Microbial culture device with vibration assistance for laboratory

Abstract

The invention provides a vibration-assisted microculture device for a laboratory, which belongs to the field of microculture and comprises an incubator, a liquid adding component, a vibration component, a visual module, a detection module, a controller and the like. Partition plates are arranged in the culture box to form two culture cavities, and the liquid adding assemblies are arranged in the cavities and used for adding culture liquid. The vibration assembly is mounted on a mounting seat in the culture box, a vibration base of the vibration assembly is connected with a vibration platform through an air bag and a telescopic rod, and a culture dish can vibrate; the visual module is located above the culture dish and collects image data, and the detection module and the controller judge the uniformity of the culture solution. According to the device, culture solutions are promoted to be mixed through the vibration assembly, and growth limitation of microorganisms due to uneven nutrition is avoided; the visual module is matched with the detection module, so that problems of the culture solution can be found and adjusted in time, automatic monitoring and regulation of the microbial culture process are realized, and the microbial culture efficiency and success rate are effectively improved.

Description

Technical Field

[0001] The present invention relates to the technical field of microorganism cultivation, and in particular to a laboratory microorganism cultivation device with vibration assistance. Background Art

[0002] Microbial culture is widely used in life science research, pharmaceutical development, food fermentation and environmental management. It can effectively separate and purify microorganisms and study their growth characteristics and metabolic mechanisms. In order to ensure the stability of microbial growth and the reliability of experimental results, and to meet different culture requirements, a microbial culture device is needed.

[0003] During the culture process, in order to provide sufficient nutrients to the microorganisms and maintain a suitable osmotic pressure and pH environment, it is necessary to add culture fluid to the culture dish to ensure that the microorganisms can grow and reproduce in a good environment and achieve the expected culture goals. However, traditional microbial culture devices usually only provide a static culture environment with constant temperature and humidity. During the culture process of microorganisms, when the culture fluid is added to the culture dish, the culture fluid is easily added unevenly, resulting in large differences in the growth rate of microorganisms. The growth of microorganisms in some areas is restricted due to lack of nutrition or accumulation of metabolites, making it difficult to achieve efficient and stable culture. At the same time, there is a lack of effective detection methods for the uniformity of the culture fluid, and it is impossible to determine whether the culture fluid has reached a uniform state, making it difficult to make targeted adjustments according to actual conditions. Summary of the invention

[0004] In view of the above-mentioned problems, in combination with the first aspect of the present invention, an embodiment of the present invention provides a laboratory microorganism cultivation device with vibration assistance, the device comprising: A laboratory microbial culture device with vibration assistance, comprising an incubator and a liquid adding assembly for adding culture solution, wherein the incubator is provided with a partition to form two groups of culture chambers, the liquid adding assembly is located in the culture chamber, the incubator is provided with a plurality of mounting seats, the culture chamber is provided with a plurality of vibration assemblies, and the vibration assemblies are mounted on the mounting seats; The vibration assembly includes a vibration base and a vibration platform, the vibration base is provided with a support plate, the vibration platform is located above the support plate, and the vibration platform and the support plate are connected by multiple groups of air bags and multiple groups of telescopic rods; The vibration platform is provided with an illumination light board, and the culture dish is placed on the vibration platform and above the illumination light board; A plurality of visual modules are arranged in the culture chamber, and the visual modules are located above the culture dish. A detection module and a controller for judging the uniformity of the culture liquid are arranged on the culture box, and the detection module is electrically connected to the visual module.

[0005] According to a preferred embodiment, the vibration assembly further includes multiple groups of air pumps. The air pumps are installed on the vibration base and connected to the airbag through connecting pipes. The multiple groups of airbags and the multiple groups of telescopic rods are arranged diagonally; A connecting rod is provided on the support plate. One ends of the connecting rod and the telescopic rod are both spherical. A first connecting block and multiple groups of second connecting blocks are provided at the bottom of the vibration platform. One end of the connecting rod is clamped in the first connecting block, and the vibration platform rotates around the connecting rod. One end of the telescopic rod is clamped in the second connecting block; Two groups of arc-shaped slide rails are provided on the support plate. The two sides of the two groups of arc-shaped slide rails are in contact with each other to form a circular slideway. Electric sliders are slidably arranged on the arc-shaped slide rails, and the electric sliders move back and forth along the circular slideway; An electromagnet is provided on the electric slider, and a magnetic ring is provided at the bottom of the vibration platform.

[0006] According to a preferred embodiment, the liquid adding assembly includes multiple groups of electric cylinders and multiple groups of electric lifting rods. Multiple groups of installation grooves are formed in the incubator, and the multiple groups of installation grooves are respectively located in the two culture cavities. The electric cylinders are installed in the installation grooves, and the electric cylinders are provided with mounting plates through bearings. The electric lifting rods are installed on the mounting plates and are located above the culture dishes; An adsorption disc is provided below the mounting plate. The shaft end of the electric lifting rod passes through the mounting plate and is connected to the adsorption disc. Multiple groups of connecting sleeves are provided at the bottom of the adsorption disc, and suction cups are provided at the bottom of the connecting sleeves. The multiple groups of suction cups are interconnected through a ventilation pipe. Multiple groups of negative pressure generators are provided in the culture cavity, and the negative pressure generators are communicated with the ventilation pipe.

[0007] According to a preferred embodiment, the liquid adding assembly further includes multiple groups of rotating brackets and multiple groups of liquid adding members. The multiple groups of rotating brackets are respectively located in the culture cavity and are installed on the inner wall of the incubator. One end of the rotating bracket is rotatably provided with a support rod, and a liquid adding sleeve is provided at one end of the support rod; The liquid adding member is provided with a liquid inlet pipe and multiple groups of liquid outlet pipes. A bearing is provided in the liquid adding sleeve, and the liquid inlet pipe is arranged through the bearing. The liquid adding member is rotatably connected to the liquid adding sleeve. A liquid supply part is provided on one side of the incubator, and the liquid adding sleeve is connected to the liquid supply part through a liquid supply pipe. The liquid supply pipe is interconnected with the liquid adding sleeve and the liquid adding member.

