A camellia sinensis flower composition for increasing sebum content and its application in dry sensitive skin

Abstract

The application discloses a camellia sinensis var. assamica flower composition for improving sebum content and application thereof in dry sensitive skin, and relates to the technical field of cosmetics.The application provides a camellia sinensis var. assamica flower composition, which comprises the following components in parts by weight: camellia sinensis var. assamica flower extract 1-8 parts, ophiopogon root extract 0.5-5 parts, sweet almond seed extract 0.5-8 parts and asaai oil 3-10 parts.The camellia sinensis var. assamica flower composition of the application selects and adds the camellia sinensis var. assamica flower extract, the ophiopogon root extract, the sweet almond seed extract and the asaai oil with proper parts by weight, and the four components can synergize with each other, effectively play the effects of improving epidermal sebum content, improving brightness and soothing skin, and further slow down skin discomfort.

Description

Technical Field

[0001] The present invention relates to the technical field of cosmetics, in particular to a golden camellia composition for increasing sebum content and application thereof to dry and sensitive skin. Background Art

[0002] The core structure of the skin barrier system, the epidermal lipid film, is a dynamic protective membrane structure formed by the emulsification of sebaceous gland secretions and sweat. This biological membrane system plays multiple physiological functions through its unique three-phase structure (lipid phase, water phase, liquid crystal phase): ① forming a selective permeability barrier to regulate transepidermal water loss; ② maintaining a weakly acidic environment on the skin surface; ③ constituting an innate immune defense system; and ④ mediating epidermal homeostasis regulation. In the pathological state of dry and sensitive skin, the structural integrity of the sebum film is damaged, which will trigger a cascade reaction: the dysfunction of the epidermal permeability barrier leads to a decrease in the hydration capacity of the stratum corneum and an abnormal increase in the transepidermal water loss rate, thereby inducing neurogenic inflammatory reactions and immune response disorders. Existing technical means mainly focus on the following two directions: (1) Lipid supplementation therapy: by exogenously supplementing physiological lipid components such as ceramide, cholesterol and free fatty acids, an attempt is made to rebuild the epidermal lipid bilayer structure. However, clinical studies have shown that simply promoting the synthesis of lamellar body lipids cannot effectively regulate their exocytosis and extracellular matrix reorganization process. In vitro experiments have confirmed that overloaded ceramide in keratinocytes activates the TLR2 / NF-κB pathway, leading to upregulation of pro-inflammatory factors such as IL-6 and TNF-α, forming a vicious cycle of lipid metabolism disorders and inflammatory responses. (2) Hydration enhancement strategy: Use macromolecular moisturizers such as sodium hyaluronate and β-glucan to construct an epidermal water absorption network. However, in vivo confocal microscopy observations found that in the state of sebum membrane defects, these hydrophilic macromolecules can only be distributed in the upper part of the stratum corneum and cannot penetrate the complete lamellar membrane structure. Molecular dynamics simulations show that the increased hydrodynamic osmotic gradient on the surface of damaged skin will cause water to diffuse in reverse, ultimately shortening the hydration half-life to 1 / 3 of that of normal skin. This "permeation trap" phenomenon makes it difficult for moisturizers to achieve a sustained water release function.

[0003] In other words, existing solutions fail to solve the problem of dynamic balance of lipid transport and secretion, especially the lack of regulation of ceramide transporter activity; they fail to improve the problem of skin physiological rhythm disrupted by increased inflammation levels in dry and sensitive skin, resulting in obstruction of skin cell growth, metabolism and material transport functions, and skin system disorder or delayed recovery.

[0004] In view of this, this application is filed. Summary of the Invention

[0005] Based on this, the purpose of the present invention is to overcome the shortcomings of the above-mentioned prior art and provide a golden camellia composition for increasing sebum content and its application in dry and sensitive skin.

[0006] To achieve the above-mentioned purpose, the technical solution adopted by the present invention is: a golden camellia composition, comprising the following components in parts by weight: 1-8 parts of golden camellia extract, 0.5-5 parts of vetiver root extract, 0.5-8 parts of sweet almond seed extract, and 3-10 parts of acai oil.

