Compound preparation for improving laying rate of poultry and preparation method thereof

Abstract

The invention relates to a compound preparation for improving the laying rate of poultry. The feed additive comprises the following components in percentage by weight: 0.8-1.5% of tryptophan, 1.5-2.5% of glutamic acid, 3-6% of folium mori, 3-5% of red pepper, 8-12% of astragalus membranaceus, 5-8% of eucommia ulmoides, 3-5% of folium artemisiae argyi, 4-6% of hawthorn, 2-4% of leonurus, 3-5% of polygonum cuspidatum, 6-9% of herba epimedii, 5-8% of carrot powder, 1.5-3% of sodium humate, 8-10% of saccharomycetes, 0.005-0.008% of vitamin E, 0.05-0.1% of L-ascorbic acid, 2-4% of inulin and the balance of anhydrous dextrose, totaling 100%. The compound preparation also contains 32000-4000 IU / kg of vitamin D3 (vitamin D32000-4000 IU / kg). The compound preparation takes essential amino acids (tryptophan and glutamic acid) as a metabolism regulation core, and is matched with red pepper to promote nutrient absorption, herba epimedii to regulate endocrine, sodium humate to enhance immunity and vitamins D3 and E to improve reproductive function, and cooperates with nine Chinese herbal medicines such as astragalus membranaceus and eucommia ulmoides to form a'egg laying promotion-physique strengthening-stress resistance 'ternary system; the feed not only can supplement nutrition, improve reproductive system functions, enhance egg quality and increase laying rate, but also can improve immunity and anti-stress ability of laying hens.

Description

Technical Field

[0001] The present invention belongs to the technical field of feed additives, and mainly relates to a compound preparation for improving the egg production rate of poultry and a preparation method thereof. Background Art

[0002] Improving the egg production rate can significantly increase the economic benefits of poultry farming. In seasons with strong market demand, a high egg production rate can also enhance the advantage of farms in price competition. Improving the egg production rate can extend the peak egg production period and reduce the mortality and culling rate, thereby improving the overall production efficiency. In addition to quantity, the quality of eggs is also an important indicator for measuring production benefits. Improving the egg production rate can better meet social needs and promote the development of the laying hen farming industry.

[0003] There are various reasons for the decline in egg production rate: stress caused by environmental changes, nutritional problems caused by vitamin and trace element deficiencies or unbalanced feed nutrition, nutritional decline caused by feed mildew, and various reasons caused by multiple diseases, etc.

[0004] Tryptophan is an essential amino acid in animals. It not only participates in protein synthesis but also plays an important role in regulating the physiological functions of animals. For poultry, tryptophan can affect its nerve activities and indirectly affect the function of the reproductive system.

[0005] Glutamic acid can be used as an energy source by poultry and also participates in the regulation of various metabolic pathways in poultry, providing a necessary physiological basis for improving the egg production rate.

[0006] Mulberry leaves contain DNJ components and can regulate lipid metabolism and reduce egg yolk cholesterol.

[0007] Red peppers are rich in active ingredients such as capsaicin. Capsaicin has a certain degree of irritation and can stimulate the appetite of poultry, enabling poultry to consume more feed, thereby providing sufficient nutrients for egg production.

[0008] Astragalus membranaceus has the effect of enhancing the body's immunity, enabling poultry to resist the invasion of external pathogens and maintaining a healthy physical state, providing good physical conditions for egg production.

[0009] Eucommia ulmoides can regulate the endocrine system of poultry and affect the secretion of hormones, and hormones play a key role in the reproductive process of poultry.

[0010] Artemisia argyi has the effects of warming the meridians to stop bleeding and dispelling cold and relieving pain. In the compound preparation, it can improve the physique of poultry, especially for some poultry with a weak and cold physique.

[0011] Hawthorn helps to promote the digestive function of poultry and improve the utilization rate of feed.

[0012] Motherwort has a certain regulatory effect on the reproductive system of poultry and can promote the healthy development and normal function of the uterus.

[0013] Polygonum cuspidatum has multiple functions such as anti-inflammatory and antioxidant. It can reduce inflammatory responses in poultry and improve overall health.

[0014] Epimedium is a traditional Chinese herbal medicine for nourishing the kidney and strengthening yang, and plays a positive role in improving the reproductive performance of poultry.

[0015] Carrots are rich in nutrients such as carotene. Carotene can be converted into nutrients such as vitamin A in the body of poultry, and plays an important role in maintaining the vision, reproductive system, etc. of poultry.

[0016] Sodium humate has adsorption and chelation functions. It can absorb harmful substances in the intestines of poultry and purify the intestinal environment. It can also chelate some nutrients to improve their utilization rate in the body of poultry.

