Pseudo-ginseng medicinal material processing method based on ultralow-temperature crushing

Abstract

The invention discloses a pseudo-ginseng medicinal material processing method based on ultralow temperature crushing, which combines a gradient cooling technology and a step-by-step crushing technology, and is suitable for processing pseudo-ginseng and other traditional Chinese medicinal materials rich in heat-sensitive active ingredients (such as saponin, polysaccharide and the like). The staged freezing and crushing technology has remarkable advantages in pseudo-ginseng processing, including protection of active ingredients, improvement of crushing efficiency, improvement of powder quality, reduction of energy consumption, improvement of process controllability and the like. By controlling the temperature and crushing parameters in stages, efficient and low-loss pseudo-ginseng processing can be realized, and technical support is provided for modern production of traditional Chinese medicines.

Description

Technical Field

[0001] The present invention relates to the technical field of Chinese medicinal material processing, and particularly relates to a processing method for Panax notoginseng medicinal materials based on cryogenic grinding. Background Art

[0002] Compound Xueshuantong Capsule is one of the exclusive varieties of Guangdong Zhongsheng Pharmaceutical Co., Ltd., and is a Chinese patent medicine for the treatment of cardiovascular and cerebrovascular diseases. Guided by traditional Chinese medicine theory and refined with modern pharmaceutical technology, this product has the effects of promoting blood circulation to remove blood stasis and replenishing qi and nourishing yin, and is widely used clinically for the treatment of cardiovascular and cerebrovascular diseases such as cerebral infarction and angina pectoris of coronary heart disease.

[0003] Compound Xueshuantong Capsule selects authentic Chinese medicinal materials such as Panax notoginseng, Astragalus membranaceus, and Salvia miltiorrhiza to ensure high-quality medicinal materials and stable drug efficacy. In the formula, Panax notoginseng promotes blood circulation to remove blood stasis and reduces swelling and alleviates pain. Its main active ingredients include saponins, polysaccharides, flavonoids, amino acids, volatile oils, etc. Among them, the saponins are mainly ginsenoside Rb1, Rb2, Rc, Rd, Re, Rg1, Rg2, Rh1, R1, etc., which have a variety of pharmacological effects, including anti-inflammatory, antioxidant, anti-tumor, anti-fatigue, improving cardiovascular function, enhancing immunity, etc.

[0004] In the 2020 edition of the Chinese Pharmacopoeia, there are clear requirements for each component of Compound Xueshuantong Capsule. Among them, for Panax notoginseng, each capsule of Compound Xueshuantong Capsule contains Panax notoginseng, calculated as the total amount of ginsenoside Rg1 (C 42 H 72 O 14 ), ginsenoside Rb1 (C 54 H 92 O 23 ), ginsenoside Re (C 48 H 82 O 18 ) and notoginsenoside R1 (C 47 H 80 O 18 ), shall not be less than 9.5 mg. Regarding the preparation method, after Panax notoginseng is ground, it is impregnated and extracted with ethanol, concentrated into clear paste for standby, the medicinal residues are dried, ground into fine powder, and reserved for use.

[0005] The traditional processing method of Panax notoginseng powder is to wash fresh Panax notoginseng and then dry it in the sun, by drying or roasting to obtain dry Panax notoginseng, and then further grind it to obtain Panax notoginseng powder. During the grinding process, traditional mechanical grinders such as hammer mills and blade mills are used. This method has high efficiency, but may generate high temperature, affecting the thermosensitive components in Panax notoginseng.

[0006] Chinese patent CN201310594822.2 discloses a method for ultrafine grinding of Panax notoginseng, which includes first cleaning Panax notoginseng, then punching Panax notoginseng into coarse particles by mechanical punching, and then ultrafine grinding the punched coarse particles into ultrafine powder. Compared with the traditional method, the processing cycle of Panax notoginseng powder is greatly shortened, the production efficiency is improved, and the produced Panax notoginseng powder is more delicate and of good quality. However, during the ultrafine grinding process, mechanical energy is converted into heat energy, which may cause the material temperature to rise. For heat-sensitive materials (such as saponins, polysaccharides and other active ingredients in Panax notoginseng), high temperature may cause degradation or inactivation of the effective ingredients.

