Traditional Chinese medicine composition for preventing and treating fowl adenovirus and pharmaceutical preparation thereof

Abstract

The invention relates to a traditional Chinese medicine composition for preventing and treating fowl adenovirus and a pharmaceutical preparation thereof, and belongs to the technical field of traditional Chinese medicine preparations. The traditional Chinese medicine composition is prepared from, by weight, 23-37 parts of cortex erythrinae, 23-37 parts of herba artemisiae scopariae, 15-25 parts of radix isatidis, 5-15 parts of honeysuckle flowers, 5-15 parts of semen cuscutae, 5-11 parts of herba ecliptae, 5-11 parts of fructus aurantii and 3-9 parts of caulis polygoni multiflori. Pharmacodynamic experiments prove that the traditional Chinese medicine composition can remarkably improve symptom points; the content of chlorogenic acid in the prepared oral liquid is stable, and the oral liquid has relatively good chemical stability under an accelerated condition and is suitable for further popularization and application.

Description

Technical Field

[0001] The present invention belongs to the technical field of traditional Chinese medicine preparations, and relates to a traditional Chinese medicine composition for preventing and treating fowl adenovirus and its pharmaceutical preparation. Background Art

[0002] In recent years, Fowl Adenovirus FAdV serotype I has shown an explosive epidemic trend globally, causing huge economic losses to the poultry industry. FAdV serotype I can cause various diseases in poultry, such as inclusion body hepatitis (IBH), hydropericardium syndrome (HPS), and gizzard erosion (GE). The epidemic of FAdV serotype I in China can be divided into three stages: The first stage was from 1976 to 2007, and the main symptoms of diseased poultry were IBH, showing a sporadic state with little loss; the second stage was from 2007 to 2014, and the symptoms of diseased poultry were still mainly IBH, showing an endemic epidemic, with the infection rate of chicken flocks as high as 30% and the mortality rate of 15%; the third stage began to prevail widely in China in 2015, and the main symptoms were IBH and HPS, showing an outbreak situation. Summary of the Invention

[0003] In view of the defects of the prior art, the main object of the present invention is to provide a traditional Chinese medicine composition mainly containing Cortex Erythrinae, Herba Artemisiae Scopariae, Radix Isatidis, Flos Lonicerae Japonicae, Semen Cuscutae, Herba Ecliptae, Fructus Aurantii, and Caulis Polygoni Multiflori, and its pharmaceutical preparation. It solves the problem of fowl adenovirus infection and has good economic benefits. The dosage form prepared from the traditional Chinese medicine composition of the present invention can be powder, oral liquid, granule or other dosage forms, and all the dosage forms can achieve the same or similar technical effects.

[0004] Specifically, the present invention adopts the following technical solutions to achieve the above object: A traditional Chinese medicine composition for preventing and treating fowl adenovirus, the traditional Chinese medicine composition is made from the following raw materials in parts by weight: 23 - 37 parts of Cortex Erythrinae, 23 - 37 parts of Herba Artemisiae Scopariae, 15 - 25 parts of Radix Isatidis, 5 - 15 parts of Flos Lonicerae Japonicae, 5 - 15 parts of Semen Cuscutae, 5 - 11 parts of Herba Ecliptae, 5 - 11 parts of Fructus Aurantii, and 3 - 9 parts of Caulis Polygoni Multiflori.

[0005] Further preferably, the traditional Chinese medicine composition is made from the following raw materials in parts by weight: 37 parts of Cortex Erythrinae, 37 parts of Herba Artemisiae Scopariae, 25 parts of Radix Isatidis, 15 parts of Flos Lonicerae Japonicae, 15 parts of Semen Cuscutae, 11 parts of Herba Ecliptae, 11 parts of Fructus Aurantii, and 9 parts of Caulis Polygoni Multiflori.

[0006] More preferably, the traditional Chinese medicine composition is made from the following raw materials by weight: 30 parts of cortex erythrinae, 30 parts of herba artemisiae scopariae, 20 parts of radix isatidis, 10 parts of flos lonicerae, 10 parts of semen cuscutae, 8 parts of herba ecliptae, 8 parts of fructus aurantii, 6 parts of caulis polygonati multiflori.