[0008] According to a preferred embodiment, a rotating box is provided on one side of the liquid adding sleeve, a bearing is provided on the rotating box, a first gear is provided below the rotating box, a rotating rod is provided on the first gear, the rotating rod is inserted into the bearing, the first gear is rotatably connected to the rotating box, a second gear is provided on the liquid adding member, and the first gear meshes with the second gear; A fan blade is provided in the rotating box, the fan blade is sleeved on the rotating rod, an air pipe is provided above the rotating box, the air outlet end of the air pipe faces the fan blade, a fan is provided in the culture cavity, and the air inlet end of the air pipe is connected to the fan.

[0009] According to a preferred embodiment, an assembly seat is provided on one side of the incubator, a humidifying component is provided on the assembly seat, the humidifying component includes an installation table and an evaporation box, the installation table is connected to the assembly seat, a support frame is provided on the installation table, and the evaporation box is installed on the installation table and is located between the installation table and the support frame; A water tank and a water pump are provided on one side of the incubator, the water pump is connected to the water tank and the evaporation box respectively through water pipes, and multiple heating modules and steam generators are provided in the evaporation box; A cooling box is provided above the support frame, multiple partition plates are provided in the cooling box, the cooling box forms multiple gas channels through the multiple partition plates, and the cooling box communicates with the evaporation box through a delivery pipe; Cooling boxes filled with coolant are provided on both sides of the cooling box, a collection box is provided in the cooling box, multiple guide ribs are provided on the inner walls of the gas channels, the length of the guide ribs is greater than the length of the partition plates, and the collection box is located below the guide ribs; The top of the cooling box communicates with the incubator through multiple delivery pipes.

[0010] According to a preferred embodiment, the judgment of the uniformity of the culture solution by the detection module includes the following steps: S1: Obtain the image data parameters after adding the culture solution, analyze the image data parameters through an image recognition algorithm, and obtain the preliminary uniformity parameters; S2: Preset the uniformity standard parameters, compare the preliminary uniformity parameters with the uniformity standard parameters, and obtain the difference value parameters; S3: Perform iterative search and optimization operations on the difference value parameters based on the cuckoo search algorithm to obtain the accurate evaluation parameters of the current culture solution uniformity; S4: Preset the threshold parameters, and perform a comparison operation based on the accurate evaluation parameters and the threshold parameters; S51: If the accurate evaluation parameter is greater than the threshold parameter, it is determined that the uniformity of the culture medium meets the standard, and then the normal culture program of the device is started to culture the microorganisms in the current state; S52: If the accurate evaluation parameter is less than the threshold parameter, it is determined that the uniformity of the culture medium does not meet the standard, and the operation of uniformizing the culture medium is carried out.

[0011] According to a preferred embodiment, in step S3, the differential value parameter is iteratively searched and optimized based on the cuckoo search algorithm, which specifically includes: Initialize the cuckoo population, and use the differential value parameter as the initial solution space; Set the Levy flight strategy to simulate the process of the cuckoo randomly searching for a new nest, and generate a new solution of the uniformity evaluation parameter in each iteration; According to the preset fitness function, comprehensively calculate the preliminary uniformity parameter, the uniformity standard parameter and the generated new solution to evaluate the fitness of each new solution; Retain the solution with the highest fitness as the next-generation population, and randomly select some nests with low fitness for elimination, which are replaced by the newly generated solutions; Repeat the above steps of Levy flight, fitness evaluation and population update until the preset number of iterations is reached or the convergence condition is met, and output the accurate evaluation parameter of the current uniformity of the culture medium.

[0012] According to a preferred embodiment, the operation of uniformizing the culture medium includes: According to the difference between the accurate evaluation parameter and the threshold parameter, use the fuzzy logic algorithm to calculate the vibration frequency and amplitude adjustment coefficient of the vibration component; Control the air pump to adjust the inflation or deflation frequency of the airbag according to the vibration frequency adjustment coefficient, cooperate with the electric slider and the electromagnet to control the amplitude of the vibration platform, and start the vibration component to perform the primary shaking and stirring of the culture medium in the culture dish; After the primary shaking and stirring is completed, repeat the operations of S1-S4 steps to re-judge the uniformity of the culture medium; If the uniformity of the culture medium meets the standard, execute step S51; If the uniformity of the culture medium does not meet the standard, start the liquid adding component, perform the air intake and liquid supplement operation, start the vibration component again for secondary adjustment, and judge the uniformity of the culture medium until the uniformity of the culture medium meets the standard.

[0013] According to a preferred embodiment, step S1 further includes: The image data parameter is expressed as the image parameter of the culture dish with the culture medium added obtained by the vision module.

[0014] Compared with the prior art, the present invention has the following beneficial effects: 1. There are multiple vibration components set inside the incubator, and the culture dish is placed on the vibration components. In the vibration components, the air pump on the vibration base controls the inflation and deflation of the airbag through a connecting pipe, and together with the telescopic rods arranged diagonally, enables the vibration platform to achieve multi-dimensional vibration. At the same time, the movement of the electric slider on the arc-shaped slide rail and the interaction of the electromagnet and the magnetic ring further enhance the flexibility and controllability of the vibration. When the culture medium in the culture dish is unevenly distributed, this vibration component can generate vibrations with different frequencies and amplitudes, prompting the culture medium to flow and mix fully inside the culture dish, making nutrients and dissolved oxygen evenly distributed in each area, avoiding the growth limitation of microorganisms due to local nutrient deficiency or metabolite accumulation, thus significantly improving the consistency of microorganism growth and the culture efficiency, and providing a more stable and suitable growth environment for microorganisms.

