Traditional Chinese medicine feed additive for preventing and treating piglet diarrhea and application thereof

Abstract

The invention relates to a feed additive, and aims to provide a traditional Chinese medicine feed additive for preventing and treating piglet diarrhea and application of the traditional Chinese medicine feed additive. The product takes Chinese pulsatilla root as a monarch drug, amur corktree bark as a ministerial drug, bighead atractylodes rhizome, white paeony root, costus root and tangerine peel as adjuvant drugs, and liquorice as a conductant drug; the traditional Chinese medicine composition is prepared from the following raw materials in parts by weight: 190 to 210 parts of radix pulsatillae, 190 to 210 parts of cortex phellodendri, 140 to 160 parts of rhizoma atractylodis macrocephalae, 110 to 130 parts of radix paeoniae alba, 110 to 130 parts of radix aucklandiae, 110 to 130 parts of pericarpium citri reticulatae and 80 to 100 parts of liquorice root. By innovatively adopting a compatibility strategy of simultaneously treating liver-spleen-intestine, the Chinese pulsatilla root and the amur corktree bark are used for treating dampness and heat in intestinal tracts, the bighead atractylodes rhizome and the liquorice root are used for invigorating spleen and replenishing qi, and the white paeony root is used for softening liver and relieving The purpose of treating the intestinal diseases of the piglets is achieved by adopting a multi-angle and multi-level comprehensive means, and the limitation of purely treating the intestinal diseases is broken through. The product can also improve the structure of intestinal flora of the piglets, reduce the abundance of harmful bacteria, improve the health of the intestinal tracts, enhance the immunity of the organism and effectively reduce the probability of diarrhea of the piglets.

Description

Technical Field

[0001] The invention relates to the field of feed additives, and in particular to a traditional Chinese medicine feed additive for preventing and treating piglet diarrhea and application thereof. Background Art

[0002] Intestinal health issues are an important factor restricting the high-quality and efficient development of pig farming. Due to the incomplete development of digestive organs, newborn piglets are very prone to stress reactions or infections, causing health problems such as diarrhea and slow growth, increasing the mortality rate, and thus affecting the overall economic benefits of the pig farming industry. As the only growth-promoting drug feed additive that can be produced and used, traditional Chinese medicine has the advantages of low residue, low toxicity and side effects, and no significant drug resistance, and has important application potential.

[0003] However, traditional Chinese medicine treatment ideas usually focus on the single dimension of dampness and heat in the intestines. For example, Bai Tou Weng San is mainly used to clear dampness and heat in the intestines, so it is used to treat diarrhea. However, the pathogenesis of piglet diarrhea is relatively complex. In addition to the dampness and heat factors caused by intestinal infection, weaning stress, feed changes and other factors often lead to poor liver qi and liver dysfunction in piglets, which in turn affects the transportation and transformation function of the spleen and stomach. The spleen and stomach functions of piglets are not yet sound, the spleen fails to function properly, water and grain cannot be transported normally, endogenous dampness and external dampness and heat are mixed together, aggravating diarrhea symptoms. The existing Chinese medicine compatibility schemes fail to fully consider the synergistic relationship between the liver, spleen, and intestines, and are deficient in overall conditioning. It is difficult to fundamentally improve the body state of piglets, resulting in diarrhea problems that are easy to recur, seriously affecting the growth and development of piglets and the economic benefits of breeding.

[0004] Therefore, it is of great significance to explore a green, healthy and efficient Chinese herbal feed additive to prevent piglet diarrhea, regulate intestinal health and reduce mortality rate. Summary of the invention

[0005] The technical problem to be solved by the present invention is to overcome the deficiencies of the prior art and provide a Chinese medicinal feed additive for preventing and treating piglet diarrhea and application thereof.

[0006] To solve the technical problem, the solution of the present invention is:

[0007] Provided is a traditional Chinese medicine feed additive for preventing and treating piglet diarrhea. The traditional Chinese medicine feed additive comprises Pulsatilla striata as a main drug, Phellodendron chinense as a secondary drug, Atractylodes macrocephala, White peony root, Costus root and Tangerine peel as adjuvant drugs, and Licorice root as a guiding drug. The traditional Chinese medicine feed additive comprises the following raw materials in parts by weight: 190-210 parts of Pulsatilla striata, 190-210 parts of Phellodendron chinense, 140-160 parts of Atractylodes macrocephala, 110-130 parts of White peony root, 110-130 parts of Costus root, 110-130 parts of Tangerine peel and 80-100 parts of Licorice root.