[0007] Sweet almond seed extract contains a variety of fatty acids, among which unsaturated fatty acids can upregulate the expression of fatty acid transporters and promote the targeted accumulation of long-chain fatty acids in lamellar bodies. Saturated fatty acids also directly replenish and rebuild the sebum membrane, effectively promoting the biological function of repairing the cell sebum membrane and protecting the skin from external aggressors. The unsaturated fatty acids and polyphenols contained in acai oil can effectively regulate the expression level of ceramide transporters, preventing endoplasmic reticulum stress caused by lipid overload. The two can synergize to form a complementary fatty acid profile, ensuring the integrity and stability of the sebum membrane. The flavonoid glycosides contained in golden camellia extract can promote cell membrane fluidity and significantly enhance the exocytosis efficiency of lamellar bodies. The sesquiterpenes contained in vetiver root extract can promote the release of more lipid components from lamellar bodies, filling the intercellular spaces and rapidly repairing the sebum barrier. The two can work together to achieve the dual optimization of promoting transporter activity and improving the exocytosis efficiency of lamellar bodies, fully strengthening the sebum barrier function. The combination of the four substances provided by the present invention can synergistically exert soothing and moisturizing effects, and has a significant effect on repairing the sebum film. It can not only increase the sebum content, but also effectively transport lipids to the epidermis, solving the problem of dynamic imbalance in lipid transport and secretion.

[0008] At the same time, the unsaturated fatty acids in sweet almond seed extract and acai oil can also restore the transcription levels of circadian clock genes. The cyclic sesquiterpenes in vetiver root extract can regulate the circadian clock cycle, returning the epidermal lipid metabolism rhythm to normal and avoiding the excessive thickness of the sebum film caused by premature activation of lipid synthesis. The combination of the four substances provided by this invention can also improve skin rhythm dysfunction, reverse the impaired expression levels of skin rhythm genes, and enable skin cells to perform their biological functions in an orderly and stable manner.

[0009] The inventors found in actual research that by adding appropriate weight portions of Camellia chrysantha extract, Vetiver root extract, Prunus amygdalus seed extract and Acai oil to the Camellia chrysantha composition of the present invention, the four components can work together to effectively increase the epidermal sebum content, enhance brightness and soothe the skin, further alleviating skin discomfort.

[0010] Preferably, the Camellia chrysantha composition comprises the following components in parts by weight: 3-6 parts of Camellia chrysantha extract, 1-3 parts of Vetiver root extract, 2-5 parts of Prunus amygdalus seed extract, and 6-9 parts of Acai oil.

[0011] The inventors found in actual research that the weight of the components in the Camellia chrysantha composition provided by the present invention will affect the performance of the product. When the weight of the components is further selected to be within the above-mentioned range, the dynamic balance of lipid transport and secretion of the obtained product is better; it can better restore the skin's physiological rhythm to normal, reverse the damaged expression of skin rhythm genes, and enable skin cells to perform biological functions in an orderly and stable manner; the obtained product is better in increasing the epidermal sebum content, improving brightness, and soothing the skin.

[0012] Preferably, the weight percentage of the sweet almond seed extract is 15-25% based on the total weight of the Camellia chrysantha composition.

[0013] Preferably, the weight percentage of acai oil is 40-50% based on the total weight of the Camellia chrysantha composition.

[0014] The inventors discovered in actual research that the weight percentage of the sweet almond seed extract and the weight percentage of the acai oil, based on the total weight of the Camellia chrysantha composition, will affect the performance of the product. When the weight percentages of the components are further selected within the above range, the resulting product has a better dynamic balance effect on lipid transport and secretion; it can better restore the skin's physiological rhythm to normal, reverse the damaged expression of skin rhythm genes, and enable skin cells to perform their biological functions in an orderly and stable manner; the resulting product is more effective in increasing epidermal sebum content, enhancing brightness, and soothing the skin.