[0007] Chinese patent CN202411499166.2 discloses an automated equipment for low-temperature crushing of Panax notoginseng powder and its working method. Through the cold air pipe, an external refrigerator can further introduce cold air into the second silo to reduce the internal temperature of the second silo. Through the blocking of the air flow by the baffle, the cold air introduced into the first silo first contacts the bottom of the crushing disk, and is guided along the bottom of the crushing disk and the baffle to the screw teeth and hammer block. The introduced cold air can take away the heat generated on the hammer block, tooth plate and screw teeth to the greatest extent, thereby improving the effect of crushing Panax notoginseng. This patent only discloses the crushing process, and does not conduct relevant research on the freezing step before crushing.

[0008] From the above, we can see that the current low-temperature crushing technology can effectively retain the heat-sensitive components in Panax notoginseng and reduce the loss of effective ingredients. However, most of the current research on Panax notoginseng freezing and crushing only focuses on the crushing process, while there are few reports on the freezing step before the crushing process. In addition, the overall control of the crushing process is too simple and extensive, which affects the crushing effect. Summary of the invention

[0009] The purpose of the present invention is to overcome the shortcomings of the prior art and provide a Panax notoginseng medicinal material processing method based on ultra-low temperature crushing. The method consists of a preparation stage, a pre-freezing stage, a deep freezing stage, an ultra-low temperature stage and a crushing stage. Compared with the general freezing and crushing method, the temperature in each stage of the present invention is precisely controlled to avoid temperature fluctuations causing changes in material properties. The processed medicinal materials are not only uniform and stable, but also can significantly improve the content of the index components specified in the 2020 edition of the Panax notoginseng "Chinese Pharmacopoeia", providing qualified raw medicinal materials for later diversified processing.

[0010] The purpose of the present invention is achieved through the following technical solutions:

[0011] A method for processing Panax notoginseng medicinal material based on ultra-low temperature pulverization comprises the following steps:

[0012] 1) Preparation stage: Remove the rhizomes, young shoots, fibrous roots and mud from Panax notoginseng, wash it clean, drain it, chop it into granules while it is fresh, and sieve it.

[0013] 2) Pre-freezing stage: Place the pseudo-ginseng in a biological low-temperature refrigerator for pre-freezing at 0°C to -20°C for 10 - 20 minutes.

[0014] 3) Deep-freezing stage: From -20°C to -40°C, cool down at a rate of 1 - 2°C / min for 20 - 30 minutes.

[0015] 4) Ultra-low temperature stage: From -40°C to -80°C, cool down at a rate of 2 - 4°C / min for 10 - 20 minutes.

[0016] 5) Crushing stage: Maintain at -80°C to -100°C. The first crushing speed is 1500 - 2000 rpm for 5 - 10 minutes, and the second crushing speed is 2500 - 3000 rpm for 10 - 15 minutes. Collect the powder, sieve it through 80 - 200 meshes, seal and package it, and store the packaged powder in a low-temperature environment below -20°C.

[0017] In the variable-temperature freezing process of the present invention, it refers to starting from a specific temperature, cooling at a specific variable temperature rate to the termination temperature, and then maintaining the termination temperature to continue freezing / drying. For example, in the deep-freezing stage of the aforementioned process step 3) "-20°C to -40°C, cool down at a rate of 1 - 2°C / min for 20 - 30 minutes", it means that this stage starts from -20°C, cools down at a rate of 1 - 2°C / min to the termination temperature of -40°C, and then maintains the temperature of -40°C for a total duration of 20 - 30 minutes.

