Houttuynia cordata composition for relieving respiratory tract discomfort and preparation method thereof

Abstract

The invention provides a heartleaf houttuynia herb composition for relieving respiratory tract discomfort, which is characterized by being prepared from the following raw materials in percentage by weight: 15-20% of heartleaf houttuynia herb extract, 6-12% of platycodon root extract, 0.5-1% of citrus peel volatile oil and the balance of pure water. The preparation method of the herba houttuyniae composition comprises the following steps: S1, uniformly mixing the herba houttuyniae extract, the radix platycodonis extract and the pure water in proportion, and filtering through a microfiltration membrane to obtain clear liquid; s2, adding the citrus peel volatile oil into the clarified liquid in proportion, and homogenizing to obtain uniform liquid; a preparation method of the houttuynia cordata extract comprises the following steps: a, selecting dry houttuynia cordata roots, crushing, and adding water; b, adding a fermentation strain for fermentation; and c, filtering the fermentation liquor, centrifuging, and passing through a membrane to obtain clear liquor. The herba houttuyniae composition for relieving respiratory tract discomfort can quickly and effectively relieve respiratory tract discomfort, and is remarkable in treatment effect and free of adverse side effects.

Description

Technical Field

[0001] The invention relates to the technical field of plant extract preparations, and in particular to a houttuynia cordata composition for relieving respiratory discomfort and a preparation method thereof. Background Art

[0002] As the process of modernization is gradually accelerating, a large number of tiny floating objects in people's living environment are inhaled into the human body, causing various effects on the respiratory tract and lungs. The human respiratory system is often invaded by various exogenous substances, causing respiratory discomfort, coughing, dry itching, swelling and other symptoms, which seriously affect people's normal life. Respiratory discomfort generally refers to infections, swelling, inflammation and other phenomena in the mouth, nose, throat, pharynx and other parts caused by external foreign objects or abnormalities in the human immune system. Generally, antibiotics, anti-inflammatory and other treatments are used according to different triggering factors.

[0003] Patent CN115666569A discloses a composition and method for preventing and / or treating viral infection or its related pathology, wherein the disclosed therapy involves administering an aminoquinoline drug, a corticosteroid and an antibiotic to an individual in need, specifically, providing such therapy before a substantial bacterial infection of the lung occurs. The method comprises: (a) administering an aminoquinoline drug to the individual; (b) administering an antibiotic to the individual; and (c) administering a corticosteroid (e.g., an inhalable corticosteroid) to the individual.

[0004] Patent CN104606166A discloses a compound cephalexin capsule and a preparation method thereof. The cephalexin capsule comprises the following raw material components: cephalexin (220-270 parts by weight), ambroxol (2-20 parts by weight), polacrilin potassium (25-125 parts by weight), chitosan oligosaccharide (50-100 parts by weight), talcum powder (5-50 parts by weight), and starch (10-75 parts by weight). Cephalexin is a β-lactam broad-spectrum antibiotic and a first-generation oral cephalosporin. It is suitable for most strains of gram-positive and gram-negative cocci, including Streptococcus pneumoniae, sensitive Staphylococcus aureus, Neisseria meningitidis, and gonococci. In clinical practice, cephalexin and ambroxol are used together to treat respiratory bacterial infections accompanied by viscous sputum, that is, to treat infections of sensitive bacteria. Mucus regulators can be used to make sputum easy to discharge, and at the same time, they have antibacterial and expectorant effects, and the therapeutic effect is significantly enhanced. Cephalexin and ambroxol are both drugs that have been used clinically for many years. They have definite efficacy, low incidence of adverse reactions, and are easy to take.

[0005] Usually, clinical treatment for respiratory discomfort is to take anti-inflammatory, antitussive, antibiotics and other drugs to relieve the symptoms. However, people have a certain resistance to taking drugs, infusions and other treatments. In addition, excessive use of antibiotics and anti-inflammatory drugs can easily cause drug resistance in the human body and increase the metabolic burden.

[0006] In view of this, the present invention is hereby provided. Summary of the Invention

[0007] The object of the present invention is to provide, in view of the deficiencies of the prior art, a houttuynia cordata composition for relieving respiratory discomfort and its preparation method. The houttuynia cordata composition has pure plant ingredients, is safe, mild, and easy to absorb, can quickly and effectively relieve respiratory discomfort, has a significant therapeutic effect, and has no adverse side effects.