[0008] As a preferred embodiment of the present invention, it is specifically composed of the following raw materials in parts by weight: 200 parts of Pulsatilla chinensis, 200 parts of Phellodendron amurense, 150 parts of Atractylodes macrocephala, 120 parts of Paeonia lactiflora, 120 parts of Aucklandia lappa, 120 parts of Citrus reticulata Blanco, and 90 parts of Glycyrrhiza uralensis Fisch.

[0009] As a preferred embodiment of the present invention, the dosage form is a powder, and the particle size range is 80 - 120 mesh.

[0010] The present invention further provides a preparation method of the aforementioned traditional Chinese medicine feed additive. After weighing each raw material according to the said parts by weight, drying treatment is carried out; after pulverization, it is sieved through a 80 - 120 mesh sieve, and then mixed evenly to obtain the feed additive product.

[0011] The present invention also provides the application of the aforementioned traditional Chinese medicine feed additive in the preparation of feed for preventing and treating piglet diarrhea.

[0012] The present invention also provides the application of the aforementioned traditional Chinese medicine feed additive in the preparation of feed for improving the intestinal microbial flora structure of piglets.

[0013] The present invention further provides feed for preventing and treating piglet diarrhea, which contains the aforementioned traditional Chinese medicine feed additive, and the mass percentage of the traditional Chinese medicine feed additive in the feed is 0.5% - 1%.

[0014] The present invention further provides feed for improving the intestinal microbial flora structure of piglets, which contains the aforementioned traditional Chinese medicine feed additive, and the mass percentage of the traditional Chinese medicine feed additive in the feed is 0.5% - 1%.

[0015] As a preferred embodiment of the present invention, in addition to the traditional Chinese medicine feed additive, it further includes a basal diet suitable for piglets; the components of the basal diet include corn, soybean meal, and fish meal.

[0016] The present invention further provides a method for using the aforementioned feed, including: taking an appropriate amount of feed and drinking water, stirring to make the traditional Chinese medicine feed additive and the basal diet evenly mixed to obtain the feeding material for piglets to eat.

[0017] Description of the invention principle:

[0018] The Chinese medicinal feed additive of the present invention achieves synchronous conditioning of the liver, spleen and intestines of piglets through unique and reasonable compatibility, and effectively treats diarrhea in piglets caused by liver and spleen imbalance and damp-heat accumulation in the colon. Specifically, it includes: using Pulsatilla striata as the main medicine, which is bitter and cold, good at clearing heat and toxicity in the large intestine, and has a significant therapeutic effect on diarrhea caused by heat and toxicity accumulation in the colon in weaned piglets. Phellodendron chinense is bitter and cold, clears heat and dries dampness, and helps Pulsatilla striata to clear dampness and heat in the large intestine, and is a ministerial medicine. Atractylodes macrocephala, white peony root, costus root and dried orange peel are adjuvants, among which Atractylodes macrocephala strengthens the spleen and dries dampness, can improve the transportation and transformation function of the spleen and stomach of piglets, white peony root nourishes blood and restrains yin, relieves urgency and relieves pain, can relieve intestinal spasms, and relieves discomfort in piglets; costus root promotes qi and relieves pain, strengthens the spleen and digests food, and dried orange peel regulates qi and strengthens the spleen, dries dampness and resolves phlegm, and the two assist Atractylodes macrocephala and white peony root in regulating the spleen and stomach qi, so that it is replenished without stagnation, and further plays the role of adjuvant. Licorice harmonizes various medicines and serves as a guiding medicine. It also has the ability to clear away heat, detoxify, relieve pain and stop pain.

[0019] The traditional Chinese medicine feed additive of the present invention can be mixed with a basic diet suitable for piglets to prepare feed, and the feed can reduce the diarrhea rate of piglets, improve the immunity of weaned piglets, and improve the structure of intestinal microbial flora.