[0018] In the present invention, step-by-step freezing before crushing is one of the key technologies to achieve the technical effects. By adopting gradient cooling combined with low-temperature crushing technology, it aims to maximize the protection of the active ingredients (such as saponin components) in pseudo-ginseng, while improving the crushing efficiency and product quality. Through gradient cooling, the material temperature is gradually reduced to reduce the loss of heat-sensitive components. In the pre-freezing stage, from 0°C to -20°C, slow cooling can reduce the formation of ice crystals inside and outside the cells, avoid cell structure damage, prevent the loss of saponin components due to cell rupture, achieve the effect of initially protecting saponin components and reducing heat-sensitive degradation. In the deep-freezing stage, from -20°C to -40°C, the temperature is further reduced to form tiny ice crystals in the material, reduce the damage to the cell wall during mechanical crushing, achieve the effect of protecting the integrity of saponin components and preventing the loss of components caused by cell rupture. In the ultra-low temperature stage, from -40°C to -80°C, the material enters the vitrified state, reduces the mechanical damage of ice crystals to the cell structure, and at the same time inhibits the activity of enzymes to prevent the enzymatic hydrolysis of saponin components, achieving the maximum protection of the structure and activity of saponin components. In the crushing stage, from -80°C to -100°C, crushing is carried out in an ultra-low temperature environment to avoid thermal degradation of saponin components caused by friction heating, and ensure the stability and biological activity of saponin components.

[0019] In the present invention, the cooling rate is one of the key technologies for achieving the technical effects. The cooling rate has a significant impact on the cell structure of Panax notoginseng, the retention rate of active ingredients, and the pulverization effect. With rapid cooling, the ice crystals are smaller, resulting in less mechanical damage to cell walls and cell membranes, better protection of saponin components, and reduced loss due to cell rupture, but it has a higher dependence on equipment. With slow cooling, larger ice crystals are formed in the intracellular water, leading to damage to the cell structure and possibly causing the leakage of saponin components from the ruptured cells, reducing the retention rate. Gradient cooling can both reduce ice crystal damage and improve the pulverization efficiency. Specifically, in the processing method of Panax notoginseng medicinal materials based on cryogenic pulverization in this solution, in the pre-freezing stage: place Panax notoginseng in a biological low-temperature refrigerator for pre-freezing, from 0°C to -20°C, for 10 - 20 minutes; in the deep-freezing stage: from -20°C to -40°C, cool at a rate of 1 - 2°C / min for 20 - 30 minutes; in the ultra-low temperature stage: from -40°C to -80°C, cool at a rate of 2 - 4°C / min for 10 - 20 minutes. More specifically, in the pre-freezing stage, the temperature is -20°C and the time is 15 minutes; in the deep-freezing stage, from -20°C to -40°C, cool at a rate of 1.5°C / min for 25 minutes; in the ultra-low temperature stage: from -40°C to -80°C, cool at a rate of 3°C / min for 20 minutes. Under these conditions, the cell structure of Panax notoginseng is intact, the retention rate of active ingredients is high, and the pulverized Panax notoginseng powder is fine and non-agglomerated.

[0020] In the present invention, the pulverization speed is one of the important technologies for achieving the technical effects. The pulverization speed, that is, the rotational speed, refers to the rotational speed of the pulverizer blade or rotor. The pulverization speed directly affects the pulverization efficiency, the quality of the powder, and the retention rate of active ingredients. With a high rotational speed, the pulverization efficiency is high, and the target particle size can be achieved in a shorter time, but it may cause the material to heat up, affecting heat-sensitive components (such as saponins, polysaccharides, etc.), and high-speed impact may cause damage to the cell structure and loss of active ingredients. With a low rotational speed, the material heats up less, which is suitable for protecting heat-sensitive components, causes less damage to the cell structure, and has a higher retention rate of active ingredients, but may lead to low pulverization efficiency and require a longer time to reach the target particle size, and the resulting Panax notoginseng powder has uneven particle sizes and is not fully pulverized. Therefore, different materials should select different appropriate pulverization speeds. For materials rich in heat-sensitive components like Panax notoginseng, the first pulverization speed is medium to low speed, 1500 - 2000 rpm, for 5 - 10 minutes, which can balance the pulverization efficiency and retain active ingredients; the second pulverization speed is high speed, 2500 - 3000 rpm, for 10 - 15 minutes, which can perform fine pulverization to ensure the fineness and non-agglomeration of the Panax notoginseng powder, and at the same time further effectively retain active ingredients. Preferably, the first pulverization speed is 1800 rpm and the time is 10 minutes, and the second pulverization speed is 2800 rpm and the time is 15 minutes.

