Low-intensity intermittent stress model and device

Abstract

The invention relates to the field of model testing, in particular to a low-intensity intermittent stress model and device. According to the model and the device, a target body is observed within set time, the set time comprises five groups of time periods, and each group of time periods comprises three days. And through three-day stress observation of each group, the response of the corresponding excitation of the target body is obtained.

Description

Technical Field

[0001] The present invention relates to the field of model testing, and more particularly, to a model and device for low-intensity intermittent stress. Background Art

[0002] Currently, there are a large number of depression patients globally. Patients not only have a very poor life experience but also cause a huge socio-economic burden. As is well known, stress has two sides. Long-term major negative stress can lead to or trigger mental illnesses such as depression. However, low-intensity intermittent stress is beneficial for tempering the mind and promoting the establishment of self-confidence, thus overcoming difficulties and getting out of the haze. Consistent with the principle of extinction therapy for post-traumatic stress disorder, the use of low-intensity intermittent stress (LIIS) for the treatment of depression and its mechanism are not yet clear. Therefore, a model capable of experimentally testing mice under low-intensity intermittent stress is needed. Summary of the Invention

[0003] Embodiments of the present invention provide a model and device for low-intensity intermittent stress to provide a model of an object under low-intensity intermittent stress.

[0004] According to an embodiment of the present invention, a model for low-intensity intermittent stress is provided, including:

[0005] Observing the object within a set time, where the set time includes five groups of time periods, and each group of time periods includes three days;

[0006] On the first day, observe the social stress of the object and record the behavior of the object;

[0007] On the second day, observe the restraint stress of the object and record the behavior of the object;

[0008] On the third day, do not perform any intervention on the object and record the behavior of the object;

[0009] Repeat the above operations in groups of three days until the records of five groups of time periods of the object are completed.

[0010] In one embodiment, the social stress observation is:

[0011] Record the first object and the second object in the same environment;

[0012] Observe whether the object attacks another object in the same environment. If, within a preset time, the first object attacks the second object, then the first object is marked as aggressive.

[0013] In one embodiment, a sucrose preference test is performed on the object.

[0014] In one embodiment, the sucrose preference test includes:

[0015] Four groups of test sucrose preference subjects are set up, including an olfactory bulbectomy group, a sham operation group, an olfactory bulbectomy plus low-intensity intermittent stress group, and a sham operation plus low-intensity intermittent stress group.

[0016] A device for low-intensity intermittent stress includes:

[0017] A social stress module for observing the social stress of the subject on the first day and recording the behavior of the subject;

[0018] A restraint stress module for observing the restraint stress of the subject on the second day and recording the behavior of the subject;

[0019] A non-intervention recording module for not intervening in the subject on the third day and recording the behavior of the subject;

[0020] The above operations are repeated in groups of three days until the recording of five time periods of the subject is completed.

[0021] In one embodiment, the social stress module includes:

[0022] A first recording unit for recording a first subject and a second subject in the same environment;

[0023] A second recording unit for observing whether the subject attacks another subject in the same environment. If the first subject attacks the second subject within a preset time, the first subject is marked as aggressive.

[0024] In one embodiment, the device further includes:

[0025] A sucrose test module for performing a sucrose preference test on the subject.

[0026] In one embodiment, the sucrose test module includes:

[0027] A grouping setting unit for setting four groups of test sucrose preference subjects, including an olfactory bulbectomy group, a sham operation group, an olfactory bulbectomy plus low-intensity intermittent stress group, and a sham operation plus low-intensity intermittent stress group.

[0028] A computer-readable medium, the computer-readable storage medium stores one or more programs, and the one or more programs can be executed by one or more processors to implement the steps in the low-intensity intermittent stress model as described in any one of the above.

[0029] A terminal device includes: a processor, a memory, and a communication bus; a computer-readable program executable by the processor is stored on the memory;

[0030] The communication bus realizes the connection and communication between the processor and the memory;

[0031] When the processor executes the computer-readable program, it implements the steps in the model of low-intensity intermittent stress as described in any one of the above.