[0017] Yeast powder, rich in nutrients such as multiple vitamins and amino acids, is a high-quality nutritional supplement that can provide additional nutritional support for poultry and help improve egg production.

[0018] Vitamin D3 helps poultry absorb calcium, which is an important substance for the formation of eggshells. Vitamin E has an antioxidant effect and can protect poultry's reproductive cells from damage by free radicals.

[0019] Vitamin E, a fat-soluble antioxidant, protects cell membranes from free radical damage, maintains the integrity of the oviduct mucosa, and improves the secretion function of the eggshell gland; it works together with selenium to enhance the immunity of laying hens and reduce the incidence of salpingitis.

[0020] L-ascorbic acid, a water-soluble antioxidant, participates in collagen synthesis. It inhibits excessive secretion of cortisol during heat stress and reduces fluctuations in egg production; it promotes calcium ion absorption and transport and improves the problem of thin-shelled eggs.

[0021] Inulin, a water-soluble dietary fiber, selectively proliferates bifidobacteria. It can ferment to produce butyric acid, repair the intestinal villus structure; it can bind intestinal ammonia to reduce the concentration of harmful gases in the chicken house; it can regulate lipid metabolism and reduce the cholesterol content of egg yolks.

[0022] This formula takes essential amino acids (tryptophan and glutamate) as the core of metabolic regulation, and is combined with red pepper to promote nutrient absorption, epimedium to regulate endocrine, sodium humate to enhance immunity, vitamins D3 and E to enhance reproductive function, and 9 other Chinese herbal medicines such as astragalus and eucommia to form a "promoting egg production-strengthening physical fitness-anti-stress" ternary system.

[0023] The compatibility of the above drugs in this application can not only supplement nutrition, improve the function of the reproductive system, enhance egg quality, and increase the egg production rate, but also improve the immunity of laying hens and their stress resistance. The formula of this application is designed in a "trinity" manner of "optimizing natural components - enhancing efficiency through process orientation - coordinating regulation of multiple systems", forming significant technical advantages in terms of egg production performance, egg quality, and safety. At the same time, the raw materials used in the invention patent of this application are all green products that are safe, effective, and residue-free. Therefore, it is a very good product for farmers to increase the egg production rate. Summary of the Invention

[0024] The purpose of the present invention is to overcome the defects of the prior art and provide a compound preparation for improving the egg production rate of poultry. The compound preparation takes essential amino acids (tryptophan, glutamic acid) as the core of metabolic regulation, and is formulated with red pepper to promote nutrient absorption, epimedium to regulate endocrine, sodium humate to enhance immunity, vitamin D3 and E to enhance reproductive function, and synergistically combines 9 kinds of Chinese herbal medicines such as astragalus membranaceus and eucommia ulmoides to form a "promote egg production - strengthen physique - resist stress" ternary system; it can not only supplement nutrition, improve the function of the reproductive system, enhance egg quality, and increase the egg production rate, but also improve the immunity of laying hens and their stress resistance.

[0025] Another purpose of the present invention is to provide a preparation method of the above compound preparation.

[0026] The third purpose of the present invention is to provide the use of the above compound preparation in increasing the egg production rate.

[0027] To achieve the above purposes, the present invention adopts the following technical solutions: A compound preparation for increasing the egg production rate of poultry, the composition components and weight percentages of which are: tryptophan 0.8 - 1.5%, glutamic acid 1.5 - 2.5%, mulberry leaves 3 - 6%, red pepper 3 - 5%, astragalus membranaceus 8 - 12%, eucommia ulmoides 5 - 8%, mugwort leaf 3 - 5%, hawthorn 4 - 6%, motherwort 2 - 4%, polygonum cuspidatum 3 - 5%, epimedium 6 - 9%, carrot powder 5 - 8%, sodium humate 1.5 - 3%, yeast 8 - 10%, vitamin E 0.005 - 0.008%, L-ascorbic acid 0.05 - 0.1%, inulin 2 - 4%, anhydrous glucose added to 100%; The above percentages are calculated based on dry matter, and the total is 100%.

[0028] Further preferably, the yeast can be selenium-enriched yeast. Generally, selenium-enriched yeast with a selenium content of ≥1000 ppm is considered selenium-enriched yeast. The compound preparation also contains vitamin D3 2000 - 4000 IU / kg.

