Kit for cell detection

Abstract

The invention provides a kit for cell detection, and relates to the technical field of kits. The kit for cell detection comprises a kit main body, the bottom of the inner surface of the kit main body is fixedly connected with a bottom plate, the top of the bottom plate is provided with a vibration assembly, and the movable end of the vibration assembly is fixedly connected with a detection tube placing cylinder; a supporting column is fixedly connected to one side of the top of the bottom plate, and an electric rotating frame is installed at the top end of the supporting column. Through cooperative control of the electric rotating frame, the lifting assembly and the sealing assembly, whole-process automation of reagent adding is achieved. Compared with the operation of manually taking the reagent bottle and manually installing the dropper in the prior art, according to the scheme, the cylindrical shell is driven by the motor to rotate and position, the piston assembly is accurately pressed down, and the sealing plate is automatically opened and closed, so that low efficiency and error risks caused by manual operation are thoroughly avoided, and the standardization and reliability of the detection process are remarkably improved.

Description

Technical Field

[0001] The present invention relates to the technical field of reagent kits, and specifically to a reagent kit for cell detection. Background Art

[0002] A reagent kit is a laboratory or medical testing tool mainly used to hold chemical reagents required for detecting specific chemical components, drug residues, virus species, etc., and provides a standardized operation process. Purified proteins are the core raw materials or reference standards for many cell detection reagent kits. Reagent kits for cell detection are widely used in hospitals, pharmaceutical companies, and research laboratories, involving disease diagnosis (such as COVID-19 virus detection), drug research and development, food safety monitoring, etc.

[0003] A prior art tumor cell detection reagent kit and its detection method with the publication number CN119186327B includes a reagent kit body. An inner box frame is arranged inside the reagent kit body. A round hole is opened at the top of the inner box frame, and a detection uniformity module is arranged inside the round hole. A fixed frame is fixedly connected to the inner wall of the top of the inner box frame.

[0004] This reagent kit increases the diversity of the use of the reagent kit body, thus facilitating the device for detection use, and reduces the auxiliary equipment required when the device is used, further increasing the use effect of the device. However, its degree of automation still has obvious limitations. Key operation links such as reagent extraction still require manual handling of reagent bottles, and the installation of droppers also depends on manual rotation adjustment, which forms a sharp contrast with the complex mechanical structure and motor drive system of the reagent kit. This partial automation design leads to the contradiction between high manufacturing costs and the fact that more manual intervention is still required in actual operation, making the functional improvement brought by the complex structure not fully match the expected effect. Especially, the switching and positioning of droppers still require manual operation, which not only reduces the detection efficiency but also increases the risk of operation errors, and the overall auxiliary effect fails to fully exert the advantages that its structural design should have.

[0005] Therefore, we have developed a new reagent kit for cell detection. Summary of the Invention

[0006] (1) Technical Problems to be Solved

[0007] In view of the deficiencies of the prior art, the present invention provides a reagent kit for cell detection, which solves the problems that key operation links of the existing cell detection reagent kits still rely on manual intervention, resulting in the mismatch between its complex automation structure and actual use effect, and insufficient cost performance.

[0008] (2) Technical Solutions

[0009] To achieve the above object, the present invention is realized through the following technical solutions: A kit for cell detection, comprising a box body, the bottom of the inner surface of the box body is fixedly connected with a bottom plate, a vibration assembly is installed on the top of the bottom plate, and a detection tube placement cylinder is fixedly connected to the movable end of the vibration assembly;

[0010] One side of the top of the bottom plate is fixedly connected with a support column, the top of the support column is installed with an electric rotating frame, and a plurality of cylindrical shells are fixedly connected to the movable end of the electric rotating frame;

[0011] One side of the outer surface of a plurality of the cylindrical shells is detachably and fixedly connected with reagent cylinders;

[0012] A sealing assembly is jointly opened between the bottoms of a plurality of the reagent cylinders and the adjacent cylindrical shells;

[0013] Piston assemblies are sealed inside a plurality of the reagent cylinders, and a lifting assembly is jointly installed between a plurality of the piston assemblies and the adjacent cylindrical shells.

