 12 wt%, preferably 12 wt%.

[0015] In the present invention, when the components in the fat-reducing composition are in preferred parts by mass, the synergistic effect among the components is the most obvious, which can significantly reduce body weight, BMI, waist circumference, hip circumference and body fat percentage (P < 0.05), subcutaneous fat thickness (P < 0.05), the SF-36 score increases significantly by 2.53% (P < 0.05), and the ZWQOL score decreases significantly by 17.86% (P < 0.01).

[0016] Specifically, the seabuckthorn leaf extract is obtained by the following method: S1. Pretreatment of seabuckthorn leaves: After crushing the seabuckthorn leaves, sieve them for later use; S2. Preparation of extraction solution: Add pure water to the seabuckthorn leaves pretreated in step S1, the added mass of pure water is 10 - 20 times the mass of the seabuckthorn leaves, then heat the temperature of the mixed system to 70°C - 95°C, stir for 30 - 100 min, and filter the liquid through a sieve with 50 - 200 meshes to obtain the extraction solution; S3. Post-treatment of extraction solution: Centrifuge the extraction solution obtained in step S2 at a rotation speed of 2000 - 5000 rpm for 2 - 20 min, take the clarified liquid, concentrate the clarified liquid at 20 - 80°C and a pressure of 40 - 70 mbar until the Brix reaches 9 - 12%, and then carry out UHT sterilization treatment to obtain the concentrated extraction solution; S4. Spray drying: Spray dry the concentrated extraction solution obtained in step S3 under the conditions of an inlet temperature of 155 - 185°C and an outlet temperature of 80 - 90°C to obtain the powder of the seabuckthorn leaf extract.

[0017] In the present invention, in step S1, the mesh number of the sieved seabuckthorn leaves after crushing is, for example, 30 meshes or 40 meshes, which is convenient for extraction and is beneficial to reducing the dust loss and the risk of equipment blockage during the processing; in addition, the drying of the concentrated extraction solution is not limited to the spray drying method. The preferred spray drying purpose of the present invention is to directly powder the seabuckthorn leaf extract, with high production efficiency and good particle uniformity, which is convenient for subsequent applications.

[0018] To solve the above technical problems, the second aspect of the present invention is to provide an application of the aforementioned fat-reducing composition in the preparation of fat-reducing products. It can be understood that because the fat-reducing compositions provided by the present invention have a synergistic effect, they have significant effects such as reducing body weight, BMI, waist circumference, hip circumference and body fat percentage (P < 0.05), subcutaneous fat thickness (P < 0.05), etc., and the SF-36 score increases significantly by 2.53% (P < 0.05), and the ZWQOL score decreases significantly by 17.86% (P < 0.01). Therefore, the fat-reducing products prepared after its application in the preparation of fat-reducing products at least have all the beneficial effects of the fat-reducing composition. It should be noted that the fat-reducing products in the present invention include oral liquids, granules, powders, capsules or tablets.

[0019] To solve the above technical problems, the third aspect of the present invention is to provide a preparation method of the aforementioned fat-reducing composition, which is to uniformly mix the components of the fat-reducing composition to obtain the fat-reducing composition. For example, first powder the Ampelopsis grossedentata extract and Momordica grosvenori extract and mix them in the required proportion to obtain the first mixed component, then powder the first instant coffee powder and the second instant coffee powder and mix them in the required proportion to obtain the second mixed component, and finally powder the Hippohgae rhamnoides L. leaf extract as needed and mix it with the first mixed component and / or the second mixed component in the required proportion; in the present invention, the mixing can be carried out by stirring in a mixing tank, for example.

[0020] In the present invention, the method for powdering the Hippohgae rhamnoides L. leaf extract includes extracting the Hippohgae rhamnoides L. leaf extract from Hippohgae rhamnoides L. leaves using pure water as a solvent, and then obtaining the powdered Hippohgae rhamnoides L. leaf extract by spray drying; specifically, for example, first crush the Hippohgae rhamnoides L. leaves, pass through a 30-mesh sieve (or a 40-mesh sieve), then add pure water with a mass 10-20 times that of the Hippohgae rhamnoides L. leaf powder, heat to 70-95 °C and stir for 30-100 min, filter the liquid through a 50-200-mesh sieve, then centrifuge at 2000-5000 rpm for 2-20 min, take the clarified liquid, stop concentrating the clarified liquid when it is concentrated to Brix 9-12% at 20-80 °C and 40-70 mbar pressure, and spray dry the concentrated extract at an inlet temperature of 155-185 °C and an outlet temperature of 80-90 °C to obtain the powdered Hippohgae rhamnoides L. leaf extract.