[0008] In order to achieve the above object of the present invention, the following technical solutions are specifically adopted:

[0009] A houttuynia cordata composition for relieving respiratory discomfort is made from the following raw materials in weight percentages: 15% - 20% of houttuynia cordata extract, 6% - 12% of platycodon grandiflorum extract, 0.5% - 1% of citrus peel volatile oil, and the balance is pure water.

[0010] A preparation method of a houttuynia cordata composition for relieving respiratory discomfort includes the following steps: Step S1, uniformly mixing the houttuynia cordata extract, platycodon grandiflorum extract, and pure water in proportion, and passing through a microporous filter membrane to obtain a clarified liquid;

[0011] Step S2, adding the citrus peel volatile oil to the clarified liquid in proportion, and homogenizing to obtain a uniform liquid, which is the houttuynia cordata composition.

[0012] Further, the preparation method of the houttuynia cordata extract is as follows:

[0013] a. Select dried houttuynia cordata roots, crush them, and add water;

[0014] b. Add fermentation strains for fermentation;

[0015] c. The fermentation broth is filtered, centrifuged, and passed through a membrane to obtain a clarified liquid, which is the houttuynia cordata extract.

[0016] Further, the fermentation strains are one or a combination of several of acetic acid bacteria, bifidobacteria, bacillus subtilis, lactobacillus acidophilus, yeast, and lactic acid bacteria.

[0017] Further, the addition amount of the fermentation strains is 1.2% - 2% of the weight of the dried houttuynia cordata root powder.

[0018] Further, the fermentation strains are a combination of acetic acid bacteria, bacillus subtilis, and yeast, and the weight ratio of acetic acid bacteria: bacillus subtilis: yeast is 0.8 - 1:1 - 1.5:0.5 - 0.8.

[0019] Further, the fermentation condition is fermentation for 6 - 9 days at a temperature of 30 - 38°C.

[0020] Further, the preparation method of the platycodon grandiflorum extract is as follows:

[0021] a. Select fresh platycodon roots and add water;

[0022] b. Boil for extraction;

[0023] c. Concentrate the extract under reduced pressure, centrifuge to remove the precipitate, and thus obtain the platycodon extract.

[0024] Furthermore, the extraction time is 0.5 - 2 h, and the number of extraction times is 2 - 5 times.

[0025] Furthermore, the preparation method of the citrus peel volatile oil is as follows:

[0026] a. Select fresh citrus peels and crush them;

[0027] b. Perform steam distillation and ultrasonic-assisted extraction with 95% ethanol;

[0028] c. Concentrate the extraction solution under reduced pressure to recover ethanol, let it stand at low temperature, and take the upper layer of oily substance;

[0029] d. Filter with a filter membrane to obtain the citrus peel volatile oil.

[0030] Compared with the prior art, the beneficial effects of the present invention are as follows:

[0031] 1. The raw materials of the houttuynia cordata composition of the present invention are all plant extracts. The pure plant components are safe, mild, and easy to absorb, with relatively little irritation to the respiratory tract. They can quickly and effectively relieve respiratory discomfort, have a significant therapeutic effect, no adverse side effects, and will not cause drug resistance in the human body; the houttuynia cordata composition of the present invention contains a large amount of polysaccharide components and can also regulate the metabolic level in the body, promote metabolism, and will not increase the metabolic burden.

[0032] 2. The houttuynia cordata composition of the present invention can quickly relieve respiratory discomfort, significantly shorten the course of the disease, and achieve a high cure rate in a short time; the houttuynia cordata extract of the present invention ferments the dried roots of houttuynia cordata with specific fermentation strains, and the contents of its polysaccharide and flavonoid components are both significantly increased, and the anti-inflammatory and antioxidant levels are both improved, which is more conducive to relieving the discomfort of the respiratory tract; the citrus peel volatile oil of the present invention has an anti-inflammatory effect, can relieve the inflammatory reaction of the respiratory mucosa, and can also give a citrus aroma, with high patient acceptance.

[0033] 3. All the raw materials of the houttuynia cordata composition of the present invention are extracted separately and then compounded, without the need to additionally add excipients. The preparation process is simple and easy to operate. The prepared houttuynia cordata composition is a clear liquid without visible impurities, and can be directly sprayed on the oral cavity and respiratory tract and other parts through a pressure spray bottle or atomizer, and can directly and widely reach the respiratory mucosa to quickly relieve respiratory discomfort. Description of the Drawings

[0034] To more clearly illustrate the specific embodiments of the present invention or the technical solutions in the prior art, the following will briefly introduce the drawings required for the description of the specific embodiments or the prior art. Obviously, the drawings in the following description are some embodiments of the present invention. For those of ordinary skill in the art, without creative efforts, other drawings can also be obtained based on these drawings.