[0014] Preferably, the top of the box body is sealed and buckled with a box cover.

[0015] Through the above technical solution, the box cover and the box body adopt a sealed buckling design, which can effectively prevent external pollutants from entering the box, and at the same time maintain a sterile environment inside the kit, which is particularly suitable for cell detection scenarios with high cleanliness requirements.

[0016] Preferably, the vibration assembly includes a housing, and the housing is fixedly installed on the top of the bottom plate. The inner walls of the four sides of the housing are all fixedly connected with tension springs. The four tension springs are jointly fixedly connected with a movable plate, and the detection tube placement cylinder is fixedly installed on the top of the movable plate. A square hole penetrating to the bottom is opened on the top of the movable plate, and a cam is attached to the inner wall of one side of the square hole. One side of the top of the housing is fixedly installed with a first motor, and the drive shaft of the first motor penetrates through the housing and is fixedly connected with the cam.

[0017] Through the above technical solution, the vibration assembly adopts a cam-spring structure. The cam is driven by the first motor to rotate, driving the movable plate to reciprocate, so that the detection tube placement cylinder generates high-frequency vibration. This design can not only achieve rapid and uniform mixing of the reagent, but also avoid liquid splashing or bubble generation caused by traditional manual shaking, improving the stability and repeatability of detection.

[0018] Preferably, a through hole penetrating to the inside is opened on the top of the housing, and the through hole matches the detection tube placement cylinder.

[0019] Through the above technical solution, the design of the through hole enables the detection tube placement cylinder to vibrate freely inside the housing.

[0020] Preferably, a test tube is placed and inserted into the test tube placement cylinder in a limited manner.

[0021] Through the above technical solution, the test tube is fixed in the placement cylinder by limited insertion, ensuring that it will not loosen or shift during vibration, and at the same time facilitating quick installation and replacement. This design simplifies the operation process, improves the detection efficiency, and is especially suitable for batch sample processing.

[0022] Preferably, the electric rotating frame includes a second motor, and the second motor is fixedly installed on one side of the support column. The driving end of the second motor is fixedly connected with a driving gear, and the outer surface of the driving gear is meshed with a driven gear. The inner surface of the driven gear is fixedly connected with a movable frame, and the movable frame is rotatably sleeved on the top of the support column and fixedly connected with a plurality of cylindrical shells.

[0023] Through the above technical solution, the electric rotating frame adopts a gear transmission structure. The second motor drives the driving gear to drive the driven gear to rotate, so as to realize the precise positioning of a plurality of cylindrical shells. This design not only improves the automation degree of reagent addition, but also ensures the quick switching of different reagent cylinders, avoiding errors that may be brought by manual operation.

[0024] Preferably, a positioning seat is fixedly connected to one side of the outer surface of each of the plurality of cylindrical shells. A positioning block is inserted into the inner side of each of the plurality of positioning seats in a positioned manner. The plurality of positioning blocks are all fixedly connected with the positioning seats through hand-tightening bolts, and the positioning blocks are fixedly connected with the reagent cylinders.

[0025] Through the above technical solution, this modular design improves the flexibility and applicability of the reagent kit, and at the same time reduces the maintenance cost.

[0026] Preferably, each of the plurality of closing assemblies includes a third motor, and the third motor is fixedly installed on the inner top of the cylindrical shell. The driving shaft of the third motor penetrates through the cylindrical shell and is fixedly connected with a sealing plate, and the sealing plate is in sealing fit with the bottom of the reagent cylinder.

[0027] Through the above technical solution, the closing assembly drives the sealing plate to rotate through the third motor to realize the automatic opening and closing of the bottom of the reagent cylinder. This design can not only accurately control the release timing of the reagent, but also avoid reagent leakage or contamination, ensuring the cleanliness and accuracy of the detection process.

[0028] Preferably, each of the plurality of piston assemblies includes a piston body, and the piston body is in sealing fit with the inside of the reagent cylinder. A piston rod is fixedly connected to the top of the piston body.