[0015] Preferably, the preparation method of the sweet almond seed extract of the present invention is as follows:

[0016] The sweet almond seeds are soaked, ground, sieved, and subjected to supercritical CO2 extraction to obtain the sweet almond seed extract.

[0017] Preferably, the preparation method of the sweet almond seed extract comprises at least one of the following:

[0018] (1) The sweet almond seeds are soaked for 1-5 hours and sieved with a mesh size of 15-20;

[0019] (2) In the supercritical CO2 extraction, the extraction temperature is 37-42°C and the extraction pressure is 15-30 MPa;

[0020] (3) In the supercritical CO2 extraction, the carbon dioxide flow rate is 10-30 mL min -1 , the extraction time is 50-70min.

[0021] The inventors discovered in actual research that the sweet almond seed extract prepared using the above-mentioned sweet almond seed extract preparation method satisfies a specific weight ratio of palmitic acid, oleic acid, and linoleic acid, which further influences the effectiveness of the resulting product. When the weight ratio is within the above-mentioned range, the resulting product achieves a better dynamic balance of lipid transport and secretion; it can also better restore skin circadian rhythms, reverse impaired expression of skin rhythm genes, and enable skin cells to perform their biological functions in an orderly and stable manner. The resulting product is also more effective in increasing epidermal sebum content, enhancing brightness, and soothing the skin.

[0022] Preferably, during the supercritical CO2 extraction, silicon dioxide and sweet almond seeds are mixed for extraction, and the weight ratio of silicon dioxide to sweet almond seeds is (0.5-2):1.

[0023] In addition, the present invention provides the use of the Camellia chrysantha composition in preparing cosmetics.

[0024] Furthermore, the present invention provides a cosmetic comprising the Camellia chrysantha composition. Preferably, the cosmetic comprises any one of a toner, lotion, ointment, cream, mask, essence, and spray.

[0025] Preferably, the cosmetic comprises the following components in percentage by mass: 0.5-3% of the Camellia chrysantha composition, 4-40% of the cosmetic base, and the balance being water.

[0026] Preferably, the cosmetic matrix includes at least one of a thickener, a moisturizer, an emulsifier, a preservative, a fragrance, and a pH adjuster.

[0027] Preferably, the cosmetic comprises the following components in mass percentage: 0.5-3% of Camellia chrysantha composition, 0.05-0.5% of thickener, 3-20% of moisturizer, 0.5-10% of emulsifier, 0.2-5% of preservative, 0.01-0.2% of flavor, 0.05-0.1% of pH adjuster, and the balance is water.

[0028] Exemplarily, the thickener includes at least one of xanthan gum, carbomer 980, AVC, and high molecular weight cellulose; the moisturizer includes at least one of glycerin, butylene glycol, and low molecular weight sodium hyaluronate; the emulsifier includes at least one of coconut oil-caprylate / caprate, polydimethylsiloxane, hydrogenated palm kernel oil, cetearyl alcohol, polyglyceryl-6 distearate, and polymethylsilsesquioxane; the preservative includes at least one of 1,3-propylene glycol, 1,2-hexanediol, and parahydroxyacetophenone; the pH adjuster includes at least one of arginine and EDTA-2Na.

[0029] Compared with the prior art, the present invention has the following beneficial effects:

[0030] The combination of the four substances provided by the present invention can synergistically exert soothing and moisturizing effects, and has a significant effect on repairing the sebum film. It can not only increase the sebum content, but also effectively transport lipids to the epidermis, solving the dynamic balance problem of lipid transport and secretion.

[0031] The combination of the four substances provided by the present invention can also restore the skin's physiological rhythm to normal, reverse the damaged expression of skin rhythm genes, and enable skin cells to perform their biological functions in an orderly and stable manner.