[0032] The model and device of low-intensity intermittent stress in the embodiments of the present invention observe the target body within a set time. Among them, the set time includes five groups of time periods, and each group of time periods includes three days. Through the stress observation for each group of three days, the response of the target body to stress can be obtained. Description of the Drawings

[0033] The drawings described herein are used to provide a further understanding of the present invention, and constitute a part of this application. The schematic embodiments of the present invention and their descriptions are used to explain the present invention and do not constitute an improper limitation to the present invention. In the drawings:

[0034] Figure 1 It is a flowchart of the model of low-intensity intermittent stress of the present invention;

[0035] Figure 2 It is a module diagram of the device of low-intensity intermittent stress of the present invention;

[0036] Figure 3 It is an analysis diagram of the sucrose preference test of the present invention;

[0037] Figure 4 It is a terminal device diagram of the present invention. Detailed Embodiments

[0038] In order to enable those skilled in the art to better understand the solution of the present invention, the technical solutions in the embodiments of the present invention will be clearly and completely described below in conjunction with the drawings in the embodiments of the present invention. Obviously, the described embodiments are only a part of the embodiments of the present invention, rather than all the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by those of ordinary skill in the art without creative efforts shall fall within the protection scope of the present invention.

[0039] It should be noted that the terms "first", "second", etc. in the description, claims and above-mentioned drawings of the present invention are used to distinguish similar objects and do not necessarily describe a specific order or sequence. It should be understood that such used data can be interchanged under appropriate circumstances so that the embodiments of the present invention described herein can be implemented in an order different from those illustrated or described herein. In addition, the terms "comprising" and "having" and any variations thereof are intended to cover non-exclusive inclusion. For example, a process, method, system, product or device comprising a series of steps or units does not necessarily limit to those steps or units clearly listed, but may include other steps or units not clearly listed or inherent to these processes, methods, products or devices.

[0040] Embodiment 1

[0041] According to an embodiment of the present invention, a model of low-intensity intermittent stress is provided. Refer to Figure 1 , including:

[0042] Observing the target body within a set time, wherein the set time includes five groups of time periods, and each group of time periods includes three days;

[0043] S100: Observe the social stress of the target body on the first day and record the behavior of the target body;

[0044] S200: Observe the restraint stress of the target body on the second day and record the behavior of the target body;

[0045] S300: Do not perform any intervention on the target body on the third day and record the behavior of the target body;

[0046] Repeat the above operations in groups of three days until the recording of five groups of time periods for the target body is completed.

[0047] The model and device of low-intensity intermittent stress in the embodiments of the present invention observe the target body within a set time, wherein the set time includes five groups of time periods, and each group of time periods includes three days. Through the stress observation of each group of three days, the response of the target body to stress can be obtained. Refer to Table 1

[0048]

[0049] Table 1

[0050] In an embodiment, the social stress observation is:

[0051] Record the first target body and the second target body in the same environment;

[0052] Observe whether a target object in the same environment attacks another target object. If a first target object attacks a second target object within a preset time, the first target object is marked as aggressive.

[0053] For example, the target is set as mice. Under social stress environment, mice marked as CD1 are housed in single cages for 7 days to develop territorial awareness, and C57BL / 6 mice are housed for four to five days to adapt to the environment.

[0054] To screen the aggressiveness of CD1 mice, place C57BL / 6 mice in the cage of CD1 mice for three minutes. If the CD1 mice attack more than five times and the attack latency is less than one minute, they are considered aggressive. If they are aggressive at least twice in 3 days, they can be used as attacking mice in the model.

[0055] The C57BL / 6 mice in the experimental group were placed in a CD1 mouse cage and isolated with a transparent acrylic plate with holes for 24 hours five to ten minutes after being challenged. New CD1 mice were replaced every day to avoid adaptation.

[0056] In one embodiment, the sugar water preference test comprises:

[0057] Four groups of test sugar water preference targets were set up, including olfactory bulbectomy group, sham operation group, olfactory bulbectomy plus low-intensity intermittent stress group and sham operation plus low-intensity intermittent stress group.