[0015] 2. After the vision module obtains the image data of the culture medium in the culture dish, the detection module uses image recognition algorithms, cuckoo search algorithms, etc. to deeply analyze the data, accurately evaluate the uniformity of the culture medium from multiple dimensions, and compare it with a preset threshold. Once it detects that the uniformity of the culture medium does not meet the standard, the controller can quickly calculate the adjustment parameters of the vibration component and the liquid addition component according to the fuzzy logic algorithm, and automatically start the vibration or liquid supplement operation to achieve closed-loop control of the uniformity of the culture medium. This intelligent detection and regulation mechanism enables the operator to accurately and timely adjust the culture conditions without relying on subjective judgment based on experience, effectively improving the success rate of microorganism culture and the reliability of experimental results, and optimizing the microorganism culture process. Brief Description of the Drawings

[0016] Figure 1 is the structural schematic diagram of the present invention; Figure 2 is the structural schematic diagram after splitting the present invention; Figure 3 is the structural schematic diagram after splitting the vibration component; Figure 4 is the bottom view of the vibration platform; Figure 5 is the structural schematic diagram of the liquid addition component; Figure 6 is the structural schematic diagram after splitting the electric cylinder and the electric lifting rod; Figure 7 is the structural schematic diagram after splitting the rotating bracket and the support rod; Figure 8 is the structural schematic diagram after splitting the evaporation box and the cooling box; Figure 9 is the structural schematic diagram of the incubator; Figure 10 is Figure 7 the partial enlarged view of area a in Figure 11 isFigure 8 Partial enlarged view of area b; Figure 12 It is a schematic block diagram of the controller; Figure 13 It is a step flow chart for judging the uniformity of the culture solution.

[0017] 11. Incubator; 12. Mounting base; 13. Mounting groove; 14. Assembly seat; 21. Vibration base; 22. Vibration platform; 23. Support plate; 24. Airbag; 25. Telescopic rod; 26. Air pump; 27. Connecting rod; 28. Arc-shaped slide rail; 29. Electric slider; 31. Lighting panel; 32. Petri dish; 33. Vision module; 34. Controller; 401. Electric cylinder; 402. Electric lifting rod; 403. Mounting plate; 404. Suction cup; 405. Connecting sleeve; 406. Suction cup; 407. Rotating bracket; 408. Liquid adding part; 409. Support rod; 410. Liquid adding sleeve; 411. Rotating box; 412. First gear; 413. Second gear; 414. Fan blade; 501. Mounting table; 502. Evaporation box; 503. Support frame; 504. Water tank; 505. Heating module; 506. Steam generator; 507. Cooling box; 508. Partition plate; 509. Cooling box; 510. Collection box; 511. Flow guiding rib. Specific embodiments

[0018] The present invention will be specifically described below with reference to the accompanying drawings of the specification: As Figures 1 to 13 shown, the present invention provides a laboratory microorganism culture device with vibration assistance, including an incubator 11 and a liquid adding component for adding a culture solution. The incubator 11 serves as the main framework of the entire device, providing a relatively enclosed space environment for microorganism culture. The partition plate arranged inside it divides the incubator 11 into two culture chambers. This division method can realize the simultaneous culture of multiple microorganisms and avoid mutual interference between different microorganisms. The liquid adding component is located in the culture chamber and is mainly responsible for adding the culture solution to the petri dish 32 to ensure sufficient nutrient supply during the growth process of microorganisms.

[0019] There are multiple sets of mounting bases 12 provided inside the incubator 11. The mounting bases 12 provide a stable mounting foundation for the vibration components, enabling the vibration components to remain stable during operation and not easily displace. Multiple sets of vibration components inside the culture chamber are mounted on the mounting bases 12. The vibration components include a vibration base 21 and a vibration platform 22. The vibration base 21 is the basic support part of the entire vibration component, and the support plate 23 provided thereon is used to support the vibration platform 22. The vibration platform 22 and the support plate 23 are connected by multiple sets of air bags 24 and multiple sets of telescopic rods 25. The air bags 24 can be inflated and deflated under the action of an air pump 26. Cooperating with the telescopic rods 25, the vibration platform 22 can generate vibrations in different directions and amplitudes, prompting the culture solution in the culture dish 32 to flow and making the nutrients evenly distributed.

[0020] An illumination lamp board 31 is provided on the vibration platform 22. During the microbial culture process, the illumination lamp board 31 can provide sufficient light, facilitating the visual module 33 to observe the growth condition of the microorganisms in the culture dish 32. The culture dish 32 is placed on the vibration platform 22 and above the illumination lamp board 31. It is a carrier for carrying microorganisms and culture solution, providing a growth place for the microorganisms.

[0021] Multiple sets of visual modules 33 are provided inside the culture chamber. They are located above the culture dish 32 and can obtain the image information inside the culture dish 32 in real time, including the distribution state of the culture solution, the growth form of the microorganisms, etc. The visual module 33 can adopt a Basler acA2040-90um industrial camera. A detection module and a controller 34 for judging the uniformity of the culture solution are provided on the incubator 11. The detection module is electrically connected to the visual module 33. The detection module receives the image data transmitted by the visual module 33, analyzes and processes it, and judges the uniformity degree of the culture solution. The detection module can adopt a BACT / ALERT 3D detection module. The controller 34 then controls the operation of the liquid addition component and the vibration component according to the analysis result of the detection module. When the culture solution is uneven, the vibration component is started or the liquid addition component is controlled to supplement the culture solution, ensuring that the microorganisms can grow in a suitable environment. The controller 34 can adopt an Advantech ADAM-6050 controller.

[0022] Such as Figure 3 、 Figure 4As shown, the vibration assembly further includes multiple air pumps 26, which are installed on the vibration base 21 and serve as the power source for inflating and deflating the airbag 24. The air pump 26 is connected to the airbag 24 through a connecting pipe and can inflate or deflate the airbag 24 according to the instructions of the controller 34. When the airbag 24 is inflated, its volume expands and pushes the vibration platform 22 upward; when the airbag 24 is deflated, its volume shrinks, and the vibration platform 22 moves downward under the action of gravity and the telescopic rod 25. The multiple airbags 24 and the multiple telescopic rods 25 are diagonally arranged. This layout enables the vibration platform 22 to generate a certain torsional force while moving up and down, thereby achieving multi-dimensional vibration and more effectively promoting the mixing of the culture solution in the culture dish 32.