[0007] More preferably, the traditional Chinese medicine composition is made from the following raw materials by weight: 23 parts of cortex erythrinae, 23 parts of herba artemisiae scopariae, 15 parts of radix isatidis, 5 parts of flos lonicerae, 5 parts of semen cuscutae, 5 parts of herba ecliptae, 5 parts of fructus aurantii, 3 parts of caulis polygonati multiflori.

[0008] A pharmaceutical preparation for preventing and treating avian adenovirus, which is composed of the traditional Chinese medicine composition of the present invention and pharmaceutically acceptable excipients.

[0009] Furthermore, the pharmaceutical preparation is selected from any one of powder, oral liquid or granule.

[0010] Furthermore, the pharmaceutical preparation is selected from oral liquid.

[0011] Furthermore, the preparation method of the oral liquid includes the following steps: (1) Add cortex erythrinae, herba artemisiae scopariae, radix isatidis, semen cuscutae and caulis polygonati multiflori to 8 - 15 times the mass of water, soak for 4 - 8 h; carry out steam reflux decoction for 1.5 - 2.5 h, filter to obtain filtrate and residue; add 4 - 8 times the mass of water to the residue, carry out steam reflux decoction for 0.5 - 1.5 h, filter, combine the two filtrates, and reserve the residue; (2) Crush and mix flos lonicerae, herba ecliptae and fructus aurantii, add them to the residue in step (1), decoct with water twice for 0.5 - 1.5 hours, combine the two decoctions, filter to obtain filtrate; (3) Combine the filtrate in step (2) with the decocted liquid after distillation in step (1), concentrate at 80 - 90 °C to a relative density of 1.02 - 1.05, cool to room temperature to obtain the traditional Chinese medicine oral liquid; (4) Add 0.1 - 0.3% of butylated hydroxyanisole based on the total weight to step (3), and finally add citric acid to adjust the pH value to 4.5 - 5.5 to obtain the product.

[0012] Furthermore, the preparation method of the oral liquid includes the following steps: (1) Add cortex erythrinae, herba artemisiae scopariae, radix isatidis, semen cuscutae and caulis polygonati multiflori to 13 times the mass of water, soak for 6 h; carry out steam reflux decoction for 2 h, filter to obtain filtrate and residue; add 6 times the mass of water to the residue, carry out steam reflux decoction for 1 h, filter, combine the two filtrates, and reserve the residue; (2) Crush and mix flos lonicerae, herba ecliptae and fructus aurantii, add them to the residue in step (1), decoct with water twice for 1 hour, combine the two decoctions, filter to obtain filtrate; (3) The filtrate from step (2) is combined with the medicinal liquid after distillation in step (1), concentrated to a relative density of 1.03 at 80 - 90 °C, and cooled to room temperature to obtain the traditional Chinese medicine oral liquid; (4) Add 0.2% of butylated hydroxyanisole based on the total weight to step (3), and finally add citric acid to adjust the pH value to 5.0 to obtain the product.

[0013] Compared with the prior art, the present invention has the following beneficial effects: 1) The traditional Chinese medicine composition of the present invention has all the monarch, minister, assistant, and envoy herbs, hits the mechanism accurately, and has definite curative effects. Erythrina bark is used for clearing heat and detoxifying, and can treat various diseases caused by heat toxin accumulation; Artemisia capillaris can clear heat and detoxify, promote diuresis and remove jaundice. The two are used as the monarch herbs together. Honeysuckle is a substance for clearing heat and detoxifying, and plays the role of clearing heat and detoxifying together with Isatis root. These two herbs complement the monarch herbs and can enhance the overall effect of clearing heat and detoxifying. Cuscuta chinensis, as the minister herb, has the effect of enhancing the body's resistance and avoiding deficiency caused by excessive clearing of heat and detoxification. The addition of Cuscuta chinensis plays the role of regulating the properties of the medicine, nourishing the liver and kidney, and avoiding the prescription from being overly cold or overly inclined to clearing heat, making the overall effect of the prescription more balanced. Assisted by Eclipta prostrata and Fructus Aurantii, it promotes the smooth flow of qi, and cooperates with the promoting blood circulation and regulating qi effect of Erythrina bark, can regulate qi and blood, and reduce the discomfort that may be brought about by excessive heat clearing. Finally, Polygonum multiflorum Thunb. is added to coordinate the effect of the prescription, enable the functions of other traditional Chinese medicines to be exerted, and guide other drugs to play a role in clearing heat and detoxifying.