[0029] Through the above technical solution, the piston assembly ensures that there is no residue or leakage of the reagent during the pushing process through the close fit of the piston body with the inner wall of the reagent cylinder. This design can accurately control the addition amount of the reagent, improving the repeatability of the detection and the reliability of the data.

[0030] Preferably, each of the plurality of lifting components includes a cavity, and the cavity is opened at the inner top of the cylindrical shell. A travel hole penetrating to the outside is opened on one side of the inner surface of the cavity.

[0031] Each of the lifting components further includes a fourth motor, and the fourth motor is fixedly installed inside the cylindrical shell. A screw rod is fixedly connected to the drive shaft of the fourth motor, and the screw rod penetrates into the cavity and is rotatably connected to the cylindrical shell. A sliding seat is threadedly sleeved on the outer surface of the screw rod, and the sliding seat is in sliding fit with the inner walls on both sides of the travel hole and is fixedly connected to the piston rod.

[0032] Through the above technical solution, the lifting component adopts a screw rod and sliding seat transmission structure. The fourth motor drives the screw rod to rotate, driving the sliding seat to move up and down along the travel hole, thereby accurately controlling the lifting of the piston rod. This design can realize the quantitative addition of reagents, avoid the dosage errors that may be brought by manual operation, and improve the accuracy and efficiency of detection.

[0033] (III) Advantageous Effects

[0034] The present invention provides a kit for cell detection, which has the following advantageous effects:

[0035] 1. For the kit for cell detection, through the coordinated control of the electric rotating frame, the lifting component and the closing component, the whole process of reagent addition is automated. Compared with the prior art where reagent bottles still need to be manually taken and manual droppers need to be installed, in this solution, the cylindrical shell is driven by a motor to rotate and position, the piston assembly is accurately pressed down, and the sealing plate is automatically opened and closed, completely avoiding the low efficiency and error risks brought by manual operation, and significantly improving the standardization and reliability of the detection process.

[0036] 2. For the kit for cell detection, the reagent cylinder is detachably fixed to the cylindrical shell through the positioning block and the hand-tightening bolt, supporting rapid replenishment of consumables. This design can flexibly configure the number of reagents according to detection requirements, and is also convenient for maintenance and cleaning, extending the service life of the equipment;

[0037] 3. For the kit for cell detection, through the cooperation of the cam and the tension spring in the vibration component, the detection tube placement cylinder is driven to vibrate at a high frequency, so that the blood in the detection test tube is fully mixed with the reagent. Compared with manual shaking or static reaction, this mechanical vibration ensures the uniformity and stability of the reaction system, especially suitable for cell detection scenarios with high requirements for mixing accuracy, thereby improving the accuracy of the detection results. BRIEF DESCRIPTION OF THE DRAWINGS

[0038] Figure 1 is a perspective view of the present invention;

[0039] Figure 2 is a perspective view of the present invention with the lid removed;

[0040] Figure 3 Front view of the main box body and the box cover of the present invention removed;

[0041] Figure 4 Second view of the main box body and the box cover of the present invention removed;

[0042] Figure 5 First view of the vibration assembly of the present invention;

[0043] Figure 6 Second view of the vibration assembly of the present invention;

[0044] Figure 7 Cross-sectional view of the cylindrical shell of the present invention.

[0045] Wherein, 1, box main body; 2, box cover; 3, test tube; 4, movable rack; 5, cylindrical shell; 6, test tube placement cylinder; 7, driven gear; 8, bottom plate; 9, support column; 10, second motor; 11, hand-tightening bolt; 12, driving gear; 13, outer shell; 14, reagent cylinder; 15, tension spring; 16, square hole; 17, cam; 18, movable plate; 19, first motor; 20, through hole; 21, sealing plate; 22, third motor; 23, positioning block; 24, positioning seat; 25, fourth motor; 26, piston body; 27, piston rod; 28, stroke hole; 29, sliding seat; 30, cavity; 31, screw. Detailed implementation manners

[0046] Next, the technical solutions in the embodiments of the present invention will be clearly and completely described in conjunction with the accompanying drawings in the embodiments of the present invention. Obviously, the described embodiments are only a part of the embodiments of the present invention, rather than all the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by those of ordinary skill in the art without creative efforts shall fall within the protection scope of the present invention.