[0021] In the present invention, the first instant coffee powder and the powdered second instant coffee powder can be obtained by the following method, for example: purchase the commercially available first instant coffee powder and the second instant coffee powder, mix them in the required proportion and then crush them, and pass through a 30-mesh sieve (or a 40-mesh sieve) to reduce the original particle size for easy mixing. Preferably, the Hippohgae rhamnoides L. leaf extract, Ampelopsis grossedentata extract, Momordica grosvenori extract, the first instant coffee powder and the second instant coffee powder are all powder materials.

[0022] In the present invention, both the Ampelopsis grossedentata extract and the powdered Momordica grosvenori extract are commercially available or can be used after passing through a 30-mesh sieve (or a 40-mesh sieve).

[0023] The fat-reducing composition of the present invention aims at uniform mixing. When stirring, the order of adding each material does not limit the present invention. The preparation method of the fat-reducing composition of the present invention is simple to operate and is convenient for large-scale and industrial applications. Detailed Embodiments

[0024] The technical solutions in the present invention will be clearly and completely described below. Obviously, the described embodiments are some of the embodiments of the present invention, rather than all of the embodiments. All other embodiments obtained by those of ordinary skill in the art based on the embodiments in the present invention without creative efforts shall fall within the protection scope of the present invention.

[0025] Example 1 Preparation of Fat-reducing Composition 1. Material Preparation 1) Preparation of Powdered Sea Buckthorn Leaf Extract S1. Pretreatment of Sea Buckthorn Leaves: After crushing the sea buckthorn leaves, sieve them through a 30-mesh sieve for standby; S2. Preparation of Extract Solution: Add 14 times the mass of pure water to the crushed sea buckthorn leaves pretreated in step S1, heat to 95 °C, stir for 60 min, filter the liquid through an 80-mesh sieve to obtain the extract solution; S3. Post-treatment of Extract Solution: Centrifuge the extract solution obtained in step S2 at a speed of 4000 rpm for 5 min, take the clarified liquid, concentrate the clarified liquid at 55 °C and a pressure of 50 mbar until the Brix reaches 10%, then stop and perform UHT sterilization treatment to obtain the concentrated extract solution; S4. Spray Drying: Take the concentrated and sterilized extract solution obtained in step S3, add it to the spray drying equipment, set the inlet temperature at 175 °C and the outlet temperature at 80 °C, and obtain the powdered sea buckthorn leaf extract through spray drying.

[0026] 2) Powdered Ampelopsis grossedentata extract (dihydromyricetin content is 40 wt%) and powdered Momordica grosvenori extract (mogroside V content is 1.5 wt%) are both purchased from Huayuanlv Bio-Tech Co., Ltd. and can be used directly.

[0027] 3) Preparation of Mixture of Powdered First Coffee Freeze-dried Powder and Second Coffee Freeze-dried Powder Take the first coffee freeze-dried powder and the second coffee freeze-dried powder commissioned by Shanghai Touchuang Network Technology Co., Ltd. and already on the market. Among them, the chlorogenic acid content in the second coffee freeze-dried powder is 12 wt%. After mixing the first coffee freeze-dried powder and the second coffee freeze-dried powder in proportion, add them to a pulverizer for pulverization, and sieve through a 40-mesh sieve to obtain the mixture of powdered first coffee freeze-dried powder and second coffee freeze-dried powder.

[0028] 2. Preparation of Fat-reducing Composition 1) Put the prepared sea buckthorn leaf extract, Ampelopsis grossedentata extract and Momordica grosvenori extract into the mixing tank in proportion and stir for 30 min.

[0029] 2) Put the mixture of powdered first coffee freeze-dried powder and second coffee freeze-dried powder into the mixing tank in step 1), continue to stir for 30 min. After metal detection, discharge the material for standby.

[0030] The addition amounts of each component of the sample prepared in this example are shown in Table 1.