[0035] Figure 1 Process flow chart for the preparation method of the houttuynia cordata composition for relieving respiratory discomfort of the present invention;

[0036] Figure 2 Bar chart of the contents of polysaccharides and flavonoids before and after fermentation of the houttuynia cordata extract in Example 1 of the present invention;

[0037] Figure 3 Line chart of the total daily evaluation score of the application effect of the houttuynia cordata composition in Example 1 of the present invention. Specific embodiments

[0038] The following will clearly and completely describe the technical solutions of the present invention in combination with the drawings and specific embodiments. However, those skilled in the art will understand that the following described embodiments are some embodiments of the present invention, rather than all embodiments, and are only used to illustrate the present invention and should not be regarded as limiting the scope of the present invention. Based on the embodiments of the present invention, all other embodiments obtained by those of ordinary skill in the art without creative efforts fall within the scope of protection of the present invention. For those not specified in the embodiments, the conventional conditions or the conditions recommended by the manufacturer are followed. Those reagents or instruments not specified by the manufacturer can be obtained as conventional products through commercial purchase.

[0039] A houttuynia cordata composition for relieving respiratory discomfort is made from the following raw materials by weight percentage: 15% - 20% of houttuynia cordata extract, 6% - 12% of platycodon grandiflorum extract, 0.5% - 1% of citrus peel volatile oil, and the balance is pure water.

[0040] Among them, the mass percentage of the houttuynia cordata extract includes but is not limited to 15%, 16%, 17%, 18%, 19%, 20%, the mass percentage of the platycodon grandiflorum extract includes but is not limited to 6%, 7%, 8%, 9%, 10%, 11%, 12%, and the mass percentage of the citrus peel volatile oil includes but is not limited to 0.5%, 0.6%, 0.7%, 0.8%, 0.9%, 1%.

[0041] The preparation method of the houttuynia cordata composition for relieving respiratory discomfort includes the following steps: Step S1, uniformly mixing the houttuynia cordata extract, platycodon grandiflorum extract, and pure water in proportion, and passing through a 0.45 μm microporous filter membrane to obtain a clarified solution;

[0042] Step S2: Add citrus peel volatile oil to the clarified liquid in proportion, and after homogenization, a uniform liquid is obtained, which is the houttuynia cordata composition.

[0043] Preferably, the preparation method of the houttuynia cordata extract is as follows:

[0044] a. Select dried houttuynia cordata roots, crush them, and add 10 - 20 times the weight of water, and the multiple of water includes but is not limited to 10 times, 11 times, 12 times, 13 times, 14 times, 15 times, 16 times, 17 times, 18 times, 19 times, 20 times;

[0045] b. Add fermentation strains for fermentation. The addition amount of the fermentation strains is 1.2% - 2% of the weight of the dried houttuynia cordata root powder, including but not limited to 1.2%, 1.3%, 1.4%, 1.5%, 1.6%, 1.7%, 1.8%, 1.9%, 2.0%. Ferment at a temperature of 30 - 38°C for 6 - 9 days. The fermentation temperature includes but is not limited to 30°C, 32°C, 34°C, 36°C, 38°C, and the fermentation time includes but is not limited to 6 days, 6.5 days, 7 days, 7.5 days, 8 days, 8.5 days, 9 days;

[0046] c. The fermentation broth is filtered, centrifuged, and passed through a membrane to obtain a clarified liquid, which is the houttuynia cordata extract.

[0047] Preferably, the fermentation strains are one or a combination of acetic acid bacteria, bifidobacteria, bacillus subtilis, lactobacillus acidophilus, yeast, and lactic acid bacteria.

[0048] Preferably, the fermentation strains are a combination of acetic acid bacteria, bacillus subtilis, and yeast, and the weight ratio of acetic acid bacteria: bacillus subtilis: yeast is 0.8 - 1:1 - 1.5:0.5 - 0.8.

[0049] Preferably, the preparation method of the platycodon grandiflorum extract is as follows:

[0050] a. Select fresh platycodon grandiflorum roots and add 10 - 15 times the weight of water, and the multiple of water includes but is not limited to 10 times, 11 times, 12 times, 13 times, 14 times, 15 times;

[0051] b. Boil and extract for 0.5 - 2 h, including but not limited to 0.5 h, 1 h, 1.5 h, 2 h, and the extraction times are 2 - 5 times, including but not limited to 2 times, 3 times, 4 times, 5 times;

[0052] c. Concentrate the extract under reduced pressure to 1 / 3 - 2 / 3 of the original volume, including but not limited to 1 / 3, 1 / 2, 2 / 3, and centrifuge to remove the precipitate to obtain the platycodon grandiflorum extract.