[0029] A preparation method of the above compound preparation for increasing the egg production rate of poultry, which includes the following steps: 1) Take mulberry leaves, red peppers, astragalus membranaceus, eucommia ulmoides, mugwort leaves, hawthorn fruits, motherwort herb, polygonum cuspidatum, epimedium herb, and carrots, and crush them separately to 80 - 100 mesh. Among them, red peppers, mugwort leaves, hawthorn fruits, and carrots are further moistened with 2 - 8% ethanol for 15 - 40 minutes; 2) Conduct three - stage gradient enzymatic hydrolysis; First stage: Take mulberry leaves, red peppers, astragalus membranaceus, eucommia ulmoides, mugwort leaves, hawthorn fruits, motherwort herb, polygonum cuspidatum, epimedium herb, and carrots and mix them to obtain mixture 1. Add cellulase and pectinase, mix well, then add purified water (the addition ratio of mixture 1 to purified water is preferably 1 g:10 - 15 mL), and adjust the pH to 5.0 - 5.5. Keep it at 45 ± 1 °C for 1.5 - 2.5 hours; Second stage: After the first stage ends, add xylanase and β - glucanase to the mixture, adjust the pH to 6.5 - 6.9, and keep it at 55 ± 1 °C for 1 - 2 hours; Third stage: After the second stage ends, add glucosidase and naringinase to the mixture, adjust the pH to 4 - 5, and keep it at 40 ± 1 °C for 0.5 - 1.5 hours; 3) Heat up to 80 - 95 °C, add L - ascorbic acid, maintain for 7 - 15 minutes, then cool down to 32 ± 1 °C. Mix with selenium - rich yeast and sodium humate, and then add bacillus subtilis and yeast. Keep it at 32 °C ± 1 °C for 0 - 12 h, and then keep it at 35 °C ± 1 °C for 12 - 36 h for fermentation; 4) Dry the fermentation product to a water content of less than 8%, crush and sieve it, then add tryptophan, glutamic acid, vitamin D3, vitamin E, inulin, and anhydrous glucose, and mix well to obtain the product.

[0030] Specifically, in step 2), it is preferred to use citric acid - disodium hydrogen phosphate buffer to adjust the pH to 5.0 - 5.5; use Tris - HCl buffer to adjust the pH to 6.5 - 6.9; use acetic acid - sodium acetate buffer to adjust the pH to 4 - 5.

[0031] Furthermore, in step 2), the addition amount of cellulase (enzyme activity ≥ 5000 U / g) accounts for 0.6 - 0.9% of the mass of mixture 1, the addition amount of pectinase (enzyme activity ≥ 3000 U / g) accounts for 0.3 - 0.6% of the mass of mixture 1. The addition amount of xylanase (enzyme activity ≥ 8000 U / g) accounts for 0.4 - 0.7% of the mass of mixture 1, the addition amount of β - glucanase (enzyme activity ≥ 4000 U / g) accounts for 0.2 - 0.4% of the mass of mixture 1. The addition amount of glucosidase (enzyme activity ≥ 2000 U / g) accounts for 0.3 - 0.5% of the mass of mixture 1, the addition amount of naringinase (enzyme activity ≥ 1500 U / g) accounts for 0.3 - 0.5% of the mass of mixture 1. The addition amount of bacillus subtilis accounts for 5 - 8% of the mass of mixture 1; the addition amount of yeast accounts for 8 - 10% of the mass of mixture 1.

[0032] The present invention also provides the application of the above compound preparation for improving the egg production rate of poultry in improving the egg production rate of poultry. Further, when the above compound preparation is applied or used, it is mixed in feed and the compound preparation is added at 1.0-1.2% of the quality of the complete feed, and it can be used continuously.

[0033] Compared with the prior art, the present invention has the following advantages and beneficial effects: (1) The present invention utilizes the dynamic pH regulation technology, adopts three buffer systems to precisely control the pH in stages, and through the progressive action of enzyme systems and temperature, the matching rate of the activity peak values of each enzyme system reaches more than 95%, which can significantly improve the extraction rate of active ingredients and reduce energy consumption; (2) The present invention regulates the egg production performance in multiple targets, avoids hormone dependence, and the enzymatic hydrolysis-fermentation coupling process fully improves the bioavailability of active ingredients; (3) The present invention can significantly improve the immunity of laying hens, improve the stress resistance, improve the intestinal health and reproductive system, regulate the hormones in the body, improve the egg production rate, and can also improve the egg production quality; (4) The compound preparation of the present invention is easily absorbed after entering the body, and is convenient for clinical administration, suitable for group administration, saving manpower and material resources; (5) The drug content of the present invention is clear, the administration is accurate, and the quality is controllable. Specific Embodiments

[0034] The technical solutions of the present invention will be further introduced in detail below in conjunction with embodiments, but the protection scope of the present invention is not limited thereto.