[0020] Compared with the prior art, the present invention has the following beneficial effects:

[0021] 1. The applicant has found through long-term research that diarrhea in piglets is often caused by multiple factors such as intestinal infection (such as Escherichia coli, etc. causing damp heat), stress (affecting the liver's drainage), and incomplete spleen and stomach function (weak spleen). The present invention adopts an innovative liver-spleen-intestine treatment strategy, and provides a Chinese medicine composition composed of Pulsatilla striata, Phellodendron chinense, Atractylodes macrocephala, White Peony Root, Aucklandia lappa, Tangerine Peel, and Licorice; among them, Pulsatilla striata and Phellodendron chinense target intestinal damp heat, Atractylodes macrocephala and Licorice invigorate the spleen and replenish qi, and White Peony Root softens the liver and relieves urgency; a comprehensive approach with multiple angles and levels is adopted to achieve the purpose of treating intestinal diseases in piglets, breaking through the limitation of simply treating intestinal diseases.

[0022] 2. The Chinese medicinal feed additive of the present invention can improve the structure of the intestinal flora of piglets, reduce the abundance of harmful bacteria, improve intestinal health, and enhance the body's immunity, thereby effectively reducing the probability of diarrhea in weaned piglets. BRIEF DESCRIPTION OF THE DRAWINGS

[0023] Figure 1 This is the Venn diagram of cecal microbial OTUs in the validation experiment.

[0024] Figure 2 To verify the influence of Beta diversity of cecal microbial community in the experiment; (A) is PCoA analysis, and (B) is Anosim analysis.

[0025] Figure 3Analysis of cecal microbial composition in the verification test; (A) shows the top 30 species in terms of relative abundance at the phylum level and the analysis, and (B) shows the top 30 species in terms of relative abundance at the genus level and the analysis.

[0026] Figure 4 LEfSe species difference analysis (LDA > 3, P < 0.05) in the verification test. Detailed implementation manners

[0027] The present invention will be further described below in conjunction with the detailed implementation manners. The experimental methods in the examples are all conventional methods unless otherwise specified. The test materials used in the following examples are all commercially available through conventional channels unless otherwise specified.

[0028] The first part: The technical solution and specific examples of the present invention

[0029] 1. The traditional Chinese medicine feed additive for preventing and treating diarrhea in piglets provided by the present invention is composed of the following raw materials in parts by weight: 190 - 210 parts of Pulsatilla chinensis, 190 - 210 parts of Phellodendron amurense, 140 - 160 parts of Atractylodes macrocephala, 110 - 130 parts of Paeonia lactiflora, 110 - 130 parts of Aucklandia lappa, 110 - 130 parts of Citrus reticulata Blanco, and 80 - 100 parts of Glycyrrhiza uralensis Fisch. The most preferred compatibility ratio is: 200 parts of Pulsatilla chinensis, 200 parts of Phellodendron amurense, 150 parts of Atractylodes macrocephala, 120 parts of Paeonia lactiflora, 120 parts of Aucklandia lappa, 120 parts of Citrus reticulata Blanco, and 90 parts of Glycyrrhiza uralensis Fisch.

[0030] The preparation method of the traditional Chinese medicine feed additive includes: weighing each raw material according to the above-mentioned parts by weight, then performing a drying treatment; after pulverizing, passing through an 80 - 120 mesh sieve, and mixing evenly to obtain the feed additive product, and its dosage form is powder.

[0031] The traditional Chinese medicine feed additive of the present invention can be applied to the preparation of feeds for preventing and treating diarrhea in piglets and / or improving the intestinal microbial flora structure of piglets. Specifically, the traditional Chinese medicine feed additive is added to the basal diet suitable for piglets, and the mass percentage of the traditional Chinese medicine feed additive in the feed is 0.5% - 1%. In this way, feeds for preventing and treating diarrhea in piglets and / or improving the intestinal microbial flora structure of piglets are prepared. The usage method of the feed includes: taking appropriate amounts of feed and drinking water, stirring to mix the traditional Chinese medicine feed additive and the basal diet evenly to obtain the feeding material for piglets to eat.

[0032] The basal diet suitable for piglets can be a commercially available product, and its components usually include nutritional components such as corn, soybean meal, and fish meal; the present invention does not make specific limitations on the formula and content of the basal diet.