[0023] A connecting rod 27 is provided on the support plate 23, and both ends of the connecting rod 27 and the telescopic rod 25 are spherical. A first connecting block and multiple second connecting blocks are provided at the bottom of the vibration platform 22. One end of the connecting rod 27 is clamped on the first connecting block, enabling the vibration platform 22 to rotate around the connecting rod 27. This rotation function, combined with the action of the airbag 24 and the telescopic rod 25, further enriches the vibration form of the vibration platform 22, allowing the culture solution in the culture dish 32 to be stirred in different directions, which helps the nutrients to be more evenly distributed. One end of the telescopic rod 25 is clamped on the second connecting block. It not only supports the vibration platform 22 but also expands and contracts as the vibration platform 22 moves during the inflation and deflation of the airbag 24, ensuring the stable operation of the vibration platform 22 and restricting the movement range of the vibration platform 22 to prevent excessive vibration.

[0024] Two arc-shaped slide rails 28 are provided on the support plate 23, and the two sides of the two arc-shaped slide rails 28 are in contact with each other to form a circular slideway. The arc-shaped slide rail 28 provides a sliding track for the electric slider 29, and the electric slider 29 can move back and forth along the circular slideway on the arc-shaped slide rail 28. An electromagnet is provided on the electric slider 29, and a magnetic ring is provided at the bottom of the vibration platform 22. When the electric slider 29 moves on the arc-shaped slide rail 28, a magnetic force that interacts with each other is generated between the electromagnet and the magnetic ring. By controlling the magnitude and direction of the current of the electromagnet, the magnitude and direction of the magnetic force between the electromagnet and the magnetic ring can be changed, thereby adjusting the vibration amplitude and frequency of the vibration platform 22. For example, when a larger vibration amplitude is required, the current of the electromagnet is increased to enhance the magnetic force; when a smaller vibration amplitude is required, the current of the electromagnet is decreased to weaken the magnetic force. In this way, through the coordinated work of components such as the air pump 26, the airbag 24, the telescopic rod 25, the connecting rod 27, the arc-shaped slide rail 28, the electric slider 29, the electromagnet, and the magnetic ring, the vibration assembly can generate various forms and intensities of vibration to meet the requirements for the mixing of the culture solution during different microbial culture processes.

[0025] As Figure 2 、 Figure 5 、Figure 6 , Figure 9 The liquid adding assembly shown includes multiple sets of electric cylinders 401 and multiple sets of electric lifting rods 402. Multiple sets of installation grooves 13 are provided in the incubator 11, and the multiple sets of installation grooves 13 are respectively located in two culture cavities. The installation grooves 13 provide installation positions for the electric cylinders 401, enabling the electric cylinders 401 to be embedded in the incubator 11. The electric cylinders 401 are installed in the installation grooves 13, and a mounting plate 403 is provided at the bearing thereof. The electric cylinders 401 can drive the mounting plate 403 to rotate, thereby adjusting the angle of the components below the mounting plate 403 to meet the liquid adding requirements of different culture dishes 32. The electric lifting rods 402 are installed on the mounting plate 403 and are located above the culture dish 32. It can realize the lifting movement in the vertical direction, driving the connecting components to approach or move away from the culture dish 32.

[0026] An adsorption disc 404 is provided below the mounting plate 403. The shaft end of the electric lifting rod 402 passes through the mounting plate 403 and is connected to the adsorption disc 404. The adsorption disc 404 can move accordingly with the lifting of the electric lifting rod 402. Multiple sets of connecting sleeves 405 are provided at the bottom of the adsorption disc 404, and suction cups 406 are provided at the bottom of the connecting sleeves 405. The multiple suction cups 406 are interconnected through a ventilation pipe. Multiple negative pressure generators provided in the culture cavity are communicated with the ventilation pipe. When the negative pressure generators work, suction will be generated on the suction cups 406 through the ventilation pipe, thereby sucking up the top cover on the culture dish 32, facilitating the liquid adding assembly to add culture solution to the culture dish 32; at the same time, through setting, when the top cover of the culture dish 32 is sucked up, the overall stability of the culture dish 32 will not be affected.

[0027] As Figure 7 , Figure 10 shown, the liquid adding assembly further includes multiple sets of rotating brackets 407 and multiple sets of liquid adding members 408. The multiple sets of rotating brackets 407 are respectively located in the culture cavity and are installed on the inner wall of the incubator 11. As a support structure, they provide an installation basis for subsequent components. One end of the rotating bracket 407 is rotatably provided with a support rod 409, enabling the support rod 409 to rotate within a certain range, thereby flexibly adjusting the liquid adding position. One end of the support rod 409 is provided with a liquid adding sleeve 410, which serves to connect and support the liquid adding member 408.

[0028] The liquid adding member 408 is provided with a liquid inlet pipe and multiple groups of liquid outlet pipes, which is the key component for realizing the addition of the culture medium. A bearing is arranged inside the liquid adding sleeve 410, and the liquid inlet pipe is arranged through the bearing. The liquid adding member 408 is rotatably connected to the liquid adding sleeve 410. This connection method enables the liquid adding member 408 to rotate relative to the liquid adding sleeve 410, facilitating the adjustment of the liquid outlet direction. A liquid supply part is arranged on one side of the incubator 11, and the liquid adding sleeve 410 is connected to the liquid supply part through a liquid supply pipe. The liquid supply pipe is mutually communicated with the liquid adding sleeve 410 and the liquid adding member 408. The culture medium starts from the liquid supply part, enters the liquid adding member 408 through the liquid supply pipe and the liquid adding sleeve 410, and then is added into the culture dish 32 through the liquid outlet pipe, realizing the stable transportation and addition of the culture medium.