[0053] Preferably, the preparation method of the citrus peel volatile oil is as follows:

[0054] a. Select fresh citrus peel and crush it;

[0055] b. By steam distillation and ultrasonic-assisted extraction with 95% ethanol;

[0056] c. The ethanol in the extract is recovered by reduced-pressure concentration, and the upper-layer oil is obtained by standing at low temperature;

[0057] d. The volatile oil of citrus peel is obtained by filtering with a 0.45 μm filter membrane.

[0058] Preferably, the citrus peel is selected from navel orange peel or tangerine peel, and tangerine peel is preferred.

[0059] Example 1

[0060] A houttuynia cordata composition for relieving respiratory discomfort is made from the following raw materials by weight percentage: 15% of houttuynia cordata extract, 6% of platycodon grandiflorum extract, 0.5% of volatile oil of citrus peel, and the balance is pure water.

[0061] The preparation method of the houttuynia cordata composition for relieving respiratory discomfort includes the following steps: Step S1, mixing the houttuynia cordata extract, platycodon grandiflorum extract, and pure water evenly according to the ratio, and passing through a 0.45 μm microporous filter membrane to obtain a clarified liquid;

[0062] Step S2, adding the volatile oil of citrus peel to the clarified liquid according to the ratio, and homogenizing to obtain a homogeneous liquid, thus obtaining the houttuynia cordata composition.

[0063] Among them, the preparation method of the houttuynia cordata extract is as follows:

[0064] a. Select dried houttuynia cordata roots, crush them, and add 10 times the weight of water;

[0065] b. Add fermentation strains Acetobacter aceti: Bacillus subtilis: Saccharomyces cerevisiae in a weight ratio of 0.8:1:0.5 for fermentation. The addition amount of the fermentation strains is 1.2% of the weight of the dried houttuynia cordata root powder, and ferment at 30 °C for 6 days;

[0066] c. The fermentation broth is filtered, centrifuged, and passed through a membrane to obtain a clarified liquid, thus obtaining the houttuynia cordata extract.

[0067] Among them, the preparation method of the platycodon grandiflorum extract is as follows:

[0068] a. Select fresh platycodon grandiflorum roots and add 10 times the weight of water;

[0069] b. Boil and extract for 0.5 h, and the extraction times are 2 times;

[0070] c. Concentrate the extract under reduced pressure to 1 / 3 of the original volume, and centrifuge to remove the precipitate, thus obtaining the platycodon grandiflorum extract.

[0071] Among them, the preparation method of the volatile oil of citrus peel is as follows:

[0072] a. Select fresh tangerine peel and crush it;

[0073] b. By steam distillation and ultrasonic-assisted extraction with 95% ethanol;

[0074] c. The extraction solution is concentrated under reduced pressure to recover ethanol, and the upper layer of oily substance is obtained by standing at low temperature;

[0075] d. The citrus peel volatile oil is obtained by filtering with a 0.45 μm filter membrane.

[0076] Example 2

[0077] A houttuynia cordata composition for relieving respiratory discomfort is made from the following raw materials by weight percentage: 16% of houttuynia cordata extract, 8% of platycodon grandiflorum extract, 0.6% of citrus peel volatile oil, and the balance is pure water.

[0078] The preparation method of the houttuynia cordata composition for relieving respiratory discomfort includes the following steps: Step S1, the houttuynia cordata extract, platycodon grandiflorum extract, and pure water are mixed evenly in proportion, and filtered through a 0.45 μm microporous filter membrane to obtain a clarified solution;

[0079] Step S2, adding citrus peel volatile oil to the clarified solution in proportion, and homogenizing to obtain a homogeneous liquid, thus obtaining the houttuynia cordata composition.

[0080] Among them, the preparation method of the houttuynia cordata extract is as follows:

[0081] a. Select dry houttuynia cordata roots, crush them, and add 12 times the weight of water;

[0082] b. Add fermentation strains Acetobacter aceti: Bacillus subtilis: Saccharomyces cerevisiae in a weight ratio of 0.8:1.2:0.6 for fermentation. The addition amount of the fermentation strains is 1.6% of the weight of the dry houttuynia cordata root powder, and ferment at 34 °C for 7 days;

[0083] c. The fermentation broth is filtered, centrifuged, and filtered through a membrane to obtain a clarified solution, thus obtaining the houttuynia cordata extract.