[0047] As Figure 1 shown, an embodiment of the present invention provides a cell detection kit, including a box main body 1, and a box cover 2 is hermetically buckled on the top of the box main body 1. This hermetic buckling structure adopts a rubber sealing ring and a buckle design, which can not only ensure the sealing performance but also facilitate the opening and closing operation, ensuring that the internal reagents are not contaminated by the outside.

[0048] Figures 2 - 6As shown in the figure, a bottom plate 8 is fixedly connected to the bottom of the inner surface of the box body 1. A vibration assembly is installed on the top of the bottom plate 8. The movable end of the vibration assembly is fixedly connected to a test tube placement cylinder 6. The vibration assembly includes a housing 13, and the housing 13 is fixedly installed on the top of the bottom plate 8. Pulling springs 15 are fixedly connected to the inner walls of the four sides of the housing 13. The four pulling springs 15 are jointly fixedly connected to a movable plate 18, and the test tube placement cylinder 6 is fixedly installed on the top of the movable plate 18. A square hole 16 penetrating through to the bottom is opened on the top of the movable plate 18. A cam 17 is attached to one inner wall of the square hole 16. A first motor 19 is fixedly installed on one side of the top of the housing 13, and the driving shaft of the first motor 19 penetrates through the housing 13 and is fixedly connected to the cam 17. A through hole 20 penetrating through to the inside is opened on the top of the housing 13, and the through hole 20 matches the test tube placement cylinder 6. A test tube 3 is inserted into the test tube placement cylinder 6 in a limited way. A rubber sleeve is arranged in the test tube placement cylinder 6, and the rubber sleeve can squeeze and fix the test tube 3.

[0049] Figures 3 - 4 As shown in the figure, a support column 9 is fixedly connected to one side of the top of the bottom plate 8. An electric rotating frame is installed at the top end of the support column 9. The movable end of the electric rotating frame is fixedly connected to a plurality of cylindrical shells 5. The electric rotating frame includes a second motor 10, and the second motor 10 is fixedly installed on one side of the support column 9. The driving end of the second motor 10 is fixedly connected to a driving gear 12. A driven gear 7 is meshed on the outer surface of the driving gear 12. The inner surface of the driven gear 7 is fixedly connected to a movable frame 4, and the movable frame 4 is rotatably sleeved on the top end of the support column 9 and is fixedly connected to the plurality of cylindrical shells 5. The gear transmission mechanism adopts a precision reduction design, and the positioning accuracy of ±0.5° can be achieved to ensure the accuracy of reagent addition.

[0050] Figure 3 、 Figure 7 As shown in the figure, reagent tubes 14 are detachably fixedly connected to one side of the outer surfaces of the plurality of cylindrical shells 5. Positioning seats 24 are fixedly connected to one side of the outer surfaces of the plurality of cylindrical shells 5. Positioning blocks 23 are inserted into the inner sides of the plurality of positioning seats 24 in a positioned way. The plurality of positioning blocks 23 are fixedly connected to the positioning seats 24 through hand-tightening bolts 11, and the positioning blocks 23 are fixedly connected to the reagent tubes 14. The thread specification of the hand-tightening bolts 11 is M4, which not only ensures the connection strength but also is convenient for manual operation.

[0051] Figure 7 As shown in the figure, a closing assembly is jointly opened between the bottoms of the plurality of reagent tubes 14 and the adjacent cylindrical shells 5. The plurality of closing assemblies each include a third motor 22, and the third motor 22 is fixedly installed on the inner top of the cylindrical shell 5. The driving shaft of the third motor 22 penetrates through the cylindrical shell 5 and is fixedly connected to a sealing plate 21, and the sealing plate 21 is sealingly attached to the bottom of the reagent tube 14. The sealing plate 21 is made of polytetrafluoroethylene material, forming a surface seal with the bottom of the reagent tube 14, and the sealing pressure can reach 0.3 MPa to ensure no leakage.