[0029] A rotating box 411 is arranged on one side of the liquid adding sleeve 410, and the rotating box 411 serves to accommodate and protect the internal components. The bearing arranged on the rotating box 411 provides support for the rotating rod, ensuring that the rotating rod can rotate smoothly. A first gear 412 is arranged below the rotating box 411, and the rotating rod arranged on the first gear 412 is arranged through the bearing, enabling the first gear 412 to form a rotating connection relationship with the rotating box 411. A second gear 413 is arranged on the liquid adding member 408, and the first gear 412 meshes with the second gear 413. When the first gear 412 rotates, it can drive the second gear 413 to rotate through gear meshing, thereby causing the liquid adding member 408 to produce a rotating action, making the culture medium spray more evenly.

[0030] A fan blade 414 is arranged inside the rotating box 411, and the fan blade 414 is sleeved on the rotating rod. The air pipe arranged above the rotating box 411 has its air outlet end facing the fan blade 414, and the fan arranged in the culture cavity is connected to the air pipe through the air inlet end of the air pipe. When the fan works, it blows air through the air pipe towards the fan blade 414. The fan blade 414 rotates under the action of the air flow, driving the rotating rod to rotate, and further driving the first gear 412 to rotate, finally realizing the rotation of the liquid adding member 408. By controlling the magnitude and direction of the wind force of the fan, the rotation speed and rotation direction of the fan blade 414 can be adjusted, and the rotation of the liquid adding member 408 can be controlled to meet the requirements for angles and positions when adding liquid to different culture dishes 32, enabling the culture medium to be smoothly added to the designated area of the culture dish 32.

[0031] Such as Figure 2 、 Figure 8 、 Figure 11As shown in the figure, an assembly seat 14 is provided on one side of the incubator 11. The assembly seat 14 serves as a basic carrier, providing an installation position for the humidification component and ensuring that the humidification component is firmly connected to the incubator 11. The humidification component provided on the assembly seat 14 includes an installation table 501 and an evaporation tank 502. The installation table 501 is connected to the assembly seat 14, playing a role of transitional support and at the same time providing a placement plane for the evaporation tank 502. A support frame 503 provided on the installation table 501, together with the installation table 501, limits and supports the evaporation tank 502. The evaporation tank 502 is installed on the installation table 501, located between the installation table 501 and the support frame 503, and is the core component for generating water vapor.

[0032] A water tank 504 provided on one side of the incubator 11 is used to store water. The water pump is connected to the water tank 504 and the evaporation tank 502 respectively through water pipes. The water pump can transport the water in the water tank 504 into the evaporation tank 502. Multiple heating modules 505 provided in the evaporation tank 502 work together with the steam generator 506. The heating modules 505 heat the water in the tank, and the steam generator 506 converts the water into water vapor, thereby providing a source of moisture for the incubator 11.

[0033] A cooling tank 507 provided above the support frame 503. Multiple partition plates 508 provided inside it divide the cooling tank 507 into multiple gas channels. These gas channels are the paths for the flow and cooling of water vapor. The cooling tank 507 is connected to the evaporation tank 502 through a delivery pipe, enabling the water vapor generated by the evaporation tank 502 to enter the cooling tank 507. Cooling tanks 509 filled with coolant are provided on both sides of the cooling tank 507, which can provide a cooling effect for the cooling tank 507, prompting the water vapor to cool down in the gas channels. Multiple turbine rotors are provided in the cooling tank 509, and the multiple turbine rotors are arranged in a staggered manner, strengthening the fluidity of the coolant in the cooling tank 509.

[0034] A collection box 510 provided in the cooling tank 507 is used to collect the condensed water droplets. Multiple guide ribs 511 provided on the inner wall of the gas channel, whose length is greater than the length of the partition plate 508, can guide the condensed water along the guide ribs 511 to the collection box 510 below. Finally, the top of the cooling tank 507 is connected to the incubator 11 through multiple delivery pipes, and the humid air after cooling treatment is transported into the incubator 11 to adjust the humidity environment in the incubator 11 and create suitable humidity conditions for microorganism culture.

[0035] The judgment of the uniformity of the culture medium by the detection module includes the following steps: S1: Obtain the image data parameters after adding the culture medium, analyze the image data parameters through an image recognition algorithm, and obtain the preliminary uniformity parameters; Specifically, during the microbial culture process, in step S1, the image data parameters after adding the culture medium are first obtained through the vision module 33. The vision module 33 is located above the culture dish 32 and can capture the state of the culture medium in the culture dish 32 in real time. In an optical imaging manner, it converts information such as the color distribution and surface morphology of the culture medium in the culture dish 32 into image data parameters, which contain the actual state information of the culture medium.

[0036] Subsequently, the system analyzes the image data parameters using an image recognition algorithm. The image recognition algorithm processes the image data parameters obtained by the vision module 33 and preliminarily judges the distribution of the culture medium in the culture dish 32 by comparing features such as the color depth and texture changes in different regions. For example, if the color distribution of the culture medium varies greatly, it may indicate a situation of uneven local concentration; if there is an obvious stratification in the surface morphology, it also reflects insufficient mixing of the culture medium. By analyzing these image features, a preliminary uniformity parameter is obtained, which can intuitively present the uniformity degree of the culture medium in the current state and provide basic data for subsequent more in-depth evaluation.

[0037] S2: Preset the uniformity standard parameter, compare the preliminary uniformity parameter with the uniformity standard parameter, and obtain the difference value parameter; Specifically, the uniformity standard parameter is a reference value set in advance according to the microbial culture requirements, which covers various index requirements such as the distribution of nutrient components and concentration gradient in the culture medium. After obtaining the preliminary uniformity parameter, the system compares it item by item with the uniformity standard parameter. By calculating the numerical gap between the preliminary uniformity parameter and the standard parameter in terms of indicators such as color distribution and concentration difference, the difference value parameter is obtained. This difference value parameter clearly shows the deviation degree of the actual uniformity state of the current culture medium from the ideal culture conditions and provides key data support for subsequent treatment of the culture medium.