[0084] Among them, the preparation method of the platycodon grandiflorum extract is as follows:

[0085] a. Select fresh platycodon grandiflorum roots and add 12 times the weight of water;

[0086] b. Boil and extract for 1 h, and the extraction times are 3 times;

[0087] c. Concentrate the extraction solution under reduced pressure to 1 / 3 of the original volume, and centrifuge to remove the precipitate, thus obtaining the platycodon grandiflorum extract.

[0088] Among them, the preparation method of the citrus peel volatile oil is as follows:

[0089] a. Select fresh tangerine peel and crush it;

[0090] b. By steam distillation and ultrasonic-assisted extraction with 95% ethanol;

[0091] c. The extraction solution is concentrated under reduced pressure to recover ethanol, and the upper layer of oily substance is taken after standing at low temperature;

[0092] d. Filter with a 0.45μm filter membrane to obtain citrus peel volatile oil.

[0093] Example 3

[0094] A houttuynia cordata composition for relieving respiratory discomfort is made from the following raw materials by weight percentage: 18% of houttuynia cordata extract, 10% of platycodon grandiflorum extract, 0.8% of citrus peel volatile oil, and the balance is pure water.

[0095] The preparation method of the houttuynia cordata composition for relieving respiratory discomfort includes the following steps: Step S1, mix the houttuynia cordata extract, platycodon grandiflorum extract, and pure water in proportion and mix evenly, and pass through a 0.45μm microporous filter membrane to obtain a clarified liquid;

[0096] Step S2, add citrus peel volatile oil to the clarified liquid in proportion, and homogenize to obtain a homogeneous liquid, which is the houttuynia cordata composition.

[0097] Among them, the preparation method of the houttuynia cordata extract is as follows:

[0098] a. Select dried houttuynia cordata roots, crush them, and add 15 times the weight of water;

[0099] b. Add fermentation strains Acetobacter aceti: Bacillus subtilis: Saccharomyces cerevisiae in a weight ratio of 0.9:1.3:0.7 for fermentation. The addition amount of the fermentation strains is 1.8% of the weight of the dried houttuynia cordata root powder, and ferment at 36°C for 8 days;

[0100] c. The fermentation broth is filtered, centrifuged, and passed through a membrane to obtain a clarified liquid, which is the houttuynia cordata extract.

[0101] Among them, the preparation method of the platycodon grandiflorum extract is as follows:

[0102] a. Select fresh platycodon grandiflorum roots and add 14 times the weight of water;

[0103] b. Boil and extract for 1.5 h, and the extraction times are 4 times;

[0104] c. Concentrate the extraction solution under reduced pressure to 1 / 2 of the original volume, and centrifuge to remove the precipitate to obtain the platycodon grandiflorum extract.

[0105] Among them, the preparation method of the citrus peel volatile oil is as follows:

[0106] a. Select fresh navel orange peels and crush them;

[0107] b. By steam distillation and ultrasonic-assisted extraction with 95% ethanol;

[0108] c. The extraction solution is concentrated under reduced pressure to recover ethanol, and the upper layer of oily substance is taken after standing at low temperature;

[0109] d. Filter with a 0.45 μm filter membrane to obtain the volatile oil of citrus peel.

[0110] Example 4

[0111] A houttuynia cordata composition for relieving respiratory discomfort is made from the following raw materials by weight percentage: 20% of houttuynia cordata extract, 12% of platycodon grandiflorum extract, 1% of citrus peel volatile oil, and the balance is pure water.

[0112] The preparation method of the houttuynia cordata composition for relieving respiratory discomfort includes the following steps: Step S1, mix the houttuynia cordata extract, platycodon grandiflorum extract, and pure water evenly according to the ratio, and filter through a 0.45 μm microporous filter membrane to obtain a clarified liquid;

[0113] Step S2, add the citrus peel volatile oil to the clarified liquid according to the ratio, and homogenize to obtain a homogeneous liquid, then the houttuynia cordata composition is obtained.

[0114] Among them, the preparation method of the houttuynia cordata extract is as follows:

[0115] a. Select dry houttuynia cordata roots, crush them, and add 20 times the weight of water;

[0116] b. Add fermentation strains Acetobacter aceti: Bacillus subtilis: Saccharomyces cerevisiae in a weight ratio of 1:1.5:0.8 for fermentation. The addition amount of the fermentation strains is 2% of the weight of the dry houttuynia cordata root powder, and ferment at 38 °C for 9 days;

[0117] c. The fermentation broth is filtered, centrifuged, and passed through a membrane to obtain a clarified liquid, which is the houttuynia cordata extract.