[0033] Example 1

[0034] Preparation of traditional Chinese medicine feed additive: 200 parts of Pulsatilla chinensis, 200 parts of Phellodendron amurense, 150 parts of Atractylodes macrocephala, 120 parts of Paeonia lactiflora, 120 parts of Aucklandia lappa, 120 parts of Citrus reticulata Blanco, 90 parts of Glycyrrhiza uralensis Fisch. After weighing each raw material according to the said weight parts, carry out drying treatment; after pulverization, sieve through a 80-mesh sieve, and mix evenly to obtain the feed additive product.

[0035] Preparation of feed: Add the traditional Chinese medicine feed additive to the basal diet, and the mass percentage of the traditional Chinese medicine feed additive in the feed is 0.5%.

[0036] Preparation of feeding material: Take appropriate amounts of feed and drinking water, stir to make the traditional Chinese medicine feed additive and the basal diet evenly mixed to obtain the feeding material for piglets.

[0037] Example 2

[0038] Preparation of traditional Chinese medicine feed additive: 190 parts of Pulsatilla chinensis, 210 parts of Phellodendron amurense, 160 parts of Atractylodes macrocephala, 110 parts of Paeonia lactiflora, 130 parts of Aucklandia lappa, 130 parts of Citrus reticulata Blanco, 80 parts of Glycyrrhiza uralensis Fisch. After weighing each raw material according to the said weight parts, carry out drying treatment; after pulverization, sieve through a 120-mesh sieve, and mix evenly to obtain the feed additive product.

[0039] Preparation of feed: Add the traditional Chinese medicine feed additive to the basal diet, and the mass percentage of the traditional Chinese medicine feed additive in the feed is 0.75%.

[0040] Preparation of feeding material: Take appropriate amounts of feed and drinking water, stir to make the traditional Chinese medicine feed additive and the basal diet evenly mixed to obtain the feeding material for piglets.

[0041] Example 3

[0042] Preparation of traditional Chinese medicine feed additive: 210 parts of Pulsatilla chinensis, 190 parts of Phellodendron amurense, 140 parts of Atractylodes macrocephala, 130 parts of Paeonia lactiflora, 110 parts of Aucklandia lappa, 110 parts of Citrus reticulata Blanco, 100 parts of Glycyrrhiza uralensis Fisch. After weighing each raw material according to the said weight parts, carry out drying treatment; after pulverization, sieve through a 100-mesh sieve, and mix evenly to obtain the feed additive product.

[0043] Preparation of feed: Add the traditional Chinese medicine feed additive to the basal diet, and the mass percentage of the traditional Chinese medicine feed additive in the feed is 1%.

[0044] Preparation of feeding material: Take appropriate amounts of feed and drinking water, stir to make the traditional Chinese medicine feed additive and the basal diet evenly mixed to obtain the feeding material for piglets.

[0045] The second part: Verification test and result analysis

[0046] Example 1: Effects of traditional Chinese medicine feed additive on diarrhea, immunity and intestinal flora structure of weaned piglets

[0047] 1 Materials and methods

[0048] 1.1 Experimental Design and Breeding Management

[0049] In the feed used for the experimental group, the specific components and addition amounts of the traditional Chinese medicine feed additive refer to Example 1.

[0050] Experimental Site: Zhejiang Jingdao Animal Husbandry Technology Co., Ltd. (Hangzhou, Zhejiang Province).

[0051] Experimental Animals: 110 weaned piglets (Duroc×Landrace×Yorkshire) at 32 days of age were randomly divided into 2 groups, with 4 or 6 litters in each group, and 9 - 13 pigs in each litter. The control group was fed a basal diet, and the experimental group was fed a basal diet supplemented with a traditional Chinese medicine feed additive (accounting for 0.5% of the total feed mass). The experimental period was 15 days. The daily management of the experimental site and the immunization measures for weaned piglets were carried out in accordance with the standard production procedures of the pig farm. During the experimental period, the temperature, ventilation, light and other conditions of each group were kept consistent, and free access to food and water was provided. After the experiment ended, blood samples were collected from the anterior vena cava, the pigs were slaughtered, and cecal content samples were collected for subsequent detection.