[0014] 2) The pharmacodynamic experiment confirmed that from the weight detection in the first week, the traditional Chinese medicine composition of the present invention can significantly increase the weight of white - feather chickens and improve the syndrome score. There are significant differences between Examples 1 - 3 and Comparative Examples 1 - 4 (P < 0.01 or P < 0.0001).

[0015] 3) The preferred extraction and preparation process of the present invention ensures the efficient extraction of chlorogenic acid by precisely controlling extraction conditions such as temperature, time, and concentration. In the prepared oral liquid, the content of chlorogenic acid is above 45 mg / g. After the accelerated stability test, the content of chlorogenic acid remains stable without significant changes. The inventor believes that it may be that butylated hydroxyanisole forms a relatively stable system, thus protecting chlorogenic acid from being degraded. BRIEF DESCRIPTION OF THE DRAWINGS

[0016] Figure 1 : Experiment on the effect of the traditional Chinese medicine composition with different example components on the weight of white - feather chickens at the initial stage, the 1st week, and the 2nd week.

[0017] Figure 2 : Experiment on the effect of the traditional Chinese medicine composition with different example components on the symptom score of white - feather chickens.

[0018] Figure 3:Contents of chlorogenic acid in Example 1, Comparative Example 5, Comparative Example 6, Comparative Example 7, and Comparative Example 8 at the 0th month, 1st month, 2nd month, 3rd month, and 6th month after accelerated testing. Detailed implementation mode

[0019] The present invention will be further illustrated below in conjunction with specific embodiments. It should be understood that these embodiments are only used to illustrate the present invention and not to limit the scope of the present invention. After reading the present invention, various equivalent modifications made by those skilled in the art fall within the scope protected by the claims of this application.

[0020] Example 1: A pharmaceutical preparation for preventing and treating avian adenovirus, with the content and preparation process as follows: 30 g of pittosporum bark, 30 g of virgate wormwood, 20 g of isatis root, 10 g of honeysuckle flower, 10 g of dodder seed, 8 g of eclipta prostrata, 8 g of bitter orange, 6 g of polygonum multiflorum stem.

[0021] (1) Add pittosporum bark, virgate wormwood, isatis root, dodder seed, and polygonum multiflorum stem to 13 times the mass of water, soak for 6 h; steam reflux and decoct for 2 h, filter to obtain filtrate and filter residue; add 6 times the mass of water to the filter residue, steam reflux and decoct for 1 h, filter, combine the two filtrates, and reserve the filter residue; (2) Crush and mix honeysuckle flower, eclipta prostrata, and bitter orange, add them to the filter residue in step (1), decoct with water twice for 1 hour, combine the two decoctions, filter to obtain filtrate; (3) Combine the filtrate in step (2) with the decocted liquid after distillation in step (1), concentrate to a relative density of 1.03 at 80 - 90 °C, cool to room temperature to obtain a traditional Chinese medicine oral liquid; (4) Add 0.2% of butylated hydroxyanisole based on the total weight to step (3), and finally add citric acid to adjust the pH value to 5.0 to obtain the product.

[0022] Example 2: A pharmaceutical preparation for preventing and treating avian adenovirus, with the content and preparation process as follows: 23 g of pittosporum bark, 23 g of virgate wormwood, 15 g of isatis root, 5 g of honeysuckle flower, 5 g of dodder seed, 5 g of eclipta prostrata, 5 g of bitter orange, 3 g of polygonum multiflorum stem.

[0023] (1) Add pittosporum bark, virgate wormwood, isatis root, dodder seed, and polygonum multiflorum stem to 8 times the mass of water, soak for 4 h; steam reflux and decoct for 1.5 h, filter to obtain filtrate and filter residue; add 4 times the mass of water to the filter residue, steam reflux and decoct for 0.5 h, filter, combine the two filtrates, and reserve the filter residue; (2) Crush and mix honeysuckle flower, eclipta prostrata, and bitter orange, add them to the filter residue in step (1), decoct with water twice for 0.5 hour, combine the two decoctions, filter to obtain filtrate; (3) The filtrate from step (2) is combined with the medicinal liquid after distillation in step (1), concentrated to a relative density of 1.02 at 80 °C, cooled to room temperature, to obtain the traditional Chinese medicine oral liquid; (4) Add 0.1% of butylated hydroxyanisole based on the total weight to step (3), and finally add citric acid to adjust the pH value to 4.5 to obtain the product.