[0118] Among them, the preparation method of the platycodon grandiflorum extract is as follows:

[0119] a. Select fresh platycodon grandiflorum roots and add 15 times the weight of water;

[0120] b. Boil and extract for 2 h, and the extraction times are 5 times;

[0121] c. Concentrate the extract under reduced pressure to 2 / 3 of the original volume, and centrifuge to remove the precipitate to obtain the platycodon grandiflorum extract.

[0122] Among them, the preparation method of the citrus peel volatile oil is as follows:

[0123] a. Select fresh navel orange peels and crush them;

[0124] b. Perform steam distillation and ultrasonic-assisted extraction with 95% ethanol;

[0125] c. The extraction solution is concentrated under reduced pressure to recover ethanol, and the upper layer of oily substance is taken after standing at low temperature;

[0126] d. Filter with a 0.45 μm filter membrane to obtain the volatile oil of citrus peel.

[0127] Comparative Example 1

[0128] The difference between the preparation method of this comparative example and Example 1 is that the fermentation strain is a single yeast.

[0129] Comparative Example 2

[0130] The difference between the preparation method of this comparative example and Example 1 is that platycodon root extract is not added.

[0131] Comparative Example 3

[0132] The difference between the preparation method of this comparative example and Example 1 is that the volatile oil of citrus peel is not added.

[0133] Test data and effect evaluation:

[0134] I. The phenol-sulfuric acid method was used to determine the polysaccharide content in the houttuynia cordata extract prepared in Example 1, and the rutin calibration method was used to determine the flavonoid content in the houttuynia cordata fermentation broth, and compared with the raw materials before fermentation. The data are shown in Table 1:

[0135] Table 1 Contents of polysaccharide and flavonoid in houttuynia cordata extract before and after fermentation in Example 1

[0136] Detection index Before fermentation After fermentation Polysaccharide (mg / mL) 26.63 40.19 Flavonoid (mg / mL) 1.78 2.03

[0137] Table 2 Contents of polysaccharide and flavonoid in houttuynia cordata extract before and after fermentation in Comparative Example 1

[0138] Detection index Before fermentation After fermentation Polysaccharide (mg / mL) 26.63 29.32 Flavonoid (mg / mL) 1.78 1.67

[0139] It can be seen from Table 1 and Table 2 that after the dry roots of houttuynia cordata are fermented, the contents of the active ingredients polysaccharide and flavonoid are significantly increased. In Example 1, the dry roots of houttuynia cordata were fermented with a specific fermentation strain, and in terms of the improvement amplitude of the polysaccharide and flavonoid contents, it was significantly better than that of fermenting the dry roots of houttuynia cordata with a single yeast in Comparative Example 1.

[0140] Polysaccharide has the effect of immune regulation, can enhance the body's own immune ability, and can also regulate the body's metabolic level and promote metabolism. Flavonoid has antioxidant effects, can effectively scavenge free radicals in the body, and also has anti-inflammatory properties, which can reduce the inflammatory response.

[0141] II. Take the houttuynia cordata composition for relieving respiratory discomfort prepared in Example 1 of the present invention, screen 10 qualified subjects with respiratory discomfort, directly spray on the oral cavity and respiratory tract parts, and evaluate the application effect by scoring. The effect of the composition was evaluated by cumulative scoring according to the time points through continuous use for 5 days.

[0142] Scoring criteria (3 - point system): 0 point - no improvement; 1 point - slight relief; 2 points - obvious relief; 3 points - basically cured. Total score for the day = 1 point × number of people scoring 1 point on that day + 2 points × number of people scoring 2 points on that day + 3 points × number of people scoring 3 points on that day

[0143] Table 3 Statistical table of scores for the evaluation of the application effect of the Houttuynia cordata composition in Example 1 by the subjects

[0144]

[0145]

[0146] As can be seen from Table 3, after the Houttuynia cordata composition of the present invention acts on the human respiratory tract, with the passage of time, the proportion of subjective effectiveness is getting higher and higher, that is, this product can relieve respiratory discomfort. On the first day, 4 subjects felt varying degrees of relief, indicating that the Houttuynia cordata composition of the present invention can quickly reach the respiratory mucosa and quickly exert its medicinal effect. By the third day, there were already subjects who were basically cured. By the fifth day, 60% of the subjects were basically cured and 30% of the subjects had obvious relief. It can be seen that the Houttuynia cordata composition of the present invention can quickly relieve and cure respiratory discomfort, significantly shorten the course of the disease, achieve a high cure rate in a short time, and the treatment effect is remarkable.