[0052] 1.2 Experimental Methods

[0053] (1) Growth Performance

[0054] After fasting for 12 hours, the experimental pigs were weighed on an empty stomach on the morning of the 1st day and the 15th day of the experiment respectively, and the initial weight and final weight of the piglets in each group were recorded to calculate the ADG (Average Daily Gain); the daily feed intake was recorded throughout the experimental period to calculate the ADFI (Average Daily Feed Intake); the feed to gain ratio (F / G) was calculated based on the ADFI and ADG.

[0055] (2) Diarrhea Rate

[0056] The feces of the piglets were checked regularly every morning and evening, and the diarrhea rate of the piglets was calculated according to the following formula:

[0057] Diarrhea Rate (%) = Number of Diarrheal Piglets in Each Group during the Experimental Period / Number of Piglets in Each Group at the Beginning of the Experiment × 100%.

[0058] (3) Determination of Classical Swine Fever Antibody and Porcine Reproductive and Respiratory Syndrome Antibody

[0059] The levels of classical swine fever antibody and porcine reproductive and respiratory syndrome antibody in serum samples were detected using the corresponding ELISA kits respectively, and the results were calculated and judged according to the following methods:

[0060] Calculation formula for classical swine fever antibody PI (Blocking Percentage): PI = (Mean OD of Negative Serum Control - OD Value of Sample) / Mean OD of Negative Serum Control × 100%, and a PI ≥ 40% is judged as positive;

[0061] Blue ear antibody S / P ratio calculation formula: S / P = (sample OD value - negative serum control OD mean) / (positive serum control OD mean - negative serum control OD mean), and a sample with S / P ≥ 0.4 is determined to be positive.

[0062] (4) Analysis of microbial composition in cecal contents

[0063] The cecal content samples were sent to Zhejiang Tianke High-Tech Development Co., Ltd. for 16S rDNA sequencing, and the specific analysis process includes the following operations.

[0064] Genomic DNA extraction and sample detection: Total bacterial DNA was extracted using the CTAB / SDS method, and the DNA concentration and integrity were detected by 1% agarose gel.

[0065] PCR amplification: In this experiment, primers for the V3-V4 hypervariable region of the 16S rDNA gene were used for PCR amplification.

[0066] Library construction and high-throughput sequencing: According to the characteristics of the amplified region, a small fragment library was constructed. After the constructed library passed the evaluation by Qubit@2.0 and Agilent 2100, PE250 high-throughput sequencing was performed using NovaSeq6000.

[0067] Sequencing data processing: According to the barcode and PCR amplification primer sequences, the data of each sample were split from the downloaded data. After removing the barcode and primer sequences, FLASH was used to splice the reads of each sample, and the obtained spliced sequences were the original Tags data (Raw Tags). After strict filtering, high-quality Tags data (Clean Tags) were obtained, and then chimera filtering was performed to obtain the final effective data (Effective Tags). DADA2 was used for noise reduction and deduplication to obtain ASV feature sequences and ASV abundance tables. Based on the annotation results of ASVs, Alpha diversity indices (observed_species, shannon, simpson, chao1, goods_coverage, Pielou_E, ACE) were calculated to analyze the microbial community diversity within the samples; Beta diversity was mainly evaluated by calculating four distances (weighted_unifrac, unweighted_unifrac, jaccard, bray_curtis) using six major analyses to assess the differences in microbial community structures between samples. Through LEfSe analysis, the bacterial communities with statistically significant differences between different groups were found.

[0068] 1.3 Data analysis

[0069] The experimental data were analyzed by one-way ANOVA using SPSS 26.0 software. The results were expressed as "mean ± standard error". A significant difference was defined as P < 0.05, and no significant difference was defined as P > 0.05.

[0070] 2 Experimental results

[0071] 2.1 Effects of traditional Chinese medicine feed additive on growth performance and diarrhea rate of weaned piglets

[0072] The effects of adding the traditional Chinese medicine composition to the feed on the growth performance and diarrhea rate of weaned piglets are shown in Table 1. As can be seen from Table 1, there was no significant difference in ADFI and ADG between the experimental group and the control group (P > 0.05); the diarrhea rate in the experimental group was significantly reduced, indicating that the traditional Chinese medicine feed additive of the present invention can improve its diarrhea condition.