[0031] Table 1

[0032] Example 2 Efficacy Evaluation of Fat-reducing Composition on Mice Experimental animals: 6-week-old male C57BL / 6J mice; Experimental method: The mice were fed a high-fat diet (D12492) containing 60 kcal% fat. After four weeks of high-fat modeling, mice with significant weight gain were selected and randomly divided into 13 groups, with 8 mice in each group, which were used for the model group, experimental groups 1-5, and control groups 1-7. During this period, another 8 mice were fed a maintenance diet (without high-fat modeling) as the blank group. Then, the 14 groups of mice were normally fed for 8 weeks. Among them, the mice in experimental groups 3-5 were respectively given the fat-reducing compositions in experimental groups 3-5 of Example 1 at a dose of 455 mg / kg per day, the mice in experimental group 1 were given the mixture in experimental group 1 of Example 1 at a dose of 394 mg / kg, the mice in experimental group 2 were given the mixture in experimental group 2 of Example 1 at a dose of 121 mg / kg, the mice in control group 1 were given the seabuckthorn leaf extract in control group 1 of Example 1 at a dose of 61 mg / kg per day, the mice in control group 2 were given the tengcha extract in control group 2 of Example 1 at a dose of 30 mg / kg per day, the mice in control group 3 were given the momordica grosvenori extract in control group 3 of Example 1 at a dose of 30 mg / kg per day, the mice in control group 4 were given the mixture in control group 4 of Example 1 at a dose of 334 mg / kg per day, the mice in control group 5 were given the mixture in control group 5 of Example 1 at a dose of 364 mg / kg, the mice in control group 6 were given the mixture in control group 6 of Example 1 at a dose of 364 mg / kg, and the mice in control group 7 were given the mixture in control group 7 of Example 1 at a dose of 61 mg / kg. The model group and the blank group were given an equal amount of the corresponding solvent (in this example, the solvent in the experimental group and the control group is physiological saline. During feeding, the required amount of solid powder is dissolved in physiological saline and then supplied to the mice for drinking). During the feeding period, the body weight was measured weekly. After the feeding ended, the liver weight, abdominal fat weight, and subcutaneous fat weight were measured, and fasting blood glucose and insulin were detected. The HOMA-IR index was calculated, and the activities of fatty acid synthase (FAS) and carnitine palmitoyltransferase (CPT) in the liver were detected.

[0033] Among them, the HOMA-IR index = fasting blood glucose level (mmol / L) × fasting insulin level (mIU / L) / 22.5; the insulin unit conversion is: 1 ng / ml × 21.2 = μIU / ml (mIU / L); In this example, the synergistic effect q was calculated according to the King's formula:

[0034] In the formula, EA and EB are the effects of A and B acting alone, E(A + B) is the effect when the two are used together, and when the q value is between 0.85 and 1.15, it is simple addition; when the q value is between 1.15 and 20, it is enhancement; when the q value is greater than 20, it is significant enhancement; when the q value is between 0.56 and 0.85, it is antagonism; when the q value is less than 0.55, it is significant antagonism.

[0035] In this embodiment, for example, the use of the mixture of seabuckthorn leaf extract, tengcha extract and momordica grosvenori extract is defined as the individual action of EA, the use of the mixture of the first instant coffee powder and the second instant coffee powder is defined as the individual action of EB, and the use of the fat-reducing composition formed by mixing the extract, tengcha extract, momordica grosvenori extract, the first instant coffee powder and the second instant coffee powder is defined as the combined use of E(A+B).

[0036] Test results 1) The test results of the body weight changes of each group of mice are shown in Tables 2-1 and 2-2, and the weight loss synergistic effect among groups is shown in Table 3: Table 2-1

[0037] Table 2-2

[0038] In Tables 2-1 and 2-2, those with # indicate P < 0.05 compared with the blank group, those with ## indicate P < 0.01 compared with the blank group, those with * indicate P < 0.05 compared with the model group, and those with ** indicate P < 0.01 compared with the model group.

[0039] Table 3

[0040] In Table 3, those with * indicate P < 0.05 compared with the model group, and those with ** indicate P < 0.01 compared with the model group.