[0052] Figure 7 As shown, piston assemblies are sealed inside multiple reagent cylinders 14. Lifting assemblies are commonly installed between multiple piston assemblies and the adjacent cylindrical shells 5. Multiple piston assemblies each include a piston body 26, and the piston body 26 is hermetically fitted inside the reagent cylinder 14. A piston rod 27 is fixedly connected to the top of the piston body 26. Multiple lifting assemblies each include a cavity 30, and the cavity 30 is opened at the inner top of the cylindrical shell 5. A stroke hole 28 penetrating to the outside is opened on one side of the inner surface of the cavity 30. The lifting assemblies also each include a fourth motor 25, and the fourth motor 25 is fixedly installed inside the cylindrical shell 5. A screw rod 31 is fixedly connected to the drive shaft of the fourth motor 25, and the screw rod 31 penetrates into the cavity 30 and is rotatably connected to the cylindrical shell 5. A sliding seat 29 is threadedly sleeved on the outer surface of the screw rod 31, and the sliding seat 29 is in sliding fit with the inner walls on both sides of the stroke hole 28 and is fixedly connected to the piston rod 27. The screw rod 31 is a precision ball screw with a lead of 2 mm. With the step control of the fourth motor 25, a reagent addition accuracy of 0.01 ml can be achieved.

[0053] Working principle: During actual use, a controller should be installed to control the overall operation.

[0054] Step 1. Initial preparation stage

[0055] Open the lid 2: The user opens the sealed lid 2 to expose the internal structure of the box body 1.

[0056] Place the test tube 3 for detection: Insert the test tube 3 containing the patient's blood into the test tube placement cylinder 6, and fix the position of the test tube through the limiting structure inside the cylinder to ensure stability.

[0057] Step 2. Reagent addition stage

[0058] Start the electric rotating frame: Drive the driving gear 12 and the driven gear 7 through the second motor 10 to drive the movable frame 4 and the cylindrical shell 5 to rotate, so that the target reagent cylinder 14 is aligned above the test tube 3 for detection.

[0059] Open the closing assembly: The third motor 22 drives the sealing plate 21 to rotate, opens the outlet at the bottom of the reagent cylinder 14, and releases the sealed state.

[0060] Press down the piston assembly: The fourth motor 25 starts, drives the screw rod 31 to rotate, pushes the sliding seat 29 to move downward along the stroke hole 28, presses down the piston body 26 through the piston rod 27, and precisely squeezes the reagent in the reagent cylinder 14 into the test tube 3 for detection.

[0061] Note: Different reagent cylinders 14 can be aligned with the test tube for detection in sequence by rotation to achieve step-by-step addition of multiple reagents and avoid cross-contamination.

[0062] Step 3: Vibration mixing stage

[0063] The vibration assembly works: The first motor 19 drives the cam 17 to rotate, periodically pressing against the inner wall of the square hole 16 of the movable plate 18. Cooperating with the resilience of the tension spring 15, the movable plate 18 and the test tube placement cylinder 6 generate high-frequency vibrations.

[0064] Uniform mixing: Vibration prompts the blood and reagent in the test tube 3 to disperse and react quickly, improving the detection accuracy.

[0065] Step 4: Reset and cleaning

[0066] Reset the piston and seal: The lifting assembly drives the piston body 26 to reset, and the closing assembly reseals the bottom of the reagent cylinder 14 to prevent the leakage of residual reagent.

[0067] Close the lid 2: After the detection is completed, close the lid 2 to maintain a sterile environment inside.

[0068] Although the embodiments of the present invention have been shown and described, for those of ordinary skill in the art, it can be understood that various changes, modifications, substitutions, and variations can be made to these embodiments without departing from the principle and spirit of the present invention. The scope of the present invention is defined by the appended claims and their equivalents.