[0073] Table 1 Effects of traditional Chinese medicine feed additive on growth performance and diarrhea rate of weaned piglets

[0074]

[0075] Note: Different lowercase letters in the same row of data indicate significant differences (P < 0.05), and no superscript or the same lowercase letter superscript indicates no significant difference (P > 0.05).

[0076] 2.2 Effects of traditional Chinese medicine feed additive on immunity of weaned piglets

[0077] The effects of the traditional Chinese medicine feed additive on the levels of classical swine fever antibody and porcine reproductive and respiratory syndrome antibody in the serum of weaned piglets are shown in Table 2. Compared with the control group, there was no significant difference in the level of classical swine fever antibody in the serum of weaned piglets in the experimental group (P > 0.05), but the level of porcine reproductive and respiratory syndrome antibody was significantly increased (P < 0.05). Judging by the antibody positive rate, both the classical swine fever antibody and porcine reproductive and respiratory syndrome antibody in the experimental group were significantly higher than those in the control group. The results indicate that the traditional Chinese medicine feed additive of the present invention can enhance the immune function of weaned piglets.

[0078] Table 2 Effects of traditional Chinese medicine feed additive on immunity of weaned piglets

[0079]

[0080] 2.3 Effects of traditional Chinese medicine feed additive on cecal microbial composition of weaned piglets

[0081] 2.3.1 OTU classification and diversity analysis

[0082] As Figure 1 shown, a total of 3510 different OTUs were classified from 6 samples. Among them, there were 1862 OTUs in the control group and 2037 OTUs in the experimental group, and 389 OTUs were common.

[0083] 2.3.2 Alpha diversity analysis

[0084] As shown in Table 3, Goods coverage reflects the coverage of low-abundance OTUs in the samples. Goodscoverage in both groups of samples was close to 1, indicating that the sequencing results covered most bacteria in the intestine; the ACE index and Chao1 index comprehensively reflect the community richness. The larger the value, the more species there are. Both indices showed an increasing trend in the experimental group, but there was no significant statistical difference (P>0.05).

[0085] Table 3 Effects of Chinese herbal medicine feed additives on the α-diversity of cecal fecal communities in weaned piglets

[0086]

[0087] 2.3.3 Beta diversity analysis

[0088] As Figure 2 shown in (A) below, principal coordinate analysis (PCoA) showed that the within-group distances of the two groups of cecal microbial samples were relatively close, the between-group distances were relatively far, and the samples were well aggregated. Through ANOSIM analysis as shown in (B) below, R>0, indicating that the within-group distance was less than the between-group distance, and the grouping was effective, but there was no statistical difference (P>0.05). Figure 2

[0089] 2.3.4 Bacterial community composition analysis

[0090] Based on the annotation results of OTUs, a total of 31 phyla, 83 classes, 184 orders, 325 families, and 672 genera were obtained for the two groups. The microbial community compositions of the two groups were statistically analyzed at each bacterial taxonomic level, and the bacterial communities with relatively high relative abundances were focused on. See Figure 3 . Figure 3 In (A) below, at the phylum level, the microbial communities of the two groups were mainly composed of Proteobacteria, Bacteroidota, Firmicutes, and Actinobacteriota, and the dominant bacterial community was Proteobacteria. At the same time, the abundance of Bacteroidota decreased in the experimental group, while the abundance of Firmicutes increased slightly. As shown in (B) below, at the genus level, the microbial communities of the two groups were mainly composed of Clostridium_sensu_stricto_1, Staphylococcus, Streptococcus, Lactobacillus, Corynebacterium, Weissella, etc. Figure 3

[0091] ​​2.3.5 LEfSe Species Difference Analysis

[0092] As Figure 4 shown, through LEfSe analysis (LDA > 3, P < 0.05), significantly enriched species in the cecal microbiota composition of two groups of weaned piglets were screened out. There were 17 genera significantly enriched in the intestinal tract of the control group, including Staphylococcus, Corynebacterium, Weissella, Staphylococcaceae_unclassified, Escherichia-Shigella, HT002, Actinomyces, Lachnospiraceae_unclassified, Limosilactobacillus, Eubacterium_coprostanoligenes_group_unclassified, Enterococcus, Blautia, Leuconostoc, Aerococcus, Secundilactobacillus, Lactiplantibacillus, Lachnospiraceae_UCG-009; there were 5 genera significantly enriched in the experimental group, including Clostridium, Dechloromonas, Gardnerella, Agitococcus_lubricus_group, Betaproteobacteria_unclassified. Among them, genera such as Staphylococcus, Escherichia-Shigella, Corynebacterium, Actinomyces, Enterococcus, Leuconostoc, Aerococcus, Secundilactobacillus, Lachnospiraceae_UCG-009, and Agitococcus_lubricus_group are all conditional pathogenic genera and are significantly enriched in the control group. The results show that the traditional Chinese medicine feed additive of the present invention can change the intestinal flora structure and reduce the abundance of harmful bacteria.