[0032] The inventors found in actual research that by adding appropriate weight portions of Camellia chrysantha extract, Vetiver root extract, Prunus amygdalus seed extract and Acai oil to the Camellia chrysantha composition of the present invention, the four components can work together to effectively increase the epidermal sebum content, enhance brightness and soothe the skin, further alleviating skin discomfort. BRIEF DESCRIPTION OF THE DRAWINGS

[0033] Figure 1 (A)-(C) are fluorescence images of the expression levels of ceramide transporter CERT in the blank control group, Example 1, and Comparative Example 13, respectively;

[0034] Figure 2 (A)-(C) are graphs showing the promoting effects of the blank application example, application example 1 and comparative application example 13 on the epidermal sebum film content on the 14th day. DETAILED DESCRIPTION

[0035] In order to better illustrate the purpose, technical solutions and advantages of the present invention, the present invention will be further described below with reference to the accompanying drawings and specific examples. Its purpose is to understand the content of the present invention in detail, rather than to limit the present invention. All other embodiments obtained by ordinary technicians in this field without making creative work are within the scope of protection of the present invention. Unless otherwise specified, the experimental reagents and instruments designed for the implementation and comparative examples of the present invention are all commonly used ordinary reagents and instruments, which can be obtained from commercial channels. In the implementation and comparative examples, the experimental methods used are all conventional methods unless otherwise specified; and unless otherwise specified, the raw materials used in parallel experiments are from the same batch of raw materials.

[0036] The raw materials of the present invention are now further described, but are not limited to the following raw materials:

[0037] Camellia chrysantha extract: purchased from Ximuyuan (Xiamen) Biotechnology Co., Ltd.

[0038] Vetiver root extract: purchased from Ningxia Vanilla Biotechnology Co., Ltd.

[0039] Acai oil: purchased from Xianting (Guangzhou) Technology Research and Development Co., Ltd.

[0040] Bifolia jasminoides extract: purchased from Shanghai MacLean Biochemical Technology Co., Ltd.

[0041] Licorice root extract: purchased from Xi'an Perfe Biotechnology Co., Ltd.

[0042] Redwood seed extract: purchased from Shaanxi Hongpeptide Biotechnology Co., Ltd.;

[0043] Olive oil: purchased from Shanghai Jingchun Biotechnology Co., Ltd.

[0044] Sweet almond seed extract-1: Homemade, the preparation method includes the following steps:

[0045] Soak sweet almond seeds for 2.5 hours and grind them through an 18-mesh sieve. Mix silicon dioxide and sweet almond seeds in a ratio of 1:1 and put them into an extraction kettle. Place the extraction kettle in a supercritical fluid and set the extraction temperature to 37°C, the extraction pressure to 20 MPa, and the CO2 flow rate to 25 mL min. -1 The extract was collected at the outlet using a test tube filled with n-hexane. The extraction was stopped after 1 hour. The n-hexane was removed by rotary evaporation at 35°C to obtain the sweet almond seed extract.

[0046] Sweet almond seed extract-2: homemade. The difference between the preparation method and sweet almond seed extract-1 lies in the different parameters during the extraction process. Specifically, the extraction temperature is set at 42°C, the extraction pressure is 30 MPa, and the CO2 flow rate is 15 mL min -1 .

[0047] Sweet almond seed extract-3: homemade. The difference between the preparation method and sweet almond seed extract-1 lies in the different parameters in the extraction process, specifically: setting the extraction temperature to 32°C and the extraction pressure to 40Mpa.

[0048] Sweet almond seed extract-4: homemade. The difference between the preparation method and sweet almond seed extract-1 lies in the different parameters in the extraction process, specifically: setting the extraction temperature to 45°C and the extraction pressure to 10Mpa.

[0049] Sweet almond seed extract-5: Homemade, the preparation method is as follows: wash the sweet almond seeds and dry them in an oven at 60℃, grind them through a 40-mesh sieve, add n-hexane at a material-liquid ratio of 1:6 at room temperature, ultrasonicate at 40℃ and 100w for 30 minutes, then extract at room temperature for 3 hours, and obtain sweet almond seed extract-5 by filtration and rotary evaporation at 35℃.