[0024] Example 3: A pharmaceutical preparation for preventing and treating avian adenovirus, with the content and preparation process as follows: Cortex Erythrinae 37g, Herba Artemisiae Scopariae 37g, Radix Isatidis 25g, Flos Lonicerae 15g, Semen Cuscutae 15g, Herba Ecliptae 11g, Fructus Aurantii 11g, Caulis Polygoni Multiflori 9g.

[0025] (1) Add Cortex Erythrinae, Herba Artemisiae Scopariae, Radix Isatidis, Semen Cuscutae, and Caulis Polygoni Multiflori to 15 times the mass of water, soak for 8 h; steam reflux and decoct for 2.5 h, filter to obtain the filtrate and residue; add 8 times the mass of water to the residue, steam reflux and decoct for 1.5 h, filter, combine the two filtrates, and reserve the residue; (2) Pulverize and mix Flos Lonicerae, Herba Ecliptae, and Fructus Aurantii, add them to the residue from step (1), decoct with water twice for 1.5 hours, combine the two decoctions, filter to obtain the filtrate; (3) The filtrate from step (2) is combined with the medicinal liquid after distillation in step (1), concentrated to a relative density of 1.05 at 80 - 90 °C, cooled to room temperature, to obtain the traditional Chinese medicine oral liquid; (4) Add 0.1 - 0.3% of butylated hydroxyanisole based on the total weight to step (3), and finally add citric acid to adjust the pH value to 5.5 to obtain the product.

[0026] Comparative Example 1: A pharmaceutical preparation for preventing and treating avian adenovirus, with the content and preparation process as follows: Herba Artemisiae Scopariae 30g, Radix Isatidis 20g, Flos Lonicerae 10g, Semen Cuscutae 10g, Herba Ecliptae 8g, Fructus Aurantii 8g, Caulis Polygoni Multiflori 6g.

[0027] (1) Add Herba Artemisiae Scopariae, Radix Isatidis, Semen Cuscutae, and Caulis Polygoni Multiflori to 13 times the mass of water, soak for 6 h; steam reflux and decoct for 2 h, filter to obtain the filtrate and residue; add 6 times the mass of water to the residue, steam reflux and decoct for 1 h, filter, combine the two filtrates, and reserve the residue; (2) Pulverize and mix Flos Lonicerae, Herba Ecliptae, and Fructus Aurantii, add them to the residue from step (1), decoct with water twice for 1 hour, combine the two decoctions, filter to obtain the filtrate; (3) The filtrate from step (2) is combined with the medicinal liquid after distillation in step (1), concentrated to a relative density of 1.03 at 80 - 90 °C, cooled to room temperature, to obtain the traditional Chinese medicine oral liquid; (4) Add butylated hydroxyanisole with a total weight of 0.2% to step (3), and finally add citric acid to adjust the pH value to 5.0 to obtain the product.

[0028] Comparative Example 2: A pharmaceutical preparation for preventing and treating avian adenovirus, with the content and preparation process as follows: 15 g of cortex erythrinae, 45 g of artemisia capillaris, 20 g of isatis root, 10 g of honeysuckle flower, 10 g of cuscuta chinensis, 8 g of eclipta prostrata, 8 g of fructus aurantii, 6 g of polygonum multiflorum thunb.

[0029] (1) Add cortex erythrinae, artemisia capillaris, isatis root, cuscuta chinensis, and polygonum multiflorum thunb. to water with a mass 13 times that of the raw materials, and soak for 6 h; reflux and decoct with steam for 2 h, filter to obtain a filtrate and residues; add water with a mass 6 times that of the residues, reflux and decoct with steam for 1 h, filter, combine the two filtrates, and reserve the residues; (2) Pulverize and mix honeysuckle flower, eclipta prostrata, and fructus aurantii, add them to the residues from step (1), decoct with water twice for 1 hour each time, combine the two decoctions, filter to obtain a filtrate; (3) Combine the filtrate from step (2) with the decocted liquid after distillation in step (1), concentrate at 80 - 90 °C to a relative density of 1.03, and cool to room temperature to obtain a traditional Chinese medicine oral liquid; (4) Add butylated hydroxyanisole with a total weight of 0.2% to step (3), and finally add citric acid to adjust the pH value to 5.0 to obtain the product.