[0021] In a preferred embodiment of the present invention, the above-mentioned method for processing Panax notoginseng medicinal material based on ultra-low temperature pulverization comprises the following steps:

[0022] 1) Preparation stage: Remove the rhizomes, young shoots, fibrous roots and mud from Panax notoginseng, clean it, drain it, chop it into granules while it is fresh, and sieve it;

[0023] 2) Pre-freezing stage: Place the Panax notoginseng in a biological low-temperature refrigerator for pre-freezing at -20°C for 15 minutes;

[0024] 3) Deep cooling stage: -20℃ to -40℃, cooling at a rate of 1.5℃ / min for 25min;

[0025] 4) Ultra-low temperature stage: -40℃ to -80℃, cooling at a rate of 3℃ / min for 20min;

[0026] 5) Crushing stage: maintained at -100°C, the first crushing speed is 1800rpm, the time is 10min, the second crushing speed is 2800rpm, the time is 15min, the powder is collected, sieved through 80-200 mesh, sealed and packaged, and the packaged powder is stored in a low temperature environment below -20°C.

[0027] Compared with the prior art, the present invention has the following outstanding advantages and beneficial effects:

[0028] The Panax notoginseng medicinal material processing method based on ultra-low temperature crushing of the present invention combines the technology of gradient cooling and step-by-step crushing, and is suitable for processing traditional Chinese medicinal materials such as Panax notoginseng that are rich in heat-sensitive active ingredients (such as saponins, polysaccharides, etc.).

[0029] 1) Through gradient cooling, the material temperature is gradually lowered to avoid excessive ice crystals or cell structure damage caused by rapid freezing, and to maximize the protection of heat-sensitive components such as saponins and polysaccharides. The low temperature environment inhibits the activity of enzymes in Panax notoginseng and prevents the active ingredients from being enzymatically hydrolyzed during the crushing process.

[0030] 2) Optimize the particle size by crushing in steps, first do coarse crushing, then fine crushing, control the particle size distribution in stages, improve crushing efficiency, reduce energy consumption, reduce the load of single crushing, and extend the service life of the equipment.

[0031] Staged freezing and pulverizing technology has significant advantages in the processing of Panax notoginseng, including protecting active ingredients, improving pulverizing efficiency, improving powder quality, reducing energy consumption, and improving process controllability. By controlling the temperature and pulverizing parameters in stages, efficient and low-loss Panax notoginseng processing can be achieved. The obtained Panax notoginseng powder can not only be directly used and sold as a commodity, but also can be processed and used as the raw material of Compound Thromboton Capsules, providing technical support for the modernization of traditional Chinese medicine production. BRIEF DESCRIPTION OF THE DRAWINGS

[0032] Figure 1 Appearance diagram of Panax notoginseng powder prepared in Example 1

[0033] Figure 2 Appearance diagram of Panax notoginseng powder prepared in Comparative Example 5 Detailed implementation manners

[0034] The present invention will be further described in detail below in conjunction with the embodiments, but the implementation manners of the invention are not limited thereto.

[0035] The above embodiments are preferred implementation manners of the present invention, but the implementation manners of the present invention are not limited by the above embodiments. Any other changes, modifications, substitutions, combinations, and simplifications made without departing from the spirit and principle of the present invention shall be equivalent replacement manners and are all included in the protection scope of the present invention.

[0036] The present invention uses Haier Biological Low Temperature Refrigerator dw-86L and BCF-450 Ultra Low Temperature Freezing Mill.