[0041] From Tables 2-1 and 2-2, it can be seen that there is no significant difference in the initial body weights of each group of mice (P < 0.05). Starting from the first week, compared with the model group, the body weights of the mice in experimental groups 3 to 5 decreased significantly (P < 0.05). Among them, the body weight of the mice in experimental group 5 decreased the most. At the eighth week, the body weight of the mice in experimental group 5 decreased by 21.5%. However, in control group 1, experimental groups 1 and 2, the weight loss effect only started to appear from the third week. And there was no weight loss effect in other control groups.

[0042] Experimental group 5 is equivalent to the combination of control group 4 and experimental group 2, or experimental group 1 and control group 7. The q values calculated by the King's formula are 1.36 and 1.40 respectively, belonging to the level of enhanced effect. See Table 3 for details. Experimental group 1 is composed of control group 1 and 4, and the q value is 1.11. The combination of seabuckthorn leaf extract powder and coffee mixed powder only has a simple additive weight loss effect. Control group 5 is composed of control group 2 and 4, and control group 6 is composed of control group 3 and 4. The above-mentioned ampelopsis grossedentata extract powder and momordica grosvenori extract powder are used alone in combination with coffee mixed powder, and there is no improvement in efficacy. Control group 7 is composed of control group 2 and 3, and there is no improvement in the combination of ampelopsis grossedentata and momordica grosvenori either. Experimental group 2 is composed of control group 1 and 7, and the q value is 1.16. The combination of seabuckthorn leaf extract, ampelopsis grossedentata extract and momordica grosvenori extract has a synergistic effect on inhibiting weight gain.

[0043] From the measurement of the body weights of each group of mice, it can also be seen that the combination of seabuckthorn leaf extract, ampelopsis grossedentata extract and momordica grosvenori extract has a synergistic effect on reducing body weight, or the synergistic effect of the combination of seabuckthorn leaf extract, ampelopsis grossedentata extract, momordica grosvenori extract, the first instant coffee powder and the second instant coffee powder on reducing body weight is further improved, which is better than the mixture of seabuckthorn leaf extract, ampelopsis grossedentata extract and momordica grosvenori extract used alone, or better than the mixture of the first instant coffee powder and the second instant coffee powder used alone, or better than seabuckthorn leaf extract used alone, or better than ampelopsis grossedentata extract used alone, or better than momordica grosvenori extract used alone.

[0044] 2) The measurement results of the liver weight, abdominal fat weight and subcutaneous fat weight of each group of mice are shown in Table 4: Table 4

[0045] In Table 4, those with # indicate P < 0.05 compared with the blank group, those with ## indicate P < 0.01 compared with the blank group, those with * indicate P < 0.05 compared with the model group, and those with ** indicate P < 0.01 compared with the model group.

[0046] It can be seen from Table 4 that compared with the blank group, the abdominal fat weight and subcutaneous fat weight of the mice in the model group increased significantly (P < 0.01). Compared with the model group, experimental groups 1-5 were all able to significantly reduce the liver weight (P < 0.05). Experimental groups 1 to 5 and control group 1 were able to reduce the abdominal fat weight and subcutaneous fat weight (P < 0.05). Experimental group 5 had a better effect on reducing abdominal fat and subcutaneous fat. The other control groups had no significant effect on reducing the liver, abdominal fat and subcutaneous fat weights (P > 0.05).

[0047] Through the calculation of the synergy coefficient, the calculation results of abdominal fat and subcutaneous fat are shown in Tables 5 and 6 respectively: Table 5

[0048] Table 6

[0049] Tables 5 and 6. Values with # indicate P < 0.05 compared with the blank group, values with ## indicate P < 0.01 compared with the blank group, values with * indicate P < 0.05 compared with the model group, and values with ** indicate P < 0.01 compared with the model group.

[0050] As can be seen from Table 5, for reducing abdominal fat, Experimental Group 5 is equivalent to the combination of Control Group 4 and Experimental Group 2 or the combination of Experimental Group 1 and Control Group 7. The q values calculated by the King's formula are 1.44 and 1.49 respectively, belonging to the enhanced effect level, indicating that the above combinations can synergistically inhibit the increase of abdominal fat. Experimental Group 1 is composed of Control Group 1 and 4, and the q value is 1.10. The combination of seabuckthorn leaf extract powder and coffee mixed powder only shows a simple additive weight loss effect. Control Group 5 is composed of Control Group 2 and 4, and Control Group 6 is composed of Control Group 3 and 4. When the above-mentioned tengcha extract powder or luohanguo extract powder is combined with coffee mixed powder respectively, there is no improvement in efficacy. Control Group 7 is composed of Control Group 2 and 3, and the q value is 1.22. The combination of tengcha and luohanguo can synergistically inhibit abdominal fat. Experimental Group 2 is composed of Control Group 1 and 7, and the q value is 1.17. The combination of seabuckthorn leaf extract, tengcha extract and luohanguo extract has a synergistic effect on inhibiting the increase of abdominal fat.