[0147] III. Evaluate the effect of the Houttuynia cordata composition of the present invention in relieving respiratory discomfort

[0148] The respiratory tract generally includes the trachea and the lungs, which belong to an open system and are easily stimulated by the outside world, triggering a series of oxidative stress and inflammatory reactions in the human body, and accompanied by reactions such as pain and cough. The antioxidant capacity (free radical scavenging ability) of the Houttuynia cordata composition of the present invention and its influence on the level of cellular inflammatory factors were evaluated through an in vitro model to assess the effect of the Houttuynia cordata composition of the present invention in relieving respiratory discomfort.

[0149] The specific experimental method is as follows:

[0150] 1. Evaluate the influence of the Houttuynia cordata composition of the present invention on the level of tissue cell inflammatory factors

[0151] (1) Experimental method

[0152] In this experiment, human bronchial epithelial cells BEAS - 2B were selected and cultured in a 96 - well plate until 80% confluence. The composition group, blank control group (culture medium), negative control group (untreated group), and positive control group (LPS - induced inflammatory model group) were set respectively. After treatment, the cell culture supernatant was collected, centrifuged to obtain the supernatant, and the detection of IL - 6 and TNF - α was carried out respectively according to the detection method of the ELISA kit.

[0153] (2) Experimental results

[0154] This experiment detected and analyzed the effects of Houttuynia cordata Thunb. composition on the levels of IL-6 and TNF-α in human bronchial epithelial cells BEAS-2B. The experimental results are shown in Table 4:

[0155] Table 4 Detection data of the effects on the levels of IL-6 and TNF-α in human bronchial epithelial cells BEAS-2B

[0156] Experimental group IL-6 (ng / mL) TNF-α (ng / mL) Blank control group 0.06±0.01 0.18±0.02 Negative control group 0.14±0.12 0.26±0.04 Positive control group 3.53±0.45 12.32±0.87 Houttuynia cordata composition group of Example 1 <![CDATA[2.23±0.22 * > <![CDATA[7.49±0.96 ** > Houttuynia cordata composition group of Example 2 <![CDATA[2.01±0.38 * > <![CDATA[7.43±0.45 ** > Houttuynia cordata composition group of Example 3 <![CDATA[1.97±0.41 * > <![CDATA[6.68±0.53 ** > Houttuynia cordata composition group of Example 4 <![CDATA[1.52±0.34 ** > <![CDATA[6.23±0.67 ** > Houttuynia cordata composition group of Comparative Example 2 3.13±0.36 11.01±0.84 Houttuynia cordata composition group of Comparative Example 3 3.26±0.29 <![CDATA[9.38±0.51 * >

[0157] Note: * indicates significant difference compared with the positive control group (p < 0.05), ** indicates extremely significant difference compared with the positive control group (p < 0.01).

[0158] As can be seen from Table 3, there was an extremely significant difference in the levels of IL-6 and TNF-α between the positive control group and the blank group (p < 0.01), indicating that the cell inflammation model induced by LPS was successfully established; compared with the positive control group, there was a significant difference in the decrease of IL-6 level in the Houttuynia cordata Thunb. composition group (p < 0.05), and an extremely significant difference in the decrease of TNF-α level (p < 0.01), indicating that the Houttuynia cordata Thunb. composition of the present invention has a certain inhibitory effect on the inflammatory response of human bronchial epithelial cells BEAS-2B, which is beneficial to relieve the discomfort of the respiratory tract; compared with Comparative Examples 2-3, the decrease in the levels of IL-6 and TNF-α in the Houttuynia cordata Thunb. composition groups of Examples 1-4 was significantly greater than that in Comparative Examples 2-3. It can be seen that the Houttuynia cordata Thunb. composition group of this application can effectively enhance the inhibitory effect on the inflammatory response of human bronchial epithelial cells BEAS-2B by compounding three components of Houttuynia cordata Thunb. extract, Platycodon grandiflorum extract and citrus peel volatile oil in a specific proportion.

[0159] 2. Evaluate the effect of the Houttuynia cordata Thunb. composition of the present invention on antioxidant capacity (free radical scavenging ability)

[0160] (1) Experimental method

[0161] DPPH free radical scavenging rate test

[0162] Preparation of DPPH solution: Weigh an appropriate amount of DPPH, dissolve it with absolute ethanol and make up the volume to 250 mL to prepare a solution with a concentration of 0.2 mmol / L.