[0037] Example 1

[0038] 1) Preparation stage: Remove the rhizome, young buds, fibrous roots and sediment from Panax notoginseng, wash it clean, drain it, cut it into pieces while it is fresh, and sieve it. The aperture of the sieve mesh is 12 mm;

[0039] 2) Pre-freezing stage: Place Panax notoginseng in the biological low temperature refrigerator for pre-freezing at -20°C for 15 minutes to obtain pre-frozen Panax notoginseng;

[0040] 3) Deep cooling stage: From -20°C to -40°C, cool down at a speed of 1.5°C / min for 25 minutes;

[0041] 4) Ultra low temperature stage: From -40°C to -80°C, cool down at a speed of 3°C / min for 20 minutes;

[0042] 5) Crushing stage: Maintain at -100°C, the first crushing speed is 1800 rpm for 10 minutes, the second crushing speed is 2800 rpm for 15 minutes, collect the powder, sieve it through 80 - 200 meshes, seal and package it, store the packaged powder in a low temperature environment below -20°C, and seal and store it using a moisture-proof non-transparent bag.

[0043] Example 2

[0044] 1) Preparation stage: Remove the rhizome, young buds, fibrous roots and sediment from Panax notoginseng, wash it clean, drain it, cut it into pieces while it is fresh, and sieve it. The aperture of the sieve mesh is 12 mm;

[0045] 2) Pre-freezing stage: Place Panax notoginseng in the biological low temperature refrigerator for pre-freezing at 0°C for 10 minutes to obtain pre-frozen Panax notoginseng;

[0046] 3) Deep cooling stage: from -20°C to -40°C, cooling at a rate of 1°C / min for 20 min;

[0047] 4) Ultra-low temperature stage: from -40°C to -80°C, cooling at a rate of 2°C / min for 20 min;

[0048] 5) Crushing stage: maintaining at -80°C, the first crushing speed is 1500 rpm for 5 min, the second crushing speed is 2500 rpm for 10 min, collecting the powder, screening through 80 - 200 mesh, sealing and packaging, storing the packaged powder in a low-temperature environment below -20°C, and storing it sealed with a moisture-proof non-transparent bag.

[0049] Example 3

[0050] 1) Preparation stage: Remove the rhizome, young buds, fibrous roots and sediment from Panax notoginseng, wash it clean, drain it, cut it into pieces while it is fresh, screen it, and the screen aperture is 12 mm;

[0051] 2) Pre-freezing stage: Place Panax notoginseng in a biological low-temperature refrigerator for pre-freezing at -20°C for 20 min to obtain pre-frozen Panax notoginseng;

[0052] 3) Deep cooling stage: from -20°C to -40°C, cooling at a rate of 2°C / min for 30 min;

[0053] 4) Ultra-low temperature stage: from -40°C to -80°C, cooling at a rate of 4°C / min for 10 min;

[0054] 5) Crushing stage: maintaining at -100°C, the first crushing speed is 2000 rpm for 10 min, the second crushing speed is 3000 rpm for 15 min, collecting the powder, screening through 80 - 200 mesh, sealing and packaging, storing the packaged powder in a low-temperature environment below -20°C, and storing it sealed with a moisture-proof non-transparent bag.

[0055] Example 4

[0056] 1) Preparation stage: Remove the rhizome, young buds, fibrous roots and sediment from Panax notoginseng, wash it clean, drain it, cut it into pieces while it is fresh, screen it, and the screen aperture is 12 mm;

[0057] 2) Pre-freezing stage: Place Panax notoginseng in a biological low-temperature refrigerator for pre-freezing at -10°C for 15 min to obtain pre-frozen Panax notoginseng;

[0058] 3) Deep cooling stage: from -20°C to -40°C, cooling at a rate of 1.5°C / min for 25 min;

[0059] 4) Ultra-low temperature stage: from -40°C to -80°C, cooling at a rate of 3.5°C / min for 15 min;

[0060] 5) Crushing stage: maintaining at -90°C, the first crushing speed is 1700 rpm for 8 min, the second crushing speed is 2700 rpm for 12 min, collecting the powder, screening through 80 - 200 mesh, sealing and packaging, storing the packaged powder in a low-temperature environment below -20°C, and storing it sealed with a moisture-proof non-transparent bag.