[0035] In the following embodiments, the selenium-enriched yeast used (selenium content: ≥2000 ppm) was purchased from Henan XUNUO Biotechnology Co., Ltd.

[0036] Cellulase (enzyme activity ≥5000 U / g) was purchased from Shandong Longda Bioengineering Co., Ltd.; Pectinase (enzyme activity ≥3000 U / g) was purchased from Jiangsu Ruiyang Biotechnology Co., Ltd.; Xylanase (enzyme activity ≥10000 U / g) was purchased from Guangdong Yiduoli Biotechnology Co., Ltd.; β-glucanase (enzyme activity ≥8000 U / g) was purchased from Guangdong Yiduoli Biotechnology Co., Ltd.; Glucosidase (enzyme activity ≥2000 U / g) was purchased from Shanghai Yuanye Biotechnology Co., Ltd.; Naringinase (enzyme activity ≥1500 U / g) was purchased from Shanghai Yuanye Biotechnology Co., Ltd.

[0037] Bacillus subtilis (viable count: ≥1×10 11CFU / g) was purchased from Angel Yeast Co., Ltd.; yeast (number of viable cells: ≥2×10^10 CFU / g) was purchased from Angel Yeast Co., Ltd.

[0038] Example 1 Preparation of a compound preparation for improving the egg production rate of chickens A compound preparation for improving the egg production rate of poultry, its composition and weight percentage are as follows: tryptophan 0.8%, glutamic acid 2.5%, mulberry leaves 3%, red pepper 5%, astragalus membranaceus 8%, eucommia ulmoides 8%, mugwort leaf 3%, hawthorn 6%, motherwort 2%, polygonum cuspidatum 5%, epimedium 6%, carrot powder 8%, sodium humate 1.5%, selenium-enriched yeast 10%, vitamin E (α-tocopherol) 0.008%, L-ascorbic acid 0.05%, inulin 4%, anhydrous glucose added to 100%; the compound preparation also contains vitamin D3 2000 IU / kg.

[0039] The preparation method of the above compound preparation for improving the egg production rate of poultry includes the following steps: 1. Take mulberry leaves, red pepper, astragalus membranaceus, eucommia ulmoides, mugwort leaf, hawthorn, motherwort, polygonum cuspidatum, epimedium, and carrot, and crush them to 80-100 meshes respectively. Among them, red pepper, mugwort leaf, hawthorn, and carrot are moistened with 5% ethanol (volume percentage, the same below) for 30 minutes.

[0040] 2. Three-stage gradient enzymatic hydrolysis The first stage: cellulose breaking Take the mixture of mulberry leaves, red pepper, astragalus membranaceus, eucommia ulmoides, mugwort leaf, hawthorn, motherwort, polygonum cuspidatum, epimedium, and carrot to obtain mixture 1, add cellulase and pectinase, stir evenly, then add purified water (the addition ratio of mixture 1 to purified water is 1g:12mL), and adjust the pH to 5.2 with citric acid-disodium hydrogen phosphate buffer, and keep it at 45±1°C for 2 hours.

[0041] Among them: the addition amount of cellulase accounts for 0.8% of the mass of mixture 1, and the addition amount of pectinase accounts for 0.5% of the mass of mixture 1.

[0042] The second stage: hemicellulose degradation After the first stage is completed, add xylanase and β-glucanase to the mixture, adjust the pH to 6.8 with Tris-HCl buffer, and keep it at 55±1°C for 1.5 hours.

[0043] Among them: the addition amount of xylanase accounts for 0.6% of the mass of mixture 1, and the addition amount of β-glucanase accounts for 0.3% of the mass of mixture 1.

[0044] The third stage: biological transformation After the second stage, glucosidase and naringinase are added to the mixture, and the pH is adjusted to 4.5 with acetic acid-sodium acetate buffer solution, and kept at 40 ± 1 °C for 1 hour.

[0045] Among them: the addition amount of glucosidase accounts for 0.4% of the mass of mixture 1, and the addition amount of naringinase accounts for 0.4% of the mass of mixture 1.

[0046] 3. Rapidly raise the temperature to 90 °C, immediately add L-ascorbic acid, maintain for 10 minutes and then lower the temperature to 32 ± 1 °C. After mixing with selenium-enriched yeast and sodium humate, add Bacillus subtilis and yeast, and keep at 32 °C ± 1 °C for 6 h and at 35 °C ± 1 °C for 24 h for fermentation. The addition amount of Bacillus subtilis accounts for 6% of the mass of mixture 1, and the addition amount of yeast accounts for 10% of the mass of mixture 1.