[0051] As can be seen from Table 6, for reducing subcutaneous fat, Experimental Group 5 is equivalent to the combination of Control Group 4 and Experimental Group 2 or the combination of Experimental Group 1 and Control Group 7. The q values calculated by the King's formula are 1.78 and 1.80 respectively, belonging to the enhanced effect level, indicating that the above combinations can synergistically inhibit the increase of subcutaneous fat. Experimental Group 1 is composed of Control Group 1 and 4, and the q value is 1.28. The combination of seabuckthorn leaf extract powder and coffee mixed powder can also synergistically enhance the effect of inhibiting subcutaneous fat. Control Group 5 is composed of Control Group 2 and 4, and Control Group 6 is composed of Control Group 3 and 4. When the above-mentioned tengcha extract powder and luohanguo extract powder are combined with coffee mixed powder respectively, there is no improvement in efficacy. Control Group 7 is composed of Control Group 2 and 3, and the q value is 1.01. The combination of tengcha and luohanguo mixed powder only shows a simple additive effect. Experimental Group 2 is composed of Control Group 1 and 7, and the q value is 1.31. The combination of seabuckthorn leaf extract, tengcha extract and luohanguo extract has a synergistic effect on inhibiting the increase of subcutaneous fat.

[0052] As can be seen from Tables 5 to 6, the combined use of the seabuckthorn leaf extract, tengcha extract, and momordica grosvenori extract provided by the present invention has a better effect than the individual use of the seabuckthorn leaf extract, tengcha extract, and momordica grosvenori extract. The combined use of the seabuckthorn leaf extract, tengcha extract, momordica grosvenori extract, the first coffee freeze-dried powder, and the second coffee freeze-dried powder has a better effect than the combined use of the seabuckthorn leaf extract, tengcha extract, and momordica grosvenori extract, and also better than the combined use of the first coffee freeze-dried powder and the second coffee freeze-dried powder. Moreover, the fat-reducing composition of the present invention has a synergistically enhanced effect, and the effect of experimental group 5 is better.

[0053] In addition, it can also be seen that the combination of the seabuckthorn leaf extract powder and the coffee mixed powder can also synergistically increase the subcutaneous fat inhibition effect. No such synergistic effect was observed in the combination of the tengcha extract, momordica grosvenori extract, and coffee powder.

[0054] 3) The effects on the fasting blood glucose, insulin, and HOMA-IR of mice in each group (`X±S) are shown in Table 7: Table 7

[0055] In Table 7, those with # indicate P < 0.05 compared with the blank group, those with ## indicate P < 0.01 compared with the blank group, those with * indicate P < 0.05 compared with the model group, and those with ** indicate P < 0.01 compared with the model group.

[0056] As can be seen from Table 7, compared with the blank group, the fasting blood glucose FBG, insulin, and HOMA-IR index of the model group increased significantly (P < 0.01). Compared with the model group, experimental groups 2, 3, 5, and control group 1 were able to significantly reduce the fasting blood glucose FBG (P < 0.05). Experimental groups 2 to 5, and control group 1 were able to significantly reduce the insulin level and HOMA-IR index (P < 0.05). Experimental group 5 had the best effect in reducing fasting blood glucose. The results showed that control group 4 had no significant improvement effect on the fasting blood glucose, insulin, and HOMA-IR of mice. After consuming the fat-reducing composition provided by the present invention, a significant effect of reducing blood glucose and alleviating insulin resistance was shown.

[0057] 4) The results of the effects on the activities of fatty acid synthase FAS and carnitine palmitoyltransferase (CPT) in the livers of mice in each group (`X±S) are shown in Table 8: Table 8

[0058] In Table 8, those with # indicate P < 0.05 compared with the blank group, those with ## indicate P < 0.01 compared with the blank group, those with * indicate P < 0.05 compared with the model group, and those with ** indicate P < 0.01 compared with the model group.