[0163] Take 2 mL of the sample solution in a centrifuge tube, add 2 mL of DPPH solution, shake well and react in the dark for 15 min, then measure the absorbance A1 at 515 nm; take 2 mL of the sample and 2 mL of absolute ethanol and mix them evenly for reaction, measure the absorbance value A2 at 515 nm; take 2 mL of absolute ethanol and 2 mL of DPPH solution and mix them evenly for reaction, measure the absorbance value A0 at 515 nm.

[0164] The calculation of the DPPH free radical scavenging rate is shown in the formula:

[0165] DPPH free radical scavenging rate % = [1 - (A1 - A2) / A0] * 100%

[0166] (2) Experimental results

[0167] Table 5. DPPH free radical scavenging rates of the compositions provided by each example and comparative example

[0168]

[0169]

[0170] As can be seen from Table 5, with the change of the proportion of fermentation strains and the increase of the addition amount of strains in Examples 1 - 4, the DPPH free radical scavenging rate of the Houttuynia cordata composition is positively correlated; compared with Example 1, the single - strain fermentation in Comparative Example 1 has a worse effect on the DPPH free radical scavenging rate than the multi - strain fermentation; compared with Examples 2, 3 and 4, in Comparative Examples 2 and 3, for the compositions without Platycodon grandiflorum extract or citrus volatile oil, the DPPH free radical scavenging rate has decreased.

Claims

1. A houttuynia cordata composition for relieving respiratory discomfort, characterized in that It is made from the following raw materials by weight percentage: 15% - 20% of houttuynia cordata extract, 6% - 12% of platycodon grandiflorum extract, 0.5% - 1% of citrus peel volatile oil, and the balance is pure water.

2. A method for preparing the houttuynia cordata composition for relieving respiratory discomfort according to claim 1, characterized in that, It includes the following steps: Step S1, mix the houttuynia cordata extract, platycodon grandiflorum extract, and pure water evenly according to the ratio, and pass through a microporous filter membrane to obtain a clear liquid; Step S2, add the citrus peel volatile oil to the clear liquid according to the ratio, and homogenize to obtain a homogeneous liquid, then the houttuynia cordata composition is obtained.

3. The preparation method of the houttuynia cordata composition for relieving respiratory discomfort according to claim 2, characterized in that, The preparation method of the houttuynia cordata extract is as follows: a. Select dried houttuynia cordata roots, crush them, and add water; b. Add fermentation strains for fermentation; c. The fermentation broth is filtered, centrifuged, and passed through a membrane to obtain a clear liquid, which is the houttuynia cordata extract.

4. The preparation method of the houttuynia cordata composition for relieving respiratory discomfort according to claim 3, characterized in that, The fermentation strains are one or a combination of several of acetic acid bacteria, bifidobacteria, bacillus subtilis, lactobacillus acidophilus, yeasts, and lactic acid bacteria.

5. The preparation method of the houttuynia cordata composition for relieving respiratory discomfort according to claim 3, characterized in that, The addition amount of the fermentation strains is 1.2% - 2% of the weight of the dried houttuynia cordata root powder.

6. The preparation method of the houttuynia cordata composition for relieving respiratory discomfort according to claim 5, characterized in that, The fermentation strains are a combination of acetic acid bacteria, bacillus subtilis, and yeasts, and the weight ratio of acetic acid bacteria: bacillus subtilis: yeasts is 0.8 - 1:1 - 1.5:0.5 - 0.

8.

7. The preparation method of the houttuynia cordata composition for relieving respiratory discomfort according to claim 6, characterized in that, The fermentation condition is to ferment for 6 - 9 days at a temperature of 30 - 38°C.

8. The preparation method of the houttuynia cordata composition for relieving respiratory discomfort according to claim 2, wherein The preparation method of the platycodon grandiflorum extract is as follows: a. Select fresh platycodon grandiflorum roots and add water; b. Boil for extraction; c. Concentrate the extract under reduced pressure, centrifuge to remove the precipitate, and then the platycodon grandiflorum extract is obtained.

9. The preparation method of the houttuynia cordata composition for relieving respiratory discomfort according to claim 8, characterized in that, The extraction time is 0.5 - 2h, and the extraction times are 2 - 5 times.

10. The preparation method of the houttuynia cordata composition for relieving respiratory discomfort according to claim 2, characterized in that, The preparation method of the citrus peel volatile oil is as follows: a. Select fresh citrus peels and crush them; b. Perform steam distillation and ultrasonic-assisted extraction with 95% ethanol; c. Concentrate the extraction solution under reduced pressure to recover ethanol, and let it stand at low temperature to take the upper layer of oily substance; d. Filter with a filter membrane to obtain the citrus peel volatile oil.

Images