[0030] Comparative Example 3: A pharmaceutical preparation for preventing and treating avian adenovirus, with the content and preparation process as follows: 90 g of salvia miltiorrhiza, 90 g of agrimonia pilosa, 30 g of euphorbia humifusa willd, 30 g of poria cocos, 30 g of halloysitum rubrum, 30 g of licorice root.

[0031] (1) Pulverize and mix each raw material component of the above - mentioned pharmaceutical preparation for preventing and treating avian group I adenovirus (90 g each of salvia miltiorrhiza and agrimonia pilosa, 30 g each of euphorbia humifusa willd, poria cocos, halloysitum rubrum, and licorice root), add them to an extraction tank, inject clear water, soak and rinse to remove impurities and dust; add clear water with a mass 8 times that of the raw material components to the rinsed raw material components, soak for 8 h; then reflux and decoct with steam for 3 h, filter to obtain a filtrate and residues; add clear water with a mass 4 times that of the residues, reflux and decoct with steam for 2 h, filter, and combine the two filtrates; (2) Concentrate the filtrate obtained in step (1) to a specific gravity of 1.15, and adjust the pH value to 5.0 with 1% hydrochloric acid by mass to obtain a pharmaceutical preparation for preventing and treating avian group I adenovirus; (3) Add sodium benzoate for preservation to the pharmaceutical preparation for preventing and treating avian group I adenovirus obtained in step (2) to obtain a finished - product pharmaceutical preparation for preventing and treating avian group I adenovirus, wherein the mass fraction of sodium benzoate in the finished - product pharmaceutical preparation for preventing and treating avian group I adenovirus is 0.3%.

[0032] Comparative Example 4: A pharmaceutical preparation for preventing and treating avian adenovirus, with the content and preparation process as follows: 15 g of Isatis root, 10 g of White peony root, 20 g of Capillary artemisia, 10 g of Chinese gentian, 7.5 g of Codonopsis pilosula, 7.5 g of Poria cocos, 10 g of Scutellaria baicalensis, 10 g of Sophora flavescens, 10 g of Licorice root, 15 fen of Asiatic plantain herb, 20 g of Lysimachia christinae Hance.

[0033] The above raw materials are crushed and passed through a 300-mesh sieve to obtain a pharmaceutical preparation, which is decocted twice with water for 1 hour each time. The two decoctions are combined, filtered to obtain a filtrate, concentrated to a relative density of 1.03 at 80 - 90 °C, cooled to room temperature to obtain a traditional Chinese medicine oral liquid; 0.2% of butylated hydroxyanisole based on the total weight is added, and finally citric acid is added to adjust the pH value to 5.0 to obtain the product.

[0034] Comparative Example 5: A pharmaceutical preparation for preventing and treating avian adenovirus, with the content and preparation process as follows: 30 g of Chinese photinia bark, 30 g of Capillary artemisia, 20 g of Isatis root, 10 g of Honeysuckle flower, 10 g of Chinese dodder, 8 g of Eclipta prostrata, 8 g of Immature bitter orange, 6 g of Nightshade vine.

[0035] Chinese photinia bark, Capillary artemisia, Isatis root, Honeysuckle flower, Chinese dodder, Eclipta prostrata, Immature bitter orange, and Nightshade vine are added to 13 times the mass of water and soaked for 6 h; refluxed and decocted with steam for 2 h, filtered to obtain a filtrate and filter residue; 6 times the mass of water is added to the filter residue, refluxed and decocted with steam for 1 h, filtered, and the two filtrates are combined, concentrated to a relative density of 1.03 at 80 - 90 °C, cooled to room temperature to obtain a traditional Chinese medicine oral liquid; 0.2% of butylated hydroxyanisole based on the total weight is added, and finally citric acid is added to adjust the pH value to 5.0 to obtain the product.

[0036] Comparative Example 6: A pharmaceutical preparation for preventing and treating avian adenovirus, with the content and preparation process as follows: 30 g of Chinese photinia bark, 30 g of Capillary artemisia, 20 g of Isatis root, 10 g of Honeysuckle flower, 10 g of Chinese dodder, 8 g of Eclipta prostrata, 8 g of Immature bitter orange, 6 g of Nightshade vine.

[0037] Chinese photinia bark, Capillary artemisia, Isatis root, Chinese dodder, Nightshade vine, Honeysuckle flower, Eclipta prostrata, and Immature bitter orange are crushed and mixed, then refluxed with ethanol for 3 hours, concentrated to a relative density of 1.03 at 40 - 45 °C, cooled to room temperature to obtain a traditional Chinese medicine oral liquid; 0.2% of butylated hydroxyanisole based on the total weight is added, and finally citric acid is added to adjust the pH value to 5.0 to obtain the product.