[0059] As can be seen from Table 8, compared with the blank group, the CPT-1 activity in the model group was significantly decreased (P < 0.01), suggesting that a high-fat diet impairs lipid transport and metabolism. Compared with the model group, in Experimental Groups 1 to 5 and Comparative Example 1, the CPT-1 activity was significantly increased (P < 0.05), suggesting that the seabuckthorn leaf extract, the mixture formed by the seabuckthorn leaf extract and coffee powder, the mixture formed by the seabuckthorn leaf extract, the ampelopsis grossedentata extract and the momordica grosvenori extract, and the fat-reducing composition of the present invention can all increase the entry of acyl-CoA into mitochondria and promote fatty acid β-oxidation.

[0060] It can also be seen from Table 8 that compared with the blank group, the FAS activity in the model group was significantly increased (P < 0.01), suggesting that a high-fat diet induces an increase in the activity of the lipid synthase FAS in the liver, promoting fat synthesis. Compared with the model group, Experimental Group 5 could significantly decrease the FAS activity (P < 0.05). Experimental Groups 1 - 4 and Comparative Groups 1 to 7 had no significant effect. This suggests that the fat-reducing composition in Experimental Group 5 can reduce fat synthesis by reducing FAS activity.

[0061] It can be seen from this example that the fat-reducing composition provided by the present invention can significantly reduce the accumulation of fat in the liver, subcutaneous and abdominal areas, reduce the fat volume, and can break down fat by reducing fat synthesis (FAS) and promoting fatty acid transport to undergo β-oxidation (CPT-1), alleviating insulin resistance, showing excellent fat-reducing and weight-reducing effects.

[0062] Example 3: Functional evaluation of the fat-reducing composition of Experimental Group 5 in a human feeding trial Subject baseline information Table 9 shows 30 participants (7 males and 23 females), with an average age of 38.73 ± 7.21 years, and the average BMI of the participants was 23.54 ± 3.67 kg / m 2 . The subjects were overweight or not overweight but dissatisfied with their body weight and shape.

[0063] Table 9

[0064] 2) Effects of the fat-reducing composition of Experimental Group 5 on the body weight and BMI of the subjects Table 10 shows the effects of the fat-reducing composition of Experimental Group 5 on the body weight and BMI of the subjects: Table 10

[0065] In Table 10, those with # indicate P < 0.05 compared with before taking; those with ## indicate P < 0.01 compared with before taking; those with * indicate P < 0.05 compared with the first month of taking; those with ** indicate P < 0.01 compared with the first month of taking. As can be seen from Table 10, compared with the weight before taking, after one month and two months of taking the fat-reducing composition of Experimental Group 5, both the weight and BMI value decreased significantly (p < 0.05). The change value was obtained by subtracting the value after taking from the value before taking. Compared with the change value in the first month, the change in the second month increased significantly. The weight decreased significantly by (1.11 ± 1.33) kg (p < 0.05) in the second month, a decrease of 1.71%, and the BMI value decreased significantly by (0.34 ± 0.47) kg / m 2 (p < 0.05), a decrease of 1.45%.

[0066] 3) Effects of the fat-reducing composition of Experimental Group 5 on the waist circumference, hip circumference and waist-hip ratio (WHR) of the subjects Table 11 shows the effects of the fat-reducing composition of Experimental Group 5 on the waist circumference, hip circumference and waist-hip ratio (WHR) of the subjects: Table 11

[0067] In Table 11, those with # indicate P < 0.05 compared with before taking; those with ## indicate P < 0.01 compared with before taking; those with * indicate P < 0.05 compared with the first month of taking; those with ** indicate P < 0.01 compared with the first month of taking.

[0068] As can be seen from Table 11, compared with the circumference before taking, after one month and two months of taking the fat-reducing composition of Experimental Group 5, the waist circumference, hip circumference and WHR values all decreased significantly (p < 0.05). The change value was obtained by subtracting the value after taking from the value before taking. Compared with the value reduced in the first month, more was reduced in the second month. The above data had no statistical difference. The waist circumference decreased by 2.16% in the first month and 2.35% in the second month. Maybe because a relatively large amount was reduced in the first month, so compared with the change value in the first month, the change in the second month had no significant difference.