[0061] Example 5

[0062] 1) Preparation stage: Remove the rhizomes, young buds, fibrous roots and sediment from the pseudo-ginseng, wash it clean, drain it, cut it into granules while it is still fresh, sieve it, sieve mesh aperture: 12 mm;

[0063] 2) Pre-freezing stage: Place the pseudo-ginseng in a biological low-temperature refrigerator for pre-freezing at -5°C for 20 min to obtain pre-frozen pseudo-ginseng;

[0064] 3) Deep cooling stage: from -20°C to -40°C, cooling at a rate of 2°C / min for 20 min;

[0065] 4) Ultra-low temperature stage: from -40°C to -80°C, cooling at a rate of 2.5°C / min for 20 min;

[0066] 5) Crushing stage: maintaining at -100°C, the first crushing speed is 1900 rpm for 6 min, the second crushing speed is 2900 rpm for 14 min, collecting the powder, screening through 80 - 200 mesh, sealing and packaging, storing the packaged powder in a low-temperature environment below -20°C, and storing it sealed with a moisture-proof non-transparent bag.

[0067] Comparative Example 1

[0068] 1) Preparation stage: Remove the rhizomes, young buds, fibrous roots and sediment from the pseudo-ginseng, wash it clean, drain it, cut it into granules while it is still fresh, sieve it, sieve mesh aperture: 12 mm;

[0069] 2) Pre-freezing stage: Place the pseudo-ginseng in a biological low-temperature refrigerator for pre-freezing at 5°C for 5 min to obtain pre-frozen pseudo-ginseng;

[0070] 3) Deep cooling stage: from -10°C to -20°C, cooling at a rate of 0.5°C / min for 20 min;

[0071] 4) Ultra-low temperature stage: from -20°C to -40°C, cooling at a rate of 1°C / min for 20 min;

[0072] 5) Crushing stage: Maintain at -60°C, the first crushing speed is 1000 rpm, the time is 4 min, the second crushing speed is 2000 rpm, the time is 8 min, collect the powder, sieve it through 80 - 200 mesh, seal and package, store the packaged powder in a low-temperature environment below -20°C, and store it sealed with a moisture-proof opaque bag.

[0073] Comparative Example 2

[0074] 1) Preparation stage: Remove the rhizome, young buds, fibrous roots and sediment from Panax notoginseng, wash it clean, drain it, cut it into granules while it is fresh, sieve it, sieve mesh aperture: 12 mm;

[0075] 2) Pre-freezing stage: Place Panax notoginseng in a biological low-temperature refrigerator for pre-freezing at -25°C for 25 min to obtain pre-frozen Panax notoginseng;

[0076] 3) Deep-freezing stage: From -40°C to -80°C, cool down at a speed of 5°C / min for 40 min;

[0077] 4) Ultra-low temperature stage: From -80°C to -120°C, cool down at a speed of 2.5°C / min for 20 min;

[0078] 5) Crushing stage: Maintain at -100°C, the first crushing speed is 2500 rpm, the time is 15 min, the second crushing speed is 3500 rpm, the time is 18 min, collect the powder, sieve it through 80 - 200 mesh, seal and package, store the packaged powder in a low-temperature environment below -20°C, and store it sealed with a moisture-proof opaque bag.

[0079] Comparative Example 3

[0080] Delete the pre-freezing stage, and the remaining steps are the same as in Example 1.

[0081] Comparative Example 4

[0082] Delete the deep-freezing stage and the ultra-low temperature stage, and the remaining steps are the same as in Example 1, that is, directly perform cryogenic crushing after pre-freezing Panax notoginseng.

[0083] Comparative Example 5

[0084] 1) Preparation stage: Remove the rhizome, young buds, fibrous roots and sediment from Panax notoginseng, wash it clean, drain it, cut it into granules while it is fresh, sieve it, sieve mesh aperture: 12 mm;

[0085] 2) Pre-freezing stage: Place Panax notoginseng in a biological low-temperature refrigerator for pre-freezing at -20°C for 15 min to obtain pre-frozen Panax notoginseng;

[0086] 3) Deep-freezing stage: From -20°C to -40°C, cool down at a speed of 1.5°C / min for 25 min;

[0087] 4) Ultra-low temperature stage: from -40°C to -80°C, cooling at a rate of 3°C / min for 20 min;

[0088] 5) Crushing stage: maintaining at -100°C, crushing speed of 2800 rpm, for 25 min, collecting the powder, sieving through 80 - 200 meshes, sealing and packaging, storing the packaged powder in a low-temperature environment below -20°C, and sealing and storing it with a moisture-proof non-transparent bag.