[0047] 4. Low-temperature dry the fermentation product at 40 - 50 °C until the water content is below 8%, add tryptophan, glutamic acid, vitamin D3, vitamin E, inulin, anhydrous glucose in proportion after pulverizing and sieving, and stir in a mixer for 20 minutes to obtain a compound preparation for improving the egg production rate of poultry.

[0048] Example 2 Preparation of a compound preparation for improving the egg production rate of poultry A compound preparation for improving the egg production rate of poultry, the composition components and weight percentages thereof are as follows: tryptophan 1.5%, glutamic acid 1.5%, mulberry leaf 6%, red pepper 3%, astragalus membranaceus 12%, eucommia ulmoides 5%, artemisia argyi 5%, hawthorn 4%, motherwort 4%, polygonum cuspidatum 3%, epimedium 9%, carrot powder 5%, sodium humate 3%, selenium-enriched yeast 8%, vitamin E (α-tocopherol) 0.005%, L-ascorbic acid 0.1%, inulin 2%, anhydrous glucose added to 100%; the compound preparation also contains 4000 IU / kg of vitamin D3.

[0049] The preparation method of the above compound preparation for improving the egg production rate of poultry includes the following steps: 1. Take mulberry leaf, red pepper, astragalus membranaceus, eucommia ulmoides, artemisia argyi, hawthorn, motherwort, polygonum cuspidatum, epimedium, carrot, and pulverize them to 80 - 100 meshes respectively. Among them, red pepper, artemisia argyi, hawthorn, and carrot are further wetted with 5% ethanol for 30 minutes.

[0050] 2. Three-stage gradient enzymatic hydrolysis The first stage: Cellulose breaking Take the mixture of mulberry leaf, red pepper, astragalus membranaceus, eucommia ulmoides, artemisia argyi, hawthorn, motherwort, polygonum cuspidatum, epimedium, carrot to obtain mixture 1, add cellulase and pectinase, stir evenly and then add purified water (the addition ratio of mixture 1 to purified water is 1 g:12 mL), and adjust the pH to 5.2 with citric acid-disodium hydrogen phosphate buffer solution, and keep at 45 ± 1 °C for 2 hours.

[0051] Among them: The addition amount of cellulase accounts for 0.8% of the mass of mixture 1, and the addition amount of pectinase accounts for 0.5% of the mass of mixture 1.

[0052] The second stage: Hemicellulose degradation After the first stage ends, xylanase and β-glucanase are added to the mixture, the pH is adjusted to 6.8 with Tris-HCl buffer solution, and it is maintained at 55 ± 1 °C for 1.5 hours.

[0053] Among them: The addition amount of xylanase accounts for 0.6% of the mass of mixture 1, and the addition amount of β-glucanase accounts for 0.3% of the mass of mixture 1.

[0054] The third stage: Biotransformation After the second stage ends, glucosidase and naringinase are added to the mixture, the pH is adjusted to 4.5 with acetic acid-sodium acetate buffer solution, and the temperature is maintained at 40 ± 1 °C for 1 hour.

[0055] Among them: The addition amount of glucosidase accounts for 0.4% of the mass of mixture 1, and the addition amount of naringinase accounts for 0.4% of the mass of mixture 1.

[0056] 3. Rapidly raise the temperature to 90 °C, immediately add L-ascorbic acid, maintain for 10 minutes and then lower the temperature to 32 ± 1 °C. After mixing with selenium-enriched yeast and sodium humate, add Bacillus subtilis and yeast, and maintain at 32 °C ± 1 °C for 6 h and at 35 °C ± 1 °C for 24 h for fermentation. The addition amount of Bacillus subtilis accounts for 5% of the mass of mixture 1, and the addition amount of yeast accounts for 8% of the mass of mixture 1.

[0057] 4. Low-temperature dry the fermentation product at 40 - 50 °C until the water content is below 8%. After pulverizing and sieving, add tryptophan, glutamic acid, vitamin D3, vitamin E, inulin, and anhydrous glucose, and place them in a mixer according to the ratio and stir for 20 minutes to obtain the compound preparation for improving the egg production rate of poultry.

[0058] Example 3 Preparation of the compound preparation for improving the egg production rate of poultry A compound preparation for improving the egg production rate of poultry, its composition components and weight percentages are as follows: tryptophan 1.1%, glutamic acid 2.0%, mulberry leaf 4.5%, red pepper 4%, astragalus membranaceus 10%, eucommia ulmoides 6.5%, mugwort leaf 4%, hawthorn 5%, motherwort 3%, polygonum cuspidatum 4%, epimedium 7.5%, carrot powder 6.5%, sodium humate 2%, selenium-enriched yeast 9%, vitamin E (α-tocopherol) 0.0065%, L-ascorbic acid 0.07%, inulin 3%, anhydrous glucose added to 100%; the compound preparation also contains vitamin D3 3000 IU / kg.