[0038] S3: Based on the cuckoo search algorithm, perform iterative search and optimization on the difference value parameter to obtain an accurate evaluation parameter for the uniformity of the current culture medium; Specifically, in microbial culture, to more effectively master the uniformity of the culture medium, the cuckoo search algorithm can be used to process the difference value parameter. The cuckoo search algorithm is an optimization algorithm inspired by the breeding behavior of cuckoos and Levy flight, which can gradually find a better solution in a complex solution space.

[0039] In step S3, the iterative search and optimization of the difference value parameter based on the cuckoo search algorithm specifically includes: Initialize the cuckoo population, and use the difference value parameter as the initial solution space. Assume the difference value parameter is , including difference information in multiple dimensions, such as concentration differences and component ratio differences in different regions of the culture medium. Each cuckoo in the cuckoo population represents a possible solution, represented by a vector denoted as , being the number of cuckoos. For example, in the evaluation of the uniformity of the culture medium, may represent a set of hypothetical values regarding the distribution of each component of the culture medium.

[0040] Set the Levy flight strategy to simulate the process of cuckoos randomly searching for new nests, and generate new uniformity evaluation parameter solutions in each iteration. Levy flight is a random walk method with long-distance jump characteristics, and the formula for generating new solutions is: , where is the position of the th generation and the th cuckoo. When the step size control factor is appropriate, the cuckoo may jump from the current solution space position to a farther position to explore new possible solutions. According to the preset fitness function, comprehensively calculate the preliminary uniformity parameters

[0041] , the uniformity standard parameters and the generated new solutions to evaluate the fitness of each new solution. The fitness function can be defined as: , where and are weight coefficients used to adjust the importance of the preliminary uniformity parameters and the uniformity standard parameters in the evaluation; while

[0042] represents the possible solutions represented by each cuckoo in the cuckoo population, that is, in the process of evaluating the uniformity of the culture medium, a set of hypothetical values regarding the distribution of each component of the culture medium; it is the new uniformity evaluation parameter solution generated by the Levy flight strategy in each iteration. For example, in a certain iteration, x may represent the concentration distribution of a certain nutrient component in each region of the culture medium.

[0043] Retain the solution with the highest fitness as the next generation population, and randomly select some low-fitness nests for elimination, which are replaced by the newly generated solutions. This follows the principle of survival of the fittest, making the solutions in the population continuously evolve towards a better direction. Repeat the above steps of Levy flight, fitness evaluation, and population update until the preset number of iterations is reached or the convergence condition is met, and output the accurate evaluation parameters of the current uniformity of the culture medium. For example, when the change in fitness is less than a certain threshold in consecutive multiple iterations, the algorithm can be considered to have converged.

[0044] S4: Preset a threshold parameter and perform a comparison operation based on the accurate evaluation parameter and the threshold parameter; Specifically: The preset threshold parameter needs to be combined with the actual requirements of microbial culture. First, it is possible to refer to the experimental data of past similar microbial cultures and count at what level of the culture medium uniformity the microbial growth state is good, so as to determine a basic threshold range. Second, consider the characteristics of the cultured microorganisms, such as their sensitivity to changes in the concentration of nutrients. If the microorganisms are sensitive to concentration changes, the threshold range should be set smaller; otherwise, it can be appropriately relaxed. For example, for some microorganisms with strong adaptability to nutrient concentration fluctuations, the threshold can be relatively wide. Finally, through preliminary experiments, different thresholds can be set for testing, observing the growth of microorganisms, and finally determining the appropriate threshold parameter, and then comparing it with the accurate evaluation parameter to judge whether the uniformity of the culture medium meets the standard.

[0045] S51: If the accurate evaluation parameter is greater than the threshold parameter, it is determined that the uniformity of the culture medium meets the standard, and then start the normal culture program of the device to allow the microorganisms to be cultured in the current state; Specifically: When the accurate evaluation parameter obtained by iteratively optimizing through the cuckoo search algorithm is greater than the preset threshold parameter, it means that the current uniformity of the culture medium can meet the basic conditions for microbial culture. At this time, the device will automatically start the normal culture program, providing suitable environmental conditions such as temperature and light for the microorganisms, and allowing the microorganisms to grow naturally in such a culture medium environment. For example, for the common culture of Escherichia coli, when the distribution of nutrients in the culture medium is relatively uniform and the accurate evaluation parameter shows that the requirements are met, the device turns on functions such as constant temperature and light to enable the normal reproduction of Escherichia coli.

[0046] S52: If the accurate evaluation parameter is less than the threshold parameter, it is determined that the uniformity of the culture medium does not meet the standard, and perform an operation to make the culture medium uniform.

[0047] Specifically: When it is found that the accurate evaluation parameter is less than the threshold parameter, it indicates that the current uniformity of the culture medium is insufficient. First, according to the difference between the accurate evaluation parameter and the threshold parameter, use the fuzzy logic algorithm to calculate the vibration frequency and amplitude adjustment coefficient of the vibration component. Then, control the air pump 26 to change the inflation or deflation frequency of the airbag 24 according to the vibration frequency adjustment coefficient, and the electric slider 29 and the electromagnet cooperate to control the amplitude of the vibration platform 22, and turn on the vibration component to perform the first shaking and stirring of the culture medium in the culture dish 32. After the stirring is completed, re-execute steps S1 - S4 to judge the uniformity. If it still does not meet the standard, start the liquid addition component to supplement the liquid, and then use the vibration component for secondary adjustment until it meets the standard.