[0038] Comparative Example 7: A pharmaceutical preparation for preventing and treating avian adenovirus, with the content and preparation process as follows: 30 g of Chinese photinia bark, 30 g of Capillary artemisia, 20 g of Isatis root, 10 g of Honeysuckle flower, 10 g of Chinese dodder, 8 g of Eclipta prostrata, 8 g of Immature bitter orange, 6 g of Nightshade vine.

[0039] (1) Add cortex erythrinae, herba artemisiae scopariae, radix isatidis, semen cuscutae, and caulis polygonati multiflori to 13 times the mass of water, soak for 6 h; reflux and decoct with steam for 2 h, filter to obtain filtrate and residue; add 6 times the mass of water to the residue, reflux and decoct with steam for 1 h, filter, combine the two filtrates, and reserve the residue. (2) Pulverize and mix flos lonicerae, eclipta prostrata, and fructus aurantii immaturus, add them to the residue from step (1), decoct with water twice for 1 h, combine the two decoctions, filter to obtain filtrate. (3) Combine the filtrate from step (2) with the medicinal liquid after distillation in step (1), concentrate at 80 - 90 °C to a relative density of 1.03, cool to room temperature to obtain the traditional Chinese medicine oral liquid. (4) Add sodium benzoate at 0.2% of the total weight to step (3), and finally add citric acid to adjust the pH value to 5.0 to obtain the product.

[0040] Comparative Example 8: A pharmaceutical preparation for preventing and treating avian adenovirus, with the content and preparation process as follows: 30 g of cortex erythrinae, 30 g of herba artemisiae scopariae, 20 g of radix isatidis, 10 g of flos lonicerae, 10 g of semen cuscutae, 8 g of eclipta prostrata, 8 g of fructus aurantii immaturus, 6 g of caulis polygonati multiflori.

[0041] (1) Add cortex erythrinae, herba artemisiae scopariae, radix isatidis, semen cuscutae, and caulis polygonati multiflori to 13 times the mass of water, soak for 6 h; reflux and decoct with steam for 2 h, filter to obtain filtrate and residue; add 6 times the mass of water to the residue, reflux and decoct with steam for 1 h, filter, combine the two filtrates, and reserve the residue. (2) Pulverize and mix flos lonicerae, eclipta prostrata, and fructus aurantii immaturus, add them to the residue from step (1), decoct with water twice for 1 h, combine the two decoctions, filter to obtain filtrate. (3) Combine the filtrate from step (2) with the medicinal liquid after distillation in step (1), concentrate at 80 - 90 °C to a relative density of 1.03, cool to room temperature to obtain the traditional Chinese medicine oral liquid. (4) Add butylated hydroxyanisole at 0.05% of the total weight to step (3), and finally add hydrochloric acid to adjust the pH value to 5.0 to obtain the product.

[0042] I. Pharmacodynamic experiments of the drug of the present invention 1.1 Chicken model infected with type I adenovirus Put 5 white - feather broiler chickens diagnosed with type I adenovirus into a group of 150 three - week - old white - feather broiler experimental chickens for mixed breeding, conduct an infection experiment, observe the changes in the chicken group after 5 days, and select the experimental chickens according to the scores in Table 1: Table 1 Score classification and symptom statistics Note: If the total score is below 3, it is diagnosed as a sick chicken infected with type I adenovirus.

[0043] 1.2 Grouping and Administration Take the type I adenovirus model chickens prepared in 1.1, and then randomly divide them into 8 groups, namely the Example 1 group, the Example 2 group, the Example 3 group, the Comparative Example 1 group, the Comparative Example 2 group, the Comparative Example 3 group, the Comparative Example 4 group, and the Model group, with 10 chickens in each group. Administer the oral liquid prepared in the corresponding example to each chicken, gavage 0.2 mL per chicken, twice a day, for 7 consecutive days. Another 10 healthy chicken flocks (white - feather broilers) are selected as the blank group control. The blank group and the model group are both gavaged with the same dose of 0.9% physiological saline.

[0044] 1.3 Detection 1.3.1 Detection of initial body weight, body weight in the 1st week, and body weight in the 2nd week Body weight measurement is an important indicator for evaluating the treatment effect and the health status of animals. Body weight reflects the health status. At the end of the 1st week and the 2nd week of treatment, the individuals in each group are weighed.