[0059] The preparation method of the above compound preparation for improving the egg production rate of poultry includes the following steps: 1. Take mulberry leaves, red peppers, astragalus, eucommia, mugwort, hawthorn, motherwort, polygonum cuspidatum, epimedium, and carrots, and crush them to 80-100 meshes respectively. Among them, red peppers, mugwort, hawthorn, and carrots are further wetted with 5% ethanol for 30 minutes.

[0060] 2. Three-stage gradient enzymatic hydrolysis The first stage: Cellulose breaking Take the mixed mulberry leaves, red peppers, astragalus, eucommia, mugwort, hawthorn, motherwort, polygonum cuspidatum, epimedium, and carrots to obtain mixture 1, add cellulase and pectinase, stir evenly, then add purified water (the addition ratio of mixture 1 to purified water is 1g: 12mL), and adjust the pH to 5.2 with citric acid-disodium hydrogen phosphate buffer, and maintain the temperature at 45±1°C for 2 hours.

[0061] Among them: The addition amount of cellulase accounts for 0.8% of the mass of mixture 1, and the addition amount of pectinase accounts for 0.5% of the mass of mixture 1.

[0062] The second stage: Hemicellulose degradation After the first stage, add xylanase and β-glucanase to the mixture, adjust the pH to 6.8 with Tris-HCl buffer, and maintain at 55±1°C for 1.5 hours.

[0063] Among them: The addition amount of xylanase accounts for 0.6% of the mass of mixture 1, and the addition amount of β-glucanase accounts for 0.3% of the mass of mixture 1.

[0064] The third stage: Biotransformation After the second stage, add glucosidase and naringinase to the mixture, adjust the pH to 4.5 with acetic acid-sodium acetate buffer, and maintain at 40±1°C for 1 hour.

[0065] Among them: The addition amount of glucosidase accounts for 0.4% of the mass of mixture 1, and the addition amount of naringinase accounts for 0.4% of the mass of mixture 1.

[0066] 3. Rapidly raise the temperature to 90°C, immediately add L-ascorbic acid, maintain for 10 minutes and then cool to 32±1°C. Mix with selenium-enriched yeast and sodium humate, then add Bacillus subtilis and yeast, and maintain at 32°C±1°C for 6h and at 35°C±1°C for 24h for fermentation. The addition amount of Bacillus subtilis accounts for 7% of the mass of mixture 1, and the addition amount of yeast accounts for 9% of the mass of mixture 1.

[0067] 4. Low-temperature dry the fermentation product at 40 - 50 °C until the water content is below 8%. After pulverizing and sieving, add tryptophan, glutamic acid, vitamin D3, vitamin E, inulin, and anhydrous glucose, and place them in a mixer according to the proportion and stir for 20 minutes to obtain the compound preparation for improving the egg production rate of poultry.

[0068] Example 4 Preparation of the reference compound preparation for improving the egg production rate of poultry A reference compound preparation for improving the egg production rate of poultry, its composition components and weight percentages are as follows: tryptophan 1.1%, glutamic acid 2.0%, mulberry leaf 4.5%, red pepper 4%, astragalus membranaceus 10%, eucommia ulmoides 6.5%, mugwort leaf 4%, hawthorn 5%, motherwort 3%, polygonum cuspidatum 4%, epimedium 7.5%, carrot powder 6.5%, sodium humate 2%, selenium-enriched yeast 9%, vitamin E (α-tocopherol) 0.0065%, L-ascorbic acid 0.07%, inulin 3%, anhydrous glucose added to 100%; the compound preparation also contains vitamin D3 3000 IU / kg.

[0069] The preparation method of the above-mentioned reference compound preparation for improving the egg production rate of poultry includes the following steps: 1. Take mulberry leaf, red pepper, astragalus membranaceus, eucommia ulmoides, mugwort leaf, hawthorn, motherwort, polygonum cuspidatum, epimedium, and carrot, and pulverize them to 80 - 100 mesh respectively.