[0048] The operation to make the culture medium uniform includes: According to the difference between the accurate evaluation parameter and the threshold parameter, the vibration frequency and amplitude adjustment coefficient of the vibration component are calculated using the fuzzy logic algorithm. Let the accurate evaluation parameter be , and the threshold parameter be , and the difference . The fuzzy logic algorithm processes through a series of fuzzy rules to obtain the vibration frequency adjustment coefficient and the amplitude adjustment coefficient . For example, when is relatively large, it means that the uniformity of the culture medium is relatively poor, and the fuzzy logic algorithm will output larger and values.

[0049] Control the air pump 26 to adjust the inflation or deflation frequency of the airbag 24 according to the vibration frequency adjustment coefficient, and cooperate with the electromagnet through the electric slider 29 to control the amplitude of the vibration platform 22, and start the vibration component to perform the initial shaking and stirring of the culture medium in the petri dish 32. The inflation or deflation frequency of the air pump 26 , where is the initial frequency. The electric slider 29 and the electromagnet adjust the amplitude of the vibration platform 22 according to to make the culture medium shake sufficiently in the petri dish 32.

[0050] After the initial shaking and stirring is completed, repeat the operations in steps S1 - S4, and re - judge the uniformity of the culture medium. Obtain the image data parameters after adding the culture medium again, analyze them through the image recognition algorithm to obtain the preliminary uniformity parameter, compare it with the preset uniformity standard parameter to obtain the difference value parameter, then use the cuckoo search algorithm to iteratively optimize to obtain the new accurate evaluation parameter, and finally compare it with the threshold parameter.

[0051] If the uniformity of the culture medium meets the standard, execute step S51 to start the normal culture program; if the uniformity of the culture medium does not meet the standard, then start the liquid adding component to perform the liquid supplement operation, start the vibration component again for secondary adjustment, and judge the uniformity of the culture medium until the uniformity of the culture medium meets the standard. Through such continuous adjustment and judgment, it is ensured that the culture medium can reach a uniform degree suitable for microorganism culture.

[0052] It should be noted that, in order to simplify the description of the present invention disclosure and thus help the understanding of one or more embodiments of the invention, in the previous description of the embodiments of the present invention, sometimes multiple features are merged into one embodiment, drawing, or description thereof.

Claims

1. A microbial culture device for laboratory use with vibration assistance, comprising an incubator (11) and a liquid adding component for adding culture medium, characterized in that: Two culture chambers are formed by a partition plate arranged inside the incubator (11), the liquid adding component is located in the culture chamber, multiple mounting seats (12) are arranged inside the incubator (11), multiple vibration components are arranged in the culture chamber, and the vibration components are mounted on the mounting seats (12); The vibration component includes a vibration base (21) and a vibration platform (22), a support plate (23) is arranged on the vibration base (21), the vibration platform (22) is located above the support plate (23), and the vibration platform (22) and the support plate (23) are connected by multiple air bags (24) and multiple telescopic rods (25); A lighting panel (31) is arranged on the vibration platform (22), and a culture dish (32) is placed on the vibration platform (22) and above the lighting panel (31); Multiple visual modules (33) are arranged in the culture chamber, the visual modules (33) are located above the culture dish (32), a detection module and a controller (34) for judging the uniformity of the culture medium are arranged on the incubator (11), and the detection module is electrically connected to the visual module (33).

2. The microbial culture device for laboratory use with vibration assistance according to claim 1, characterized in that: The vibration component further includes multiple air pumps (26), the air pumps (26) are mounted on the vibration base (21) and are connected to the air bags (24) through connecting pipes, and the multiple air bags (24) and the multiple telescopic rods (25) are arranged diagonally; A connecting rod (27) is arranged on the support plate (23), one ends of the connecting rod (27) and the telescopic rod (25) are both spherical, a first connecting block and multiple second connecting blocks are arranged at the bottom of the vibration platform (22), one end of the connecting rod (27) is clamped on the first connecting block, the vibration platform (22) rotates around the connecting rod (27) as the center, and one end of the telescopic rod (25) is clamped on the second connecting block; Two arc-shaped slide rails (28) are arranged on the support plate (23), two sides of the two arc-shaped slide rails (28) are in contact with each other to form a circular slideway, and an electric slider (29) is slidably arranged on the arc-shaped slide rail (28), and the electric slider (29) moves back and forth along the circular slideway; An electromagnet is arranged on the electric slider (29), and a magnetic ring is arranged at the bottom of the vibration platform (22).

3. The microbial culture device for laboratory use with vibration assistance according to claim 1, characterized in that: The liquid adding assembly includes multiple sets of electric cylinders (401) and multiple sets of electric lifting rods (402). Multiple sets of mounting grooves (13) are formed in the incubator (11), and the multiple sets of mounting grooves (13) are respectively located in two sets of the culture cavities. The electric cylinders (401) are installed in the mounting grooves (13). The electric cylinders (401) are provided with a mounting plate (403) through bearings. The electric lifting rods (402) are installed on the mounting plate (403) and are located above the culture dish (32). An adsorption disc (404) is arranged below the mounting plate (403). The shaft end of the electric lifting rod (402) passes through the mounting plate (403) and is connected to the adsorption disc (404). Multiple sets of connecting sleeves (405) are arranged at the bottom of the adsorption disc (404). Suction cups (406) are arranged at the bottom of the connecting sleeves (405). The multiple sets of suction cups (406) are mutually communicated through a ventilation pipe. Multiple sets of negative pressure generators are arranged in the culture cavity, and the negative pressure generators are communicated with the ventilation pipe.