[0045] 1.3.2 Improvement of integral After modeling, there is no significant difference in the integral among the experimental groups, and the integral distribution is between 0 - 3 points. After 1 - week treatment, the integral status is finally calculated.

[0046] 1.4 Statistical Analysis Use Graphad Prism 9.0 for data statistics. The obtained data is expressed as mean ± standard deviation ( ±S). Perform a normal distribution test and a homogeneity of variance test on the data of each group. Meeting the requirements of Pearson's test, one - way ANOVA is used for comparison between groups. P < 0.05 is considered statistically significant.

[0047] 1.5 Result Analysis 1.5.1 Analysis of body weight in the 1st week and body weight in the 2nd week. See Table 2 and Figure 1 Table 2 Analysis of body weight in the 1st week and body weight in the 2nd week of relevant examples Figure 1 It can be seen that: there are great differences in the weight of the pharmaceutical preparations of different examples. At the 0th week, there is no significant difference among the examples (P > 0.05). From the body weight detection in the 1st week, it can be seen that there is a significant difference between Examples 1 - 3 and Comparative Examples 1 - 4 (P < 0.05). The inventor believes that removing Pittosporum bark or changing the dosage, substitution, etc. will all affect the therapeutic effect of the whole composition. This difference is more obvious at the 2nd week, and the growth rate of white - feather chickens in Comparative Examples 1 - 4 is significantly reduced.

[0048] 1.5.2 Improvement of points, see Table 3 and Figure 2 Table 3 Related Examples Point Improvement Figure 2 It can be seen that at the end of the first week after treatment, the scores of each example group increased, indicating the therapeutic effect of the drug preparation on adenovirus. A comparison of Examples 1-3 and Comparative Examples 1-4 showed that the scores of Comparative Examples 1-4 were significantly lower than those of Examples 1-3, and this significant feature had a significant difference (P<0.0001).

[0049] 2. Determination of Chlorogenic Acid Content [Content Determination] Chlorogenic acid is determined according to high performance liquid chromatography (General Rule 0512).

[0050] Chromatographic conditions and system suitability test: octadecylsilane bonded silica gel was used as filler; acetonitrile-0.05% phosphoric acid solution (10:90) was used as mobile phase; and the detection wavelength was 327 nm.

[0051] Preparation of reference solution: Take an appropriate amount of chlorogenic acid reference substance, weigh it accurately, place it in a brown volumetric flask, and add 50% methanol to make a solution containing 40 μg per 1 mL.

[0052] Preparation of test solution: Take 0.3 mL of solution of Example 1, Comparative Example 5, Comparative Example 6, Comparative Example 7 and Comparative Example 8, place in a 50 mL brown volumetric flask, add 50% methanol to the scale, shake well, centrifuge, accurately measure 3 mL of supernatant, place in a 10 mL brown volumetric flask, add 50% methanol to the scale, shake well, and obtain.

[0053] Determination method: Accurately pipette 15 μL of reference solution and test solution respectively, inject into liquid chromatograph, and determine.

[0054] Accelerated test: Store at 40℃±2℃, 75%±5% relative humidity (RH), and test the chlorogenic acid content at 0, 1, 2, 3, and 6 months. Figure 3 And as shown in Table 4.

[0055] Table 4 Content of chlorogenic acid in relevant examples from Figure 3It can be seen that the chlorogenic acid content in the examples is above 45 mg / mL. In Comparative Examples 7-8, the chlorogenic acid content decreased significantly during storage. The inventor believes that it may be due to the reaction of different preservatives with chlorogenic acid in the oral liquid, resulting in a decrease in the activity of chlorogenic acid. It is also possible that the buffer system formed by the preservatives is not suitable for the storage environment of chlorogenic acid, leading to a decrease in chlorogenic acid. Comparative Examples 5-6 used different extraction processes and showed a lower chlorogenic acid content at the 0th month.

Claims

1. A traditional Chinese medicine composition for preventing and treating avian adenovirus, which is made from the following raw materials in parts by weight: 23 - 37 parts of pittosporum bark, 23 - 37 parts of virgate wormwood, 15 - 25 parts of isatis root, 5 - 15 parts of honeysuckle flower, 5 - 15 parts of dodder seed, 5 - 11 parts of eclipta prostrata, 5 - 11 parts of bitter orange, 3 - 9 parts of polygonum multiflorum thunb.