[0089] Example 6

[0090] Carry out relevant comparative tests on Examples 1 to 5 and Comparative Examples 1 to 5 as follows:

[0091] 1. Determination of the contents of ginsenoside Rg1, ginsenoside Rb1, and notoginsenoside R1: Determine the contents of ginsenoside Rg1, ginsenoside Rg1, and notoginsenoside R1 according to the method specified in the determination item of notoginseng in Part I of the Pharmacopoeia of the People's Republic of China (2020 Edition).

[0092] 2. Comparison of appearance properties

[0093] Table 1 Comparison table of the contents and appearance properties of notoginseng medicinal materials prepared under different crushing conditions

[0094]

[0095]

[0096] As can be seen from the data in Table 1, during the cryogenic grinding process of Panax notoginseng, stepwise freezing, the cooling rate, and the grinding rate have a significant impact on the content of Panax notoginseng. In Comparative Example 1, the stepwise freezing temperature is relatively high, the cooling rate is slow, and the grinding rate is slow. Larger ice crystals are formed in the cells due to the slow cooling, resulting in the destruction of the cell structure, causing the saponin components to exude from the ruptured cells and reducing the retention rate. Therefore, the contents of ginsenoside Rg1, ginsenoside Rb1, and notoginsenoside R1 are relatively low. At the same time, the Panax notoginseng powder particles are relatively rough. In Comparative Example 2, the stepwise freezing temperature is relatively low, the cooling rate is fast, and the freezing time is long, and the grinding rate is fast. Although the contents of ginsenoside Rg1, ginsenoside Rb1, and notoginsenoside R1 are higher than those in Comparative Example 1, the energy consumption also increases. And due to the too-fast grinding rate, the contents of ginsenoside Rg1, ginsenoside Rb1, and notoginsenoside R1 in Panax notoginseng are actually lower than those in Examples 1-5. In Comparative Example 3, pre-freezing is not adopted, and ice crystals are formed inside and outside the Panax notoginseng cells, resulting in the destruction of the cell structure, and the saponin components are lost due to cell rupture. Therefore, the contents of ginsenoside Rg1, ginsenoside Rb1, and notoginsenoside R1 in Panax notoginseng are relatively low. In Comparative Example 4, gradient cooling is not adopted, and the temperature is rapidly reduced to ultra-low temperature. The water in the cells may crystallize rapidly, resulting in potential damage to the cell structure. Therefore, the contents of ginsenoside Rg1, ginsenoside Rb1, and notoginsenoside R1 in Panax notoginseng are relatively low. In Comparative Example 5, stepwise grinding is not adopted. Therefore, the obtained Panax notoginseng powder particles are rough, not delicate, and dark yellow in color. In Examples 1-5, all parameters fall within the scope of the present invention. The methods of stepwise freezing, gradient cooling, and stepwise grinding are used. And in Example 1, various operations, including temperature, time, and speed, are all the most preferred. The overall combination of the above conditions improves the contents of ginsenoside Rg1, ginsenoside Rb1, and notoginsenoside R1 in the Panax notoginseng powder. And the obtained Panax notoginseng powder particles are rough, dry, uniform in color, natural in smell, and easy to dissolve, meeting the market requirements.

[0097] Preparation of Compound Xueshuantong Capsules in Example 7

[0098] Using the Panax notoginseng powder prepared in Example 1, referring to the preparation method of Compound Xueshuantong Capsules in the Chinese Pharmacopoeia (2020 Edition), Compound Xueshuantong Capsules were prepared, and the content determination method of Compound Xueshuantong Capsules in the Chinese Pharmacopoeia (2020 Edition) was used to determine the Panax notoginseng components.