[0069] 4) Effects of the fat-reducing composition of Experimental Group 5 on the body fat percentage of the subjects Table 12 shows the effects of the fat-reducing composition of Experimental Group 5 on the body fat percentage of the subjects Table 12

[0070] In Table 12, # indicates P < 0.05 compared with before taking the medicine; ## indicates P < 0.01 compared with before taking the medicine; * indicates P < 0.05 compared with the first month of taking the medicine; ** indicates P < 0.01 compared with the first month of taking the medicine.

[0071] As can be seen from Table 12, compared with the weight before taking the fat-reducing composition, the body fat percentage of experimental group 5 was significantly reduced after taking the fat-reducing composition for one and two months (p<0.05), but there was no statistical difference. The body fat percentage decreased by 3.71% in the first month. Compared with the change value in the first month, the body fat percentage in the second month decreased significantly by (2.12±2.27)% (p<0.05), a decrease of 6.34%, and the body fat percentage decreased faster in the second month.

[0072] 5) Effect of the fat-reducing composition of experimental group 5 on the thickness of subcutaneous fat in subjects Table 13 shows the effect of the fat-reducing composition of experimental group 5 on the subcutaneous fat thickness of subjects at four points A, B, C, and D (point A is the midpoint of the outer side of the arm at the lower edge of the right deltoid muscle, point B is the lower angle of the right scapula, point C is 3 cm beside the right umbilicus, and point D is the right anterior superior iliac spine): Table 13

[0073] In Table 13, # indicates P < 0.05 compared with before taking the medicine; ## indicates P < 0.01 compared with before taking the medicine; * indicates P < 0.05 compared with the first month of taking the medicine; ** indicates P < 0.01 compared with the first month of taking the medicine.

[0074] It can be seen from Table 13 that the thickness of subcutaneous fat at points A, B, C, and D was significantly reduced compared to before taking the fat-reducing composition of experimental group 5 for one month (P < 0.05). After taking the fat-reducing composition of experimental group 5 for two months, the thickness of subcutaneous fat at points A, B, C, and D was significantly reduced (P < 0.05), of which point D was reduced by 16.57% (P < 0.05). Example 3 can reduce the thickness of subcutaneous fat at various parts of the body. Point D was reduced by 16.57% (P < 0.05), which shows that Example 3 has a more significant reduction in the thickness of subcutaneous fat at the anterior superior iliac spine at point D.

[0075] Compared with the change value in the first month, the change value of point A at the lower edge of the deltoid muscle of the right arm in the second month was significantly reduced by (2.04±3.15) cm (p<0.05), a decrease of 10.23%. The second month reduced the growth rate of subcutaneous fat thickness.

[0076] 6) Effect of the fat-reducing composition of experimental group 5 on the quality of life of the subjects Table 14 shows the effect of the fat-reducing composition of experimental group 5 on the quality of life of the subjects, and Table 15 shows the SF-36 score results: Table 14

[0077] Table 15

[0078] In Table 14 and Table 15, "#" indicates that P < 0.05 compared with before taking; "##" indicates that P < 0.01 compared with before taking.

[0079] It can be seen from Table 14 that compared with before taking, after taking the fat-reducing composition of Experimental Group 5 for one month, both the ZWQOL score and the SF-36 score showed an improving trend. After taking it for two months, the SF-36 score increased significantly by 2.53% (p < 0.05), and the ZWQOL score decreased significantly by 17.86% (p < 0.01). The results show that after taking it for two months, the formula can improve the quality of life of the subjects and reduce the impact of body weight on the quality of life.

[0080] It can be seen from Table 15 that compared with before taking, after taking the fat-reducing composition of Experimental Group 3 for one month, the role physical (RP) and general health (GH) in the SF-36 score can be significantly improved (p < 0.05). After taking it for two months, the physical function (PF), general health (GH) and health transition (HT) in the SF-36 score all increased significantly (p < 0.05). Among them, the physical function (PF) and health transition (HT) already showed an improving trend one month after taking, and showed a significant increase in the second month.

[0081] It can be seen from this embodiment that the fat-reducing composition provided by the present invention can effectively reduce body weight, BMI, waist circumference, hip circumference, as well as reduce the body fat percentage and subcutaneous fat thickness, and improve the quality of life of the subjects.