4. The microbial culture device for laboratory with vibration assistance according to claim 3, wherein: The liquid adding assembly further includes multiple sets of rotating brackets (407) and multiple sets of liquid adding members (408). The multiple sets of rotating brackets (407) are respectively located in the culture cavity and are installed on the inner wall of the incubator (11). One end of the rotating bracket (407) is rotatably provided with a support rod (409), and a liquid adding sleeve (410) is arranged at one end of the support rod (409). The liquid adding member (408) is provided with a liquid inlet pipe and multiple sets of liquid outlet pipes. A bearing is arranged in the liquid adding sleeve (410), and the liquid inlet pipe is arranged in the bearing. The liquid adding member (408) is rotatably connected to the liquid adding sleeve (410). A liquid supply part is arranged on one side of the incubator (11). The liquid adding sleeve (410) is connected to the liquid supply part through a liquid supply pipe, and the liquid supply pipe is mutually communicated with the liquid adding sleeve (410) and the liquid adding member (408).

5. The microbial culture device for laboratory with vibration assistance according to claim 4, wherein: A rotating box (411) is arranged on one side of the liquid adding sleeve (410). The rotating box (411) is provided with the bearing. A first gear (412) is arranged below the rotating box (411). A rotating rod is arranged on the first gear (412), and the rotating rod is arranged in the bearing. The first gear (412) is rotatably connected to the rotating box (411). A second gear (413) is arranged on the liquid adding member (408), and the first gear (412) is meshed with the second gear (413). A fan blade (414) is arranged in the rotating box (411). The fan blade (414) is sleeved on the rotating rod. An air pipe is arranged above the rotating box (411), and the air outlet end of the air pipe faces the fan blade (414). A blower is arranged in the culture cavity, and the air inlet end of the air pipe is connected to the blower.

6. A microbial culture device for laboratory use with vibration assistance according to claim 1, characterized in that: An assembly seat (14) is provided on one side of the incubator (11), a humidification component is provided on the assembly seat (14), the humidification component includes a mounting table (501) and an evaporation tank (502), the mounting table (501) is connected to the assembly seat (14), a support frame (503) is provided on the mounting table (501), and the evaporation tank (502) is installed on the mounting table (501) and is located between the mounting table (501) and the support frame (503); A water tank (504) and a water pump are provided on one side of the incubator (11), the water pump is connected to the water tank (504) and the evaporation tank (502) respectively through water pipes, and multiple heating modules (505) and a steam generator (506) are provided in the evaporation tank (502); A cooling box (507) is provided above the support frame (503), multiple partition plates (508) are provided in the cooling box (507), multiple gas channels are formed in the cooling box (507) through the multiple partition plates (508), and the cooling box (507) communicates with the evaporation tank (502) through a delivery pipe; Cooling boxes (509) filled with coolant are provided on both sides of the cooling box (507), a collection box (510) is provided in the cooling box (507), multiple flow guiding ribs (511) are provided on the inner walls of the gas channels, the length of the flow guiding ribs (511) is greater than the length of the partition plates (508), and the collection box (510) is located below the flow guiding ribs (511); The top of the cooling box (507) communicates with the incubator (11) through multiple delivery pipes.

7. A microbial culture device for laboratory use with vibration assistance according to claim 1, characterized in that, The judgment of the uniformity of the culture medium by the detection module includes the following steps: S1: Obtain the image data parameters after adding the culture medium, analyze the image data parameters through an image recognition algorithm, and obtain the preliminary uniformity parameters; S2: Preset the uniformity standard parameters, compare the preliminary uniformity parameters with the uniformity standard parameters, and obtain the difference value parameters; S3: Perform iterative search and optimization operations on the difference value parameters based on the cuckoo search algorithm to obtain the accurate evaluation parameters of the current culture medium uniformity; S4: Preset the threshold parameters, and perform a comparison operation based on the accurate evaluation parameters and the threshold parameters; S51: If the accurate evaluation parameter is greater than the threshold parameter, it is determined that the uniformity of the culture medium meets the standard, and the normal culture program of the device is started to culture the microorganisms in the current state; S52: If the accurate evaluation parameter is less than the threshold parameter, it is determined that the uniformity of the culture medium does not meet the standard, and the culture medium uniformity treatment operation is performed.

8. A microorganism culture device for laboratory use with vibration assistance according to claim 7, characterized in that, In step S3, the iterative search and optimization operations on the difference value parameters based on the cuckoo search algorithm specifically include: Initialize the cuckoo population, and use the difference value parameters as the initial solution space; Set the Levy flight strategy, simulate the process of the cuckoo randomly searching for a new nest, and generate a new solution of the uniformity evaluation parameter in each iteration; According to a preset fitness function, the preliminary uniformity parameter, the uniformity standard parameter and the generated new solutions are comprehensively calculated to evaluate the fitness of each new solution; The solution with the highest fitness is retained as the next-generation population, and some nests with low fitness are randomly selected for elimination and replaced by the newly generated solutions; Repeat the above steps of Levy flight, fitness evaluation and population update until the preset number of iterations is reached or the convergence condition is satisfied, and output the accurate evaluation parameters of the current culture medium uniformity.

9. The microbial culture device for laboratory use with vibration assistance according to claim 7, characterized in that, The culture medium uniform treatment operation includes: According to the difference between the accurate evaluation parameter and the threshold parameter, the vibration frequency and the amplitude adjustment coefficient of the vibration component are calculated by using a fuzzy logic algorithm; Control the air pump (26) to adjust the inflation or deflation frequency of the airbag (24) according to the vibration frequency adjustment coefficient, and cooperate with the electromagnet through the electric slider (29) to control the amplitude of the vibration platform (22), and start the vibration component to perform the first shaking and stirring on the culture medium in the culture dish (32); After the first shaking and stirring is completed, repeat the operations in steps S1-S4, and re-judge the culture medium uniformity; If the culture medium uniformity meets the standard, execute step S51; If the culture medium uniformity does not meet the standard, start the liquid adding component, perform the air intake and liquid supplement operation, start the vibration component again for secondary adjustment, and judge the culture medium uniformity until the culture medium uniformity meets the standard.

10. A microbial culture device for laboratory use with vibration assistance according to claim 7, characterized in that, Step S1 also includes: The image data parameter is represented as the image parameter of the culture dish (32) with the culture medium added obtained by the vision module.

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