[0070] 2. Add cellulase, pectinase, xylanase, β-glucanase, glucosidase, and naringinase to the above traditional Chinese medicines, mix evenly, keep at 45 ± 1 °C for 2 hours, mix with selenium-enriched yeast and sodium humate, then add bacillus subtilis and yeast, and keep at 32 °C ± 1 °C for 6 h and at 35 °C ± 1 °C for 24 h for fermentation. Among them, the addition amounts of cellulase, pectinase, xylanase, β-glucanase, glucosidase, naringinase, bacillus subtilis, and yeast refer to Example 3.

[0071] 4. Low-temperature dry the fermentation product at 40 - 50 °C until the water content is below 8%. After pulverizing and sieving, add tryptophan, glutamic acid, vitamin D3, vitamin E, L-ascorbic acid, inulin, and anhydrous glucose, and place them in a mixer according to the proportion and stir for 20 minutes to obtain it.

[0072] Note: Gradient enzymolysis method is not adopted in this control.

[0073] Example 5 Preparation of the reference compound preparation for improving the egg production rate of poultry A reference substance of a compound preparation for improving the egg production rate of poultry, and its composition and weight percentage are as follows: tryptophan 1.1%, glutamic acid 2.0%, mulberry leaves 4.5%, red pepper 4%, astragalus membranaceus 10%, eucommia ulmoides 6.5%, mugwort leaves 4%, hawthorn 5%, motherwort 3%, polygonum cuspidatum 4%, epimedium 7.5%, carrot powder 6.5%, sodium humate 2%, selenium-enriched yeast 9%, vitamin E (α-tocopherol) 0.0065%, L-ascorbic acid 0.07%, inulin 3%, anhydrous glucose added to 100%; the compound preparation also contains 3000 IU / kg of vitamin D3.

[0074] The preparation method of the above reference substance of the compound preparation for improving the egg production rate of poultry includes the following steps: 1. Take mulberry leaves, red pepper, astragalus membranaceus, eucommia ulmoides, mugwort leaves, hawthorn, motherwort, polygonum cuspidatum, epimedium, and carrots, and crush them to 80-100 meshes respectively.

[0075] 2. Add selenium-enriched yeast and sodium humate to the above traditional Chinese medicines, mix them evenly, then add bacillus subtilis and yeast, and keep fermenting at 35°C ± 1°C for 36 hours. Among them, the addition amounts of bacillus subtilis and yeast refer to Example 3.

[0076] 3. After the fermentation is completed, dry the fermentation product at a low temperature of 40-50°C until the water content is below 8%, crush and sieve it, and then add tryptophan, glutamic acid, vitamin D3, vitamin E, L-ascorbic acid, inulin, and anhydrous glucose, and stir in a mixer for 20 minutes according to the ratio to obtain the product.

[0077] Note: The enzymatic hydrolysis method is not used in this control.

[0078] Example 6. Comparative test of the compound preparation of the present invention on improving the egg production rate of poultry (I) Test drugs Test groups 1, 2, and 3: corresponding to the compound preparations prepared in Examples 1, 2, and 3 respectively; Control group 1: the reference substance of the compound preparation prepared in Example 4; Control group 2: the reference substance of the compound preparation prepared in Example 5.

[0079] (II) Test methods (1) Test design: Select 1800 300-day-old Hy-Line Brown laying hens and randomly divide them into 6 groups. The test time is 30 days.

[0080] The diet of the test group is the basic diet + 1% of the compound preparation of the example; Control group 1 is the basic diet + 1% of the reference substance of the compound preparation in Example 4; Control group 1 is the basic diet + 1% of the reference substance of the compound preparation in Example 5; Other management shall be carried out in accordance with the large-scale laying hen production management system.

[0081] (2) Measurement indicators: laying rate, average egg weight, egg production, feed-to-egg ratio, feed consumption, shell-breaking rate.

[0082] (3) Test results Table 1 Laying hen production performance As can be seen from Table 1, the average laying rate of the experimental group is 92.1%, which is 1.9%, 3.8% and 10.8% higher than that of Control Group 1, Control Group 2 and the blank control group respectively; the average feed-to-egg ratio of the experimental group is 2.15, which is 0.10, 0.16 and 0.28 lower than that of Control Group 1, Control Group 2 and the blank control group respectively; the average egg weight of the experimental group is 62.8 g, which is 2.6%, 4.1% and 7.0% higher than that of Control Group 1, Control Group 2 and the blank control group respectively.

[0083] From the above data, it can be seen that the formula adopted by the product of the present invention can significantly improve the poultry laying rate, and the gradient enzymolysis method adopted in the process can significantly improve the use effect of the product.