[0093] In summary, by adding the traditional Chinese medicine feed additive of the present invention to the basal diet, the intestinal flora structure of weaned piglets can be significantly improved, the abundance of harmful bacteria can be reduced, and the body immunity can be enhanced, thereby improving the diarrhea condition of piglets.

[0094] Example 2: Effects of Traditional Chinese Medicine Feed Additive and Comparative Products on Diarrhea and Mortality of Piglets

[0095] In the traditional Chinese medicine composition of the present invention, the compatibility of various herbs is reasonable, and the functions of each herb are fully exerted. All herbs work together in coordination to enhance the efficacy. Since there are a large number of experimental data in the present invention, the prescription has been adjusted and tested many times. Here, only the therapeutic effects of multiple groups of prescriptions on piglet diarrhea are listed and compared with the formula of the present invention.

[0096] Prescription of Comparative Example 1: 200 parts of Pulsatilla chinensis, 200 parts of Phellodendron amurense, 120 parts of Paeonia lactiflora, 120 parts of Aucklandia lappa, 120 parts of Citrus reticulata Blanco.

[0097] Prescription of Comparative Example 2: 200 parts of Pulsatilla chinensis, 200 parts of Phellodendron amurense, 150 parts of Atractylodes macrocephala, 90 parts of Glycyrrhiza uralensis.

[0098] Prescription of Example 1 of the present invention: 200 parts of Pulsatilla chinensis, 200 parts of Phellodendron amurense, 150 parts of Atractylodes macrocephala, 120 parts of Paeonia lactiflora, 120 parts of Aucklandia lappa, 120 parts of Citrus reticulata Blanco, 90 parts of Glycyrrhiza uralensis.

[0099] Prescription of a commercially available traditional Chinese medicine product as Comparative Example 3: Pulsatilla powder, Pulsatilla chinensis, Coptis chinensis, Phellodendron amurense, Cortex Fraxini (specific mass parts are unknown).

[0100] 1. Test objects and grouping

[0101] 120 piglets with yellow and white scour, similar in weight and age, were randomly divided into 4 groups, namely Group I, Group II, Group III, and Group IV, with 3 replicates in each group and 10 piglets in each replicate. Additive-containing feed was fed to each group for 5 consecutive days, and it was fed three times a day and allowed to eat freely, controlling the total feed intake of each piglet within about 300 g per day. Free drinking water was ensured during the test.

[0102] Group I: According to the prescription of Comparative Example 1, an additive accounting for 1% of the total feed mass was added to the basal diet;

[0103] Group II: According to the prescription of Comparative Example 2, an additive accounting for 1% of the total feed mass was added to the basal diet;

[0104] Group III: According to the prescription of Example 1 of the present invention, an additive accounting for 1% of the total feed mass was added to the basal diet;

[0105] Group IV: A commercially available traditional Chinese medicine product of Comparative Example 3 was taken, and an additive accounting for 1% of the total feed mass was added to the basal diet.

[0106] 2. Efficacy judgment criteria:

[0107] Cured: The phenomenon of yellow and white scour in piglets disappeared, the feces were formed, the food intake was normal, and the mental state was good;

[0108] Effective: The frequency of yellow and white scour in piglets decreased, the fecal characteristics were somewhat restored, and the food intake increased;

[0109] Invalid: The food intake and diarrhea symptoms did not improve, and the number of cases of yellow and white scour in piglets remained unchanged or even died compared with that before treatment.

[0110] 3. Test Results

[0111] The treatment effects of piglets in each group during the test are shown in Table 4.