[0082] The above are only the preferred embodiments of the present invention and are not intended to limit the present invention. Any modifications, equivalent substitutions, improvements, etc. made within the spirit and principle of the present invention shall be included within the scope of protection of the present invention.

Claims

1. A fat-reducing composition, characterized in that, comprising seabuckthorn leaf extract; and a first mixed component and / or a second mixed component; wherein, the first mixed component comprises ampelopsis grossedentata extract and momordica grosvenori extract, and the second mixed component comprises a first instant coffee powder and a second instant coffee powder, and the second instant coffee powder contains chlorogenic acid.

2. The fat-reducing composition according to claim 1, wherein, The fat-reducing composition comprises the following components by mass: 2 to 12 parts by mass of seabuckthorn leaf extract; 1 to 13 parts by mass of ampelopsis grossedentata extract; 1 to 4 parts by mass of momordica grosvenori extract.

3. The fat-reducing composition according to claim 1, wherein The fat-reducing composition comprises the following components by mass: 2 to 12 parts by mass of seabuckthorn leaf extract; 9 to 33 parts by mass of the first instant coffee powder; 11 to 35 parts by mass of the second instant coffee powder.

4. The fat-reducing composition according to claim 1, characterized in that, The fat-reducing composition comprises the following components by mass: 2 to 12 parts by mass of seabuckthorn leaf extract; 1 to 13 parts by mass of ampelopsis grossedentata extract; 1 to 4 parts by mass of momordica grosvenori extract; 9 to 33 parts by mass of the first instant coffee powder; 11 to 35 parts by mass of the second instant coffee powder.

5. The fat-reducing composition according to any one of claims 1 to 4, characterized in that, The seabuckthorn leaf extract is 2 parts by mass or 8 parts by mass or 12 parts by mass, and the content of its polyphenolic compounds is not less than 20 wt% or not less than 25 wt%.

6. The fat-reducing composition according to any one of claims 1, 2 or 4, characterized in that, The ampelopsis grossedentata extract is 1 part by mass or 4 parts by mass or 13 parts by mass, and the content of its dihydromyricetin is not less than 30 wt% or not less than 40 wt%; The momordica grosvenori extract is 1 part by mass or 3 parts by mass or 4 parts by mass, and the content of its mogroside V is not less than 1 wt% or not less than 1.5 wt%.

7. The fat-reducing composition according to any one of claims 1, 3 or 4, characterized in that, The first instant coffee powder is 9 parts by mass or 26 parts by mass or 33 parts by mass; The second instant coffee powder is 11 parts by mass or 18 parts by mass or 35 parts by mass, and the content of its chlorogenic acid is 10 wt% - 15 wt% or 10 wt% - 12 wt% or 12 wt% - 15 wt% or 12 wt%.

8. The fat-reducing composition according to claim 1, wherein The seabuckthorn leaf extract is obtained by the following method: S1. Pretreatment of seabuckthorn leaves: After crushing the seabuckthorn leaves, sieve them for later use; S2. Preparation of extraction solution: Add pure water to the seabuckthorn leaves pretreated in step S1, the added mass of pure water is 10 - 20 times the mass of the seabuckthorn leaves, then heat the temperature of the mixed system to 70°C - 95°C, stir for 30 - 100 min, filter the liquid through a 50 - 200 mesh sieve to obtain the extraction solution; S3. Post-treatment of extraction solution: Centrifuge the extraction solution obtained in step S2 at a rotation speed of 2000 - 5000 rpm for 2 - 20 min, take the clarified liquid, concentrate the clarified liquid at 20 - 80°C and 40 - 70 mbar pressure until the Brix is 9 - 12%, then stop and perform UHT sterilization treatment to obtain the concentrated extraction solution; S4. Spray drying: Spray dry the concentrated extraction solution obtained in step S3 under the conditions of an inlet temperature of 155 - 185°C and an outlet temperature of 80 - 90°C to obtain the powdery seabuckthorn leaf extract.

9. Use of a fat-reducing composition according to any one of claims 1 - 4 in the preparation of a fat-reducing product.

10. The application according to claim 9, characterized in that, The fat-reducing product includes oral liquid, granule, powder, capsule or tablet.

11. A method for preparing a fat-reducing composition according to any one of claims 1 to 4, characterized in that, Mix the components of the fat-reducing composition evenly to obtain the fat-reducing composition.

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