[0050] Examples and Comparative Examples

[0051] The embodiments of the present invention and the comparative examples provide a Camellia chrysantha composition, the components and weight portions of the composition are shown in Tables 1 and 2, W1 represents the weight percentage of the sweet almond seed extract based on the total weight of the Camellia chrysantha composition, and W2 represents the weight percentage of the acai oil based on the total weight of the Camellia chrysantha composition; the preparation method of the composition comprises the following steps: uniformly mixing the components to obtain the Camellia chrysantha composition.

[0052] Table 1

[0053]

[0054]

[0055] Table 2

[0056]

[0057] Application Examples 1-11, Comparative Application Examples 1-14, and Blank Application Examples

[0058] The application examples, comparative application examples and blank application examples of the present invention provide an emulsion, and the components (mass percentage) of the emulsion are shown in Table 3; wherein, the chrysantha camellia compositions used in application examples 1-10 are respectively the chrysantha camellia compositions prepared in embodiments 1-10, for example, the chrysantha camellia composition in embodiment 1 is used in application example 1, the chrysantha camellia composition in embodiment 2 is used in application example 2, and so on; the chrysantha camellia compositions used in comparative application examples 1-14 are respectively the chrysantha camellia compositions prepared in comparative examples 1-14; the chrysantha camellia composition used in application example 11 is the chrysantha camellia composition prepared in embodiment 1.

[0059] Table 3

[0060]

[0061] The preparation method of the emulsion provided in Application Example 1 comprises the following steps:

[0062] (1) Mix phase A with water, heat to 80°C, and homogenize at 1200 rpm for 5 minutes. After homogenization, keep warm and set aside to obtain prefabricated phase A;

[0063] (2) Mix phase B, heat to 80°C, and homogenize at 1200 rpm for 5 min. After homogenization, keep warm and set aside to obtain prefabricated phase B;

[0064] (3) Mix phase C and heat to 60°C to melt to obtain prefabricated phase C;

[0065] (4) Heat the preformed phase A to 80°C, add the preformed phase B at a speed of 300 rpm, stir and mix, then cool to 60°C, add the preformed phase C at a speed of 300 rpm, stir and mix, then cool to below 45°C, add the composition and flavor, and continue stirring for 5 minutes. Finally, add arginine to adjust the pH to 5.0-6.5, then stop stirring, discharge, and obtain an emulsion.

[0066] The preparation methods of the emulsions provided in Application Examples 2-11, Comparative Application Examples 1-14 and Blank Application Examples are consistent with that in Application Example 1, and no relevant components are added if they are not present.

[0067] Performance Test-1

[0068] CERT is a lipid transporter that transports ceramide from the endoplasmic reticulum (ER) to the trans-Golgi apparatus, a prerequisite for the conversion of ceramide to sphingomyelin. This effect example explores the enhancing effect of the Camellia chrysantha compositions provided in Examples 1-10 and Comparative Examples 1-14 of the present invention on lipid transporters.

[0069] Experimental content:

[0070] (1) Cell culture and treatment: Keratinocytes were cultured at a rate of 5 × 10 4 Cells were seeded in 24-well plates containing collagen-coated slides (50 μg / mL). After 48 hours of culture, the sample group was stimulated for 5 days by adding a 1% (v / v) concentration of the composition (diluted with DMSO, with a final DMSO concentration of 0.5%). The blank control group was supplemented with cell culture medium.

[0071] (2) Immunocytofluorescence analysis: After treatment, cells were rinsed twice with sterile PBS, then 2% paraformaldehyde containing 0.5% Triton×100 was added, and the cells were permeabilized with the primary antibody (anti-CERT / COL4A3BP rabbit polyclonal antibody) for 5 minutes and fixed with 2% paraformaldehyde for 15 minutes. After fixation, cells were rinsed twice with sterile PBS, and nonspecific sites were blocked with 3% bovine serum albumin and 0.1% Tween 20 for 1 hour. The primary antibody was diluted 1000 times in blocking solution (3% BSA + 0.1% Tween 20) and incubated with cells in the dark at 4°C overnight. Finally, cells were rinsed three times with PBS and 0.3% Triton×100 and incubated with the secondary antibody (Goat anti-rabbit Alexa Fluor 488) at room temperature in the dark for 2 hours. Finally, cells were rinsed three times with PBS and 0.3% Triton×100, rinsed twice with sterile PBS, and stained with DAPI. Fluorescence images were collected and observed using a fluorescence microscope, and the fluorescence intensity was quantified using Image J software.