[0058] refer to Figure 3 The mice in the olfactory bulbectomy group showed a lower percentage of sugar water preference compared with the sham operation group, and the mice in the olfactory bulbectomy plus low-intensity intermittent stress group showed a higher percentage of sugar water preference compared with the mice in the simple olfactory bulbectomy group.

[0059] The model of this application can also be used to experiment with low-intensity intermittent stress on humans: specific stress tasks can be formulated according to the characteristics of specific patients, and such tasks are conducive to improving the patient's own value and ability. The total paradigm time is 30 days, with 2 days of stress each time and 1 day of interval, for a total of 10 cycles.

[0060]

[0061] This application constructs a low-intensity intermittent stress model from the perspective of adaptability to stress to reduce the sensitivity of depression to stress, thereby being used for the treatment of depression; this model paradigm is low-cost, simple, and highly operational, which is conducive to the promotion of future clinical trials.

[0062] Example 2

[0063] According to another embodiment of the present invention, a low-intensity intermittent stress device is provided. Figure 2 ,include:

[0064] Observe the target object within a set time, where the set time includes five groups of time periods, and each group of time periods includes three days;

[0065] The social stress module 100 is used to observe the social stress of the target object on the first day and record the behavior of the target object;

[0066] The restraint stress module 200 is used to observe the restraint stress of the target object on the second day and record the behavior of the target object;

[0067] The no-intervention recording module 300 is used to not perform any intervention on the target object on the third day and record the behavior of the target object;

[0068] Repeat the above operations in groups of three days until the recording of the five groups of time periods of the target object is completed.

[0069] The low-intensity intermittent stress model and device in the embodiments of the present invention observe the target object within a set time, where the set time includes five groups of time periods, and each group of time periods includes three days. Through the stress observation of each group of three days, the response of the target object to stress can be obtained.

[0070] In one embodiment, the social stress observation is as follows:

[0071] The first recording unit records the first target object and the second target object in the same environment;

[0072] The second recording unit observes whether the target object attacks another target object in the same environment. If the first target object attacks the second target object within a preset time, the first target object is marked as aggressive.

[0073] In one embodiment, the device further includes:

[0074] The sugar water test module is used to perform a sugar water preference test on the target object.

[0075] In one embodiment, the sugar water test module includes:

[0076] The grouping setting unit is used to set four groups of target objects for testing sugar water preference, including an olfactory bulbectomy group, a sham operation group, an olfactory bulbectomy plus low-intensity intermittent stress group, and a sham operation plus low-intensity intermittent stress group.

[0077] Reference Figure 3 , the mice in the olfactory bulbectomy group showed a lower percentage of sugar water preference compared to those in the sham operation group, and the mice in the olfactory bulbectomy plus low-intensity intermittent stress group showed a higher percentage of sugar water preference compared to the mice in the simple olfactory bulbectomy group.

[0078] Example 3

[0079] Based on the above model of low-intensity intermittent stress, this embodiment provides a computer-readable storage medium storing one or more programs that can be executed by one or more processors to implement the steps in the model of low-intensity intermittent stress as described in the above embodiment.

[0080] Embodiment 4

[0081] A terminal device includes: a processor, a memory, and a communication bus; a computer-readable program executable by the processor is stored on the memory; the communication bus enables connection communication between the processor and the memory; when the processor executes the computer-readable program, the steps in the above model of low-intensity intermittent stress are implemented.

[0082] Based on the above model of low-intensity intermittent stress, this application provides a terminal device, as Figure 4 shown, which includes at least one processor 20; a display screen 21; and a memory 22, and may further include a communication interface 23 and a bus 24. Among them, the processor 20, the display screen 21, the memory 22, and the communication interface 23 can complete mutual communication through the bus 24. The display screen 21 is set to display a user guidance interface preset in the initial setting mode. The communication interface 23 can transmit information. The processor 20 can call the logical instructions in the memory 22 to execute the method in the above embodiment.

[0083] In addition, when the logical instructions in the above memory 22 are implemented in the form of a software functional unit and sold or used as an independent product, they can be stored in a computer-readable storage medium.