[0112] Table 4 Treatment effects of each group on diarrhea of piglets with yellow and white scour

[0113]

[0114] Note: Different lowercase letters in superscript for the same row of data indicate significant differences when comparing Group III with Group I, Group II, and Group IV (P<0.05), and no superscript or the same lowercase letter in superscript indicates no significant difference (P>0.05).

[0115] It can be seen from the table that the effective number and cured number of Group III are significantly higher than those of Group I, Group II, and Group IV (P<0.05); the invalid number and death number of Group III are significantly lower than those of Group I, Group II, and Group IV (P<0.05). The results show that the traditional Chinese medicine feed additive of the present invention is superior to the commercially available traditional Chinese medicine products; moreover, when some of the traditional Chinese medicines in the traditional Chinese medicine composition of the present invention are missing, the ideal treatment effect cannot be achieved. Therefore, it can be proved that the traditional Chinese medicine composition of the present invention is obtained by reasonable compatibility according to the principle of syndrome differentiation and treatment of traditional Chinese medicine, giving full play to the synergistic effect between the medicinal materials, and none of the components in the formula can be missing.

[0116] The specific embodiments of the present invention have been described above. It should be understood that the present invention is not limited to the above specific embodiments, and those skilled in the art can make various deformations or modifications within the scope of the claims, which do not affect the essence of the present invention. Any equivalent implementation or change made without departing from the present invention shall be included within the scope of the technical solution of the present invention.

Claims

1. A traditional Chinese medicine feed additive for preventing and treating diarrhea in piglets, characterized in that, Taking Pulsatilla chinensis as the monarch drug, Phellodendron amurense as the minister drug, Atractylodes macrocephala, Paeonia lactiflora, Aucklandia lappa and Citrus reticulata Blanco as the assistant drugs together, and Glycyrrhiza uralensis as the courier drug; specifically, it is composed of the following raw materials in the following parts by weight: 190-210 parts of Pulsatilla chinensis, 190-210 parts of Phellodendron amurense, 140-160 parts of Atractylodes macrocephala, 110-130 parts of Paeonia lactiflora, 110-130 parts of Aucklandia lappa, 110-130 parts of Citrus reticulata Blanco, and 80-100 parts of Glycyrrhiza uralensis.

2. The traditional Chinese medicine feed additive according to claim 1, characterized in that, Specifically, it is composed of the following raw materials in the following parts by weight: 200 parts of Pulsatilla chinensis, 200 parts of Phellodendron amurense, 150 parts of Atractylodes macrocephala, 120 parts of Paeonia lactiflora, 120 parts of Aucklandia lappa, 120 parts of Citrus reticulata Blanco, and 90 parts of Glycyrrhiza uralensis.

3. The traditional Chinese medicine feed additive according to claim 1, characterized in that, The dosage form is powder, and the particle size range is 80-120 mesh.

4. The preparation method of the traditional Chinese medicine feed additive according to any one of claims 1 to 3, characterized in that, After weighing each raw material according to the said parts by weight, carry out drying treatment; after pulverization, sieve through an 80-120 mesh sieve, and mix evenly to obtain the said feed additive product.

5. Use of the traditional Chinese medicine feed additive described in any one of claims 1 to 3 in the preparation of a feed for preventing and treating diarrhea in piglets.

6. Use of the traditional Chinese medicine feed additive described in any one of claims 1 to 3 in the preparation of a feed for improving the intestinal microbial flora structure of piglets.

7. A feed for preventing and treating diarrhea in piglets, characterized in that, The feed contains the traditional Chinese medicine feed additive described in any one of claims 1 to 3, and the mass percentage of the traditional Chinese medicine feed additive in the feed is 0.5%-1%.

8. A feed for improving the intestinal microbial flora structure of piglets, characterized in that, The feed contains the traditional Chinese medicine feed additive described in any one of claims 1 to 3, and the mass percentage of the traditional Chinese medicine feed additive in the feed is 0.5%-1%.

9. The feed according to claim 7 or 8, characterized in that, In addition to the traditional Chinese medicine feed additive, it also includes a basic diet suitable for piglets; the components of the basic diet include corn, soybean meal and fish meal.

10. A method for using the feed according to claim 7 or 8, characterized in that, Including: Take an appropriate amount of feed and drinking water, stir to mix the traditional Chinese medicine feed additive and the basic diet evenly to obtain a feed for piglets to eat.

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