2. The traditional Chinese medicine composition for preventing and treating avian adenovirus according to claim 1, wherein The traditional Chinese medicine composition is made from the following raw materials in parts by weight: 30 parts of pittosporum bark, 30 parts of virgate wormwood, 20 parts of isatis root, 10 parts of honeysuckle flower, 10 parts of dodder seed, 8 parts of eclipta prostrata, 8 parts of bitter orange, 6 parts of polygonum multiflorum thunb.

3. The traditional Chinese medicine composition for preventing and treating avian adenovirus according to claim 1, wherein, The traditional Chinese medicine composition is made from the following raw materials in parts by weight: 23 parts of pittosporum bark, 23 parts of virgate wormwood, 15 parts of isatis root, 5 parts of honeysuckle flower, 5 parts of dodder seed, 5 parts of eclipta prostrata, 5 parts of bitter orange, 3 parts of polygonum multiflorum thunb.

4. The traditional Chinese medicine composition for preventing and treating fowl adenovirus according to claim 1, wherein, The traditional Chinese medicine composition is made from the following raw materials in parts by weight: 37 parts of pittosporum bark, 37 parts of virgate wormwood, 25 parts of isatis root, 15 parts of honeysuckle flower, 15 parts of dodder seed, 11 parts of eclipta prostrata, 11 parts of bitter orange, 9 parts of polygonum multiflorum thunb.

5. A pharmaceutical preparation for preventing and treating avian adenovirus, characterized in that, The pharmaceutical preparation is composed of the traditional Chinese medicine composition according to any one of claims 1 - 4 and pharmaceutically acceptable excipients.

6. The pharmaceutical preparation for preventing and treating avian adenovirus according to claim 5, characterized in that, The pharmaceutical preparation is selected from any one of powder, oral liquid or granule.

7. The pharmaceutical preparation for preventing and treating avian adenovirus according to claim 6, characterized in that, The pharmaceutical preparation is selected from oral liquid.

8. The pharmaceutical preparation for preventing and treating avian adenovirus according to claim 7, characterized in that, The preparation method of the oral liquid includes the following steps: (1) Add pittosporum bark, virgate wormwood, isatis root, dodder seed, polygonum multiflorum thunb into water with a mass 8 - 15 times, soak for 4 - 8 h; carry out steam reflux extraction for 1.5 - 2.5 h, filter to obtain filtrate and residue; add water with a mass 4 - 8 times into the residue, carry out steam reflux extraction for 0.5 - 1.5 h, filter, combine the two filtrates, and reserve the residue; (2) Crush and mix honeysuckle flower, eclipta prostrata, bitter orange and add them into the residue obtained in step (1), decoct with water twice for 0.5 - 1.5 hours, combine the two decoctions, filter to obtain filtrate; (3) Combine the filtrate obtained in step (2) with the decoction after distillation in step (1), concentrate at 80 - 90 °C to a relative density of 1.02 - 1.05, cool to room temperature to obtain the traditional Chinese medicine oral liquid; (4) Add 0.1 - 0.3% of butylated hydroxyanisole based on the total weight into step (3), and finally add citric acid to adjust the pH value to 4.5 - 5.5 to obtain the product.

9. The pharmaceutical preparation for preventing and treating avian adenovirus according to claim 8, wherein, The preparation method of the oral liquid includes the following steps: (1) Add pittosporum bark, virgate wormwood, isatis root, dodder seed, polygonum multiflorum thunb into water with a mass 13 times, soak for 6 h; carry out steam reflux extraction for 2 h, filter to obtain filtrate and residue; add water with a mass 6 times into the residue, carry out steam reflux extraction for 1 h, filter, combine the two filtrates, and reserve the residue; (2) Crush and mix honeysuckle flower, eclipta prostrata, bitter orange and add them into the residue obtained in step (1), decoct with water twice for 1 hour, combine the two decoctions, filter to obtain filtrate; (3) Combine the filtrate obtained in step (2) with the decoction after distillation in step (1), concentrate at 80 - 90 °C to a relative density of 1.03, cool to room temperature to obtain the traditional Chinese medicine oral liquid; (4) Add 0.2% of butylated hydroxyanisole based on the total weight into step (3), and finally add citric acid to adjust the pH value to 5.0 to obtain the product.

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