[0099] Table 2 Content Table of Active Components of Panax notoginseng in Compound Xueshuantong Capsules

[0100]

[0101] It can be seen from the data in Table 2 that the Panax notoginseng powder prepared in Example 1 is used to prepare the Compound Thrombolytic Encapsulation Capsules, and the active ingredients ginsenoside Rg1, ginsenoside Rb1, ginsenoside Re and notoginsenoside R1 are significantly improved, and each Compound Thrombolytic Encapsulation Capsule contains a total amount of ginsenoside Rg1, ginsenoside Rb1, ginsenoside Re and notoginsenoside R1 of 12.456 mg, which is higher than the 9.5 mg specified in the 2020 edition of the "Chinese Pharmacopoeia". It can be seen that the Panax notoginseng powder prepared by the ultra-low temperature crushing method of the present invention can not only be directly used and sold as a high-quality commodity, but also can be processed as the raw material of the Compound Thrombolytic Encapsulation Capsules for medicine, thereby improving the quality of the Compound Thrombolytic Encapsulation Capsules.

[0102] The above embodiments are preferred implementation modes of the present invention, but the implementation modes of the present invention are not limited to the above embodiments. Any other changes, modifications, substitutions, combinations, and simplifications made without departing from the spirit and principles of the present invention shall be equivalent replacement methods and shall be included in the protection scope of the present invention.

Claims

1. A method for processing Panax notoginseng medicinal material based on ultra-low temperature pulverization, characterized in that: It includes the following steps: 1) Preparation stage: Remove the rhizomes, young buds, fibrous roots and sediment from the pseudo-ginseng, wash it clean, drain it, break it into granules while it is fresh, and sieve it; 2) Pre-freezing stage: Place the pseudo-ginseng in a biological low-temperature refrigerator for pre-freezing at 0°C to -20°C for 10 - 20 minutes; 3) Deep-freezing stage: From -20°C to -40°C, cool it at a rate of 1 - 2°C / min for 20 - 30 minutes; 4) Ultra-low temperature stage: From -40°C to -80°C, cool it at a rate of 2 - 4°C / min for 10 - 20 minutes; 5) Crushing stage: Maintain at -80°C to -100°C, the first crushing speed is 1500 - 2000 rpm for 5 - 10 minutes, the second crushing speed is 2500 - 3000 rpm for 10 - 15 minutes, collect the powder, sieve it through 80 - 200 meshes, seal and package it, and store the packaged powder in a low-temperature environment below -20°C.

2. The method according to claim 1, characterized in that, In the pre-freezing stage, the temperature is -20°C and the time is 15 minutes.

3. The method according to claim 1, wherein In the deep-freezing stage, from -20°C to -40°C, cool it at a rate of 1.5°C / min for 25 minutes.

4. The method according to claim 1, wherein In the ultra-low temperature stage, from -40°C to -80°C, cool it at a rate of 3°C / min for 20 minutes.

5. The method according to claim 1, characterized in that, In the crushing stage, the temperature is -100°C.

6. The method according to claim 1, wherein In the crushing stage, the first crushing speed is 1800 rpm for 10 minutes, and the second crushing speed is 2800 rpm for 15 minutes.

7. A method for processing Panax notoginseng medicinal material based on ultra-low temperature pulverization, characterized in that: It includes the following steps: 1) Preparation stage: Remove the rhizomes, young buds, fibrous roots and sediment from the pseudo-ginseng, wash it clean, drain it, cut it into granules while it is fresh, and sieve it; 2) Pre-freezing stage: Place the pseudo-ginseng in a biological low-temperature refrigerator for pre-freezing at -20°C for 15 minutes; 3) Deep-freezing stage: From -20°C to -40°C, cool it at a rate of 1.5°C / min for 25 minutes; 4) Ultra-low temperature stage: From -40°C to -80°C, cool it at a rate of 3°C / min for 20 minutes; 5) Crushing stage: Maintain at -100°C, the first crushing speed is 1800 rpm for 10 minutes, the second crushing speed is 2800 rpm for 15 minutes, collect the powder, sieve it through 80 - 200 meshes, seal and package it, and store the packaged powder in a low-temperature environment below -20°C.

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