[0072] (3) CERT enhancement rate (%) = |CERT fluorescence intensity of sample group - CERT fluorescence intensity of blank group| / CERT fluorescence intensity of blank group × 100%.

[0073] (4) The experimental results are shown in Table 4.

[0074] Table 4

[0075]

[0076]

[0077] As can be seen from the above table, the CERT improvement rate of the Camellia chrysantha composition prepared in the embodiment of the present invention is more than 100%. Figure 1 (A)-(C) are fluorescence images of the expression levels of ceramide transporter protein CERT in the blank control group (A), Example 1 (B) and Comparative Example 13 (C), respectively. The Camellia chrysantha composition prepared in the embodiment of the present invention has a good enhancing effect on lipid transporter protein.

[0078] From the comparison of Examples 1-6, it can be seen that the weight of the components in the Camellia chrysantha composition provided by the present invention will affect the performance of the product. When the weight of the components is further selected within the preferred range, the obtained composition has a better effect on enhancing lipid transport proteins and a better dynamic balance effect on lipid transport and secretion.

[0079] From the comparison of Example 1 and Examples 7-10, it can be seen that when the sweet almond seed extracts prepared by different sweet almond seed extract preparation methods are added to the golden camellia composition, the enhanced effect of the obtained golden camellia composition on lipid transport protein will be affected, thereby affecting the dynamic balance effect of lipid transport and secretion.

[0080] As can be seen from Example 1 and Comparative Examples 1-8, when one or two of the components are not added, or when they are not added and the amount is supplemented with other components, the effects of the present invention cannot be achieved; as can be seen from Example 1 and Comparative Examples 9-12, when other similar components are substituted, the corresponding effects of the present invention cannot be achieved. As can be seen from Example 1 and Comparative Examples 13-14, when the weight proportions of the components in the composition are not within the range of this application, a good synergistic effect cannot be achieved.

[0081] Performance test-2 clock gene clock test

[0082] This effect example explores the effect of the Camellia chrysantha composition provided by Examples 1-10 and Comparative Examples 1-14 of the present invention on the clock gene clock.

[0083] 1. Experimental materials:

[0084] (1) Cell line: HaCaT cells, source: Guangzhou Customs Technology Center, passage number: 14.

[0085] (2) Culture medium: MEM medium containing 10% fetal bovine serum and 1% antibiotics (FBS, Gibco, Lot: 2500251P)

[0086] (3) Culture conditions: incubator temperature 37°C, humidity 90%, CO2 5%.

[0087] (4) Experimental content: When the cell confluence reached 70%, clock gene synchronization was achieved by serum starvation for 24 hours. After synchronization, the sample group treated the cells with 50 ng / mL TNF-α and 0.06% Camellia chrysantha combination (m / m) in EMEM containing 2% fetal bovine serum for 24 hours before RNA extraction, the model group treated the cells with 50 ng / mL TNF-α for 24 hours before RNA extraction, and the blank group cultured the cells normally for 24 hours before RNA extraction. The clock gene clock (Hs04546767_m1) was detected using the StepOne real-time fluorescence quantitative PCR system. β-actin (Hs99999903_m1) was used as a reference gene, and the clock mRNA level was normalized to the β-actin mRNA level to calculate the gene expression level. The 2^-(ΔΔCt) method was used to quantify the relative expression content of clock gene mRNA.

[0088] Clock enhancement rate (%) = |Relative expression level of clock mRNA in the sample group - Relative expression level of clock mRNA in the model group| / Relative expression level of clock mRNA in the model group × 100%.