Claims

1. A kit for cell detection, comprising a box body (1), characterized in that: A bottom plate (8) is fixedly connected to the bottom of the inner surface of the box body (1). A vibration assembly is installed on the top of the bottom plate (8), and a detection tube placement cylinder (6) is fixedly connected to the movable end of the vibration assembly; A support column (9) is fixedly connected to one side of the top of the bottom plate (8). The top of the support column (9) is equipped with an electric rotating frame, and a plurality of cylindrical shells (5) are fixedly connected to the movable end of the electric rotating frame; One side of the outer surface of each of the plurality of cylindrical shells (5) is detachably and fixedly connected with a reagent tube (14); A closed assembly is jointly formed between the bottom of each of the plurality of reagent tubes (14) and the adjacent cylindrical shell (5); A piston assembly is sealed inside each of the plurality of reagent tubes (14), and a lifting assembly is jointly installed between each of the plurality of piston assemblies and the adjacent cylindrical shell (5).

2. The kit for cell detection according to claim 1, characterized in that: A box cover (2) is hermetically buckled on the top of the box body (1).

3. The cell detection kit according to claim 1, wherein: The vibration assembly includes a housing (13), and the housing (13) is fixedly installed on the top of the bottom plate (8). Pull springs (15) are fixedly connected to the inner walls of the four sides of the housing (13). The four pull springs (15) are jointly fixedly connected to a movable plate (18), and the detection tube placement cylinder (6) is fixedly installed on the top of the movable plate (18). A square hole (16) penetrating through to the bottom is formed in the top of the movable plate (18). A cam (17) is attached to one side inner wall of the square hole (16). A first motor (19) is fixedly installed on one side of the top of the housing (13), and the driving shaft of the first motor (19) penetrates through the housing (13) and is fixedly connected to the cam (17).

4. A kit for cell detection according to claim 3, characterized in that: A through hole (20) penetrating through to the inside is formed in the top of the housing (13), and the through hole (20) matches the detection tube placement cylinder (6).

5. A kit for cell detection according to claim 1, characterized in that: A detection test tube (3) is inserted into the detection tube placement cylinder (6) in a limited manner.

6. The kit for cell detection according to claim 1, characterized in that: The electric rotating frame includes a second motor (10), and the second motor (10) is fixedly installed on one side of the support column (9). The driving end of the second motor (10) is fixedly connected to a driving gear (12). The outer surface of the driving gear (12) is meshed with a driven gear (7). The inner surface of the driven gear (7) is fixedly connected to a movable frame (4), and the movable frame (4) is rotatably sleeved on the top of the support column (9) and is fixedly connected to a plurality of cylindrical shells (5).

7. A kit for cell detection according to claim 1, wherein: One side of the outer surface of each of the plurality of cylindrical shells (5) is fixedly connected with a positioning seat (24). A positioning block (23) is inserted into the inside of each of the plurality of positioning seats (24) in a positioned manner. Each of the plurality of positioning blocks (23) is fixedly connected to the positioning seat (24) through a hand-tightening bolt (11), and the positioning block (23) is fixedly connected to the reagent tube (14).

8. A kit for cell detection according to claim 1, characterized in that: Each of the plurality of closed assemblies includes a third motor (22), and the third motor (22) is fixedly installed on the inner top of the cylindrical shell (5). The driving shaft of the third motor (22) penetrates through the cylindrical shell (5) and is fixedly connected to a sealing plate (21), and the sealing plate (21) is hermetically attached to the bottom of the reagent tube (14).

9. A kit for cell detection according to claim 1, characterized in that: Each of the plurality of piston assemblies includes a piston body (26), and the piston body (26) is hermetically attached to the inside of the reagent cylinder (14). A piston rod (27) is fixedly connected to the top of the piston body (26).

10. A kit for cell detection according to claim 9, characterized in that: Each of the plurality of lifting assemblies includes a cavity (30), and the cavity (30) is opened at the inner top of the cylindrical shell (5). A stroke hole (28) penetrating to the outside is opened on one side of the inner surface of the cavity (30); Each of the lifting assemblies further includes a fourth motor (25), and the fourth motor (25) is fixedly installed inside the cylindrical shell (5). A driving shaft of the fourth motor (25) is fixedly connected to a screw rod (31), and the screw rod (31) penetrates into the cavity (30) and is rotatably connected to the cylindrical shell (5). A sliding seat (29) is threadedly sleeved on the outer surface of the screw rod (31), and the sliding seat (29) is in sliding fit with the inner walls on both sides of the stroke hole (28) and is fixedly connected to the piston rod (27).

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