Claims

1. A compound preparation for improving the egg production rate of poultry, characterized in that, Its composition and weight percentage are as follows: tryptophan 0.8 - 1.5%, glutamic acid 1.5 - 2.5%, mulberry leaves 3 - 6%, red pepper 3 - 5%, astragalus membranaceus 8 - 12%, eucommia ulmoides 5 - 8%, mugwort leaves 3 - 5%, hawthorn 4 - 6%, motherwort 2 - 4%, polygonum cuspidatum 3 - 5%, epimedium 6 - 9%, carrot powder 5 - 8%, sodium humate 1.5 - 3%, yeast 8 - 10%, vitamin E 0.005 - 0.008%, L - ascorbic acid 0.05 - 0.1%, inulin 2 - 4%, anhydrous glucose added to 100%; The yeast described is selenium - rich yeast.

2. The compound preparation for improving the egg production rate of poultry according to claim 1, wherein The compound preparation also contains vitamin D3 2000 - 4000 IU / kg.

3. The preparation method of the compound preparation for improving the egg production rate of poultry according to claim 1 or 2, characterized in that, It includes the following steps: 1) Take mulberry leaves, red pepper, astragalus membranaceus, eucommia ulmoides, mugwort leaves, hawthorn, motherwort, polygonum cuspidatum, epimedium, and carrot, and crush them to 80 - 100 meshes respectively. Among them, red pepper, mugwort leaves, hawthorn, and carrot are moistened with 2 - 8% ethanol for 15 - 40 minutes; 2) Conduct three - stage gradient enzymatic hydrolysis; The first stage: Take the mixture of mulberry leaves, red pepper, astragalus membranaceus, eucommia ulmoides, mugwort leaves, hawthorn, motherwort, polygonum cuspidatum, epimedium, and carrot to obtain mixture 1, add cellulase and pectinase, mix well, then add purified water, and adjust the pH to 5.0 - 5.5, and keep it at 45 ± 1 °C for 1.5 - 2.5 hours; The second stage: After the first stage ends, add xylanase and β - glucanase to the mixture, adjust the pH to 6.5 - 6.9, and keep it at 55 ± 1 °C for 1 - 2 hours; The third stage: After the second stage ends, add glucosidase and naringinase to the mixture, adjust the pH to 4 - 5, and keep it at 40 ± 1 °C for 0.5 - 1.5 hours; 3) Heat up to 80 - 95 °C, add L - ascorbic acid, maintain for 7 - 15 minutes and then cool down to 32 ± 1 °C. Mix with selenium - rich yeast and sodium humate, then add bacillus subtilis and yeast, keep it at 32 °C ± 1 °C for 0 - 12 h, and then keep it at 35 °C ± 1 °C for 12 - 36 h for fermentation; 4) Dry the fermentation product to a water content of less than 8%, crush and sieve it, then add tryptophan, glutamic acid, vitamin D3, vitamin E, inulin, and anhydrous glucose, and mix well to obtain the product.

4. The preparation method of the compound preparation for improving the egg production rate of poultry according to claim 3, characterized in that, In step 2), use citric acid - disodium hydrogen phosphate buffer solution to adjust the pH to 5.0 - 5.5; use Tris - HCl buffer solution to adjust the pH to 6.5 - 6.9; use acetic acid - sodium acetate buffer solution to adjust the pH to 4 - 5.

5. The preparation method of the compound preparation for improving the egg production rate of poultry according to claim 3, characterized in that, In step 2), the addition amount of cellulase accounts for 0.6 - 0.9% of the mass of mixture 1, and the addition amount of pectinase accounts for 0.3 - 0.6% of the mass of mixture 1.

6. The preparation method of the compound preparation for improving the egg production rate of poultry according to claim 3, characterized in that, In step 2), the addition amount of xylanase accounts for 0.4 - 0.7% of the mass of mixture 1, and the addition amount of β - glucanase accounts for 0.2 - 0.4% of the mass of mixture 1.

7. The preparation method of the compound preparation for improving the egg production rate of poultry according to claim 3, characterized in that, In step 2), the addition amount of glucosidase accounts for 0.3 - 0.5% of the mass of mixture 1, and the addition amount of naringinase accounts for 0.3 - 0.5% of the mass of mixture 1.

8. The preparation method of the compound preparation for improving the egg production rate of poultry according to claim 3, characterized in that, In step 2), the addition amount of Bacillus subtilis accounts for 5-8% of the mass of mixture 1; the addition amount of yeast accounts for 8-10% of the mass of mixture 1.

9. Use of the compound preparation according to claim 1 or 2 for improving the egg production rate of poultry in improving the egg production rate of poultry.

10. The application according to claim 9, characterized in that, For mixed feeding, add the compound preparation at 1.0-1.2% of the mass of the complete feed.

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