[0089] (6) The experimental results are shown in Table 5:

[0090] Table 5

[0091]

[0092]

[0093] As can be seen from the above table, the CLOCK enhancement rate of the Camellia chrysantha composition prepared in the embodiment of the present invention reaches more than 35%, which restores the skin physiological rhythm to normal, reverses the damaged expression of skin rhythm genes, and enables skin cells to perform biological functions in an orderly and stable manner.

[0094] From the comparison of Examples 1-6, it can be seen that the weight of the components in the Camellia chrysantha composition provided by the present invention will affect the performance of the product. When the weight of the components is further selected within the preferred range, the obtained composition can better restore the skin's physiological rhythm to normal, reverse the damaged expression of skin rhythm genes, and enable skin cells to perform their biological functions in an orderly and stable manner.

[0095] From the comparison of Example 1 and Examples 7-10, it can be seen that when the sweet almond seed extracts prepared by different sweet almond seed extract preparation methods are added to the golden camellia composition, the performance of the obtained golden camellia composition will be affected. The specific preparation method of the present invention can better restore the skin's physiological rhythm to normal, reverse the damaged expression of skin rhythm genes, and enable skin cells to perform biological functions in an orderly and stable manner.

[0096] As can be seen from Example 1 and Comparative Examples 1-8, when one or two of the components are not added, or when they are not added and the amount is supplemented with other components, the effects of the present invention cannot be achieved; as can be seen from Example 1 and Comparative Examples 9-12, when other similar components are substituted, the corresponding effects of the present invention cannot be achieved. As can be seen from Example 1 and Comparative Examples 13-14, when the weight proportions of the components in the composition are not within the range of this application, a good synergistic effect cannot be achieved.

[0097] Performance Test-3

[0098] This effect example tests the improvement effects of the application example, comparative application example, and blank application example on the human epidermal sebum content, brightness, and skin redness. The specific testing method is as follows:

[0099] According to the "Technical Specifications for Safety of Cosmetics" (2015), volunteers aged 18-45 with dry and sensitive skin (lactic acid stinging score > 3, skin moisture content 20C.U~50C.U) were selected and randomly divided into 26 groups, with 5 people in each group. The subjects washed their faces with clean water without applying any products. They sat in an air-conditioned room with a temperature of 21±1℃ and a humidity of 50±10% for 20 minutes to test their skin condition before use.

[0100] Every morning and evening, after cleansing and moisturizing, volunteers applied an appropriate amount of the lotion to their entire face, massaging until absorbed. This was done once each morning and evening, with follow-up visits scheduled on days 1 and 14. On the day of their visit, volunteers washed their faces with clean water without applying any other products. They sat quietly in an air-conditioned room at a temperature of 21±1°C and a humidity of 50±10% for 20 minutes. Improvements in facial indicators were quantified and photographed using instruments.

[0101] These included the Sebumeter SM 815 for testing epidermal sebum content, the Skin-Glossymeter GL 200 for testing skin brightness, and the Skin-Colorimeter CL400 and Visia 7 (IPP) for testing skin redness.

[0102] Improvement rate before and after use = |X 使用前均值 -X 使用后均值 | / X 使用前均值 ×100%;

[0103] The test results are shown in Table 6.

[0104] Table 6

[0105]

[0106]

[0107] As can be seen from the above table, the emulsion prepared in the application example of the present invention has a good effect of increasing the epidermal sebum content, improving brightness and removing redness. Figure 2 (A)-(C) show the effects of blank application example, application example 1, and comparative application example 13 on promoting epidermal sebum membrane content. In dry and sensitive skin, sebum cannot be effectively transported to the epidermis after synthesis to replenish the sebum membrane and nourish the skin. The composition provided by the present invention can effectively promote lipid transport, achieve rapid epidermal sebum replenishment, and produce significant brightening and redness-removing effects. The emulsion prepared in the application example of the present invention takes effect quickly after use, and significant effects can be seen after one day of use.