[0084] The memory 22, as a computer-readable storage medium, can be set to store software programs and computer-executable programs, such as the program instructions or modules corresponding to the method in the embodiments of the present disclosure. The processor 20 executes functional applications and data processing by running the software programs, instructions, or modules stored in the memory 22, that is, implements the method in the above embodiment.

[0085] The memory 22 may include a program storage area and a data storage area. The program storage area may store an operating system and application programs required for at least one function. The data storage area may store data created according to the use of the terminal device and the like. In addition, the memory 22 may include a high-speed random access memory and may also include a non-volatile memory. For example, various media that can store program codes, such as a USB flash drive, a mobile hard disk, a read-only memory (ROM), a random access memory (RAM), a magnetic disk, or an optical disc, may also be a transient storage medium.

[0086] In addition, the specific processes of loading and executing multiple instructions in the above-mentioned storage medium and the terminal device have been described in detail in the above method and will not be elaborated here one by one.

[0087] The above are only the preferred embodiments of the present invention. It should be noted that for those of ordinary skill in the art, without departing from the principle of the present invention, several improvements and refinements can be made, and these improvements and refinements should also be regarded as the protection scope of the present invention.

Claims

1. A model of low-intensity intermittent stress, characterized in that, Comprising: Observing the target body within a set time, wherein the set time includes five groups of time periods, and each group of the time periods includes three days; On the first day, observing the social stress of the target body and recording the behavior of the target body; On the second day, observing the restraint stress of the target body and recording the behavior of the target body; On the third day, no intervention is performed on the target body, and the behavior of the target body is recorded; Repeat the above operations in groups of three days until the recording of the five groups of time periods for the target body is completed.

2. The model of low-intensity intermittent stress according to claim 1, wherein The social stress observation is as follows: Recording the first target body and the second target body in the same environment; Observing whether the target body attacks another target body in the same environment. If within a preset time, the first target body attacks the second target body, then the first target body is marked as aggressive.

3. The model of low-intensity intermittent stress according to claim 1, wherein Conducting a sucrose preference test on the target body.

4. The model of low-intensity intermittent stress according to claim 3, wherein The sucrose preference test includes: Setting four groups of target bodies for testing sucrose preference, including an olfactory bulbectomy group, a sham operation group, an olfactory bulbectomy plus low-intensity intermittent stress group, and a sham operation plus low-intensity intermittent stress group.

5. A device for low-intensity intermittent stress, characterized in that, Comprising: A social stress module for observing the social stress of the target body on the first day and recording the behavior of the target body; A restraint stress module for observing the restraint stress of the target body on the second day and recording the behavior of the target body; A no-intervention recording module for not performing any intervention on the target body on the third day and recording the behavior of the target body; Repeat the above operations in groups of three days until the recording of the five groups of time periods for the target body is completed.

6. The device for low-intensity intermittent stress according to claim 1, characterized in that The social stress module includes: A first recording unit for recording the first target body and the second target body in the same environment; A second recording unit for observing whether the target body attacks another target body in the same environment. If within a preset time, the first target body attacks the second target body, then the first target body is marked as aggressive.

7. The device for low-intensity intermittent stress according to claim 1, wherein The device further includes: A sucrose test module for conducting a sucrose preference test on the target body.

8. The device for low-intensity intermittent stress according to claim 7, characterized in that, The sucrose test module includes: A grouping setting unit for setting four groups of target bodies for testing sucrose preference, including an olfactory bulbectomy group, a sham operation group, an olfactory bulbectomy plus low-intensity intermittent stress group, and a sham operation plus low-intensity intermittent stress group.

9. A computer-readable medium, characterized in that, The computer-readable storage medium stores one or more programs, and the one or more programs can be executed by one or more processors to implement the steps in the model of low-intensity intermittent stress as described in any one of claims 1-4.

10. A terminal device, characterized in that, Comprising: A processor, a memory, and a communication bus; The memory stores a computer-readable program executable by the processor; The communication bus realizes the connection and communication between the processor and the memory; When the processor executes the computer-readable program, it implements the steps in the model of low-intensity intermittent stress as described in any one of claims 1-4.

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