[0108] From the comparison of Application Examples 1-6, it can be seen that the weight of the components in the composition will affect the performance of the product. When the Camellia chrysantha composition includes the following components in weight: 3-6 parts of Camellia chrysantha extract, 1-3 parts of Vetiver root extract, 2-5 parts of Prunus amygdalus seed extract, and 6-9 parts of Acai oil, it has better effects on increasing epidermal sebum content, improving brightness and removing redness.

[0109] From the comparison of Application Example 1 and Application Examples 7-10, it can be seen that the efficacy of the Camellia chrysantha composition obtained by preparing the sweet almond seed extract using different extraction methods will vary. When preparing the emulsion, there will be obvious differences in the effects of increasing the epidermal sebum content, improving brightness, and removing redness. When the sweet almond seed extract is prepared by supercritical CO2 extraction, the effects of increasing the epidermal sebum content, improving brightness, and soothing the skin are better. When supercritical CO2 extraction is used, and the extraction temperature is 37-42°C, the extraction pressure is 15-30Mpa, and the carbon dioxide flow rate is 10-30mL·min -1 When used, it has better effects on increasing epidermal sebum content, improving brightness and soothing the skin.

[0110] It can be seen from Application Example 1 and Comparative Application Examples 1-8 that when one or two of the components are not added to the composition, or when they are not added and the other components are used to make up the difference, the effect of the present invention cannot be achieved; it can be seen from Application Example 1 and Comparative Application Examples 9-12 that when other similar ingredients are used instead, the corresponding effect of the present invention cannot be achieved; it can be seen from Application Example 1 and Comparative Application Examples 13-14 that when the specific four ingredients of the present invention are used, but the weight ratio is not within the specific range of the present invention, the effects of increasing the epidermal sebum content, increasing brightness and removing redness cannot be achieved; the four components of the present invention work together and none of them can be missing.

[0111] Finally, it should be noted that the above embodiments are only used to illustrate the technical solutions of the present invention and are not intended to limit the scope of protection of the present invention. Although the present invention has been described in detail with reference to preferred embodiments, those skilled in the art should understand that the technical solutions of the present invention may be modified or replaced by equivalents without departing from the essence and scope of the technical solutions of the present invention.

Claims

1. A golden camellia composition, characterized in that The invention comprises the following components in parts by weight: 3-6 parts of golden camellia flower extract, 1-3 parts of vetiver root extract, 2-5 parts of sweet almond seed extract, and 6-9 parts of acai oil; the preparation method of the sweet almond seed extract is as follows: sweet almond seeds are soaked, ground, sieved, and subjected to supercritical CO2 extraction to obtain the sweet almond seed extract; in the supercritical CO2 extraction, the extraction temperature is 37-42°C, and the extraction pressure is 15-30 MPa.

2. The golden camellia composition according to claim 1, characterized in that Based on the total weight of the golden camellia composition, the weight percentage of the sweet almond seed extract is 15-25%.

3. The golden camellia composition according to claim 1, wherein Based on the total weight of the golden camellia composition, the weight percentage of acai oil is 40-50%.

4. The golden camellia composition according to claim 1, wherein Include at least one of the following: (1) The sweet almond seeds are soaked for 1-5 hours and sieved with a mesh size of 15-20; (2) In the supercritical CO2 extraction, the carbon dioxide flow rate is 10-30 mL min -1 , the extraction time is 50-70min.

5. Use of the Camellia chrysantha composition according to any one of claims 1 to 4 in the preparation of cosmetics.

6. A cosmetic, characterized in that: The cosmetic comprises the Camellia chrysantha composition according to any one of claims 1 to 4.

7. The cosmetic according to claim 6, wherein The cosmetic comprises the following components in percentage by mass: 0.5-3% of golden camellia composition, 4-40% of cosmetic base, and the balance of water.

8. The cosmetic according to claim 7, wherein The cosmetic matrix includes at least one of a thickener, a moisturizer, an emulsifier, a preservative, a fragrance, and a pH adjuster.

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